Dynamica HALO DB-20 User manual

MODEL HALO DB-20 UV-VIS SPECTROPHOTOMETER
HALO DB-20
Spectrophotometer
Instruction Manual
Please read through this manual carefully and store it
in a safe place.
 Before using the instrument, please read the safety
instructions and precautions carefully
 Keep this manual in a safe place nearby to access

INSTRUCTION MANUAL
HALO DB-20
UV-Vis Spectrophotometer
Read and keep this manual
· Read the safety instructions carefully and
understand them before using the
equipment.
· Keep this manual at hand for reference.
Dynamica Ltd.
Version No. V1.1

CONTENTS
PREFACE……………...……….………..…….…….………PREFACE-1
IMPORTANT……………...……….………..….…….…IMPORTANT-1
Precautions on Electromagnetic
Wave Interference……………………………………………………………………IMPORTANT-1
1. Possible Electromagnetic Wave Interference
Caused by This Instrument………………………………………………………..IMPORTANT-1
2. Possible Electromagnetic Wave Interference
Affecting This Instrument…………………………………………………………..IMPORTANT-1
Warranty on Product………………………………………………………..………IMPORTANT-2
Technical Service……………………..……………………………………………IMPORTANT-4
Disposal this instrument……………………………………………………..……IMPORTANT-5
Other Precautions…………………………………………………………..………IMPORTANT-5
1. Handling of Chemicals and Samples…........................................... …IMPORTANT-5
2. Notice on Instruction Manuals ........................................................ …IMPORTANT-5
3. Trademark Acknowledgments….……………………………………IMPORTANT-5
SAFETY SUMMARY……………...……….……….SAFETY-1
THE PERSONAL COMPUTER…………………..……………SAFETY-10
COMPUTER VIRUSES AND
BACKUP DATA………………………….………………………….SAFETY-10
WARNING DISPLAY LABELS…………………………………….SAFETY-11
CHAPTER 1 INSTALLATION………………….……………………….1-1
1.1 UNPACKING…………………………………………………..…………………………1-1
1.2 INSTALLATION SITE………………….……………………………………...………..…1-2
1.2.1 Installation Conditions…………………….………….…………………………….1-2
1.2.2 Installation Environment……………………………….…………………………..1-2
1.3 CHECK OF CONTENTS……………………………...……………………………………1-3
1.4 CHECK OF VOLTAGE AND FUSE……………………………...………………………….1-4
1.5 SIDE PANEL OF THE DB-20………………………..………..………………………1-5
1.6 CONNECTION OF POWER CORD AND GROUNDING WIRE……………...…………………1-5
1.7 CONNECTION OF PRINTER………………………...……………………………………..1-6
1.8 LOCAL-REMOTE SWITCH…………………….………………………………………….1-7
1.9 LCD BRIGHTNESS ADJUSTMENT PORT…………………………………………………1-7
I

1.10 CONNECTING ACCESSORY TO DB-20……………….………………..…………….1-8
1.11 CONNECTING DB-20 TO PC SOFTWARE………………….……..………………1-8
1.12 CONNECTING USB FLASH MEMORY…..……………………………………………….1-8
CHAPTER 2 FUNCTION…………………………………………………2-1
2.1 APPLICATION……………………………………………………………………………2-1
2.2 PRINCIPLE OF OPERATION……………………………………………………………….2-2
2.2.1 Optical System……………………………………………….……………………..2-2
2.2.2 Signal Processing and Control System……………………………………………..2-3
2.3 PROPER USE OF SPECTROPHOTOMETER…………………………………...…………….2-3
2.3.1 Solvent Selection…………………………………………………..………………2-3
2.3.2 Special Samples……………………………………………………………………2-4
2.4 FUNCTIONS…………………………………………………………………2-4
2.4.1 Quantitative Analysis……………………………………………..………………2-4
2.4.2 Wavelength Scan…………………………………………………….…………….2-5
2.4.3 Time Scan…………...……………………………………………….…………….2-6
2.4.4 Secondary Processing Functions…………………………………….…………….2-6
2.4.5 Data Storage Function…………..……………………………………………….2-7
2.4.6 Automatic Start Function………………..……………………………………….2-8
2.4.7 Automatic Calibration and Self – Diagnosis Functions……………………….2-8
2.5 SPECIFICATION………………………………………………………………………….2-8
CHAPTER 3 OPERATIONAL METHOD…………………………………3-1
3.1 BASIC OPERATION……………………………………………………………………....3-1
3.1.1 Operational Procedure……………………………………………………………..3-1
3.1.2 Key-Panel Functions………………………………………………………….…3-1
3.2 PRELIMINARY OPERATION………………………………………………………………3-3
3.2.1 Initialization………………………………………………………………………..3-3
3.2.2 Main Menu…………………………………………………………………………3-4
3.3 PHOTOMETRY……………………………………….…………………………………..3-5
3.3.1 Step by Step Operation……………………………...……………………………..3-6
3.3.2 Performing Photometric Measurements………………………..……………….3-12
3.4 WAVELENGTH SCAN…………………………………………………..………….3-20
3.4.1 Measurement Parameter Setting………………………………………………….3-20
3.4.2 Performing a Wavelength Measurement…………...…………………………….3-28
II

3.4.3 Secondary Processing…………………………………………...………………..3-29
3.4.4 Wavelength Scan Sampling Interval List…..…………………...………………..3-33
3.5 TIME SCAN………………………………………...…………………………………..3-34
3.5.1 Measurement Conditioning Setting……………………………..….…………….3-34
3.5.2 Performing a Time Scan Measurement…………………….…………….………3-38
3.5.3 Secondary Processing……………………………………….……………………3-39
3.6 MULTIPLE WL…………………………………………………………………………3-46
3.6.1 Measurement Conditions Setting……………………………………..…………..3-46
3.6.2 Performing Multiple WL Measurement…………………………………….……3-53
3.7 FILE MANAGER………………………………………………………………...………3-55
3.7.1 Inside Memory…………………………………………………………..………..3-55
3.7.2 Flash Memory…………………………………………...………………………..3-56
3.8 CALIBRATING THE WAVELENGTH…………………………………………………...…3-58
3.8.1 Calibrating the Wavelength………………………………………………………3-58
3.8.2 Set Time……………………………………………………………………..……3-59
3.8.3 Recording Lamp Usage…………………………………………………….…….3-60
3.8.4 GLP/GMP…………………………………………………………………...……3-60
3.9 TEST MENU…………………………………………………………………………..3-65
3.9.1 Retrieving a Test Stored in Inside Memory………………………….…..3-65
3.9.2 Deleting Test From Inside Memory………………………………………3-65
3.9.3 Autostart Function………………………………………………………………3-66
CHAPTER 4 MAINTENANCE…………………..………….…………….4-1
4.1 SELF DIAGNOSIS AND AUTOMATIC ADJUSTMENT………………….….………………..4-1
4.2 CONFIRMATION OF SPECIFICATIONS…………………………….….….……..…………4-1
4.2.1 Wavelength Accuracy…………………………………………..….….…………4-1
4.2.2 Spectrum Bandwidth………………………………………….…...……………4-2
4.2.3 Check for Baseline Flatness……………………………………..……………….4-2
4.2.4 Confirmation of Baseline Flatness……………………………..…………………4-3
4.3 PERIODIC INSPECTION………………………………………………..…….………….4-4
4.3.1 Cleaning Sample Compartment……………………………….….………………4-4
4.3.2 Cleaning the Sample Compartment…………………………….….……………..4-6
4.4 TROUBLESHOOTING………………………………………………….….……………..4-7
CHAPTER 5 REPLACEMENT OF PARTS…………………….……….5-1
5.1 CONSUMABLES AND SPARE PARTS ……………………………………………………..5-1
III

5.2 PARTS REPLACEMENT…………………………….………………….………………….5-1
5.2.1 Replacement of Light Source Lamp…………………………...…………………..5-1
5.2.2 Change Source Lamp Usage Time………..….……………………………………5-4
Notice……...………………………. …………….…………………….………. A
IV

PREFACE
Thank you very much for your purchase of Dynamica HALO DB-20
Spectrophotometer.
This instruction manual has been prepared for users of the DB-20.
It describes how to handle the Spectrophotometer.
The HALO DB-20 Spectrophotometer is intended for the purpose of
photometric analysis. Do not use it for any other purpose.
The HALO DB-20 is designed for use by persons having a basic
knowledge of chemical analysis. Other persons should operate the
instrument in the presence of someone who has such basic
knowledge. Remember that improper use of the instrument,
chemicals or samples would result not only in wrong analytical data
but in consequences adverse to safety.
Before using the instrument, be sure to read through this instruction
manual to enable correct use.
For installation/maintenance of the instrument, read the instruction
manual carefully to attain a full understanding of the instructions.
Be sure to observe cautionary instructions.
Keep this manual handy for easy reference when necessary.
Information contained in this manual is subject to change without
notice for product improvement.
ABOUT THIS MANUAL
Before using the instrument, be sure to read through this
instruction manual. First of all, read “SAFETY SUMMARY”
included at the beginning of this manual for ensuring safety in
operation of the spectrophotometer main unit.
PREFACE-I

IMPORTANT
Precautions on Electromagnetic Wave Interference
1. Possible Electromagnetic Wave Interference Caused by This Instrument
When this instrument is used in a residential area or an adjacent
area thereto, it may cause interference to radio and television
reception.
To prevent this, use the specified system connection cables in strict
accordance with the instruction manual. The instrument is
designed to minimize possible electromagnetic wave interference
caused by it if the specified cables are connected properly.
However, there is no guarantee that electromagnetic wave
interference will not be caused by the instrument. If the instrument
does cause interference to radio or television reception, which can
be determined by turning off and on the instrument, the user is
encouraged to try to correct the interference by one or more of the
following measures:
•Increase separation between the instrument and radio/TV
receiver.
•Connect the instrument to an outlet on a circuit different from
that to which the radio/TV receiver is connected.
2. Possible Electromagnetic Wave Interference Affecting This Instrument
If this instrument is used near an intense electromagnetic source,
interference noise may be given to the instrument to incur an
adverse effect on its performance or functionality.
To prevent this, use the specified system connection cables in strict
accordance with the instruction manual. The instrument is
designed to minimize possible electromagnetic wave interference
affecting it if the specified cables are connected properly.
However, there is no guarantee that electromagnetic wave
interference will not occur in this instrument. If the instrument does
incur electromagnetic wave interference, which can be determined
by turning on and off possible sources of electromagnetic wave
interference nearby, the user is encouraged to try to correct the
interference by one or more of the following measures:
IMPORTANT-1

•Reorient the instrument.
•Increase separation between the instrument and possible
sources of electromagnetic wave interference.
•Increase separation between the power cable of the instrument
and possible sources of electromagnetic wave interference.
•Connect the instrument to an outlet on a circuit different from
that to which possible sources of electromagnetic wave
interference are connected.
•Confirm that any other device connected with the instrument is
not affected by electromagnetic wave interference.
Warranty on Product
The Model HALO DB-20 Spectrophotometer is warranted to
operate according to the specifications given in the instruction
manual, provided it is used in accordance with the instructions
described in the manual.
(1) Scope of Warranty
(a) Any parts which prove to be defective in design or
workmanship during the warranty period will be repaired
without charge.
(b) A substitute part may be used for repair, or replacement
with an equivalent product may be made instead of repair.
(c) Such system components as a personal computer and
printer to be updated frequently for improvement may not
be available in original versions at the time of
replacement.
(2) Warranty Period
One year from the date of initial installation.
Consumables such as WI and D2 lamps are excluded from
warranty.
(3) Availability of Technical Support Service
Technical support service for this instrument is available within
regular working hours on workdays predetermined by us.
IMPORTANT-2

(4) Limitations and Exclusions on Warranty
Note that this warranty is void in the following cases, even if
they occur within the warranty period.
(a) Failure due to operation at a place not meeting the
installation requirements specified by us
(b) Failure due to power supply voltage/frequency other than
specified by us or due to abnormality in power supply
(c) Corrosion or deterioration of the tubing due to impurities
contained in reagent, gas, air or cooling water supplied
by the user
(d) Corrosion of the electric circuits or deterioration of the
optical elements due to highly corrosive atmospheric gas
(e) Failure due to use of hardware, software or spare parts
other than supplied by us
(f) Failure due to improper handling or maintenance by the
user
(g) Failure due to maintenance or repair by a service agent
not approved or authorized by us
(h) After disposal of this instrument, or after its resale without
our approval
(i) Failure due to relocation or transport after initial
installation
(j) Failure due to disassembly, modification or relocation not
approved by us
(k) Consumables, and failure of parts that have reached the
end of specified useful life
(l) Failure of parts excluded from the warranty in the
instruction manual or other documents
(m) Failure due to acts of God, including fire, earthquake,
storm, lightning, social disturbance, riot, crime,
insurrection, war (declared or undeclared), radioactive
pollution, contamination with harmful substances, etc.
IMPORTANT-3

(n) Failure of the hardware, or damage to the system
software, application software, data or hard disk due to
computer virus infection
(o) Failure of the personal computer connected with the
instrument, or damage to the system software,
application software, data or hard disk due to power
interruption or momentary power voltage drop caused by
lightning or the like
(p) Failure of the personal computer connected with the
instrument, or damage to the system software,
application software, data or hard disk due to
disconnection of main power to the personal computer
without taking the specified normal shutdown procedure
(5) Disclaimer of Warranty
(a) Any express warranties other than the explicit conditions
indicated in (1) are excluded from this warranty.
Any other implied warranties of merchantability and
fitness for a particular purpose are not included in this
warranty. No liability is assumed for direct or indirect
damages arising out of explicit or implied warranties.
(b) Oral or written information or advice given by our dealers,
distributors, agents or employees without our express
permission shall not create a warranty or in any way
increase the scope of this warranty.
Technical Service
Installation of this instrument shall be carried out by or under
supervision of qualified service personnel of Dynamica or its
authorized service agent.
Before installation of the instrument, the user shall make
preparations for satisfying the installation requirements in
accordance with the instruction manual.
If relocation of the instrument becomes necessary after initial
installation (delivery), please notify your local sales representative
or nearest service office of Dynamica.
IMPORTANT-4

Disposal this instrument
When you discard equipment, please check and discard a related
statute etc. or ask the service section of Dynamica.
Other Precautions
1. Handling of Chemicals and Samples
(1) The user is responsible for following relevant legal standards
and regulations in handling, storage and discarding of
chemicals and samples used in analytical operations of this
instrument.
(2) Reagents, standard solutions and accuracy-control samples
shall be handled, stored and discarded as instructed by the
respective suppliers.
2. Notice on Instruction Manuals
(1) Information contained in the instruction manuals furnished
with the instrument is subjected to change without notice for
product improvement.
(2) This manual is copyrighted by Dynamica with all rights
reserved.
(3) No part of this manual may be reproduced or transmitted in
any form or by any means without our express written
permission.
3. Trademark Acknowledgments
Microsoft, Windows, Microsoft Excel, Microsoft Word and Windows
XP are registered trademarks, trademarks or trade names of
Microsoft Corporation, USA, while other company names and
product names are those of respective companies.
IMPORTANT-5

SAFETY SUMMARY
For your safety please read the following precautions carefully
before using the DB-20 UV-Vis Spectrophotometer.
General Safety Guidelines
 For safe handling of this product, please follow the instruction
procedure in the manual for this product.
 Pay special attention to follow all the hazard warnings on the product
and in the manual. Failure to do so can cause injury to you or damage
to the product.
 After installation, please do not move the equipment. A vibration might
affect the adjustment of the product.
 The hazard warnings, which appear on the warning labels on the
product or in the manual, have one of the following alert headings
consisting of an alert symbol and a signal word, DANGER, WARNING,
or CAUTION.
DANGER: Indicates an imminently hazardous situation that, if not
avoided, will result in death or serious injury.
(It does not apply to this equipment.)
WARNING: Indicates a potentially hazardous situation that, if not
avoided, can result in death or series injury.
CAUTION: Indicates a hazardous situation that, if not avoided,
will or can result in minor to moderate injury, or
serious damage to the product.
:The alert symbol shown precedes every signal word fo
r
hazard warnings, and appears in safety related
descriptions in the manual.
SAFETY-1

SAFETY SUMMARY (Continued)
General Safety Guidelines (Continued)
In addition, the following “Attention” and “Note” are not directly related to the safety of a person:
SAFETY-2
Attention: It is used to present warnings, which are not
directly related to personal injury hazards. It is
used to indicate prevention against damage to the
equipment.
Note: This is used to indicate instructions that enable you
to operate the equipment accurately and perform
accurate measurements.

SAFETY SUMMARY (Continued)
General Safety Guidelines (Continued)
Before Using
 Before using this product, please make sure you read and understand the
instructions.
 Please keep this manual in a safe and easily accessible place so that you can use it
when necessary.
 Please make sure to use this product properly and follow the instructions as
specified in this manual.
 Please make sure to understand and follow the instructions regarding safety in this
manual.
 If you do not follow the instructions in this manual, an inaccurate analysis may result
or bodily injury may occur.
 Because of danger, please make sure not to modify or alter the product, make sure
not to use unspecified parts, and make sure not to operate the equipment by
removing/defeating the safety device(s).
 When using chemicals, please make sure to ventilate the room well. If there is not
enough ventilation, it may be hazardous to your health.
 Although we have carefully considered the instructions written on the products and
manuals, it is possible for an unexpected event to occur. When operating the
equipment, aside from following the instructions, be very cautious.
SAFETY-3

SAFETY SUMMARY (Continued)
General Safety Guidelines (Continued)
Precautions for Installation • Maintenance • Relocation and After Sale
Technical Service
 Before installation, confirm that there are no missing items or
standard accessories. If there is something missing or damaged, or
you have noticed any problems, please contact our nearest
representative.
 Operating the equipment without a standard part can damage the
equipment and cause safety concerns. If that occurs, please follow
the instruction of the installer.
 Installation of this instrument shall be carried out by or under
supervisions of qualified service personnel of Dynamica or its
authorized service agent.
 When relocation of this instrument becomes necessary after initial
installation (delivery), please notify your local Dynamica sales
representative or nearest Dynamica service office. Technical support
service for this instrument is available from s service agent approved
or authorized by Dynamica within regular working hours or workdays.
 Please do not perform any other operations that are not included in
the manual. If any problem occurs with the equipment, please contact
the agent from whom you purchased it or the service department of
Dynamica.
SAFETY-4

SAFETY SUMMARY (Continued)
WARNING: Poisoning from Organic Solvent Gas
Handling Organic Solvents
 The organic solvent vapor may be harmful to your health.
WARNING: Eye Injury from Organic Solvents
Handling Organic Solvents
 Please wear protective glasses when using organic solvents. If the
organic solvent should get into your eye, flush your eye immediately
under running water for at least 5 minutes while keeping your eyelids
open. See a physician for appropriate treatment.
SAFETY-5

SAFETY SUMMARY (Continued)
WARNING: Electrical Shocks from Improper Grounding
Electrical Shock from Improper Grounding
 When wiring the personal computer, power supply for the thermostatic
cell holder and the like, please make sure to use the 3-prong wire (with
ground) provided.

WARNING: Electrical Shock
Electrical Shock from Contact
 High voltage is used inside of the equipment. Never remove the cover
except by a service person or as instructed in the section for lamp
replacement.
 When cleaning the inside of the cell compartment, be sure to turn off the
unit.
CAUTION: High Temperature
Burns from high temperatures
 The lamp will become very hot during operation, please make sure that
the D2 lamp and the tungsten lamp is cooled off when replacing the lamp.
SAFETY-6

SAFETY SUMMARY (Continued)
CAUTION: Injury from incidental contact with the unit.
Handling the sample compartment cover
 When you open and close the sample compartment cover, please be
careful not to get your hand or hair caught. Please assure your safety
before operation.
CAUTION: Fatigue due to Prolonged Work
Data Processing
 Viewing the display in your work can cause eye and physical fatigue if
you continue to work in the same posture for extended periods.
 When working with the display for a prolonged period, for your health,
make sure to take breaks for 10 to 15 minutes every hour in order to rest
your eyes and body.
SAFETY-7

SAFETY SUMMARY (Continued)
NOTE
Precautions regarding Accuracy and Precision of Measurements
 Do a routine check prior to taking measurements. In order to obtain
accurate measuring results, please perform tests as instructed, following
the instructions in Chapter 3.
SAFETY-8