Dynamica HALO RB-10 User manual

HALO RB-10 UV-VIS SPECTROPHOTOMETER
HALO RB-10
Spectrophotometer
Instruction Manual
Please read through this manual carefully and store it
in a safe place.
 Before using the instrument, please read the safety
instructions and precautions carefully
 Keep this manual in a safe place nearby to access

INSTRUCTION MANUAL
Model HALO RB-10 UV-Vis Spectrophotometer
Dynamica Ltd.
Version No: V1.1
Read and keep this manual
· Read the safety instructions carefully and
understand them before using the
equipment.
· Keep this manual at hand for reference.

i
CONTENTS
PREFACE................................................................................................................................. 1
IMPORTANT...................................................................................................... IMPORTANT- 1
Precautions on Electromagnetic
Wave Interference ................................................. IMPORTANT- 1
1. Possible Electromagnetic Wave Interference
Caused by This Instrument ............................. IMPORTANT- 1
2. Possible Electromagnetic Wave Interference
Affecting This Instrument ................................ IMPORTANT- 1
Warranty on Product.............................................. IMPORTANT- 2
Technical Service .................................................. IMPORTANT- 4
Disposal this instrument ........................................ IMPORTANT- 5
Other Precautions.................................................. IMPORTANT- 5
1. Handling of Chemicals and Samples .............. IMPORTANT- 5
2. Notice on Instruction Manuals......................... IMPORTANT- 5
3. Trademark Acknowledgments ........................ IMPORTANT- 5
SAFETY SUMMARY ..........................................................................................SAFETY- 1
PRECAUTIONS ON SAFETY .......................................SAFETY- 1
REMARKS ON SAFETY OF
SPECTROPHOTOMETER............................................SAFETY- 5
WARNING LABEL .........................................................SAFETY- 8
1. INSTALLATION (FOR REFERENCE)............................................................................1- 1
1.1 Unpacking ......................................................................1- 1
1.2 Installation Site ...............................................................1- 1
1.2.1 Installation Conditions ...................................................1- 1
1.2.2 Installation Environment .................................................1- 2
1.3 Check of Contents..........................................................1- 3
1.4 Check of Voltage and Fuse ............................................1- 4
1.5 Side Panel of the HALO RB-10 ......................................1- 5
1.6 Connection of Power Cord and Grounding Wire ............1- 5
1.7 Connection of Printer......................................................1- 6
1.8 Local Remote Switch......................................................1- 7
1.9 LCD Brightness adjustment port ....................................1- 7
1.10 Connecting Accessory to HALO RB-10..........................1- 7
1.11 Connecting HALO RB-10 to PC software.......................1- 7

ii
2. FUNCTION......................................................................................................................2- 1
2.1 Application......................................................................2- 1
2.2 Operating Principle.........................................................2- 2
2.2.1 Optical System ...............................................................2- 2
2.2.2 Signal Processing and Control System ..........................2- 3
2.3 Proper Use of Spectrophotometer..................................2- 3
2.3.1 Selection of Solvent........................................................2- 3
2.3.2 Special Samples.............................................................2- 4
2.4 Functions........................................................................2- 4
2.4.1 Quantitative Analysis......................................................2- 4
2.4.2 Wavelength Scan ...........................................................2- 5
2.4.3 Secondary Processing Functions...................................2- 5
2.4.4 Secondary Processing Functions...................................2- 5
2.4.5 Automatic Calibration and Self Diagnosis Functions......2- 6
2.5 Specification ...................................................................2- 6
3. OPERATION METHOD..................................................................................................3- 1
3.1 Basic Operation..............................................................3- 1
3.1.1 Operating Procedure ......................................................3- 1
3.1.2 Keypad Functions...........................................................3- 1
3.2 Starting Up the Spectrophotometer................................3- 2
3.2.1 Initialization.....................................................................3- 2
3.2.2 Main Menu......................................................................3- 3
3.2.3 Selecting Options from the Main Menu ..........................3- 4
3.3 Photometry .....................................................................3- 5
3.3.1 Using Photometry Mode.................................................3- 5
3.3.2 RATIO Mode ..................................................................3- 10
3.3.3 Using the Concentration Mode.......................................3- 14
3.4 Wavelength Scanning ....................................................3- 21
3.4.1 Wavelength Scan Mode .................................................3- 21
3.4.2 Step by Step Operation ..................................................3- 21
3.4.3 Definition of the Options .................................................3- 22
3.4.4 Baseline Correction ........................................................3- 23
3.4.5 Beginning a Wavelength Scan .......................................3- 24
3.4.6 Post-Run Processing......................................................3- 25
3.4.7 Wavelength Scan Sampling Internal List........................3- 28
3.5 Time Scan ......................................................................3- 29
3.5.1 Using Time Scan Mode ..................................................3- 29
3.5.2 Step by Step Operation ..................................................3- 29
3.5.3 Beginning a Time Scan ..................................................3- 30
3.5.4 Post Run Processing......................................................3- 31
3.5.5 Time Scan Sampling Internal List...................................3- 32
3.6 System Setting ...............................................................3- 33
3.6.1 Using System Mode .......................................................3- 33

iii
3.6.2 Step by Step Operation ..................................................3- 33
4. MAINTENANCE..............................................................................................................4- 1
4.1 Self-diagnosis and Automatic Adjustment......................4- 1
4.2 Confirmation of Specifications........................................4- 1
4.2.1 Wavelength Accuracy.....................................................4- 1
4.2.2 Spectrum BandWidth .....................................................4- 1
4.2.3 Check for Baseline Flatness...........................................4- 1
4.3 Periodical Inspection ......................................................4- 1
4.3.1 Cleaning of Sample Compartment .................................4- 2
4.3.2 Cleaning of Sample Compartment Window ...................4- 3
4.4 Troubleshooting Table....................................................4- 5
5. REPLACEMENT PARTS................................................................................................5- 1
5.1 Consumables and Spare Parts ......................................5- 1
5.2 Parts Replacement.........................................................5- 1
5.2.1 Replacement of Light Source Lamp ...............................5- 1
5.3 Reset the lamp usage time.............................................5- 5
Notice.......................................................................................................................... …….…A

1
PREFACE
Thank you very much for your purchase of Dynamica Model HALO
RB-10 Spectrophotometer.
This instruction manual has been prepared for users of the HALO
RB-10. It describes how to handle the Spectrophotometer.
The Model HALO RB-10 Spectrophotometer is intended for the
purpose of photometric analysis. Do not use it for any other purpose.
The Model HALO RB-10 is designed for use by persons having a
basic knowledge of chemical analysis. Other persons should
operate the instrument in the presence of someone who has such
basic knowledge. Remember that improper use of the instrument,
chemicals or samples would result not only in wrong analytical data
but in consequences adverse to safety.
Before using the instrument, be sure to read through this instruction
manual to enable correct use.
For installation/maintenance of the instrument, read the instruction
manual carefully to attain a full understanding of the instructions. Be
sure to observe cautionary instructions.
Keep this manual handy for easy reference when necessary.
Information contained in this manual is subject to change without
notice for product improvement.
ABOUT THIS MANUAL
Before using the instrument, be sure to read through this
instruction manual. First of all, read “SAFETY SUMMARY”
included at the beginning of this manual for ensuring safety in
operation of the spectrophotometer main unit.

IMPORTANT-1
IMPORTANT
Precautions on Electromagnetic Wave Interference
1. Possible Electromagnetic Wave Interference Caused by This Instrument
When this instrument is used in a residential area or an adjacent
area thereto, it may cause interference to radio and television
reception.
To prevent this, use the specified system connection cables in strict
accordance with the instruction manual. The instrument is designed
to minimize possible electromagnetic wave interference caused by it
if the specified cables are connected properly.
However, there is no guarantee that electromagnetic wave
interference will not be caused by the instrument. If the instrument
does cause interference to radio or television reception, which can
be determined by turning off and on the instrument, the user is
encouraged to try to correct the interference by one or more of the
following measures:
•Increase separation between the instrument and radio/TV
receiver.
•Connect the instrument to an outlet on a circuit different from that
to which the radio/TV receiver is connected.
2. Possible Electromagnetic Wave Interference Affecting This Instrument
If this instrument is used near an intense electromagnetic source,
interference noise may be given to the instrument to incur an
adverse effect on its performance or functionality.
To prevent this, use the specified system connection cables in strict
accordance with the instruction manual. The instrument is designed
to minimize possible electromagnetic wave interference affecting it if
the specified cables are connected properly.
However, there is no guarantee that electromagnetic wave
interference will not occur in this instrument. If the instrument does
incur electromagnetic wave interference, which can be determined
by turning on and off possible sources of electromagnetic wave
interference nearby, the user is encouraged to try to correct the
interference by one or more of the following measures:

IMPORTANT-2
•Reorient the instrument.
•Increase separation between the instrument and possible
sources of electromagnetic wave interference.
•Increase separation between the power cable of the instrument
and possible sources of electromagnetic wave interference.
•Connect the instrument to an outlet on a circuit different from that
to which possible sources of electromagnetic wave interference
are connected.
•Confirm that any other device connected with the instrument is
not affected by electromagnetic wave interference.
Warranty on Product
The Model HALO RB-10 Spectrophotometer is warranted to operate
according to the specifications given in the instruction manual,
provided it is used in accordance with the instructions described in
the manual.
(1) Scope of Warranty
(a) Any parts which prove to be defective in design or
workmanship during the warranty period will be repaired
without charge.
(b) A substitute part may be used for repair, or replacement
with an equivalent product may be made instead of repair.
(c) Such system components as a personal computer and
printer to be updated frequently for improvement may not
be available in original versions at the time of replacement.
(2) Warranty Period
One year from the date of initial installation.
Consumables such as WI and D2lamps are excluded from
warranty.
(3) Availability of Technical Support Service
Technical support service for this instrument is available within
regular working hours on workdays predetermined by us.

IMPORTANT-3
(4) Limitations and Exclusions on Warranty
Note that this warranty is void in the following cases, even if
they occur within the warranty period.
(a) Failure due to operation at a place not meeting the
installation requirements specified by us
(b) Failure due to power supply voltage/frequency other than
specified by us or due to abnormality in power supply
(c) Corrosion or deterioration of the tubing due to impurities
contained in reagent, gas, air or cooling water supplied by
the user
(d) Corrosion of the electric circuits or deterioration of the
optical elements due to highly corrosive atmospheric gas
(e) Failure due to use of hardware, software or spare parts
other than supplied by us
(f) Failure due to improper handling or maintenance by the
user
(g) Failure due to maintenance or repair by a service agent
not approved or authorized by us
(h) After disposal of this instrument, or after its resale without
our approval
(i) Failure due to relocation or transport after initial installation
(j) Failure due to disassembly, modification or relocation not
approved by us
(k) Consumables, and failure of parts that have reached the
end of specified useful life
(l) Failure of parts excluded from the warranty in the
instruction manual or other documents
(m) Failure due to acts of God, including fire, earthquake,
storm, lightning, social disturbance, riot, crime,
insurrection, war (declared or undeclared), radioactive
pollution, contamination with harmful substances, etc.

IMPORTANT-4
(n) Failure of the hardware, or damage to the system software,
application software, data or hard disk due to computer
virus infection
(o) Failure of the personal computer connected with the
instrument, or damage to the system software, application
software, data or hard disk due to power interruption or
momentary power voltage drop caused by lightning or the
like
(p) Failure of the personal computer connected with the
instrument, or damage to the system software, application
software, data or hard disk due to disconnection of main
power to the personal computer without taking the
specified normal shutdown procedure
(5) Disclaimer of Warranty
(a) Any express warranties other than the explicit conditions
indicated in (1) are excluded from this warranty.
Any other implied warranties of merchantability and fitness
for a particular purpose are not included in this warranty.
No liability is assumed for direct or indirect damages
arising out of explicit or implied warranties.
(b) Oral or written information or advice given by our dealers,
distributors, agents or employees without our express
permission shall not create a warranty or in any way
increase the scope of this warranty.
Technical Service
Installation of this instrument shall be carried out by or under
supervision of qualified service personnel of Dynamica Corporation or
its authorized service agent.
Before installation of the instrument, the user shall make
preparations for satisfying the installation requirements in
accordance with the instruction manual.
If relocation of the instrument becomes necessary after initial
installation (delivery), please notify your local sales representative or
nearest service office of Dynamica.

IMPORTANT-5
Disposal this instrument
When you discard equipment, please check and discard a related
statute etc. or ask the service section of Dynamica.
Other Precautions
1. Handling of Chemicals and Samples
(1) The user is responsible for following relevant legal standards
and regulations in handling, storage and discarding of
chemicals and samples used in analytical operations of this
instrument.
(2) Reagents, standard solutions and accuracy-control samples
shall be handled, stored and discarded as instructed by the
respective suppliers.
2. Notice on Instruction Manuals
(1) Information contained in the instruction manuals furnished with
the instrument is subjected to change without notice for product
improvement.
(2) This manual is copyrighted by Dynamica with all rights
reserved.
(3) No part of this manual may be reproduced or transmitted in any
form or by any means without our express written permission.
3. Trademark Acknowledgments
Microsoft, Windows, Microsoft Excel, Microsoft Word and Windows
XP are registered trademarks, trademarks or trade names of
Microsoft Corporation, USA, while other company names and
product names are those of respective companies.

SAFETY-1
PRECAUTIONS ON SAFETY
Before using the Model HALO RB-10 Spectrophotometer, be sure to
read the following safety instructions carefully.
GENERAL SAFETY GUIDELINES
•Follow all the operating procedures provided in this manual.
•Installation and maintenance of the product shall be carried out by
service personnel qualified therefor.
•Be sure to observe the warnings indicated on the product and in
the instruction manual. Failure to do so could result in personal
injury or damage to the product.
•The hazard warnings which appear on the warning labels on the
product or in the manual have one of the following alert headings
consisting of an alert symbol and signal word DANGER,
WARNING, or CAUTION.
DANGER: Indicates an imminently hazardous situation
which, if not avoided, will result in death or
serious injury.
(This warning does not apply to this product.)
WARNING : Indicates a potentially hazardous situation
which, if not avoided, can result in death or
serious injury.
CAUTION : Indicates a hazardous situation which, if not
avoided, will or can result in minor or
moderate injury, or serious damage to the
product.
: Precedes every signal word for hazard
warnings, and appears in safety-related
descriptions in the manual.
SAFETY SUMMARY

SAFETY-2
General Safety Guidelines (Cont’d)
“NOTICE” and “NOTE” are heading words which do not concern
personal safety directly.
NOTICE : Used to indicate an instruction for preventing damage
to the product.
NOTE: Used to indicate an instruction for ensuring correct use of
the product and accurate analysis therewith.
•Do not modify the product, replace parts that are not user-
serviceable, use non-specified parts, nor remove safety devices,
as it could be hazardous.
•Installation at delivery, maintenance and relocation should be
referred to our qualified service personnel.
•Do not perform any operation or action other than described in the
instruction manual. When in doubt, please contact our sales
representative or service office nearest you.
•When using a chemical for analytical operation, be sure to provide
proper ventilation in the laboratory room as per local requirements.
Inadequate ventilation could endanger human health.
•Keep in mind that the hazard warnings in the manual or on the
product cannot cover every possible case, as it is impossible to
predict and evaluate circumstances beforehand.
Be alert and use your common sense.
SAFETY SUMMARY

SAFETY-3
General Safety Guidelines (Cont’d)
This instruction manual contains the following cautious instructions.
DANGER The warning “DANGER” does not apply to this product.
WARNING •Electric Shock in Contact with Dangerous High Voltage
(500 V)
In contact with D2lamp power supply voltage (500 V), you may
receive an electric shock to cause fatal or serious injury.
Before replacing the D2lamp, make sure that the POWER switch
of the spectrophotometer main unit is turned off. (Section 5)
•Electric Shock in Contact with Dangerous Voltage
In contact with power supply voltage, you may receive an electric
shock to cause fatal or serious injury.
Before connecting the power cord, make sure that the POWER
switch of the spectrophotometer main unit is turned off.
(Section 1)
SAFETY SUMMARY

SAFETY-4
General Safety Guidelines (Cont’d)
CAUTION •Burn in Contact with High Temperature
The D2lamp and WI lamp reach a high temperature.
They can burn you if touched.
Before replacing or adjusting the lamp, turn off the POWER switch
and wait until it cools down sufficiently. (Section 5)
•Fatigue on Long Use
Watching the CRT screen in the same posture for a long time
accumulates fatigue in your eyes or body. For long use, take a 10
to 15-minute recess every hour for your health.
•Oxygen Deficiency due to Presence of Nitrogen
(when using Ultra Violet region measurement system)
If nitrogen purging is carried out for long hours in a narrow room
without operating an exhaust duct, the oxygen concentration in
the room will decrease and an oxygen deficiency could result.
Be sure to operate an exhaust duct or else open a window during
the purge for ventilation purposes. (Section 1)
SAFETY SUMMARY

SAFETY-5
Remarks on safety of spectrophotometer
Electricity
(1) Confirm the power supply to the spectrophotometer.
Fluctuation in voltage or noise on the power line would not only
affect the spectrophotometer main unit adversely but also
cause an accident.
(2) Be sure to provide grounding connection along with power
connection. Make sure that the spectrophotometer is grounded
at a grounding resistance of 100 Ωor less.
If grounding is improper, the spectrophotometer is easily
affected by external noise and floating voltage generated in it
endangers physical safety.
(3) High-voltage circuits are used inside the spectrophotometer.
Do not open any other cover than necessary for operation.
Fire
Avoid smoking and using a fire in the vicinity of the
spectrophotometer.
SAFETY SUMMARY

SAFETY-6
Computer Virus
If programs or data has suddenly been destroyed, if an unexpected
operation takes place or if an abnormal display appears on the
screen, your personal computer may have been infiltrated by a
computer virus. The computer virus is a rogue program that secretly
invades a personal computer and operates it willfully while
destroying memorized data.
A program for eliminating the virus is called vaccine program.
There is a possibility of contamination with a computer virus by
downloading through communication a program including a
computer virus, or by using an exchangeable recording medium
such as a floppy disk that contains a computer virus. Also, it is
possible to transmit the virus from one personal computer to another
via communication or recording media. So avoid using a program
or recording medium that might include a virus.
Carry out check using a vaccine program if there is a possibility of
contamination with a virus. But depending on the type of vaccine
program, it may be impossible to eliminate the virus.
It is therefore recommended to back up the contents of the
hard disk beforehand.
Note that the preparation of a vaccine program and the elimination of
a computer virus must be carried out by the user.
Power Failure
A power failure or momentary voltage drop of the power supply due
to lightning, etc. may cause failure of the personal computer used
with the instrument and also damages the system software,
application software and other data.
To avoid such problems, it is recommended to use an AC
uninterruptible power source.
SAFETY SUMMARY

SAFETY-7
Personal Computer (PC)
Do not turn off the PC power supply alone. If the PC power supply is
turned off during access to the hard disk or floppy disk, the PC may
malfunction and the data or software stored in it may be destroyed.
To turn off the PC power supply, be sure to complete the
spectrophotometer control and data processing program (PC
software) before taking the shutdown procedure for the system
software.
SAFETY SUMMARY

SAFETY-8
Warning Labels
The warning labels shown below are attached to the Model HALO RB-10
Spectrophotometer.
SAFETY SUMMARY

1-1
Chapter 1 Installation
1.1 Unpacking
After unpacking the shipping crate, carefully take out the spectrophotometer and
place it on a table.
Since the spectrophotometer weighs about 20 kg, care should be taken when
removing it.
Fig. 1-1 Model HALO RB-10 Spectrophotometer
1.2 Installation site
1.2.1 Installation conditions
(1) Power requirements
Power supply voltage: AC100, 115, 220, 230 or 240V (There must be the
Note: The light source-switching mirror is secured with the sponge rubbe
r
material for transport at shipment from the factory. After using, open the
light source cover and remove the protective sponge from the ligh
t
source-switching mirror.
The shipping crate cannot be claimed for compensation even if it is
damaged during the transportation.
Don’t remove when instrument is turned on.