Dynamica HALO DNAmaster User manual

Model HALO DNAmaster Bio-spectrophotometer
HALO DNAmaster
Bio-spectrophotometer
Operation Manual
Revision : D01-201203

IMPORTANT-1
IMPORTANT
Precautions on Electromagnetic Wave Interference
1. Possible Electromagnetic Wave Interference Caused b This Instrument
When this instrument is used in a residential area or an adjacent area
thereto, it may cause interference to radio and television rece tion.
To revent this, use the s ecified system connection cables in strict
accordance with the instruction manual. The instrument is designed to
minimize ossible electromagnetic wave interference caused by it if the
s ecified cables are connected ro erly.
However, there is no guarantee that electromagnetic wave interference
will not be caused by the instrument. If the instrument does cause
interference to radio or television rece tion, which can be determined by
turning off and on the instrument, the user is encouraged to try to
correct the interference by one or more of the following measures:
• Increase se aration between the instrument and radio/TV receiver.
• Connect the instrument to an outlet on a circuit different from that to
which the radio/TV receiver is connected.
2. Possible Electromagnetic Wave Interference Affecting This Instrument
If this instrument is used near an intense electromagnetic source,
interference noise may be given to the instrument to incur an adverse
effect on its erformance or functionality.
To revent this, use the s ecified system connection cables in strict
accordance with the instruction manual. The instrument is designed to
minimize ossible electromagnetic wave interference affecting it if the
s ecified cables are connected ro erly.
However, there is no guarantee that electromagnetic wave interference
will not occur in this instrument. If the instrument does incur
electromagnetic wave interference, which can be determined by turning
on and off ossible sources of electromagnetic wave interference nearby,
the user is encouraged to try to correct the interference by one or more
of the following measures:

IMPORTANT-2
• Reorient the instrument.
• Increase se aration between the instrument and ossible sources of
electromagnetic wave interference.
• Increase se aration between the ower cable of the instrument and
ossible sources of electromagnetic wave interference.
• Connect the instrument to an outlet on a circuit different from that to
which ossible sources of electromagnetic wave interference are
connected.
• Confirm that any other device connected with the instrument is not
affected by electromagnetic wave interference.
Warrant on Product
The Model HALO DNAmaster Series S ectro hotometer is warranted to
o erate according to the s ecifications given in the instruction manual,
rovided it is used in accordance with the instructions described in the
manual.
(1) Sco e of Warranty
(a) Any arts which rove to be defective in design or
workmanshi during the warranty eriod will be re aired
without charge.
(b) A substitute art may be used for re air, or re lacement with
an equivalent roduct may be made instead of re air.
(c) Such system com onents as a ersonal com uter and rinter
to be u dated frequently for im rovement may not be
available in original versions at the time of re lacement.
(2) Warranty Period
One year from the date of initial installation.
(3) Availability of Technical Su ort Service
Technical su ort service for this instrument is available within
regular working hours on workdays redetermined by us.

IMPORTANT-3
(4) Limitations and Exclusions on Warranty
Note that this warranty is void in the following cases, even if they
occur within the warranty eriod.
(a) Failure due to o eration at a lace not meeting the installation
requirements s ecified by us
(b) Failure due to ower su ly voltage/frequency other than
s ecified by us or due to abnormality in ower su ly
(c) Corrosion or deterioration of the tubing due to im urities
contained in reagent, gas, air or cooling water su lied by the
user
(d) Corrosion of the electric circuits or deterioration of the o tical
elements due to highly corrosive atmos heric gas
(e) Failure due to use of hardware, software or s are arts other
than su lied by us
(f) Failure due to im ro er handling or maintenance by the user
(g) Failure due to maintenance or re air by a service agent not
a roved or authorized by us
(h) After dis osal of this instrument, or after its resale without our
a roval
(i) Failure due to relocation or trans ort after initial installation
(j) Failure due to disassembly, modification or relocation not
a roved by us
(k) Consumables, and failure of arts that have reached the end
of s ecified useful life
(l) Failure of arts excluded from the warranty in the instruction
manual or other documents
(m) Failure due to acts of God, including fire, earthquake, storm,
lightning, social disturbance, riot, crime, insurrection, war
(declared or undeclared), radioactive ollution, contamination
with harmful substances, etc.

IMPORTANT-4
(n) Failure of the hardware, or damage to the system software,
a lication software, data or hard disk due to com uter virus
infection
(o) Failure of the ersonal com uter connected with the
instrument, or damage to the system software, a lication
software, data or hard disk due to ower interru tion or
momentary ower voltage dro caused by lightning or the like
( ) Failure of the ersonal com uter connected with the
instrument, or damage to the system software, a lication
software, data or hard disk due to disconnection of main
ower to the ersonal com uter without taking the s ecified
normal shutdown rocedure
(5) Disclaimer of Warranty
(a) Any ex ress warranties other than the ex licit conditions
indicated in (1) are excluded from this warranty.
Any other im lied warranties of merchantability and fitness
for a articular ur ose are not included in this warranty. No
liability is assumed for direct or indirect damages arising out of
ex licit or im lied warranties.
(b) Oral or written information or advice given by our dealers,
distributors, agents or em loyees without our ex ress
ermission shall not create a warranty or in any way increase
the sco e of this warranty.
Technical Service
Installation of this instrument shall be carried out by or under su ervision of
qualified service ersonnel of Dynamica or its authorized service agent.
Before installation of the instrument, the user shall make re arations
for satisfying the installation requirements in accordance with the
instruction manual.
If relocation of the instrument becomes necessary after initial installation
(delivery), lease notify your local sales re resentative or nearest service
office of Dynamica.

IMPORTANT-5
Disposal this instrument
When you discard equi ment, lease check and discard a related statute
etc. or ask the service section of Dynamica.
Other Precautions
1. Handling of Chemicals and Samples
(1) The user is res onsible for following relevant legal standards and
regulations in handling, storage and discarding of chemicals and
sam les used in analytical o erations of this instrument.
(2) Reagents, standard solutions and accuracy-control sam les shall be
handled, stored and discarded as instructed by the res ective
su liers.
2. Notice on Instruction Manuals
(1) Information contained in the instruction manuals furnished with
the instrument is subjected to change without notice for roduct
im rovement.
(2) This manual is co yrighted by Dynamica with all rights reserved.
(3) No art of this manual may be re roduced or transmitted in any
form or by any means without our ex ress written ermission.
3. Trademark Acknowledgments
Microsoft, Windows, Microsoft Excel, Microsoft Word and Windows XP
are registered trademarks, trademarks or trade names of Microsoft
Cor oration, USA, while other com any names and roduct names are
those of res ective com anies.

SAFETY-1
SAFETY SUMMARY
For our safet please read the following precautions carefull before
using the DNAmaster Spectrophotometer.
General Safety Guidelines
 For safe handling of this roduct, lease follow the instruction rocedure in
the manual for this roduct.
 Pay s ecial attention to follow all the hazard warnings on the roduct and in
the manual. Failure to do so can cause injury to you or damage to the
roduct.
 After installation, lease do not move the equi ment. A vibration might
affect the adjustment of the roduct.
 The hazard warnings, which a ear on the warning labels on the roduct or in
the manual, have one of the following alert headings consisting of an alert
symbol and a signal word, DANGER, WARNING, or CAUTION.
DANGER:
Indicates an imminently hazardous situation that, if
not avoided, will result in death or serious injury.
(It
does not apply to this equipment.)
WARNING: Indicates
a potentially hazardous situation that, if not
avoided, can result in death or series injury.
CAUTION : Indicates a hazardous situation that, if not avoided,
will or can result in minor to moderate injury, or
serious damage to the product.
: The al
ert symbol shown precedes every signal word
for hazard warnings, and appears in safety related
descriptions in the manual.

SAFETY-2
SAFETY SUMMARY (Continued)
General Safety Guidelines (Continued)
In addition, the following “Attention” and “Note” are not directly related to the safety of a erson:
Attention:
It is used to present warnings, which are not directly
related to personal injury hazards. It is used to
indicate prevention against damage to the
equipment.
Note: This is used to indicate instructions that en
able you
to operate the equipment accurately and perform
accurate measurements.

SAFETY-3
SAFETY SUMMARY (Continued)
General Safety Guidelines (Continued)
Before Using
 Before using this roduct, lease make sure you read and understand the instructions.
 Please kee this manual in a safe and easily accessible lace so that you can use it when
necessary.
 Please make sure to use this roduct ro erly and follow the instructions as s ecified in this
manual.
 Please make sure to understand and follow the instructions regarding safety in this manual.
 If you do not follow the instructions in this manual, an inaccurate analysis may result or
bodily injury may occur.
 Because of danger, lease make sure not to modify or alter the roduct, make sure not to
use uns ecified arts, and make sure not to o erate the equi ment by removing/defeating
the safety device(s).
 When using chemicals, lease make sure to ventilate the room well. If there is not enough
ventilation, it may be hazardous to your health.
 Although we have carefully considered the instructions written on the roducts and
manuals, it is ossible for an unex ected event to occur. When o erating the equi ment,
aside from following the instructions, be very cautious.

SAFETY-4
SAFETY SUMMARY (Continued)
General Safety Guidelines (Continued)
Precautions for Installation • Maintenance • Relocation and After Sale Technical
Service
 Before installation, confirm that there are no missing items or standard
accessories. If there is something missing or damaged, or ou have noticed an
problems, please contact our nearest representative.
 Operating the equipment without a standard part can damage the equipment
and cause safet concerns. If that occurs, please follow the instruction of the
installer.
 Installation of this instrument shall be carried out b or under supervisions of
qualified service personnel of D namica or its authorized service agent.
 When relocation of this instrument becomes necessar after initial installation
(deliver ), please notif our local D namica sales representative or nearest
D namica service office. Technical support service for this instrument is available
from s service agent approved or authorized b D namica within regular working
hours or workda s.
 Please do not perform an other operations that are not included in the manual.
If an problem occurs with the equipment, please contact the agent from whom
ou purchased it or the service department of D namica.

SAFETY-5
SAFETY SUMMARY (Continued)
WARNING: Poisoning from Organic Solvent Gas
Handling Organic Solvents
 The organic solvent vapor ma be harmful to our health.
WARNING: Eye Injury from Organic Solvents
Handling Organic Solvents
 Please wear protective glasses when using organic solvents. If the organic solvent
should get into our e e, flush our e e immediatel under running water for at
least 5 minutes while keeping our e elids open. See a ph sician for appropriate
treatment.

SAFETY-6
SAFETY SUMMARY (Continued)
WARNING: Electrical Shocks from Im ro er Grounding
Electrical Shock from Improper Grounding
 When wiring the personal computer, power suppl for the thermostatic cell holder
and the like, please make sure to use the 3-prong wire (with ground) provided.

WARNING: Electrical Shock
Electrical Shock from Contact
 High voltage is used inside of the equipment. Never remove the cover except b a
service person or as instructed in the section for lamp replacement.
 When cleaning the inside of the cell compartment, be sure to turn off the unit.
CAUTION: High Tem erature
Burns from high tem eratures
 The lamp will become ver hot during operation, please make sure that the D2
lamp and the tungsten lamp is cooled off when replacing the lamp.

SAFETY-7
SAFETY SUMMARY (Continued)
CAUTION: Injury from incidental contact with the unit.
Handling the sam le com artment cover
 When ou open and close the sample compartment cover, please be careful not to
get our hand or hair caught. Please assure our safet before operation.
CAUTION: Fatigue due to Prolonged Work
Data Processing
 Viewing the displa in our work can cause e e and ph sical fatigue if ou continue
to work in the same posture for extended periods.
 When working with the displa for a prolonged period, for our health, make sure
to take breaks for 10 to 15 minutes ever hour in order to rest our e es and bod .

SAFETY-8
SAFETY SUMMARY (Continued)
NOTE
Precautions regarding Accuracy and Precision of Measurements
 Do a routine check prior to taking measurements. In order to obtain accurate
measuring results, please perform tests as instructed, following the instructions in
Chapter 3.

SAFETY-9
Electricit
 The voltage for the Spectrophotometer s stem and personal computer must be a
single-phase AC 100V to 200V; Variations in the voltage and noise generated in the
power line will cause adverse effects on the spectrophotometer and ma also cause
accidents.
 Please make sure that grounding is provided together with the power suppl wires,
and make sure that it is connected with a grounding resistance of less than 10 Ω
ΩΩ
Ω.
Defective grounding ma not onl cause lower resistance against noise from the
outside but it can also cause the Mass Spectrometer and personal computer to
generate static electricit , which ma involve the danger of electrical shocks.
 A high voltage circuit is used inside the Spectrophotometer. Do not open the
covers when this circuit is operational because of the danger from electrical shock.
Fire Extinguishers
 Do not smoke or use fire within 3 meters of the Spectrophotometer
 Make sure to keep a fire extinguisher near the Spectrophotometer at all times.
Obtain an ABC Powder extinguisher that can be used for normal fires, oil fires, and
electrical fires.

Installation
Installation conditions
Power requirements
Power su ly voltage: AC100, 115, 220, 230 or 240V
Allowable fluctuation is within ±10% of rated voltage.
Frequency: 50 or 60 Hz
Allowable fluctuation is within ±4% of rated frequency.
Power ca acity: 200VA or more.
A ca acity of more than 500VA may be required for use with other
accessory devices.
Grounding line: Grounding line resistance of 100Ω or less is required.
Installation Environment
(1) O erating tem erature: 5~35 deg C.
In order to erform a measurement under the most stable condition, we recommend
that the instrument is used in an air conditioned room of 20 ~ 25 Deg C.
(2) O erating humidity: 45% ~ 85%.
(3) Storage tem erature: -20~ 60 Deg C.
(4) Atmos heric environment:
a) Free from acid gas or alkaline gas and other gases which may corrode metals
significantly.
b) Free from gases which may dissolve aint such as from organic solvents
( articularly benzene, thinner etc).
(5) Other general notes:
a) Avoid direct sunlight (otherwise o tical erformance might deteriorate or the
housing might become discolored). Avoid installation by a window if at all
ossible.
b) Vibrations or shocks strong enough to be felt by the human body must not be
transmitted to the instrument (otherwise the fine adjustment mechanism might
malfunction).
c) Avoid installation in near heat generating a aratus such as a gas burner, electric
heater or oven in order to revent the mainframe cover being heated beyond 70
Deg C.
d) Avoid installation near instruments which generate a strong electric field (such as
an electric welding machine, high frequency furnace or ole transformer).
e) Avoid a dusty environment (otherwise o tical erformance might deteriorate).
f) The line voltage must be stable and free from a ra id fluctuation (otherwise noise
might increase).
g) Do not frequently turn on and off electric instruments (stirrer, vibrator, etc) which
are connected to the same ower line

DNAmaster Quick Manual
Version 1.0 Page 1
The Instrument
On/Off button
Blue backlight- Standby mode
Green backlight- O erating
Read button
The Read button on the unit can use as an alternative way to take the data during measurement.
Touch screen
DNAmaster’s LCD dis lay is ressure sensing which can be used even with gloves on. For best
erformance, fingerti or stylus is referable.
Touch screen
dis lay
Take
Measurement
on/off button

DNAmaster Quick Manual
Version 1.0 Page 2
Specification
DNAmaster Specifications
Lamp source Long life Xenon
Flash
Lamp
Detector Device CCD
Wavelength range: 200-900nm
Measuring range: 0-4.0 OD
Wavelength accuracy: +/-1nm
Slit width: 4nm
Noise: ~0.005 OD (RMS)
Drift: ~0.005 OD
Photometric Accuracy: +/-0.01 OD
Photometric Repeatability: +/-0.005 OD
Stray light: 0.5%T
DNA detection limit : 20ng/µl
Minimum sample volume with Ultramicro Cell:
(Depends on the cap used) 0.5µl
Start up melodies Selectable from 7
types and mute
Energy save mode Yes
Memory storage Internal or SD card
Data storage number 40,000 (internal)
Unlimited (external)
Manual program storage number 10 for each function
Dimension W x D x H (mm)
300 x 300 x 115
300 x 300 x 155 (TC
model)
Weight (kg) 3.5kg
4.5kg (TC model)
Power Requirement 110 - 220 V, 50/60Hz

DNAmaster Quick Manual
Version 1.0 Page 3
The Displa Screen
Main menu
Touch the icon for go to next screen
DNA/RNA: Nuclei acid analysis
DNA/RNA DYE: Dye incor oration
PROTEIN: Protein determination
PROTEIN UV: Protein UV & UV dye
method
OD600: Bacterial cell culture
measurement
SPECTRO: Traditional s ectro hotometer
function
FILE: Saved method and data
SETTINGS: User arameter setting

DNAmaster Quick Manual
Version 1.0 Page 4
DNA/RNA measurement
DNA/RNA
Select measurement from dsDNA,
ssDNA, RNA, Oligonucleotides
Press
1. SET for detail setting
2. LOAD for reload saved arameter
3. ZERO for blank or reference sam le
before measurement
4. READ for measurement
5. PRN for result rinting
Note: Press ZERO key after new setting, this
will be used for all subsequent samples
SET
Parameter setting
In ut
1. Factor
2. 320nm background correction on/off,
3. Pathlength
4. Dilution Factor
5. Unit
6. Sam le Name
Press OK to next ste or Save to store the
arameters
Select between
different sam le
number