Faster ASEPTIC Operating instructions

00254EN – Rev. 01 – 11/2012 – ORIGINAL INSTRUCTION
Commercial office:
Via Merendi, 22
20010 Cornaredo (MI)
Tel. [phone]
Fax. [phone]
E-mail: [e-mail]
OPERATING AND
MAINTENANCE MANUAL
ASEPTIC

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CONTENTS
1 GENERAL 3
2 INSTALLATION 4
2.A INSTRUCTIONS AND CHECKS ON DELIVERY 4
2.B INSTALLATION REQUIREMENTS 4
2.C ELECTRIC CONNECTIONS AND INSTALLATION OF THE WORK SURFACE 5
2.D POSITIONING OF THE ISOLATOR ON THE SUPPORTING TABLE 5
3 OPERATION PRINCIPLES 7
4 OPERATION 8
4.A SYSTEM AND PERFORMANCES CONTROLS 8
4.B REMOTE SIGNALS 8
4.C SYMBOLS OF THE CONTROL PANEL 9
4.D MANAGEMENT AND PROGRAMMING 12
4.E DISPOSAL OF WASTES AND CONTAMINATED MATERIALS 17
4.F ERGONOMICS 18
5 LIMITATIONS 19
6 OPERATING PROCEDURES 20
6.A PRELIMINARY CHECKS 20
6.B SWITCHING ON the ASEPTIC isolator 20
6.C SWITCHING OFF THE ASEPTIC ISOLATOR 20
6.D How introduce material 21
7 MAINTENANCE 22
7.A INSTRUCTIONS FOR DAILY CLEANING OF ISOLATORS (by users) 22
7.B CLEANING OF THE FRONT GLASS 22
7.C INSTRUCTIONS FOR THE STERILIZATION WITH CLARUS L-2 23
7.D INSTRUCTIONS FOR THE STERILIZATION WITH STERIS VHP 24
7.E MOTORS EARTH CONNECTION 25
7.F HEPA FILTERS (by technical assistance personnel) 26
7.G REPLACEMENT OF MOTOR-FANS (by technical assistance personnel) 30
7.H REPLACEMENT OF FLUORESCENT LAMPS (by technical assistance personnel) 32
7.I SPARE PARTS LIST 33
8 MONITORING SYSTEM 34
9 TROUBLESHOOTING - Probable causes of malfunctions 35
10 TECHNICAL SPECIFICATIONS 36
11 TRANSPORT, PACKING and STORAGE INSTRUCTIONS 37
12 ADDITIONAL INFORMATION 39
12.A GUARANTEE 39
12.B ADDRESS FOR TECHNICAL ASSISTANCE (for the distributor) 39
13 TESTS PERFORMED ACCORDING TO EN 14644-3 STANDARD 40
13.A MEASUREMENT OF LAMINAR AIRFLOW VELOCITY 40
13.B MEASUREMENT OF THE INTERNAL PRESSURE 41
13.C MEASUREMENT OF THE NOISE LEVEL 41
13.D MEASUREMENT OF LIGHTING 41
13.E MEASUREMENT OF VIBRATION 41
13.F CONTROL OF ALARMS 42
13.G DEHS TEST FOR HEPA FILTERS 43
13.H Drawings AND DIAGRAMS 44
13.I DIAGRAM FOR MAINTENANCE OPERATIONS 45
13.J FRONTAL DIAGRAM 47
13.K SIDE DIAGRAM 48
14 SUPPORT TABLE 49
15 ASSEMBY OF THE ISOLATOR ON THE SUPPORTING STAND 50
16 SENSORS LIST 51
17 WIRING DIAGRAM: ASEPTIC EQUIPPED WITH CLARUS L-2 52
18 WIRING DIAGRAM: ASEPTIC EQUIPPED WITH STERIS VHP 60
19 DECLARATION OF CONFORMITY 68

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1 GENERAL
Vertical laminar airflow isolators with positive pressure within the working area and physical separation
between operator and product (gloves), the ASEPTIC isolators are principally designed to protect the material
to be manipulated from contamination coming from environment.
The filtered and sterile air passing through the main HEPA filter ensures optimum airflow laminarity on the
work surface. Air cleanliness is also better than ISO 5 @ 0.3 and 0.5 µm particles according to ISO EN
14644-1 standard.
The ASEPTIC isolators are especially suitable for applications such as:
-Sterile manipulation
-Sterility testing
-Microbiology
-Preparation of culture media
-Preparation of ophtalmic solutions
-TPN Preparation
The performances of the isolators are detailed in the TESTING CERTIFICATE.
Furthermore ASEPTIC isolators fulfil the harmonized standards EN 61010-1 as well as EN 61326 according
to the applicable European directives regarding the CE marking.
ASEPTIC isolators comply with the above-mentioned standards ONLY if the instruments connected to the
electrical socket positioned inside the work chamber are "CE" marked or in any case it meets the above
mentioned standards aiming to avoid any electromagnetic interference.
All Faster’s isolators are provided with high insertion loss filters.
Faster s.r.l. cannot be held responsible for malfunctions, damage to people or property due to non-
compliance, poor or no maintenance, or improper use of the isolator.

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2 INSTALLATION
2.A INSTRUCTIONS AND CHECKS ON DELIVERY
Considering the critical nature of the use of the ASEPTIC isolator and the need to keep it in optimum
condition, installation is very important.
ASEPTIC isolators are positioned on a pallet wrapped in an extensible film and contained in a package of
multi-layer strapped cardboard or a wooden cage.
After placing the isolator in its site of use, opened the package and removed the extensible film, check that
the equipment has not suffered any dents or scratches due to transport or improper handling of the package.
In case of any further transport, packing and storage by the user after the initial period of use (e.g. change of
laboratory or factory), contact the technical assistance service or the distributor for more accurate and
precise instructions or for assistance by specialized technicians.
ASEPTIC isolators, with or without package, should be always located in a room sheltered from rain.
2.B INSTALLATION REQUIREMENTS
Install the isolator away from drafts and heat sources (radiators, ventilators/convectors), to ensure proper
functioning.
Install the isolator in a well-ventilated room with a low degree of dust.
Place the isolator away from doors and windows, which may cause malfunctions
Place the isolator in places where there is little human traffic.
The door of the room should be in such a position relative to the isolator as to prevent drafts.
Min. temperature 5 °C
Max. temperature: 40 °C
Max. humidity: 80% at 31 °C, linear drop in relative humidity down to 50% relative humidity
at 40°C.
Before connecting the isolator to the mains power supply, check the necessary voltage and power indicated
on the plate near the power cable. The room must be equipped with an earth connection.
The quantity of heat generated by the isolator, is the following:
ASEPTIC 2-4-2
180 W
These heat value must be added to the heat generated by any instrument (e.g. connected to internal socket
or tap) used by the client in the work chamber.
ATTENTION: the installation must be done by technicians authorized by Faster S.r.l. or by the
official distributor.

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2.C ELECTRIC CONNECTIONS AND INSTALLATION OF THE WORK SURFACE
The electrical connection of the ASEPTIC isolator is made by connecting the power cable located on the top
of the right side of the isolator to a suitable power point (see technical table). When the isolator is connected,
the green light on the control panel switches on (see chapter 4D).
If stipulated by local legislation, insert upstream of the power line an automatic protection overload switch
provided with a differential relay, with a rated switching voltage no greater than 30 mA.
The ASEPTIC can be supplied either with pneumatic or electric Air Tight Valve (ATV) to close and seal the
isolator during sterilization phase or pressure decay test.
If a pneumatic valve is supplied then dry and filtered 6-8 bar compressed air has to be connected to the
solenoid valves on the top of the unit. If an electric valve is supplied, then just standard electrical connection
is needed.
For the installation of the work surface, proceed as follows:
- remove the protective paper from the work surface leant against the back of the isolator, taking care not
to scratch its surface,
- open the safety front window by unscrewing the four screws,
- clean the work surface with a damp cloth soaked in alcohol or soapy water or with a commonly available
product designed for stainless steel,
- place the work surface into the work chamber, let the back to slide on the chamber's supporting bases
up to its back wall,
- close the safety front window with the four screws.
2.D POSITIONING OF THE ISOLATOR ON THE SUPPORTING TABLE
ASEPTIC Isolators are supplied with the relevant supporting table, which they have to be fixed to.
After assembling the supporting table (see instructions at Para. 15) put the supporting table on a flat not
sloping floor and be sure that all the parts of the isolator, that can be opened (control board panel and sash)
are locked. Then position the isolator on the table (See instructions at Para. 16).

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Technical Features Table
Description Unit ASEPTIC 2-4-2
Overall Dimensions (L x H x D) mm 2670x1500x880
Useful dimensions (L x H x D) mm 1200x700x600
Weigh Kg 330
Noise level dB (A) <51
Lighting level Lux >1000
Main voltage V 230V AC 2P+T
Frequency Hz 50
Power W 970
Current A 4,9
Electrical class 1
Protection level IP20
Internal outlet (maximum current for all the sockets: 4A) 2P+T 230V 4A
Fluorescent lamps W 2x36

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3 OPERATION PRINCIPLES
The following are the working principles of the Aseptic isolators.
The air (A) is sucked inside the isolator through an H14 HEPA filter by a dedicated fan from the laboratory.
That process creates the positive pressure condition in the working area (B) to protect the products handled.
The the air is pushed by the main fan in laminar flow conditions into the work chamber (B) of the isolator
trough a second H14 HEPA filter. From here, through the perforated work surface, the air passes trough the
channel situated at the rear of the work chamber (C). Part of the air is exhausted (D) through the exhaust
H14 HEPA filters in the passboxes.
B
C
D
A

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4 OPERATION
4.A SYSTEM AND PERFORMANCES CONTROLS
The ASEPTIC isolator is provided with an automatic regulation system to keep constant the unidirectional
down flow air speed and the positive pressure in the work chamber, even with the progressive clogging of the
HEPA filters up to the maximum pressure supported by the motor-fans.
The suction fan provides to keep 100 Pa, positive pressure, within the work chamber while the unidirectional
down flow air velocity, provided by the main fan, is 0.45 m/s.
The soft-touch control panel is microprocessor-controlled with a display showing all relevant data with regard
to the operating functions, the different alarms and the error messages.
When the isolator is running if you open the front glass, this action activates an audible and visual alarm,
which cannot be silenced since there is no protection for operator.
When the isolator is off the front glass can be opened completely by unscrewing the four knobs that fasten
the glass.
To optimize the visibility inside the work chamber, the isolator is provided with an ergonomically angled front
window (approx. 7 degrees sloping as to the vertical)
4.B REMOTE SIGNALS
On the ceiling of the isolator a PVC box contains 3 terminal clamps with the following dry contacts:
A. Ventilation status (ON/OFF)
It is possible to obtain a 12 Vdc output to connect a led light or alternatively a Normally Open voltage free
contact to be connected to an external circuit.
There are two different working possibilities selectable in the software:
1 The signal starts when ventilation is turned ON and stops when ventilation is OFF.
2 The signal is flashing during the start up phase of the ventilation and is ON when the correct air
flow/pressure conditions are reached.
B. Correct air flow conditions
It is possible to obtain a 12 Vdc output to connect a led light or alternatively a Normally Open voltage free
contact to be connected to an external circuit. That signal is closed when air flow rate is in the correct range
and it is open in case of any flow/pressure alarm.
C. External consent
On request with a software selection it is possible to allow ventilation to be turned ON by a remote volt free
contact. When the ventilation is turned ON the isolator stay in stand by (start up) till the remote normally open
contact is closed.
If during normal ventilation the remote contact is open the following message will appear “REMOTE
CONTACT OFF” and the operator has to switch OFF ventilation as soon as possible and then check the
reasons of the failure.

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4.C SYMBOLS OF THE CONTROL PANEL
List and description of all the symbols and controls of the control panel:
12 13 14 2 15 3 4 16
5678910 11 1
1 MAIN SWITCH:
Position "0" in the "0" position, the green light of the mains voltage is on (3); the LCD
displays the model name "ASEPTIC". In this position the operator can
activate only the fluorescent light (7), the optional U.V. lamp (14) and the
power outlet (6) (with plug installed) and can see the data stored in the
microprocessor by pressing the “STATUS” key (2).
Position "I" by pressing "I" the password to enter is requested. When the password is
typed in (press arrow-up key (▲) 5 times, arrow-down key (▼) 4 times) and
SET pressed the green led of the switch lights up and the isolator starts
operating, LAF and suction motor-blowers are powered and first "CHECK
PANEL" then "STAND-BY" appears on the display. The LCD shows the
required time (about 40 seconds) for the laminar flow and the internal
pressure to reach the pre-set values. In addition, an audible alarm will sound
intermittently during this stand-by period, alerting the operator not to start
working yet. When the audible alarm stops and the message "STAND-BY"
disappears from the display, the isolator is ready for use. The LAF velocity
and the internal pressure are displayed.
NOTE: In any case, it is advisable to wait 5 minutes before starting to work.
2 DISPLAY Rear lit liquid crystal "LCD" display composed of 2 lines of 20 characters
each showing the operating parameters and alarms.
3 LINE The green mains light switches on if the unit is connected to the mains and
the line is live.
4 WORKING CONDITION The green LED lights up when the ventilation works correctly.
5 GAS Not activated.
6 SOCKET This supplies voltage; when enabled, the display shows "POWER ON" (total
current for all sockets: 6 Amp).
7 LIGHT This switches on the fluorescent light; when enabled, the display shows
"Light on". Switching on the fluorescent light automatically the optional U.V.
lamp switches off.

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8 UP/DOWN ARROWS Use the arrow keys to scroll the menu, to program changing parameters and
to put in the password. Three passwords are programmed: 1) to start the
isolator – 2) to enter the operator menu – 3) to enter the main menu to
change the data input (allowed only to authorized technical staff – service –
because unsuitable interventions can cause troubles and incorrect operation
of the isolator).
9 ESC ESC key deletes the operation of data input and goes back to the starting
condition.
10 SET SET key lets you enter the different functions or confirm the data input going
back to the upper level.
11 STATUS If pressed in sequence, the following data will appear on the display:
External Temperature: Shows the temperature outside of the isolator; the
LCD will display (for example) "EXT. TEMPERATURE=27°C" This value is
taken by an electron probe installed outside the isolator.
Internal Temperature: Shows the internal temperature of the isolator work
area; the LCD will display (for example) "INT. TEMPERATURE =30°C”. This
value is taken by an electron probe located inside the isolator.
U.V. Lamp Residual Lifetime: Shows the operating time of the U.V. lamp
pre-set by the user with the appropriate keys. The LCD will display (for
example) "U.V. TIME=XXXX h”. When such time is over, the message "U.V.
LIFETIME OVER" will appear on the line below.
F1, F2, F3 and F4 Residual lifetimes of filters: it is the operation time of the
filters installed in the isolator that can be programmed by the user.
The LCD will display (for example)” RES. TIME FILTER 1=XXXX:YY h:min”.
When such time is over, the message “CHECK FILTER (es.) 1”. Will appear
on the line below.
The filters installed in the isolator follow the numbering listed below
TYPE of FILTERS NUMBER
LAF HEPA 1
SUCTION HEPA 2
INLET HEPA (back of the pass boxes) 3
OUTLET HEPA (Top of the pass boxes) 4
Not Applicable 5
LAF Power: it is shown indirectly by the power supply voltage of the main
motor, expressed as percentage of max. load voltage displayed also in
proportion by a bar.
The display shows the notice: "MOT.LAF = XX % " (max.100%).
EXH Power: it is shown indirectly by the power supply voltage of the suction
motor, expressed as percentage of max. load voltage displayed also in
proportion by a bar.
The display shows the notice: "MOT.EXH = XX % " (max.100%).

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Operating Time: Shows the operating time of the isolator from the moment
when the main switch is positioned on "I"
The LCD will display (for example) "WORK TIME=XXXXXh”. This value
cannot be reset.
12 SPEED REDUCTION By pushing the corresponding red key the password (the same of start) is
requested. Once confirmed the password the function is enabled. When it is
enabled, the corresponding red LED lights up and LAF velocity and internal
pressure are about 50% than their nominal values. The light and the gas
electro valve cannot be switched on. If they are on, they switch off
automatically. The following two messages appear alternatively:
>>>ATTENTION<<<
DANGER
And:
REDUCED AIRFLOW
>>>DO NOT WORK<<<
The “SPEED REDUCTION” function can be enabled only with the main
switch in position “I”. Only the power supply (3) can be operating.
13 U.V. TIMER Not activated
14 U.V. Not activated
15 MUTE The red alarm LED lights up when an alarm condition occurs, which is shown
also by the message appearing on the LCD. By pushing "MUTE" the alarm
stops sounding.
16 UP/DOWN ARROWS Not activated

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4.D MANAGEMENT AND PROGRAMMING
Get access to operator menu when the cabinet is in stand-by pressing “ESC” [9] together with “UP arrow” [8] keys (password). The following diagram shows the organization of “OPERATOR MENU”
By pressing “SET” [10] you can either go to the highlighted entry or confirm data entry while by pressing “ESC” [9] you go back to the beginning
STAND-BY
PASSWORD
ESC+UP
OPERATOR MENU
ALARM CLOCK SET UP
OPERATOR MENU
UV PROGRAMMING
OPERATOR MENU
UV RESID. TIME
OPERATOR MENU
LANGUAGE
OPERATOR MENU
SET UP THE CLOCK
OPERATOR MENU
CHANGE PASSWORD
OPERATOR MENU
HISTORY VIEW
ALARM CLOCK SET UP
ENABLE? YES/NO
SET ALARM CLOCK
DATE & TIME
UV LIGHTING LENGTH
DURATION: hh:mm
UV PROGRAMMING
DATE & TIME
OPERATOR MENU
ILTERS RESIDUAL TIME
UV LAMP
SET ……………….
ILTER 1 LI ETIME
SET UP ……………….
ILTER 2 LI ETIME
SET UP ……………….
ILTER 3 LI ETIME
SET UP ……………….
LANGUAGE
ITALIANO
LANGUAGE
ENGLISH
LANGUAGE
RANCAIS
LANGUAGE
DEUTSCH
LANGUAGE
ESPANOL
SET UP THE CLOCK
DATE & TIME
CURRENT PASSWORD
……………….
SET UP PASSWORD
……………….
VERI Y PASSWORD
……………….
OPERATOR MENU
TIMER SET UP
TIMER SET UP
SET UP hh:mm
OPERATOR MENU
REMOTE CONTROL
REMOTE CONTROL
REMOTE ENABLE
REMOTE ENABLE
ENABLE? YES/NO
OPERATOR MENU
VHP MODE
OPERATOR MENU
PRESSURE TEST

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TIMER (countdown):
- use "UP/DOWN arrow" keys [8] to scroll the operator menu
- select “TIMER SET UP.” and press "SET" [10] key; the display will show:
TIMER SET UP
SET UP hh:mm
- input the desired time and press SET [10] to confirm
- press ESC to exit the operator menu
- the display will show alternatively the countdown and the standard information
- when the countdown finish an audible signal will advise the operator. It is possible silence the signal
with the ESC key
To disable the countdown:
- select “TIMER SET UP” and press "SET" [10]; the display shows:
TIMER SET UP
RESET? YES
- press "SET" [10] to confirm
- press “ESC” [9] to exit the operator menu.
ALARM CLOCK:
- use "UP/DOWN arrow" keys [8] to scroll the operator menu
- select “ALARM CLOCK SET UP” and press "SET" [10]; the disply shows:
ALARM CLOCK SET UP
ENABLE? YES
- press SET [10] to confirm and the display shows:
.ALARM CLOCK SET UP
DATE & TIME
- set the date and the time with the arrow keys and confirm pressing SET; when the time previously set
is reached and audible signal will advise the operator. Is possible silence the signal with the ESC key.
To disable this function:
- select “ALARM CLOCK SET UP” and press "SET" [10]; the display shows:
ALARM CLOCK SET UP
ENABLE? NO
- disable the alarm clock choosing “NO” and press "SET" [10] to confirm
- press “ESC” [9] to exit the operator menu.

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UV PROGRAMMING:
- use "UP/DOWN arrow" keys [8] to scroll the operator menu
- select “UV PROGRAMMING” press "SET" [10]; and the display shows:
UV LIGHTING LENGTH
SET UP hh:mm
- input the desired time for the UV cycle and press SET [10] to confirm
- the display shows:
.UV PROGRAMMING
DATE & TIME
- set date and time with the arrow keys and confirm pressing SET. when the time previously set is
reached the UV lamp switches ON, if the requirements to switch ON the UV lamp are not satisfied
(e.g.: glass open) an alarm message will be displayed.
- press “ESC” [9] to exit the operator menu.
U.V. LAMP RESIDUAL LIFETIME:
- use "UP/DOWN arrow" keys [8] to scroll the operator menu
- select “U.V. LAMP RESIDUAL LIFETIME.” and press "SET" [10] key; the display will show:
U.V. LAMP RESIDUAL LIFETIME
set XXXX
- where XXXX shows the number of the hours set for lifetime of the U.V. lamp.
- Use the “up and down arrow” keys to adjust the hours parameter
- Then press the "SET" key [10] to confirm the data and/or go back to previous menu
- To conclude programming, press ESC” [9] key.
FILTERS RESIDUAL LIFETIME:
- use "UP/DOWN arrow" keys [8] to scroll the operator menu
- select “FILTERS RESIDUAL LIFETIME.” and press "SET" [10] key; the display will show:
FILTER 1 RESIDUAL LIFETIME
set XXXX
where XXXX shows the number of the hours set for lifetime of the FILTER 1
- Use the “up and down arrow” keys to adjust the hours parameter
- Then press the "SET" key [10] to confirm the data and pass to filter 2 and so on up to filter 5
- To conclude programming, press ESC” [9] key.

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LANGUAGE SELECTION
- use "UP/DOWN arrow" keys [8] to scroll the operator menu
- select “LANGUAGE” and press "SET" [10] key; the display will show:
LANGUAGE
English
- With the “up and down arrow” keys select the desired language (Italian, English, French, German,
Spanish). Press the “SET” key to confirm and exit the “LANGUAGE” menu.
- Press the “SET” key to exit the Operator Menu and return to the standard display.
- press “ESC” [9] key to go out.
SET UP THE CLOCK
- use "UP/DOWN arrow" keys [8] to scroll the operator menu
- select “SET UP THE CLOCK.” and press "SET" [10] key; the display will show:
SET UP THE CLOCK
set XXXX
- Use the “up and down arrow” keys to change the hours, minutes, days, month, year, weekday
- Then press the "SET" key [10] to confirm the data and/or go back to previous menu
- To conclude programming, press ESC” [9] key.
PASSWORD CHANGE
- use "UP/DOWN arrow" keys [8] to scroll the operator menu
- select “CHANGE PASSWORD” and press "SET" [10] key; the display will show:
CURRENT PASSWORD
PSW:
- digit the present Password then press “SET” key
SET UP PASSWORD
PSW:
- digit the new Password then press “SET” key
CHECK PASSWORD
PSW:
- digit the password again and then press “SET” key to confirm the data and/or go back to previous
menu
- To conclude programming, press ESC” [9] key.

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DISPLAY OF HISTORICAL FILES
- use "UP/DOWN arrow" keys [8] to scroll the operator menu
- select “HISTORY VIEW” and press "SET" [10] key; the display will show:
- use "UP/DOWN arrow" keys to scroll through the list of the possible troubles happened . The list is in
chronological order and contains up to 64 voices
- To conclude programming, press ESC” [9] key.
REMOTE CONTROL
- use "UP/DOWN arrow" keys [8] to scroll the operator menu
- select “REMOTE CONTROL” and press "SET" [10] key; the display will show:
REMOTE CONTROL
REMOTE ENABLE
- press again SET and the display shows the following message:
REMOTE ENABLE
ENABLE? YES/NO
- Choose the desired option and press SET
- press “ESC” [9] to exit the operator menu.
VHP MODE
The VHP mode is used to seal compleately the cabinet when a disinfection by gas (H
2
O
2
or Formaldehyde) is
required.
- use "UP/DOWN arrow" keys [8] to scroll the operator menu
- select “VHP MODE” and press "SET" [10] key;
- Follow the instructions: close hatch panels and press “SET”, when the ATV is closed you can start to
vaporize the sterilization agent.
- At the end of sterilization phase press set, ATV will be open and the exhaust fan will start. Open the
hatch panels to let aeration phase to start.
- After a pre-set time the cabinet will aotumatically switch ON ventilation to end the cycle.
PRESSURE TEST
The pressure test is used to find out the leakage rate of the cabinet enclosure.
- use "UP/DOWN arrow" keys [8] to scroll the operator menu
- select “PRESSURE TEST” and press "SET" [10] key;
- using "UP/DOWN arrow" keys [8] it is possible to scroll through 3 menu: START PRESSURE TEST,
SET TEST PARAMETERS, LAST TEST RESULTS.

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4.E DISPOSAL OF WASTES AND CONTAMINATED MATERIALS
DISPOSAL OF ELECTRIC AND ELETTRONIC DEVICES (AEE)
I NFORMATION FOR E UROPEAN U NION USER
This symbol on the device means that when it needs to be disposed, it must be
handled separately from urban waste.
At the moment of the disposal, contact the dealer, to receive information about the
collect and disposal in accordance with the laws in force in the country.
Appropriate disposal of this product will help to prevent potential negative effects on health and environment
and to promote re-use and / or recycling of materials of the equipment.
The improper disposal of the product by holder involves the application of sanctions in accordance with the
regulations in their own country.
I NFORMATION FOR USERS OUTSIDE THE EUROPEAN UNION
This symbol is valid only in the European Union If you want to dispose this product, contact your local
authorities or dealer and ask for the correct method of disposal.
ATTENTION: Before disposal, the isolator where contaminants and pathogens have been
manipulated, must be sterilized
The fluids of pathogenic material removed from the work surface and the liquid collection tank located under
the table as well as from the HEPA filters replaced during maintenance work are toxic and harmful and must
be submitted to special treatment. For more detailed information on such treatment, see the standards and
regulations in force on the treatment and disposal of biologically/toxic/harmful wastes.
All other materials, which the isolator is made, are recyclable but cannot be disposed as local waste
materials.

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MATERIALS, WHICH THE ISOLATOR IS MADE OF
PARTS OF THE ISOLATOR MATERIALS
External structure AISI 304 stainless steel
Inside work chamber AISI 304 stainless steel
Work surface AISI 316L stainless steel
Motor-fans Galvanized steel
Filters Frame: in aluminium alloy
Filtration media: glass fibre
Protection: aluminium grid/epoxy powder painted
Gasket: polyurethane
Keyboard PE, graphite, circuits in materials compatible with Rohs regulations
Front window Laminated safety glass
Wirings Wires according to Rohs regulations, flame-proof cases in PVC
Cable glands Body/nut in polyamide, inside part in EPDM
4.F ERGONOMICS
This isolator has been designed and manufactured according to the general directions on the ergonomics
provided for by the EN ISO 14738 standard.
Furthermore all maintenance operations are assured to be carried out in safety by merely following the
instructions given in this manual at chapter 7.

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5 LIMITATIONS
PRECAUTIONS for the correct use of the isolator
Listed below are the most important guidelines to be followed and the main substances to be avoided to
ensure the correct use of the ASEPTIC isolator:
NEVER USE chlorine-based substances (e.g. sodium hypochlorite) as they are corrosive for the metal
structure of the isolator, and in particular for stainless steel parts.
When the nature of the work carried out under the isolator changes completely or following an accidental
spilling of dangerous material, STOP working and start again only after having cleaned and decontaminate
the isolator,
DO NOT use ethanol as a sterilizing substance if a heat source is used under the isolator,
DO NOT use cosmetic powders, nail polish, hairspray or cosmetics in general during work,
DO NOT eat, drink or smoke in the work zone,
AVOID substances that release explosive vapors.
In addition, when working with the isolator, AVOID:
The introduction of extraneous material
The introduction of paper or cloth that might clog the holes of the work surface
Working under the isolator if the airflow has not yet been activated, i.e. when the display "STAND-BY"
appears, indicating that the motor-fans are not yet on. After the isolator is switched on, and if the work
requires special sterile conditions, chemical sterilization of the work chamber must be carried out using a
cloth soaked in proper cleaning agent. Then wait for the decontamination to take effect.

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6 OPERATING PROCEDURES
6.A PRELIMINARY CHECKS
Before carrying out any type of work, the following conditions should be verified:
– That the isolator power cable is connected to a power point at a suitable voltage and frequency as per
instructions shown on the label stuck near the electric cable
– That all alarm lights are off
– That the work area inside the isolator is free from materials used during the previous session.
– That the isolator has been sterilized in case of change in the nature of work to be carried out
6.B SWITCHING ON the ASEPTIC isolator
To start the isolator, proceed as indicated below:
1. turn on the light by pushing the button [7]
2. be sure that the frontal window is closed
3. press the main key I/0 [1] (see chapter 4D.) and enter the password to switch on the ventilation. At first
the display shows “CHECK PANEL” and the corresponding control leds of the keyboard light up. Then
the message "STAND-BY" is displayed for about 40 seconds and the hour-counter starts operating
4. after 40 seconds of stand-by, the isolator is ready for work. "STAND-BY" disappears from the screen and
the LAF velocity and work chamber pressure are displayed
5. wait for approx. 5 minutes before starting work
6. during this period of time, introduce into the internal work zone of the isolator only the materials which
are indispensable for the job
All movements made under the laminar air-flow isolator should be parallel to the work surface, taking care to
work in the middle of the table to avoid upstream contamination (for instance, when taking a sample from a
bottle, hold the bottle gently but firmly with one hand, keeping it slightly inclined, and work with the other hand,
so that the air that comes into contact with the hand does not directly contact the inside, but rather the outside
of the bottle).
6.C SWITCHING OFF THE ASEPTIC ISOLATOR
ATTENTION: Be careful that materials in the work area can be contaminated with pathogenic
material. Behave accordingly.
At the end of the work session, proceed as follows:
1. Remove the material from the internal work chamber
2. Clean the work surface and the inside walls of the chamber, as indicated in the "Cleaning Instructions"
section (chapter 7A)
3. Let the laminar air-flow isolator run for 10 minutes after the work is over
4. Turn off the light by pushing the key [7]
5. Press “I/0” [1] key switch and enter the password for switching off the isolator (“up-arrow” for 5 times,
“down-arrow” for 4 times, then press “ENTER”).