Fisherbrand Accu20 User manual

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Accu20
Ultrapure Water System
USER MANUAL

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This Manual Is Applicable to the Following Fisher
Scientific Accu20 Models
15489689 Accu20 Water System 230V
15499689 Accu20 Water System, UV SET 230V

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Table of Contents
1 INTRODUCTION................................................................................................ 4
1.1 Product Features.........................................................................................5
1.2 Main Applications ........................................................................................6
1.3 Specifications ..............................................................................................7
1.4 Operation.....................................................................................................8
1.5 The Control Panel........................................................................................9
2 INSTALLATION ............................................................................................... 10
2.1 Preparation for Installation.........................................................................10
2.2 Items Included...........................................................................................10
2.3 System Structure and Flow Diagrams .......................................................11
2.4 Installation .................................................................................................14
3 SYSTEM START UP AND OPERATION ......................................................... 19
3.1 Check List Prior to System Start Up ..........................................................19
3.2 System Start Up and Time Setting.............................................................20
3.3 Routine Operations....................................................................................26
4 MAINTENANCE............................................................................................... 27
4.1 Replacing the AccuDuo P Pack, U Pack Cartridges and the Final Filter ...28
4.2 Replacing the UV Lamp (For UV Models)..................................................30
4.3 Replacing the RO Membrane....................................................................32
4.4 Replacing the Fuse....................................................................................34
5 BASIC TROUBLESHOOTING......................................................................... 35
6 PARTS AND ORDER INFORMATION............................................................. 36
7 WARRANTY INFORMATION........................................................................... 38

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1 INTRODUCTION
This manual describes in detail about system performance characteristics,
installation, operation, and routine maintenance. Please read this manual
thoroughly for its instruction on installation, use and maintenance. Proper
installation and maintenance guarantee the continuous flow of high
quality pure water.
Please contact us or your local distributor if you encounter any issues
during installation and use. Professional engineers are fully trained to
support you.
Safety Information
WARNING!
To avoid electrical shock, always:
1) Use with a properly grounded electrical outlet of correct voltage and
current handling capacity.
2) Replace fuses with those of the same type and rating.
3) Disconnect from the power supply prior to maintenance and service.
4) Refer servicing to qualified personnel.

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1.1 Product Features
The small footprint Accu20 lab water system is an ideal choice for users
who need up to 20 liters of ultrapure water per day. Quality of ultrapure
product water meets or exceeds ASTM, CLSI, CAP, and ISO Type I water
standards.
This system has the following characteristics:
•The compact system provides an easy way to produce Type I
ultrapure water and RO water on demand directly from tap water.
•The system installation and maintenance are very easy to perform by
a trained lab technician.
•The system is equipped with a Safeguard AccuDuo P Pack cartridge
and a dual-column AccuDuo U Pack ultra-purification cartridge to
ensure optimum quality of the final product water.
•No storage tank is required, which eliminates the risk of potential
contamination and bacteria growth inside a tank.
•The fully automatic controlled and maintained system contains
automatic cleaning cycles and self-maintenance functions for the RO
membrane, which increases the lifetime and efficiency of the RO
membranes as well as the cartridges.
•Automatic hourly system recirculation in the loop prevents bacterial
growth and maintains water purity at all times.
•System status and key quality parameters are clearly displayed on a
large LCD screen for easy monitoring of system performances.

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1.2 Main Applications
Pure water can be used in many areas. Here are some typical
applications.
•HPLC mobile phase preparation
•Preparation of reagent blank solutions
•As sample diluents for GC, HPLC, AA, ICP-MS and other analytical
techniques
•Preparation of buffer and culture media for mammalian cell culture
Preparation of molecular biology reagents, etc.

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1.3 Specifications
Operating Voltage 110 V or 230 V
Power < 150 W
System Dimensions
Width × height x depth
12 × 19 × 20 in
30 × 48 × 51 cm
Water Production Rate at 25Ultrapure water (Type I): 0.5 L/min
RO water: 0.5L/min
RO Rejection Rate > 95%
Resistivity of Ultrapure Water 18.2 MΩ.cm
TOC Ultrapure Water < 10 ppb, or < 5ppb (with a dual
wavelength UV lamp)
Particles in Ultrapure Water
(> 0.2 µm)
< 1 /mL (with a 0.2 µm final filter)
UV lamp (optional) 185/254 nm dual wavelength
Microorganism < 0.1 cfu/mL (with a 0.2 µm final filter)
Pyrogen Content < 0.001 EU/mL (with a final ultrafiltration
filter)

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1.4 Operation
Accu20 water systems produce ultrapure water and RO water directly
from tap water. Tap water is pretreated through a Pretreatment Cartridge
(AccuDuo P Pack) to reduce free chlorine, larger particles and water
hardness. Then most particles, ions and organic compounds are removed
through the RO membrane. RO water flows through an ultraviolet UV light
chamber (optional) to kill bacteria and destroy trace organic pollutants in
water. Then water flows through a polishing cartridge U pack to remove
the last traces of ions, finally, a 0.2 µm filters to the outlet.
After powering-up, press START button to initiate the system. After a
180-second automatic RO flush, the system goes into READY mode.
Press the RO button to start a 60-second cleaning cycle and dispense
RO water. Press the RO button again to stop the dispensing. Press the
UP button to start a 60-second cleaning cycle and dispense ultrapure
water. Press the UP button again to stop the dispensing.

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1.5 The Control Panel
Main features of the control panel are:
•MCU technology is used to measure water conductivity with
automatic temperature compensation to 25
o
C.
•Backlit 12864 LCD displays RO conductivity, UP resistivity,
temperature and system operation status.
•System is menu driven, and displays status of auto-run programs.
Technical Specifications
Measurement Range Channel A (RO): 0 ~ 99.9 µS/cm;
Channel B (UP): 0~18.2 MΩ·cm
Temperature Compensation
Range Automatic temperature compensation of readings
Temperature compensation range: 0 ~ 60
o
C to
25
o
C
Range of temperature
compensation coefficient Channel A: compensation coefficient setting range: 0 ~ 5% /
o
C
Channel B: non-linear temperature
compensation
Display Dot-matrix backlit LCD display
Conductivity (or resistivity)
alarm output Can set output upper limit alarm for conductivity (RO) and lower limit alarm for
resistivity (UP)
Communication Interface
Output Standard RS-232C serial port. System can be connected to devices with RS-232C interface to
export data or print records
Power Supply AC230 V ± 10%, frequency (50 ± 0.5) Hz; or
AC110 V ± 10%, frequency (60 ± 0.5) Hz
Maximum working
temperature for conductivity
meter
60
o
C
UV lamp 185/254nm dual wavelength (optional)

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2 INSTALLATION
2.1 Preparation for Installation
2.1.1 Power Supply
Power supply must be properly grounded
2.1.2 Feed Water
2.1.3Tools Needed (Not Included)
Scissors or a box opener to open packages and cut water tubing.
A wrench to fasten the tubing connection.
2.2 Items Included
Accu20 main system includes the following items:
1) One User Manual
2) Quality Certificate
3) Accessories Pack, including one power cord, one 3-way ball valve
(1/2 inch NPTF INLET&NPTM Outlet, 3/8 inch quick connect ), 2
meters of 3/8 inch (9.53 mm) PE tubing, 2 meters of 1/4 inch (6.35
mm) PE tubing, fittings and one roll of Teflon tape
Water type Municipal water
TDS <500 ppm
Water temperature 5 - 35
o
C
Water pressure 1.0 - 6.0 bar / 15 - 90 psi

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2.3 System Structure and Flow Diagrams
2.3.1 System Front View
Display
Control Panel
（see explanation on
following page）Final Filter

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2.3.2 System Control Panel
Main Buttons:
START: Start system. Push once after system power up to initiate the
system.
RO: Controls RO water dispensing. Press once to dispense RO water
and RO indicator light is on. Press again to stop dispensing.
UP: Controls ultrapure (UP) water dispensing. Press once to dispense
UP water and UP indicator light is on. Press again to stop dispensing.
Indicator Lights:
Power indicator: Above START Button. Turns RED after power is
turned on.
RO indicator: Above RO Button. It turns solid GREEN when dispensing
ultrapure water. If RO water does not meet quality standards, this light
BLINKS.
UP indicator: Above UP Button. It turns solid GREEN when dispensing
ultrapure water. If UP water does not meet quality standards, this light
BLINKS.
Power Indicator
RO Indicator UP Indicator
START Button:
Start/Standby RO Button:
RO water
Dispense
UP Button:
Ultrapure water
Dispense

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2.3.3 System Back Side View
2.3.4 Accu20 External Connection Diagram
In from Tap
RO Drain
TAP IN
DRAIN
9.53mm
inlet tubing Tap water
6.35mm outlet
tubing
DRAIN
TAP IN
Power switch Fuse Socket

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2.3.5 Accu20 Water Flow Diagram

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2.4 Installation
Attention
The connectors of the system are protected by stoppers. DO NOT
attempt to pull the stoppers out from the ports without unlocking the
connector first or use excessive force to pull the stoppers out as this
will damage the connector, rendering it useless.
Please follow the instruction below to remove the stoppers
A. Insert the tweezer to the gap between the stopper and the collet.
B. Pinch the tweezer and push it as the picture indicated to remove the
stoppers.
Collet
(push in)

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2.4.1 Installing AccuDuo P Pack and U Pack purification cartridges
Attention!
These two cartridges work in sequence to ensure high quality of water.
These two packs must be installed in a proper sequence as indicated in
the picture below. Never reverse the positions of these packs.
1) Open the right side panel of the system.
2) Remove blue caps on the new cartridges.
3) Install P Pack to the LEFT side position, and the U Pack to the
RIGHT side.
4) Install P pack to the LEFT side position. Wet the O-rings on the
cartridge with pure water, gently insert the lower end first into the
opening on the system frame, then down to let the cartridge sit into its
slot

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5) Push the upper part of the cartridge through the lock-pin till tight
6) Lock the cartridge with the lock-key to the notch on the look-pin
7) Install the U Pack cartridge to the RIGHT side of the P Pack in the
same procedure as that for P Pack
Insert the lock-key
into the notch on
the lock-pin
Cartridge
lock-pin and
notch location
Lower Pack
Locking Clips Lower Pack Locking Clips

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2.4.2 Connecting the tubing to the tap water
Cut appropriate length from the provided 3/8 inch tubing. Then connect
one end of the tubing to the ball valve, the other end to the TAP IN at the
back of the system.
2.4.3 Connecting the Tubing to the Drain
Cut appropriate length from the provided 1/4 inch tubing. Then connect
one end of the tubing to DRAIN at the back of the system, the other end
into a sink.
Note: DO NOT install the final capsule filter!
The attached final filter will be installed after system initial
start-up and degassing in the next chapter.
End of System Installation
Proceed to "System Start up and Operation"
P Pack U Pack

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3 SYSTEM START UP AND OPERATION
3.1 Check List Prior to System Start Up
Prior to system start up, use the table below to make sure all parts have
been installed and connected, and the quality of tap water meets minimal
requirements.
Check List Results
Water quality
Municipal water: TDS < 500 ppm,
Water temperature: 5 - 45
o
C,
Pressure: 1.0 - 6.0 bar / 15 - 90 psi
If water hardness is greater than 100 ppm, Fisher
Scientific suggest you to use an external water softening
device to protect the RO membrane
Tap Water connected and turned on
P Pack cartridge installed
U Pack cartridge installed
Drain tubing installed and laid to the sink

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3.2 System Start Up and Time Setting
3.2.1 Panel Display
System display panel shows four rows of information:
First row: Time.
XX / XX / XX XX: XX
In the following order: Year / Month / Day then Hours / Minutes
Second row: RO status.
XX.X µS /cm 25
o
C
RO Water conductivity compensated to 25
o
C
Third row: UP status.
XX.X MΩ·cm 25
o
C
Ultrapure (UP) water resistivity compensated to 25
o
C
Fourth row: System status.
It displays system operation information and error messages. All
messages scroll in sequence.
XX/XX/XX XX: XX
RO: XX.X µS/cm 25℃
UP: XX.X M·cm 25℃
Operation Status