GeneAmp PCR System 9700 User manual
GeneAmp®PCR System 9700
0.5mL Sample Block Module
User’s Manual
© Copyright 2006, 2010 Applied Biosystems. All rights reserved.
Information in this document is subject to change without notice. Applied Biosystems assumes no responsibility for any errors that may appear in this
document.
APPLIED BIOSYSTEMS DISCLAIMS ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING
BUT NOT LIMITED TO THOSE OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. IN NO EVENT SHALL APPLIED
BIOSYSTEMS BE LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR
SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING
FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE USE THEREOF.
NOTICE TO PURCHASER:LIMITED LICENSE
The combination of the GeneAmp®PCR System 9700 Sample Block Module with a GeneAmp®PCR System 9700 Base Unit is licensed under patent
claims for thermal cycler apparatus and counterpart patent claims in other countries. No other rights are conveyed expressly, by implication, or by
estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Aplied Biosystems, 850 Lincoln Centre
Drive, Foster City, California 94404, USA.
TRADEMARKS:
AB (Design), Applied Biosystems, and MicroAmp are registered trademarks, Applera and Celera Genomics are trademarks of Applera Corporation or
its subsidiaries in the U.S. and/or certain other countries.
GeneAmp is a registered trademark of Roche Molecular Systems, Inc.
All other trademarks are the sole property of their respective owners.
Part Number 4307808 Rev. D
06/2010
Contents
i
1 0.5-mL Sample Block Module
Safety and Regulatory Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
General Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Electrical Safety Testing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Instrument Labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Operating Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Grounding and Electrical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Voltage Quality . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Input/Output Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Physical Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Pollution Category. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
Instrument Storage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
Communautes Europeennes (CE) Compliance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
FCC Compliance (U.S.). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
Routine Maintenance for Safe Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
Technical Support. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
To Reach Us On the Web. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
Hours for Telephone Technical Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
To Reach Us by Telephone or Fax in North America . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
Documents-on-Demand. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-7
To Reach Us by E-Mail . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8
Regional Offices Sales and Service. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8
About the 0.5-mL Sample Block Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-11
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-11
Diagram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-11
Sample Block. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-12
Heated Cover . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-13
Peltier Heating/ Cooling Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-13
Modules, Accessories, and Disposables. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-14
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-14
Ordering. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-14
Installing the 0.5-mL Sample Block Module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-15
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-15
Installing the Sample Block Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-16
Loading Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-17
Loading Samples: Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-17
Reaction Tube/Frame Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-17
ii
Loading Samples into the Reaction Tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-17
Placing the Reaction Tubes into the Sample Block . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-18
Cleaning the Sample Block Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-19
Cleaning: Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-19
Cleaning Position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-19
Cleaning the Heated Cover. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-19
Cleaning the Sample Block Wells. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-20
Running the Calibration Verification Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-21
Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-21
Equipment Required . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-21
Setting Up the 0.5-mL Probe Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-22
Configuring the System 9700 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-23
Running the Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-24
Evaluating the Results. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-26
Running the Temperature Non-Uniformity Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-27
Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-27
Equipment Required . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-27
Setting Up the 0.5-mL Probe Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-28
Configuring the System 9700 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-29
Running the Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-30
Evaluating the Results. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-33
Running System Performance Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-34
Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-34
Equipment Required . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-34
Running the Rate Test. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-34
Running the Cycle Test. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-36
Data Sheet: Calibration Verification Test. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-38
Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-38
Data Sheet: Temperature Non-Uniformity Test. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-39
Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-39
Index
0.5-mL Sample Block Module 1-1
0.5-mL Sample Block
Module 1
Safety and Regulatory Information
IMPORTANT
Read this section before you install the GeneAmp
®
PCR System 9700 and
the 0.5-mL Sample Block Module.
General Symbols
The following symbols are used on Applied Biosystems instruments.Whenever such
symbols appear on instruments, please observe appropriate safety procedures.
Electrical Symbols
This symbol indicates the on position of the main power switch.
This symbol indicates the off position of the main power switch.
This symbol indicates the on/off position of a push-push main power switch.
This symbol indicates that a terminal may be connected to another
instrument’s signal ground reference.
This is not a protected ground terminal.
This symbol indicates that this is a protected ground terminal that must be
connected to earth ground before any other electrical connections are made
to the instrument.
This symbol indicates that this terminal either receives or delivers alternating
current or voltage.
This symbol indicates that this terminal either receives or delivers alternating
and direct current or voltage.
~
~
1-2 0.5-mL Sample Block Module
Non-electrical Symbols
Electrical Safety
Testing
Routine safety testing of analytical instruments (
e.g.
, high potential voltage testing)
may be required by various safety agencies.
Instrument Labels
The following instrument labels are on the system 9700 with the 0.5-mL Sample Block
Module installed.
This symbol indicates the presence of high voltage and warns the user to
proceed with caution.
This symbol alerts you to consult the manual for further information and to
proceed with caution.
This symbol illustrates a heater hazard.Proceed with caution when working
around these areas to avoid being burned by hot components.
Testing should only be carried out by qualified personnel after seeking
advice from the Applied Biosystems Service Department.
! WARNING !
Disconnect supply cord before opening. Grounding
circuit continuity is vital for safe operation of equipment. Never operate
equipment with grounding conductor disconnected.
AVERTISSEMENT: Debrancher le cordon d’alimentation avant d’ouvrir la
continuite des masses est essentielle.
Pour un fonctionnement sans danger. Ne jamais utiliser l’equipment si le fil
de terre n’est pas raccorde.
\
! WARNING !
Hot Surface. Use care when working around this
area to avoid being burned by hot components.
Attention. Surface chaude.
0.5-mL Sample Block Module 1-3
Operating Precautions
Precautions
The following precautions should be taken whenever you operate the system 9700
with the 0.5-mL Sample Block Module installed.
Note
This instrument is able to withstand transient overvoltage according to Installation
Category II as defined in IEC 1010-1.
General Use
Environment, Humidity, and Temperature
\
CAUTION
The instrument should be used according to the
instructions provided in this manual. If used otherwise, the protection
provided by this instrument may be impaired.
CAUTION
This instrument is designed for indoor use.
CAUTION
Do not operate in a Cold Room or a refrigerated area.
The system 9700 will operate safely when the ambient temperature is
5–40 °C (41–104 °F).The system 9700 will meet performance
specifications when the ambient temperature is 15–30 °C and the
ambient relative humidity is 20–80%.These specifications have been
calculated for altitudes between 0 and 2,000 meters.
CAUTION
This instrument is not designed for operation in an
explosive environment. Do not place the instrument close to
potentially explosive materials or objects.
CAUTION
This instrument is not designed for operation with the
heated cover retracted when running at 4 °C. If the heated cover is
retracted and the instrument runs at 4 °C, water condensation may
be excessive in the sample block area.
1-4 0.5-mL Sample Block Module
Sample Block Module
Grounding and
Electrical Safety
The system 9700 must be grounded for protection against electrical shock.
Refer to the
GeneAmp
®
PCR System 9700 Base Module User’s Manual
(P/N 0993-6247) for more information on electrical safety.
Voltage Quality
Line voltage must be within
±
10% of the nominal value.High or low voltages may have
adverse effects on the electronic components of the instrument. In areas where the
supplied power is subject to fluctuations exceeding these limits, a power line regulator
may be required.
Input/Output
Connections
Refer to the
GeneAmp
®
PCR System 9700 Base Module User’s Manual
(P/N 0993-6247) for information on the input and output connections of this
instrument.
Physical
Specifications
Refer to the
GeneAmp
®
PCR System 9700 Base Module User’s Manual
(P/N 0993-6247) for information on the physical specifications of this instrument.
! WARNING !
To protect yourself against burns, do not open the
heated cover or touch the sample block module when the word Hot
displays on the screen.This indicates a sample block temperature
above 50 °C.
The hot areas for the 0.5-mL Sample Block Module are shown below.
\
CAUTION
To protect your samples and to guarantee the best
temperature uniformity, keep the heated cover closed at all times,
except when loading or unloading samples.
! WARNING !
Do not use an adapter to a two-terminal outlet since
this does not provide positive ground protection.
0.5-mL Sample Block Module 1-5
Pollution Category
The system 9700 will operate safely in environments that contain nonconductive
foreign matter up to Pollution Degree 2 in IEC 1010-1.
Instrument Storage
The system 9700 must be stored at a temperature between -20 °C and 60 °C
(-4 °F and 140 °F) at altitudes ranging from 0–12,000 meters above sea level.
IMPORTANT
The system 9700 is guaranteed to meet performance specifications only
when the ambient temperature is 15–30 °C and the ambient relative humidity is 20–80%
(between altitudes of 0 and 2,000 meters).
Communautes
Europeennes (CE)
Compliance
FCC Compliance
(U.S.)
This product is classified as a digital device used exclusively as industrial,
commercial, or medical test equipment. It is exempt from the technical standards
specified in Part 15 of the FCC Rules and Regulations, based on Section 15.103(c).
Routine
Maintenance for
Safe Operation
Before using any cleaning or decontamination method, except those recommended in
the manual, the user should check with Applied Biosystems to ensure that the
proposed method will not damage the equipment.
Maintain your instrument in good working order.In the event that the instrument has
been subjected to adverse environmental conditions (such as fire, flood, earthquake,
etc.), a service inspection of the instrument should be made to ensure safe operation.
\
All instruments shipped to the European Union (EU; formerly known as the
European Community) have the CE label on the back of the instrument.This
label signifies that these instruments comply with the Electromagnetic
Compatibility and Low Voltage Directives.
1-6 0.5-mL Sample Block Module
Technical Support
To Reach Us on the
Web
Applied Biosystems web site address is:
http://www.appliedbiosystems.com/techsupport
We strongly encourage you to visit our web site for answers to frequently asked
questions, and to learn more about our products.You can also order technical
documents and/or an index of available documents and have them faxed or e-mailed
to you through our site (see the “Documents on Demand” section below).
Hours for Telephone
Technical Support
In the United States and Canada, technical support is available at the following times.
See the “Regional Offices Sales and Service” section below for how to contact local
service representatives outside of the United States and Canada.
To Reach Us by
Telephone or Fax in
North America
Call Technical Support at 1-800-831-6844, and select the appropriate option (below)
for support on the product of your choice at any time during the call. (To open a
service call for other support needs, or in case of an emergency, press
1
after dialing
1-800-831-6844.)
Product Hours
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All Other Products 5:30 a.m. to 5:00 p.m. PacificTime
For Support OnThis Product Dial 1-800-831-6844, and...
ABI P
RISM
®
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ABI P
RISM
®
3100 Genetic Analyzer
BioInformatics (includes BioLIMS
™
,
BioMerge
™
, and SQL GT
™
applications)
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GeneScan
®
applications)
Integrated Thermal Cyclers
Press FAX
8650-638-5891
Press FAX
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Press FAX
23 650-638-5891
Press FAX
24 650-638-5891
0.5-mL Sample Block Module 1-7
Documents on
Demand
Free 24-hour access to Applied Biosystems technical documents, including MSDSs, is
available by fax or e-mail.
You can access Documents on Demand through the internet or by telephone:
PCR and Sequence Detection
FMAT
Peptide and Organic Synthesis
Protein Sequencing
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LC/MS
For Support OnThis Product Dial 1-800-831-6844, and...
Press FAX
5, or call
1-800-762-4001,
and press 1 for
PCR, or 2 for
Sequence
Detection
240-453-4613
Telephone FAX
1-800-899-5858,
and press 1, then
press 6
508-383-7855
Press FAX
31 650-638-5981
Press FAX
32 650-638-5981
Telephone FAX
1-800-542-2369
(U.S. only), or
1-781-271-0045
(Tropix)
781-275-8581
(Tropix)
9:00 a.m. to
5:00 p.m. ET
Telephone FAX
1-800-952-4716 650-638-6223
9:00 a.m. to
5:00 p.m. PT
If you want to
order... Then...
through the
internet Use http://www.appliedbiosystems.com/techsupport
You can search for documents to order using keywords.
Up to five documents can be faxed or e-mailed to you by title.
byphone from the
United States or
Canada
a. Call 1-800-487-6809 from a touch-tone phone. Have your fax
number ready.
b. Press
1
to order an index of available documents and have it faxed
to you.Each document in the index has an ID number. (Use this as
your order number in step “d” below.)
c. Call 1-800-487-6809 from a touch-tone phone a second time.
d. Press
2
to order up to five documents and have them faxed to you.
1-8 0.5-mL Sample Block Module
To Reach Us by
E-Mail
Contact technical support by e-mail for help in the following product areas.
Regional Offices
Sales and Service
If you are outside the United States and Canada, you should contact your local
Applied Biosystems service representative.
by phone from
outsidetheUnited
States and
Canada
a. Dial your international access code, then 1-858-712-0317 from a
touch-tone phone.
Have your complete fax number and country code ready (011
precedes the country code).
b. Press
1
to order an index of available documents and have it faxed
to you.Each document in the index has an ID number.(Use this as
your order number in step “d” below.)
c. Call 1-858-712-0317 from a touch-tone phone a second time.
d. Press
2
to order up to five documents and have them faxed to you.
If you want to
order... Then...
For this product area Use this e-mail address
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Synthesis [email protected]
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Tel: 47 23 12 06 05
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0.5-mL Sample Block Module 1-9
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Tel: 7 095 935 8888
Fax: 7 095 564 8787
Spain (Tres Cantos)
Tel: 34 (0)91 806 1210
Fax: 34 (0)91 806 1206
South Africa (Johannesburg)
Tel: 27 11 478 0411
Fax: 27 11 478 0349
Sweden (Stockholm)
Tel: 46 (0)8 619 4400
Fax: 46 (0)8 619 4401
United Kingdom (Warrington, Cheshire)
Tel: 44 (0)1925 825650
Fax: 44 (0)1925 282502
Switzerland (Rotkreuz)
Tel: 41 (0)41 799 7777
Fax: 41 (0)41 790 0676
South East Europe (Zagreb, Croatia)
Tel: 385 1 34 91 927
Fax: 385 1 34 91 840
Middle Eastern Countries and North Africa
(Monza, Italia)
Tel: 39 (0)39 8389 481
Fax: 39 (0)39 8389 493
Africa (English Speaking) and West Asia
(Fairlands, South Africa)
Tel: 27 11 478 0411
Fax: 27 11 478 0349
All Other Countries Not Listed
(Warrington, UK)
Tel: 44 (0)1925 282481
Fax: 44 (0)1925 282509
Japan
Japan (Hatchobori, Chuo-Ku, Tokyo)
Tel: 81 3 5566 6100
Fax: 81 3 5566 6501
Eastern Asia, China, Oceania
Australia (Scoresby, Victoria)
Tel: 61 3 9730 8600
Fax: 61 3 9730 8799
Malaysia (Petaling Jaya)
Tel: 60 3 758 8268
Fax: 60 3 754 9043
China (Beijing)
Tel: 86 10 6238 1156
Fax: 86 10 6238 1162
Singapore
Tel: 65 896 2168
Fax: 65 896 2147
Europe
1-10 0.5-mL Sample Block Module
Hong Kong
Tel: 852 2756 6928
Fax: 852 2756 6968
Taiwan (Taipei Hsien)
Tel: 886 2 2698 3505
Fax: 886 2 2698 3405
Korea (Seoul)
Tel: 82 2 593 6470/6471
Fax: 82 2 593 6472
Thailand (Bangkok)
Tel: 66 2 719 6405
Fax: 66 2 319 9788
Eastern Asia, China, Oceania
0.5-mL Sample Block Module 1-11
About the 0.5-mL Sample Block Module
Overview
The GeneAmp
®
PCR System 9700 amplifies nucleic acids using the polymerase
chain reaction (PCR) process.The 0.5-mL Sample Block Module attaches to the top
of the GeneAmp®PCR System 9700 Base Module and allows you to perform up to 60
reactions.
You can remove the 0.5-mL Sample Block Module and replace it with another sample
block module as needed. Interchangeability lets you change sample well formats as
well as throughput capacity.
IMPORTANT Interchanging sample block modules may require a firmware change.If the
sample block module is placed on a base module running an earlier software version, the
instrument will not operate correctly.To upgrade the base module software, use the 3.5” floppy
disk that is shipped with the 0.5-mL Sample Block Module. For instructions on upgrading the
software, see the GeneAmp®PCR System 9700 Base Module User’s Manual (P/N 0993-6247)
or visit our web site (www.appliedbiosystems.com/techsupport).
Diagram
0.5-mL Sample
Block Module Heated cover
System 9700
Base Module
Sample block
1-12 0.5-mL Sample Block Module
Sample Block The sample block has 60 wells (10 x 6) for use with GeneAmp®Thin-Walled Tubes
with Flat Caps (0.5-mL volume).
The sample block can operate in one of two modes:
♦Sample control (0.5-mL Mode button)
In this mode, the instrument calculates the temperature of the sample during
cycling.You can enter various reaction volumes and the instrument will
compensate for this (e.g., 20 µL heats and cools faster than 100 µL).In this mode,
the sample block itself overshoots the setpoint temperature to get the sample to
temperature more quickly, but the sample temperature will not overshoot.The
clock starts counting down hold time once the sample is within one degree of the
setpoint temperature.
Note The temperature displayed on the screen is the calculated sample temperature.
The illustration below depicts the profile of the sample control mode.
♦Block control (Block Mode button)
In this mode, the reaction volume and sample temperature are ignored.The
sample block itself goes to the programmed setpoint without overshooting.The
clock starts counting down hold time once the sample block reaches the setpoint
temperature.
Note The temperature displayed on the screen is the block temperature.
0.5-mL Sample Block Module 1-13
Heated Cover The heated cover slides over the sample block and performs two functions:
♦It raises the temperature of the upper part of the tube (the part above the sample
block) above the temperature of the sample mixture.This eliminates
condensation, which may have a negative effect on your chemistry.
♦It puts pressure on the reaction tubes and frame to seat them firmly and precisely
in the sample block.This is important for proper heat transfer.
Peltier Heating/
Cooling Unit The internal Peltier heating/cooling unit is housed in the sample block module.The
sample block module is made of aluminum to provide the optimal thermal transfer
rate.
A Resistive Temperature Device (RTD) sensor in the sample block module provides:
♦Wide temperature range:4–99.9 °C
♦Accuracy: ±0.25 °C from 35–100 °C
♦Heat/cool rate: 1.5 °C per second
♦Temperature uniformity: ±0.5 °C (measured 30 seconds after the clock starts)
♦Long-term stability and high reliability
1-14 0.5-mL Sample Block Module
Modules, Accessories, and Disposables
Overview The modules and accessories listed below are available for the GeneAmp®PCR
System 9700 Base Module.The disposables are for use with the 0.5-mL Sample
Block Module.
Ordering Order modules, accessories, and disposables from Applied Biosystems by part
number. See the tables below.
Modules and Accessories Part Number
96-Well Sample Block Module N805-0251
Dual 384-Well Sample Block Module N805-0400
0.5-mL Sample Block Module 4309131
0.5-mL Sample Block ModuleTemperature Verification Kit 4309924
Disposables Part Number
GeneAmp®0.5-mL Thermal Insulation Frame (box of 8) 4308927
GeneAmp®Thin-Walled Tubes with Flat Caps N801-0737
0.5-mL Sample Block Module 1-15
Installing the 0.5-mL Sample Block Module
Overview A lever behind the sample block module releases it from the GeneAmp®PCR System
9700 Base Module.Diagrams of the top and bottom of the 0.5-mL Sample Block
Module are shown below.
Top view, 0.5-mL Sample Block Module
Bottom view, 0.5-mL Sample Block Module
L
ever
L
ever
1-16 0.5-mL Sample Block Module
Installing the
Sample Block
Module
To install the sample block module into the GeneAmp®PCR System 9700 Base
Module:
Note If the sample block module is not seated correctly, the instrument cannot be turned on.
Step Action
1Pull the lever out from the sample block module.
2Place the sample block module onto the base module, then push the sample
block module back to seat the electrical connections.
3Push the lever into the base module to secure the sample block module.
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