Gilson PIPETMAN P Series User manual

EN ENGLISH User's Guide
PIPETMAN®P
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2
PIPETMAN®P
1  INTRODUCTION
The Pipetting Standard – Gilson's PIPETMAN P is
designed and manufactured to provide you with a
range of robust, accurate and precise pipettes. The
PIPETMAN P is a fully adjustable, air-displacement
pipette with the selected volume shown on a digital
indicator (volumeter).
Eight models cover a range from 0.2 µL to 10 mL,
for many applications:
P2 and P10: measurement and transfer of micro-
volumes, DNA sequencing and enzyme-assays.
P20, P100, P200, P1000: measurement and
transfer of general aqueous solutions, acids and
bases.
Contents Page
1  INTRODUCTION 2
2  PARTS CHECK LIST 3
3  DESCRIPTION 3
4  SETTING THEVOLUME 4
5  PIPETTING 5
6  GENERAL GUIDELINES FOR GOOD PIPETTING 8
7  ACCESSORIES 9
8  GLP FEATURES 10
9  TROUBLESHOOTING 10
10  LEAKTEST 12
11  MAINTENANCE 13
12  CLEANING AND DECONTAMINATION 16
13  SPECIFICATIONS 18
14  SPARE PARTS 20
EC DECLARATION OF CONFORMITY 22


3
P5000,P10ml:measurementandtransferoflarge
volumes.
2  PARTS CHECK LIST
Justtakeamomenttoverifythatthefollowingitems
are present:
- PIPETMAN P,
- User's Guide,
- Safety bag,
- Certificate of conformity (including bar-code
sticker).
3  DESCRIPTION
Please refer to the following chapters for a full description
of the dierent parts and functions of the pipette.
of the dierent parts and functions of the pipette.
Color-coded
push-button
Thumbwheel
Tip-ejector button
Body or handle
Connecting nut
DIAMOND tip
Tip-holder
Tip-ejector

4
PIPETMAN®P
4  SETTING THE VOLUME
The volume of liquid to be
aspiratedissetusingthevol-
umeter.Thedialsarecolored
eitherblackorredtoindicate
the position of the decimal
point, depending on the
model (see examples).
The volume is set by turn-
ing the thumbwheel or the
push-button. The push-
button makes it easier and
quicker to set volumes,
especially when wearing
gloves. The thumbwheel
maybeturnedtoslowlyreach
the required setting.
Toobtainmaximumaccuracy
when setting the volume,
proceed as follows:
when decreasing the
volume setting, slowly
reach the required
setting, making sure not
to overshoot the mark.
when increasing the volume setting, pass the
required value by 1/3 of a turn and then slowly
decrease to reach the volume, making sure not
to overshoot the mark.
1
2
5
P2
1.25 µL
0
7
5
P10
7.5 µL
1
2
5
P20
12.5 µL
P100
0
7
5
75 µL
P200
1
2
5
125 µL
P1000
0
7
5
0.75 mL
P5000
1
2
5
1.25 mL
P10ml
0
7
5
7.5 mL
Model Color of volumeter numbers
Black Red Increment
P2 µL 0.01 µL 0.002 µL
P10 to P20 µL 0.1 µL 0.02 µL
P100-P200 µL - 0.2 µL
P1000-P5000 0.01 mL mL 0.002 mL
P10mL mL 0.1 mL 0.02 mL
Push-button
Thumbwheeel

5
5  PIPETTING
For optimum performance, use of Gilson's
DIAMOND Tips with your PIPETMAN P is strongly
recommended. DIAMOND Tips, made from pure
polypropylene have the Gilson logo engraved on
their collar, ensuring that you have a genuine Gilson
product.Plastictipsareforasingleapplication–they
must not be cleaned for reuse.
However, PIPETMAN P can also be used with the
main tip brands.
Fitting the tips
To fitanewGilsonDIAMONDTip,pushthetip-holder
into the tip using a slight twisting motion to ensure
a firm and airtight seal.
FortheP2andP10models,adual-positionadapter(plastic)
isrequiredto tDL10 tips (long tips) orD10 tips (short tips).
The metallic rod of the tip-ejector is shaped so that the
adapter may be clipped to it in either position.
P2 and P10 models are delivered with the adapter
in place, positioned in the longer slot, ready to use
DL10 tips. When D10 tips (which are shorter) are
used,theadaptermustberepositionedintheshorter
slot as follows:
Pull the adapter down from the metallic rod.
Turn the adapter through 180°C.
Refit the adapter so that the end of the metallic
rod engages the shorter slot of the adapter.
Finally, check that the “dimple” on the metallic
rod is engaged in the corresponding hole on
the adapter.

6
PIPETMAN®P
Dual-position adapter for P2 and P10
with D10
with DL10
with D10
Pre-rinse the tips
Some liquids (e.g. protein-containing solutions and
organic solvents) can leave a film of liquid on the
insidewallofthetip;pre-rinsethetiptominimizeany
errors that may be related to this phenomenon.
Pre-rinsing consists of aspirating the first volume
of liquid and then dispensing it back into the same
vessel (or to waste). Subsequent volumes that you
pipette will have levels of accuracy and precision
within specifications.
Aspirate
Press the push-button to the first stop (this
corresponds to the set volume of liquid).
Hold the pipette vertically and immerse the tip
intheliquid(seeimmersiondepthtable,page8).
Releasethepush-buttonslowlyandsmoothly(to
topposition)toaspiratethesetvolumeofliquid.
Wait one second (time depends on model, see
table) ; then withdraw the pipette-tip from the
liquid. You may wipe any droplets away from
the outside of the tip using a medical wipe,
howeverifyoudo so take care toavoid touching
the tip’s orifice.

7
First stop
Second
stop
Dispense
Place the end of the tip against the inside wall of
the recipient vessel (at an angle of 10° to 40°).
Press the push-button slowly and smoothly to
the first stop.
Wait for at least a second, then press the push-
button to the second stop to expel any residual
liquid from the tip. Keep the push-button
pressed fully down and (while removing the
pipette) draw the tip along the inside surface
of the vessel.
Release the push-button,smoothly.Ejectthetip
by pressing firmly on the tip-ejector button.

8
PIPETMAN®P
6  GENERALGUIDELINES FOR GOOD
PIPETTING
Makesurethatyou
operate the push-
button slowly and
smoothly.
When aspirating,
keep the tip at a
constant depth
below the surface
of the liquid (refer
to the table).
Changethetipbeforeaspiratingadifferentliquid,
sample, or reagent.
Change the tip if a droplet remains at the end of
the tip from the previous pipetting operation.
Eachnewtipshouldbepre-rinsedwiththeliquid
to be pipetted.
Liquid should never enter the tip-holder; to
prevent this:
- press and release the push-button slowly and
smoothly,
- never turn the pipette upside down,
- never lay the pipette on its side when there is
liquid in the tip.
If you use the same tip with a higher volume,
pre-rinse the tip.
For volatile solvents you should saturate the
air-cushion of your pipette by aspirating and
dispensing the solvent repeatedly before
aspirating the sample.
When pipetting liquids with temperatures
different to the ambient temperature, pre-rinse
the tip several times before use.
You may remove the tip-ejector (see Chapter 11 -
Maintenance) to aspirate from very narrow tubes.
Table - Immersion Depth
and Wait Time
Model Immersion Wait Time
Depth (mm) (seconds)
P2 1 1
P10 1 1
P20 2-3 1
P100 2-4 1
P200 2-4 1
P1000 2-4 2-3
P5000 3-6 4-5
P10mL 5-7 4-5

9
After pipetting acids or other corrosive liquids
thatemitvapors,removethetip-holder,rinsethe
piston, O-ring and seal with distilled water. For
the model P1000, by using a specific tip holder
equipped with a filter, you can increase the life-
time of the piston (see Chapter 7 - Accessories).
Do not pipette liquids having temperatures
above70°Corbelow4°C.Thepipettecanbeused
between+4°Cand+40°Cbutthespecifications
may vary according to the temperature (refer to
the ISO 8655-2 standard for conditions of use).
7  ACCESSORIES
To make pipetting more comfortable and more
secure, Gilson has developed several accessories:
Toavoidthepossibilityofliquidrunningbackinto
the pipette, store the pipette vertically.
Toidentifyorpersonalizeyourpipette,COLORIS™
clips are available:
WithTHEJIMMY™,handsfreemicrotubeopener,
you can open both snap-cap and screw-cap
microtubes.
To protect the piston when pipetting corrosive
liquids,youcanuseaspecifictipholderandfilter
for the model P1000:
CARROUSEL™ Pipette stand (7 pipettes) F161401
SINGLE™ pipette holder F161406
TRIO™ stand (3 pipettes) F161405
COLORIS™ clips (mixed colors set of 10) F161301
THE JIMMY™ (set of 3) F144983
Corrosion protection kit (tip holder + a bag of lters) F144570

10
PIPETMAN®P
Before returning any pipette, ensure
that it is completely free of chemical,
biological, or radioactive contamina-
tion.RefertoChapter12-Cleaningand
Decontamination.Afterdecontamina-
tion, use the safety bag provided with
the pipette to return it to your local
Gilson authorized Service Center.
8  GLP FEATURES
The Serial Number is
engravedonthebodyof
the pipette. It provides
unique identification
of your pipette and the
date of manufacture.
Ex: CG 12326
The Bar Code on the
box and the certificate
of conformity provide
traceability of your
pipette.
9  TROUBLESHOOTING
A quick inspection of the pipette may help you to
detect a problem.
You may download from the Gilson website (www.gilson.
com) the “2 minute inspection”, which shows how to
perform a quick diagnosis of your pipette.
CG12326