Grant-bio PCMT User manual

Thermo-shaker
with cooling
PCMT
Operating instructions

Thermo-shaker PCMT Version 1.01 - January 2013
Page 1 Operating Instructions
Contents
1 Safety
2 General Information
3 Getting Started
4 Operation of PCMT
5 Calibration
6 Specifications
7 Guarantee and service

Thermo-shaker PCMT Version 1.01 - January 2013
Page 2 Operating Instructions
1. Safety
The following symbols mean:
Caution: Make sure you have fully read and understood the present Manual
before using the equipment. Please pay special attention to sections
marked by this symbol.
Caution: Surfaces can become hot during use!
GENERAL SAFETY
Use only as specified in the operating instructions provided. The unit should not be used if it
has been dropped or damaged.
The unit must be stored and transported in a horizontal position (see package label).
After transport or storage allow the unit to reach room temperature (2-3 hrs) before
connecting to the mains. Use only standard quality tubes.
Before using any cleaning or decontamination method except those recommended by the
manufacturer, check with the manufacturer that the proposed method will not damage the
equipment.
Do not make modifications to the design of the unit.
ELECTRICAL SAFETY
Connect only to the mains with a voltage corresponding to that on the serial number label.
Use only the external power supply unit provided with this product.
Ensure that the mains switch and external power supply are easily accessible during use.
Do not plug the unit into the mains outlet without grounding, and do not use extension lead
without grounding.
Before moving the unit, disconnect it from the mains outlet.
To turn off the unit, disconnect the external power supply from the mains outlet.
If liquid is spilt inside the unit, disconnect it from the external power supply and have it
checked by a qualified repair and maintenance technician.
DURING OPERATION
Do not leave the operating unit unattended.
Do not impede the platform motion.
Do not operate the unit in environments with aggressive or explosive chemical mixtures.
Do not operate the unit if it is faulty or been incorrectly installed.

Thermo-shaker PCMT Version 1.01 - January 2013
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For indoor use only.
Intended for laboratory use only.
Do not check the temperature by touch. Use a thermometer.
BIOLOGICAL SAFETY
It is the user's responsibility to carry out appropriate decontamination if hazardous material
is spilt on or inside the equipment.

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2. General Information
Thermo-Shaker PCMT is an ideal instrument for intensive mixing of samples with heating or cooling.
Heating (up to +100°C), cooling (up to 15°C below room temperature - RT) and mixing modes can be
used both simultaneously and independently i.e. the unit is three products in one:1)ashaker,2)a
thermostatand3)athermo-shaker.
Features of the PCMT:
•Quick to reach the specified mixing speed and set temperature.
•Stability of maintaining the set temperature in a wide range throughout the Thermo-shaker’sblock
surface;
•Withthehelpofthetemperaturecalibrationfunction,theusercancalibratethe unit approx. ±6% of
the selected temperature to compensate for differences in thethermalbehavioroftubesfrom
differentmanufacturers;
•LCD display indicates preset and current values of temperature, speed and timeofoperation;
•Quietmotoroperation,compactsize,prolongedservicelife.
Cooling system–Peltierelements.
Heating system–flatheatingelement(12V).
Mixingtype–orbital.
PCMT isapplicablein:
•DNAanalysisforDNAextractionandfurthersamplepreparation;
•Biochemistryforstudiesofenzymaticreactionsandprocesses;
•Metaboliteextractionfromcellmaterials.
Therearefiveheatingandcoolingblocksavailable,includingablockwithaplastic lidforPCR-plates.
Allblocksare interchangeable andcan be quickly and easilyinstalled.

Thermo-shaker PCMT Version 1.01 - January 2013
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3. Getting started
3.1 Unpacking
Remove packing materials carefully, and retain for future shipment or storage of the unit.
Examine the unit carefully for any damage incurred during transit. The warranty doesnot
coverin-transitdamage.
Attention! Automatic balancing system in this product produces light metal-like noise when moving
the unit which is likely to be heard during unpacking. It is normal occurrence and does not
indicate a fault.
3.2 The Thermo-shaker PCMT set includes:
•Thermo-shaker PCMT ....................................................................................1 piece
•Microtube block ...............................................................................................1 piece
•Spare rubber belt ..........................................................................................2 pieces
•External Power Supply.....................................................................................1 piece
•Operating instructions; CE Certificate...............................................................1 copy
3.3 Optional blocks
•HC32 block...................................for 20x0.2 ml microtubes + 12x1.5 ml microtubes
•HC24N block.......................................................................for 24x1.5 ml microtubes
•HC18 block .....................................................for 20x0.5 ml + 12x1.5 ml microtubes
•HC24 Block.........................................................................for 24x2.0 ml microtubes
•HC96 Block..............................for 96-well PCR microplate (semi-skirted, unskirted)
3.4 Set up:
• Place the unit upon even horizontal non-flammable surface away from any flammable
materials, remove protective film display.
• To provide optimum ventilation ensure clearance around the device - 20 cm on side
faces.
• Plug the external power supply into the socket at the rear side of the unit and position
the unit so that there is easy access to the power switch and external power supply.
3.5 Installing blocks (if block is not installed at the moment of delivery)! Disconnect from the
mains!).
Connect the plug to the contact terminal on the underside of the block. Align the block
so that the warning label is facing the front of the unit and secure with the four knurled
screws.
3.6 Changing blocks
Disconnect the external power supply from the
mains. Remove the four knurled screws,
disconnect the plug. Select the new block.
Connect the plug.

Thermo-shaker PCMT Version 1.01 - January 2013
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Align the block so that the warning label is
facing the front of the unit and secure with the
four knurled screws.
3.7 Note! When installing the HC96 block, drive one by one 4 screws for several turns and fix
them with a hex-key (included in the HC-96 thermo-block set).
Fig.1 Thermo-block connection
3.8 Changing Blocks
Disconnect the external power supply unit from the device.
Remove the four knurled screws (fig.2/1); disconnect the plug (fig.1/1).
Select the new block and install it as point 3.5
Fig.2 Thermo-block set up

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4. Operation of PCMT
Recommendations during operation
•Please check that the tubes/microwell plates are thermo resistant. Don't heat the
tubes/microwell plates over the melting point of the material they are made of (use thermo
resistant polypropylene tubes). Remember that thin-walled tubes have a higher thermo
conductingfactor.
• Under high temperatures (> 85°C) tube caps can open, causing evaporation of the sample or a
potential health risk when working with infected material. To prevent such occurrences it is
recommendedtousetubeswithcaplockofSafe-Lock® t y p e .
• Forefficientmixingitisrecommendedtofilltesttubesupto75%ofthetube specifiedvolume.
4.1 Connect the external power supply to the grounded mains and switch ON the power switch
located on the rearpaneloftheunit.
4.2 The display will turn on with the upper line (Set) showing time, RPM and temperature set
earlier and the lower line (Actual) showing current readings of the same parameters
(thermo block temperature °C, which automatically starts rising according to the temperature
set in the upper line). The time to reach the set temperature will depend on the initial
temperature.
Setting the PCMT
Use the readings in the upper line of the display (Set), while setting the required parameters.
Fig.3 Control Panel
Timeset(TIME)
4.3 With the help of “▲” and “▼“ keys Fig. 3/1 set the required working time interval in hours and
minutes (increment - 1 min). If the key is pressed for more than 3 seconds, the increment
becomes bigger.
Speed set (RPM)
4.4 With the help of “▲” and “▼“ keys Fig. 3/2 set the required speed (increment 10 RPM). If the
key is pressed for more than 3 seconds, the increment becomes bigger.
Temperature set (T, ºC)
4.5 With the help of “▲” and “▼“ keys Fig. 3/3 set the necessary temperature (increment
0.1ºC). If the button is pressed for more than 3 seconds, the increment becomes bigger.
1 2 3 4
00:00 1000 35.0
STOP 000 35.0
hr:min
5

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The set parameters can also be changed during operation.
Caution! The timer ending does not stop the heating. Heating can only be turned off when it is
set below 25°C (The display will show OFF –T, °C –set point)
Program execution
After the PCMT has reached a stable temperature (when the set and current temperature
readings become the same):
4.6 Insert tubes into the block or place the microwell plate on the block and close the lid.
4.7 Press the RPM-RUN/STOP key Fig. 3/4 the block will start rotation and the timer indicator will
start counting up the time interval (with 1 min precision).
Note! If the rotation speed is set to zero, pressing RPM-RUN/STOP key starts the timer but the
block does not move.
Attention! Automatic balancing system in this product produces light metal-like noise when moving
the unit which is likely to be heard during acceleration/deceleration. It is normal
occurrence and does not indicate a fault.
4.8 At the end of the program (after the set time elapses) the block motion stops and the timer
shows the flashing reading STOP accompanied by the repetitive sound signal until the RPM-
RUN/STOP key is pressed.
4.9 If the working time is not set (or is re-set) and the timer indicator in the upper line shows
00:00, pressing the RPM-RUN/STOP button will start continuous operation of the device with
the countdown timer in the lower line (Actual), until the RPM-RUN/STOP button is pressed
again.
4.10 If required, it is possible to restart the timer when it is running. Press the TIME-RUN/STOP key
once Fig. 3/5 to stop the timer. Press the TIME-RUN/STOP key again to restart the timer.
4.11 The block motion can be stopped at any time by pressing the RPM-RUN/STOP key. In this
case the program and the thermo block motion stops and timer is set back to zero switching
into the STOP mode. Press the RPM-RUN/STOP key to repeat the operation with the same
time and speed.
4.12 At the end of operation turn OFF the unit with the power switch at the rear panel and
disconnect the external power supply from the mains.

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5. Calibration
5.1. The operating temperature of the device is pre-calibrated at the factory (the calibration
coefficient is 1.00) which is measured by an external sensor installed in the heating block.
5.2. To enter the calibration coefficient, hold the TIME-RUN/STOP key (Fig. 3/5) for more than 8
sec to activate calibration mode. The calibration coefficient will be shown on the display (Fig.
5/1).
5.3. Set 1.000 value using the ▲ and ▼ keys (Fig. 3/3) as shown on Fig. 5/1 to restore the factory
settings.
5.4. PresstheRPM-RUN/STOPkeyoncetoexitthecalibrationmode.
Calibration procedure
5.5. Install an independent temperature sensor (thermometer 0.5 C accuracy) into the tubes, placed
into the block,ordirectlyintotheheatingblockspecialsensorsocket.
5.6. Set therequiredtemperatureinoperationmode(e.g.40°C).
5.7. Aftertheunitreachesthesettemperature(whenthesetand actual temperature readingsareequal)
leavetheunitfor30minforthermalstabilization.
5.8. Let us assume that the reading of the independent temperature sensor is 39°C, but the display’s
actual temperature is 40 °C (Fig.4). Then it is necessary to add 1°C correction.
Fig.4 Control panel in operation mode
5.9. Hold down the TIME-RUN/STOP key (Fig. 3/5) for more than 8 sec. to activate calibration
mode. Thefollowingparameterswillbeshownonthedisplay(Fig.5)
Fig.5 Control panel in calibration mode

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Fig.6 Control panel in calibration and operation mode
5.10. Set the temperature with multiplier coefficient readings (Fig 6A/1) to change the actual
temperature value of the device.
Using the Temp. ▲and ▼ keys (Fig.3/3), change the calibration coefficient (Fig.6A/1) so, that
the new temperature value (Fig.6A/2) corresponds to the independent temperature sensor. In
ourexamplethecalibrationcoefficientwillbe 0.974(inrange:0.936upto1.063;increment0.001).
Caution! This calibrating coefficient will correct the temperature through the whole operating
range.
5.11. After finishing the calibration press the RPM-RUN/STOP key (Fig.3/4) once to save the
changes and exit the calibration mode.
5.12. The display will show calibrated temperature as shown on fig.6B/3 and the unit will continue
thermal stabilization according to the previously set temperature.

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6. Specifications
6.1 Operating conditions
The unit is designed for operation in cold rooms, incubators and laboratory rooms at
ambient temperature from +4ºC to +40ºCand maximum relative humidity80%for temperatures up
to31°Cdecreasinglinearlyto50%relativehumidityat40°C.
6.2 Temperature specifications
Temperature setting range ....................................................................................................+4 to +100°C
Temperature control range ....................................................................................15 below RT to +100°C
Temperature setting resolution .........................................................................................................0.1°C
Temperature stability.........................................................................................................................±0.1°C
Temperature accuracy at +37ºC ......................................................................................................±0.5°C
Temperature uniformity over the block at + 37°C..............................................................................±0.1ºC
Average heating speed on HC20C from +25°C to +100°C...........................................................5ºC/min
Average cooling speed on HC20C from +100°C to RT (+25°C)...................................................5ºC/min
from RT (+25°C) to 15°C below RT ....................................1.8ºC/min
Time of block heating from +25ºC till +37ºC ....................................................................................6 min
Temperature calibration coefficient range………………………….………….from 0.936 to 1.063 (±0.063)
6.3 General specifications
Speed range ......................................................................................................................250 -1400 rpm
Speed setting resolution ................................................................................................................10 rpm
Maximal speed deviation
for 250 rpm....................................................................................................................................2%
for 1400rpm...............................................................................................................................0.7%
Digital time setting................................................................................................................1 min - 96 hrs
Time setting resolution ......................................................................................................................1 min
Maximum continuous operation time ........................................................................................... 96 hours
(Recommended interval between operation sessions not less than 8 hours)
Orbit ..................................................................................................................................................2 mm
Display .............................................................................................................................16x2 signs, LCD
Dimensions ....................................................................................................................205x230x130 mm
Input current/power consumption .....................................................................................12V, 4.9 A/ 60W
External power supplyunit................................................... input AC 100-240 V 50/60Hz, output DC 12V
Weight with block and external power supply, not more ..................................................................3.7 kg

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Optional accessories
Description
HC15
block for 20x1.5 ml microtubes
HC32
block for 20x0.2 ml microtubes + 12x1.5 ml microtubes
HC20
block for 20x2.0 ml microtubes
HC18
block for 20x0.5 ml + 12x1.5 ml microtubes
HC24
block for 24x2 ml microtubes
HC96
block for 96-well PCR microplate (semi-skirted, unskirted)
PCMT blocks and PHMT blocks are not interchangeable, i.e. PCMT blocks cannot be used on the
PHMT andviceversa.
Replacement parts
Description
Rubber belt
117x5x0.6 mm
Grant is committed to a continuous program of improvement, and reserves the right to alter the design
and specification without notice.

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7. Guarantee and Service
6.1 Guarantee
When used in laboratory conditions and according to these working Instructions, this
product is guaranteed for TWO YEARS against faulty materials or workmanship.
6.2 Service
For service, return for repair to our Service Department in the UK or, in other countries,
to our distributor.
6.3 Rubber belt replacement
1. Disconnect the external power supply from the mains.
2. Remove 4 fixation screws on the shaker bottom and remove the bottom plate.
3. Replace the rubber belt.
4. Re-assemble the unit.

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Grant Instruments
(Cambridge) Ltd
Shepreth
Cambridgeshire
SG8 6GB
UK
Tel: [phone]
Fax: [phone]
Email:[email protected]
www.grantinstruments.com