Greiner 852076-na User manual

INSTRUCTIONS

FOR USE

CRYO.S 6-CHANNEL

HANDHELD DECAPPER

Item No.: 852076 -NA/ -UK/

-EU/ -AU/ -JP/ -CN

2 / 26 INSTRUCTION FOR USE

IMPORTANT NOTICE:

This manual may not be used or reproduced in any way whatsoever without the express written consent of Greiner Bio-One

GmbH. The Greiner Bio-One GmbH address can be found on the last page. Greiner Bio-One GmbH is responsible for this

operator’s manual.

3 / 26CRYO.S 6-CHANNEL HANDHELD DECAPPER

INSTRUCTION MANUAL

INTRODUCTION:

This manual helps users operate the Cryo.s 6-Channel Handheld Decapper correctly

and safely, herein referred to as the Decapper. To achieve this goal, the manual

describes the different components of the Decapper and their functionality.

After introducing you to the various parts of the Decapper, the manual will describe

step by step how to operate the Decapper. After you have read through this manual,

you should be capable of operating the Decapper.

In order to prevent injury to personnel and damage to equipment the manual contains

the following precautionary statements:

WARNING

The WARNING statement describes a potentially hazardous situation

which if not avoided could result in serious injury of personnel and dam-

age to equipment.

CAUTION

The CAUTION statement describes a potentially hazardous situation

which if not avoided could result in damage to equipment and minor

injury.

NOTICE

The NOTICE statement indicates information considered important but

not hazard related.

FURTHER INFORMATION:

Animated Video of the Handheld Decapper

available here.

The animated demonstration provides a brief overview of how to

utilize the decapper in just a few seconds, but it does not serve as

a substitute for the comprehensive Instruction for Use.

4 / 26 INSTRUCTION FOR USE

/1. Information about Intended Use, Safety and Security ..................................... 6

1.1. Disclaimer Statement ............................................................................................... 6

1.2. Intended Use ............................................................................................................. 6

1.3. Legal Regulations ..................................................................................................... 7

1.4. Malfunction / Troubleshooting .................................................................................. 8

1.5. Precautions and Safety ............................................................................................. 8

1.5.1. Maintenance, Service and Repair........................................................................ 8

1.5.2. Operating the Instrument ................................................................................... 8

1.5.3. Electrical Safety Precautions ............................................................................ 9

1.5.4. Hazards .............................................................................................................. 9

1.5.5. Safety Measures ............................................................................................... 10

1.5.6. Reporting Serious Incidents ............................................................................. 10

/2. Decapper Introduction .................................................................................. 11

2.1. Decapper Design Overview ...................................................................................... 11

2.2. Concept Overview ................................................................................................... 12

/3. Installation and Set-up .................................................................................. 13

3.1. Shipment ................................................................................................................ 13

3.2. Hardware Installation .............................................................................................. 13

3.2.1. Power Cable Connection................................................................................... 13

3.2.2. Docking the Decapper ...................................................................................... 14

3.2.3. Disposal of Shipment Materials ........................................................................ 14

/4. Working with the Decapper .......................................................................... 14

4.1. Decapping Tubes ..................................................................................................... 14

4.2. Storing Caps ........................................................................................................... 15

4.3. Capping Tubes ......................................................................................................... 15

4.4. Ejecting Caps........................................................................................................... 15

4.5. Wireless Operation .................................................................................................. 16

4.6. Charging .................................................................................................................. 17

4.7. Adjusting Capping Torque........................................................................................ 18

/5. Ordering Information .................................................................................... 20

/6. Troubleshooting ........................................................................................... 21

TABLE OF CONTENT:

5 / 26CRYO.S 6-CHANNEL HANDHELD DECAPPER

/7. Cleaning Maintenance .................................................................................. 22

7.1. Materials Required................................................................................................... 22

7.1.1. Cleaning Agents ................................................................................................ 22

7.2. Cleaning Parts of the Instrument ............................................................................. 23

/8. TechnicalSpeciations ................................................................................ 23

/9. Declaration of Conformity ............................................................................ 24

/10. Compliance with the European Union’s Waste Electrical

& Electronic Equipment Directive ................................................................. 25

/11. RoHS Compliance ......................................................................................... 25

/12. Standard Warranty ....................................................................................... 25

6 / 26 INSTRUCTION FOR USE

1.1/ DISCLAIMER STATEMENT

This user manual is designed to provide guidance and information for users who are

working with the Decapper. It covers the most encountered procedures, handling

techniques, and safety considerations for daily use. Please note that the content of

this manual is subject to change without notice, as Greiner Bio-One GmbH continues

to develop new products, accessories, and software. It is important to recognize

that this manual may not be comprehensive in its explanations, instructions, or

statements,andmaynotreectthemostcurrentinformationavailable.Someillu-

strations, images, and descriptions may also be incomplete or vary from the actual

product received. Our goal is to provide the most accurate and up-to-date information

possible, but we cannot guarantee its completeness or accuracy.

1.2/ INTENDED USE

The Decapper provides a fast and robust method to cap and uncap Cryo.s non-coded

Tubes and Cryo.s with Barcode and Datamatrix Code in SBS 48-format racks. A list of

compatible products from Greiner Bio-One GmbH is provided hereinafter.

The Decapper is a handheld standalone instrument designed to quickly and reliably

decap and cap six tubes in parallel. The device will be used in conjunction with a

docking station for storage and charging when not in use.

NOTICE

You should carefully read through the entire manual before beginning to

operateyourDecapper.Thisrstsectionmustbereadwithattention.

It contains important information about the use of the Decapper and

this manual.

1/ INFORMATION ABOUT INTENDED USE,

SAFETY AND SECURITY

This User’s Manual is designed to help you get the most out of your Decapper.

7 / 26CRYO.S 6-CHANNEL HANDHELD DECAPPER

Item No. Description

123263 Cryo.s non-coded tubes, 1 ml, starfoot base, internal tube thread

122263 Cryo.s non-coded tubes, 2 ml, starfoot base, internal tube thread

126263 Cryo.s non-coded tubes, 2 ml, starfoot base, external tube thread

127263 Cryo.s non-coded tubes, 4 ml, starfoot base, external tube thread

123263-2DG Cryo.s non-coded tubes, 1 ml, starfoot base, internal tube thread,

linearbarcode,humanreadableidentieranddatamatrixcode

122263-2DG Cryo.s non-coded tubes, 2 ml, starfoot base, internal tube thread,

linearbarcode,humanreadableidentieranddatamatrixcode

126263-2DG Cryo.s non-coded tubes, 2 ml, starfoot base, external tube thread,

linearbarcode,humanreadableidentieranddatamatrixcode

127263-2DG Cryo.s non-coded tubes, 4 ml, starfoot base, external tube thread,

linearbarcode,humanreadableidentieranddatamatrixcode

803202 48-formatRackforCryo.s,lowprolefor1and2mlCryo.s

803270 48-formatRackforCryo.s,highprolefor4mlCryo.s

803202-2DG 48-formatRackforCryo.s,lowprolefor1and2mlCryo.s,

linearbarcode,humanreadableidentieranddatamatrixcode

803270-2DG 48-formatRackforCryo.s,highprolefor4mlCryo.s,

linearbarcode,humanreadableidentieranddatamatrixcode

803507 48-format Cap Carrier

In addition, the Decapper may be used with Cryo.s non-coded Tubes and Cryo.s with

Barcode and Datamatrix Code with coloured screw cap versions as well as customized

barcodes (REF 12x2xx, 12x2xx-2dx).

1.3/ LEGAL REGULATIONS

All local, state and federal laws which prescribe the use, application, and/or the

handling of dangerous materials in connection with the Decapper must be strictly

followed.

8 / 26 INSTRUCTION FOR USE

1.4/ MALFUNCTION / TROUBLESHOOTING

Refer to the section: 6 Troubleshooting/page 21.

1.5/ SAFETY PRECAUTIONS

NOTICE

For a malfunction and for troubleshooting the Decapper, contact your

local Greiner Bio-One representative.

NOTICE

Read the following safety notices carefully before using the Decapper

1.5.1/ MAINTENANCE, SERVICE AND REPAIR

Only trained contractual partners of Greiner Bio-One are authorized to perform

mechanical repair on the Decapper.

1.5.2/ OPERATING THE INSTRUMENT

When using the Decapper, Good Laboratory Practices (GLP) must be observed.

Suitable protective clothing, safety glasses and protective gloves must be worn.

WARNING

During Decapper operation, keep clear of all moving parts. Never

interfere with the Decapper while it is in operation!

CAUTION

IftheDecapperisusedinamannernotspeciedbyGreinerBio-One,

the protection provided by the device may be impaired.

9 / 26CRYO.S 6-CHANNEL HANDHELD DECAPPER

1.5.3/ ELECTRICAL SAFETY PRECAUTIONS

There are no electrical components that are accessible to the user in the Decapper.

WARNING

Any removal of the housing to access internal components must only be

performed by contractual partners of Greiner Bio-One.

1.5.4/ HAZARDS

The following section describes the main safety considerations, electrical and bio-

logical, in operating this product and the main hazards involved.

CAUTION

Only authorized personnel may have access to the device. The laboratory

or operator of the instrument is responsible for ensuring that the envi-

ronmental conditions for safe operation of the Decapper are always met.

The laboratoryoroperatorisresponsible forany improperhandling. Itmust

be ensured that untrained or unauthorized personnel cannot manipulate

or interfere with the Decapper while it is in use.

10 / 26 INSTRUCTION FOR USE

CAUTION

Use appropriate protective clothing,

goggles and gloves!

1.5.5/ SAFETY MEASURES

1.5.6/ REPORTING SERIOUS INCIDENTS

Any serious incident or near incident that has occurred in relation to the Decapper shall

be reported to Greiner Bio-One and to the competent authority of the member state

in which the operator is established. Contacting Greiner Bio-One either by contac-

ting a local Greiner Bio-One representative or by utilizing the Greiner Bio-One webpage,

which can be found on the last page of this operating manual.

11 / 26CRYO.S 6-CHANNEL HANDHELD DECAPPER

2/ DECAPPER INTRODUCTION

2.1/ DECAPPER DESIGN OVERVIEW

The following picture depicts the main components of the Decapper.

Handheld Decapper Device

Battery Indicator

Torque Indicator Panel

Torque Button

Adaptors

Docking Station

Cap Button

Eject Button

Decap Button

Battery Indicator

12 / 26 INSTRUCTION FOR USE

2.2/ CONCEPT OVERVIEW

The Decapper provides a precise method for processing six tubes in parallel. An inte-

grated torque control panel allows to pre-set torque levels. For details on torque set-

ting please refer to 4.7 Adjusting Capping Torque/ page 18.

The docking station and integrated re-chargeable battery provide the convenience

ofwirelessoperationwitheaseofuseforchargingintegrationintoyourworkow.

The Decapper is an intuitive and ergonomic device, designed to simplify your capping

and decapping processes.

The Decapper comprises the following functions:

/Adjustable torque control

/Wireless operation

/Magnetic docking station

/Supports SBS-format tube racks

To guarantee proper tube capping and decapping with the Decapper, it is necessary

tofullthefollowingfunctions:

1. Tubes must be presented in complementary SBS 48-format racks.

2. The cap button must be depressed to actuate the motors to rotate counter-

clockwise.

3. The decap button must be depressed to actuate the motors to rotate clockwise.

4. The torque button must be depressed to change the applied capping torque.

5. The eject button must move the ejector block vertically to detach

adaptors from caps

NOTICE

For Greiner Bio-One's cryogenic tubes with internal and external threads,

torque pre-setting levels 4 and 5 (4/5 green LEDs lit up) were found to be

optimal, respectively.

13 / 26CRYO.S 6-CHANNEL HANDHELD DECAPPER

3/ INSTALLATION AND SET-UP

These instructions allow operators to independently install the Decapper device into

their workspace.

3.1/ SHIPMENT

Your delivery consists of the following items:

/Decapper device

/Docking station

/Power cord & power adaptor

/Adaptors (pre-installed)

/Adaptors case

/Manual

/Quick start guide

3.2/ HARDWARE INSTALLATION

3.2.1/ POWER CABLE CONNECTION

Connect the power cable as shown in the area on the docking station indicated by

the arrow

14 / 26 INSTRUCTION FOR USE

3.2.2/ DOCKING THE DECAPPER

Before initial use the Decapper should be fully charged. After removal from pack-

aging, the Decapper should be placed in the Docking Station until a full charge is

indicated.

3.2.3/ DISPOSAL OF SHIPMENT MATERIALS

It is the customer’s responsibility for the care and disposal of all packaging materials.

4/ WORKING WITH THE DECAPPER

4.1/ DECAPPING TUBES

Fullyinserttheadaptorsintotheinnercavitiesofthesixcorrespondingcapsbyrmly

pressingtheDecapperdown.Theejectionblockshouldbeushwiththetopsofthe

tubes. Press and hold the decap button to start the decapping operation. During de-

capping, press the device down. While decapping, slowly move the device upwards.

DecappingCorrect Incorrect

15 / 26CRYO.S 6-CHANNEL HANDHELD DECAPPER

4.2/ STORING CAPS

Capscanstayontheadaptorsduringpipettingorotheraspectsofyourworkow.

The device can be placed in the docking station while not in use. Alternatively, the

caps can be ejected into a cap carrier. To dock the device, simply place the Decapper

intothedockingstationandslideitrmlytotheback.Wheninstalledproperlythe

status LED should display a charging or battery full status.

4.3/ CAPPING TUBES

Position the device with the attached screw caps directly above the tube openings.

Gently press the device down while holding the cap button to begin the capping pro-

cess. Continue pressing downwards on the tube while holding the cap button until

the rotation of all adaptors come to a complete stop.

4.4/ EJECTING CAPS

When done capping a row of tubes, release pressure on the device and press the

eject button until you feel the device lift off the plate. Press the eject button com-

pletely down to ensure the caps are fully unseated from the adaptors.

NOTICE

Ensuretheejectionblockisparallelandushtothecapssothatcaps

are retained properly.

NOTICE

The Greiner Bio-One Cap Carrier in 48-format REF 803507 can be used

as a cap holder for Cryo.s non-coded Tubes and Cryo.s with Barcode and

Datamatrix Code. These Cap Carriers are not included with the tubes

and must be purchased separately.

NOTICE

After capping, carefully check that screw caps are applied correctly,

with no protruding caps or squeezed-out sealing gaskets. This semi-au-

tomatic device requires manual visual inspection to identify and correct

rare cases of improper sealing.

16 / 26 INSTRUCTION FOR USE

4.5/ WIRELESS OPERATION

The Decapper only operates wireless. The battery indicator will display the battery

status when in use. This will remain on for ~15 seconds after either the cap or decap

buttons have been pressed. The below table shows the power status levels displayed

by the Decapper. The power indicator will also ash every ~8 seconds while the

device is not in use.

Colour Battery Status Appearance

Solid green Good battery level.

Solid yellow Battery level low.

Charge when possible.

Solid red Critical battery level.

Device is disabled.

Charge immediately.

17 / 26CRYO.S 6-CHANNEL HANDHELD DECAPPER

Colour Battery Status Appearance

Solid blue Fully charged.

Flashing green Actively charging.

Flashing red Charging error.

4.6/ CHARGING

When not in use, place the device in the docking station to automatically charge. The

power indicator will indicate the charging state of the device when properly installed.

The motors are disabled, and the device cannot be used while in the docking station.

The below table shows the charging status indicators.

18 / 26 INSTRUCTION FOR USE

4.7/ ADJUSTING CAPPING TORQUE

The Decapper comes programmed with 5 pre-set torque levels. Press the torque but-

ton once to light up the torque indicator panel. The number of LEDs lit up indicates

the set torque level. To change the level, press the torque button within 2 seconds to

increment the torque level by one. Repeat the previous step until the desired torque

level is reached. After reaching level 5, the torque will cycle to level 1. The below

table shows the torque level and the approximate capping torque applied at that level.

Torque Level Indicator Capping Torque Display

170 mNm ± 10 mNm

290 mNm ± 10 mNm

3120 mNm ± 10 mNm

NOTICE

Torque pre-setting level 4 (four green LEDs of the torque indicator lit up)

was found optimum for capping Greiner Bio-One’s Cryo.s non-coded Tubes

and Cryo.s with Barcode and Datamatrix Code with internal thread.

Torque pre-setting level 5 (vegreenLEDsofthetorqueindicatorlitup)

was found optimum for capping Greiner Bio-One’s Cryo.s non-coded Tubes

and Cryo.s with Barcode and Datamatrix Code with external thread.

19 / 26CRYO.S 6-CHANNEL HANDHELD DECAPPER

Torque Level Indicator Capping Torque Display

4200 mNm ± 10 mNm

5250 mNm ± 10 mNm

NOTICE

Do not apply too high torque levels as this may destroy the thread on the

tube and tubes may not be closed properly any longer. Leakage may occur.