Hach LBOD101 User manual

DOC022.53.80025
LBOD101
05/2021, Edition 4
User Manual

Table of Contents
Section 1 Product overview................................................................................. 3
Section 2 Specifications........................................................................................ 3
Section 3 Safety information............................................................................... 4
3.1 Intended use ....................................................................................................... 4
3.2 Use of hazard information..................................................................................... 4
3.3 Precautionary labels............................................................................................. 5
3.4 Product hazards.................................................................................................... 5
Section 4 Preparation for use.............................................................................. 5
Section 5 Calibration.............................................................................................. 6
5.1 Calibration notes................................................................................................... 6
5.2 LBOD water-saturated air (100%) calibration procedure...................................... 6
5.3 Zero-point calibration procedure........................................................................... 7
Section 6 Sample measurement ...................................................................... 8
6.1 Sample measurement notes................................................................................. 8
6.2 Sample measurement procedure......................................................................... 8
6.3 Low-level measurements...................................................................................... 9
Section 7 Maintenance........................................................................................... 9
7.1 Clean the probe.................................................................................................... 9
7.2 Replace the sensor cap and iButton..................................................................... 9
7.3 Replace the stirrer.............................................................................................. 12
7.4 Storage............................................................................................................... 12
Section 8 Troubleshooting................................................................................. 13
Section 9 Consumables....................................................................................... 14
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Table of Contents
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Section 1 Product overview
The Intellical LBOD101 probe is a digital, luminescent dissolved oxygen sensor that measures the
dissolved oxygen concentration of BOD (biochemical oxygen demand) samples. The probe has
temperature and absolute air pressure sensors for accurate dissolved oxygen measurements. The
probe stays on standard BOD bottles and stirs the sample during measurements. Refer to Figure 1.
Figure 1 Probe overview
1 LBOD sensor cap 6 Stirrer on/off button
2 Thermistor 7 Power indicator LED
3 Stirrer assembly 8 Air pressure sensor module
4 Probe lens 9 iButton® compartment1
5 Probe body with stirrer assembly
Section 2 Specifications
Specifications are subject to change without notice.
Specifications Details
Probe type Luminescent dissolved oxygen (LDO) sensor with integrated stirring
system
Dissolved oxygen range 0.05 to 20.0 mg/L (ppm); 1 to 200% saturation
Dissolved oxygen accuracy ±0.05 mg/L for concentrations less than 10 mg/L O2
±0.1 mg/L for concentrations more than 10 mg/L O2
1iButton is a registered trademark of Maxim Integrated Products, Inc.
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Specifications Details
% saturation resolution 0.1%
% saturation accuracy ±0.59% of reading
Stabilization time T90% at 10 seconds (when the solution is stirred)
Temperature resolution 0.1 °C (0.18 °F)
Temperature accuracy ±0.3 °C (±0.54 °F)
Pressure resolution 5 mbar (0.5 kPa)
Pressure accuracy ±3 mbar (0.3 kPa)
Operating temperature 0 to 50 °C (32 to 122 °F)
Storage temperature 5 to 40 °C (41 to 104 °F)
Minimum immersion depth 10 mm (0.394 in.)
Dimensions Diameter: 15.875 mm (0.625 in.)
Length: 215 mm (8.46 in.)
Cable length: 1.8 m (5.9 ft)
Cable connection M12 digital output and connector
Warranty 1 year on the probe. This warranty covers manufacturing defects, but
not improper use or wear.
Certifications CE, FCC/ISED
Section 3 Safety information
3.1 Intended use
The Intellical probes are intended for use by individuals who measure water quality parameters in the
laboratory or in the field. The Intellical probes do not treat or alter water.
3.2 Use of hazard information
D A N G E R
Indicates a potentially or imminently hazardous situation which, if not avoided, will result in death or
serious injury.
WARNING
Indicates a potentially or imminently hazardous situation which, if not avoided, could result in death
or serious injury.
CAUTION
Indicates a potentially hazardous situation that may result in minor or moderate injury.
N O T I C E
Indicates a situation which, if not avoided, may cause damage to the instrument. Information that
requires special emphasis.
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3.3 Precautionary labels
Read all labels and tags attached to the instrument. Personal injury or damage to the instrument
could occur if not observed. A symbol on the instrument is referenced in the manual with a
precautionary statement.
Electrical equipment marked with this symbol may not be disposed of in European
domestic or public disposal systems. Return old or end-of-life equipment to the
manufacturer for disposal at no charge to the user.
3.4 Product hazards
CAUTION
Chemical exposure hazard. Obey laboratory safety procedures and wear all of the
personal protective equipment appropriate to the chemicals that are handled. Refer to
the current safety data sheets (MSDS/SDS) for safety protocols.
CAUTION
Chemical exposure hazard. Dispose of chemicals and wastes in accordance with local,
regional and national regulations.
Section 4 Preparation for use
Prepare the probe for calibration and measurement as follows. Do not touch the protective black
layer on the LBOD sensor cap.
1. Open the pressure sensor module cap and make sure that the label on the iButton is on top.
2. Rinse the probe with deionized water. Blot dry with a lint-free cloth.
3. Make sure that the meter has the correct date and time settings. The service-life time stamp in
the probe comes from the date and time settings in the meter.
Note: Some meters automatically open the date and time settings when the meter starts for the first time, or
after battery replacement.
4. Connect the probe to the meter.
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Section 5 Calibration
The procedure that follows is applicable to meters that can connect to Intellical LBOD probes. Refer
to the applicable meter documentation for meter operation and probe-specific settings.
5.1 Calibration notes
Read the notes that follow before calibration.
• Measure samples as soon as possible after collection.
• Use the single display mode for calibration when more than one probe is connected to the meter (if
applicable).
• Calibrate the probes and verify the calibration regularly for best results. Use the meter to set
calibration reminders.
• The calibration data is stored in the probe. When a calibrated probe is connected to a different
meter with the same calibration options, a new calibration is not necessary.
• Air bubbles below the sensor when in solution can cause a slow response or error in the
calibration. Make sure to remove air bubbles during calibration.
• The meter uses the slope value shown at the end of the calibration to monitor the condition of the
sensor cap.
5.2 LBOD water-saturated air (100%) calibration procedure
1. Fill a BOD bottle
approximately ¾ full
with water.
2. Put a stopper on
the bottle and shake
for 30 seconds.
3. Rinse the probe
with deionized water.
Dry the probe with a
lint-free cloth.
4. Carefully dry the
LBOD sensor cap
with a non-abrasive
cloth.
5. Put the probe in
the bottle. Wait
approximately
10 minutes for the
contents to adjust to
ambient
temperature.
6. Go to the
calibrate menu.
Select the probe, if
applicable.
7. Read the
dissolved oxygen
value. The display
shows 100% when
the reading is stable.
8. Save the
calibration.
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5.3 Zero-point calibration procedure
A zero-point calibration can increase the measurement accuracy of samples that have less than
1 mg/L O2. A zero point calibration is not recommended for samples that have more than 1 mg/L O2.
Set the calibration mode to "100% with 0". The meter starts the calibration with the 100%
measurement, then continues to the zero-point measurement.
1. Fill a BOD bottle
with deionized water.
2. Add 300 mg of
sodium sulfite to the
bottle.
3. Add 2 mL of
cobalt chloride
solution to the bottle.
4. Put a stopper on
the bottle and fully
mix for 30 seconds.
5. Remove the
stopper. Put the
probe in the bottle.
6. Tap the probe to
remove air bubbles.
7. Wait
approximately
10 minutes for the
contents to adjust to
ambient
temperature.
8. Start the stirrer.
9. Read the
dissolved oxygen
value. Wait for the
measurement to
become stable.
10. Save the
calibration.
11. Rinse the probe
with deionized water.
Dry the probe with a
lint-free cloth.
12. Fill a BOD bottle
with deionized water.
Put the probe in the
bottle and stir for
10 minutes to fully
remove the sulfite
from the probe.
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Section 6 Sample measurement
The procedure that follows is applicable to meters that can connect to Intellical LBOD probes. Refer
to the applicable meter documentation for meter operation and probe-specific settings.
6.1 Sample measurement notes
Read the notes that follow before sample measurements.
• Salinity changes the solubility of oxygen in water. Measure the sample salinity and enter the value
in the probe settings of the meter.
• High concentrations (more than 1 molar) of acids or bases will decrease the service life of the
LBOD sensor cap.
• Rinse the probe with deionized water and dry with a lint-free cloth between measurements to
prevent contamination.
• If complete traceability is necessary, enter a sample ID and operator ID before measurement.
Refer to the meter manual for instructions.
• The meter automatically saves the measurement data when the user manually reads each data
point and when the meter is set to read at regular intervals. The user must manually save each
data point when the meter is set to read continuously.
• Air bubbles below the sensor can cause a slow response or error in the measurement. Make sure
to remove air bubbles before and during measurements.
6.2 Sample measurement procedure
1. Rinse the probe
with deionized water.
Dry the probe with a
lint-free cloth.
2. Put the probe in
the BOD sample.
3. Tap the probe to
remove air bubbles.
4. Start the stirrer.
5. Read the
dissolved oxygen
value of the sample.
The display shows
the dissolved oxygen
value when the
reading is stable.
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6.3 Low-level measurements
If samples are expected to have less than 1 mg/L O2, calibrate the probe with a zero-oxygen solution.
A zero-point calibration is not recommended for samples that have more than 1 mg/L O2. Use the
guidelines and steps that follow only when the sample is expected to have less than 1 mg/L O2.
• Calibrate the probe with a zero-oxygen solution. Use the "100% with 0" calibration option in the
meter. Measure the 100% value first, then measure the zero-oxygen solution. Refer to Zero-point
calibration procedure on page 7.
• Measure the salinity of the sample and enter the salinity correction factor in the probe settings.
• Wait approximately 10 minutes for the measurement contents to adjust to ambient temperature,
then measure the sample. Measure the sample again to make sure the result is the same.
• Clean the probe regularly. Refer to Clean the probe on page 9.
Section 7 Maintenance
7.1 Clean the probe
N O T I C E
The LBOD sensor cap has a protective black layer to increase the life of the LBOD sensor. Do not
rub the black layer to clean the LBOD sensor cap. Do not use alcohol or other organic solvents to
clean the LBOD sensor cap.
Keep the LBOD sensor cap clean for best results. Use only water and neutral detergents to clean the
probe.
1. Put the probe in a neutral cleaning solution and stir the solution. Do not rub or remove the black
layer on the LBOD sensor cap.
2. Rinse the probe with deionized water. Blot dry with a lint-free cloth.
7.2 Replace the sensor cap and iButton
N O T I C E
Do not use sharp metal tools to remove the LBOD sensor cap.
Replace the LBOD sensor cap after 365 days, or more frequently if the cap becomes damaged or
dirty. The LBOD sensor cap and iButton operate together and must be replaced at the same time.
1. Use fingers to remove the LBOD sensor cap from the probe. Do not touch the probe lens. Refer
to Figure 2. Discard the used LBOD sensor cap.
Note: The stirrer assembly can stay installed.
2. If water is between the LBOD sensor cap and the probe lens, or if the probe lens looks dirty, rinse
with dilute isopropyl alcohol (10% or less) or deionized water and blot dry with a non-abrasive
cloth. Do not wipe the probe lens or use abrasive cleaners.
3. Hold the new LBOD sensor cap by the sides and install the new cap on the probe. Refer to
Figure 2. Make sure the LBOD sensor cap is on tightly.
Note: Do not touch the protective black layer on the LBOD sensor cap.
4. Install the new iButton® in the pressure sensor module. Refer to Figure 3. Make sure that the
label on the iButton is on top when the cap is open. Make sure to fully close the cap.
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Figure 2 Replace the LBOD sensor cap
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Figure 3 Replace the iButton
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7.3 Replace the stirrer
Refer to Figure 4 to replace the stirrer assembly.
Figure 4 Replace the stirrer
7.4 Storage
Keep the probe in a BOD bottle with some water (fill to ¼ minimum) when not in use.
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Section 8 Troubleshooting
Problem Possible cause Solution
Decreased probe
performance causes slow
stabilization and prevents
accurate calibrations or
measurements.
The sensor cap is loose or
damaged.
Tighten or replace the sensor cap.
Always replace the sensor cap and the
iButton at the same time. Refer to
Replace the sensor cap and iButton
on page 9.
Water is between the
sensor cap and the probe
lens.
Remove the sensor cap and dry the
probe lens. Refer to Replace the
sensor cap and iButton on page 9.
The sensor cap is not
sufficiently conditioned.
Keep the probe in the BOD bottle with
some water for more time, then try the
calibration again.
The temperature or
pressure sensor does not
operate correctly.
Compare the temperature and
pressure readings of the probe with
external measurements. The pressure
sensor reads absolute pressure, which
is not adjusted to sea level. If the
measurements are not correct, contact
technical support.
The lot code on the iButton
is not the same as the lot
code on the sensor cap.
Replace the sensor cap and iButton.
The stirrer does not operate
correctly.
The stirrer does not
operate.
The stirrer uses the power from the
meter to operate. Make sure to start
the meter, then start the stirrer.
The stirrer turns only when
the probe is not in a BOD
bottle.
Remove and install the stirrer
assembly. Refer to Replace the stirrer
on page 12.
The stirrer makes a lot of
noise.
Remove and install the stirrer
assembly. Refer to Replace the stirrer
on page 12.
Sample properties cause
slow stabilization or
inaccurate measurements.
The measurement was not
adjusted for salinity in the
sample.
Measure the salinity of the sample and
enter the value as a salinity correction
factor in the meter.
Procedure problem causes
slow stabilization and
prevents accurate
calibrations or
measurements.
Air bubbles are around or
below the probe tip.
Carefully tap or shake the probe to
remove air bubbles.
The contents of the BOD
bottle did not fully adjust to
ambient temperature.
Wait more time for the contents of the
BOD bottle to adjust to ambient
temperature.
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Section 9 Consumables
Note: Product and Article numbers may vary for some selling regions. Contact the appropriate distributor or refer to
the company website for contact information.
Description Quantity Item no.
LBOD sensor cap replacement kit (includes iButton) 1 5838000
LBOD probe replacement stirrer assembly 1 5850800
LBOD stirrer bearing clip 1 5852800
BOD bottle with glass stopper, 300 mL 1 62100
Beaker, 250 mL, polypropylene 1 108046
Cobalt chloride solution 500 mL 1422249
Sodium sulfite 100 g 2386026
Disposable wipes, 11 x 22 cm 280/pkg 2097000
Wash bottle, polyethylene, 500 mL 1 62011
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*DOC022.53.80025*
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