Haier HCP-80 User manual

Operation Instruction
of CO2 Incubator
Model:
HCP-80
HCP-168
HCP-258
• Please read this manual carefully before using the equipment.
• The company reserves the right to interpret the content of this manual.
• Please keep it with your you for future reference.
• Product technology or software is subject to upgrade without prior notice.
• The image in this manual does not necessarily refl ect the product appearance of the actual product.

Content
Safety ......................................................................... 1
Precautions for Use ................................................... 4
Product Description .................................................... 5
Product lnstallation ..................................................... 7
Description of Parts • Control Panel ...........................11
Commissioning and application ................................. 13
Alarm ......................................................................... 19
Cleaning and sterilization .......................................... 20
CO2 concentration calibration .................................... 26
Periodic maintenance ................................................ 28
Q&A ........................................................................... 29
Circuit diagram .......................................................... 30
Specifi cation • Packing List ........................................ 31

1
Dear Haier Biomedical Customers:
Thank you for choosing Haier CO2 incubator. To prevent potential personnel injury and product
damage, we recommend that the contents of the manual be followed closely. Pay special attention to
the following symbols in this manual. The CO2 incubator will be referred to as incubator hereinafter.
Earthing
Mark
Warning High
Temperature
Caution, risk of
electric shock
It is very important to read this instruction manual when using this machine. Safety protections
provided by the device may be damaged if they are not followed in accordance with the methods
specifi ed in this instruction manual.
Only professional Haier technicians or Haier authorized after-sales service personnel should
install and maintain the incubator to prevent potential electric shock or fi re.
Be sure to place the incubator securely on solid and fl at surface. If the incubator is not secured
firmly or the installation place is inappropriate, the incubator may fall to cause damage or
personal injury.
This product cannot be installed on combustible surface.
Safety
Safety label
Safety Precautions
Check this manual in all cases marked with ,
to learn the nature of potential hazards and any
countermeasures that must be taken.
Failure to comply with the items under warning signs
may result in serious personal injury or even death.
Failure to comply with the items under precaution
signs may result in personal injury or damage to the
refrigerator and related property damage.
Actions that must be prohibited
Actions that must be followed
Warning
Caution
Elscolab BV.
Tolboomweg 10, 3784 XC Terschuur,
the Netherlands
Complied with the
regulation(EU)
2017/745 annex Ⅷ
Symbol for
“Medical device”

2
The incubator should only be operated by trained and authorized personnel.
Please use a dedicated and specifi ed power supply indicated on the nameplate of the incubator.
Potential fi re or electric shock may be resulted otherwise.
If the supply voltage is lower than 198V or higher than 264V, an automatic voltage regulator is
required, to ensure that the voltage meets the installation requirements.
If replacing the power cord, the cross-sectional area of the extension part shall not be less
than 2 mm² and the length shall not be longer than 3 m; Potential fi re or electric shock may be
resulted otherwise.
The power cord of this incubator is equipped with a three-prone plug and a standard three-prone
socket. Grounding pin of the power cord must not be cut or removed under any circumstances.
Be sure that the power plug and socket are tightly and reliably connected. Potential fire or
electric shock may be resulted otherwise.
Please use a power socket with a grounding wire to prevent electric shock. If the power socket
is not grounded, be sure to install the grounding wire by a professional technician.
The power cord of this incubator is properly grounded.
In case of leakage of fl ammable gas such as coal gas, close the gas supply valve. Open the
doors and windows for ventilation. Do not plug in or unplug the power plug of the incubator. The
action may cause explosion and fi re.
The pressure of the CO2 inlet can be adjusted to the range of 1.0 Bar ± 0.2 Bar. It cannot be
changed.
CO2 is a harmful gas that may cause harm to human health.
Only qualifi ed service personnel are allowed to operate the incubator's intake pipe, gas cylinder
or system that stores CO2 with appropriate tools.
Be sure that the CO2 concentration in the workplace does not exceed the safety limit.
Release of large amount of CO2 into a closed indoor space may cause suff ocation. In the case
of CO2 leakage, take safety measures immediately! Leave the room immediately and prevent
others from entering the room! Notify the security or fi re department!
When removing the plug from the power socket, hold the power plug tightly instead of pulling
the plug cord. Pulling the cord with hands may cause electric shock or fi re due to a potential
damage.
If the incubator does not operate normally, unplug the unit. Operating the incubator in an
abnormal state may cause electric shock or fi re.
Be sure to disconnect the power supply of the incubator before performing any repair or
maintenance to avoid potential electrical shock or personal injury.
Be sure to disconnect the CO2 gas supply to the incubator before performing any repair or
maintenance to avoid excessive indoor CO2 concentration caused by leakage.
Do not inhale medical fume or suspended particles inside and around the incubator during
maintenance as they may be harmful to your health.
When culturing tissues such as viruses and germs that are harmful to humans, please use the
incubator in a safe area with appropriate precautions. Improper installation may be harmful to
human health or the environment.
The incubator meets the requirements of the internationally recognized "Laboratory Biosafety
Manual", published by the World Health Organization in Geneva, which gives information on
decontaminants, their use, dilutions, properties and potential applications.

3
When the incubator is not in use for a long time, unplug the unit to prevent potential electric
shock, electric leakage or fi re due to aging of the plug cord.
If the incubator is left unused for a long time in an unsupervised area, make sure that no child
can get access to it and the door is not completely closed.
Decommissioning of an incubator shall be carried out by appropriate personnel. Dismantling the
door to avoid accidents such as suff ocation.
When restarting the incubator after power failure or power off, check the settings of the
incubator. Change of settings may lead to changes of the culture results.
Wear gloves when repairing it to avoid personal injury caused by sharp edges or corners.
When handling the incubator, make sure it does not fall down to avoid damage to the incubator
or personal injury.
Make sure no obstructions are around the incubator and keep good ventilation condition.
Any tissue, material or liquid substance that are flammable or may explode shall not be
used, otherwise the vapors emitted from them or fragments of explosives may release toxic
substances.
Do not place the incubator in a humid place or where may subject to splashing water. Otherwise,
it may cause electric leakage or electric shock due to compromised insulation.
Do not pour water directly onto the incubator, otherwise may cause electric shock or short
circuit.
Do not place any container with water or heavy objects on the incubator. If the object falls, it
may cause personal injury; or the spilled water may cause electric leakage or electric shock due
to compromised insulation.
Do not ground the incubator through a gas pipe, power hose, telephone line or lightning rod.
The above grounding methods can cause electric shock or other dangers.
Do not touch any electrical parts such as power plugs or any switches of the incubator with wet
hands, Electric shock may be resulted.
Users should not disassemble, repair or modify the incubator by themselves to avoid potential
fi re or personal injury due to improper operation.
Do not store fl ammable or explosive dangerous goods or volatile goods in the incubator, or use
fl ammable sprays near the incubator; Otherwise, it may cause explosion or fi re.
Do not put the packing plastic bag in a place that is accessible to a child, otherwise may cause
suff ocation accident.
Do not climb to the incubator or place objects on the incubator, otherwise the incubator may fall
and cause potential personal injury or damages on the incubator.
Do not insert metal objects such as nails or wires into any openings and gaps or any air vents
for internal air circulation on the incubator, as this may result in electric shock or injury due to
possibly contacting moving parts behind.
Please do not use the door handle when lifting, pulling or handling the incubator to avoid
damage to the incubator or personal injury.
Do not use electrical appliances in the working chamber of the incubator other than the models
recommended by the manufacturer.

4
Precautions for Use
1. Structure and components: this product include insulation box, heating system, control system
(including alarm system), data recording system (optional) and shelves with freely adjustable spacing.
2. The displayed temperature of the incubator is the value at the temperature sensing probe in it.
Although the displayed temperature sometimes diff ers from the actual value at center of the incubator,
it will gradually reach the actual temperature.
3. Clean the incubator with diluted neutral detergent. Do not use brushes, acids, gasoline, polishing
powder, polishing powder or hot water to clean the incubator. as these materials may damage painted
surfaces and plastic and rubber parts. Do not use volatile solvents such as gasoline to wipe plastic
and rubber parts.
4. The stainless steel parts inside the incubator are not acid-resistant, please take measure to
prevent. Never use acidic media in the incubator!
5. Unplug the power supply if the incubator is not in use for a long time.
6. When placing or taking samples, please minimize the the time of door opening to avoid fl uctuations
of temperature, humidity and CO2 concentration inside the incubator.
7. After opening the door, the temperature and CO2 concentration inside the incubator will change
sharply for a short time, which is normal; The temperature and CO2 concentration will generally
recover within 10 min after closing the door.
8. Do not open the door randomly under dry heat sterilization mode.
9. Pay attention to the calibration periods of the pressure reducing valve and pressure gauge.
10. User-prepared CO2 cylinders are pressurized vessels and must comply with relevant national
regulations and management specifi cations for pressure vessels.
11. Be sure to reserve certain clearance around the product, with a clearance of no less than its right-
side dimension. This space will easily cut off the power supply in an emergency.
12. After each test, dry the water in inner walls of the working chamber to avoid growth of bacteria.
13. The basic functions claimed by the electromagnetic compatibility test:
the basic function of incubator are: heating system working normally to realize temperature
control inside the incubator; controlling of CO2 concentration in the specifi ed range to realize CO2
concentration control inside the incubator;the data recording system (if any) working normally to
realize the data record transmission function; the electronic door lock (if any) working normally to
realize the corresponding function; buzzing or text display on the screen in the case of alarm.
14. Electromagnetic compatibility requiremen:
a) This machine complies with the emission and immunity requirements specifi ed in EN 61326-1;
b)This machine is designed and tested as Class A equipment specifi ed in CISPR:11. In domestic
situations, this machine may cause radio interference and protective measures shall be taken;
c) It is recommended to evaluate the electromagnetic environment before using the machine;
d)It is prohibited to use this machine near strong sources of radiation (such as unshielded video
sources), otherwise it may interfere with normal operation of this machine.

5
Product Description
1. Expected application
This product is a sophisticated laboratory instrument suitable for culture of biological cells, tissues
and bacteria used for biological experiments,which are widely used in the hospitals; research
laboratories; microbiological laboratories; sanitary-epidemiological surveillance service. It adopts
a fuzzy PID control algorithm to precisely control all parameters in the incubator to maximize the
culture success rate and culture effi ciency of biological cells and tissues. There are no absolute
contraindications to the product.
Caution The incubator must not be used for any purpose other than the expected function.
2. Product categories
This series of products is an directly-heated incubator.
3.Parameter control
The product is used to provide a culture environment with temperature range of ambient
temperature+3℃ ~ 55 ℃ , CO2 concentration range of 0 ~ 20% and relative humidity of > 90%. It
adopts a computer control for the temperature parameters and CO2 concentration parameter with
a digital display. The temperature display accuracy is 0.1 ℃ and the CO2 concentration display
accuracy is 0.1%.
4.Product structure
•The incubator body adopts table-type frame structure; the control circuit and the gas path are
installed behind the CO2 incubator, and the main power switch is set at left side facing to the
incubator body; the 7-inch color touch screen is installed on the front of the CO2 incubator door;
•The bottom of the working chamber adopts a water basin design, you can directly inject water into
the inner container by utilizing the inclined structure with high outer portion and low inner portion;
•The temperature control circuit is controlled by a microcomputer and is equipped with functions
such as automatic regulating characteristics and over temperature alarm. The incubator adopts
highprecision temperature sensor which greatly improves the control accuracy and reliability;
besides, it is also equipped with RS485 communication interface which making it possible to
realize remote control;
•The cabinet is built with a dual-door structure. The outer door is equipped with heating function to
ensure that there is no condensation on the inner glass door, making it convenient to observe the
product. At the same time, there is a door contact switch installed on the body behind the glass
door. When the glass door is opened, this switch can automatically close the CO2 solenoid valve
and turn off the fan provided for the gas circulation device, to avoid waste of CO2 or increase the
indoor CO2 concentration caused by release of CO2 into the air;
•Double-layer medical grade silicone sealing strip is used to ensure product sealing performance;
•For the convenience of users, there is a CO2 monitoring port on the glass door of the CO2
incubator for monitoring the CO2 concentration during use.
5.Safety system
•Multiple fault alarms: high/low temperature alarm, over temperature alarm, high/low CO2
concentration alarm, and door closing delay alarm;
•Multiple alarm modes: buzzing alarm, touch screen display alarm;
•All individual components are safely grounded.

6
6.CO2 concentration acquisit
The new type 190 ℃high temperature infrared (IR) sensor is based on the NDIR measurement
principle and adopts silicon MEMS emitter instead of conventional light source, and is able
complete more than 300 dry heat sterilization cycles. Its service life is up to 15 years.
7. Pollution control system
•There is a microbial high-efficiency filter is installed at the front end of the gas path, and the
gas will be fi ltered through it before entering the incubator to ensure the purity of CO2 ; the high-
effi ciency fi lter is placed outside the electric cabinet for easy replacement;
•There is a stainless steel sintered fi lter element installed on the back of the working chamber to
ensure that the gas inside the incubator is fi ltered before penetrating into the chamber;
•Dry heat sterilization at 180 ℃: Maintain the temperature of 180 ℃ for at least 1.5 hours. The
entire sterilization process takes only 12 hours from warming to cooling.
8.Humanized design
•Friendly touch-operated interface allows a sensitive response to finger movements with rubber
gloves;
•Announcement design, paperless offi ce message notepad function, and safety operation mode;
•The four corners of the liner is large arc-shaped, and the integrated stamping structure ensures
complete cleaning without dead spaces;
•Convenient drainage design;
•Integrated anti-skid shelf design; adjustable horizontal feet;
•Large capacity data storage with 15 years of data traceability.
We apology for the differences between the Haier CO2 incubator you received and the
diagrams in the user manual due to product improvement. The contents of this manual are
subject to changes without notice.

7
• Use a suitable unpacking tool such as scissors or wallpaper knife to cut the packing strap, Lift the
outer box vertically and move it horizontally; after that, check that if the accessories are complete.
Caution Avoid collision and scratches on the exterior of the incubator. For the sake of safety,
please have at least two adults for unpacking
• Since the incubator body is heavy, please choose suitable mechanical handling tools.
• Ambient temperature: 18 °C ~ 32 °C;
• Ambient humidity: ≤ 80% Rh;
• Atmospheric pressure: 86kPa~106kPa;
• Indoor use;
• Installation environment must be free from dust, vibration and corrosion gas;
• Avoid direct sunlight or sources of cold and heat;
• Make sure no corrosive substances or high concentration of dust around the incubator;
• Power voltage: Within rated voltage±10%;
• Voltage frequency: 50Hz.
• Altitude of the working position of the box:below 2000m.
• Pollution Degree:2.
• The transient state is Category II facility (overvoltage category).
• The safety of any system incorporating the equipment is the responsibility of the assembler of the
system.
•The incubator must have a space from the adjacent wall/object, as shown in the fi gure below.
• The operation of the incubator requires CO2 gas flow. Excessive CO2 can be
harmful to health. Therefore, the installation position shall have good ventilation
condition so that the exhaust gas from the rear wall can be quickly removed.
• The incubator shall not be placed in a place without a ventilation system. If there are
several incubators in the same room or the incubator is placed on the bottom layer of
the laboratory, an auxiliary ventilation system is recommended.
The expected performance of the incubator cannot be guaranteed when it is not used
in the specifi ed environment.
Product Installation
Product unpacking and handling
Installation environment
Spacing
For manual handling, be sure to have four adults (at least two) to lift the incubator
vertically with hands holding the bottom of the incubator, and then move it horizontally
to the position where the incubator is to be stored. Do not apply pressure on the outer
door of the incubator during handling. The door body may be damaged.
Do not block the pressure compensation hole on the rear wall. Do not place the
incubator in any position where it is diffi cult to operate the disconnect device.
Caution
Caution
Warning
Caution

8
The incubator has stack feet to stack two incubators together. The 4 stack feet are fi xed together
with built-in mechanical screws.
Stack installation
50mm
50mm
100mm
120mm

9
•Connect the gas cylinder with the pressure reducing valve, and then connect it to the incubator, as
shown below:
•The CO2 gas storage cylinders are provided by the user. The user may decide to
use an individual cylinder for gas supply or central gas supply. Safety use of CO2
gas storage cylinders and of central gas supply shall be operated in accordance with
relevant national standards.
•The CO2 gas filter can filter out particles of 0.2 um and greater with a filtration
effi ciency of more than 99.99%. It can withstand pressure of more than 3.5 Kg/cm2.
If any questions, please contact Haier or agencies designated by Haier for technical
support.
The gas purity shall be at least 99.5%. Do not use CO2 cylinders with carts.
CO2 gas source connection
• The corresponding interface is provided on the side of the electric cabinet on the back of the
incubator. There is hose supplied with the incubator. The gas entering the incubator must fi rst pass
through the pressure reducing valve to reduce the output pressure to 1.0 Bar ± 0.2 Bar. The pressure
reducing valve needs to be provided by user.
• From the perspective of technical safety, the output pressure may vary within the range of 1.0 Bar ±
0.2 Bar.
The pressure reducing valve is not a standard accessory for the incubator. Users may
purchase it through standard channels. Please choose pressure reducing valves
with stable quality, high resolution and stable pressure regulation performance. For
assistance, please contact Haier or agencies designated by Haier for technical support.
Installation and connection of gas source
Caution
CO2 gas storage cylinder
Filter
CO2 pressure
reducing valve
Caution
Warning

10
•Before connecting the main power supply, ensure that the power matches the power requirements
indicated on the nameplate. The requirements of the power supply for the incubator are:
Power voltage: AC 220-240V~；
Voltage frequency: 50Hz±1Hz；
•The accessories of the incubator include a power cable for grounding. Plug the quick connector of
the power cable into the lower right interface (facing the incubator) of the electric cabinet on the back
of the incubator.
Make sure that the power cable is connected in place. The power cable connector has
a self-locking structure. The main power supply unit shall be well grounded. Make sure
that the power cable of the incubator is properly grounded after connection.
Connection of the main power supply
Warning

11
Description of Parts ● Control Panel
Power switch
Stack foot
CO2 high effi ciency fi lter
Electric cabinet
Touch screen Horn
Rotating foot
Handle
Description of Parts
Sintered fi lter Remote alarm
Cable connector

12
Control panel
Door switch
Adjustable shelf
Outer door
CO2 detection port
Inner door handle
Drain connector Inner door

13
• The ambient temperature in the placement area of the incubator shall be at least 3~5 °C lower than
the control temperature;
• Always keep the outer door and the glass door opened;
• Inject distilled water into the reservoir (close to room temperature) and make sure the volume is
lower than the maximum limit of the bottom reservoir;
• Avoid overfl ow during water injection;
Water quality requirements: In order to ensure trouble-free operation of the incubator,
use sterile distilled water or equivalent water with the same quality level as sterilized
and distilled water. The acceptable conductivity shall be within the range of 1~20 μs/
cm. (The resistivity shall be within 50 kΩ ~ 1 megohm).
Please carefully observe or look up the instructions of the pressure reducing valve to
determine the regulating direction. Adjust the low-pressure adjusting knob properly for
a proper gas fl ow output to prevent a potential damage of the high effi ciency fi lter.
Presse the main power switch to ensure the power is properly connected.
Before operation, confi rm the output pressure of the low-pressure gauge is set properly. Open the
master valve of the cylinder counterclockwise. Observe the reading of the high-pressure gauge and
record the total C02 pressure in the high-pressure cylinder. Adjust the regulating screw clockwise
to open the low-pressure valve. Rotate the pressure regulating screw of the low-pressure gauge
clockwise to compress to open the valve. The high-pressure gas at the inlet is depressurized. The
low-pressure gas fl ow enters the working system through the outlet. The pressure of low-pressure
output gas shall be 1.0 Bar±0.2 Bar.
• Connect the main power supply;
• Turn on the main power switch;
• Language:Select the desired display language;
• Network: Search for the Internet and connect the unit to the wireless network;
• Time:You can set the time manually or synchronize with the Internet time (if connected to the
Internet);
• Temperature scale: Select the desired temperature standard °C or °F;
• Mode: Select the desired management mode. In normal mode, anyone can set and view the
parameters. In the authorization mode, only the administrator can set the parameters;
• Password: Set the administrator password for authority management; (Please keep the password in
a safe place. Contact Haier for technical support if you forget the password);
Commissioning and application
Preparation before use
Use distilled water
Open the pressure regulating valve on the CO2 gas storage cylinder
Power on
Initial setting for fi rst power-on
Caution
Caution
Caution

14
Language selection interface
Network settings interface
Time and temperature setting interface
For all display interfaces, the highlighted portion means that it is not selected. Click on
it and it will turn black, which means that it is selected.
Caution

15
Mode setting interface
Password setting interface
Home page
After completing the initial setup, enter the home page of the display screen.
1. Operation record: Time record and alarm record generated during operation of the incubator;
2. Operation data: Parameter and operation curve of the working chamber during operation of the
incubator;
3. Setting: Basic settings of the incubator;
4. Level: Water level display of the incubator, white for normal and red for water shortage;
5. Sterilization: Click to enter the sterilization setting;
6. Mute: Click to clear the buzzing alarm (the buzzing alarm continues when the set time is
over);
7. Message, notepad and announcement: Click to enter the message, notepad and
announcement interface.

16
Users can set parameters in two ways:
• Directly click the display parameter on the home page to enter the corresponding parameter settings
interface;
•Click the setting icon to enter the setting interface, and then select the culture condition setting to
enter the corresponding parameter setting;
The default setting temperature is 37.0 °C and the CO2 concentration is 5.0%.
Home page
Parameter settings

17
Culture condition setting interface
Basic settings
Click on the home page to enter the setting interface, and you can set the language, time and alarm
volume of the incubator by clicking the basic settings.
•Change language: Select the appropriate language in the drop-down menu;
•Time setting: Set the time in the drop-down menu;
•Alarm volume: In the event of an alarm, it can be used to adjust the alarm volume of the buzzer;
•Operation mode: Select normal mode or authorization mode according to your specifi c needs;
•Buzzing alarm delay time: In the event of a buzzing alarm, click the mute button on the home page; if
the alarm source still exist when the buzzing alarm delay time is over, the buzzing alarm will continue;
•Key volume: It can be used to adjust the key volume;
•Screen brightness: It can be used to adjust the display brightness of the screen;
•Screen sleep: It is suggested to set the screen sleep time according to the service time, so as to
extend the touch screen’s service life;
•Lock screen period: If no operations in the lock screen period, the screen will enter the sleep state.
•Door opening alarm:It can be set how long after the door is opened to alarm
•All changes will take eff ect after being saved by clicking.
•When creating a new user, you need to enter the card number and contact information.
The card number may be the user’s job number or other numbers.
Caution

18
Message, notepad, announcement
Click on the home page to enter the message, notepad and announcement interface.
•New message: Diff erent authorized users can send messages by creating a new message;
•Message box: After the user logs in, if there are unread messages, the number of unread message
will be highlighted on the home page icon, and the number of unread messages will be highlighted at
upper right corner of the message box;
•Notepad: It is used for recording. In normal mode, the notepad is open for view by anyone. In
authorization mode, the logged user can only see his/her own records;
•Announcement: It is used for multiple users. You can post the matters that you want to remind the
other users to the home page. When clicking the screen in sleep mode, the announcement interface
will be displayed fi rst, and then you may enter the home page.