Hanna Instruments HI 2209 User manual

Instruction Manual
HI2209 • HI22091
Bench pH Meters
www.hannainst.com

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Dear Customer,
Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using these
instruments. This manual will provide you with the necessary information
for the correct use of these instruments, as well as a precise idea of
their versatility.
If you need additional technical information, do not hesitate to e-mail
us at [email protected] or view our worldwide contact list at
www.hannainst.com.
All rights are reserved. Reproduction in whole or in part is prohibited
without the written consent of the copyright owner.

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PRELIMINARY EXAMINATION ........................................................4
GENERAL DESCRIPTION................................................................4
FUNCTIONAL DESCRIPTION...........................................................5
SPECIFICATIONS..........................................................................6
OPERATIONAL GUIDE...................................................................6
pH CALIBRATION.........................................................................8
pH BUFFER TEMPERATURE DEPENDENCE ...................................11
ELECTRODE CONDITIONING AND MAINTENANCE........................... 12
TEMPERATURE CORRELATION FOR pH SENSITIVE GLASS .............. 15
ACCESSORIES............................................................................16
ELECTRODE APPLICATION REFERENCE GUIDE...............................21
TABLE OF CONTENTS

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HI2209 bench pH meter is designed for simplicity of use.
It features a large easy-to-read liquid crystal display (LCD) and user
friendly keyboard.
The pH calibration is made simple through the easy-to-operate front
panel knobs for offset and slope adjustment.
A front knob is provided for easy manual set for temperature compen-
sation of the pH reading.
HI2209 can also measure ORP (Oxidation Reduction Potential) in
the mV range.
The range selection (pH, mV or °C for temperature compensation) is
made simple through the front membrane keyboard.
It is also available the model HI22091 with recorder output feature.
GENERAL DESCRIPTION
PRELIMINARY EXAMINATION
Remove the instrument from the packing material and examine
it carefully to make sure that no damage has occurred during
shipping. If there is any damage, please contact your local
Hanna Instruments Office.
Each meter is supplied complete with:
• HI1332B plastic-body combination double-junction refillable
pH electrode with BNC connector and 1 m (3.3') cable
•12 Vdc adapter
•Instruction manual
•Quality Certificate.
Note: Save all packing material until you are sure that the instru-
ment functions correctly. All defective items must be returned in
the original packing with the supplied accessories.

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FUNCTIONAL DESCRIPTION
1) Liquid Crystal Display (LCD)
2) Temperature setting knob
3) °C range selection key
4) mV range selection key
5) pH range selection key
6) pH offset adjustment knob
7) pH slope adjustment knob
8) Sample holders
9) Analog output connectors (HI22091 only)
10) BNC electrode connector
11) Reference electrode socket
12) Power adapter socket
13) ON/OFF switch
Front Panel
Rear Panel

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OPERATIONAL GUIDE
SPECIFICATIONS
RANGE 0.00 to 14.00 pH
±1999 mV
RESOLUTION 0.01 pH
1 mV
ACCURACY @ 25 °C ±0.01 pH
±1 mV
pH Calibration Manual, 2 point, through knobs
Temperature
Compensation
Manual from 0 to 100 °C
(32 to 212 °F)
pH Electrode
(included)
HI1332B plastic body, double junction,
refillable, with BNC and
1 m (3.3') cable
Analog Output
(HI22091 only)
0 to 5 V
0 to 14 pH/-1999 to + 1999 mV
Temperature: always 0
Input Impedance 1012 ohms
Power Supply 12 Vdc adapter (included)
Environment 0 to 50 °C (32 to 122 °F)
max. 95% RH non-condensing
Dimensions 235 x 222 x 109 mm
(9.2 x 8.7 x 4.3”)
Weight 1.3 Kg (2.9 lbs)
POWER CONNECTION
Plug the supplied 12 Vdc adapter into the power supply socket (#12
on page 5).
Note: Make sure the mains line is protected by a fuse.
ELECTRODE CONNECTION
For combination pH or ORP electrodes (with internal reference) connect
the electrode's BNC to the socket provided (#10 on page 5).
For an electrode with a separate reference, connect the measuring
electrode's BNC to the BNC socket (#10 on page 5) and the reference
electrode's jack to the socket provided (#11 on page 5).

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Note: To prevent damage to the electrode, remove the pH elec-
trode from the solution before turning the meter off.
If the meter is OFF, detach the electrode from the meter before
immersing the electrode in the storage solution.
TAKING pH MEASUREMENTS
Make sure that the instrument has been calibrated for
pH before taking pH measurements.
• Put the beakers (bottles 50 or 100 mL) with the
solutions under test on the sample holders.
• Switch the instrument on by pressing the ON/OFF
switch.
• Press the pH key to display the pH measurement.
• Immerse the electrode tip (4 cm / 1½") into the
sample and shake briefly.
• Take the temperature of the solution with a
ChecktempC or a glass thermometer (e.g.25 °C).
• Press and hold the Temp key to display tempera-
ture setting on the LCD and adjust the temperature
knob to display the temperature of the sample.
• Release Temp key. The meter returns to pH range.
• The display shows the pH value of the test solution compensated
for temperature.
If measurements are taken in different samples successively, it is recom-
mended that the electrode be rinsed thoroughly for better conditioning
and to eliminate cross-contamination of the sample.
For the rinsing process, it is recommended to use a liberal amount of
the next solution to be measured.

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pH CALIBRATION
• Connect the ORP electrode to the meter and
submerge the tip (4 cm / 1½") into the sample
to be tested.
Note: ORP measurements are taken without
temperature compensation.
• Press the mV key to enter the mV mode. Allow
a few minutes for the reading to stabilize.
• The display will indicate the mV value (positive
or negative).
AFTER MEASUREMENTS
• Press the ON/OFF switch to switch the instrument off.
IMPORTANT
The instrument's pH range should be re-calibrated:
• When the meter is new.
• Whenever the pH electrode is replaced.
• At least once a month.
• After use in aggressive chemicals.
• After cleaning procedure and changing the reference electrolyte.
• For greatest accuracy.
PREPARATION
Pour small quantities (up to 4 cm / 1½" level)
of pH7.01 (HI7007) and pH4.01 (HI7004) or
pH10.01 (HI7010) solution into clean beakers
(50 or 100 mL bottles). If possible use plastic
beakers to minimize any EMC interferences.
TAKING ORP MEASUREMENTS
HI2209 and HI22091 have the capability to take ORP measurements,
using an ORP electrode. Hanna Instruments offers a variety of ORP
electrodes for this purpose (see accessories). Contact your local Hanna
Instruments Office for more information.

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For accurate calibration, use two beakers for each buffer solution; the
first for rinsing the electrode, the second for calibration. In this way,
contamination of the buffer is minimized.
Note: The electrode should be submerged approximately
4 cm (1½") into the solution.
If you need to calibrate to NBS standards, use pH6.86 (HI7006)
and pH9.18 (HI7009) instead of pH7.01 and 10.01 respectively.
Use a ChecktempC or a glass thermometer as reference.
Put the beakers with the calibrations solutions on the beaker holders
on the top of the instrument.
PROCEDURE
• Switch the instrument on by pressing the
ON/OFF switch.
• Rinse and immerse the pH electrode into pH7.01
buffer and shake briefly. Wait for the reading to
stabilize.
• Note the temperature of the buffer solution using a
ChecktempC or a glass thermometer (e.g. 20 °C).
• Press and hold the Temp key to select temperature
setting.
• Adjust the Temperature knob until the LCD shows
the noted temperature.
• Release the Temp key. The instruments returns to
pH range.
If you are measuring in the acid range,
use pH4.01 as second buffer; if you are
measuring in the alkaline range, use
pH10.01 as second buffer.

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• Rinse and immerse the pH electrode in pH4.01 / pH10.01 buffer
and shake briefly.
• Wait a couple of minutes and adjust the Slope knob until display
shows the pH value at the noted temperature (see the pH versus
temperature chart).
• The pH calibration is now complete.
• Wait a couple of minutes and adjust the Offset knob until display
shows the pH value at the noted temperature (see the pH versus
temperature chart).

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pH BUFFER TEMPERATURE DEPENDENCE
Temperature has an effect on pH. The calibration buffer solutions are
affected by temperature changes to a lesser degree than normal
solutions.
For manual temperature calibration please refer to the following chart.
For instance, if the buffer temperature is 25 °C, the display should
show pH4.01, 7.01 or 10.01 at pH4, 7 or 10 buffers, respectively.
At 20 °C, the display should show pH4.00, 7.03 or 10.06. The meter
reading at 50 °C will then be 4.06, 6.98 or 9.82.
TEMP pH BUFFERS
°C °F 4.01 6.86 7.01 9.18 10.01
0 32 4.01 6.98 7.13 9.46 10.32
5 41 4.00 6.95 7.10 9.39 10.24
10 50 4.00 6.92 7.07 9.33 10.18
15 59 4.00 6.90 7.05 9.27 10.12
20 68 4.00 6.88 7.03 9.22 10.06
25 77 4.01 6.86 7.01 9.18 10.01
30 86 4.02 6.85 7.00 9.14 9.96
35 95 4.03 6.84 6.99 9.11 9.92
40 104 4.04 6.84 6.98 9.07 9.88
45 113 4.05 6.83 6.98 9.04 9.85
50 122 4.06 6.83 6.98 9.01 9.82
55 131 4.08 6.84 6.98 8.99 9.79
60 140 4.09 6.84 6.98 8.97 9.77
65 149 4.11 6.84 6.99 8.95 9.76
70 158 4.12 6.85 6.99 8.93 9.75
75 167 4.14 6.86 7.00 8.91 9.74
80 176 4.16 6.87 7.01 8.89 9.74
85 185 4.17 6.87 7.02 8.87 9.74
90 194 4.19 6.88 7.03 8.85 9.75
95 203 4.20 6.89 7.04 8.83 9.76

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ELECTRODE CONDITIONING
AND MAINTENANCE
Note: To prevent damage to the electrode, remove the pH elec-
trode from the solution before turning the meter off.
If the meter is OFF, detach the electrode from the meter before
immersing the electrode in the storage solution.
PREPARATION PROCEDURE
Remove the protective cap.
DO NOT BE ALARMED IF SALT DEPOSITS ARE PRESENT. This is normal
with electrodes and they will disappear when rinsed with water.
During transport tiny bubbles of air may form inside the glass bulb
affecting proper functioning of the electrode. These bubbles can be
removed by "shaking down" the electrode as you would do with a
glass thermometer.
If the bulb and/or junction is dry, soak the electrode in HI70300 storage
solution for at least one hour.

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For refillable electrodes:
If the fill solution (electrolyte) is more than 2½ cm (1") below the fill
hole, add HI7082 3.5M KCl electrolyte solution for double junction
or HI7071 3.5M KCl+AgCl electrolyte solution for single junction
electrodes. For a faster response, unscrew the fill hole screw during
measurements.
For AmpHel®electrodes:
If the electrode does not respond to pH changes, the battery is run
down and the electrode should be replaced.
MEASUREMENT
Rinse the electrode tip with distilled water. Immerse the tip (bottom
4 cm /1½") in the sample and stir gently for a few seconds.
For a faster response and to avoid cross contamination of the samples,
rinse the electrode tip with a few drops of the solution to be tested,
before taking measurements.
STORAGE
To minimize clogging and assure a quick response time, the glass
bulb and the junction should be kept moist and not allowed to dry
out. Replace the protective cap with a few drops of HI70300 storage
solution or, in its absence, electrolyte solution (HI7071 for single junc-
tion, or HI7082 for double junction electrodes). Follow the Preparation
Procedure above before taking measurements.
NEVER STORE THE ELECTRODE IN DISTILLED OR DEIONIZED WATER.
PERIODIC MAINTENANCE
Inspect electrode and cable. The cable used for connection to the meter
must be intact and there must be no points of broken insulation on
the cable or cracks on the electrode stem or bulb. Connectors must be
perfectly clean and dry. If any scratches or cracks are present, replace
the electrode. Rinse off any salt deposits.
For refillable electrodes:
Refill the reference chamber with fresh electrolyte (HI7071 for single
junction or HI7082 for double junction electrodes). Allow the electrode
to stand upright for 1 hour.
Follow the Storage Procedure above.

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CLEANING PROCEDURE
General Soak in HI7061 general cleaning solution for approxi-
mately 30 minutes.
Removal of films, dirt or deposits on the membrane/junction:
- Protein Soak in HI7073 protein cleaning solution for
15 min.
- Inorganic Soak in HI7074 inorganic cleaning solution for
15 minutes.
- Oil/grease Rinse with HI7077 Oil & Fat cleaning solution.
IMPORTANT: After performing any of the cleaning procedures rinse the
electrode thoroughly with distilled water, refill the reference chamber
with fresh electrolyte (not necessary for gel-filled electrodes) and soak
the electrode in HI70300 storage solution for at least 1 hour before
taking measurements.
TROUBLESHOOTING
Evaluate your electrode performance based on the following.
•Noise (Readings fluctuate up and down) could be due to:
• Clogged/Dirty Junction: Refer to the Cleaning Procedure above.
•Loss of shielding due to low electrolyte level (in refillable elec-
trodes only): refill with fresh HI7071 for single junction or HI7082
for double junction electrodes.
• Dry Membrane/Junction: soak in HI70300 storage solution for
at least 1 hour.
• Drifting: soak the electrode tip in warm (approx. 50-60 °C)
Hanna Instruments HI7082 solution for one hour and rinse the tip
with distilled water. Refill with fresh HI7071 for single junction
electrodes and HI7082 for double junction electrodes (refillable
electrodes only).
• Low Slope: refer to the cleaning procedure above.
• No Slope: check the electrode for cracks in glass stem or bulb and
replace the electrode.
• Slow Response/Excessive Drift: soak the tip in HI7061 solution
for 30 minutes, rinse thoroughly in distilled water and then follow
the Cleaning Procedure above.

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TEMPERATURE CORRELATION FOR
pH SENSITIVE GLASS
The resistance of glass electrodes partially depends on the temperature.
The lower the temperature, the higher the resistance. It takes longer
time for the reading to stabilize if the resistance is higher. In addition,
the response time will suffer to a greater degree at temperatures
below 10 °C.
Since the resistance of the pH electrode is in the range of 200 Mohm,
the current across the membrane is in the pico Ampere range. Large
currents can disturb the calibration of the electrode for many hours.
For these reasons high humidity environments, short circuits and static
discharges are detrimental to a stable pH reading.
The pH electrode's life also depends on the temperature. If constantly
used at high temperatures, the electrode life is drastically reduced.
Typical Electrode Life
Ambient Temperature 1- 3 years
90 °C Less than 4 months
120 °C Less than 1 month
High concentrations of sodium ions interfere with readings in alkaline
solutions; the pH at which the interference starts to be significant
depends upon the composition of the glass. This interference is the
alkaline error and causes the pH to be underestimated. Hanna
Instruments' glass formulations have the indicated characteristics.
Alkaline Error
Sodium Ion Correction for the Glass at 20-25 °C
0.1 Mol L-1 Na+
13.00 0.10
13.50 0.14
14.00 0.20
1.0 Mol L-1 Na+
12.50 0.10
13.00 0.18
13.50 0.29
14.00 0.40

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ACCESSORIES
pH CALIBRATION SOLUTIONS
HI70004P pH4.01 buffer solution, 20 mL sachet, 25 pcs.
HI70007P pH7.01 buffer solution, 20 mL sachet, 25 pcs.
HI70010P pH10.01 buffer solution, 20 mL sachet, 25 pcs.
HI7004L pH4.01 buffer solution, 500 mL
HI7006L pH6.86 buffer solution, 500 mL
HI7007L pH7.01 buffer solution, 500 mL
HI7009L pH9.18 buffer solution, 500 mL
HI7010L pH10.01 buffer solution, 500 mL
HI8004L pH4.01 buffer solution in FDA bottle, 500 mL
HI8006L pH6.86 buffer solution in FDA bottle, 500 mL
HI8007L pH7.01 buffer solution in FDA bottle, 500 mL
HI8009L pH9.18 buffer solution in FDA bottle, 500 mL
HI8010L pH10.01 buffer solution in FDA bottle, 500 mL
ELECTRODE STORAGE SOLUTIONS
HI70300L Storage solution, 500 mL
HI80300L Storage solution in FDA bottle, 500 mL
ELECTRODE CLEANING SOLUTIONS
HI70000P Electrode rinsing solution, 20 mL sachet, 25 pcs.
HI7061L General cleaning solution, 500 mL
HI7073L Protein cleaning solution, 500 mL
HI7074L Inorganic cleaning solution, 500 mL
HI7077L Oil & Fat cleaning solution, 500 mL
HI8061L General cleaning solution in FDA bottle, 500 mL
HI8073L Protein cleaning solution in FDA bottle, 500 mL
HI8077L Oil & Fat Cleaning solution in FDA bottle, 500 mL
ELECTROLYTE SOLUTIONS
HI7071 3.5M KCl + AgCl electrolyte solution, 4 x 30 mL, for
single junction electrodes
HI7072 1M KNO3electrolyte solution, 4 x 30 mL
HI7082 3.5M KCl electrolyte solution, 4 x 30 mL, for double
junction electrodes
HI8071 3.5M KCl + AgCl electrolyte solution in FDA bottle,
4 x 30 mL, for single junction electrodes
HI8072 1M KNO3electrolyte solution in FDA bottle, 4 x 30 mL
HI8082 3.5M KCl electrolyte solution in FDA bottle, 4 x 30 mL,
for double junction electrodes
ORP PRETREATMENT SOLUTIONS
HI7091L Reducing pretreatment solution, 500 mL
HI7092L Oxidizing pretreatment solution, 500 mL

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pH ELECTRODES
All electrodes part numbers ending in B are supplied with a BNC
connector and 1 m (3.3') cable.
HI1043B
Glass-body, double junction, refillable, combination pH electrode. Use:
strong acid/alkali.
HI1053B
Glass-body, triple ceramic, conic shape, refillable, combination pH
electrode. Use: emulsions.
HI1083B
Glass-body, micro, Viscolene, non-refillable, combination pH electrode.
Use: biotechnology, micro titration.
HI1131B
Glass-body, double junction, refillable, combination pH electrode.
Use: general purpose.
HI1330B
Glass-body, semimicro, single junction, refillable, combination pH
electrode. Use: laboratory, vials.

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HI1331B
Glass-body, semimicro, single junction, refillable, combination pH
electrode. Use: flasks.
HI1230B
Plastic-body (Ultem®), double junction, gel-filled, combination pH
electrode. Use: general, field.
HI2031B
Glass-body, semimicro, conic, refillable, combination pH electrode.
Use: semisolid products.
HI1332B
Plastic-body (Ultem®), double junction, refillable, combination pH
electrode. Use: general purpose.
FC100B
Plastic-body (PVDF), double junction, refillable, combination pH
electrode. Use: general purpose for food industry.
FC200B
Plastic-body (PVDF), open junction, conic, Viscolene, non-refillable,
combination pH electrode. Use: meat & cheese.

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FC911B
Plastic-body (PVDF), double junction, refillable with built-in amplifier,
combination pH electrode. Use: very high humidity.
FC210B
Glass-body, double junction, conic, Viscolene, non-refillable, combina-
tion pH electrode. Use: milk, yogurt.
FC220B
Glass-body, triple-ceramic, single junction, refillable, combination pH
electrode. Use: food processing.
HI1413B
Glass-body, single junction, flat tip, Viscolene, non-refillable, combi-
nation pH electrode. Use: surface measurement.
ORP ELECTRODES
HI3131B
Glass-body, refillable, combination platinum ORP electrode.
Use: titration.
HI3230B
Plastic-body (PES), gel-filled, combination platinum ORP electrode.
Use: general purpose.

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HI4430B
Plastic-body (PES), gel-filled, combination gold ORP electrode. Use:
general purpose.
EXTENSION CABLES FOR SCREW-TYPE ELECTRODES
(SCREW TO BNC ADAPTER)
HI7855/1 Extension cable 1m (3.3') long
HI7855/3 Extension cable 3m (9.9') long
HI7855/5 Extension cable 5m (16.5') long
HI7855/10 Extension cable 10m (33') long
HI7855/15 Extension cable 15m (49.5') long
OTHER ACCESSORIES
HI710005 Voltage adapter from 115 Vac to 12 Vdc
HI710006 Voltage adapter from 230 Vac to 12 Vdc
ChecktempC Pocket-size thermometer
(range -50.0 to 150.0 °C)
HI76405 Electrode holder
HI8427 pH and ORP electrode simulator with 1 m (3.3')
coaxial cable ending in female BNC connectors
HI931001
pH and ORP electrode simulator with LCD and
1 m (3.3') coaxial cable ending in female BNC
connectors