Hanna Instruments HI 4521 User manual

1
HI 4521 & HI 4522
pH/mV/ISE/Temperature/
Conductivity/Resistivity/TDS/Salinity
Bench Meters
Instruction Manual
www.hannainst.com
116
SALES AND TECHNICAL SERVICE CONTACTSSALES AND TECHNICAL SERVICE CONTACTS
SALES AND TECHNICAL SERVICE CONTACTSSALES AND TECHNICAL SERVICE CONTACTS
SALES AND TECHNICAL SERVICE CONTACTS
Australia:
Tel. [phone] • Fax [phone]
China:
Tel. [phone] • Fax [phone]
Egypt:
Tel. & Fax [phone]
Germany:
Tel. [phone] • Fax [phone]
Greece:
Tel. [phone] • Fax [phone]
Indonesia:
Tel. [phone] • Fax [phone]
Japan:
Tel. [phone] • Fax [phone]
Korea:
Tel. [phone] • Fax [phone]
Malaysia:
Tel. [phone] • Fax [phone]
Singapore:
Tel. [phone] • Fax [phone]
South Africa:
Tel. [phone] • Fax [phone]
Taiwan:
Tel. [phone] • Fax [phone]
Thailand:
Tel. [phone] • Fax [phone]
United Kingdom:
Tel. [phone] • Fax [phone]
USA:
Tel. [phone] • Fax [phone]
For e-mail contacts and a complete list of Sales and Technical offices, please see
www.hannainst.com .
MAN4521
10/06

2115
OTHER ACCESSORIES
HI 710005/8 Voltage adapter from 115 VAC / 12 VDC 800 mA (USA plug)
HI 710006/8 Voltage adapter from 230 VAC / 12 VDC 800 mA (European plug)
ChecktempC Pocket-size thermometer (range –50.0 to 150.0 ºC)
HI 76404N Electrode holder
HI 8427 pH and ORP electrode simulator with 1 m (3.3') coaxial cable ending in female BNC
connectors
HI 931001 pH and ORP electrode simulator with LCD and 1 m (3.3') coaxial cable ending in female BNC
connectors
HI 76312 Platinum 4-ring conductivity/TDS probe with temperature sensor and 1 m (3.3') cable
HI 7662-T Temperature probe with 1 m (3.3') cable
HI 92000 Windows®compatible software
HI 920010 9 to 9-pin RS232 cable
RECOMMENDATIONS FOR USERS
Before using these products, make sure they are entirely suitable for the environment in which they are used.
Operation of these instruments in residential areas could cause unacceptable interferences to radio and TV
equipment, requiring the operator to follow all necessary steps to correct interferences.
The glass bulb at the end of the pH electrode is sensitive to electrostatic discharges. Avoid touching this glass
bulb at all times.
During operation, ESD wrist straps should be worn to avoid possible damage to the electrode by electrostatic
discharges.
Any variation introduced by the user to the supplied equipment may degrade the instruments’ EMC
performance.
To avoid electrical shock, do not use these instruments when voltages at the measurement surface exceed
24 VAC or 60 VDC.
To avoid damage or burns, do not perform any measurement in microwave ovens.

ORP ELECTRODES
HI 3131B
Glass-body, refillable, combination platinum ORP electrode.
Use: titration.
HI 3230B
Plastic-body, gel-filled, combination platinum ORP electrode.
Use: general purpose.
HI 4430B
Plastic-body, gel-filled, combination gold ORP electrode.
Use: general purpose.
Consult the Hanna General Catalog for more electrodes with screw-type or BNC connectors.
EXTENSION CABLE FOR SCREW-TYPE ELECTRODES
(SCREW TO BNC ADAPTER)
HI 7855/1 Extension cable 1 m (3.3') long
HI 7855/3 Extension cable 3 m (9.9') long
3
HI 4521 and HI 4522 are warranted for two years against defects in workmanship and materials when used
for their intended purpose and maintained according to instructions. Electrodes and probes are guaranteed for
six months. This warranty is limited to repair or replacement free of charge.
Damage due to accidents, misuse, tampering or lack of prescribed maintenance is not covered.
If service is required, contact the dealer from whom you purchased the instrument. If under warranty, report
the model number, date of purchase, serial number and the nature of the failure. If the repair is not covered
by the warranty, you will be notified of the charges incurred. If the instrument is to be returned to Hanna
Instruments, first obtain a Returned Goods Authorization number from the Technical Service Department and
then send it with shipping costs prepaid. When shipping any instrument, make sure it is properly packed for
complete protection.
To validate your warranty, fill out and return the enclosed warranty card within 14 days from the date of
purchase.
Dear Customer,
Thank you for choosing a Hanna Instruments product. This manual will provide you with the necessary
information for correct use of the instrument.
Please read this instruction manual carefully before using the instrument.
If you need additional technical information, do not hesitate to e-mail us at [email protected] or see the
back side of this manual for our worldwide sales and technical service contacts.
These instruments are in compliance with directives.
WARRANTYWARRANTY
WARRANTYWARRANTY
WARRANTY
114

4
TABLE OF CONTENTSTABLE OF CONTENTS
TABLE OF CONTENTSTABLE OF CONTENTS
TABLE OF CONTENTS
WARRANTY ...................................................................................................................................... 3
PRELIMINARY EXAMINATION .............................................................................................................. 5
GENERAL DESCRIPTION ...................................................................................................................... 6
FUNCTIONAL DESCRIPTION ................................................................................................................ 7
SPECIFICATIONS ................................................................................................................................ 9
OPERATIONAL GUIDE ....................................................................................................................... 12
DISPLAYING MODES ......................................................................................................................... 14
SYSTEM SETUP ............................................................................................................................... 17
pH SETUP ...................................................................................................................................... 23
mV SETUP ..................................................................................................................................... 36
ISE SETUP (HI 4522 only) .............................................................................................................. 37
pH CALIBRATION ................................................................................................. 43
pH MEASUREMENT ................................................................................................. 47
mV & RELATIVE mV MEASUREMENTS ................................................................................................ 49
ISE CALIBRATION (HI 4522 only) ........................................................................ 51
ISE MEASUREMENT (HI 4522 only) ........................................................................ 55
CONDUCTIVITY SETUP ......................................................................................................................... 64
RESISTIVITY SETUP ............................................................................................................................. 79
TDS SETUP .............................................................................................................. 81
SALINITY SETUP .............................................................................................................. 83
CONDUCTIVITY CALIBRATION.................................................................................. 85
CONDUCTIVITY MEASUREMENT.................................................................................. 87
RESISTIVITY MEASUREMENT .................................................................................. 90
TDS MEASUREMENT ............................................................................................... 91
SALINITY CALIBRATION .................................................................................. 92
SALINITY MEASUREMENT .................................................................................. 92
TEMPERATURE CALIBRATION .................................................................................. 94
LOGGING ....................................................................................................................................... 95
PC INTERFACE .................................................................................................................................... 100
pH BUFFER TEMPERATURE DEPENDENCE .......................................................................................... 101
PROBE CONDITIONING & MAINTENANCE ..................................................................................... 102
ELECTRODE CONDITIONING & MAINTENANCE ..................................................................................... 103
TROUBLESHOOTING GUIDE ..............................................................................................................105
TEMPERATURE CORRELATION FOR pH SENSITIVE GLASS ...................................................................... 107
ACCESSORIES .................................................................................................................................. 108
113
FC 200B
Plastic-body, open junction, conic, Viscolene, non-refillable, combination pH electrode.
Use: meat & cheese.
FC 210B
Glass-body, double junction, conic, Viscolene, non-refillable, combination pH electrode.
Use: milk, yogurt.
FC 220B
Glass-body, triple-ceramic, single junction, refillable, combination pH electrode.
Use: food processing.
FC 911B
Plastic-body, double junction, refillable with built-in amplifier, combination pH electrode.
Use: very high humidity.

5
Remove the instrument from the packing material and examine it carefully to make sure that no damage has
occurred during shipping. If there is any damage, notify your dealer or the nearest Hanna Service Center.
The meters are supplied complete with:
•HI 1131B Glass-body Combination pH Electrode
•HI 76312 Four-ring Conductivity Probe with built-in temperature sensor and ID
•HI 7662-T Temperature probe
•pH 4.01 & 7.01 Buffer solutions, 20 mL each
•HI 700661 Cleaning solution, 2 x 20 mL
•HI 7071S Electrolyte solution
•HI 76404N Electrode Holder
•12 Vdc Power Adapter
•Instruction Manual
HI 4521 and HI 4522 are supplied with 12 Vdc/230 Vac adapter.
HI 4521-01 and HI 4522-01 are supplied with 12 Vdc/115 Vac adapter.
Note: Save all packing material until you are sure that the instrument works properly. Any defective item
must be returned in the original packing with the supplied accessories.
PRELIMINARY EXAMINATIONPRELIMINARY EXAMINATION
PRELIMINARY EXAMINATIONPRELIMINARY EXAMINATION
PRELIMINARY EXAMINATION
112
HI 2031B
Glass-body, semimicro, conic, refillable, combination pH electrode.
Use: semisolid products.
HI 1332B
Plastic-body, double junction, refillable, combination pH electrode.
Use: general purpose.
HI 1413B
Glass-body, single junction, flat tip, Viscolene, non-refillable, combination pH electrode.
Use: surface measurement.
FC 100B
Plastic-body, double junction, refillable, combination pH electrode.
Use: general purpose for food industry.

6
GENERAL DESCRIPTIONGENERAL DESCRIPTION
GENERAL DESCRIPTIONGENERAL DESCRIPTION
GENERAL DESCRIPTION
HI 4521 and HI 4522 are professional bench meters with color graphic LCD for pH, ORP (Oxidation Reduction
Potential), ISE (HI 4522 only), conductivity, resistivity, TDS, salinity and temperature measurements.
The display can be configured as a single channel or dual channel display in various modes: Basic information
only, GLP information, Graph and Log History mode.
The main features of the instruments are:
•Two input channels;
•Eight measurement parameters: pH, mV, ISE (HI 4522 only), conductivity, resistivity, TDS, salinity and
temperature;
•Manual selection, automatic and semiautomatic pH calibration in up to five points, with standard (1.68,
3.00, 4.01, 6.86, 7.01, 9.18, 10.01 and 12.45) and custom buffers (up to 5 custom buffers);
•Manual Selection and Custom Standard ISE calibration in up to five points, with standard (up to five standard
solutions for each measurement unit) and custom solutions (up to 5 custom solutions), with or without
temperature compensation (HI 4522 only);
•Pure water checking using the USP <645> standard;
•Conductivity probe automatic recognition;
•Automatic or custom standard conductivity calibration in up to four points, probe offset calibration;
•One fixed point salinity calibration (Percent Scale only);
•AutoHold feature to freeze first stable reading on the LCD;
•Two selectable alarm limits (for pH, mV, ISE, conductivity, resistivity, TDS, salinity);
•Three selectable logging modes: Automatic, Manual, AutoHold logging;
•Up to 100 logging lots for automatic or manual modes and up to 200 USP reports, up to 100 ISE methods
reports;
•Selectable area and sampling period feature for automatic logging;
•GLP feature;
•Online and offline graph;
•User-friendly interface on large color graphic LCD (240 x 320 pixels);
•Opto-isolated PC interface via RS232, respectively USB.
111
HI 1131B
Glass-body, single junction, refillable, combination pH electrode.
Use: general purpose.
HI 1330B
Glass-body, semimicro, single junction, refillable, combination pH electrode.
Use: laboratory, vials.
HI 1331B
Glass-body, semimicro, single junction, refillable, combination pH electrode.
Use: flasks.
HI 1230B
Plastic-body, double junction, gel-filled, combination pH electrode.
Use: general, field.

7
HI 4521/4522 DESCRIPTION
FRONT PANEL
REAR PANEL
1) Liquid Crystal Display (LCD)
2) Main Keyboard
3) USB connector
4) ON/OFF switch
5) Power adapter socket
6) RS232 serial communication connector
7) Conductivity probe connector
8) Temperature probe socket
9) BNC electrode connector for pH/ORP/Ion measurements
10) Reference input socket
FUNCTIONAL DESCRIPTIONFUNCTIONAL DESCRIPTION
FUNCTIONAL DESCRIPTIONFUNCTIONAL DESCRIPTION
FUNCTIONAL DESCRIPTION
110
pH ELECTRODES
All electrodes part numbers ending in B are supplied with a BNC connector and 1 m (3.3') cable, as shown
below:
HI 1043B
Glass-body, double junction, refillable, combination pH electrode.
Use: strong acid/alkali.
HI 1053B
Glass-body, triple ceramic, conic shape, refillable, combination pH electrode.
Use: emulsions.
HI 1083B
Glass-body, micro, Viscolene, non-refillable, combination pH electrode.
Use: biotechnology, micro titration.

8
KEYBOARD DESCRIPTION
FUNCTION KEYS
VIRTUAL KEYS
The upper row keys are assigned to the virtual keys placed on the bottom of the LCD, which allow you to
perform the displayed function, depending on the current menu (e.g. , and in
Measure mode).
Note:All the virtual keys are assigned to the focused channel (with key exception).
LCD GENERAL DESCRIPTION
To enter/exit calibration mode;
To select the desired measurement mode, pH, mV, Rel mV,ISE (HI 4522 only), Conductivity,
Resistivity, TDS, Salinity;
To enter Setup (System Setup, pH Setup, mV Setup, ISE Setup (HI 4522 only), Conductivity
Setup, Resistivity Setup , TDS Setup or Salinity Setup) and to access Log Recall function;
To obtain general information about the selected option / operation.
109
HI 7035M 111800 µS/cm (µmho/cm), 230 mL bottle
HI 7035L 111800 µS/cm (µmho/cm), 500 mL bottle
HI 8035L 111800 µS/cm (µmho/cm), 500 mL FDA approved bottle
HI 7037L 100% NaCl sea water standard solution, 500 mL
ELECTRODE STORAGE SOLUTIONS
HI 70300L Storage Solution, 500 mL bottle
HI 80300L Storage Solution in FDA approved bottle, 500 mL
ELECTRODE AND PROBE CLEANING SOLUTIONS
HI 70000P Electrode Rinse Sachets, 20 mL, 25 pcs
HI 7061L General Purpose Solution, 500 mL bottle
HI 7073L Protein Cleaning Solution, 500 mL bottle
HI 7074L Inorganic Cleaning Solution, 500 mL bottle
HI 7077L Oil & Fat Cleaning Solution, 500 mL bottle
HI 8061L General Purpose Solution in FDA approved bottle, 500 mL
HI 8073L Protein Cleaning Solution in FDA approved bottle, 500 mL
HI 8077L Oil & Fat Cleaning Solution in FDA approved bottle, 500 mL
ELECTRODE REFILL ELECTROLYTE SOLUTIONS
HI 7071 3.5M KCl + AgCl Electrolyte, 4x30 mL, for single junction electrodes
HI 7072 1M KNO3Electrolyte, 4x30 mL
HI 7082 3.5M KCl Electrolyte, 4x30 mL, for double junction electrodes
HI 8071 3.5M KCl + AgCl Electrolyte in FDA approved bottle, 4x30 mL, for single junction electrodes
HI 8072 1M KNO3Electrolyte in FDA approved bottle, 4x30 mL
HI 8082 3.5M KCl Electrolyte in FDA approved bottle, 4x30 mL, for double junction electrodes
HI 8093 1M KCl + AgCl Electrolyte in FDA approved bottle, 4x30 mL
ORP PRETREATMENT SOLUTIONS
HI 7020L Test Solution 200-275 mV, 500 mL bottle
HI 7021L Test Solution 240 mV, 500 mL bottle
HI 7022L Test Solution 470 mV, 500 mL bottle
HI 7091L Reducing Pretreatment Solution, 500 mL
HI 7092L Oxidizing Pretreatment Solution, 500 mL

9
SPECIFICATIONSSPECIFICATIONS
SPECIFICATIONSSPECIFICATIONS
SPECIFICATIONS
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ACCESSORIESACCESSORIES
ACCESSORIESACCESSORIES
ACCESSORIES
pH BUFFER SOLUTIONS
HI 6001 pH 1.679 Buffer Solution, 500 mL bottle
HI 6003 pH 3.000 Buffer Solution, 500 mL bottle
HI 8004L pH 4.01 Buffer Solution in FDA approved bottle, 500 mL
HI 6004 pH 4.010 Buffer Solution, 500 mL bottle
HI 8006L pH 6.86 Buffer Solution in FDA approved bottle, 500 mL
HI 6068 pH 6.862 Buffer Solution, 500 mL bottle
HI 8007L pH 7.01 Buffer Solution in FDA approved bottle, 500 mL
HI 6007 pH 7.010 Buffer Solution, 500 mL bottle
HI 6091 pH 9.177 Buffer Solution, 500 mL bottle
HI 8009L pH 9.18 Buffer Solution in FDA approved bottle, 500 mL
HI 8010L pH 10.01 Buffer Solution in FDA approved bottle, 500 mL
HI 6010 pH 10.010 Buffer Solution, 500 mL bottle
HI 6124 pH 12.450 Buffer Solution, 500 mL bottle
CONDUCTIVITY BUFFER SOLUTIONS
HI 70033P 84 µS/cm (µmho/cm), 20 mL sachets (25 pcs.)
HI 7033M 84 µS/cm (µmho/cm), 230 mL bottle
HI 7033L 84 µS/cm (µmho/cm), 500 mL bottle
HI 8033L 84 µS/cm (µmho/cm), 500 mL FDA approved bottle
HI 70031P 1413 µS/cm (µmho/cm), 20 mL sachets (25 pcs.)
HI 7031M 1413 µS/cm (µmho/cm), 230 mL bottle
HI 7031L 1413 µS/cm (µmho/cm), 500 mL bottle
HI 8031L 1413 µS/cm (µmho/cm), 500 mL FDA approved bottle
HI 70039P 5000 µS/cm (µmho/cm), 20 mL sachets (25 pcs.)
HI 7039M 5000 µS/cm (µmho/cm), 230 mL bottle
HI 7039L 5000 µS/cm (µmho/cm), 500 mL bottle
HI 8039L 5000 µS/cm (µmho/cm), 500 mL FDA approved bottle
HI 70030P 12880 µS/cm (µmho/cm), 20 mL sachets (25 pcs.)
HI 7030M 12880 µS/cm (µmho/cm), 230 mL bottle
HI 7030L 12880 µS/cm (µmho/cm), 500 mL bottle
HI 8030L 12880 µS/cm (µmho/cm), 500 mL FDA approved bottle
HI 7034M 80000 µS/cm (µmho/cm), 230 mL bottle
HI 7034L 80000 µS/cm (µmho/cm), 500 mL bottle
HI 8034L 80000 µS/cm (µmho/cm), 500 mL FDA approved bottle

10
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107
TEMPERATURE CORRELATIONTEMPERATURE CORRELATION
TEMPERATURE CORRELATIONTEMPERATURE CORRELATION
TEMPERATURE CORRELATION
FORFOR
FORFOR
FOR pp
pp
pH SENSITIVE GLASSH SENSITIVE GLASS
H SENSITIVE GLASSH SENSITIVE GLASS
H SENSITIVE GLASS
Typical Electrode Life
Ambient Temperature 1 – 3 years
90 ºC (194 ºF) Less than 4 months
120 ºC (248 ºF) Less than 1 month
Alkaline Error
High concentrations of sodium ions interfere with readings in alkaline solutions. The pH at which the interference
starts to be significant depends upon the composition of the glass. This interference is called alkaline error and
causes the pH to be underestimated. Hanna’s glass formulations have the indicated characteristics.
1.0 Mol L-1 Na+
0.1 Mol L-1 Na+
Sodium Ion Correction for the Glass at 20-25 ºC (68-77 ºF)
Concentration pH Error
13.00
13.50
14.00
12.50
13.00
13.50
14.00
0.10
0.14
0.20
0.10
0.18
0.29
0.40
The resistance of glass electrodes partially depends on the temperature. The lower the temperature, the higher the
resistance. It takes more time for the reading to stabilize if the resistance is higher. In addition, the response time
will suffer to a greater degree at temperatures below 25 ºC (77 ºF).
Since the resistance of the pH electrode is in the range of 50 – 200 Mohm, the current across the membrane is in the pico
Ampere range. Large currents can disturb the calibration of the electrode for many hours.
For these reasons high humidity environments, short circuits and static discharges are detrimental to a stable
pH reading.
The pH electrode’s life also depends on the temperature. If constantly used at high temperatures, the
electrode life is drastically reduced.