Hanna Instruments HI 84185 User manual

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Instruction Manual
HI 84185
ISE Ammonia Nitrogen
Meter
www.hannainst.com

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All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the
copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895 , USA.
Dear Customer,
Thank you for choosing a Hanna product. This manual will provide you with the necessary
information for the correct use of the instrument. Please read it carefully before using the meter.
If you need additional technical information, do not hesitate to e-mail us at [email protected].
This instrument is in compliance with directives.
TABLE OF CONTENTS
PRELIMINARY EXAMINATION................................................................................................. 3
GENERAL DESCRIPTION ........................................................................................................ 4
SPECIFICATIONS................................................................................................................... 5
PRINCIPLE OF OPERATION .................................................................................................... 6
FUNCTIONAL DESCRIPTION ................................................................................................... 7
START UP ............................................................................................................................ 8
GUIDE TO DISPLAY CODES ..................................................................................................... 9
TIPS FOR AN ACCURATE MEASUREMENT ............................................................................. 16
MEASUREMENT PROCEDURE .............................................................................................. 16
FUSE REPLACEMENT .......................................................................................................... 18
ELECTRODE CONDITIONING AND MAINTENANCE .................................................................. 18
ACCESSORIES .....................................................................................................................22
WARRANTY........................................................................................................................23
MODEL IDENTIFICATION
Two models are available:
HI 84185-01 - 115 Vac power supply
HI 84185-02 - 230 Vac power supply
WARRA (NTY

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PRELIMINARY EXAMINATION
Please examine this product carefully. Make sure that the instrument is not damaged. If any
damage occurred during shipment, please notify your Dealer.
Each HI 84185 instrument is supplied complete with:
• Reagents set for 20 tests
• One 1000 µL automatic pipette
• Six plastic tips for the 1000 µL automatic pipette
• Two 50 mL beakers
• HI 61101 Ammonia electrode
• HI 7662-T Temperature probe
• Stir bar (2 pcs.)
• Power cable
• HI 4001-40 Refilling solution (4×30 mL)
• HI 4001-51 Replacement membrane (10 pcs.)
• One HI 4000-47-4 and one HI 4000-47-7 pH 4 & 7 powder packets
• One 1 mL syringe
• One 1 mL pipette
• Instruction sheet
• Instruction manual
• One pair of tweezers
Note:Save all packing material until you are sure that the instrument works correctly.
Any defective item must be returned in its original packing.

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GENERAL DESCRIPTION
SIGNIFICANCE OF USE
The nitrogenous compounds of must and wine are deriving from grapes and play important role in
fermentation, clarification, and potential microbial instability of wines. They are profoundly modified
during the alcoholic fermentation by the physiologic activity of yeast. Thus, yeast assimilates
60-70% of the must nitrogen, ammonium ion completely disappearing during the fermentation
and the total nitrogen being slightly reduced.
Ammonia is present in grapes as ammonium ion in a few milligrams amount and it serves as the
primary form of available nitrogen for yeast metabolism. So, the content of ammonium ion can
drastically decrease during the alcoholic fermentation, increasing again, especially in red wines, at the
end of the malolactic fermentation because the lactic bacteria release ammonia nitrogen in wine.
The amount of ammonium ion in must influences the rapidity of fermentation start and evolution.
The ammonia concentration ranges from 24 to 209 mg/L (ppm) in grapes and from a few mg/L
(ppm) to about 50 mg/L (ppm) in wine.
The HI 84185 ISE Ammonia Nitrogen meter measures the ammonia nitrogen (N-NH3) content in
wine using an ion selective electrode. The used method is double standard addition, a simple and
rapid method of analysis, and the result is readily displayed in ammonia nitrogen (N-NH3) mg/L
(ppm).
The HI 84185 is a low cost, easy to use, ammonia nitrogen (N-NH3) ISE instrument that performs
automatic analysis with all the necessary calculations assuring to the user a simple and effective
interface.
The instrument comes with a powerful and effective built-in algorithm to analyse the shape of the ISE
electrode response and to determine the reaction completion.
By simply pressing the Start key, the instrument performs automatic analysis, all the necessary
calculations and verifications. The result is immediately displayed in convenient units, then the
instrument is ready for another measurement.

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Range 0 to 50 mg/L (ppm) N-NH3
Resolution 1 mg/L (ppm) N-NH3
Accuracy ±5% of reading
Sample volume 50 mL
Temperature Compensation Automatic from 0 to 80 °C
Electrode HI 61101 Ammonia combined ISE
Temperature Probe HI 7662-T (included)
LOG feature 50 samples
Stirring speed 500 rpm
Environment 0 to 50 °C (32 to 122 °F); max 95% RH non-condensing
Power supply 220V/50Hz; 10VA
Dimensions 208 x 214 x 163 mm (8.2 x 8.4 x 6.4") (with beaker)
Weight 2200 g (77.6 oz.)
REQUIRED REAGENTS
Code Description Quantity/Test
HI 84185-0 ISA alkaline reagent 1 mL
HI 84185-1 Standard 1 1 mL
HI 84185-2 Standard 2 1 mL
SPECIFICATIONS

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PRINCIPLE OF OPERATION
The determination of ammonia nitrogen concentration is made according to double standard addition
method. This technique enables an unknown concentration and the electrode’s slope factor to be
determined simultaneously.
The Automatic Temperature compensation is available using HI 7662-T temperature probe. By
setting the electrode isopotential point and enabling temperature compensation option in Setup
menu, the obtained concentration is temperature compensated.
The accuracy of the obtained result depends on the stability of the mV reading in every step of the
measurement.
Three selectable stability criteria can be selected in Setup menu. Select Medium or Accurate
criteria in order to have accurate analysis.

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FUNCTIONAL DESCRIPTION
FRONT PANEL
REAR PANEL
INSTRUMENT DESCRIPTION
1) Graphic LCD
2) Functional keys
3) Keypad
4) Electrode holder
5) Ammonia electrode 10) BNC electrode connector
6) Temperature probe 11) Power switch
7) Electrode holder 12) Fuse
8) Reference socket 13) Power cable connector
9) Temperature probe socket

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KEYPAD DESCRIPTION
1) ESC - to leave the current screen and to return either to the previous screen or to the
main screen. In the setup parameter modifying mode by pressing ESC the value is
not modified.
2) - to modify a parameter value, to scroll the information from the instrument’s setup
3) HELP - to access/leave the instrument’s contextual help
4) - to modify a parameter value, to scroll the information from the instrument’s setup
5) Functional Keys
START UP
• Place the instrument on a flat table. Do not place the instrument in direct sunlight.
• Connect the instrument to mains socket with ground connection and the correct voltage and
frequency. See the label on the instrument rear for this.
• Connect the HI 61101 ammonia electrode and the HI 7662-T temperature probe.
• Turn the instrument ON using the power switch from the rear panel of the instrument and
wait until main screen is displayed.

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When the input reading is unstable.
When the stirrer is on.
Temperature warning (only if the “Temperature compensation” is disabled). The
warning symbol will be displayed if the temperature difference between the temperature of
the sample confirmed at the first step and the subsequent readings is greater than 3 °C.
Main screen display
Press Setup to acces the instrument’s setup.
Press RCL to view logged data.
Press Start to begin a new measurement cycle.
Press HELP at any moment to view the contextual help.
Measure screens
Icons from the stirrer and reading status area
GUIDE TO DISPLAY CODES
During the instrument’s operation a series of informations are displayed on the LCD.
1) current time information area
2) instrument status area
3) functional keys area
4) stability criteria
5) stirrer and reading status area
Conditioning screen
A two minutes conditioning period is initiated.
Press Continue to stop this conditioning period and skip the
message.
Measure screen
Press ESC at any moment during the measurement cycle and
the instrument will return to the main screen.
Press Pause to pause the measurement process.
Press Stir to start/stop the stirrer.

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Add standard screen
Add the corresponding standard. Press Read to start the next
step analysis if “Reading confirm” option is enabled in the
Setup menu. If “Reading confirm” option is disabled, the
instrument will start with the next step analysis.
Wrong standard error screen
This screen is displayed if step 2 and 3 of the current reading is
out of ±2000mV range or if the difference between the stable
readings from the current and the previous step is to small
(±3mV) or, if in auto confirm mode, the standard addition is
not detected.
Wrong input error screen
This screen is displayed if the current reading is out of
±2000mV range.
If the difference between the temperature of the sample
confirmed at the first step and the subsequent readings is
greater than 3°C and the temperature compensation feature is
disabled in Setup, the temperature warning icon will blink
and the message “WARNING: Large temperature change!”
will be displayed.
Sample reading confirm screen (only if this feature is enabled
in setup)
Press CFM to memorize the reading.
Pause screen
Press Resume to continue the measurement process.
Press Stir to start/stop the stirrer.