Hanna Instruments HI 96801 User manual

HI96801
Refractometer for Sucrose
Measurements
INSTRUCTION MANUAL

Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using
this instrument.
This manual will provide you with the necessary information
for correct use of this instrument, as well as a precise idea
of its versatility.
If you need additional technical information, do not hesitate
to e‑mail us at [email protected] or view our worldwide
contact list at www.hannainst.com.
Dear
Customer,
All rights are reserved. Reproduction in whole or in part is prohibited
without the written consent of the copyright owner, Hanna Instruments
Inc., Woonsocket, Rhode Island, 02895, USA.

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TABLE OF CONTENTS
Preliminary Examination .......................................................
General Description...............................................................
Specifications.......................................................................
Principle of Operation...........................................................
Functional Description...........................................................
Display Elements..................................................................
Measurement Guidelines .......................................................
Calibration Procedure............................................................
Measurement Procedure........................................................
Making a Standard % Brix Solution ......................................
To Change Temperature Unit .................................................
Battery Replacement.............................................................
Error Messages.....................................................................
Accessories...........................................................................
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Remove the instrument from the packing materials and examine carefully
to ensure no damage has occurred during shipping. If any damage has
occurred, contact your local Hanna Instruments Office.
Each HI96801 instrument is supplied with:
• 9 V battery
• Instruction manual
• Plastic pipette
• Quality Certificate
Note: Save all packing material until you are sure that the instrument
functions correctly. A defective instrument must be returned in its
original packing.
PRELIMINARY EXAMINATION

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The HI96801 Digital Sucrose Refractometer is a rugged portable, water
resistant device that benefits from Hanna Instruments’ years of experience
as a manufacturer of analytical instruments.
The HI96801 is an optical instrument that employs the measurement of
refractive index to determine the % Brix of sugar in aqueous solutions. The
method is both simple and quick. Samples are measured after a simple user
calibration with deionized or distilled water. Within seconds the instrument
measures the refractive index of the sample and converts it to % Brix
concentration units.
TheHI96801digitalrefractometereliminatestheuncertainityassociatedwith
mechanicalrefractometersandiseasilyportableformeasurementsinthefield.
Themeasurementtechniqueandtemperaturecompensationemploymethodo‑
logy recommended in the ICUMSA Methods Book (International Commission
for Uniform Methods of Sugar Analysis).
Temperature (in °C or °F) is displayed simultaneously with the measurement
on the large dual level display along with icons for Low Power and other
helpful message codes.
Key features include:
• Dual‑level LCD
• Automatic Temperature Compensation (ATC)
• Easy setup and storage
• Battery operation with Low Power indicator (BEPS)
• Automatically turns off after 3 minutes of non‑use.
GENERAL DESCRIPTION

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SPECIFICATIONS
Range 0.0 to 85.0 %Brix / 0.0 to 80.0 °C
(32.0 to 176.0 °F)
Resolution 0.1 %Brix / 0.1 °C (0.1 °F)
Accuracy ± 0.2 %Brix /
± 0.3 °C (±0.5 °F)
Temperature Compensation Automatic between 10.0 and 40.0 °C
(50.0 ‑ 104.0 °F)
Measurement Time Approximately 1.5 seconds
Minimum Sample Volume 100 µL (cover prism totally)
Light Source Yellow LED
Case Material ABS
Enclosure Rating IP65
Battery Type / Life 9V / 5000 readings
Auto‑Off After 3 minutes of non‑use
Dimensions 192 x 102 x 69 mm (7.6 x 4.1x 2.7”)
Mass 350 g (12.3 oz)

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In the HI96801, light from an LED passes through a prism in contact with the
sample. An image sensor determines the critical angle at which the light is
no longer refracted through the sample. The HI96801 automatically applies
temperature compensation to the measurement and converts the refractive
indexofthesampletosucroseconcentrationinunitsofpercent(byweight)Brix.
TheBrix determinationismadebymeasuringtherefractive indexofasolution.
Refractive Index is an optical characteristic of a substance and the number of
dissolved particles in it. Refractive Index is defined as the ratio of the speed
of light in empty space to the speed of light in the substance. A result of this
property is that light will “bend”, or change direction, when it travels through
a substance of different refractive index. This is called refraction.
When passing from a material with a higher to lower refractive index, there
is a critical angle at which an incoming beam of light can no longer refract,
but will instead be reflected off the interface. The critical angle can be used
to easily calculate the refractive index according to the equation:
sin (θcritical) = n2/ n1
Where n2is the refractive index of the lower‑density medium; n1is the
refractive index of the higher‑density medium.
PRINCIPLE OF OPERATION

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1. Liquid Crystal Display (LCD)
2. Secondary Display
3. Primary Display
4. READ Key (User Measurement)
5. ZERO Key (User Calibration)
6. ON/OFF
7. Stainless Steel Sample Well and Prism
TOP VIEW
FUNCTIONAL DESCRIPTION
8. Battery Cover
9. Battery Compartment
BOTTOM

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1. Battery (blinks when low battery condition detected)
2. Primary Display (displays measurement and error messages)
3. Measurement in Progress Tag
4. SETUP: Factory Calibration Tag
5. CAL: Calibration Tag
6. Measurement Units
7. Automatic Temperature Compensation
(blinks when temperature exceeds 10.0‑40.0 °C /50.0‑104.0 °F range)
8. Temperature Units
9. Secondary Display (displays temperature measurements; when blinking,
temperature has exceeded operation range: 0.0‑80.0 °C/32.0‑176.0 °F)
DISPLAY ELEMENTS

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• Handle instrument carefully. Do not drop.
• Do not immerse instrument under water.
• Do not spray water to any part of instrument except the “sample well”
located over the prism.
• The instrument is intended to measure Sucrose solutions. Do not expose
instrument or prism to solvents that will damage it. This includes most
organic solvents and extremely hot or cold solutions.
• Particulate matter in a sample may scratch the prism. Absorb sample
with a soft tissue and rinse sample well with deionized or distilled water
between samples.
• Use plastic pipettes to transfer all solutions. Do not use metallic tools
such as needles, spoons or tweezers as these will scratch the prism.
• To reduce the effects of evaporation or absorption of water when taking
readings over a period of time, the prism and sample well can be covered
with plastic wrap.
MEASUREMENT GUIDELINES