Hanna Instruments HI 96753C User manual

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Instruction Manual
HI 96753C
Chloride ISM
99 Washington Street
Melrose, MA 02176
Phone [phone]
Toll Free 1-800-517-8431
Visit us at www.TestEquipmentDepot.com

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TABLE OF CONTENTS
PRELIMINARY EXAMINATION ..............................................................................................3
GENERAL DESCRIPTION .....................................................................................................4
ABBREVIATIONS ................................................................................................................4
SPECIFICATIONS .................................................................................................................5
PRECISION AND ACCURACY ................................................................................................5
PRINCIPLE OF OPERATION .................................................................................................6
FUNCTIONAL DESCRIPTION ................................................................................................7
ERRORS AND WARNINGS ..................................................................................................9
GENERAL TIPS FOR AN ACCURATE MEASUREMENT ...........................................................11
STARTUP .........................................................................................................................12
MEASUREMENT PROCEDURE ...........................................................................................12
VALIDATION PROCEDURE .................................................................................................15
CALIBRATION PROCEDURE ...............................................................................................17
GLP .................................................................................................................................20
BATTERY MANAGEMENT ..................................................................................................21
BATTERY REPLACEMENT ..................................................................................................22
ACCESSORIES ...................................................................................................................22
WARRANTY ......................................................................................................................23
Dear Customer,
Thank you for choosing a Hanna product. This manual will provide you with the necessary
information for the correct use of the instrument. Please read it carefully before using the meter.
All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner,
Hanna Instruments Inc., Woonsocket, Rhode Island, 02895 , USA.
PRELIMINARY EXAMINATION
Please examine this product carefully. Make sure that the instrument is not damaged. If any
damage occurred during shipment, please notify your Dealer.
Each HI 96753 Ion Selective Meter is supplied complete with:
• Two Sample Cuvettes and Caps
• CAL CHECK standard cuvettes
• 9V Battery
• Cloth for wiping cuvettes
• Instrument quality certificate
• Instruction Manual
• Rigid carrying case
Note:Save all packing material until you are sure that the instrument works correctly. Any
defective item must be returned in its original packing.

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GENERAL DESCRIPTION
ABBREVIATIONS
SPECIFICATIONS
PRECISION AND ACCURACY
The HI 96753 is an auto diagnostic portable microprocessor meter that benefits from Hanna’s
years of experience as a manufacturer of analytical instruments. It has the advanced optical
system based on a special Light Emitting Diode (LED) and a narrow band interference filter that
allows most accurate and repeatable readings. All instruments are factory calibrated and the
electronic and optical design minimizes the need of frequent calibration.
With the powerful CAL CHECKTM validation function, you are able to validate good performance
of your instrument at any time. The validation procedure is extremely user friendly. Just use the
exclusive HANNA ready-made, NIST traceable standards to verify the performance of the
instrument and recalibrate if necessary.
All instruments are splash waterproof and the LED and filter units are protected from dust or dirt
by a transparent cup. This makes the instruments fulfill field applications. Display messages aid
the user in routine operation. The meter has an auto-shut off feature that will turn off the
instrument after 10 minutes of non use in measurement mode or after 1 hour if left in
calibration mode .
The meter uses an exclusive positive-locking system to ensure that the cuvette is in the same
position every time it is placed into the measurement cell. It is designed to fit a cuvette with a
larger neck making it easier to add both sample and reagents. The cuvette is made from special
optical glass to obtain best results.
The HI 96753 meter measures the chloride (Cl–) content in water and wastewater samples in
the 0.0 to 20.0 mg/L (ppm) range. The method is an adaptation of the mercury(II) thiocyanate
method.
The reagents are in liquid form and are supplied in bottles. The amount of reagent is precisely
dosed to ensure the maximum repeatability.
degree Celsius
degree Fahrenheit
milligrams per liter. mg/L is equivalent to ppm (parts per million)
milliliter
millivolts
°C:
°F:
mg/L:
mL:
mV:
Range 0.0 to 20.0 mg/L
Resolution 0.1 mg/L
Accuracy ±0.5 mg/L ±6% of reading @ 25°C
Typical EMC Deviation ±0.1 mg/L
Light Source Light Emitting Diode
Light Detector Silicon Photocell with narrow band interference filter @ 466nm
Method Adaptation of the mercury(II) thiocyanate method. The chloride ion
displaces thiocyanate ion from mercury(II). The iron(III) present
forms with thiocyanate an orange colored complex. The intensity of
color is proportional to the chloride ion concentration.
Environment 0 to 50°C (32 to 122°F); max 95% RH non-condensing
Battery Type 1 x 9 volt
Auto-Shut off After 10' of non-use in measurement mode ;
after 1 hour of non-use in calibration mode ;
with last reading reminder.
Dimensions 192 x 104 x 69 mm (7.6 x 4.1 x 2.7”)
Weight 260 g (12.7 oz.).
REQUIRED REAGENTS
Code Description Quantity/test
HI 93753A-0 Displacing Reagent 1.0 mL
HI 93753B-0 Complexing Reagent 1.0 mL
PrecisionPrecision
PrecisionPrecision
Precision is how closely repeated measurements
agree with each other. Precision is usually
expressed as standard deviation (SD).
AccuracyAccuracy
AccuracyAccuracy
Accuracy is defined as the nearness of a test
result to the true value.
Although good precision suggests good accuracy,
precise results can be inaccurate. The figure
explains these definitions.
In a laboratory using a standard solution of
10.0 mg/L chloride and a representative lot of
reagent, an operator obtained with a single
instrument a standard deviation of 0.3 mg/L.

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1) GLP/ key
2) CAL CHECK key
3) ZERO/CFM key
4) READ/ /TIMER key
5) ON/OFF key
6) Liquid Crystal Display (LCD)
7) Cuvette alignment indicator
8) Cuvette holder
PRINCIPLE OF OPERATION
FUNCTIONAL DESCRIPTION
Absorption of Light is a typical phenomenon of interaction between electromagnetic radiation and
matter. When a light beam crosses a substance, some of the radiation may be absorbed by
atoms, molecules or crystal lattices.
If pure absorption occurs, the fraction of light absorbed depends both on the optical path length
through the matter and on the physical-chemical characteristics of the substance according to the
Lambert-Beer Law:
-log I/Io = ελ c d
or
A = ελ c d
Where:
-log I/Io=Absorbance (A)
Io=intensity of incident light beam
I=intensity of light beam after absorption
ελ=molar extinction coefficient at wavelength λ
c=molar concentration of the substance
d=optical path through the substance
Therefore, the concentration "c" can be calculated from the absorbance of the substance as the
other factors are known.
Photometric chemical analysis is based on the possibility to develop an absorbing compound
from a specific chemical reaction between sample and reagents. Given that the absorption of a
compound strictly depends on the wavelength of the incident light beam, a narrow spectral
bandwidth should be selected as well as a proper central wavelength to optimize measurements.
The optical system of Hanna's HI 96753 colorimeter is based on special LED and narrow-band
interference filters to guarantee both high performance and reliable results.
HI 96753 block diagram (optical layout)
A microprocessor controlled special LED emits radiation which is first optically conditioned and
beamed to the sample contained in the cuvette. The optical path is fixed by the diameter of the
cuvette. Then the light is spectrally filtered to a narrow spectral bandwidth, to obtain a light
beam of intensity Ioor I.
The photoelectric cell collects the radiation Ithat is not absorbed by the sample and converts
it into an electric current, producing a potential in the mV range.
The microprocessor uses this potential to convert the incoming value into the desired measuring
unit and to display it on the LCD.
The measurement process is carried out in two phases: first the meter is zeroed and then the
actual measurement is performed.
The cuvette has a very important role because it is an optical element and thus requires
particular attention. It is important that both, the measurement and the calibration (zeroing)
cuvettes, are optically identical to provide the same measurement conditions. Whenever possible
use the same cuvette for both. It is necessary that the surface of the cuvette is clean and not
scratched. This to avoid measurement interference due to unwanted reflection and absorption of
light. It is recommended not to touch the cuvette walls with hands.
Furthermore, in order to maintain the same conditions during the zeroing and the measuring
phases, it is necessary to close the cuvette to prevent any contamination.
INSTRUMENT DESCRIPTION

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ERRORS AND WARNINGS
KEYPAD DESCRIPTION
•ON/OFF: to turn the meter on and off.
•ZERO/CFM: to zero the meter prior to measurement, to confirm edited values or to confirm
factory calibration restore.
•READ/ /TIMER: this is a multi-functional key. In measurement mode , press to make a
measurement, or press and hold for three seconds to start a pre-programmed countdown prior
to measurement. In GLP mode press to view the next screen.
•CAL CHECK: this is a bi-functional key. Just press to perform the validation of the meter, or
press and hold for three seconds to enter calibration mode .
•GLP/: this is a bi-functional key. Just press to enter
GLP mode . In calibration mode press
to edit the date and time.
OPERATING MODES
• Measurement mode: default operation mode, enables both validation and measurement.
• Calibration mode: may be entered by keeping CAL CHECK pressed for three seconds (the
“CAL” tag appears), it enables calibration of the instrument.
• GLP mode may be entered by pressing GLP/(“GLP” appears), it enables consulting of
user calibration date or restore factory calibration.
DISPLAY ELEMENTS DESCRIPTION
1) The measuring scheme (lamp, cuvette, detector), appears during different phases of zero or
reading measurement
2) Error messages and warnings
3) The battery icon shows the charging level of the battery
4) The hourglass appears when an internal checking is in progress
5) Status messages
6) The chronometer appears when the reaction timer is running
7) The month, day and date icons appear when a date is displayed
8) Four digit main display
9) Measuring units
10) Four digit secondary display
The instrument shows clear messages when erroneous condition appears. Messages are also displayed
when the obtained values are outside expected range. The beeper is playing a beep on errors.
a) on zero reading
Light High: There is too much light to perform a measurement.
Please check the preparation of the zero cuvette.
Light Low: There is not enough light to perform a measurement.
Please check the preparation of the zero cuvette.
No Light:The instrument cannot adjust the light level. Please
check that the sample does not contain any debris.
Inverted cuvettes: The sample and the zero cuvette are inverted.
Zero: A zero reading was not taken. Follow the instructions of
the measurement procedure for zeroing the meter.
b) on sample reading

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The instructions listed below should be carefully followed during testing to ensure best accuracy.
•Color or suspended matter in large amounts may cause
interference, therefore these should be removed by
treatment with active carbon and by prior filtration.
•For a correct filling of the cuvette: the liquid in the
cuvette forms a concavity on the top; the bottom of this
concavity must be at the same level of the 10 mL mark.
•In order to measure exactly 0.5 mL of reagent with the 1 mL syringe:
(a) push the plunger completely into the syringe and insert the tip into the solution.
(b) pull the plunger up until the lower edge of the seal is exactly on the 0.0 mL mark.
(c) take out the syringe and clean the outside of the syringe tip. Be sure that no drops are
hanging on the tip of the syringe, if so eliminate them. Then, keeping the syringe in
vertical position above the cuvette, push the plunger down into the syringe until the lower
edge of the seal is exactly on the 0.5 mL mark. Now the exact amount of 0.5 mL has
been added to the cuvette, even if the tip still contains some solution.
GENERAL TIPS FOR AN ACCURATE MEASUREMENT
Under Range: A blinking “0.0” indicates that the sample
absorbs less light than the zero reference. Check the procedure
and make sure you use the same cuvette for reference (zero) and
measurement.
Over Range: A flashing value of the maximum concentration
indicates an over range condition. The concentration of the
sample is beyond the programmed range: dilute the sample and
re-run the test.
c) during calibration procedure
Standard Low: The standard reading is less than expected.
Standard High: The standard reading is higher than expected.
Cap error: Appears when external light enters in the analysis
cell. Assure that the cuvette cap is present.
Cooling lamp: The instrument waits for the lamp to cool down.
d) other errors and warnings
Battery low: The battery must be replaced soon.
Dead battery: This indicates that the battery is dead and must
be replaced. Once this indication is displayed, the meter will
lock up. Change the battery and restart the meter.
(c)(a) (b)

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•It is important that the sample does not contain any debris. This would corrupt the reading.
•Each time the cuvette is used, the cap must be tightened to the same degree.
•Whenever the cuvette is placed into the measurement cell, it must be
dry outside, and completely free of fingerprints, oil or dirt. Wipe it
thoroughly with HI 731318 or a lint-free cloth prior to insertion.
•Shaking the cuvette can generate bubbles in the sample, causing
higher readings. To obtain accurate measurements, remove such bubbles
by swirling or by gently tapping the cuvette.
•Do not let the reacted sample stand too long after reagent is added, or accuracy will be lost.
•It is possible to take multiple readings in a row, but it is recommended to take a new zero
reading for each sample and to use the same cuvette for zeroing and measurement.
•After the reading it is important to discard immediately the sample, otherwise the glass
might become permanently stained.
•All the reaction times reported in this manual are referred to 20°C (68°F). As a general rule
of thumb, they should be doubled at 10°C (50°F) and halved at 30°C (86°F).
•In order to maximize accuracy, prior to a measurement follow the validation procedure to
be sure that the instrument is properly calibrated. If necessary, calibrate the instrument.
MEASUREMENT PROCEDURE
STARTUP
Prepare the instrument for measurement as follows:
•Unpack the instrument by removing the dust protection sleeve from the instrument cuvette
holder.
•Place the battery in the instrument as described in the “BATTERY REPLACEMENT” chapter.
•Place the instrument on a flat table.
•Do not place the instrument under direct sun light.
To compensate the meter for the sample turbidity or color, the measurement takes place in two
phases. First, the meter is zeroed using the unreacted sample. After the reagents are added the
reacted sample is measured.
•When the beeper sounds briefly and the LCD
displays dashes, the meter is ready. The blinking
“ZERO” indicates that the instrument needs to
be zeroed first.
•Fill one cuvette (#1) with 10 mL of distilled
water (up to the mark).
•Fill another cuvette (#2) with 10 mL of
sample (up to the mark)
Note: for low chloride ions concentration, rinse
the cuvette a couple of times with sample
before filling it with 10 mL of sample (up to the
mark).
Note: for most accurate results, use two gradu-
ated pipettes to deliver exactly 10 mL of distilled water and 10 mL of sample to the cuvettes.
•Using the 1 mL syringe, add 0.5 mL of HI 93753A-0 Displacing Reagent to each cuvette.
•Replace the caps and mix each cuvette by turning the cuvette upside down for approximately
30 seconds.
•Turn the meter on by pressing ON/OFF.
The display briefly shows all tags on.

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•Remove the caps and use the second 1 mL
syringe to add 0.5 mL of HI 93753B-0
Complexing Reagent to each cuvette.
•Replace the caps and mix each cuvette
by turning the cuvette upside down for
approximately 30 seconds.
•Place the cuvette with the reacted distilled
water (#1) into the cuvette holder and
ensure that the notch on the cap is
positioned securely into the groove.
•Press READ/ /TIMER.The display will
show the countdown prior to zeroing. The
beeper is playing a beep at the end of
countdown period.
Alternatively, wait for 2minutes then just
press ZERO/CFM.
In both cases, the lamp, cuvette and
detector icons will appear on the display,
depending on the zeroing phase.
•After a few seconds, the display will
show “-0.0-”. The meter is now zeroed
and ready for measurement.
•Remove the cuvette.
•Place the other cuvette with the reacted
sample (#2) into the cuvette holder
and ensure that the notch on the cap is
positioned securely into the groove.
# 1
•Press READ/ /TIMER. The lamp, cuvette
and detector icons will appear on the
display, depending on the measurement
phase.
•At the end of measurement, the instrument
directly displays concentration in mg/L of
chloride on the LCD.
INTERFERENCES
The pH of sample after addition of reagents should be about 2. For alkaline samples, neutralize
before adding reagents.
Intensely colored samples will cause interference, therefore they should be adequately treated before
performing the test. Suspended matter in large amounts should be removed by prior filtration.
# 2
VALIDATION PROCEDURE
Use the validation procedure to ensure that the instrument is properly calibrated.
Warning: Do not validate the instrument with any standard solutions other than the HANNA CAL
CHECKTM Standards, otherwise erroneous results will be obtained.
•Turn the meter on by pressing ON/OFF.
•Place the CAL CHECKTM Standard
HI 96753-11 Cuvette A into the cuvette
holder and ensure that the notch on the
cap is positioned securely into the groove.
•When the beeper sounds briefly and the
LCD displays dashes, the meter is ready.

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CALIBRATION PROCEDURE
Note: It is possible to interrupt calibration procedure at any time by pressing CAL CHECK or
ON/OFF keys.
Warning:Do not calibrate the instrument with standard solutions other than the HANNA CAL
CHECKTM Standards, otherwise erroneous results will be obtained.
•Turn the meter on by pressing ON/OFF.
•When the beeper sounds briefly and the
LCD displays dashes, the meter is ready.
•Press and hold CAL CHECK for three
seconds to enter
calibration mode . The
display will show “CAL” during
calibration procedure. The blinking
“ZERO” asks for instrument zeroing.
•Place the CAL CHECKTM Standard
HI 96753-11 Cuvette A into the cuvette
holder and ensure that the notch on the
cap is positioned securely into the groove.
•Press ZERO/CFM and the lamp, cuvette
and detector icons will appear on the
display, depending on the measurement
phase.
•Press ZERO/CFM and the lamp, cuvette
and detector icons will appear on the
display, depending on the measurement
phase.
•After a few seconds, the display will
show “-0.0-”. The meter is now zeroed
and ready for validation.
•Remove the cuvette.
•Place the CAL CHECKTM Standard
HI 96753-11 Cuvette B into the cuvette
holder and ensure that the notch on the
cap is positioned securely into the groove.
•Press CAL CHECK and the lamp, cuvette
and detector icons together with “CAL
CHECK” will appear on the display,
depending on the measurement phase.
•At the end of the measurement the display
will show the validation standard value.
The reading should be within specifications as reported in the CAL CHECKTM Standard
Certificate. If the value is found out of the specifications, please check that the cuvettes are free
of fingerprints, oil or dirt and repeat validation. If results are still found out of specifications,
then recalibrate the instrument.

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•Press GLP/ to edit the desired year
(2000-2099). If the key is kept pressed,
the year number is automatically
increased.
•Press GLP/ to edit the desired day
(01-31). If the key is kept pressed, the
day number is automatically increased.
Note: It is possible to change the editing
from day to year and to month by pressing
READ/ /TIMER.
•When the correct year has been set, press
ZERO/CFM or READ/ /TIMER to
confirm. Now the display will show the
month blinking.
•Press GLP/ to edit the desired month
(01-12). If the key is kept pressed, the
month number is automatically increased.
•When the correct month has been set,
press ZERO/CFM or READ/ /TIMER
to confirm. Now the display will show
the day blinking.
•Press ZERO/CFM to save the calibration
date.
•The instrument displays “Stor” for one
second and the calibration is saved.
DATE INPUT
or
or
•After a few seconds the display will show
“-0.0-”. The meter is now zeroed and ready
for calibration. The blinking “READ” asks for
reading calibration standard.
•Remove the cuvette.
•Place the CAL CHECKTM Standard HI 96753-11
Cuvette B into the cuvette holder and ensure
that the notch on the cap is positioned securely
into the groove.
•Press READ/ /TIMER and the lamp, cuvette
and detector icons will appear on the display,
depending on the measurement phase.
•After measurement the instrument will show for
three seconds the CAL CHECKTM Standard value.
Note: If the display shows “STD HIGH”, the
standard value was too high. If the display
shows “STD LOW”, the standard value was
too low. Verify that both CAL CHECKTM
Standards HI 96753-11 Cuvettes, A and B are
free from fingerprints or dirt and that they are
inserted correctly.
Then the date of the last calibration (e.g.:
”01.08.2008”) appears on the display, or
“01.01.2008” if the factory calibration was
selected before. In both cases the year number
is blinking, ready for date input.