Hanna Instruments HI84529 User manual

INSTRUCTION MANUAL
HI84529
Titratable Acidity
Mini Titrator & pH Meter
for Dairy Products

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the
copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.
Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using this instrument.
This manual will provide you with the necessary information for correct use of this
instrument, as well as a precise idea of its versatility.
If you need additional technical information, do not hesitate to e‑mail us at
[email protected] or view our worldwide contact list at www.hannainst.com.
Dear
Customer,

3
TABLE OF CONTENTS
TABLE OF CONTENTS
1. Preliminary Examination ..................................................................................................... 4
2. Specifications..................................................................................................................... 5
3. General Description & Intended Use ..................................................................................... 7
4. Principle of Operation ......................................................................................................... 9
5. Functional Description....................................................................................................... 10
6. Titrator Startup................................................................................................................. 13
7. Setup Menu ..................................................................................................................... 14
8. Guide to Display Codes...................................................................................................... 19
8.1. Pump Calibration Messages ..................................................................................... 20
8.2. pH Calibration Messages ......................................................................................... 21
8.3. Titration Messages .................................................................................................. 22
9. Electrode Preparation........................................................................................................ 23
10. Electrode Calibration Procedure........................................................................................ 25
11. pH Buffer Temperature Dependence.................................................................................. 29
12. Dosing Pump Installation ................................................................................................ 30
13. Dosing Pump Prime Procedure ......................................................................................... 31
14. Pump Calibration Procedure............................................................................................. 33
15. Titration Procedure.......................................................................................................... 36
15.1. Tips for an Accurate Measurement .......................................................................... 39
15.2. View / Delete Titrator Recorded Data....................................................................... 39
15.3. Titrator GLP Information......................................................................................... 41
16. pH Measurement............................................................................................................ 42
16.1. View / Delete Recorded pH Data............................................................................. 43
16.2. pH Meter GLP Information ..................................................................................... 44
17. PC Interface & Data Transfer............................................................................................ 45
18. Electrode Conditioning & Maintenance.............................................................................. 46
19. Troubleshooting Guide..................................................................................................... 47
20. Accessories..................................................................................................................... 49
Certification........................................................................................................................... 50
Recommendations for Users.................................................................................................... 50
Warranty .............................................................................................................................. 50

4
PrELImINAry ExAmINATION
1. PRELIMINARY EXAMINATION
Remove the instrument and accessories from the packaging and examine it carefully. For further
assistance, please contact your local Hanna Instruments Office or email us at [email protected].
Each HI84529 mini titrator is supplied with:
• FC260B pH electrode
• HI5315 Reference electrode
• HI7662-M Temperature probe
• HI84529-70 Reagent kit for titratable acidity in dairy products
• HI7072 Fill solution,30 mL
• HI700640 Cleaning solution for milk deposits, 20 mL sachet (2 pcs.)
• Capillary dropper pipette (1 pc.)
• 100 mL beaker (2 pcs.)
• Tube set (aspiration tube with titrant bottle cap and dispensing tube with tip)
• Dosing pump valve (1 pc.)
• 5 mL syringe (1 pc.)
• 1 mL syringe with tip (1 pc.)
• Stir bar (1 pc.)
• Power adapter
• Instrument quality certificate
• Instruction manual
Note: Save all packing material until you are sure that the instrument works correctly. Any
damaged or defective item must be returned in its original packing material with the supplied
accessories.

5
SPECIFICATIONS
2. SPECIFICATIONS
Titrator
Range
Low Range %I.a.: 0.01 to 0.20
°SH: 0.4 to 8.9
°D: 1.0 to 20.0
°TH: 1.1 to 22.2
High Range %I.a.: 0.1 to 2.0
°SH: 4.4 to 88.9
°D: 10 to 200
°TH: 11.1 to 222.2
Resolution
%I.a.: 0.01 (Low Range) / 0.1 (High Range)
°SH: 0.1
°D: 0.1 (Low Range) / 1 (High Range)
°TH: 0.1
Accuracy Low Range ± 0.01 %l.a. @ 25 °C
High Range ± 0.1 %l.a. @ 25 °C
Sample size
Low Range 20: 20 mL or 20 g
Low Range 50: 50 mL or 50 g
High Range 20: 20 mL or 20 g
Titration method Acid-base titration
Principle Endpoint titration
Pump speed 10 mL/min
Stirring speed 800 (Low Range) / 1000 (High Range)
Log data Up to 200 samples
pH Meter
Range -2.0 to 16.0 pH / -2.00 to 16.00 pH
Resolution 0.1 pH / 0.01 pH
Accuracy ± 0.01 pH
Calibration 1, 2 or 3 point calibration;
4 available buffers (4.01, 6.00, 8.30, 10.01)
Temperature
compensation Manual or automatic
mV Meter
Range -2000.0 to 2000.0 mV
Resolution 0.1 mV
Accuracy ± 1.0 mV
Log data Up to 200 samples (pH or mV)

6
SPECIFICATIONS
Temperature
Range -20.0 to 120.0 °C (-4.0 to 248.0 °F)
Resolution 0.1 °C
Accuracy ±0.4 °C, without probe error
Electrode FC260B pH electrode
HI5315 Reference electrode
Temperature Probe HI7662-M
Environment 0 to 50 °C (32 to 122 °F); max 95% RH non-condensing
Power Supply 12 Vdc power adapter
Dimensions 235 x 200 x 150 mm (9.2 x 7.9 x 5.9”)
Weight 1.9 kg (67.0 oz.)
Required Reagents
HI84529-50 Titrant Low Range 20
HI84529-51 Titrant High Range 20
HI84529-52 Titrant Low Range 50
HI84529-55 Pump calibration standard

7
GENErAL DESCrIPTION & INTENDED USE
3. GENERAL DESCRIPTION & INTENDED USE
The HI84529 is an affordable, easy to use, microprocessor-based automatic mini titrator and pH
meter designed for the rapid and accurate analysis of Total Titratable Acidity in Dairy Products.
The HI84529 will quickly become a valuable tool by eliminating subjective factors including color
indicators, errors in calculations or erratic titrant additions.
The instrument benefits from Hanna Instruments’ many years of experience as a manufacturer of
quality analytical instrumentation. A clear and well-designed user interface makes the instrument
intuitive and simple to use.
By pressing the Start key in Titrator mode, the instrument will automatically titrate the sample to the
set endpoint, perform all necessary calculations and display the results in the selected unit. At the end
of the titration, another titration can be started by pressing the Restart key.
A dedicated HELP key aids in setup, calibration and troubleshooting.
Other features:
• pH meter / mV meter
• Stir speed control
• Graphic mode to display the titration data
• Data can be stored using the log feature and then exported to a USB stick or transferred to a PC
using the USB connection
• Log on demand for up to 400 samples (200 mV/pH measurements; 200 for titration results)
• GLP feature, to view calibration data for the pH electrode and pump
Significance of Use
Titratable acidity can be expressed in several units; % lactic acid (% l.a.), degree Soxhlet Henkel
(°SH), degree Dornic (°D) or degree Thörner (°TH). Each of these units corresponds to a specific
procedure used to titrate dairy products.
Soxhlet Henkel degrees (°SH)
mostly used in Central Europe exept France and the Netherlands.
This value is a number of mL of 0.25N NaOH used for titration of
100 mL milk, using phenolphtalein as indicator.
Thörner degrees (°Th)
mostly used in Sweden and the CIS. This value is obtained by
titrating 100 mL of milk, diluted with 2 parts of distilled water,
with 0.1N NaOH, using phenolphtalein as indicator
Dornic degrees (°D)
mostly used in Netherlands and France. This value is obtained
by titrating 100 mL of milk, with N/9 (0.11 N) NaOH, using
phenolphtalein as indicator.
Percent lactic acid (%l.a.)
frequently used in the UK, USA, Canada, Australia and New
Zealand This value is obtained in the same way as °D, dividing
the result by 100.

8
GENErAL DESCrIPTION & INTENDED USE
The titratable acidity values will vary depending on the method used. Select Low 50 to titrate a
non-diluted sample, or select Low 20 / High 20 to titrate 20 mL or 20 g samples that are diluted
with twice its volume of deionized a distilled water. The HI84529 uses methods based on AOAC
International and Standard Methods for the Examination of Dairy Products. Both of these methods
report titratable acidity as % lactic acid, a rough conversion factor can be used to convert the results
to the other available units:
From To Divide by
%l.a. °SH 0.0225
%l.a. °D 0.0100
%l.a. °TH 0.0090
The HI84529 can be customized to meet the needs of any dairy analysis lab. Samples can be titrated
by weight or volume, diluted or non-diluted (low range only) and titrated to a fixed pH endpoint that
can be adjusted by the user.

9
PrINCIPLE OF OPErATION
4. PRINCIPLE OF OPERATION
The methodology for titratable acidity in dairy products is based on neutralization reaction where
the acids in the sample (i.e. lactic acid) reacts with a base (i.e. sodium hydroxide) to produce water:
H++OH¯H2O
In an ideal solution, the endpoint of an acid titration corresponds stoichiometrically to the complete
neutralization of the acids present.
This endpoint for this reaction can be determined visually using a color indicator (i.e. phenolphthalein),
however this endpoint becomes very objective in opaque and colored samples. The HI84529 removes
this issue by titrating to a fixed pH endpoint that can be set by the user. The HI84529 can be
customized to meet individual needs with samples titrated by mass or by volume, in two different
ranges, with diluted and non-diluted (low range only). For precise analysis the sample size, volume
of titrant added and titrant concentration must be known.
The HI84529 Titratable Acid in Dairy Products Mini Titrator utilizes a simple sample preparation, a high
quality dosing pump for titrant additions, potentiometric endpoint determination and instantaneous
computations. To maintain the high accuracy of the mini titrator a simple pump calibration is required.
The pump calibration uses a known quantity of a known solution to compensate for changes in the
dosing system, this procedure should be performed regularly.

10
FUNCTIONAL DESCrIPTION
5. FUNCTIONAL DESCRIPTION
Front View
2
3
8
9
7
6
1
5
4
1. Dosing pump valve
2. Aspiration tube
3. Syringe
4. Titrant bottle
5. Dispensing tube
6. Reference electrode
7. Temperature probe
8. pH electrode
9. Electrode holder
Top View
1
4
65
2 3
1. Titrant bottle
2. Dispensing tube
3. Electrode holder
4. Liquid Crystal Display (LCD)
5. Functional keys
6. Keypad

11
FUNCTIONAL DESCrIPTION
Rear View
3
2
1
5
6
7
4
1. Power switch
2. USB connector (PC interface)
3. USB connector (storage interface)
4. Power adapter
5. BNC electrode connector
6. Reference electrode connector
7. Temperature probe connector
Keypad Description
The keypad contains 8 direct keys and 3 functional keys with the following functions:
Press the functional key to select the virtual option displayed above it on the LCD.
Press ESC key to leave the current screen and to return either to the previous screen or to
the main screen. In Setup, exits a parameter without changing the value.
Press ARROW keys to modify the parameters’ values, to scroll the information displayed
while viewing a help screen or to move between the options from the instrument’s Setup
Press CAL key to access the Electrode and Pump calibration options
Press HELP key to access/exit the instrument’s contextual help
Press LOG key to save the current mV/pH reading in pH meter mode and the titration result
Press MENU key to enter Setup, Recall or GLP selection menu, while instrument is in pH
or Titration mode
Press STIR key to start/stop the stirrer
Note: The stirrer starts automatically during pump calibration and titration, it cannot be stopped
by pressing STIR key.

12
FUNCTIONAL DESCrIPTION
Display Indicators
During the instrument’s operation a set of information are displayed on the LCD.
1
2
3
4
5
6
7
8
1. Current time and instrument mode information (pH meter or Titrator)
2. pH electrode condition
3. Instrument status
4. Virtual option keys
5. Stirrer and reading status
6. Main reading information
7. pH temperature compensation mode (Manual or Automatic)
8. Temperature reading
Displayed icons:
Stirrer running (blinks when stirrer is not working properly.)
Pump running
Unstable reading
Parameter can be modified
Dosing Pump
The dosing pump is based on a valve that automatically moves the titrant between the titrant
bottle and syringe when filling the syringe and between the syringe and sample when dispensing.
A replaceable 5 mL plastic syringe is used to limit the amount of titrant used per test to ensure the
highest possible accuracy. Before a set of titrations, it is necessary to prime the dosing system.
Note: Once titrations have been completed, the dosing system should be cleaned with deionized
water using the prime feature.

13
TITrATOr STArTUP
6. TITRATOR STARTUP
This is a general outline of the steps required to perform a titration. The following topics are expanded
upon in each section that follows.
• Place the instrument on a flat table. Do not place the instrument in direct sun light.
• Connect the power adapter to the instrument.
• Turn the instrument on using the power switch on the rear panel of the instrument.
• Set up the instrument. See SETUP MENU section for details.
• Connect the pH electrode to the instrument.
• Connect the reference electrode to the instrument.
• Connect the temperature probe to the instrument.
• Calibrate the pH electrode.
• Connect the tubes and the valve. See DOSING PUMP INSTALLATION section for the procedure.
• Remove the titrant bottle cap and replace it with the bottle cap with tubes. Place the titrant bottle
in the appropriate place on the titrator top.
Note: Different titrants are required based on the concentration. See PUMP CALIBRATION
PROCEDURE for details.
• Prime the syringe. To assure high accuracy, verify there are no air bubbles in the syringe or tubing.
• Calibrate the pump.
Note: Different volumes of standards are required based on the concentration. See PUMP
CALIBRATION PROCEDURE for details.
• Prepare the sample.
• Run a titration and log sample results.

14
SETUP mENU
7. SETUP MENU
The titrator’s setup menu may be accessed from the main screen (meter or titrator) by pressing the
MENU key, then Setup.
A list of setup parameters will be displayed with currently configured setting.
While in the Setup menu, it is possible to modify the instrument’s operation parameters. The ARROW
keys permit the user to scroll the setup parameters.
Press HELP to view the contextual help.
Press ESC to return to the main screen.
Concentration Unit
% l.a., °SH, °D or °TH
Press the corresponding virtual option key to change the option.
% l.a. - % lactic acid
°SH - °Soxhlet Henkel
°D - °Dornic
°TH - °Thörner
Acidity Titration
Low 20, Low 50 or High 20
Press the corresponding virtual option key to select the desired range.
Note: Different titrants are required for each range.
Sample Type
Mass or Volume
Samples can be added by volume or by mass. For improved accuracy all samples should be added
by mass.

15
SETUP mENU
Calibration Timeout
Disabled or 1 to 7 days
This option is used to set the number of days before the pH calibration expired warning message is
displayed.
Press Modify to access the calibration timeout screen.
Use the ARROW keys to select the value.
Press Select to confirm or ESC to return to the setup menu without saving the changes.
pH Resolution
0.1 or 0.01
Press the displayed virtual option key to change the option.
Temperature Unit
°C, °F or K
Press the virtual option key to change the option.
Time
Press the Modify key to change the time and time format.
Press Format to switch between 12 hour (am/pm) and 24 hour mode.
Press to highlight the value to be modified. Use the ARROW keys to change the value. Press
Accept to confirm the new value or ESC to return to the setup.

16
SETUP mENU
Date
Press the Modify key to change the date and date format.
Press Format to cycle between the available date formats.
Press to highlight the value to be modified. Use the ARROW keys to change the value. Press
Accept to confirm the new value or ESC to return to the setup.
Key Beep
Select Enable to activate or Disable to deactivate the Key Beep function.
If enabled, a short beep will be heard every time a key is pressed.
Error Beep
Select Enable to activate or Disable to deactivate the Error Beep function.
If enabled, a beep will be heard when an error condition occurs.
Decimal Separator
This option allows the user to select the symbol used for a decimal separator.
LCD Contrast
This option is used to set the display’s contrast.
Press Modify to change the display’s contrast.
The default value is 8.

17
SETUP mENU
Use the ARROW keys or / to increase/decrease the value.
Press Accept to confirm the value or ESC to return to the setup menu.
LCD Backlight
Press Modify to change the backlight value.
The default value is 3.
Use the ARROW keys or / to increase/decrease the backlight level.
Press Accept to confirm or ESC to return to the setup menu.
pH Endpoint
Press Modify to change the pH endpoint value.
The default value is 8.3 pH.
Use the ARROW keys to select the value.
Press Accept to confirm or ESC to return to the setup menu without saving the changes.
Language
The only available language is English.

18
SETUP mENU
Tutorial
Enable or Disable the Tutorial. This helpful tool offers additional information during calibration and
titration.
Meter Information
Press Select to view the firmware version, language version, mV factory calibration date and time and
temperature factory calibration date and time.
Press ESC to return to the setup menu.
Restore Factory Settings
Press Select to restore the factory settings.
Press Yes to confirm the restore process or No to return without restoring.
Press ESC to return to the setup menu.

19
GUIDE TO DISPLAy CODES
8. GUIDE TO DISPLAY CODES
This screen appears when the instrument is turned on during
the initialization process.
Titration screen display.
Titration screen when a titration is in progress.
Prime burette screen.
Prime burette screen when the dosing system is running.
This error message appears when the pump is not working
properly. Check the tubing, valve and syringe. Press Restart
to try again.
This screen appears when the titrator is in calibration mode.
Press Pump to calibrate Pump.
Press Electrode to calibrate pH electrode.

20
GUIDE TO DISPLAy CODES
8.1. PUMP CALIBRATION MESSAGES
Pump calibration is initiated by pressing the Start key.
This screen appears while pump calibration is in progress.
Press ESC or Stop key to return to the Pump Calibration
screen.
This error message appears during pump calibration when
the endpoint can not be reached and the maximum amount
of titrant is exceeded. Check standard, electrode and/or
dosing system and try again.
The calibration was outside the acceptable limits. Prepare a
new standard and try again.
This error message appears when the pH reading exceeds the
acceptable input limits (-2.00 < pH > 16.00).
This screen appears when the stirrer is not working properly.
Check the stir bar and beaker content. Press Restart to try
again.
This error message appears when the pump is not working
properly. Check the tubing, valve and syringe. Press Restart
to try again.