Hanna Instruments HI96816 User manual

HI96816
Refractometer for Sucrose
in Wine and Grape Products
Measurements
INSTRUCTION MANUAL

Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using
this instrument.
This manual will provide you with the necessary informa‑
tion for correct use of this instrument, as well as a precise
idea of its versatility.
If you need additional technical information, do not
hesitate to e‑mail us at [email protected] or view our
worldwide contact list at www.hannainst.com.
Dear
Customer,
All rights are reserved. Reproduction in whole or in part is prohibited
without the written consent of the copyright owner, Hanna Instruments
Inc., Woonsocket, Rhode Island, 02895, USA.

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Preliminary Examination ........................................................
General Description................................................................
Specifications........................................................................
Principle of Operation............................................................
Functional Description............................................................
Display Elements...................................................................
Measurement Guidelines ........................................................
Calibration Procedure.............................................................
Measurement Procedure.........................................................
Making a Standard % Brix Solution .......................................
To Change Temperature Unit ..................................................
To see % Brix Value ..............................................................
Battery Replacement..............................................................
Error Messages .....................................................................
Accessories ...........................................................................
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TABLE OF CONTENTS
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Remove the instrument from the packing materials and examine carefully
to ensure no damage has occurred during shipping. If any damage has
occurred, contact your local Hanna Instruments Office.
Each HI96816 instrument is supplied with:
• 9 V battery
• Instruction manual
• Plastic pipette
• Quality Certificate
Note: Save all packing material until you are sure that the instrument
functions correctly. A defective instrument must be returned in its
original packing.
PRELIMINARY EXAMINATION

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The HI96816 Digital Wine Refractometer is rugged portable, water resistant
devices that benefit from Hanna Instruments’ years of experience as a
manufacturer of analytical instruments. Hanna Instruments offers five related
wine refractometers to fill the differing requirements found in the industry.
All are equally valid ways to measure the sugar content of grape or must
samples in the field or winery.
The HI96816 is optical instruments that are based on the measurement of the
refractive index of a solution. The measurement of refractive index is simple
and quick and provides the vintner a standard accepted method for sugar
content analysis. Samples are measured after a simple user calibration with
deionized or distilled water. Within seconds the instrument measures the
refractive index of the grape.
These digital refractometers eliminate the uncertainty associated with
mechanical refractometers and are easily portable for measurements in
the field. Temperature (in °C or °F) is displayed simultaneously with the
measurement on the large dual level display along with icons for Low Power
and other helpful message codes.
Key features include:
• Dual‑level LCD
• Automatic Temperature Compensation (ATC)
• Easy setup and storage
• Battery operation with Low Power indicator (BEPS)
• Automatically turns off after 3 minutes of non‑use
• Single‑point calibration with distilled or deionized water
• Waterproof models offers IP65 waterproof protection
• Quick, precise results readings are displayed in approximately 1.5 seconds
• Small sample size as small as 2 metric drops.
GENERAL DESCRIPTION

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Range
4.9 to 56.8 %V/V Potential Alcohol
(10.0 to 75.0 %Brix) /
0.0 to 80.0 °C (32.0 to 176.0 °F)
Resolution 0.1 %V/V / 0.1 °C (0.1 °F)
Accuracy ± 0.2 %V/V / ± 0.3 °C (±0.5 °F)
Temperature
Compensation
Automatic between 10.0 and 40.0 °C
(50.0 ‑ 104.0 °F)
Measurement Time Approximately 1.5 seconds
Minimum Sample
Volume 100 µL (cover prism totally)
Light Source Yellow LED
Sample Cell Stainless Steel ring and flint glass prism
Case Material ABS
Enclosure Rating IP65
Battery Type / Life 9V / 5000 readings
Auto‑Off After 3 minutes of non‑use
Dimensions 192 x 102 x 69mm (7.6 x 4.1 x 2.7”)
Mass 350 g (12.3 oz.)
SPECIFICATIONS

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The %Brix determination is made by measuring the refractive index of a
solution. Refractive Index is an optical characteristic of a substance and the
number of dissolved particles in it. Refractive Index is defined as the ratio of
the speed of light in empty space to the speed of light in the substance. A
result of this property is that light will “bend”, or change direction, when it
travelsthroughasubstanceofdifferentrefractiveindex.Thisiscalledrefraction.
When passing from a material with a higher to lower refractive index, there
is a critical angle at which an incoming beam of light can no longer refract,
but will instead be reflected off the interface. The critical angle can be used
to easily calculate the refractive index according to the equation:
sin (θcritical) = n2/ n1
Wheren2istherefractiveindexofthelower‑densitymedium;n1istherefractive
index of the higher‑density medium.
In the HI96816, light from an LED passes through a prism in contact with
the sample. An image sensor determines the critical angle at which the light
is no longer refracted through the sample.
PRINCIPLE OF OPERATION

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8. Battery Cover
9. Battery Compartment
TOP VIEW
BOTTOM
1. Liquid Crystal Display (LCD)
2. Secondary Display
3. Primary Display
4. READ Key (User Measurement)
5. ZERO Key (User Calibration)
6. ON/OFF
7. Stainless Steel Sample Well and Prism
The HI96816 potential alcohol curve is based on the tables found in the
European Economic Community Commission Regulation No 2676/90 of
17 September 1990, Determining Community Methods for the Analysis of
Wine and International Organization of Vine and Wine (OIV). The potential
alcohol curve is based on the following equation:
Potential alcohol %V/V = g/L of Sugar / 16.83
FUNCTIONAL DESCRIPTION

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1. Battery (blinks when low battery condition detected)
2. Primary Display (displays measurement and error messages)
3. Measurement in Progress Tag
4. SETUP: Factory Calibration Tag
5. CAL: Calibration Tag
6. Measurement Unit
7. Automatic Temperature Compensation
(blinks when temperature exceeds 10.0‑40.0 °C / 50.0‑104.0 °F range)
8. Temperature Units
9. Secondary Display (displays temperature measurements; when blinking,
temperature has exceeded operation range: 0.0‑80.0 °C / 32.0‑176.0 °F)
DISPLAY ELEMENTS

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• Handle instrument carefully. Do not drop.
• Do not immerse instrument under water.
• Do not spray water to any part of instrument except the “sample well”
located over the prism.
• The instrument is intended to measure Wine and Grape products. Do not
expose instrument or prism to solvents that will damage it. This includes
most organic solvents and extremely hot or cold solutions.
• Particulate matter in a sample may scratch the prism. Absorb sample
with a soft tissue and rinse sample well with deionized or distilled water
between samples.
• Use plastic pipettes to transfer all solutions. Do not use metallic tools
such as needles, spoons or tweezers as these will scratch the prism.
• To reduce the effects of evaporation or absorption of water when taking
readings over a period of time, the prism and sample well can be covered
with plastic wrap.
MEASUREMENT GUIDELINES