Hanna Instruments HI98198 OPDO User manual

INSTRUCTION MANUAL
HI98198
opdo®
Optical Dissolved Oxygen Meter

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the
copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.
Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using this meter.
This manual will provide you with the necessary information for correct use of
this meter, as well as a precise idea of its versatility.
If you need additional technical information, do not hesitate to e‑mail us at
[email protected]orviewourworldwidecontactlistatwww.hannainst.com.
Dear Customer,

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TABLE OF CONTENTS
TABLE OF CONTENTS
1. PRELIMINARY EXAMINATION ............................................................................................... 4
2. GENERAL DESCRIPTION....................................................................................................... 5
3. FUNCTIONAL DESCRIPTION.................................................................................................. 6
4. SPECIFICATIONS................................................................................................................. 7
5. THEORY OF OPERATION ...................................................................................................... 9
6. PROBE COMPONENTS....................................................................................................... 10
7. INITIAL PREPARATION....................................................................................................... 11
8. SETUP............................................................................................................................. 13
8.1. PARAMETER SCREENS ............................................................................................. 15
9. CALIBRATION ................................................................................................................... 26
9.1. DO CALIBRATION .................................................................................................... 26
9.2. PRESSURE CALIBRATION ......................................................................................... 30
9.3. TEMPERATURE CALIBRATION ................................................................................... 31
10. GOOD LABORATORY PRACTICE (GLP)................................................................................. 33
11. MEASUREMENT.............................................................................................................. 34
12. LOGGING........................................................................................................................ 35
12.1. LOGGING THE CURRENT DATA ................................................................................ 35
12.2. VIEW LOGGED DATA .............................................................................................. 35
13. RETRIEVING DATA........................................................................................................... 37
13.1. METER TO PC........................................................................................................ 37
13.2. METER TO USB DRIVE........................................................................................... 37
14. DO MEASUREMENT......................................................................................................... 39
15. BOD MEASUREMENT ...................................................................................................... 40
16. OUR MEASUREMENT ...................................................................................................... 45
17. BATTERY REPLACEMENT .................................................................................................. 50
18. PROBE MAINTENANCE .................................................................................................... 51
19. TROUBLESHOOTING GUIDE ............................................................................................. 54
20. ACCESSORIES................................................................................................................. 55
CERTIFICATION...................................................................................................................... 56
RECOMMENDATIONS FOR USERS............................................................................................ 56
WARRANTY ........................................................................................................................... 57

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PRELIMINARY EXAMINATION
1. PRELIMINARY EXAMINATION
Remove the instrument and accessories from the packaging and examine it carefully. For further
assistance, please contact your local Hanna Instruments Office or email us at [email protected].
Each HI98198 opdo®is supplied in a custom thermoformed durable carrying case with:
• HI764113 Optical DO probe with built-in temperature sensor, protective shield and 4 m (13’4) cable
• HI764113-1 Smart Cap with o-ring
• HI7040 Bicomponent Zero Oxygen Solution
• Calibration/storage vessel
• 100 mL plastic beaker (2 pcs.)
• 6 g sachet with silicone grease
• Syringe
• Lens wipe (1 pc.)
• 1.5V AA batteries (4 pcs.)
• Instrument quality certificate
• Probe quality certificate
• DO Smart Cap quality certificate
• USB Type A to C cable
• Instruction manual
Note: Save all packing material until you are sure that the instrument works correctly. Any
damaged or defective item must be returned in its original packing material with the supplied
accessories.

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GENERAL DESCRIPTION
2. GENERAL DESCRIPTION
The HI98198 opdo®meter is a rugged, portable dedicated dissolved oxygen (DO) meter designed for
fresh and saltwater measurements of dissolved oxygen. This professional, waterproof meter complies
with IP67 standards and measures DO, barometric pressure, and temperature.
The HI98198 opdo meter is only compatible with HI764113 digital dissolved oxygen probe.
Concentration measurements are automatically compensated for barometric pressure, temperature
and salinity. Barometric pressure and temperature are automatically measured and compensated.
Salinity is automatically compensated by setting manually the salinity concentration of the water
being measured. The meter also has a built in application to measure and calculate BOD (Biological
Oxygen Demand), OUR (Oxygen uptake rate), and SOUR (Specific Oxygen Update Rate).
Features
• Text messages on the graphic LCD display to warn and guide the user.
• Back lighted display.
• One or two points calibration at 0 or/and 100% saturation (with auto recognition).
• A single point manual calibration in mg/L or % saturation using a reference method for
calibration value.
• A dedicated HELP key for assistance anytime.
• A user selectable “Calibration due” warning.
• A dedicated GLP key that includes at last 5 calibrations with time, date, calibration points as
well as barometric pressure, temperature and salinity setting.
• AutoEnd freezes the next stable measurement value on the display.
• Log on demand with 4000 records capability.
• USB-C port for easy data transfer to memory stick, PC or other compatible device.
Note: HI98198 opdo complies with the limits for a Class B digital device, pursuant to part
15 of the FCC Rules. It provides reasonable protection against harmful interference in a
residential installation; and generates, uses and can radi-ate radio frequency energy. If not used
in accordance with the instructions, may cause harmful interference to radio communications.
However, there is no guarantee that interference will not occur in a particular installation. If this
equipment does cause harmful interference, which can be determined by switching the meter off
and on, the user is encouraged to try to correct the interference by one or more of the following
measures:
• Reposition the meter.
• Increase the separation between meter and receiver.

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FUNCTIONAL DESCRIPTION
3. FUNCTIONAL DESCRIPTION
Front View Top View
1. Liquid Crystal Display (LCD)
2. F1, F2, F3 functional keys
3. / keys to manually increase/decrease the parameters or to scroll through the menu
4. ON/OFF ( ) key, to turn the meter ON and OFF
5. LIGHT ( ) key, to toggle display back-lighting
6. GLP key, to display Good Laboratory Practice information
7. CAL key, to enter/exit calibration menu
8. SETUP key, to enter/exit Setup menu
9. RCL key, to enter/exit view logged data mode (RCL means RECALL)
10. MODE key to switch between DO, BOD, OUR and SOUR applications
11. RANGE key, to change between % saturation or concentration in DO measurement mode
12. HELP key to enter/exit contextual help
13. ESC key to leave current mode, exit calibration, setup, help, etc.
14. Quick connect DIN connector
15. USB-C connector
16. Junction for barometric pressure sensor

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SPECIFICATIONS
4. SPECIFICATIONS
HI98198 Meter with HI764113 Probe
Dissolved Oxygen Range 0.00 to 50.00 mg/L / 0.0 to 500.0% saturation
Resolution 0.01 mg/L / 0.1% saturation
Accuracy 1.5% of reading ± 0.01mg/L for 0.00-20.00mg/L
5% of reading for 20.00-50.00mg/L
1.5% of reading ±0.1% for 0.0-200.0%
5% of reading for 200.0-500.0%
Barometric Pressure Range 420 to 850 mmHg
Resolution 1 mmHg
Accuracy ±3 mmHg within ±15% from the calibration point
Temperature Range -5.0 to 50.0 °C (23 to 122 °F)
Resolution 0.1 °C (0.1 °F)
Accuracy ±0.3 °C (±0.4 °F)
DO Calibration One or two points automatic calibration at 100% (8.26 mg/L) and 0% (0 mg/L).
Single point manual using a value entered by the user in % saturation or mg/L.
Temperature Calibration Single point anywhere within temperature range
Pressure Calibration Single point anywhere within pressure range
Temperature Compensation Automatic from -5.0 to 50.0 °C (23.0 to 122.0 °F)
Pressure Compensation Automatic from 420 to 850 mmHg
Salinity Compensation Automatic from 0 to 70 PSU (manually set)
DO Probe HI764113 Optical Probe
Log On demand with 4000 records capability
Battery Type / Life 1.5V AA batteries (4 pcs.) / approx. 200 hours of continuous use without
backlight (50 hours with backlight)
Auto Power Off User selectable: 5, 10, 30, 60 minutes or disabled
PC Connectivity USB-C
Dimensions 185 x 93 x 35.2 mm (7.3 x 3.6 x 1.4”)
Meter Weight
(with batteries) 450 g (15.9 oz)
Case Ingress Protection
Rating IP67
Environment 0 to 50 °C (32 to 122 °F) max. RH 100%

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SPECIFICATIONS
HI764113 Probe
Probe body material ABS
Smart Cap material Polypropylene
Cable jacket material PVC
Cable length 4 m (13.1 ft.), 10 m (32.8 ft.), and 20 m (65.6 ft.) options
Probe guard 316 Stainless Steel
Temperature Measurement Thermistor
Pressure 20 m (29 PSI)
Probe Dimensions
(with Guard) 174 X 25 mm (6.8 X 1”)
Response Time (t95) 45 seconds
Probe Weight
(with Guard)
400 g (14.2 oz)
4 m (13.1 ft.) cable length
Probe Ingress Protection Rating IP68
Sensor type Optical; Luminescence Quenching
Origin Assembled in USA

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THEORY OF OPERATION
5. THEORY OF OPERATION
The Hanna HI764113 optical DO sensing probe is based on the principle of fluorescence quenching.
The sensing method features an immobilized Pt based luminophore that is excited by the light of a
blue LED and emits a red light. Dissolved oxygen quenches this excitation. When there is no oxygen
present, the lifetime of the signal is the greatest; as oxygen hits the sensing surface, the lifetime
becomes shorter. The intensity and lifetime are inversely proportional to the amount of oxygen present;
as oxygen interacts with the luminophore it reduces the intensity and lifetime of the luminescence. The
lifetime of the luminescence is measured by a photodetector, and is used to calculate the dissolved
oxygen concentration. This is in turn reported by the meter as a % saturation or mg/L reading of
Dissolved Oxygen.
The major components of the probe include a blue LED for excitation, a red LED that is used as a
reference light, and a photodetector. The Smart Cap is locked in place on the optical probe and
includes the immobilized O2sensitive luminophore with rugged insoluble black oxygen permeable
protective layer.
Over time, the sensor’s optical components can age but are compensated for by using the reference
signal to compensate the measuring path. As a result, the sensor provides accurate DO measurements
over long periods of time without the need for frequent calibration.

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PROBE COMPONENTS
6. PROBE COMPONENTS
1. Strain relief
2. ABS Probe body
3. Temperature Sensor
4. O-ring Seal
5. Optical window
6. Alignment key
7. Smart Cap
8. RFID Tag
9. Embedded O2sensitive luminophore with black protective layer
10. Protective shield

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INITIAL PREPARATION
7. INITIAL PREPARATION
Install the supplied batteries into the meter. See BATTERY REPLACEMENT for details.
To prepare the meter for field measurements close the USB communication socket with the attached
stopper.
Turn the meter ON by pressing ON/OFF key.
At start-up the meter will show the Hanna Instruments logo for a few seconds, followed by the
percentage indication of the remaining battery life.
Before connecting the probe for the first time, press SETUP and using / arrows navigate to
Date/Time. Press Modify and set the current date and time.
Note: Verify time and date are properly set on meter prior to probe initialization.
1. Remove the HI764113 from the carrying case. Remove stainless
steel guard from probe body if it was installed. Use care not to
leave fingerprints on the optical window.
2. Remove the syringe plunger; cut top off supplied sachet with
silicone grease and empty contents into the syringe. Using the
syringe, sparingly lubricate the o-ring with a thin film of the
supplied grease. Avoid getting any kind of grease or fingerprints
onto the optical window.
3. Remove the HI764113-1 optical cap from the container. Align
the notched cutout arrow on the Smart Cap with the matching
guide on the probe body.
4. Slide and press the Smart Cap onto the HI764113 body
until the cap snaps in place. Once the cap is installed,
it should not be removed unless a new cap is required.
5. Connect the HI764113 probe to the HI98198 meter
by plugging the DIN connector to the socket located on
the top of the meter.
6. Power the meter to initiate the cap timer.
Note: Turn off the meter before connecting or
disconnecting the probe.

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INITIAL PREPARATION
A probe info screen will be displayed momentarily before defaulting to the measurement screen:
“No Probe” message is displayed on meter if the probe connector is not plugged in properly.
The “No cap info detected. Install the cap and press Continue.“ message on the meter indicates that
the Smart Cap is missing or not properly installed on the probe.
No conditioning period is required when using a HI764113 DO probe.
Store the HI764113 probe in the HI98198 carrying case when not in use.
For frequent use and short term storage it is recommended to remove the stainless steel guard and
replace with the storage vessel that has a small amount of deionized water. The probe can also be
stored with the stainless steel guard on in a beaker containing deionized water.
For longer term storage, remove the stainless steel guard and replace with the storage vessel.

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SETUP
8. SETUP
Setup menu allows viewing and modifying the measurement parameters.
The following table lists the Setup parameters, their valid range and the factory default settings.
Item Description Valid value Default
Log Separator Log file column separator Comma, Semicolon Comma
Salinity The solution’s salt content 0 to 70 PSU 0 PSU
Manual Pressure Used to manually set the pressure
and disable the automatic
barometer measurement
Enabled or disabled Disabled
Pressure Unit mmHg, inHg, atm,
mbar, psi, kPa
mmHg
Temperature Unit °C or °F °C
Calibration Timeout Calibration due timer Disabled, 10 to
70 days
Disabled
BOD Configuration
Sample min delta DO The minimum diff. between the
start and the end DO val.
0.00 to 50.00 mg/L 0.00 mg/L
Sample min end DO The minimum end DO value 0.00 to 50.00 mg/L 0.00 mg/L
Seed min delta DO The minimum diff. between the
start and the end DO val.
0.00 to 50.00 mg/L 0.00 mg/L
Seed min end DO The minimum end DO value 0.00 to 50.00 mg/L 0.00 mg/L
OUR Configuration
Min time The minimum time for the OUR test 1 to 3600 s 1 s
Max time The maximum time for the OUR test 1 to 3600 s 3600 s
Min start DO The minimum DO value for starting
the OUR test
0.01 to 50.00 mg/L 0.01 mg/L
Min end DO The minimum DO value at the end
of the OUR test
0.00 to 50.00 mg/L 0.00 mg/L
Total volume The total volume of the solution to
be tested
0.1 to 300.0 mL 0.1 mL
Sample volume The volume of sample in the
solution to be tested
0.1 to 300.0 mL 0.1 mL

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SETUP
SOUR Configuration
Min time The minimum time for the SOUR test 1 to 3600s 1 s
Max time The maximum time for the SOUR test 1 to 3600s 3600 s
Min start DO The minimum DO value for starting
the SOUR test
0.01 to 50.00 mg/L 0.01 mg/L
Min end DO The minimum DO value at the end
of the test
0.00 to 50.00 mg/L 0.00 mg/L
Total volume The total volume of the solution to
be tested
0.1 to 300.0 mL 0.1 mL
Sample volume The volume of sample in the solution
to be tested
0.1 to 300.0 mL 0.1 mL
Solids weight Total solids or Volatile Suspended
solids weight
0.1 to 300.0 g/L 0.1 g/L
SOUR @ 20 °C Correct the SOUR value to 20 °C Enabled or Disabled Disabled
Autodelete BOD start data Automatically delete BOD start data,
after BOD compute
Enabled or Disabled Disabled
Backlight Backlight Level 1 to 7 4
Contrast Contrast Level 0 to 20 10
Auto Light off Time backlight remains ON 1, 5, 10, 30 minutes 1
Auto Power off Time until meter is powered OFF Disabled or 5, 10, 30,
60 minutes
30
Date / Time 01.01.2006 to
12.31.2099
00 :00 to 23 :59
current date/
time
Time Format AM/PM or 24 hours 24 hours
Date Format DD / MM / YYYY
MM / DD / YYYY
YYYY / MM / DD
YYYY / MM / DD
YYYY - MM - DD
Mon DD, YYYY
DD - Mon - YYYY
YYYY-Mon-DD
YYYY/MM/DD
Language Message display language Up to 3 languages English
Beep ON Beeper Status Enabled or Disabled Disabled
Instrument ID Meter identification 0000 to 9999 0000
Meter Info Displays general information
Probe Info Displays probe and cap information

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SETUP
8.1. PARAMETER SCREENS
Log Separator
Option: Comma (,) or Semicolon (;)
Log separator or CSV file separator is a special character used to separate columns in the CSV log file.
The field separator depends upon regional preferences.
Press the function key to modify the CSV file separator.
Salinity
Option: 0 to 70 PSU
Dissolved oxygen solubility decreases if water contains salts. This parameter is used to compensate
concentration measurements (mg/L) made in brackish or sea water samples. The salinity is expressed
in PSU scale and is manually entered by the user. The DO concentration will be automatically
compensated to improve the accuracy during calibration and measurement. Enter the salinity value to
be close to the known salt level of the sample. Seawater typically has a salinity of 35 and the oxygen
solubility is 18% less as compared to fresh water at 25 °C. By entering the approximate salinity value,
the calibration and subsequent concentration measurement will be compensated to display the correct
oxygen concentration. An 18% error would result if the salinity value is not entered.
Highlight Salinity.
Press Modify.
Use / keys to change the salinity value. Press Accept to confirm or ESC to exit without saving.
Manual Pressure
Option: Enable or Disable
The HI98198 has a built in barometer for automatic compensation of ambient pressure for oxygen
measurement. The user may enable Manual Pressure to disregard the barometer measurement and

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SETUP
manually enter a pressure value which will be used for oxygen measurements. Once enabled, pressure
values are entered from the measurement screen using the / keys.
Highlight Manual pressure.
Press the displayed functional key to enable or disable the feature.
Pressure Unit
Option: mmHg, inHg, atm, mbar, psi, kPa
The HI98198 has the ability to convert and display pressure measurements in user selected units.
Automatic and Manual Pressure will utilize these units.
To change the pressure measurement unit highlight Pressure unit and press Modify.
Using / keys highlight the desired pressure measurement unit.
Press Accept to confirm or ESC to exit without saving.
Temperature Unit
Option: ˚C or ˚F
The HI98198 has the ability to convert and display temperature measurements in degree Celsius or
Fahrenheit.
Highlight Temperature Unit.
To change the temperature unit, press the displayed functional key.

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SETUP
Calibration Timeout
Option: Disabled, 10, 20, 30,40,50, 60 or 70 days
The HI764113 dissolved oxygen probe utilizes optical technology which offers significant advantages
over polarographic or galvanic type dissolved oxygen probes. Some advantages include reduced
maintenance, simplified handling and infrequent calibrations. However if a user wishes to validate or
calibrate on a SOP schedule, a timer may be set as a reminder.
The Calibration time out parameter can be set from 10 to 70 days or can be disabled.
The meter is provided with a real time clock (RTC) and is used to monitor the time elapsed since the
last DO calibration.
The calibration timer is reset every time the meter is calibrated and the “CAL DUE” status is triggered
when the meter detects a calibration time out. The “CAL DUE” tags will start blinking to warn the user
that the meter should be recalibrated.
If the calibration timeout is changed (e.g. to 20 days), then the timer will be immediately reset.
Notes:
Before the DO calibration is cleared (default values loaded) the display always shows the
“CAL DUE”.
Before an abnormal condition in the RTC is detected, the meter forces the “CAL DUE” status.
After a user temperature or pressure calibration is made (or cleared), the “CAL DUE” message
will be activated.
Press SETUP key.
Highlight Calibration timeout.
Press Modify.
Use the / keys to set the desired value.
Press Accept to confirm or ESC to return without saving.
Note: If enabled, a ”CAL DUE” warning will be shown on the display after the Calibration timeout
period has expired.

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SETUP
BOD Configuration
When making BOD measurements, the BOD method configuration parameters must be filled in. These
parameters will be used to flag error limits for the determination. Ignore this parameter if not making
BOD measurements.
Highlight BOD configuration.
Press Select.
Press / keys to modify the selected parameter’s value.
Press Prev/Next to select a different parameter.
Press Save to save the new BOD configuration.
Press ESC to leave without changing.
Parameters:
• Sample min ∆ DO - the minimum acceptable difference between the initial and final DO
values for a sample. If the difference is less than this value the meter will show a warning
message when evaluating the BOD.
Range: 0.00 to 50.00 mg/L.
• Sample min end DO - the minimum acceptable final DO value for a sample. If the final DO
value is less than this value the meter will show a warning message when evaluating the BOD.
Range: 0.00 to 50.00 mg/L.
• Seed min ∆ DO - the minimum acceptable difference between the initial and final DO values
for a seed sample. If the difference is less than this value the meter will show a warning message
when evaluating the BOD.
Range: 0.00 to 50.00 mg/L.
• Seed min end DO - the minimum acceptable final DO value is less than this value the meter
will show a warning message when evaluating the BOD.
Range: 0.00 to 50.00 mg/L.
OUR Configuration
The OUR method configuration allows the user to set the parameters related to the OUR measurement.
OUR is used to determine the oxygen consumption or respiration rate. It is defined as the mg/L of
oxygen consumed per hour.
These parameters will be used to flag error limits and make dilution calculations for the determination.
Ignore this parameter if not making OUR measurements.

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SETUP
Highlight OUR configuration and press Select.
Press / keys to modify the selected parameter’s value.
Press Prev/Next to select a different parameter.
Press Save to save the new OUR configuration.
Press ESC to leave without changing.
Parameters:
• Min time - the minimum time for the OUR test.
Range: 1 to 3600 seconds.
• Max time - the maximum time for the OUR test. The test will stop automatically when the
maximum time has elapsed.
Range: 1 to 3600 seconds.
• Min start DO - the minimum accepted DO value for starting the OUR test. If the DO reading is
less than this value the test cannot be started.
Range: 0.01 to 50.00 mg/L.
• Min end DO - the minimum accepted DO value at the end of the test. If the DO reading at the
end of the OUR test is less than this value a warning message will be displayed.
Range: 0.00 to 50.00 mg/L.
• Total volume - the volume of the diluted mixture.
Range: 0.1 to 300.0 mL
• Sample volume - the volume of sample in the diluted mixture.
Range: 0.1 to 300.0 mL.
SOUR Configuration
Specific Oxygen Uptake Rate (SOUR), also known as the oxygen consumption or respiration rate, is
defined as the milligram of oxygen consumed per gram of volatile suspended solids (VSS) per hour.
These parameters will be used to flag error limits and make dilution calculations for the determination.
Ignore this parameter if not making SOUR measurements.

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SETUP
Highlight SOUR configuration and press Select.
Press / keys to modify the selected parameter’s value.
Press Prev/Next to select a different parameter.
Press Save to save the new SOUR configuration.
Press ESC to leave without changing.
Parameters:
• Min time - the minimum time for the SOUR test.
Range: 1 to 3600 seconds.
• Max time - the maximum time for the SOUR test. The test will stop automatically when the
maximum time has elapsed.
Range: 1 to 3600 seconds.
• Min start DO - the minimum accepted DO value for starting the SOUR test. If the DO reading is
less than this value the test cannot be started.
Range: 0.01 to 50.00 mg/L.
• Min end DO - the minimum accepted DO value at the end of the test. If the DO reading at the
end of the SOUR test is less than this value a warning message will be displayed.
Range: 0.00 to 50.00 mg/L.
• Total volume - the volume of the diluted mixture.
Range: 0.1 to 300.0 mL
• Sample volume - the volume of sample in the diluted mixture.
Range: 0.1 to 300.0 mL.
• Solids weight: Total solids or Volatile suspended solids weight.
Range: 0.1 to 300.0 g/L.
• SOUR @ 20 °C: If this option to enabled the SOUR value is corrected to 20 °C.