Hettich ROTIXA 500 RS User manual

Rev. 01 / 11.15 Andreas Hettich GmbH & Co. KG AB4950DEENFRIT
ROTIXA 500 RS
DE
Bedienungsanleitung...................................................... 9
EN
Operating Manual............................................................ 36
FR
Mode d'emploi................................................................. 63
IT
Istruzioni per l'uso .......................................................... 90

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Andreas Hettich GmbH & Co. KG
Föhrenstraße 12, D-78532 Tuttlingen / Germany
Phone [phone] / 705-0
Fax [phone]
[email protected], [email protected]
www.hettichlab.com
0482
© 2014 by Andreas Hettich GmbH & Co. KG
All rights reserved. No part of this publication may be reproduced without the prior written permission of the copyright
owner.
Änderungen vorbehalten! , Modifications reserved! , Sous réserve de modifications ! , Con riserva di modifiche!
AB4950DEENFRIT / Rev. 01 / 11.15

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The following additional European directives and ordinances have been applied:
x Machinery Directive 2006/42/EC
x EMC directive 2004/108/EC
x Low voltage directive 2006/95/EC
x RoHS II Directive 2011/65/EC (without involvement of a notified body)
x Ordinance (EC) No. 1907/2006 (REACH) (without involvement of a notified body)
Applied standards:
According to the list of applied standards, which is part of the product file.
Les autres directives et normes européennes suivantes ont été appliquées:
x Directive 2006/42/EG relative aux machines
x Directive CEM 2004/108/EG
x Directive basse tension 2006/95/EC
x Directive RoHS II 2011/65/CE (sans participation d'un organisme désigné)
x Directive (CE) no. 1907/2006 (REACH) (sans participation d'un organisme désigné)
Normes appliquées:
Conformément à la liste des normes appliquées relatives aux cycles du produit.
Sono state applicate le seguenti direttive e regolamenti europei:
x Direttive per macchine 2006/42/CE
x Direttive per compatibilità elettromagnetica 2004/108/CE
x Direttive per basse tensioni 2006/95/CE
x RoHS II direttive 2011/65/CE (senza concorso di un citato collaboratore)
x Regolamento (CE) n. 1907/2006 (REACH) (senza concorso di un citato collaboratore)
Norme applicate:
Conformemente alla lista delle norme applicate, che sono parte degli atti del prodotto.
Letzte Gültigkeit dieser Erklärung:
Entsprechend dem letzten Gültigkeitsdatum der zum Zeitpunkt der Ausstellung dieser Erklärung gültigen EG-
Konformitätsbescheinigung, die durch die o.g. benannte Stelle nach Anhang V der Richtlinie 93/42/EWG ausgestellt
wurde: 2017-06-09
Last validity of this declaration:
In accordance with the last date of validity of the EC certificate of conformity as issued by the above-mentioned
notified body in accordance with appendix V of Directive 93/42/EEC which was valid at the time of the issuance of
this declaration: 2017-06-09
Dernière validité de cette déclaration:
Conformément à la dernière date de validité de l'attestation de conformité CE valide au moment où elle a été établie
par la société mentionnée ci-dessus conformément à l'annexe V de la directive 93/42/CEE : 2017-06-09
Ultima validità di questa dichiarazione:
Conformemente all'ultima data di validità del certificato di conformità CE valido al momento della pubblicazione di
questa dichiarazione, che è stata esposta tramite l'ufficio sopra citato come da appendice V delle direttive
93/42/CEE: 2017-06-09
Tuttlingen, 2015-11-24
Klaus-Günter Eberle
Geschäftsführer, Manager,
Directeur, Gerente

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Standards and regulations which apply to this device
The device is a high-end technical product. It is subject to extensive testing and certification procedures according to
the following standards and regulations in their respectively valid version:
Electrical and mechanical safety for design and final testing:
Standard series: IEC 61010 (conform to standards of DIN EN 61010)
x IEC 61010-1 “Safety requirements for electrical equipment for measurement, control, and laboratory use -
Part 1: General requirements” (Pollution Degree 2, Excess-voltage category II)
x IEC 61010-2-010 “Safety requirements for electrical equipment for measurement, control and laboratory use
- Part 2-010: Particular requirements for laboratory equipment for the heating of materials” (applied to
heated centrifuges only)
x IEC 61010-2-020 “Safety requirements for electrical equipment for measurement, control, and laboratory
use - Part 2-020: Particular requirements for laboratory centrifuges”
x IEC 61010-2-101 ”Safety requirements for electrical equipment for measurement, control and laboratory use
- Part 2-101: Particular requirements for in vitro diagnostic (IVD) medical equipment“
Electromagnetic Compatibility:
x EN 61326-1 “Electrical equipment for measurement, control and laboratory use - EMC requirements - Part 1:
General requirements“
Risk management:
x DIN EN ISO 14971 “Application of risk management to medical devices”
Restriction of Hazardous Substances (RoHS II):
x EN 50581 "Technical documentation for assessing electric and electronic devices with regard to the
restriction of hazardous substances"
European directives applied for conformity assessment procedures:
x Medical device directive 93/42/EEC (class 2a medical products)
EC conformity assessment procedure according to annex V
“EC DECLARATION OF CONFORMITY (production quality assurance)“ and annex VII
“EC DECLARATION OF CONFORMITY (self-declaration by the manufacturer)“.
The annex V certificate is issued by following notified body:
MEDCERT GmbH – Notified Body CE 0482
tel: +49 (0)40 - 22633250
fax: +49 (0)40 - 226332515
e-mail: [email protected]
website: www.med-cert.com
mail: Pilatuspool 2, D-20355 Hamburg; Germany
x Directive 2011/65/EU for the restriction of use of certain hazardous substances in electric and electronic
devices. Carrying out the EC conformity assessment process is the sole responsibility of the manufacturer,
without the involvement of a notified body.
Applied medical device regulations outside Europe:
x USA: QSR, 21CFR 820 “CFR Title 21 - Food and Drugs: TITLE 21- FOOD AND DRUGS, CHAPTER I -
FOOD AND DRUG ADMINISTRATION DEPARTMENT OF HEALTH AND HUMAN SERVICES,
SUBCHAPTER H - MEDICAL DEVICES, Part 820 QUALITY SYSTEM REGULATONS“
x Canada: CMDR, SOR/98-282 “Medical Devices Regulations”
Certified quality management system according to
x ISO 9001 “Quality management systems – Requirements”
x ISO13485 “Medical devices - Quality management systems - Requirements for regulatory purposes”
Environmental management system according to
x ISO 14001 “Environmental management systems - Requirements with guidance for use”

EN
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Contents
1 How to use these operating instructions...............................................................................................................38
2 Symbol meanings.................................................................................................................................................38
3 Intended use.........................................................................................................................................................39
4 Remaining risks....................................................................................................................................................39
5 Technical specifications........................................................................................................................................40
6 Notes on safety.....................................................................................................................................................42
7 Unpacking and setting up the centrifuge...............................................................................................................44
8 Delivery checklist..................................................................................................................................................44
9 Transport and storage ..........................................................................................................................................45
9.1 Transport.......................................................................................................................................................45
9.2 Storage..........................................................................................................................................................45
10 Initial operation..................................................................................................................................................45
11 Interface (only for centrifuge with interface) ......................................................................................................46
12 Opening and closing the lid...............................................................................................................................46
12.1 Opening the lid...........................................................................................................................................46
12.2 Closing the lid ............................................................................................................................................46
13 Installation and removal of the rotor..................................................................................................................46
14 Loading the rotor...............................................................................................................................................47
15 Closing biosafety systems.................................................................................................................................48
16 Control and display devices ..............................................................................................................................49
16.1 Status symbols...........................................................................................................................................49
16.2 Control knob...............................................................................................................................................49
16.3 Keys and set-up options ............................................................................................................................49
17 Entering centrifugation parameters ...................................................................................................................50
17.1 Running time..............................................................................................................................................50
17.1.1 Time selection.....................................................................................................................................50
17.1.2 Continuous running.............................................................................................................................50
17.2 Speed (RPM).............................................................................................................................................50
17.3 Relative centrifugal force (RCF/RZB).........................................................................................................50
17.4 Run-up and run-down parameters.............................................................................................................50
17.4.1 Run-up step........................................................................................................................................50
17.4.2 Run-up time........................................................................................................................................50
17.4.3 Braking step........................................................................................................................................51
17.4.4 Run-down time....................................................................................................................................51
17.4.5 Brake de-energisation speed..............................................................................................................51
17.5 Radius/Temperature ..................................................................................................................................51
17.5.1 Radius.................................................................................................................................................51
17.5.2 Temperature.......................................................................................................................................51
17.6 Automatic buffer.........................................................................................................................................51
18 Programming.....................................................................................................................................................51
18.1 Entering / modifying programs...................................................................................................................51
18.2 Calling up programs...................................................................................................................................51
19 Centrifugation....................................................................................................................................................52

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19.1 Centrifugation with time selection ..............................................................................................................52
19.2 Continuous running....................................................................................................................................52
20 Changing settings during the centrifugation run................................................................................................52
21 Integral RCF (RCF)..........................................................................................................................................52
22 Displaying the maximum speed of the rotor ......................................................................................................52
23 Displaying the maximum RCF of the rotor.........................................................................................................53
24 Emergency stop ................................................................................................................................................53
25 Audible signal....................................................................................................................................................53
26 Interrogating the operating hours ......................................................................................................................53
27 Setting the date and time ..................................................................................................................................53
28 Centrifugation data displayed after switching on...............................................................................................53
29 Immediate display of the centrifugation data after switching on ........................................................................54
30 Key-operated switch..........................................................................................................................................54
31 Program linking (for centrifuges with program linking only)...............................................................................54
31.1 Linking programs .......................................................................................................................................54
31.2 Changing program links.............................................................................................................................54
31.3 Centrifugation run with program linking......................................................................................................55
31.4 Deleting program linking ............................................................................................................................55
32 Cooling..............................................................................................................................................................55
32.1 Standby-cooling .........................................................................................................................................55
32.2 Pre-cooling the rotor...................................................................................................................................55
33 Heating..............................................................................................................................................................55
34 Relative centrifugal force (RCF)........................................................................................................................56
35 Centrifugation of materials or mixtures of materials with a density higher than 1.2 kg/dm3...............................56
36 Rotor recognition...............................................................................................................................................56
37 Emergency release ...........................................................................................................................................57
38 Maintenance and servicing................................................................................................................................57
38.1 Centrifuge (housing, lid and centrifuging chamber)....................................................................................57
38.1.1 Surface cleaning and care ..................................................................................................................57
38.1.2 Surface disinfection.............................................................................................................................58
38.1.3 Removal of radioactive contaminants.................................................................................................58
38.2 Rotors and Attachments.............................................................................................................................58
38.2.1 Cleaning and care...............................................................................................................................58
38.2.2 Disinfection .........................................................................................................................................59
38.2.3 Removal of radioactive contaminants.................................................................................................59
38.2.4 Trunnions............................................................................................................................................59
38.2.5 Rotors and accessories with limited service lives ...............................................................................59
38.2.6 Calculation of the number of run cycles carried out............................................................................59
38.3 Autoclaving ................................................................................................................................................60
38.4 Centrifuge containers.................................................................................................................................60
39 Faults ................................................................................................................................................................61
40 Returning Devices.............................................................................................................................................62
41 Disposal ............................................................................................................................................................62
42 Anhang / Appendix..........................................................................................................................................117
42.1 Rotoren und Zubehör / Rotors and accessories.......................................................................................117

EN
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1 How to use these operating instructions
x Before using the centrifuge, read the operating instructions and observe them.
x The operating instructions are a part of the device. They must always be kept readily available.
x If the device is set up at a different location, the operating instructions must be provided with it.
2 Symbol meanings
Symbol on the device:
Attention, general hazard area.
Before using the device, make sure you read the operating instructions and observe the safety
information!
Symbol in this document:
Attention, general hazard area.
This symbol refers to safety relevant warnings and indicates possibly dangerous situations.
The non-adherence to these warnings can lead to material damage and injury to personal.
Symbol on the device and in this document:
Beware of biohazard.
Symbol on the device and in this document:
Beware of hot surface.
Nonobservance of this warning can lead to material damage and personal injury.
Symbol on the device:
Equipotential: Connector (PE connector) for potential equalization (only for centrifuge with PE
connector).
Symbol on the device:
Switch positions of the key-operated switch.
IOIOI
OPTICAL
Symbol on the device:
Optical interface (only for device with optical interface).
Symbol in this document:
This symbol refers to important circumstances.
Symbol on the device and in this document:
Symbol for the separate collection of electric and electronic devices according to the guideline
2002/96/EG (WEEE). The device belongs to Group 8 (medical devices).
Applies in the countries of the European Union, as well as in Norway and Switzerland.

EN
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3 Intended use
This device is a laboratory centrifuge which is suitable for medical and in-vitro diagnostic applications.
The therapeutic purpose with the corresponding centrifuge vessels is the separation of blood by means of
centrifugation for the purpose of transfusions or autotransfusion of the won blood components.
The centrifuge is also suitable for preparing human bodily fluids by means of centrifugation for in-vitro diagnostic
purposes.
Furthermore, the centrifuge is generally also suitable for separating substances or substance mixtures of non-human
origin having a density of max. 1.2 kg/dm³.
The centrifuge may only be used by skilled personnel in closed laboratories.
The centrifuge is only meant for the above-named purposes.
Another use or one which goes beyond this, is considered to be non-intended. The company Andreas Hettich GmbH
& Co. KG is not liable for damage resulting from this.
Observing all information in the operating instructions and complying with the measures described therein is also a
part of the intended use.
4 Remaining risks
The device is built according to the state-of-the-art and the recognized safety regulations.
If used and handled improperly, there could be life-threatening danger to the user or third parties, or the device could
be impaired or there could be other property damage.
The device is only to be used for its intended purpose and only when it is in safe working condition.
Malfunctions which could affect safety must be corrected immediately.

EN
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5 Technical specifications
Manufacturer Andreas Hettich GmbH & Co. KG
D-78532 Tuttlingen
Model ROTIXA 500 RS
Type 4950,
4950-50 4950-70, 4950-80
Mains voltage (r10%) 230-240 V a220 V a230-240 V a220 V a
Mains frequency 50 Hz 60 Hz 50 Hz 60 Hz
Connected load 3800 VA
Current consumption 16 A
Cooling medium R 404A
Max. capacity 4 x 1000 ml
Allowed density 1.2 kg/dm3
Speed (RPM) 11500
Force (RCF) 18038
Kinetic energy 59620 Nm
Obligatory inspection (BGR 500) yes
Ambient conditions (EN / IEC 61010-1)
 Set-up site Indoors only
 Altitude Up to 2000 m above sea level
 Ambient temperature 5°C to 35°C
 Humidity Maximum relative humidity 80% for temperatures up to 31°C, linearly
decreasing to 50% relative humidity at 40°C.
 Excess-voltage category
(IEC 60364-4-443) ,,
 Pollution degree 2
Device protection class ,
Not suitable for use in explosion-endangered areas.
EMC
 Emitted interference,
Interference immunity EN / IEC 61326-1, Class B
Noise level (dependent on rotor) d65 dB(A) d63 dB(A)
Dimensions
 Width 650 mm
 Depth 814 mm
 Height 973 mm
Weight approx. 219 kg approx. 233 kg

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Manufacturer Andreas Hettich GmbH & Co. KG
D-78532 Tuttlingen
Model ROTIXA 500 RS
Type 4950-08,
4950-58 4950-78, 4950-88
Mains voltage (r10%) 208 V a
Mains frequency 60 Hz
Connected load 3800 VA
Current consumption 18 A
Cooling medium R 404A
Max. capacity 4 x 1000 ml
Allowed density 1.2 kg/dm3
Speed (RPM) 11500
Force (RCF) 18038
Kinetic energy 59620 Nm
Obligatory inspection (BGR 500) yes
Ambient conditions (EN / IEC 61010-1)
 Set-up site Indoors only
 Altitude Up to 2000 m above sea level
 Ambient temperature 5°C to 35°C
 Humidity Maximum relative humidity 80% for temperatures up to 31°C, linearly
decreasing to 50% relative humidity at 40°C.
 Excess-voltage category
(IEC 60364-4-443) ,,
 Pollution degree 2
Device protection class ,
Not suitable for use in explosion-endangered areas.
EMC
 Emitted interference,
Interference immunity FCC Class B
Noise level (dependent on rotor) d65 dB(A) d63 dB(A)
Dimensions
 Width 650 mm
 Depth 814 mm
 Height 973 mm
Weight approx. 225 kg approx. 239 kg

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6 Notes on safety
No claim of warranty will be considered by the manufacturer unless ALL instructions in this manual
have been followed.
x The centrifuge should be installed on a good, stable base.
x Before using the centrifuge absolutely check the rotor for firm placement.
x When the centrifuge is running, according to EN / IEC 61010-2-020, no persons, dangerous
substances or objects may be within the safety margin of 300 mm around the centrifuge.
x Rotors, suspensions and accessories that possess traces of corrosion or mechanical damage or
if their term of use has expired may not be used any longer.
x The centrifuge may no longer be put into operation when the centrifuging chamber has safety-
related damages.
x With swing-out rotors the trunnions must be regularly lubricated (Hettich Lubricating Grease No.
4051) in order to ensure consistent swinging out of the hangers.
x For centrifuges without temperature control, when the room temperature is increased and/or if the
device is frequently used, the centrifuging chamber could be heated up. Therefore, it can't be
ruled out that the sample material might be changed due to the temperature.
x Before the initial operation of your centrifuge you should read and pay attention to the operating
instructions. Only personnel that has read and understood the operating instructions are allowed to
operate the device.
x Along with the operating instructions and the legal regulations on accident prevention, you should also follow the
recognised professional regulations for working in a safe and professional manner. These operating instructions
should be read in conjunction with any other instructions concerning accident prevention and environmental
protection based on the national regulations of the country where the device is to be used.
x This centrifuge is a state-of-the-art piece of equipment which is extremely safe to operate. However, it can lead to
danger for users or others if used by untrained staff, in an inappropriate way or for a purpose other than that it
was designed for.
x The centrifuge must not be moved or knocked during operation.
x In case of fault or emergency release, never touch the rotor before it has stopped turning.
x To avoid damage due to condensate, when changing from a cold to a warm room the centrifuge must either heat
up for at least 3 hours in the warm room before being connected to the mains, or run hot for 30 minutes in the
cold room.
x Only the rotors and accessories approved by the manufacturer for this device may be used (see chapter
"Anhang/Appendix, Rotoren und Zubehör/Rotors and accessories"). Before centrifuge vessels are used which are
not listed in the chapter "Appendix, Rotors and accessories", the user must make sure they can be used by
asking the manufacturer.
x The centrifuge rotor may only be loaded in accordance with the chapter "Loading the rotor".
x When centrifuging with maxim revolutions per minute the density of the materials or the material mixtures may not
exceed 1.2 kg/dm3.
x The centrifuge may only be operated when the balance is within the bounds of acceptability.
x The centrifuge may not be operated in explosion-endangered areas.
x The centrifuge must not be used with:
 inflammable or explosive materials
 materials that react with one another producing a lot of energy.

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x If users have to centrifuge hazardous materials or compounds contaminated with toxic, radioactive or pathogenic
micro-organisms, they must take appropriate measures.
For hazardous substances centrifuge containers with special screw caps must strictly be used. In addition to the
screw cap centrifuge containers, for materials in hazard category 3 and 4 a biosafety system must be used (see
the World Health Organisation’s “Laboratory Biosafety Manual”).
In a biosafety system, droplets and aerosols are prevented from escaping by a bioseal (packing ring).
If the hanger of a biosafety system is used without the lid, the packing ring must be removed from the hanger in
order to prevent the packing ring from being damaged during the centrifugation run. Damaged packing rings must
not be used to seal the biosafety system.
Without the use of a biosafety system the centrifuge is not microbiologically sealed in the sense of the
EN / IEC 610101-2-020 standard.
When closing a biosafety system, please observe the instructions provided in the chapter, "Closing Biosafety
Systems".
For further details of available biosafety systems see chapter "Anhang/Appendix, Rotoren und Zubehör/Rotors
and accessories". If in doubt, you should obtain relevant information from the manufacturer.
x The centrifuge must not be operated with highly corrosive substances which could impair the mechanical integrity
of rotors, hangers and accessories.
x Repairs must only be carried out by personnel authorised to do so by the manufacturer.
x Only original spare parts and original accessories licensed by the Andreas Hettich GmbH & Co. KG company are
allowed to be utilised.
x The following safety regulations apply:
EN / IEC 61010-1 and EN / IEC 61010-2-020 as well as their national deviations.
x The safe operation and reliability of the centrifuge can only be guaranteed if:
 the centrifuge is operated in accordance with the operating instructions,
 the electrical installation on the site where the centrifuge is installed conforms to the demands of EN / IEC
stipulations,
 the tests for device safety required in the respective countries, e.g. in Germany in acc. with BGV A1 and BGR
500, are carried out by an expert.

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7 Unpacking and setting up the centrifuge
a
c
be
c
fd
x Remove the packaging.
x Remove the front wooden beam (a).
x Fasten the metal rails (b) to the wooden pallet with two nails each.
x Push the front wooden beam (a) under the metal rails (b) to support these.
x Roll the centrifuge carefully down from the wooden pallet using the metal rails (b).
x Push the centrifuge to its set-up location.
x Place the open-end wrench included in delivery (size 10 mm) on the surfaces (d) and turn the device feet (c)
downward until the castor wheels (f) no longer have contact with the floor.
x Align the centrifuge horizontally by turning the device feet (c).
x Turn the hexagon nuts (e) upward with the open-end wrench included in delivery (size 19 mm) and tighten to
secure the position of the device feet (c).
8 Delivery checklist
1 Power cord
1 Single open-end wrench, size 10 mm (for turning the device feet)
1 Double open-end wrench, size 17/19 mm (for tightening the hexagon nuts on the device feet)
1 Square wrench (for installing and removing the rotor)
1 Allen key (to unlock the lid in an emergency)
1 Lubricating grease for trunnions
1 Tube emptier
10 Cover caps (12 mm) for Torx screw heads, size T20
1 Operating manual
3 Program-data sheets
The rotor(s) and associated accessories are included in the delivery in the quantity ordered.

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9 Transport and storage
9.1 Transport
When the device and accessories are transported, the following ambient conditions must be complied with:
x Ambient temperature: -20°C to +60°C
x Relative humidity: 20% to 80%, non-condensing
9.2 Storage
The device and the accessories may only be stored in closed and dry rooms.
When the device and accessories are stored, the following ambient conditions must be complied with:
x Ambient temperature: -20°C to +60°C
x Relative humidity: 20% to 80%, non-condensing
10 Initial operation
We recommend installing an Emergency Stop switch in the building installation to disconnect the mains
power supply in the event of an error.
x Only for types 4950-08, 4950-58, 4950-78 and 4950-88:
According to the laboratory device standard EN / IEC 61010-1, there must be a switch installed in the building
installation to disconnect the power to the device.
This switch has to be placed close to the centrifuge, must be easily reachable for the user and has to be
characterized as a separator for this unit. The switch must also be able to be secured against being switched on
again.
x Types 4950-70, 4950-78, 4950-80 and 4950-88 may only be installed by authorized skilled personnel.
These devices must be installed according to the enclosed set-up and installation instructions,
AH4950EN.
x Position the centrifuge in a stable and level manner in a suitable place. During set-up, the required safety
margin of 300 mm around the centrifuge is to be kept according to EN / IEC 61010-2-020.
When the centrifuge is running, according to EN / IEC 61010-2-020, no persons, dangerous
substances or objects may be within the safety margin of 300 mm around the centrifuge.
x Ventilation openings may not be blocked.
A distance of 300 mm must be maintained from the ventilation slots and openings of the centrifuge.
x Centrifuge with PE connector:
If required, connect the PE connector at the back of the device with an additional medical potential equalization
system.
x Centrifuge with optical interface:
Connect the optical centrifuge interface with a light-conducting cable (not included in the scope of delivery) to the
PC.
x Check whether the mains voltage tallies with the statement on the type plate.
x Connect the centrifuge to a standardized mains socket with the power cord. For connection ratings refer to
Chapter "Technical specification".
x Turn on the mains switch. Switch position "
,
".
The following display follows:
1. Type of centrifuge, 2. The last rotor code detected by the rotor identification and the maximum speed of the
rotor (n-max-Rotor), 3.The programme version, 4. OPEN OEFFNEN.
x Open the lid.
The centrifuge data of the last used program or program 1 will be displayed.

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11 Interface (only for centrifuge with interface)
As an option, the device can be equipped with an optical interface.
The optical interface is labeled with the
IOIOI
OPTICAL
symbol.
The centrifuge can be controlled and data queried via this interface.
12 Opening and closing the lid
12.1 Opening the lid
The lid can only be opened when the centrifuge is switched on and the rotor is at rest. If it cannot be
opened under these circumstances, see the section on “Emergency release”.
x Press the button on the front panel. The lid unlocks via the motor, the illuminated pushbutton extinguishes
and in the display the symbol changes to the symbol .
12.2 Closing the lid
Do not put your fingers between lid and housing.
Do not bang the lid shut.
x Place the lid and slightly press it down at the tubular handle. The locking action is effected by motor. The button
lights up and the symbol in the display turns to the symbol .
13 Installation and removal of the rotor
B
C
D
A
x Clean the motor shaft (C) and the rotor drilling (A), and lightly grease the motor shaft
afterwards. Dirt particles between the motor shaft and the rotor hinder a perfect seating
of the rotor and cause an irregular operation.
x Place the rotor vertically on the motor shaft. The motor shaft dog (D) has to fit in the
rotor slot (B). The alignment of the groove is labelled on the rotor.
x Tighten the rotor tension nut with the supplied wrench by turning in a clockwise
direction.
x Check the rotor for firm seating.
x Loosening the rotor: Loosen the tension nut by turning in a counter clockwise direction,
and turning until the working point for lifting. After passing the working point for lifting
the rotor is loosened from the motor shaft cone. Turn the tension nut until the rotor is
able to be lifted from the motor shaft.

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14 Loading the rotor
Standard centrifuge containers of glass will not stand RCF values exceeding 4000 (DIN 58970, pg. 2).
x Check the rotor for firm seating.
x With swing-out rotors all rotor positions must be lined with identical hangers. Certain hangers are marked with
the number of the rotor position. These hangers may only be used in the respective rotor position. Hangers that
are marked with a set number (e.g. S001/4) may only be used in the set.
x The rotors and hangers may only be loaded symmetrically. The centrifuge containers have to be distributed
evenly on all rotor positions. For authorised combinations see Chapter "Anhang/Appendix, Rotoren und
Zubehör/Rotors and accessories".
In the case of angle rotors all possible rotor positions must be loaded, see chapter "Anhang/Appendix, Rotoren
und Zubehör/Rotors and accessories".
Rotor is evenly loaded Not permitted!
Rotor is not evenly loaded
x On certain hangers, the weight of the maximum load or the weight of the maximum load and the maximum
weight of the completely loaded hanger is specified. This weight may not be exceeded. In case of exception, see
chapter " Centrifugation of materials or mixtures of materials with a density higher than 1.2 kg/dm3".The weight
specified for the maximum loading includes the total weight of adapter, frame, centrifuging container and content.
x In containers with rubber inserts, the same number of rubber inserts must always be among the centrifuge
containers.
x The centrifuge containers may only be filled outside of the centrifuge.
x The maximum filling quantity for the centrifuge containers specified by the manufacturer must not be exceeded.
In the case of angle rotors, the centrifuging
vessels may only be filled so far that no fluid
can be expelled from them while the centrifuge
is running.
Fluid
Centrifugal force
x When loading the angle rotors, no liquid may get into the angle rotors or centrifuging chamber.
x When loading the hangers and swing-out rotors and when swivelling out the hangers while the centrifuge is
running, no liquid may enter the hangers or the centrifuging chamber.
x In order to maintain the weight differences within the centrifuge container as marginal as possible, a consistent
fill level in the containers is to be heeded.

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15 Closing biosafety systems
In order to guarantee that it is sealed, the lid to the biosafety system has to be firmly closed.
In order to prevent the packing ring from twisting when opening and closing the cover, the packing ring must
be lightly rubbed with talcum powder or a rubber care product.
For further details of available biosafety systems see chapter "Anhang/Appendix, Rotoren und Zubehör/Rotors
and accessories". If in doubt, you should obtain relevant information from the manufacturer.
Lid with screw top and hole in turning handle:
x Insert the supplied key into the hole in the handle and close the lid firmly by turning the key in
a clockwise direction.
Lid with screw top:
x Close the lid firmly by hand by turning in clockwise direction.

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16 Control and display devices
LOCK1
STOP
120:30
400020022
PC
16.1 Status symbols
Lid open.
Lid closed.
Rotation indicator. The indication appears while the centrifuge is running as long as the rotor is turning.
STOP Centrifugation run stopped or finished. Indication after completion of the centrifugation run as long as the
rotor is turning. The indicator flashes following an emergency stop.
LOCK 1,
LOCK 2 Keyswitch position.
LOCK 4,
LOCK 5 Program interlocking when serial communication provided (for centrifuges with serial communication only).
PC,
P
C
Serial communication (for centrifuges with serial communication only).
Operating errors or faults that occur are shown symbolically in the display (see Chapter "Faults").
16.2 Control knob
For setting the individual parameters.
Turning anticlockwise reduces the value. Turning clockwise increases the value.
16.3 Keys and set-up options
t
Running time, parameter t/min:sec
1. Parameter t/min: Adjustable from 1 - 999 min, in 1 min steps.
2. Parameter t/ :secAdjustable from 1 - 59 s, in 1 second steps.
3. Continuous running "---:--"
RCF
Interrogation of the integral RCF, parameter RCF.
n
1. Speed, parameter RPM
A numerical value from 50 RPM up to the maximum speed of the rotor (n-max-Rotor) can be set. Maximum
speed of the rotor, see Chapter "Anhang/Appendix, Rotoren und Zubehör/Rotors and accessories".
Adjustable in steps of 10.
2. Interrogation of the maximum speed of the rotor, parameter n-max-Rotor.
RCF
1. Relative centrifugal force, parameter RCF/RZB
A numerical value can be set, which gives a speed between 50 RPM and the maximum speed of the rotor
(n-max-Rotor). Adjustable in steps of 1.
2. Interrogation of the maximum RCF of the rotor, parameter RCF-max-Rotor.
Run-up parameters
1. Run-up steps, parameter 1-9. Step 9 = shortest run-up time, ... Step 1 = longest run-up time.
2. Run-up time, parameter
min:se
c
t
. The time range that can be set is dependent upon the set speed.
Run-down parameters
1. Braking steps, parameter 0-9. R = linear braking curve, B = similar to an exponential braking curve.
Step R9, B9 = short run-down time, ... Step R1, B1 = long run-down time, Step R0 = unbraked run-down.
2. Run-down time, parameter
min:se
c
t
. The time range that can be set is dependent upon the set speed.
3. Brake de-energisation speed, parameter n(•)/RPM
After reaching this speed, unbraked run-down occurs.
1. Temperature setpoint, parameter T/°C. Adjustable from -20°C to +40°C, in 1°C steps (heating/cooling
option settable between -20°C and +60°C). The lowest temperature that can be achieved is dependent
upon the rotor (see Chapter "Anhang/Appendix, Rotoren und Zubehör/Rotors and accessories").
2. Centrifuging radius, parameter r/mm. Entry in mm. Centrifuging radius, see Chapter "Anhang/Appendix,
Rotoren und Zubehör/Rotors and accessories“.

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START
1. Start centrifugation run. The rotation indicator appears.
2. Acceptance of changes during the centrifugation run.
STOP
Stop centrifugation run. The rotor decelerates with the preset run-down parameters. Pressing the key twice will initiate an emergency
stop.
PROG
Select program location, parameter PROG-Nr.
RCL
Calling up of programs.
STO
Saving of programs.
89 programs can be saved (program locations 1 to 89). Note: The program locations "----" and 90 to 99 are
used as automatic buffers (see Chapter "Automatic buffer"). No programs can be saved in these program
locations.
17 Entering centrifugation parameters
It is only possible to enter a parameter when the input field is displayed inversely (dark background). An
inverse input field will go off automatically after 10 seconds.
17.1 Running time
17.1.1 Time selection
x Press the
t
key repeatedly until the parameter t/min: or t/ :sec is displayed. The input field will be displayed
inversely.
x Set the desired value by means of the control knob .
17.1.2 Continuous running
x Select the parameters t/min: and t/ :sec one after the other (see Chapter "Time selection"), and set both to zero
using the control knob . "---:--" will be displayed in the input field.
17.2 Speed (RPM)
x Press the
n
key repeatedly until the parameter RPM is displayed and the input field is shown inversely.
x Set the desired value by means of the control knob .
17.3 Relative centrifugal force (RCF/RZB)
x Press the
RCF
key repeatedly until the parameter RCF/RZB is displayed and the input field is shown inversely.
x Set the desired value by means of the control knob .
The centrifuging radius indicator will flash while the adjustment is being made.
17.4 Run-up and run-down parameters
The run-up and run-down parameters that have been set are displayed.
x: 1-9 = run-up step, t = run-up time
xy
PROFIL y: R1-R9, B1-B9 = braking step, R0 = unbraked run-down, t = run-down time, n(•) = brake de-
energisation speed
17.4.1 Run-up step
x Press the key repeatedly until the parameter 1-9 or
t
is displayed. The input field will be displayed
inversely.
x Set the desired step by means of the control knob .
17.4.2 Run-up time
x Press the key repeatedly until the parameter
min:se
c
t
is displayed. The input field will be displayed inversely.
x Set the desired value by means of the control knob .
If a run-up time is set that is longer than the running time, the centrifugation run will end before the set speed is
reached.

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17.4.3 Braking step
x Press the key repeatedly until the parameter 0-9 or
t
is displayed. The input field will be displayed
inversely.
x Set the desired step by means of the control knob .
B-braking steps can only be set for special rotors.
17.4.4 Run-down time
If a brake de-energisation speed has been set, it is not possible to set a run-down time.
x Press the key repeatedly until the parameter
min:se
c
t
is displayed. The input field will be displayed inversely.
x Set the desired value by means of the control knob .
17.4.5 Brake de-energisation speed
x Press the key repeatedly until the parameter n(•)/RPM is displayed. The input field will be displayed inversely.
x Set the desired value by means of the control knob .
17.5 Radius/Temperature
17.5.1 Radius
x Press the key repeatedly until the parameter r/mm is displayed and the input field is shown inversely.
x Set the desired value by means of the control knob .
The value of the RCF/RZB adapts automatically when the radius is changed; this is shown by the indication flashing.
17.5.2 Temperature
x Press the key repeatedly until the parameter T/°C is displayed and the input field is shown inversely.
x Set the desired value by means of the control knob .
17.6 Automatic buffer
The buffer comprises the program locations "----" and 90 to 99. Every time a centrifugation run is started, modified
centrifugation data are automatically stored in program location "----". The modified centrifugation data for the last 11
centrifugation runs are stored in the buffer and can be called up (see Chapter "Calling up programs").
18 Programming
18.1 Entering / modifying programs
x Set the desired parameters (see Chapter "Entering centrifugation parameters").
x Press the
PROG
key in order to select the parameter PROG-Nr. The input field will be displayed inversely.
x Set the desired program location by means of the control knob .
If the program location indicator flashes, then this program location has already had centrifugation data assigned
to it. In this case, set a free program location or overwrite the centrifugation data by continuing.
x Press the
STO
key once to save the settings in the desired program location.
Press the
STO
key twice to overwrite centrifugation data already stored.
18.2 Calling up programs
x Press the
PROG
key in order to select the parameter PROG-Nr. The input field will be displayed inversely.
x Set the desired program location by means of the control knob .
x Press the
RCL
key. The centrifugation data for the selected program location will be displayed.