Hettich ZENTRIMIX 380 R User manual

ZENTRIMIX
380 R
Operating manual
Read the instructions prior to performing any task!
Translation of the original operating manual

Translation of the original operating manual
© 2017
Andreas Hettich GmbH & Co. KG
Föhrenstraße 12
78532 Tuttlingen
Germany
Telephone: [phone] / 705-0
Fax: [phone]
Email: [email protected]
Email: [email protected]
Internet: www.hettichlab.com
ZENTRIMIX, 380 R, en_GB, 2
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN
2

Table of contents
1 Introduction......................................................................... 5
1.1 Symbols........................................................................ 5
1.2 Personal protective equipment..................................... 8
1.3 Personnel qualification.................................................. 8
1.4 Intended use................................................................. 9
1.5 Replacement parts/consumable material................... 10
1.6 Scope of delivery........................................................ 10
1.7 Return shipments........................................................ 11
2 Safety information............................................................ 13
3Device description............................................................ 21
4 Transport and storage...................................................... 23
5 Commissioning................................................................. 25
5.1 Unpacking the device................................................. 25
5.2 Setting up, connecting and switching on the device... 26
6 Operation........................................................................... 31
6.1 Operating elements.................................................... 31
6.2 Opening the lid............................................................ 35
6.3 Closing the lid............................................................. 35
6.4 Quick-stop function..................................................... 35
6.5 Loading the rotor......................................................... 36
6.5.1 Loading a rotor for classical centrifugation.............. 36
6.5.2 Loading a rotor for dual centrifugation..................... 37
6.5.3 Handling of bio-safety systems................................ 38
6.6 Starting centrifugation................................................. 39
6.7 Dual centrifugation: Validating application.................. 41
6.8 Emergency unlocking................................................. 43
6.9 Removing and installing the rotor............................... 45
6.10 Cooling...................................................................... 47
7 Software description........................................................ 49
7.1 Program settings......................................................... 50
7.1.1 Calling up/loading program (RCL 1-99)................... 51
7.1.2 Entering or changing programs (STO 1-99)............ 52
7.1.3 Calling up/loading a program link (RCL A-Z)........... 53
7.1.4 Editing a program link (EDIT A-Z)............................ 54
7.1.5 Saving a program link (STO A-Z)............................. 55
7.2 Centrifugation parameters.......................................... 56
7.2.1 Temperature (t/°C)................................................... 57
7.2.2 Start-up and run-down parameters.......................... 57
7.2.3 RCF and RAD.......................................................... 59
7.2.4 Speed (RPM)........................................................... 61
7.2.5 Runtime (t/hms)....................................................... 62
7.2.6 Precooling of the rotor............................................. 65
Table of contents
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 3

7.3 Machine Menu............................................................ 66
7.3.1 Change Lock............................................................ 67
7.3.2 Change PIN............................................................. 68
7.3.3 Info........................................................................... 70
7.3.4 Operating Time........................................................ 71
7.3.5 Settings.................................................................... 74
8 Cleaning, disinfection and maintenance........................ 83
8.1 Cleaning...................................................................... 84
8.2 Disinfection................................................................. 85
8.3 Maintenance............................................................... 86
9 Troubleshooting................................................................ 89
9.1 Performing a mains reset............................................ 91
10 Technical data................................................................... 93
10.1 Type plate................................................................. 94
10.2 Dimensions............................................................... 95
11 Disposal............................................................................. 97
12 Glossary............................................................................. 99
13 Index................................................................................. 101
Appendix.......................................................................... 105
A Rotors and accessories............................................... 107
Table of contents
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 4

1 Introduction
1.1 Symbols
Signal word Meaning
DANGER! This combination of symbol and
signal word indicates an imme-
diate dangerous situation that
will result in death or serious
injury if it is not avoided.
WARNING! This combination of symbol and
signal word indicates a possible
dangerous situation that can
result in death or serious injury
if it is not avoided.
CAUTION! This combination of symbol and
signal word indicates a possible
dangerous situation that can
result in minor injury if it is not
avoided.
NOTICE! This combination of symbol and
signal word indicates a possible
dangerous situation that can
result in material and environ-
mental damage if it is not
avoided.
Warning signs Type of danger
Warning – hand injuries.
Warning – high-voltage.
Warning – danger zone.
This listing symbol denotes descriptions of tasks that you
must perform.
nThis dot is for denoting lists.
– This dash denotes listings of the second level.
Signal words
Warning categories
General symbols
Introduction
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 5

Cross references are indicated as follows:
Ä Chapter 1.1 ‘Sym-
bols’ on page 5
Warnings/symbols on the device which are no
longer recognizable should be immediately
replaced by the operating company.
The images shown in the following depict the posi-
tions of the warnings and symbols affixed to the
device.
Fig. 1: Warnings on the device
Attention, general danger point
Nonobservance of this warning can lead to material damage and
personal injury.
Before using the device, make sure you read the operating instruc-
tions and observe the safety information.
Warning - Biological contamination!
Before using the device, make sure you read the operating instruc-
tions and observe the safety information.
Warning - Hand injury
Nonobservance of this warning can lead to material damage and
personal injury.
Before using the device, make sure you read the operating instruc-
tions and observe the safety information.
Cross reference within the docu-
ment
Symbols on the device
Introduction
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 6

Fig. 2: Warning stickers on the right outer side
Attention, general danger point
Nonobservance of this warning can lead to material damage and
personal injury.
Before using the device, make sure you read the operating instruc-
tions and observe the safety information.
Symbol for separate collection of electric and electronic
devices
Symbol according to directive 2002/96/EC (WEEE). Applies in the
countries of the European Union, as well as in Norway and Swit-
zerland.
Type plate
Nameplate with technical data specifications.
Symbol for TÜV-inspected device
From TÜV-inspected device.
Introduction
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 7

IOIOI
RS232
IOIOI
RS232
Fig. 3: Notice at the RS232 interface
Symbol for RS232 interface
This symbol indicates the RS232 interface (only for device with
RS232 interface).
1.2 Personal protective equipment
The assessment of the personal protective equipment must take
place on site according to the actual risks which are posed by the
used substances, the procedures and the environmental condi-
tions.
The operating company must create a risk assessment according
to the corresponding standards and directives and enact work
instructions which ensure the safe handling of the device and
accessories.
1.3 Personnel qualification
Repairs may only be performed by a person authorized by the
manufacturer.
NOTICE!
Interventions and modifications to devices by per-
sons not authorized by the company Andreas Het-
tich GmbH & Co. KG will be at one's own risk and
result in the voiding of all warranty claims, as well
as the voiding of liability claims against Andreas
Hettich GmbH & Co. KG.
Authorized skilled personnel with special instruction
Skilled personnel are able to perform the work assigned to them
and independently recognize and avoid potential hazards based on
their specialized education (professionally recognized vocational
training), knowledge and experience as well as knowledge of the
relevant regulations.
IOIOI
RS232
Introduction
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 8

Personnel have participated in special instruction by the manufac-
turer of the device or personnel authorized by the manufacturer
and, through this, are authorized to perform the corresponding
task.
Personnel have completely read and understood this documenta-
tion.
Service technician
A service technician is someone who, based on his professional
training and advanced practical knowledge, provides expert sup-
port (installation, commissioning, maintenance, etc.) for our cus-
tomers in all things mechanical, on the phone as well as on site,
and is an employee of the company of the manufacturer or was
trained and authorized by the manufacturer to carry out service
work on the device.
1.4 Intended use
Dual centrifugation: The purpose of the ZentriMix is to process
samples pre-analytically in non-medical laboratories.
This includes the extraction, grinding, mixing and dissolving as well
as homogenizing of samples, so also the decomposition of organic
or inorganic materials.
Another purpose is the creation of nanoparticles through homoge-
nization (e.g. lipid nanoparticles, particularly liposomes) for non-
clinical uses.
Classical centrifugation: In its function as a classical centrifuge, it
is used for the separation of materials or material mixtures with a
density of max. 1.2 kg/dm³ in non-medical laboratories.
The device was not designed for use in environmental conditions
that do not meet the manufacturer requirements or in a potentially
explosive, radioactive or biologically or chemically contaminated
atmosphere.
The ZentriMix is only meant for the above-named purposes.
Another use or one which goes beyond this is considered to be
improper. The manufacturer is not liable for damage resulting from
this.
Intended use also includes the observation of all information in the
operating instructions and complying with the inspection and main-
tenance intervals.
The device was not designed for use under environmental condi-
tions that do not meet the manufacturer requirements or in a poten-
tially explosive, radioactive or biologically or chemically contami-
nated atmosphere.
The device is not meant for processing toxic, corrosive or infec-
tious samples; such samples must not be processed in the Zen-
triMix.
Intended use
Improper use
Introduction
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 9

The device is built according to the state-of-the-art and the recog-
nized safety regulations. If used and handled improperly, there
could be life-threatening danger to the user or third parties, or the
device could be impaired or there could be other property damage.
The device is only to be used for its intended purpose and only
when it is in safe working condition. Malfunctions which could
affect safety must be corrected immediately.
If consumable material approved/sold by the manufacturer is not
used, there is a danger of container damage at high speeds, which
could result in a spontaneously high unbalance and damage to the
device.
Do not operate the device unsupervised.
If used improperly, incorrect input of the parameters can result in
dangerous operating conditions: For example, if the duration of a
run is entered too high, the sample could overheat, which could
cause the sample container to burst, which could result in extreme
unbalance at high speed and damage to the device.
1.5 Replacement parts/consumable material
Only original replacement parts and approved original accessories
of the manufacturer may be used and only original consumable
material or that recommended (approved) by the manufacturer in
its official documentation may be used.
WARNING!
If original replacement parts, consumable material
approved or sold by the manufacturer and original
accessories are not used, any warranty or liability
claims against the company Andreas Hettich
GmbH & Co. KG are void.
1.6 Scope of delivery
n1 x device with mounted rotor
n1 x mains cable (power supply)
n1 x size 5 Allen wrench (for emergency release and mounting/
dismounting the rotor)
n1 x lubricating grease for lifting lugs
n1 x operating instructions
Rotor(s) and the corresponding accessories are
included in delivery, depending on the order.
Foreseeable misuse
Introduction
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 10

1.7 Return shipments
If the device or its accessories are sent to the manufacturer, this/
these must be decontaminated and cleaned before shipping in
order to protect personnel, the environment and material. Please
contact the Service department of the manufacturer for the return
shipment. You will then receive a clearance certificate and return
material authorization number. Devices without a signed clear-
ance certificate will not be accepted in order to protect our
employees. We ask for your understanding.
Introduction
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 11

Introduction
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 12

2 Safety information
The device and its associated components and
assemblies meet the currently valid safety stand-
ards individually and as a whole and meet the CE
directives of the European Union.
The device is safe if used as intended and
observing the descriptions and information given in
this documentation.
WARNING!
Warning of incorrect voltage on the device pro-
tection switch!
Pay attention to the correct operating voltage in
order to prevent damage to the device.
–Only operate the device at the correct oper-
ating voltage.
– Refer to the technical data and nameplate for
the value for the correct operating voltage.
– If the operating voltage is too high, the overcur-
rent protection fuse in the device switch can
trip and the device electronics can be
destroyed.
DANGER!
Danger due to rotor moving with high kinetic
energy!
There is a risk of injury if the lid is opened via the
emergency release during device operation.
– Only open the device via the emergency
release when the device is switched off.
–Before the emergency release, make sure that
the device rotor has stopped.
Danger due to electrical energy
Danger due to kinetic energy
Safety information
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 13

DANGER!
Danger due to insufficient maintenance or
maintenance not performed in a timely manner!
There is a risk of collision inside the device due to
insufficient maintenance or maintenance not per-
formed in a timely manner.
– Have maintenance done at the specified inter-
vals.
– Check the rotor for a firm fit before using the
device. Correctly tighten the fastening screw. If
the rotor has play, it must be replaced.
– Check the device for visible damage or defi-
ciencies before use. If you discover deficien-
cies or damage, put the device out of operation
and immediately inform an authorized service
technician.
WARNING!
Warning of premature program abort!
In the event of a premature program abort, such as
a power failure, switching off during the program
run or pulling out the mains plug, the desired effect
on the samples might not be achieved.
– Do not switch off the device during a program
run.
– Do not actuate the emergency release during a
program run.
– Do not pull out the mains plug during a pro-
gram run.
– After a program abort, check whether the sam-
ples can be used.
CAUTION!
Warning of hand injury due to rotor gears!
There is a risk of injury to fingers by the rotor gears
if this is turning. Cuts with possible infections could
result.
–Do not grab between the gears of the rotating
units and the rotor.
– Avoid rotor rotation with the lid open as much
as possible.
– To prevent injuries to third parties, do not leave
the device unsupervised with the lid open.
Danger due to insufficient mainte-
nance or maintenance not per-
formed in a timely manner
Warning of premature program
abort
Warning of hand injury due to rotor
gears
Safety information
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 14

WARNING!
Risk of injury due to parts falling out of the
packaging!
When parts fall out of the transport packaging,
there is a risk of crushing injuries to extremities.
– Observe the warnings for opening the pack-
aging.
– Only open the packaging at the specified point.
WARNING!
Warning of hand injury due to device lid falling
closed unbraked!
If the lid falls closed unbraked, there is a risk of
extremities getting caught.
– Have the device maintained by a service tech-
nician at the specified intervals.
–Always open the device lid completely.
– If a gas pressure spring of the lid is defective,
have it replaced by a service technician.
Risk of injury due to the device
falling out of the packaging
Warning of hand injury due to
device lid falling closed unbraked
Safety information
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 15

WARNING!
Danger posed by selection of unsuitable
parameters for dual centrifugation!
The maximum permissible speed (RPM) for Zen-
triMix applications depends on the sample compo-
sition and sample container. Every ZentriMix
sample container can only be used up to a certain
rotational speed depending on the design and
application; this must not be exceeded under any
circumstances! Otherwise, there is a danger that
the sample container will not withstand the stress,
and the device will be damaged due to the
resulting unbalance; the heat that arises cannot be
compensated by the cooling system, resulting in
the sample container becoming very hot. Only
remove such a container after a sufficient cooling-
off time while wearing appropriate protective
clothing.
– ZentriMix applications are to be carried out
according to the protocol authorized by the
manufacturer.
– Every application not yet described by a manu-
facturer protocol must be checked and vali-
dated for any hazard potential before its use.
Ä Further information on page 41.
– The correct parameter input must always be
checked.
– Do not leave the device unsupervised.
WARNING!
Damage due to incorrect loading for dual cen-
trifugation!
The two rotor receptacles must be loaded strictly
symmetrically with the same weight.
– Observe the loading rules. Ä Chapter 6.5.2
‘Loading a rotor for dual centrifugation’
on page 37!
– Observe the device as it starts up. If unusually
high unbalance occurs, stop the run immedi-
ately by pressing the [Stop/Open] key twice
and check the load. If you cannot determine
that the load is incorrect, put the device out of
operation and inform the Service department.
Danger posed by selection of
unsuitable parameters for dual cen-
trifugation
Damage due to incorrect loading
for dual centrifugation
Safety information
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 16

DANGER!
Danger due to hazardous substances in the
sample
No materials or material mixtures may be pro-
cessed in a way which poses a risk of fire or explo-
sion.
– Therefore, thoroughly check the intended
process regarding this hazard and take the
appropriate measures to avoid personal injury.
–Follow the relevant regulations and directives
for handling chemicals and hazardous sub-
stances.
– In particular, no aggressive chemicals may be
used (e.g. dangerous, corrosive extracting
agents, such as chloroform, strong acids).
WARNING!
Explosion and fire hazard
Do not process any explosive materials or mate-
rials which can react together chemically with high
energy. Substances which could create a haz-
ardous explosive or flammable atmosphere in the
device may not be processed. The estimation of
such risks is the responsibility of the operating
company. The operating company must instruct the
user with regard to this.
– Therefore, thoroughly check the intended
process regarding these hazards and take the
appropriate measures to prevent personal
injury.
– Follow the relevant regulations and directives
for handling chemicals and hazardous sub-
stances.
– In particular, no aggressive chemicals may be
used (e.g. dangerous, corrosive extracting
agents, such as chloroform, strong acids).
Danger due to hazardous sub-
stances in the sample
Explosion and fire hazard
Safety information
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 17

DANGER!
Danger due to the penetration of water or other
liquids!
When water penetrates into the device, there is a
danger of a short circuit and the user suffering an
electric shock.
–Prevent water and moisture from penetrating
into the device.
– Do not pour liquids into the device interior.
– The device must not stand in the rain.
– Transport is only allowed with suitable trans-
port protection.
WARNING!
Warning - Impermissible ambient temperature!
If the ambient temperature is outside of the permis-
sible ambient temperature range for the samples,
these might be compromised.
–Observe the permissible maximum and min-
imum ambient temperatures for setting up the
device.
– Do not place the device next to heat sources or
devices which radiate heat.
– Check the samples after any device over-
heating to see if they can still be used and take
new samples, if necessary.
– Make sure the device is not exposed to direct
sunlight.
– Make sure the device is not exposed to frost.
– Maintain the minimum clearance around the
device.
– Make sure that the fan is not blocked.
– Never operate the device in a continuous run.
WARNING!
Warning - Position changed due to vibration!
The device might fall down if the set-up surface
vibrates or if it is not flat or stable enough.
–Only place the device on a stable, flat surface.
– The set-up surface must reliably bear the
weight of the device.
Danger due to the penetration of
water or other liquids
Warning - Impermissible ambient
temperature
Warning - Position changed due to
vibration
Safety information
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 18

WARNING!
There is a risk of injury if the mechanical emer-
gency release of the device is actuated during
the program run.
If the mechanical emergency release of the lid is
actuated during a program run, the program is
aborted and the rotor runs down unbraked.
– Only actuate the emergency release when the
rotor is stopped.
– Check whether the rotor has stopped before an
emergency release.
Mechanical emergency release
Safety information
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 19

Safety information
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 20