Holbach MBASS30V3 User manual

MBASS30V3
Mik obiologisches Luftp obenahmesystem
M i c o B i o l ogical Ai Sa m pling System
Bedienungsanleitung
Ope ating Manual

Umweltanalytik olbach Gmb
Sperberweg 3, DE-66687 Wadern
Phone: [phone] / 182277
Fax: [phone] / 182278
E-Mail: [email protected]
www.holbach.biz
Hinweis zu Altbatterien
In diesem Gerät befinden sich Batterien:
1 Stück Lithium Knopfzelle Typ CR2032 für Energiepufferung der Echtzeituhr
1 Stück Industriebatterie (Akku) Typ LiPo 14,4 Volt für den Betrieb des Gerätes
Altbatterien (auch Akkus sind Batterien im Sinne des Batteriege-
setzes) dürfen nicht in den ausmüll.
Verbraucher sind verpflichtet, Batterien zu einer geeigneten Sam-
melstelle bei andel oder Kommune zu bringen.
Die Batterien dieses Gerätes können nach Gebrauch auch bei
uns abgegeben werden.
Altbatterien enthalten möglicherweise Schadstoffe oder Schwer-
metalle, die Umwelt und Gesundheit schaden können.
Batterien werden wiederverwertet, sie enthalten wichtige Roh-
stoffe wie Eisen, Zink, Mangan oder Nickel. Das Symbol Mülltonne bedeutet: Batterien und
Akkus dürfen nicht in den ausmüll.
Die Batterien in diesem Gerät enthalten kein Blei, Cadmium oder Quecksilber.
Note on used batteries
This device contains batteries:
1 piece, Type CR2032 lithium button cell for energy buffering of the real-time clock
1 piece, Type LiPo 14.4 Volt industrial battery (rechargeable) for the operation of the device
Used batteries (rechargeable batteries are also batteries in terms
of the Batteries Act) may not be disposed in household waste.
Consumers are obliged to take batteries to an appropriate col-
lection point in the trade or local authority.
The batteries of this device may also be returned to us after use.
Batteries may contain harmful substances or heavy metals that
can harm the environment or personal health.
Batteries are recycled; they contain important raw materials such
as iron, zinc, manganese or nickel.
The rubbish bin symbol stands for: batteries and rechargeable batteries may not be disposed
of in household waste.
The batteries in this device do not contain lead, cadmium or mercury.

Ai Sampling System MBASS30V3
Operating Manual MBASS30V3 LKS100 LKS 30 PS 30 FA 30 1
Contents
Contents
ection Page
Introduction 2
1. The Air Sampling System MBASS30V3 3
2. The Features of MBASS30V3 4
3. Package Contents 5
4. Operating Elements 6
5. Connection Sockets 10
6. Charger 12
7. Operating MBASS30V3 14
8. Configuring Presettings 23
9. Sampling istory 28
10. Configuring MBASS30V3 (Setup) 30
11. The Sampling eads for MBASS30V3 33
12. The Coated Slide 43
13. Information 45
14. Service Functions 46
15. Cleaning and Maintenance Instructions 48
16. afety Instructions 49
17. Technical Data 50
18. Conditions of Warranty 53
19. The MBASSControl Software 54
Appendix A Statistical Correction 56
Appendix B Correction Table for LKS 30 59
Appendix C Correction Table for LKS100 60
Appendix D Troubleshooting 61
Appendix E Declaration of CE Conformity 63
This manual applies to MBASS30V3 with color display and touch operation.

Ai Sampling System MBASS30V3
Operating Manual MBASS30V3 LKS100 LKS 30 PS 30 FA 30
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Introdu tion
Thank you very much for choosing our air sampling system
MBASS30V3.
Please read this manual and the safety instructions carefully before using
the device.
MBASS30V3, including the sampling heads and other accessories,
is a laboratory device and may only be used by trained personnel.
MBASS30V3 may only be used for the purposes described in this
operating manual.
Please note the safety instructions in section 16 of this manual.
The following pictograms are intended to help you reading this operat-
ing manual:
The pictograms used are subject to the Creative Commons License; author of the pic-
tograms is the Regional Computing Centre of Erlangen (RRZE).
We recommended reading this paragraph very carefully.
This paragraph contains more detailed information.
The actions listed in this paragraph are dangerous to per-
sons and the device and are not permitted.
This paragraph describes troubleshooting and maintenance
tasks.

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Operating Manual MBASS30V3 LKS100 LKS 30 PS 30 FA 30 3
1. The Air Sampling System MBASS30V3
The MicroBiological Air ampling ystem MBASS30V3 has been
developed for different sampling methods:
Microbiological air sampling with the sampling
heads LKS100 and LKS 30 for loading of culture
media in 90 mm standard petri dishes for cultivat-
ing.
Particle sampling to detect the total spore con-
centration in the air with the particle sampler
PS 30 for loading adhesively coated microscope
slides.
Filtration on sterile gelatine filters in single-use
units with the filter adapter FA 30 to load round
sterile 80 mm gelatine filters.
Collection of antigenic and allergenic proteins in
the air with the allergen sampler AS100.
Comparison of the methods air sampling and particle sampling:
Air sampling Particle sampling
Analysis after cultivation Microscopic analysis is possible di-
rectly after the sampling
Determination of the cultivable fraction Determination of the total concentration
(cultivable and non-cultivable)
Every cultivable unit results in a visible
unit
Additional information on the environ-
ment (dust particles, fibers etc.)
Determination of the species possible Spore clusters are visible as clusters
Selection by type of culture medium
and conditions of cultivation
Transport time and temperature of
the samples are not critical

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Operating Manual MBASS30V3 LKS100 LKS 30 PS 30 FA 30
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2. The Features of MBASS30V3
Figure 1 MBASS30V3 with sampling head LKS100
10 individual presettings (description text, volume,
volume flow, start delay, logging interval of the sampling
process)
Volume flow can be preset in the range from 30 to 100 l/min
Sampling volume can be preset from 10 liters to 10,000 liters
Start delay from 1 second to 24 hours
4.3 inch (109 mm) color display with additional glass cover
Internal temperature sensor
Connection socket for external temperature/humidity sensor
Internal barometric sensor
Export of the sampling data to a USB memory stick
Robust anodized aluminum profile housing
Integrated battery pack LiPo 14.4 V / 3600 mAh
Quick-charger, usable worldwide
Encapsulated flow of the sampling air to avoid
contamination of the device interior
The features of MBASS30V3

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Operating Manual MBASS30V3 LKS100 LKS 30 PS 30 FA 30 5
3. Pa kage Contents
3. Package Contents
The delivery of the microbiological air sampling system
MBASS30V3 contains the following:
the robust MBASS30V3 base device
quick-charger for operation at 100 - 240 V, 50 - 60 z
this operating manual
a USB connection cable for connection to a PC
the software MBASSControl for configuration, remote control and
adjustment of MBASS30V3 on the included data medium
(USB memory stick)
a replacement 2A slow-blow fuse
And depending on your ordered configuration:
the air sampling head LKS 30 (Volume flow 30 l/min)
the air sampling head LKS100 (Volume flow 100 l/min)
the particle sampling head PS 30 (Volume flow 30 l/min)
the allergen sampling head AS100 (Volume flow 100 l/min)
the filter adapter FA 30 (Volume flow 30 l/min up to 50 l/min)
each as supplementary part (without base unit with hose connector)
temperature/humidity sensor C2A-S by rotronic
the handling case (Tanos systainer T-Loc with PE foam insert)

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Operating Manual MBASS30V3 LKS100 LKS 30 PS 30 FA 30
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4. Operating Elements
Locking springs for
the sampling heads
Carrying handle
4.3 inch color display
with touchpanel
On/Off button
Socket for temper-
ature sensor
Charging socket
Mini-USB socket D
for PC connection
Outlet for the
sampling air
USB socket for
memory stick
Tripod sockets in the device base
Figure 2 MBASS30V3
Figure 3 MBASS30V3 rear view
Release Release
Locking springs for the sampling heads
4. Operating Elements
4.1 Overview of the Operating Elements

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Operating Manual MBASS30V3 LKS100 LKS 30 PS 30 FA 30 7
4. Operating Elements
4.2 Carrying Handle
The handle for comfortable carrying of MBASS30V3 locks into place
every 30 degrees. To adjust the handle, please push both metallic re-
lease buttons (violet arrows in figure 3) at once. Rotate the handle into
the desired position and let go the release buttons. The handle will
lock into place at the next 30 degree step.
4.3 4.3 Inch Color Display with Touchpanel
MBASS30V3 is completely operated using the 4.3 inch color display
with touchpanel (except for the on/off button on the rear). The capac-
itive touchpanel can also be operated with common latex or nitrile
gloves.
4.4 Acoustic ignaler
MBASS30V3 has an acoustic signaler which signals the following
events:
4.5 Locking prings
The three locking springs of MBASS30V3 are made of stainless
spring steel and are to quickly and easily secure the sampling heads
LKS100,AS100, LKS 30, PS 30 and FA 30 to the base. The lock-
ing springs are not intended for carrying MBASS30V3. The shape of
the locking springs is designed such that for proper closing only a light
force is required. If the black locking knob does no longer remain in
the groove on the sampling head, the locking spring can be bent a little
at the first angle, seen from the knob, with flat pliers.
Event Tone Sequence
Power-on 2 short tones
Touch-Click Click sound
Sampling finished 3-tone sequence
Device alarm 2-tone sequence
Battery voltage low 2-tone sequence
Switching off 2 short tones

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Operating Manual MBASS30V3 LKS100 LKS 30 PS 30 FA 30
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4. Operating Elements
4.6 Tripod ockets
There are two tripod sockets in the MBASS30V3 device base:
UNC 1/4 inch (photo) and UNC 3/8 inch (microphone)
Only use tripods that can support the weight of MBASS30V3 (approx.
3 kg) and ensure that they provide a secure standing.
4.7 Media Centering Mechanism
MBASS30V3 is equipped with a fast setting mechanism for centering
the sampling media (petri dishes and PS 30 slide mount).
By turning the setting disk clockwise, the centering pins are moved to-
wards the center (Figure 6). The setting disc snaps into 12 positions.
For the usage of culture media in common standard petri dishes, posi-
tion 7 of the centering mechanism is usually suitable.
Again in short:
Turning clockwise Centering pins narrow
Turning counter-clockwise Centering pins widen
Figure 4 Tripod sockets in the device base
UNC 3/8 inch
UNC 1/4 inch

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Operating Manual MBASS30V3 LKS100 LKS 30 PS 30 FA 30 9
4. Operating Elements
Figure 5 Centering mechanism wide open, disc in position 0
Figure 6 Centering mechanism narrow, disc in position 12
Settings disc
Recessed grips
Centering pin
Position marks
Centering pin Centering pin

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Operating Manual MBASS30V3 LKS100 LKS 30 PS 30 FA 30
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5. Conne tion So kets
5. Connection ockets
In the back of MBASS30V3 there are 4 connection sockets:
5.1 Battery Charger ocket
Connect the charger plug of the delivered quick-charger into the round
battery charger socket (low voltage plug EIAJ5).
Only use the charger that is included in the delivery for charging. Using
any other charger can damage the electronics, the integrated recharge-
able battery and the charger itself.
Polarity: Inner pin is “positive 14.4 volts”, outer wall “ground”.
5.2 Temperature and Humidity ensor ocket
MBASS30V3 features a socket for the temperature and humidity sen-
sor C2A-S by rotronic. The temperature and humidity readings are
read digitally. An optional extension cable can be connected between
the sensor and MBASS30V3. The temperature values are required to
calculate the volume flow. The external temperature/humidity sensor is
automatically detected by MBASS30V3 when connected and its value
gets used for the calculations and the data recording. The advantage
of the external temperature/humidity sensor is the much faster acclima-
tization after a change in temperature compared to the internal flow
sensor.
As the socket contacts are asymmetrical,
the temperature and humidity sensor
C2A-S only fits in one position into the
socket. The sensor is secured with the
sleeve nut.

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Operating Manual MBASS30V3 LKS100 LKS 30 PS 30 FA 30 11
5. Conne tion So kets
5.3 U B ocket H
A FAT formatted memory stick can be connected to the USB socket
(host). Sampling data can be exported in
CSV format from the history view to this
memory stick.
5.4 U B ocket D
MBASS30V3 can be connected to a PC running Microsoft Windows
using the included USB cable (A to Mini)
plugged into the Mini-USB socket D (device).
Figure 7 The temperature/humidity sensor rotronic C2A-S
Figure 8 The USB Socket
Figure 9 The USB socket D

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Operating Manual MBASS30V3 LKS100 LKS 30 PS 30 FA 30
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6. Charger
6. Charger
A quick-charger for charging the integrated Lithium-Polymer battery
pack (14.4 Volt / 3800 mAh) is part of the delivery of MBASS30V3
The charger can be operated at a voltage of 100 - 240 volts and a
mains frequency of 50 - 60 z.
6.1 Using the Charger
Insert the power plug into a mains socket.
After connecting to the mains supply the LED lights up in green.
Insert the plug of the quick-charger into the charger socket of
MBASS30V3.
The state of the charger is indicated with a multi-colored LED:
Orange: Quick-charging until about 80 - 95% charged.
Yellow: Final charge until 100% completed.
Green: The battery is fully charged or the charger plug is not con-
nected.
Fully charging a completely discharged battery can take up to 2.5
hours.
Figure 10 The quick-charger for MBASS30V3
Status indicator

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Operating Manual MBASS30V3 LKS100 LKS 30 PS 30 FA 30 13
6. Charger
Note: The charger can also be used as power supply. The available
current is greater than the current required for the operation of
MBASS30V3.
Please note the safety instructions in section 16!
Figure 11 The rear of the charger contains the status colors,
certification mark and manufacturer logo
Manufacturer logo

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Operating Manual MBASS30V3 LKS100 LKS 30 PS 30 FA 30
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7. Operating MBASS30V3
Operating MBASS30V3 is simple and consists of the following steps:
7. Operating MBASS30V3
Place MBASS30V3 safely Minimum distance of
rear to a wall: 10 cm
Switch on MBASS30V3 Section 7.1
Acclimate with 300l
of sampling air
Only for temperature
differences of > 10 °C
between MBASS30V3
and the air
Prepare the sampling head
LKS: Section 11.1
PS: Section 11.2
FA: Section 11.3
Set up MBASS30V3 See section 8 on how to
modify the presettings
Remove, label and store the
sampling medium
Sections 7.2 and 7.3
Start sampling
maybe prepare and
proceed with another
sampling
Switch off MBASS30V3 Section 7.8
ampling
After sampling
After the last sampling
Prior to sampling

7.1 witching on MBASS30V3
To switch on MBASS30V3, press the on/off button on the back of the
device (Figure 3). When switching on, two short signal tones will sound
and the display shows the personalization text, the firmware version
and the device’s serial number for 5 seconds:
The personalization text (4 lines with max. 25 characters each) can be
defined by the customer prior to shipping and is stored encrypted in
the memory of MBASS30V3.
Afterwards, the main screen with all relevant information is shown:
The external temp./humidity sensor is indicated by the symbol .
The internal temperature measurement is indicated by the symbol .
The symbol “Info” is only shown when an fault has been detected.
The symbol “Log” is shown during sampling if logging of the sam-
pling process is enabled.
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Operating Manual MBASS30V3 LKS100 LKS 30 PS 30 FA 30 15
7. Operating MBASS30V3
Figure 12 Display when switching on
Personalization
Figure 13 Main screen after switching on
Temperature / umidity Air pressure Info Log Batt. Time of Day

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Operating Manual MBASS30V3 LKS100 LKS 30 PS 30 FA 30
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7. Operating MBASS30V3
Each preset program contains:
a description text (editable using the MBASSControl software)
the sampling volume in liters, range: 10 up to 10,000 l
the volume flow in liters per minute, range: 30 up to 100 l/min
the start delay, range: 1 second up to 24 hours
the logging interval in seconds, range: 0 up to 240 s
7.2 electing a Preset Program
Selecting the desired presettings is done by tapping on one of the two
buttons on the left and right of the screen.
If the present preset programs are not as desired, the parameters (except
for the description text) can be modified directly at MBASS30V3.
See section 8 for details on this.
Note: The number of shown preset programs can be limited in the
“Sampling” settings.
Note: The nominal volume flow stated on the sampling heads has to
be respected, since the separation efficiency of the impaction depends
on the volume flow.
Figure 14 Selecting the desired presettings
Selection of the
desired presettings
Selection of the
desired presettings

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Operating Manual MBASS30V3 LKS100 LKS 30 PS 30 FA 30 17
7. Operating MBASS30V3
7.3 tarting the ampling
Prior to sampling, the corresponding sampling head has to be prepared
and the sampling medium is to be inserted. Please note the following
sections describing the sampling heads LKS100 / LKS 30 (Sec-
tion 11.1), PS 30 (Section 11.2) and FA 30 (Section 11.3) for this. The
sampling is started by pressing the Start button.
Note: The air pressure is not shown during sampling.
The sampling number is increased at every start.
Along with the sampling number, the start date (day, month) and the
start time is shown.
The running sampling can be interrupted by tapping the Pa se button.
The running sampling can be aborted before the target volume is
reached by tapping the Stop button.
Figure 15 Starting a sampling
Start sampling
Figure 16 Display during a sampling

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Operating Manual MBASS30V3 LKS100 LKS 30 PS 30 FA 30
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7. Operating MBASS30V3
If a start delay is configured, the sampling starts after this delay. While
this time is running, the remaining delay time is displayed instead of
the remaining time of the sampling.
During the start delay time, the sampling can be started by tapping
the Now button.
During the start delay time, the sampling can be aborted by tapping
the Stop button.
7.4 Pausing the ampling
The running sampling can be interrupted by tapping the Pa se button.
While a sampling is paused, it can be aborted by tapping the Stop
button.
By tapping the Res me button, the pause will be ended and the sampling
will continue.
Figure 17 The start delay is elapsing
Indicator fo the
current delay until
start
Figure 18 Sampling is paused