Tuttnauer LABSCI 11LV ELV User manual

1.1.1
Operation and Maintenance
Manual
Electronic Laboratory Autoclaves
Models LABSCI 11LV
First time published; 06.22.2014
Current revision (G) published 01.17.2016
ELV-D: standard autoclave
C: optional system for fast cooling
PV: optional vacuum pump
BH: optional bio hazard filtration
WR: water recycling system
F: optional fan for super-fast cooling
Cat. No. MAN205-0468003EN Rev G
Heidolph North America , 1235 N Mittel Blvd. Suite B Wood Dale IL 60191
Phone: [phone] Fax: [phone], e-mail: [email protected]

Page 1
Table of Contents
Paragraph page no.
1. GENERAL
1.2 Available optional configurations ...............................................................5
1.3 Incoming Inspection....................................................................................6
1.4 Warranty......................................................................................................6
1.5 Warranty Statement.....................................................................................7
1.6 Accessories...................................................................................................7
2. Safety Instructions................................................................................................8
3. Technical Data....................................................................................................10
3.1 Operating Conditions.................................................................................10
3.2 Directives and Standards...........................................................................10
3.3 Ordering Information................................................................................11
3.4 Accessories.................................................................................................11
3.5 Environment Emission Information .........................................................12
3.6 Electrical Data...........................................................................................12
3.7 Water quality..............................................................................................12
3.8 Specification ..............................................................................................13
3.9 Construction ..............................................................................................14
3.10 Utilities.......................................................................................................14
3.11 Symbol Description....................................................................................15
3.12 Front View.................................................................................................16
1.1. Front View: What is Special for WR Configuration ...................17
3.13 Rear View...................................................................................................18
3.14 Rear View: What is Special for WR Configuration ..................................19
3.15 Overall Dimensions Drawing (mm)...........................................................20
3.16 Overall Dimensions, WR Configuration(mm)...........................................21
3.17 Safety Features..........................................................................................21
3.18 Description of Operation ...........................................................................22
4. Control Panel......................................................................................................24
4.1 Control Panel Drawing..............................................................................24
4.2 Description and Functions of the Front Panel Keyboard.........................25
4.3 Displayed Error Messages / Symbols.........................................................26
4.4 Displayed operational messages / Symbols................................................27
5. Sterilization Programs........................................................................................29
5.1 Program 1: Glass.......................................................................................32
5.2 Program 2: Plastic.....................................................................................33
5.3 Program 3: Liquid A..................................................................................34
5.4 Program 4: Liquid B –Waste....................................................................35
5.5 Program 5: Liquid A –Cooling (C –cooling models only).......................36

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5.6 Program 6: Liquid B –Waste Cooling (C –cooling models only)............37
5.7 Program 7: Bio Hazard 1 (BH, bio-hazard models only) .........................38
5.8 Program 8: Bio Hazard 2 (BH, bio-hazard models only) .........................39
5.9 Program 9: Bio Hazard Liquids (BH, bio-hazard models only) ...............40
5.10 Program 10: Vacuum Test (PV, pre-vacuum models only) ......................42
5.11 Program 11: Warm-Up..............................................................................43
5.12Program 12: Isothermal ............................................................................44
5.13 Program 13: Air Mixture...........................................................................45
5.14 Program 14: Glass Test .............................................................................46
5.15 Program 15: Durham ................................................................................47
6. Checking and Changing Parameters and Other Data .......................................49
6.1 Browsing through the menus ....................................................................49
6.2 Changing a Parameter...............................................................................50
6.3 Quick options screen..................................................................................52
6.4 Logging in and entering the Main menu ..................................................56
6.5 Changing parameters ................................................................................57
6.6 System Parameters.....................................................................................58
6.7 Maintenance ..............................................................................................59
6.8 Cycle parameters........................................................................................60
7. Screens................................................................................................................62
7.1 Screens following a complete successfully cycle –"Cycle Ended" ..........62
7.2 Screens following aborted cycles after complete sterilization stage..........63
7.3 Screens following a fail cycle:...................................................................63
8. Printer.................................................................................................................65
8.1 Printer Output............................................................................................65
8.2 Printer Handling........................................................................................68
9. Preparation before Sterilization .........................................................................71
9.1 Instruments................................................................................................71
9.2 Liquids .......................................................................................................72
9.3 Loading......................................................................................................72
10. Operating Instructions........................................................................................74
10.1 Draining and filling the water reservoir (WR only)..................................74
10.2 Turning on the autoclave ..........................................................................75
10.3 Opening the door .......................................................................................75
10.4 Loading......................................................................................................76
10.5 Closing the door.........................................................................................76
10.6 Starting cycle .............................................................................................76
10.7 Unloading ..................................................................................................76
10.8 Stopping the process and cancelling the ERROR message.......................77
10.9 Start Cycle by Clock mode.........................................................................77
10.10 Moving the Autoclave................................................................................77

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10.11 Loading and Unloading the Device...........................................................78
11. Service and Maintenance ...................................................................................80
11.1 Preventive Maintenance ............................................................................80
11.2 Checking the Safety Valve.........................................................................81
12. Troubleshooting..................................................................................................84
13. Baskets and Containers ......................................................................................89
14. Spare Parts List...................................................................................................89
15. Accessories..........................................................................................................90

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1. Generals
1.1 Introduction
Models LABSCI 11LV ELV/ELVC/ELVPVBH are vertical sterilizers
designed especially for sterilization of instruments, liquids and other
materials in hospital laboratories, laboratories & research institutes,
food laboratories and pharmaceutical facilities.
The sterilizer is fully automatic with a choice of ten programs (including
two custom programs), eliminating any need for operator intervention
during a cycle. The custom programs are programs that may be
changed by an authorized technician. A computerized control unit
enables precise control and monitoring of physical parameters and
clear documentation of the sterilization cycles.
The autoclave is equipped with a safety valve, which blows off at 280
kPa (40 psi) (max. working pressure). The safety valve is located on
the rear of the autoclave. The control system provides adequate
protection, to ensure the safety of personnel and reliable operation with
a minimum of down time.
Printer is an optional addition to the autoclave. The printer prints the
preset and actual parameters of the cycle (temperature, time and
pressure/vacuum).
The autoclave is equipped with an analog pressure gauge designed for
reference only. In case that there is any problem with electricity supply
during a sterilization cycle the pressure gauge will be used to verify that
there is pressure in the chamber.
The temperature and pressure are controlled through sensors placed
inside and outside the media container or bottles.
This manual is intended to give the user a general understanding of
how the autoclave works and indicates best ways to operate and
maintain it in order to obtain optimum results and a trouble free
operation.
After reading this manual, operating the autoclave should be straight
forward. However, since the autoclave is built using high technology
sensitive components, no attempt should be made by the user or any
other unauthorized person to repair or re-calibrate it.

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Only technical personnel having proper qualifications, holding
technical documentation and adequate test instrumentation, are
authorized to undertake repair or service.
1.2 Available optional configurations
ELV is the basic model of the vertical laboratory autoclave.
C
A special feature of the C models is the fast cooling stage for liquids. In
this stage pressure in the chamber is increased by means of
compressed air to compensate the fast decreasing of pressure due to
the fast cooling. The fast cooling shortens the time required for safe
handling of bottles.
Note: There is no cooling option for WR configuration.
PV
Special feature of ELVCPV-D models is the vacuum pump that enables
air removal prior to sterilization and during the drying stage.
The advantages of the pre-vacuum sterilizer in comparison to the
regular gravity displacement steam sterilizer are as follows:
•Removal of air pockets from packs and porous loads and most kinds
of tubes (rubber, plastic etc.) by vacuum at the first stage of the cycle.
•Better steam penetration into the load; resulting in effective
sterilization.
•Better temperature uniformity.
•Better drying of materials with closed doors due to the vacuum
achieved in the chamber at the end of the sterilization cycle.
A special feature of this autoclave is the fast cooling stage for liquids.
In this stage pressure in the chamber is increased by means of
compressed air to compensate the fast decreasing of pressure due to
the fast cooling. The fast cooling shortens the time required for safe
handling of bottles
BH
In ELVCPVBH-D (bio-hazard) models, a bio-hazard filter installed in
the chamber enables sterilizing bio-hazard load without contaminating
the surrounding.
WR
In ELVWR-D (Water recycling) models, water is saved by the water
saving system, because it recycles the water and re-uses it for each
cycle in the following way:

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•Water inlet by the pump from the reservoir and not direct from the
plumbing.
•On slow/fast exhaust, the water gets into the reservoir.
F There is a configuration equipped with fan that allows to shorten the
cooling operation.
1.3 Incoming Inspection
The autoclave should be unpacked and inspected for mechanical
damage upon receipt. Observe packing method and retain packing
materials until the unit has been inspected. Mechanical inspection
involves checking for signs of physical damage such as: scratched
panel surfaces, broken knobs, etc.
If damage is apparent, contact your dealer or point of purchase, so that
they may notify the manufacturer and file a claim with the appropriate
carrier.
All Tuttnauer products are carefully inspected prior to shipment and all
reasonable precautions are taken in preparing them for shipment to
assure safe arrival at their destination.
1.4 Warranty
We certify that this instrument is guaranteed to be free from defects in
material and workmanship for one year against faulty components and
assembly with the exception of glassware, lamps and heaters.
The warranty does not include and does not replace routine
treatment and preventive maintenance to be performed according
to instructions in sec. 12.1 (Preventive Maintenance).
Our obligation is limited to replacing the instrument or parts, after our
examination, if within one year after the date of shipment they prove to
be defective. This warranty does not apply to any instrument that has
been subjected to misuse, neglect, accident or improper installation or
application, nor shall it extend to products that have been repaired or
altered outside the factory without prior authorization from us.
The Autoclave should not be used in a manner not described in
this manual!

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1.5 Warranty Statement
The warranty registration must be completed and returned to our
service departments; within fourteen (14) days of purchase or the
warranty will be void.
Our Technical Service Depts can be reached at:
Heidolph North America, 1235 N Mittel Blvd. Suite B Wood Dale
IL 60191
Service & Repair Division
Troubleshooting, Service incidents, Warranty repair, Repair, Services
and Return authorization
: 224-265-9600 Fax: [phone] e-mail: [email protected]
Tuttnauer USA Co., Ltd., 25 Power Drive Hauppauge, NY 11788,
USA : [phone], [phone], Fax: [phone]
e-mail:[email protected].
Note:
If there is any difficulty with this instrument, and the solution is not
covered in this manual, contact our representative or us first. Do not
attempt to service this instrument yourself. Describe the difficulty as
clearly as possible so we may be able to diagnose the problem and
provide a prompt solution.
If the autoclave is equipped with a printer, send along a copy of the last
printout for our inspection. If replacement parts are needed, stipulate
the model and serial number of the machine.
No products will be accepted for repair without proper authorization
from us. All transportation charges must be paid both ways by the
owner. This warranty will be void if the unit is not purchased from an
authorized full service Tuttnauer dealer.
1.6 Accessories
Basket accessories: A set of two baskets is available for the unit. The
baskets are made of stainless steel wire and have a handle. The
basket allows the operator to load a large quantity of materials into the
chamber.
The pressure scale, printer option and cooling method can be set up at
any time by a technician.
Stainless steel containers: A stainless steel container, for waste
products, is available. This container has vent holes on its upper part

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2. Safety Instructions
The autoclave has unique characteristics. Please read and understand
the operation instructions before first operation of the autoclave. The
following issues may require instructions guidance provided by the
manufacturer: how to operate the autoclave, the door safety
mechanism, the dangers involved in circumventing safety means, how
to ensure that the door is closed, and how to select a correct
sterilization program.
Make sure that you know where the main power switch is, where the
water cut-off valve is and where the compressed air disconnection
valves (if applicable) are located.
Autoclave maintenance is crucial for the correct and efficient function of
the device. We enclose a log booklet that includes maintenance
recommendations, with every device.
The weekly spore test is part of the preventive maintenance plan, along
with the annual validation of the sterilization processes that ensures
appropriate temperature dispersion within the chamber.
Never use the autoclave to sterilize corrosive products, such as: acids,
bases and phenols, volatile compounds or solutions such ethanol,
methanol or chloroform nor radioactive substances.
1. Never start using a new autoclave before the safety, licensing and
authorization department has approved it for use.
2. All autoclave users must receive training in proper usage from an
experienced employee. Every new employee must undergo a training
period under an experienced employee.
3. A written procedure must be established for autoclave operation,
including: daily safety tests, seal inspection and door hinge
inspection, smooth action of the closing mechanism, chamber
cleaning, prevention of clogging and preservation from corrosion,
what is permitted and what is prohibited for sterilization and choosing
a sterilization program.
4. Liquids may be sterilized only with the “liquids” programs. The
container must be covered but not sealed. Sealed bottles may only be
sterilized using a special program. The bottle must be either Pyrex or
a Borosilicate glass bottle. Verify that the two temperature sensors are
located inside two different bottles to assure that the liquid temperature
is 20°C below boiling temperature for sealed bottles at the end of the
cycle.
5. When sterilizing plastic materials, make sure that the item can
withstand sterilization temperature. Plastic that melts in the chamber
is liable to cause a great deal of damage.
6. Individual glass bottles may be placed within an appropriate container
that will be placed in a basket. Never place glass bottles on the floor
of the autoclave. Never fill more than 2/3 of the bottle volume.

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7. On closing the autoclave's door, make sure it is properly locked
before activating.
8. Before withdrawing baskets, wear heat resistant gloves.
9. Before opening the door, verify that there is no pressure in the
chamber (chamber pressure gauge is located on the autoclave's front
panel or door, depends on model).
10. Open the door slowly to allow steam to escape and wait 5 minutes
before you remove the load. When sterilizing liquids, wait 10 minutes.
11. Once a month, ensure that the safety valves are functioning, and
once annually a certified tester must conduct pressure chamber
safety tests.
12. Once annually, or more frequently, effective tests must be performed,
i.e., calibration and validation.
13. Examine the condition of assemblies on a regular basis. Make sure
there are no leaks, breaks, blockages, whistles or strange noises.
14. It is required to conduct maintenance operations as instructed.
15. Immediately notify the person in charge of any deviation or risk for the
proper function of the device.
16. For WR models, drain the water from the mineral free water reservoir
once a week, and refill with fresh mineral-free water or distilled water
(see 10.1).

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3. Technical Data
3.1 Operating Conditions
The autoclave is intended to work in ‘indoor’ conditions only.
Only autoclavable materials shall be used.
The environment shall not exceed an ambient temperature range of
5ºC (41ºF)-40ºC (104ºF) and a relative humidity of 85% respectively.
The operation altitude shall not be over 2000 meters (6561 feet)
(ambient pressure shall not be lower than 80 kPa (11.6 psi)).
The autoclave shall not be used in a manner not specified in this
manual!
Do not use the autoclave in the presence of dangerous gases.
The packed or unpacked device shall be stored in ‘indoor’ conditions.
Operate the autoclave only in the manner specified in the manual. If
the equipment is used in a manner not specified by the manufacturer,
the protection provided by the equipment may be impaired.
Caution!
Waste water should be brought into the public net in accordance
with the local rules or requirements i.e. only non-hazardous
liquids shall be disposed in public sewage!
3.2 Directives and Standards
Every autoclave meets the provisions of the following Directives and is
constructed in compliance with the following Standards:
Tuttnauer. Ltd. company meets the provisions of the following
standards:
ISO 9001:2008 (Quality Systems)
Tuttnauer. Ltd. company also works in conjunction with and refers to:
ANSI/AAMI ST55 American Society of Mechanical Engineers
Section VIII, Division 1, for unfied pressure vessels.
EN 13060 Small Steam Sterilizers.
UL UL 61010-1

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We certify that this instrument is guaranteed to be free from defects in
material and workmanship for one year against faulty components and
assembly with the exception of glassware, lamps and heaters.
The warranty does not include and does not replace routine
treatment and preventive maintenance to be performed according
to instructions in sec. 12.1 (Preventive Maintenance).
Our obligation is limited to replacing the instrument or parts, after our
examination, if within one year after the date of shipment they prove to
be defective. This warranty does not apply to any instrument that has
been subjected to misuse, neglect, accident or improper installation or
application, nor shall it extend to products that have been repaired or
altered outside the factory without prior authorization from us.
The Autoclave should not be used in a manner not described in
this manual!
3.3 Ordering Information
Several items must be specified when ordering the unit from your dealer.
•The chamber diameter and chamber depth required
•Please specify the supply voltage available (i.e. 115v/208v; 1Ph/3Ph)
•The temperature scale needed (Celsius or Fahrenheit).
•The pressure scale needed (kPa or psi)
3.4 Accessories
•Baskets –Different size baskets are available for these units. The
baskets are made of stainless steel wire and have a handle. The
basket allows the operator to load a large quantity of materials into the
chamber.
The baskets are made of stainless steel wire and have a handle. The
basket allows the operator to load a large quantity of materials into the
chamber.
•Stainless steel containers - Different size containers are available for
these units .
The containers are designed for sterilizing waste material. The
containers have vent holes along the upper rim.

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3.5 Environment Emission Information
A. Peak sound level generated by the sterilizer is « 70 / dBA with a back
sound level of 60 dBA.
B. Total heat transmitted by the sterilizer is < 240 W/h
3.6 Electrical Data
3.7 Water quality
Physical Characteristics and Contaminants Levels
The distilled or mineral–free water supplied to the autoclave should have the
physical characteristics and maximum acceptable level of contaminants
indicated in the table below:
Physical Characteristics and Maximum acceptable contaminants levels
in steam for sterilizers (According to EN 13060: 2010)
Element
Condensate –allowable content
Silicon dioxide SiO2
≤0.1 mg/kg
Iron
≤0.1 mg/kg
Cadmium
≤0.005 mg/kg
Lead
≤ 0.05 mg/kg
All other metals except iron,
cadmium, lead
≤0.1 mg/kg
Chloride (Cl)
≤0.1 mg/kg
Phosphate (P2O5)
≤0.1 mg/kg
Conductivity
≤3 μs/cm
pH value (degree of acidity)
5 to 7
Appearance
Colorless clean without sediment
Hardness (Σ Ions of alkaline earth)
≤0.02 mmol/l
Property
Value
Total Power
2400W (2*1200W)
Voltage
230VAC/1Ph
Amperage
10A
Protection against electrical shock
Class I (IEC 60601-1)
Mains supply fluctuation
+/- 10%
Degree of protection by enclosure
IP31

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Compliance with the above data should be tested in accordance
with acknowledged analytical methods, by an authorized
laboratory.
Attention:
We recommend testing the water quality once a month. The use of
water for autoclaves that does not comply with the table above
may have severe impact on the working life of the sterilizer and
can invalidate the manufacturer’s guarantee.
3.7.1 Drain Cooling
The feed water supplied to the drain cooling must meet the following
requirements:
•Hardness: 0.7 - 0.2 mmol/l.
•Water temperature shall not exceed 15°C (59°F).
3.8 Specification
Property
Value
Overall
dimensions
Height
980mm
Width
540mm
Length
450mm
Maximum
dimensions
(door open)
Height
1300mm
Overall
dimensions
(WR
configuration)
Height
1047mm
Width
540mm
Length
434mm
Maximum
dimensions
(door open)
Height
1376
Net weight
101kg.

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Shipping volume
0.56 m3
Shipping weight
115 kg
Shipping
dimensions
length
730mm
width
610mm
height
125mm
Max. Allowable Working
pressure (MAWP)
2.8 bar
Chamber
diameter
280mm
Depth
400mm
Chamber Volume
31L
3.9 Construction
Chamber and door material
Stainless steel
Outer Cabinet
Stainless Steel
Chamber insulation
Fiberglass with reinforced material
3.10 Utilities
Power Supply
Power
1 Ph, 230V/50/60Hz
Recommended
Circuit Breaker
16A
Compressed Air (ELVC only)
1/2" 3 Bar (44-58 psi)
Tap water
1/2", 2-6 Bar (29-44 psi)
Mineral free water
1/2", 2-3 Bar (29-44 psi)
Drain
2" Minimum Withstanding temp. of 80 M
(17676)
Attention!
•A switch or circuit-breaker must be included in the building
installation. This switch or circuit-breaker shall be in close
proximity to the equipment, within easy reach of the operator; and
marked as the disconnecting device for the equipment.
•The electrical net must be protected with a current leakage safety
relay.

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•The electrical network must comply with local rules or
regulations.
•Verify that there is an easy access to the main power switch, to
the water cut-off valve and to the current leakage safety relay.
•Make sure while placing the autoclave, to leave space around the
machine, to give the technician access to service the machine.
•All water connections to autoclave must be performed through
"BACK FLOW PREVENTION SYSTEM" only, as per IEC 61770.
3.11 Symbol Description
Caution! Consult accompanying documents
Caution! Hot surface.
Caution! Hot steam.
Protective earth (Ground)
On-Off

Page 16
3.12 Front View
No.
Description
No.
Description
1
Door handle
9
Printer
2
Door cover
10
N/A
3
USB socket
11
Right service door
4
Display
12
Right service door grip
5
Keyboard
13
Front leg
6
Main switch
14
Front service cover
7
Printer cover
15
Ventilation grill
8
Right service door lock
4
5
15
2
1
3
6
9
10
13
12
14
11
7
8

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1.1. Front View: What is Special for WR Configuration
No.
Description
No.
Description
1
Glass tube indicator of the
reservoir water level
3
Inlet for manual water
reservoir filling
2
Wheel
2
1
2

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3.13 Rear View
No.
Description
No.
Description
1
Fan grill
11
Air pressure regulator (C –cooling models
only)
2
Left service door grill
12
Air pressure gauge (C –cooling models only)
3
Left service door lock
13
Compressed air inlet (C –cooling models
only)
4
RJ45 connector
14
Mineral free water inlet
5
Left service door grip
15
Cavitation Port
6
Cut-off reset button
16
Electrical socket
7
Left service door
17
Circuit breaker
8
Tap water inlet
18
Rear service cover
9
Rear leg
19
Safety valve (under cover)
10
Drain outlet
10
11
12
13
4
5
6
7
8
9
16
15
1
2
3
14
17
18
19

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3.14 Rear View: What is Special for WR Configuration
No.
Description
No.
Description
1
Water reservoir overflow
2
Wheel
3
Water reservoir drain
3
2
1