Invitrogen Safe Imager G6600 Reference guide

The Safe Imager™2.0 Blue-Light Transilluminator (Fig. 1, A) is
designed for viewing stained gels on the laboratory bench top, or
within a gel documentation system. The Safe Imager™2.0 is sup-
plied with a Safe Imager™amber filter unit (Fig. 1, B), a power
cord (Fig. 1, C), and Safe Imager™viewing glasses (Fig. 1, D).
Light from the LED source inside the transilluminator passes
through a blue filter producing a single-intensity signal at
approximately 470 nm (Fig. 2), effective for the excitation of
SYBR®Safe DNA gel stain, and many other other Invitrogen pro-
prietary nucleic acid and protein stains, such as SYBR®Gold,
SYBR®Green I and II, SYPRO®Ruby, SYPRO®Orange, and
Coomassie Fluor™Orange stains. Sensitivity obtained using this
instrument is comparable to that obtained with a standard UV
transilluminator.
Unlike UV-transilluminators, the Safe Imager™2.0 Blue-Light
Transilluminator does not produce UV light and does not require
UV-protective equipment during use.
Safe Imager™2.0 Blue-Light Transilluminator
Introduction
Figure 1. The Safe Imager™2.0 Blue-Light Transilluminator.
25-1054 Rev. Date: 28 August 2009
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Catalog nos. G6600, G6600EU, G6600UK
Corporate Headquarters
5791 Van Allen Way • Carlsbad • CA 92008
Toll Free: 800 955 6288 • F: 760 602 6500
[e-mail]
Contact Information for Other Countries:
See our website www.invitrogen.com
Operating the Safe Imager™2.0 Blue-Light
Transilluminator
1. Ensure that the Safe Imager™2.0 Blue-Light Transilluminator
is placed on a level bench and that there is adequate air
circulation around the unit to prevent overheating.
2. Attach the supplied power cord to the Safe Imager™2.0 blue-
light transilluminator at the back of the device. Plug the other
end of the power cord into a properly grounded electrical out-
let, ensuring the correct plug adaptor is attached (see Safety
Information for additional details).
Note: The small footprint of the Safe Imager™2.0 blue-light
transilluminator may allow it to fit inside the cabinet of your
current gel documentation system.An additional adapter is
supplied to allow direct connection of the power supply unit
to a C14 type cord found in some imaging systems.
Figure 3. On / Off switch: Red mark is seen when on.
3. Place the gel or sample onto the surface of the Safe Imager™
2.0 Blue-Light Transilluminator.
4. Place the amber filter unit on top of the sample or stained gel.
If the gel is larger than the viewing area, rest the amber filter
unit directly on top of the gel, or forgo the amber filter unit
and rely solely on the viewing glasses.
5. Switch the Safe Imager™2.0 transilluminator ON using the
ON/OFF switch located at the front of the instrument (Fig. 3).
DNA stained with blue light-excitable dyes (in solution or in
gel bands) are immediately visible after illumination and the
amber filter unit or viewing glasses are in position.
Important: Before handling your gel or sample, ensure that the
personal safety equipment you are using is appropriate for the
hazards posed by the chemicals that may be present. Make sure
to use either the Safe Imager™amber filter unit or Safe Imager™
viewing glasses; to prevent prolonged exposure of your eyes to
the intense blue light (see Safety Information for more details).
B
A
C
D
Figure 2. Emission spectrum for the Safe Imager™2.0 Blue-Light
Transilluminator.
6. Any standard imaging device may be used to document
results. Satisfactory results are often obtained by placing the
amber filter unit on top of the gel and photographing/
imaging as normal.
Note: The Safe Imager™2.0 Blue-Light Transilluminator has a
very slim design compared to a UV transilluminator, so the
distance between the camera and gel may require adjustment.
Also, some CCD documentation systems may include a filter
that will work in place of the amber filter unit (contact the
manufacturer for filter specifications).
7. Use the viewing glasses when excising bands from DNA gels.
The viewing glasses allow the bands to be visualized while
leaving the gel surface unobstructed.
Note: Blue-light transillumination is ideal for cloning, and
results in dramatically increased cloning efficiencies compared
to UV transillumination (Fig. 4).
8. Switch the Safe Imager™2.0 Blue-Light Transilluminator off
after use.
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Figure 4. Gels loaded with equal amounts of a PCR product (1.25 kb
gene fragment from Ultimate™ORF IOH #11050) were stained with
either SYBR®Safe DNA gel stain (1:10,000 in TBE) or ethidium bromide
(0.5 µg/ml in TBE) following electrophoresis. The gel stained with
SYBR®Safe stain was visualized on a blue-light box with light emission
identical to that produced by the Safe Imager™2.0 blue-light trans-
illuminator. The ethidium bromide-stained gel was visualized using UV
transillumination. Bands were excised at defined exposure times. DNA
was purified from the gel fragments under identical conditions and used
in parallel sub-cloning reactions. Following transformation into One
Shot®TOP 10 chemically competent bacteria, three serial dilutions were
plated and colonies counted using an Alpha Innotech imaging system.
Product List
The Safe Imager™Blue-Light Transilluminator can be used for the visualization of E-Gel®Products containing blue light-excitable stains:
Cat. no. Product Name Unit Size
S37103 Safe Imager™viewing glasses each
S33102 SYBR®Safe DNA Gel Stain, 10,000X concentrate 400 µl
The Safe Imager™Blue-Light Transilluminator can be used for the visualization of E-Gel®Products containing blue light-excitable stains:
G4010-01 E-Gel®EX Gel, 1% 10 gels
G4010-02 E-Gel®EX Gel, 2% 10 gels
G6610-02 E-Gel®SizeSelect™2% Agarose Gels 10 gels
G5218-01 E-Gel®1.2% with SYBR®Safe 18 gels
G5218-02 E-Gel®2% with SYBR®Safe 18 gels
G7208-02 E-Gel®96 2% with SYBR®Safe 8 gels
G6618-08 E-Gel®CloneWell™0.8% SYBR®Safe Gels 18 gels
This products is covered by Limited Use Label License No. 5 and No. 324. For details on the license, see www.invitrogen.com.
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
Copyright 2009, Invitrogen Corporation. All rights reserved. This information is subject to change without notice. Product of Israel.
Corporate Headquarters
5791 Van Allen Way • Carlsbad • CA 92008
Toll Free: 800 955 6288 • F: 760 602 6500
[e-mail]
Contact Information for Other Countries:
See our website www.invitrogen.com
Leaving the Safe Imager™2.0 switched ON for an extended time,
particularly in a non-ventilated environment, can lead to internal
overheating, and activation of an automatic temperature control
circuit, that will shut off the illumination.
The illumination is restored automatically after the unit has
cooled by ~10° C. If the cooling process has begun, switching the
unit OFF and then ON can shorten the time needed to restore
illumination.
Cleaning Instructions
•Viewing surface dimensions: 190 × 190 mm (7.5 × 7.5 in)
•Overall dimensions: 295 × 325 × 65 mm (11.6 × 12.8 × 2.6 in)
•LED life: 50,000 hours
•Included accessories: amber filter unit and viewing glasses
•The Safe Imager™2.0 Blue-Light Transilluminator complies
with the European Community Safety requirements.
•This device contains Class 1 LED products.
Instrument Specifications
Important: Disconnect the Safe Imager™2.0 Blue-Light Trans-
illuminator from the electrical outlet before cleaning.
Clean the device with a dry cloth, or with water and mild soap.
Ethanol may also be used. Avoid damaging or scratching the
glass surface of the Safe Imager™2.0 Blue-Light Transilluminator
with abrasive cleaners, sharp instruments, or harsh solvents.
Safety Information
The Safe Imager™2.0 Blue-Light Transilluminator is an electrical
device.
•Never touch the power cord or outlet with wet hands
•Do not use this device in damp areas or while standing on
damp floors
•Do not use the device, if a crack is apparent in Safe Imager™
2.0 illumination or diffuser surface
•Do not attempt to open the Safe Imager™2.0 blue-light trans-
illuminator
The Safe Imager™2.0 Blue-Light Transilluminator is supplied
with a power cord suitable for your region. This power cord
has a universal transformer compatible with 110 V to 220 V.
Only the power cord supplied with the Safe Imager™Blue-
Light Transilluminator should be used to power the device.
Always disconnect the Safe Imager™2.0 Blue-Light Trans-
illuminator from the electrical outlet before cleaning the device.
The Safe Imager™2.0 blue-light transilluminator does not pro-
duce UV-light. However, the intense blue light emitted by the
Safe Imager™may promote macular degeneration upon pro-
longed exposure, especially in those prone to such problems
(e.g. people with fair complexion and blue eyes, nutritional or
endocrine defects, or those who are aging). Use the Safe Imager™
amber filter unit or Safe Imager™viewing glasses provided with
this device to protect your eyes. The amber filter unit and view-
ing glasses are for viewing stained gels using the Safe Imager™
2.0 Blue-Light Transilluminator.
Note: The amber filter unit and viewing glasses are NOT
designed as protection for UV emission, and will NOT protect
your eyes when viewing gels on UV transilluminators.
Do not leave the Safe Imager™2.0 Blue-Light Transilluminator
switched on for extended periods of time. After viewing and
documenting the gel or sample, always switch the unit off.
Refer to the E-Gel®Technical Guide for explanation of symbols
and, warnings including WEEE and caution labels.
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