Invitrogen E-Gel Power Snap Plus User manual

For Research Use Only. Not for use in diagnostic procedures.
E‑Gel™Power Snap Plus Electrophoresis
System
USER GUIDE
For use with E‑Gel™, E‑Gel™EX, E‑Gel™48, and E‑Gel™96
Agarose Gels, and E-PAGE™Gels
Catalog Numbers G9100, G9200, G9101, G9201, G9301, G9341, G9342, G9391, G9392
Publication Number MAN0002591
Revision A.0

Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256
For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition.
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE
LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR
ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Revision history: Pub. No.MAN0002591
Revision Date Description
A.0 5 April 2022 New user guide for the E‑Gel™Power Snap Plus Electrophoresis System.
Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these
products, you accept the terms and conditions of all applicable Limited Use Label Licenses.
TRADEMARKS: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
©2022 Thermo Fisher Scientific Inc. All rights reserved.

Contents
■CHAPTER1Productinformation .................................................. 7
Productdescription ............................................................. 7
Product contents ................................................................ 7
Base module kit contents .................................................... 7
Camera module kit contents ................................................. 8
Systemcomponents ............................................................. 8
Parts of the E‑Gel™Power Snap Plus Base Module and AC/DC power adapter ..... 8
Parts of the E‑Gel™Power Snap Plus cameramodule ........................... 9
Rear view of the E‑Gel™Power Snap Plus camera module and base module ....... 9
Touchscreen controls ...................................................... 10
Required materials not provided ................................................. 12
First time instrumentsetup ...................................................... 12
Set up the basemodule .................................................... 12
Set up the cameramodule .................................................. 12
■CHAPTER2Methods ............................................................. 24
Using the E‑Gel™Power Snap Plus ElectrophoresisDevice .......................... 24
User interface overview ..................................................... 24
Required materials ......................................................... 25
Preparesamples ........................................................... 25
Dilute samples containing highsalt .......................................... 26
DNA ladder preparationguidelines ........................................... 26
Prepare thegel ............................................................ 27
Sample loadingguidelines .................................................. 27
Loadsamples ............................................................. 28
Run thegel ............................................................... 28
Checkstatus .............................................................. 29
Modify arun .............................................................. 30
Using the E‑Gel™Power Snap Plus Camera ....................................... 31
User interface overview ..................................................... 31
Attach the camera ......................................................... 32
Remove the camera ........................................................ 32
Viewgel .................................................................. 32
Captureimage ............................................................ 32
E‑Gel™Power Snap Plus Electrophoresis System User Guide 3

View image gallery ......................................................... 33
Exportimage .............................................................. 36
Maintenance .................................................................. 38
Cleaning and maintenance .................................................. 38
Materials required .......................................................... 38
Clean the E‑Gel™Power Snap Plus ElectrophoresisDevice ..................... 38
Replace the camera battery ................................................. 39
Upgrade the systemfirmware ............................................... 39
Self Verification test ........................................................ 42
Restore factory settings (Administrator profile only)............................ 42
■APPENDIXATroubleshooting .................................................... 43
■APPENDIXBRecommended instrumentsettings .............................. 45
AboutInstrument .............................................................. 45
Recommended instrumentsettings .............................................. 45
Instrumentsettings ........................................................ 46
Maintenance & services ......................................................... 50
■APPENDIXCE‑Gel™agarosegels ............................................... 51
Nucleic acid stains used in E‑Gel™agarosegels ................................... 51
SYBR™Safe DNA gelstain .................................................. 51
SYBR™gold II gelstain ..................................................... 52
Opening E‑Gel™cassettes ...................................................... 52
Gelknife .................................................................. 52
Open E‑Gel™EX and NGS cassettes with a gel knife ........................... 53
Cleaning and storage ....................................................... 54
E‑Gel™agarose gel disposalguidelines ........................................... 54
■APPENDIXDE‑Gel™CloneWell™II AgaroseGels .............................. 55
Generalguidelines ............................................................. 55
Preparesamples ............................................................... 55
Prepare thegel ................................................................ 56
Loadsamples ................................................................. 56
Run thegel .................................................................... 57
Checkstatus .................................................................. 57
Preparewells .................................................................. 58
Collect DNA fragment .......................................................... 58
Guidelines for estimating runtime ................................................ 59
Contents
4E‑Gel™Power Snap Plus Electrophoresis System User Guide

■APPENDIXEE‑Gel™SizeSelect™II AgaroseGels .............................. 60
Generalguidelines ............................................................. 60
Preparesamples ............................................................... 60
Prepare thegel ................................................................ 61
Loadsamples ................................................................. 61
Run thegel .................................................................... 62
Checkstatus .................................................................. 62
Preparewells .................................................................. 63
Collect DNA fragment .......................................................... 63
Guidelines for estimating runtime ................................................ 65
Quantitation of isolatedDNA .................................................... 67
■APPENDIXFSpecifications ...................................................... 68
Systemspecifications .......................................................... 68
■APPENDIXGRelated products .................................................. 70
Accessory products ............................................................ 70
Choosing the rightgel ...................................................... 70
E‑Gel™AgaroseGels ....................................................... 72
Choosing the right DNAladder .............................................. 74
Ladders andbuers ........................................................ 75
Accessory items ........................................................... 75
■APPENDIXHSafety ............................................................... 76
Before starting ................................................................. 76
Installing theinstrument ......................................................... 77
Explanation of symbols andwarnings ............................................ 77
Symboles d’information ........................................................ 78
Environmental requirements ..................................................... 80
Electromagnetic compatibility (EMC) standards .................................... 81
Class Anotice ............................................................. 81
Electricalsafety ................................................................ 81
Service operation requirements .............................................. 81
LED (Light-Emittingdiode) ...................................................... 82
Chemicalsafety ................................................................ 83
Biological hazardsafety ......................................................... 84
Contents
E‑Gel™Power Snap Plus Electrophoresis System User Guide 5

■APPENDIXIDocumentation and support ....................................... 85
Customer and technical support ................................................. 85
Limited product warranty ........................................................ 85
Contents
6E‑Gel™Power Snap Plus Electrophoresis System User Guide

Product information
Product description
The Invitrogen™E‑Gel™Power Snap Plus Electrophoresis System is an easy-to-use system designed for
performing electrophoresis and documentation of nucleic acids with pre-cast E‑Gel™agarose gels and
E-PAGE™gels.
The system consists of a base module containing a power supply, blue light transilluminator, and amber
filter in one unit, and a camera module capable of high-resolution image capture and documentation
without the need for focusing or adjustment.
The system is compatible with precast E‑Gel™, E‑Gel™EX, E‑Gel™48, and E‑Gel™96 agarose gels, as
well as E-PAGE™gels.
Product contents
Base module kit contents
The contents of the E‑Gel™Power Snap Plus Electrophoresis Device kit are listed in the following table.
The device is shipped at room temperature.
See “System components” on page8 for specifications and description of the E‑Gel™Power Snap
Plus Electrophoresis Device, and “First time instrument setup” on page12 to set up the instrument.
Product Quantity
E‑Gel™Power Snap Plus Electrophoresis Device 1
E‑Gel™Adapter for E‑Gel™Power Snap Plus Electrophoresis Device 1
Power cord with AC/DC power adapter (for US/Canada/Taiwan/Japan, Europe,
or UK)
1
Safe Imager™Viewing Glasses 1
1
E‑Gel™Power Snap Plus Electrophoresis System User Guide 7

Camera module kit contents
The contents of the E‑Gel™Power Snap Plus Camera kit are listed in the following table. The camera is
shipped at room temperature.
See “System components” on page8 for specifications and description of the E‑Gel™Power Snap
Plus Camera, and “First time instrument setup” on page12 to set up the instrument.
Product Quantity
E‑Gel™Power Snap Plus Camera 1
System components
Parts of the E‑Gel™Power Snap Plus Base Module and AC/DC power
adapter
1
2
4
3
5
6
7
7
8 9
1011
1E‑Gel™cassette compartment
2Blue-light transilluminator
3Open button for filter lid
4Electrophoresis unit control touch screen
5Lid with amber filter (open)
6Docking connector
7Electrodes
8Adaptor
9DC output cable
10 Connector to DC input of electrophoresis unit
11 AC power cord inlet
Chapter1Product information
System components
1
8E‑Gel™Power Snap Plus Electrophoresis System User Guide

Parts of the E‑Gel™Power Snap Plus camera module
1
2
3
4
1Touch screen
2USB port for image export/camera module firmware upgrade
3Handhold for lifting (on both sides)
4Battery compartment
Rear view of the E‑Gel™Power Snap Plus camera module and base module
6
3
4
2
6
5
1
1USB port for wireless module
2Ethernet port
3DC input port
4Power switch
5USB port for image export/base module firmware upgrade
6Ventilation ports
Chapter1Product information
System components 1
E‑Gel™Power Snap Plus Electrophoresis System User Guide 9

Touchscreen controls
Table1General touchscreen controls
Button Function
Returns to the previous screen
Go to Home screen
Go to Sign in screen
Go to Settings screen
Close the current modal window.
Touchscreen status indicators
See page24 for an overview of the base module, and page31 for an overview of the camera module.
Table2Status indicators
Button Function
1
4
View instrument status
1. Time remaining
2. Protocol status (step, paused, etc.)
Indicates whether a USB device is inserted into the
instrument.
Indicates whether the Wi-Fi is on or o.
Indicates whether the instrument is connected to wired
network.
Indicates whether the instrument is connected to the
Thermo Fisher™Connect Platform.
Chapter1Product information
System components
1
10 E‑Gel™Power Snap Plus Electrophoresis System User Guide