Invitrogen E-Gel Power Snap Plus User manual

For Research Use Only. Not for use in diagnostic procedures.
E‑Gel™Power Snap Plus Electrophoresis
System
USER GUIDE
For use with E‑Gel™, E‑Gel™EX, E‑Gel™48, and E‑Gel™96
Agarose Gels, and E-PAGE™Gels
Catalog Numbers G9100, G9200, G9101, G9201, G9301, G9341, G9342, G9391, G9392
Publication Number MAN0002591
Revision A.0

Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256
For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition.
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE
LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR
ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Revision history: Pub. No.MAN0002591
Revision Date Description
A.0 5 April 2022 New user guide for the E‑Gel™Power Snap Plus Electrophoresis System.
Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these
products, you accept the terms and conditions of all applicable Limited Use Label Licenses.
TRADEMARKS: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
©2022 Thermo Fisher Scientific Inc. All rights reserved.

Contents
■CHAPTER1Productinformation .................................................. 7
Productdescription ............................................................. 7
Product contents ................................................................ 7
Base module kit contents .................................................... 7
Camera module kit contents ................................................. 8
Systemcomponents ............................................................. 8
Parts of the E‑Gel™Power Snap Plus Base Module and AC/DC power adapter ..... 8
Parts of the E‑Gel™Power Snap Plus cameramodule ........................... 9
Rear view of the E‑Gel™Power Snap Plus camera module and base module ....... 9
Touchscreen controls ...................................................... 10
Required materials not provided ................................................. 12
First time instrumentsetup ...................................................... 12
Set up the basemodule .................................................... 12
Set up the cameramodule .................................................. 12
■CHAPTER2Methods ............................................................. 24
Using the E‑Gel™Power Snap Plus ElectrophoresisDevice .......................... 24
User interface overview ..................................................... 24
Required materials ......................................................... 25
Preparesamples ........................................................... 25
Dilute samples containing highsalt .......................................... 26
DNA ladder preparationguidelines ........................................... 26
Prepare thegel ............................................................ 27
Sample loadingguidelines .................................................. 27
Loadsamples ............................................................. 28
Run thegel ............................................................... 28
Checkstatus .............................................................. 29
Modify arun .............................................................. 30
Using the E‑Gel™Power Snap Plus Camera ....................................... 31
User interface overview ..................................................... 31
Attach the camera ......................................................... 32
Remove the camera ........................................................ 32
Viewgel .................................................................. 32
Captureimage ............................................................ 32
E‑Gel™Power Snap Plus Electrophoresis System User Guide 3

View image gallery ......................................................... 33
Exportimage .............................................................. 36
Maintenance .................................................................. 38
Cleaning and maintenance .................................................. 38
Materials required .......................................................... 38
Clean the E‑Gel™Power Snap Plus ElectrophoresisDevice ..................... 38
Replace the camera battery ................................................. 39
Upgrade the systemfirmware ............................................... 39
Self Verification test ........................................................ 42
Restore factory settings (Administrator profile only)............................ 42
■APPENDIXATroubleshooting .................................................... 43
■APPENDIXBRecommended instrumentsettings .............................. 45
AboutInstrument .............................................................. 45
Recommended instrumentsettings .............................................. 45
Instrumentsettings ........................................................ 46
Maintenance & services ......................................................... 50
■APPENDIXCE‑Gel™agarosegels ............................................... 51
Nucleic acid stains used in E‑Gel™agarosegels ................................... 51
SYBR™Safe DNA gelstain .................................................. 51
SYBR™gold II gelstain ..................................................... 52
Opening E‑Gel™cassettes ...................................................... 52
Gelknife .................................................................. 52
Open E‑Gel™EX and NGS cassettes with a gel knife ........................... 53
Cleaning and storage ....................................................... 54
E‑Gel™agarose gel disposalguidelines ........................................... 54
■APPENDIXDE‑Gel™CloneWell™II AgaroseGels .............................. 55
Generalguidelines ............................................................. 55
Preparesamples ............................................................... 55
Prepare thegel ................................................................ 56
Loadsamples ................................................................. 56
Run thegel .................................................................... 57
Checkstatus .................................................................. 57
Preparewells .................................................................. 58
Collect DNA fragment .......................................................... 58
Guidelines for estimating runtime ................................................ 59
Contents
4E‑Gel™Power Snap Plus Electrophoresis System User Guide

■APPENDIXEE‑Gel™SizeSelect™II AgaroseGels .............................. 60
Generalguidelines ............................................................. 60
Preparesamples ............................................................... 60
Prepare thegel ................................................................ 61
Loadsamples ................................................................. 61
Run thegel .................................................................... 62
Checkstatus .................................................................. 62
Preparewells .................................................................. 63
Collect DNA fragment .......................................................... 63
Guidelines for estimating runtime ................................................ 65
Quantitation of isolatedDNA .................................................... 67
■APPENDIXFSpecifications ...................................................... 68
Systemspecifications .......................................................... 68
■APPENDIXGRelated products .................................................. 70
Accessory products ............................................................ 70
Choosing the rightgel ...................................................... 70
E‑Gel™AgaroseGels ....................................................... 72
Choosing the right DNAladder .............................................. 74
Ladders andbuers ........................................................ 75
Accessory items ........................................................... 75
■APPENDIXHSafety ............................................................... 76
Before starting ................................................................. 76
Installing theinstrument ......................................................... 77
Explanation of symbols andwarnings ............................................ 77
Symboles d’information ........................................................ 78
Environmental requirements ..................................................... 80
Electromagnetic compatibility (EMC) standards .................................... 81
Class Anotice ............................................................. 81
Electricalsafety ................................................................ 81
Service operation requirements .............................................. 81
LED (Light-Emittingdiode) ...................................................... 82
Chemicalsafety ................................................................ 83
Biological hazardsafety ......................................................... 84
Contents
E‑Gel™Power Snap Plus Electrophoresis System User Guide 5