Kent Scientific SomnoFlo User manual

SomnoFlo®
Low-Flow Electronic
Anesthesia System
User’s Guide

©2021 Kent Scientific Corporation, Documentation Version 1.2 for Firmware Revision 1.2.0 September 2021
SomnoFlo®
Low-Flow Electronic
Anesthesia System
User’s Guide

TABLE OF CONTENTS
Introduction
System Components ....................................................................1
Navigating SomnoFlo ..................................................................1
SomnoFlo®
Small Animal Anesthesia................................................................... 3
Set Up Hardware .......................................................................... 3
Configure Settings....................................................................... 7
Delivering Anesthesia ................................................................ 8
Recommended Flow Rates........................................................9
Troubleshooting ......................................................................... 10
General Information
Contact ......................................................................................... 11
Warranty ...................................................................................... 11
Satisfaction Guarantee ..............................................................12
Specifications ..............................................................................12
Index....................................................................................................13

1
Introduction
The SomnoFlo is not designed, intended or authorized for use in human applications.
System Components

2
Touch the Menu button for anesthetic delivery settings.
Run Screen
Menu
There are two Dials, Air/O2 Flow and %Anesthetic, which can be used on the Run
Screen and in the Menu options to control their respective parameters.
Navigating SomnoFlo®
SomnoFlo Touch Screens oer quick access to menus and settings.

3
SomnoFlo®Small Animal Anesthesia
Set Up Hardware
1) Connect the INSP portion of the 2-Accessory Connector to the port labeled “Out.”
2) Connect the INSP nose cone tubing to one branch, and the INSP chamber tubing to the
other branch.
Note: The nose cone adapter has a specific inlet with tubing labeled INSP.
3) Connect the EXP portion of the 2-Accessory Connector to the EXP tubing on the
chamber and nose cone.
4) Attach the Charcoal Canister with the Canister Tubing Assembly.
5) If using Room Air as a carrier gas, leave the Ambient Air port open to atmosphere.
If using Compressed Gas, use the Compressed Gas Assembly to connect a pressure-
regulated gas source to the SomnoFlo’s Compressed Gas port.
Note: The SomnoFlo requires an input pressure of 10-15 PSI. If your gas
source has a higher output, use the Pressure Reducer to automatically
regulate gas sources of up to 100 PSI to the required 10-15 PSI range.
6) Connect the bottle top adapter to a bottle of anesthetic.
Note: If using a 100mL bottle, the inner bottle top adapter tubing can be
cut to a shorter length.
7) Connect the anesthetic delivery tubing directly to the bottle top adapter. Place the
isoflurane bottle into the SomnoFlo’s integrated holder, taking care not to kink the
delivery tubing.
8) Turn on the SomnoFlo to begin automatic priming. If the SomnoFlo is on during a bottle
change, touch Anesthetic Control > Prime.

4
The SomnoFlo will automatically prime when powered on. Priming only needs to be
performed before the first use of the day and after changing the anesthetic bottle.
The Prime cycle requires approximately 70 seconds to complete. The SomnoFlo will
automatically perform a Purge at the end of each Prime cycle.
9) Important: Run the system for several minutes prior to introducing any animals. This
warm-up period is important to equilibrate to current settings after priming.
During warm up, set the gas/air flow rate to 200 ml/min and the anesthetic to 2%. Allow
the SomnoFlo to run at these settings for about 5 minutes to reach a stable anesthetic
gas flow. This will deliver the proper amount of anesthetic into the vaporizer block at a
median gas flow and allow the vaporizer block time to warm up.
Note: While warming up, the SomnoFlo should be connected to a charcoal
canister for scavenging.
10) Once the warm up is complete, perform a Purge to clear the induction chamber of any
excess anesthetic. The SomnoFlo is now ready to use.
Setup Examples
SomnoFlo with 2-Accessory Connector for Chamber and Nose Cone

5
SomnoFlo with Optional 3-Accessory Connector for Chamber and 2 Nose Cones
SomnoFlo with 2-Accessory Connector for Chamber and Ventilator

6
SomnoFlo with 2-Accessory Connector for Chamber and Stereotaxic Gas Anesthesia Mask

7
Configure Settings
Touch Menu to enter settings:
1) PreSet options
o Low Flow can be used for nose cone settings. Enter desired flow rate and
anesthetic concentration for maintenance. These values will be stored for quick
access by pressing the Low Flow button on the main screen.
o High Flow can be used for induction settings. Enter desired flow rate and
anesthetic concentration for induction. These values will be stored for quick
access by pressing the High Flow button on the main screen.
o Purge can be used to reduce residual anesthetic in the chamber prior to
opening. These values will be stored for quick access by pressing the Purge
button on the main screen.
Note: “Purge” pushes air/O2 through the vaporizer without the addition
of anesthetic. This is an eort to reduce the levels of residual anesthetic
in the vaporizer, induction chamber and other attachments connected to
the outlet of the SomnoFlo. Note that because of the complexity of the
attachments, and their dead space volume and flow paths, anesthetic
levels may not be lowered to acceptable levels or even reduced using
Purge.
To more completely reduce anesthetic levels in the attachments, an
external source of compressed gas or air should be connected to the
system with external controls.
2) Carrier Gas Source Air/Compressed gas (O2)
a. Touch the Air/Oxygen button to select a carrier gas. Select “Air” to utilize the
SomnoFlo’s internal air pump, or select “Oxygen” to use a compressed gas
source.
3) Anesthetic Control
a. Prime: Fills the SomnoFlo tubing with liquid anesthetic from the bottle. See
“Filling the SomnoFlo”, above.
b. Empty: Removes liquid anesthetic from the internal components of the
SomnoFlo and empty liquid back into the bottle.
Note: After emptying the SomnoFlo, use Prime to re-enable anesthetic delivery.

8
c. Select Anesthetic Type: Choose Isoflurane or Sevoflurane.
d. Set Used Anest to Zero: Reset how much anesthetic has been used. Select when
replacing the isoflurane bottle.
e. Total Delivered Alarm: Sound an alarm when a certain volume of anesthetic has
been used. Set as a reminder to replace the isoflurane bottle.
f. Monitor Delivery Beep: Sound a beep at volume delivered intervals while the
SomnoFlo is in use.
Delivering Anesthesia
1. When powered on, the SomnoFlo will automatically begin a prime cycle. Once
completed, allow the system to warm up for 5 minutes at 200mL/min and 2% isoflurane
before moving to step 2.
2. Enter any required settings, include the Air/Oxygen, Anesthetic Type, Preset Flow Rates,
and Alarms.
3. Adjust clips to send airflow to the induction chamber.
4. Place animal in induction chamber and touch “High Flow”.
a. The SomnoFlo will automatically deliver the flow and anesthetic concentration set in
the Presets menu. If you need to make adjustments, use the dials to change the flow
and anesthetic concentration.
5. When the animal is fully anesthetized, touch “High Flow” again to stop delivery.
6. If desired, purge the chamber using “Purge”.
7. Remove the animal from the chamber and position on the nose cone.
8. Adjust clips to direct airflow to the mask.
9. Touch “Low Flow” to resume anesthetic delivery at preset flow and anesthetic
concentration.
a. The SomnoFlo will automatically deliver the flow and anesthetic concentration set in
the Presets menu. If you need to make adjustments, use the dials to change the flow
and anesthetic concentration.
10. When finished with the procedure, touch “Low Flow” again to stop delivery and recover
the animal.
11. Touch Menu > Anesthetic Control > Empty to empty the SomnoFlo of anesthetic, then
remove the anesthetic bottle from the system.

9
Caution: Be sure to perform the Empty cycle before powering o the system or
disconnecting the anesthetic bottle.
When the system is not in use, remove the bottle top adapter and replace the
manufacturers cap onto the anesthetic bottle.
Recommended Flow Rates
9/21 V4.0
Electronic Vaporizer recommended Flow Rates
Body Weight Low-Profile Nose Cone
(mL/min)
Standard Face Mask
(mL/min)
Stereotaxic Mask
(mL/min)
<60g
100 100-120 120-140
60-80g
100
100-150
140-180
80g-100g
100
120-180
180-210
100-120g
100-120
140-200
200-240
120-140g
120-130
150-220
240-270
140-160g
130-150
180-250
270-300
160-180g
150-160
200-270
300-330
180g-200g
160-180
220-300
330-350
200-300g
180-250
230-400
350-480
300g-400g
250-300
320-500
480-580
400-500g
300-360
400-600
600-720
500-600g
360-420
480-680
720-830
600-700g
420-460
550-770
830-920
700-800g
460-520
620-850
920-1000
800-900g
520-560
680-740
1000, with caution
900-1000g
560-610
740-1000
, with caution
1000
, with caution
1000-1100g
610-660 820-1000, with caution Not Recommended
1100-1250g
660-730
880-1000
, with caution
Not Recommended
Refer to your User’s Manual for complete instructions.

10
Problem Possible Cause Solutions
SomnoFlo® Troubleshooting
• Increase the flow rate by turning the
Air/O2 Dial.
• Verify that tubing is connected properly,
unobstructed, and correct clips are open.
Anesthetic depth too
light, or the animal
wakes up
Anesthetic depth too
deep
Minute volume displays
0mL/min.
• Flow rate is too low
• Incorrect tubing setup
• Flow rate is too low
• Incorrect Air Source setting
• Blockage at air inlet or exhaust
• Compressed gas source not
turned on or turned on at
incorrect pressure
• Airflow not turned on
• Increase the flow rate by turning the
Air/O2 Dial.
• Check that Air Source is set properly by
touching Menu and Air/Oxygen.
• Check all connections and ports for
blockages.
• If using compressed gas, be sure the gas
source is turned on and flowing properly.
• Pressure should be between 10 and 15 PSI.
• Check that airflow is turned on, indicated
by the shading of the Low Flow, High
Flow, or Purge buttons on the Run screen.

11
General Information
Thank you for purchasing a SomnoFlo. We truly appreciate your business. We strongly
advise that you read and study this User’s Guide to fully appreciate all the features, benefits,
and capabilities of the SomnoFlo.
Contact Information
Kent Scientific Corporation Toll-Free: 888-5RATTUS (888-572-8887)
1116 Litchfield Street Outside US: 860-626-1172
Torrington, Connecticut 06790 Fax: [phone]
E-mail: sales@kentscientific.com Internet: www.kentscientific.com
Disclaimer
Kent Scientific Corporation makes no representations or warranties, expressed,
statutory or implied, regarding the fitness or merchantability of the components of this
system for any particular purpose. Further, Kent Scientific Corporation is not liable for
any damages, including but not limited to, lost profits, lost savings, or other incidental
or consequential damages arising from ownership or use of these products, or for any
delay in the performance of its obligations under the warranty due to causes beyond its
control. In no case shall Kent Scientific Corporation’s financial obligation extend beyond
the cost of or replacement of the Kent product in question. Kent Scientific Corporation
also reserves the right to make any improvements or modifications to these products
described in this manual at any time, without notice of these changes. All brand and
product names used in this manual are the trademarks of their respective owners.
This product is not designed, intended or authorized for use in human applications.
Product Warranty
The SomnoFlo has a one (1) year warranty including all parts and labor charges. This
warranty does not cover damage by any cause including, but not limited to, any
malfunction, defect or failure caused by or resulting from unauthorized service or parts,
improper maintenance, operation contrary to furnished instructions, shipping or transit
accidents, modifications or repair by the user, harsh environments, misuse, neglect,
abuse, accident, incorrect line voltage, fire, flood, other natural disasters, or normal
wear and tear. Changes or modifications not approved by Kent Scientific Corporation
could void the warranty. The foregoing is in lieu of all other expressed warranties. Kent
Scientific Corporation does not assume or authorize any party to assume for it any
other obligation or liability.

12
Satisfaction Guarantee
Should you experience diculty with the SomnoFlo, our Technical Support Group
will assist you in trouble-shooting and determining if the product needs to be
returned to our facility. We will issue you a Return Manufacturer Authorization (RMA)
number before the product is shipped back for repair. It is at the discretion of the
manufacturer to replace or repair a defective part or product. Please call Customer
Service at 888-572-8887 to obtain a Return Manufacturer Authorization number.
Shipments without a RMA number will not be accepted. Please note that after our
30-day return policy period ends, we will be happy to assist you with your application,
but cannot issue any credit or refund for a returned SomnoFlo.
Prior to shipment, please clean and decontaminate the product of any chemical,
biological, or isotopic contamination. Please include a completed Product Return
Form with the shipment. This form can be obtained by contacting Kent Scientific
Customer Service at 888-572-8887 or 860-626-1172.
Specifications
Voltage 12V, 2.5A
Dimensions
Weight 5.11 lbs
Input 100 to 240 V, 50 to 60 Hz, 1.0 A
Output 12V, 2.5A
5.6 x 7.7 x 10.6 inches
Environmental Intended for Indoor Use
Recommended Operating
Temperature
Relative Humidity Not exceeding 90%
Optimal Range = 18°C – 26°C (26°C Max)
Altitude 0 to 2,000 meters
Overvoltage Category II

13
Index
airflow ............................................................................ 8, 10
alarms ...................................................................................8
anest .....................................................................................7
anesthesia ................................................................. 2, 6, 8
canister ................................................................................3
charcoal ...............................................................................3
compressed gas ....................................................3, 7, 10
empty ...............................................................................7, 8
exhaust ...............................................................................10
nose cone .......................................................................4, 7
flow rate .........................................................................7, 10
induction chamber ......................................................7, 8
isoflurane ....................................................................3, 7, 8
presets .................................................................................8
purge .........................................................................7, 8, 10
settings ................................................................... 1, 2, 7, 8

1116 Litchfield Street
Torrington, Connecticut 06790
Local: 860-626-1172 Toll-Free: 888-572-8887