Kewaunee Interceptor INT-1100A2 User manual

KEWAUNEE®
...encouraging new discovery
Interceptor®
Biological Safety Cabinet
Class II A2
Maintenance and
Technical Manual
INT-1100A2 & INT-1100A2-230
INT-1400A2 & INT-1400A2-230
INT-1700A2 & INT-1700A2-230
INT-2000A2 & INT-2000A2-230
INT-1100A2-1 & INT-1100A2-1-230
INT-1400A2-1 & INT-1400A2-1-230
INT-1700A2-1 & INT-1700A2-1-230
INT-2000A2-1 & INT-2000A2-1-230
BSCMT-A2-1019

Table of Contents
CHAPTER 1
Technical Specications 1.1
CHAPTER 2
Replacement & Repair of Key Parts 2.1
Air Chamber Maintenance Item Locations 2.2
Filter Loading 2.3
Fan Failure 2.4
Fan Removal and Replacement 2.5
Pressure Transducer Failure and Replacement 2.6
CHAPTER 3
Decontamination and Repairs 3.1
Filter Replacement Instructions 3.2
Face Velocity Sensor Replacement 3.4
Thimble Connection 3.5
Parts List 3.6
CHAPTER 4
Cabinet Operation 4.1
Startup Procedure 4.3
Air Flow Patterns 4.4
Recommended Workow 4.5
NightSetback/Low Flow Mode 4.5
Control Monitor 4.6
UV Light 4.6
Control Monitor Messages and Meanings 4.7
Control Monitor Screens & Menu Diagrams 4.8
CHAPTER 5
Troubleshooting 5.1
Cabinet Calibration Instructions 5.2
Wiring Schematics 5.4

NOTICE:
The Interceptor® Biological Safety Cabinet offers many features to enhance
performance, safety, and operator comfort.
Due to the nature of work performed in a Biological Safety Cabinet, it is very
important to read the User and Operation Manual and Maintenance and Technical
Manual and follow standard operating procedures to avoid infection and other
potential injuries.
If this equipment is used in a manner not specied by the manufacturer in this
manual, the protection provided by the equipment may be impaired.
Also, any maintenance or service to an Interceptor® product must be done according
to the instructions contained herein. Maintenance of this product shall be carried out
by technicians trained in the mechanical details of this unit.
ê
!WARNING
Class II A2 Biological Safety cabinets must be certified before initial use, after
being moved, and after any service, including required annual recertification.
Service must be performed by an NSF accredited certifier using NSF/ ANSI 49
criteria and should include, at minimum, the following test:
• Downflow Velocity Profile Test
• Inflow Velocity Test
• Airflow Smoke Pattern Test
• HEPA Filter Leak Test

Maintenance and Technical Manual Chapter 1
Interceptor Biological Safety Cabinet Class II A2
October 2019 1.1
CHAPTER 1
Technical Specications for Interceptor®
Product Number
Width of
Cabinet
Height of
Cabinet
Depth of
Cabinet
Fan
Max
HP
Fan & Light
Max Current/
Power
Outlet
Max Amps
/HZ
Exhaust
CFM
INT-1100A2/INT-1100A2-1
120 VAC 1100mm
43.3"
1610mm
63.4"
813mm
32" 0.9
10.6 Amps
1275 VA
9 Amps
60hz 272
INT-1100A2-230/ INT-1100A2-1-230
230 VAC
5.2 Amps
1275 VA
4.5 Amps
50/60hz
INT-1400A2/INT-1400A2-1
120 VAC 1400mm
55.1"
1610mm
63.4"
813mm
32" 0.9
10.6 Amps
1275 VA
9 Amps
60hz 358
INT-1400A2-230/ INT-1400A2-1-230
230 VAC
5.2 Amps
1275 VA
4.5 Amps
50/60hz
INT-1700A2/INT-1700A2-1
120 VAC 1700mm
66.9"
1610mm
63.4"
813mm
32" 0.9
10.6 Amps
1275 VA
9 Amps
60hz 444
INT-1700A2-230/ INT-1700A2-1-230
230 VAC
5.2 Amps
1275 VA
4.5 Amps
50/60hz
INT-2000A2/INT-2000A2-1
120 VAC 2000mm
78.7"
1610mm
63.4"
813mm
32" 0.9
10.6 Amps
1275 VA
9 Amps
60hz 530
INT-2000A2-230/ INT-2000A2-1-230
230 VAC
5.2 Amps
1275 VA
4.5 Amps
50/60hz
Table 1.1: Product Descriptions
NOTES:
All power values measured at 10 inch operating sash height.
The powered base stand, if ordered, will take an additional 8.6 amps at 120 VAC; 60 Hz or 4.3 amps at 230 VAC;
50 Hz.
The electrical outlets inside the Interceptor® are grounded. This is particularly important since all internal surfaces
are stainless steel and conduct electricity. Under NO CIRCUMSTANCES use ungrounded plugs in these outlets.
It is not recommended to use in excess of 1500 watts of power. Exterior power plugs must not be removed until
unit fan and lights are turned o󰀨. The unit is to be disconnected from the main voltage by unplugging both plugs to
remove power. For electrically-powered base stands, power for disconnect is also accomplished by plug removal
from a waste-high or lower plug outlet.
The Interceptor® Cabinet has one power cord. If the Power Base is employed, a total of two power cords are
used. When positioning the BSC, always connect BSC and stand power plugs in waste-high or oor-positioned
outlets to facilitate disconnection in an emergency. Never block these outlets.
If UV option is on your BSC, be sure safety overrides are never immobilized! UV lamp should NEVER be on while
sash is open.
continued on page 1.2

Chapter 1 Maintenance and Technical Manual
Interceptor Biological Safety Cabinet Class II A2
October 2019
1.2
Notes: (continued)
Based on the following UL denitions, the Interceptor® may be used in a room with pollution degree 1 or pollution
degree 2 conditions:
Pollution Degree 1 No pollution or only dry, non-conductive pollution occurs. The pollution has no inuence.
Pollution Degree 2 Normally only non-conductive pollution occurs. Occasionally, a temporary conductivity
caused by condensation must be expected.
Pollution Degree 3 Conductive pollution occurs, or dry, non-conductive pollution occurs which becomes
conductive, which is expected.
Be aware that the dimensions of a Biological Safety Cabinet may exceed doorway limitations.
Sash Face Opening Chart
Model Height Width Area
INT-1100 10" 37.3" 2.59 sq ft
INT-1400 10" 49.1" 3.41 sq ft
INT-1700 10" 60.9" 4.23 sq ft
INT-2000 10" 72.7" 5.05 sq ft
Table 1.2

Maintenance and Technical Manual Chapter 2
Interceptor Biological Safety Cabinet Class II A2
October 2019 2.1
CHAPTER 2
Replacement and Repair of Key Parts
Figure 2.1 BSC with Top Front Panel open
Conditions 1, 2 and 3 mentioned in this section are all behind the Air Chamber
Panel, which is accessible by lifting the Top Front Panel. Before opening the Air
Chamber Panel, a complete decontamination of the upper plenum area is required.
This decontamination step is essential since the lters and surfaces of the upper
chamber will be contaminated.
ê
!WARNING
Opening the Air Chamber Access Panel requires a complete decontamination
of the upper plenum area. Decontamination may only be performed by an
NSF Accredited Technician.
Air
Chamber
Panel
Screw Hole
(both screws must
be removed to open
Top Front Panel)
Top Front
Panel
(shown in
open position)
Screw Hole
Factory Seal
Serial Number

Chapter 2 Maintenance and Technical Manual
Interceptor Biological Safety Cabinet Class II A2
October 2019
2.2
Air Chamber Maintenance Item Locations
Figure 2.2 BSC with Air Chamber Access Panel removed
Conditions Requiring Removal of the Air Chamber Access Panel
1) Filter Replacement
Loading of the lters to a level where an inow face velocity of at least 100
FPM cannot be attained.
2) Fan Failure
Fan fails to operate.
3) Pressure Transducer Failure
The control panel displays Sensor Error and/or erratic velocity readings.
ê
!WARNING
Opening the Air Chamber Access Panel requires a complete decontamination
of the upper plenum area. Decontamination may only be performed by an
NSF Accredited Technician.
Plenum
Access
Panel
Supply
Filter
Perforated
Panel
Exhaust
Filter
Control
Board
(on back of
Top Front
Panel)
Fan
Pressure
Transducer
(removal required for
fan replacement only)

Maintenance and Technical Manual Chapter 2
Interceptor Biological Safety Cabinet Class II A2
October 2019 2.3
Filter Loading
Over time, as the Interceptor’s lters load up, inow velocity shown on the control
screen will begin to drop. NSF 49 recommends that motor RPM be increased when
this occurs until the control unit again shows 105 FPM.
Before considering lter replacement, the motor speed control should be adjusted
to increase the air ow. Before considering lter replacement, the fan speed and top
damper should be adjusted to increase the air ow. The Fan Speed adjustment is
located under Settings|Cabinet Setup on the Control Monitor. Select Cabinet Setup
from the menu. Fan Speed is a password protected feature. Additionally you can
adjust the top damper and increase the fan speed to attain 105 FPM inow velocity.
Check the exhaust/downow balance after such an adjustment is made. The steps
must be performed by an NSF Certied Technician.
Figure 2.3a
Menu Screen
Figure 2.3b
Cabinet Setup Screen
Figure 2.3c
Fan Speed Screen
Figure 2.3d
Set Normal Fan Speed
Screen
Filters should be changed when they load up to the point that further adjustment of the
motor RPM will not increase face velocity to 100 FPM. NSF accepts a range of inow between
100-110 FPM at operating height (10" for the Interceptor).
When the static pressure difference between the negative and positive pressure
plenums reaches a certain level (generally between 1.4" and 1.8" wg depending on
BSC size and calibration), the net inow will continue to drop over time and no further
adjustment of the motor voltage will bring the face velocity back to 105 FPM.
The Filters should be replaced at this time. An inability to maintain 100 FPM mandates
lter change-out according to NSF 49.
See Chapter 3 for Filter Replacement procedures.

Chapter 2 Maintenance and Technical Manual
Interceptor Biological Safety Cabinet Class II A2
October 2019
2.4
Fan Failure
Fan failure is uncommon. First check the Blower Speed Control and the Power
Supplies which are located in the top junction box and can be replaced by a
qualied electrician without opening the Air Chamber Access Panel.
Figure 2.5 Electrical Junction Box – Blower Speed Control & Power Supplies
Once the Blower Speed Control and Power Supplies are veried and found normal,
the Air Chamber Access Panel must be removed to inspect/replace the Fan.
ê
!WARNING
Opening the Air Chamber Access Panel requires a complete decontamination
of the upper plenum area. Decontamination may only be performed by an
NSF Accredited Technician.
Blower Speed Control
24 VDC
Power Supply 24 VAC Power Supply Circuit Breakers Power Cord

Maintenance and Technical Manual Chapter 2
Interceptor Biological Safety Cabinet Class II A2
October 2019 2.5
Fan Removal and Replacement
Once the BSC is decontaminated and the Air Chamber Access Panel is removed,
removal and replacement of the fan requires the unbolting of the fan from inside the
Plenum Chamber as outlined in the steps below:
Figure 2.6 Attachment of Fan to Inner Plenum Chamber – (Model INT-1400 shown in photo)
1) Remove the exhaust lter (see lter replacement instruction)
2) Remove the Color-coded Wires from the Fan.
3) Remove the Plenum Access Cover Panel.
4) Remove the Bolts holding Fan in place. The two lower Bolts are accessible
from inside the Plenum Chamber.
5) Carefully dislodge the Fan from its cushioned Gasket and remove from the
Plenum.
6) Inspect the Fan mounting surface on the Plenum and remove any Gasket
material that may have stuck to the surface.
6) Install replacement Fan by reversing the steps above. (Make sure new Fan has
been tted with attachment nuts and new Gasket).
Replacement fans are available from Kewaunee Scientic Corporation. See
page 3.7 for part numbers.
Plenum Access
Cover Panel
FanFan Gasket
Color-coded Wires
Fan Bolts
(access from inside Plenum)
(nuts are attached to fan frame)
Fan Bolts
(removal required for
fan replacement only)

Chapter 2 Maintenance and Technical Manual
Interceptor Biological Safety Cabinet Class II A2
October 2019
2.6
Pressure Transducer Failure
Check the security of the connections at indicated locations on the BSC Control
Board. If the connections are secure, proceed with replacing the transducer.
Figure 2.7 BSC Control Board with cover removed – located on back of Front Panel
Pressure Transducer Replacement
After removing the Air Chamber Access Panel, the Pressure Transducer is located
in the far right hand side of the Air Chamber. Replacement requires disconnecting,
then reconnecting all wires and the pressure hose.
ê
!WARNING
Opening the Air Chamber Access Panel requires a complete decontamination
of the upper plenum area. Decontamination may only be performed by an
NSF Accredited Technician.
Figure 2.8 Pressure Transducer Location Figure 2.9 Pressure Transducer
Pressure
Transducer
Connection
Pressure
Transducer
Location

Maintenance and Technical Manual Chapter 3
Interceptor Biological Safety Cabinet Class II A2
October 2019 3.1
CHAPTER 3
Decontamination / Repairs
Decontamination
Upon leaving the factory, the BSC Control Monitor was calibrated and fan voltage
adjusted to the proper values:
Figure 3.1 Control Monitor – Info Screen Figure 3.2 Control Monitor – Face Velocity 105 FPM
When new, the Interceptor® Biological Safety Cabinet Control Panel shows key
values on the display. Remaining Filter Capacity should be 100%; Face Velocity
should read 105 FPM (+
- 2 FPM).
After much use, these values (remaining Filter Capacity and Face Velocity) will both
decrease. When this inevitable change occurs, some adjustment of motor RPM can
restore face velocity, but will not restore the lter capacity readout. (see Chapter 2)
Below the 50% lter capacity level, it may no longer be possible to achieve 105 FPM
by simply raising the motor RPM. Since NSF 49 requires the minimum operating
average face velocity be between 100 FPM and 110 FPM, velocity readings below 100
FPM require lter replacement.
Since both lters lie inside the contaminated Air Chamber, the BSC must be
decontaminated before opening up the panel to replace these lters.
ê
!WARNING
Opening the Air Chamber Access Panel requires a complete decontamination
of the upper plenum area. Decontamination may only be performed by an
NSF Accredited Technician.

Chapter 3 Maintenance and Technical Manual
Interceptor Biological Safety Cabinet Class II A2
October 2019
3.2
Filter Replacement
Figure 3.3 Filter Replacement Front View
Exhaust Filter
Supply Filter
Exhaust
Filter
Scissor
Clamp
Supply
Filter
Scissor
Clamp Supply Filter
Scissor Clamp
Exhaust
Filter
Scissor
Clamp
Figure 3.4 Filter Replacement End View
Exhaust Filter
Supply Filter

Maintenance and Technical Manual Chapter 3
Interceptor Biological Safety Cabinet Class II A2
October 2019 3.3
Supply and Exhaust Filter Removal Instructions
1. Lower sash to lowest position
2. Using a 5/16" hex driver, loosen and remove all hex head bolts and then
remove access panel.
Exhaust Filter Removal/Replacement
1. Using a 1/2" socket, alternately loosen the two Exhaust Filter Scissor Clamps
until Exhaust Filter can be easily removed by sliding forward.
2. Insert new Exhaust Filter and insure proper alignment.
3. Using the 1/2" socket, alternately tighten the two Exhaust Filter Scissor
Clamps until the Exhaust Filter is securely contained.
Supply Filter Removal/Replacement
1. Using a 1/2" socket, alternately loosen the two Exhaust Filter Scissor Clamps
allowing the Exhaust Filter to drop down 1/2 inch.
2. Using the 1/2" socket, alternately loosen the two Supply Filter Scissor Clamps
until the Supply Filter can be easily removed by sliding forward.
3. Insert a new Supply Filter and insure proper alignment.
4. Using the 1/2" socket, alternately tighten the two Supply Filter Scissor Clamps
until the Exhaust Filter is securely contained.
5. Using the 1/2" socket, alternately tighten the two Exhaust Filter Scissor
Clamps until the Exhaust Filter is securely contained.

Chapter 3 Maintenance and Technical Manual
Interceptor Biological Safety Cabinet Class II A2
October 2019
3.4
Replacement of Face Velocity Sensor
The thermal velocity sensor, located in the exhaust output box at the BSC top left,
is designed to measure exhaust velocity and translate it into average face velocity at
a 10" opening. If the velocity readout starts being irregular, the thermal sensor may
need replacement.
Figure 3.5 Face Velocity Sensor
Be sure the sensor window openings are perpendicular to the exhaust plane. Repair
and/or replacement of the thermal sensor can be done without decontamination
by replacing the sensor with a new unit. Once this has been done, velocity must be
recalibrated using the controller calibration program outlined in Chapter 5.

Maintenance and Technical Manual Chapter 3
Interceptor Biological Safety Cabinet Class II A2
October 2019 3.5
Thimble Connection (Canopy)
An Interceptor® Class II, A2 Biological Safety Cabinet can be used with minute
quantities of volatile toxic chemicals or trace amounts of radionuclides when properly
ducted through a Thimble Connection with Airow Sensor. The Thimble mounts to
the top of the exhaust lter guard with four (4) screws and connects to either a 8" or
10" exhaust duct. The Airow Sensor connects to the cabinet’s monitor to warn when
the exhaust ventilation is not operating.
Figure 3.6 Thimble Connection with Airow Sensor (Canopy)
Thimble Exhaust requirements
The building ventilation system must be veried that it can handle the required
exhaust volumes of the cabinet before connection. Thimble Exhaust CFM should be
1.3 times (130%) of the Biological Safety Cabinet exhaust. (See instructions shipped
with Thimble Connection for proper installation and connection procedures.)
Size of Biological
Safety Cabinet
Biological Safety
Cabinet CFM
Required Thimble
Exhaust CFM
1100mm 272 354
1400mm 358 465
1700mm 444 577
2000mm 530 689
Table 3.3
ê
!WARNING
A hood connected to a building ventilation system with a Thimble Connection
may become dysfunctional if the ventilation system fails. Check building
ventilation system before using cabinet!

Chapter 3 Maintenance and Technical Manual
Interceptor Biological Safety Cabinet Class II A2
October 2019
3.6
Replaceable Parts List
Part Numbers
Parts INT-1100 INT-1400 INT-1700 INT-2000
24 VAC Transformer F-7424-00 F-7424-00 F-7424-00 F-7424-00
24 VDC Power Supply F-7241-00 F-7241-00 F-7241-00 F-7241-00
Motor Controller F-7421-00 F-7421-00 F-7421-00 F-7421-00
Micro Switch F-7242-00 F-7242-00 F-7242-00 F-7242-00
Fluorescent / UV Relay F-7232-00 F-7232-00 F-7232-00 F-7232-00
20 Amp Circuit Breaker F-7240-00 F-7240-00 F-7240-00 F-7240-00
10 Amp Circuit Breaker F-7240-04 F-7240-04 F-7240-04 F-7240-04
0.3 Amp Circuit Breaker F-7430-00 F-7430-00 F-7430-00 F-7430-00
UV Lamp F-7249-18 F-7249-00 F-7249-00 F-7249-00
T5 Lamp F-6347-34 F-6347-46 F-7248-00 F-7248-00
Removable Work Tray 050007-36 050007-48 050007-60 050007-72
Work Tray Chrome Knob F-1145-00 F-1145-00 F-1145-00 F-1145-00
Bottom Intake Grille 052008-36 052008-48 052008-60 052008-72
Sash Glass with Handles Assembly 050009-36-FIN 050009-48-FIN 050009-60-FIN 050009-72-FIN
Exhaust HEPA filter F-7275-36-FIN F-7275-48-FIN F-7275-60-FIN F-7275-72-FIN
Supply HEPA filter F-7276-36-FIN F-7276-48-FIN F-7276-60-FIN F-7276-72-FIN
Removable Work Tray Support 052063-00 052063-00 052063-00 052063-00
Sash Sweeper Plate 052076-36 052076-48 052076-60 052076-72
Top Exhaust Balance Damper 052077-00 052077-00 052077-00 052077-00
Damper Telescoping Section 050166-00 050166-00 050166-00 050166-00
Downflow Perforated Grille 050079-36 050079-48 050079-60 050079-72
Arm Rest Bracket 050195-0A 050195-0A 050195-0A 050195-0A
Arm Rest Plastic End Cap F-7279-00 F-7279-00 F-7279-00 F-7279-00
Aluminum Arm Rest F-7280-36 F-7280-48 F-7280-60 F-7280-72
Drain Valve Plate 050110-00 050110-00 050110-00 050110-00
316 SS Pipe Fitting F-7211-11 F-7211-11 F-7211-11 F-7211-11
316 SS Ball Valve F-7211-00 F-7211-00 F-7211-00 F-7211-00
Safety Labels 120 VAC F-8200-01 F-8200-01 F-8200-01 F-8200-01
Pressure Transducer F-7433-00 F-7433-00 F-7433-00 F-7433-00
Velocity Sensor F-7425-00 F-7425-00 F-7425-00 F-7425-00
Fan Assembly F-7420-00 F-7420-00 F-7420-00 F-7420-00
Gas Spring Assembly F-7224-00 F-7224-00 F-7223-00 F-7223-00
Belt Drive to Counterweight F-6343-00 F-6343-00 F-6343-00 F-6343-00
Table 3.2

Maintenance and Technical Manual Chapter 4
Interceptor Biological Safety Cabinet Class II A2
October 2019 4.1
CHAPTER 4
Cabinet Operation
Theory of Operation
The Interceptor® is a Class II, A2 Biological Safety Cabinet. Its function is to
isolate bacterial samples from cross-contamination and protect the user and
the environment around the cabinet from being contaminated by biological or
particulate material inside the cabinet.
It accomplishes this objective by bathing biological samples in HEPA ltered clean
air while keeping contaminants contained by having a continuous substantial inow
of air through the 10” cabinet sash opening.
The BSC will safely operate at a temperature range of 55ºF to 85ºF (13ºC - 29ºC) and
a relative humidity of 10% to 70%.
Class II, A2 Biological Safety Cabinets are made to safely contain bacterial samples
that fall into the Biological Safety levels 1, 2, 3, and 4 if accompanied by level
appropriate protection garments. The following is a description of each level as
published in the CDC BMBL 5th edition:
“Biological Safety level 1 (BSL-1) is the basic level of protection and is
appropriate for agents that are not known to cause disease in normal,
healthy humans. Biological Safety level 2 (BSL-2) is appropriate for
handling moderate-risk agents that cause human disease of varying
severity by ingestion or through percutaneous or mucous membrane
exposure. Biological Safety level 3 (BSL-3) is appropriate for agents with
a known potential for aerosol transmission, for agents that may cause
serious and potentially lethal infections and that are indigenous or
exotic in origin. Exotic agents that pose a high individual risk of life-
threatening disease by infectious aerosols and for which no treatment
is available are restricted to high containment laboratories that meet
biosaftey level 4 (BSL-4) standards.”
ê
!WARNING
Class II A2 Biological Safety cabinets must be certified before initial use, after
being moved, and after any service, including required annual recertification.
Service must be performed by an NSF accredited certifier using NSF/ ANSI 49
criteria and should include, at minimum, the following test:
• Downflow Velocity Profile Test
• Inflow Velocity Test
• Airflow Smoke Pattern Test
• HEPA Filter Leak Test

Chapter 4 Maintenance and Technical Manual
Interceptor Biological Safety Cabinet Class II A2
October 2019
4.2
Touch Pad Contol Monitor
Home/Face Velocity Screen
Asset Tag
Face Velocity
Local Time
12h /24hr
Unit of Measure
FPM or M/S
UV Light ON/OFF
Button
Information
Button
Alarm LED
Home
Button
Warnings & Alarms
Latch Banner
Light ON/OFF
Button
Fan ON/OFF
Button
Settings
Button
Alarm Mute
Button
Figure 4.1 Touch Pad Control Monitor