Kurabo QuickGene-Auto240L User manual

Automated Nucleic Acid Isolation System
QuickGene-Auto240L
Ver. 1.0.1
Operation Manual

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QuickGene-Auto240L Operation Manual
Introduction
Thank you very much for purchasing our Automated Nucleic Acid Isolation System QuickGene-Auto240L
(hereafter referred to as "this system"). This document contains important information about correct and safe
use of the functions of this system. Before using, be sure to read this document. For quick reference, please
keep this document within easy reach of this system.
Application of Infectious Waste Rules
If a potentially infectious sample is to be disposed of after use, it should be treated with
incineration, melting, sterilization, disinfection, etc. in accordance with the rules at your
facility (institute) or relevant laws because it is classified as infectious industrial waste.
If disposal is outsourced, use a licensed contractor as a waste disposer of industrial
waste subject to special control together with a manifest of industrial waste subject to
special control.
■Disclaimer
・The contents of this document are subject to change without prior notification.
・Our company shall not be liable for:
- Any violation of patent right of a third party or other rights through use of data described in
document.
- Any failure or damage caused by installation, relocation, remodeling, maintenance or repair
carried out by any party other than our company or other than a contractor designated by our
company
- Any failure or damage of our product caused by a product delivered by other than our
- Any failure or damage caused by remodeling, maintenance, repair, etc. using any parts other
than genuine parts designated by our company
- Any failure or damage resulting from non-compliance with precautions and operation
procedures described in this document
- Any failure or damage due to deviation in surrounding conditions from the conditions of use of
this system, such as power source or installation environment
- Any failure or damage caused by fire or natural disasters such as earthquake, flood, lightning,
etc.
・Unauthorized reproduction in whole or in part of the contents of this document is prohibited.
Biohazard Symbol

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How to Read This Document
Warning Labels
The precautions required for the safe use of this system are described in this document.
Be sure to read the precautions and thoroughly understand the contents before using this system.
SAFETY SYMBOLS:
"Warning" indicates the risk of death or serious injury if not avoided.
"Warning" indicates a risk of death or serious injury.
"Biohazard" indicates a risk of infection.
"Caution" indicates a risk of lighter or moderate injury, or property damage.
Important: "Important" indicates critical or prohibited actions.
Note: "Note" indicates precautions, instructions to abide by, supplemental explanations, etc.
Warning:
Biohazard:
Caution:

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QuickGene-Auto240L Operation Manual
Precautions for Use
For safe use of this system, please abide by the safety descriptions below.
Symbol
Please check all contents carrying this symbol.
System
・If the following are not abided by, fire or electric shock may result.
- Use the power cable supplied with this system.
- Connect to a power outlet with a grounding terminal.
- Do not handle the power plug with wet hands.
- Do not use with a voltage other than the indicated voltage, and avoid branch connections or
extension cords.
- Do not use with a damaged power plug or loose outlet.
- With a dry cloth, wipe off any dust completely from the power plug electrode and the outlet.
- Do not pull on the power cable, but be sure to grip the power plug to remove from the outlet.
- Remove the power plug from the outlet for safety during maintenance work.
- Do not touch the power plug when thunder is heard or lightning is seen.
- When a breaker is shut off, leakage is possible. Do not disassemble this system.
・Do not spill liquid on this system. Do not place a container of liquid on this system. It may
failure, fire or electric shock.
・If heat, smoke or a strange odor is generated by the system, remove the power plug from the
outlet.
・Any remodeling of this system without authorization by our company is prohibited. Fire or
electric shock may result.
・Do not place any object on this system. Do not drop any object onto or cause shock to the
system. A system failure or malfunction may occur.
・Do not ride above this system. Falling and injury may occur.
・If any liquid, etc. adheres to this system, promptly wipe it off with soft paper, etc. System
damage may result.
・The sliding doors, flap doors and drawers are not fixed halfway. Releasing halfway or leaving
them open will cause injury or system failure.
・In this system, the noise peak during operation is more than 63dB(A) and less than 70dB(A).
・Wear protective gloves when replacing consumables.
・If ethyl alcohol is spilled, promptly remove power plug from outlet.
・Repair of this system must be conducted by a contractor designated by our company.
・Use genuine parts designated by our company.
・The repair of this system shall be conducted by a contractor designated by our company.
・Use reagents and consumables included in our genuine parts and reagent kits.
Warning:
Caution:

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Overvoltage Categories
This system is an overvoltage category II device.
Be sure to use the power cable delivered with the system.
NB: Overvoltage category II indicates a device that consumes energy supplied from
stable wiring (a permanent outlet, etc.)
QuickGene-Auto240L Kit
(Hereafter, the QuickGene-Auto240L Kit is referred to as a "dedicated kit" in this document.)
・Refer to the procedures for use of the dedicated kit in the handbook attached to the kit.
・Handle the reagents in the kit in accordance with the safety precautions for handling and use.
・Use in a laboratory or work space suitable for isolation work.
QuickGene-Auto240L Kit List
Whole Blood DNA Reagent Kit
QuickGene DNA whole blood kit L (DB-L)
Consumable Supply Kit
QuickGene-Auto240LConsumables Kit (QG-240L-CK)
NB: These products are not included in this system package. Please purchase separately.
Reagents Used for Other than Dedicated Kits
・For reagents used for other than a dedicated kit, the work should be carried out in
accordance with the safety precautions for handling and use of each reagent.
・Use in a laboratory or work space suitable for isolation work. Some reagents must be
prepared in a draft chamber.
Cleaning Agents for Cleaning or Washing
・Work with agents for cleaning or washing the main unit and its accessories should be
conducted in accordance with the safety precautions for handling and use of each
cleaning agent.
・Ethyl alcohol is an inflammable substance. Do not use in the proximity of open fire.
・Wear proper gloves, mask and protective goggles for cleaning work.
Samples
Use proper gloves, mask and protective goggles for handling samples with a risk of
infection.
Caution:
Biohazard:
Biohazard:
Warning:
Caution:
Caution:

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QuickGene-Auto240L Operation Manual
Laser Standards
The laser sensor used in this system for detecting the workpiece/consumables is classified as "Class 1"
according to standard IEC60825-1 in accordance with stipulation in FDA Laser Notice No. 50.
The barcode reader (light source component) for reading the barcode on blood sample collection tubes
is classified as “Exempt Group”in accordance with standard IEC62471.
The 2-dimensional barcode reader (target pointer component for reading position adjustment) for
reading the code on collection tubes is classified as "Class 1" in standard IEC60825-1 in accordance
with stipulation in FDALaser Notice No. 50.
NB: The 2-dimensional barcode reader is an optional component for this system. It is implemented
only when included as an option.
Do not attempt to peer into the system and directly view the barcode reader or
2-dimensional code reader red light source during the sample ID reading operation.
Direct viewing at length may cause eye disorders.
Operation with a procedure other than indicated in the operation manual may lead
to exposure to dangerous laser irradiation.
Laser Device Specification
Specification/Name
Laser Sensor
(for detection of workpiece/consumables)
2-Dimensional Code Reader
(for reading collection tube information)
Wavelength (nm)
655
660
Output (mW)
0.39
0.06
Coverage Angle (º)
X direction: 0.20
Y direction: 0.53
－
Laser Class
CLASS 1 Laser Product
(IEC60825-1：2007
FDA (CDRH) Part 1040.10)
CLASS 1 Laser Product
(IEC60825-1：2007
FDA (CDRH) Part 1040.10)
Ultraviolet Light (UV) Irradiating Function
Operation of the UV irradiating function of this system will cause a small quantity
of ultraviolet leakage from the sliding door window, fan area, joints with exteriors,
etc. Exposure to ultraviolet light may cause damage such as eye or skin irritation.
Avoid exposure of skin or eyes to ultraviolet light.
Warning:
Warning:
Warning:

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Warning Labels
Warning, caution, instruction and obligation labels are affixed to this system.

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QuickGene-Auto240L Operation Manual
: Wear protective equipment
This system may become contaminated with infectious substances, etc. When inserting your hands into
the system, be sure to wear protective gloves.
: Biohazard Risk Label
This label indicates a biohazard risk.
Warning: Hot Surface
Risk of burns: do not touch immediately after operation.
Warning: Laser Light
Direct viewing of the laser light source may cause eye damage. Do not directly view the laser light at
length.
Warning: UV
Do not work for a long time in the proximity during UV irradiation. Risk of damage to eyes and skin.
Caution: Damage
Do not apply force to the flap door by leaning or sitting on it or by placing any objects on it. Doing so
may cause damage or injury.

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Restrictions on Use
This section indicates the restrictions on use of QuickGene-Auto240L and QuickGene Consumables.
・No liability is assumed for the results regardless of the purpose of use.
・Validation of performance on samples shall be based on the judgment and responsibility of the
user.
When using this system, please thoroughly read this document and abide by the safety rules of your facility.

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QuickGene-Auto240L Operation Manual
Contents
1 System Installation Procedures p. 1
1.1 Conditions for Installation, Operating Environment
1.2 How to Open the Package
1.3 How to Remove the Interior Packing Material and Fixtures
1.4 Checking of Packed Contents
1.5 Names of Parts
1.6 Mounting of UV Lamp
1.7 Checking the System Functions
1.8 Precautions for Storage
2 Basic Functions p. 11
2.1 Components Configuring the System
2.2 Basic Operations, Basic Functions
2.3 Safety Mechanisms
2.4 Operation Panel Displaying Contents
2.5 Barcode Scanning Functions
2.6 Basic Specifications
2.7 Product Labeling
2.8 Implemented Protocols
3 How to Operate (Full-Automatic Protocol) p. 31
3.1 For Isolating Operation
3.2 Confirmation of Articles to be Prepared
3.3 Preparation of Reagents
3.4 Preparation of Consumables and Accessories
3.5 Preparation of Samples
3.6 Start-up of System
3.7 Registration and Deletion of User IDs
3.8 Isolating Operation
3.9 Operation to Stop Automatic Operation
4 Operating Procedures (Semi-Automatic Protocol) p. 65
4.1 For Isolating Operation
4.2 Confirmation of Articles to be Prepared

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4.3 Preparation of Reagents
4.4 Preparation of Consumables and Accessories
4.5 Preparation of Samples
4.6 Isolating Operation
5 Operation History p. 85
5.1 Checking Operation History
5.2 Checking ID Information
5.3 Storing Operation History
6 Parameter Setup Procedures p. 91
6.1 Parameters
6.2 Starting Up the Parameter Setup Mode
6.3 Setup of Barcode (ID) Reading Function
6.4 Setup of DNA Elution Buffer Volume
6.5 Parameters Setup/Changed with the EXPERT Mode
7 Daily Inspections and Maintenance p. 101
7.1 What to Do before Using the System
7.2 When System Not in Use for More than One Week
7.3 Cleaning of System Main Unit and Accessories
7.4 Replacement of Consumables
7.5 Cleaning of Wash Buffer Feeding Line
7.6 UV (Ultraviolet Light) Irradiating Function
8 Before Concluding as a Failure p. 127
8.1 Troubleshooting
8.2 Error Messages
Appendix A p. 141
A.1 Options
A.2 Warranty
A.3 After-Sales Services
A.4 Customer Consultation Desk
A.5 Precautions for Transportation
A.6 Disposal

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QuickGene-Auto240L Operation Manual
INDEX
The system installation procedures are explained.
The basic functions are listed.
The Isolating operation for full-automatic operation protocol is explained.
The Isolating operation for semi-automatic operation protocol is explained.
The checking and storage procedures for system operation histories are explained.
The procedures for setting up parameters are explained.
The procedures for daily inspection and maintenance are explained.
The handling procedures when you consider a failure has occurred are explained.
The warranties, after-sales services and precautions for transportation are explained.
1
2
3
4
5
6
7
8
A
1 System Installation Procedures
2 Basic Functions
3 How to Operate (Full-Automatic Protocol)
4 Operating Procedures (Semi-Automatic Protocol)
5 Operation History
6 Parameter Setup Procedures
Appendix A
7 Daily Inspections and Maintenance
8 Before Concluding as a Failure

xii QuickGene-Auto240L Operation Manual

1 System Installation Procedures
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QuickGene-Auto240L Operation Manual
1
1 System Installation Procedures
The installation procedures for this system are explained below.
1.1 Conditions for Installation, Operating Environment
The conditions for system installation and the operating environment are explained here.
■ Conditions for Installation
・Do not install this system where water may be poured or spilt, which may
failure, fire or electric shock.
・Be sure to remove the power plug from the outlet whenever this system is
moved. Watch your step carefully while operating. Injury, fire or electric
may result from a damaged power cable.
・Do not install this system in a location with intense vibration or at an unstable
incline. This may cause injury or failure.
・Do not install this system in a location exposed to direct sunlight or in the
proximity of a heating or cooling appliance. This may cause a shortened
lifetime or failure.
Please install this system in an indoor location as shown below for normal, safe use.
・A location with the following secure space for installation.
Required footprint: Width ≧178 cm × Depth ≧152 cm × Height ≧205 cm (when placed on an
80-cm high experiment table)
Warning
:
Caution:
10cm
(40 cm)
(20 cm)
(10 cm)
(70 cm)
(10 cm)
205 cm
178 cm
152 cm

1 System Installation Procedures
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A location with a secure power source (This system is an overvoltage category II device. Be sure to use the
included power cable.)
・Operating temperature 15°C-30°C, room humidity 30%-80% (no condensation)
・A stable horizontal location with load-bearing capacity of ≧350kg and minimal vibration.
・A location not exposed to direct sunlight or strong light (Use a curtain, blind, etc. for obstructing
light as necessary.)
・A location with good ventilation and minimal dust
・A location with no abrupt variation in temperature (Swift heating of a very cold room or moving
this system from a lower temperature to a warm place, etc. will generate water droplets in the
system (condensation), and the isolation result will be adversely influenced.)
・A location where temperature and humidity within the specified ranges can be maintained (Water
faucets, boilers, coolers, heaters, etc. should not be in proximity.)
・A location where strong magnetism (motors, transformers, televisions, speakers, magnets, etc.) is
not in proximity (Approaching a magnetic source will cause malfunction.)
■Operating Environment
Item
Specification
Temperature (oC)
In operation
15-30
During standby
15-30
Humidity (%)
In operation
30-80
During standby
10-80
Max. Wet-bulb Temp.
(oC)
In operation
29 (no condensation)
During standby
29 (no condensation)
Temperature Gradient
(oC / Hr)
≦12 (no condensation)
Humidity Gradient
(% / day)
≦30 (no condensation)
Altitude (m)
Use at ≦1600

1 System Installation Procedures
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QuickGene-Auto240L Operation Manual
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1.2 How to Open the Package
Refer to the separately attached "QuickGene-Auto240L unpacking instruction"

1 System Installation Procedures
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1.3 How to Remove the Interior Packing Material and Fixtures
Refer to the separately attached "QuickGene-Auto240L Fixture Removing Procedures".

1 System Installation Procedures
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QuickGene-Auto240L Operation Manual
1
1.4 Checking of Packed Contents
Please check that the accessories, operation manual and warranty certificate for the system main unit are
included in the box.
If any item is missing, please contact our customer consultation desk.
□ System Main Unit
□ Operation Manual
□ Power Cable
□ Reagent Container Holder
□ Sample Tip Holder
□ Reagent Tip Holder
□ Sample Holder ×3
□ Cartridge Holder ×3
□ Waste Tube Holder ×3
□ Collection Tube Holder ×3
□ Waste Container
□ Wash Buffer Bottle
□ Reagent Container S ×3
□ Reagent Container L ×4
□ UV Lamp
Caution:
Note: Use power cables, holders, containers included in
package

1 System Installation Procedures
6 QuickGene-Auto240L Operation Manual
1
1.5 Names of Parts
The names of system parts and their functions are explained below.
■System Front View
■System Side View
Operation Panel
Power Switch
Drawer
Flap Door (R)
Power Cable
Sliding Door
Flap Door (L)
Right Side
Left Side
Inlet
Power Cable
USB
Port 2
USB
Port 1
LAN
Port
Serial Port
(9 pin)
Power Cable Not Connected
Power Cable Connected