Kurabo QuickGene-Auto12S User manual

QuickGene-Auto12S
QuickGene-Auto24S
Ver. CEIVD2.0
Operation Manual

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QuickGene-Auto12S/QuickGene-Auto24S Operation Manual
Introduction
Thank you very much for purchasing our Automated Nucleic Acid Isolation Device QuickGene-Auto12S /
QuickGene-Auto24S. This document contains important information about correct and safe use of the
functions of this device. Before using, be sure to read this document. For quick reference, please keep this
document within easy reach of this equipment.
Application of Infectious Waste Rules
If a potentially infectious sample is to be disposed of after use, it should be treated with
incineration, melting, sterilization, disinfection, etc. in accordance with the rules at your
facility (institute) or relevant laws because it is classified as infectious industrial waste.
If disposal is outsourced, use a licensed contractor as a waste disposer of industrial
waste subject to special control together with a manifest of industrial waste subject to
special control.
■Disclaimer
・The contents of this document are subject to change without prior notification.
・Our company shall not be liable for:
- Any violation of patent right of a third party or other rights through use of data described in this
document.
- Any failure or damage caused by installation, relocation, remodeling, maintenance or repair carried out
by any party other than our company or other than a contractor designated by our company
- Any failure or damage of our product caused by a product delivered by other than our company
- Any failure or damage caused by remodeling, maintenance, repair, etc. using any parts other than
genuine parts designated by our company
- Any failure or damage resulting from non-compliance with precautions and operation procedures
described in this document
- Any failure or damage due to deviation in surrounding conditions from the conditions of use of this
system, such as power source or installation environment
- Any failure or damage caused by fire or natural disasters such as earthquake, flood, lightning, etc.
・Unauthorized reproduction in whole or in part of the contents of this document is prohibited.
Biohazard Symbol

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QuickGene-Auto12S/QuickGene-Auto24S Operation Manual
How to Read This Document
Warning Labels
The precautions required for the safe use of this system are described in this document.
Be sure to read the precautions and thoroughly understand the contents before using this system.
SAFETY SYMBOLS:
"Warning" indicates the risk of death or serious injury if not avoided.
"Warning" indicates a risk of death or serious injury.
"Biohazard" indicates a risk of infection.
"Caution" indicates a risk of lighter or moderate injury, or property damage.
Important: "Important" indicates critical or prohibited actions.
Note: "Note" indicates precautions, instructions to abide by, supplemental explanations, etc.
Intended Use
QuickGene-Auto12S / QuickGene-Auto24S with QuickGene-AutoS DNA Blood Kit is intended to isolate high-quality
genomic DNA automatically from human whole blood sample.
Generally, DNA isolated by the system is useful for PCR based analysis like HLA typing or karyotyping to know
patients’ genotype before transplantation, and for Next Generation Sequencing (NGS) for selection of the
molecular-targeted agents. Such high-quality genomic DNA is also suitable for the long-term storage project like bio
banking with less DNA degeneration/ degradation. DNA isolated from the system can’t be used for diagnosis,
prevention, or treatment of a disease purpose directly.
The system and the kit are intended for use by professional users; adequately skilled in molecular biological techniques
and trained to operate the system.
Warning:
Biohazard:
Caution:

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QuickGene-Auto12S/QuickGene-Auto24S Operation Manual
Precautions for Use
This manual is designed to assist you with the operation of the QuickGene-Auto12S / QuickGene-Auto24S
system in advance. Read it thoroughly before using the equipment or beginning any maintenance on it. These
WARNINGS and CAUTIONS are intended to protect you and other persons from injuries and damages.
Ignoring the following notations may lead to fire or electric shock. To ensure safety operation, please follow
them carefully.
Symbol
Please check all contents carrying this symbol.
Device
-Make sure you get the power cable that meets your country’s standard, in case of doubt,
contact your local distributor.
-Make sure to connect to a power outlet with a grounding terminal.
-Do not handle the power plug with wet hands.
-Do not use with a voltage other than the indicated voltage, and avoid branch connections or
extension cords.
-Do not use with a damaged power plug or a loose socket.
-If there is dust on the prongs of the power plug or on the plug socket, remove it with a dry
cloth.
-When you disconnect the plug from the outlet, be sure to hold the power plug itself. Do not
pull the power cable.
-For maintenance, disconnect the power plug from the outlet.
-Do not pour any liquid on or in the device.
-Do not place any objects containing liquid on the device. Doing so may cause a device
failure, fire, or electric shock.
-In the event the device overheats, starts to smoke or smells abnormal, immediately unplug
the power cable.
-Do not touch the power plug when thunder is heard or lightning is seen.
-When the current-limiting fuse is broken, leakage is possible. Do not use this equipment.
Warning:

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QuickGene-Auto12S/QuickGene-Auto24S Operation Manual
-Never attempt to alter QuickGene-Auto12S / QuickGene-Auto24S without the
manufacturer’s permission. Doing so may cause fire or electric shock.
-Do not place or drop objects on the device. Blunt force impact to the device
may cause failure or malfunction of QucickGene-Auto12S / QuickGene-Auto24S.
-Liquid spills inside the device should be wiped up immediately as potential damage to
electrical systems can occur.
-Do not ride above the device. Falling and injury may occur.
-Wear protective gloves when replacing consumables.
-If ethyl alcohol is spilled, promptly remove power plug from outlet.
-Repairs to QucickGene-Auto12S / QuickGene-Auto24S should only be performed by such
agencies as are specifically authorized by KURABO Industries., Ltd.
-Only original KURABO Industries., Ltd. replacement parts should be used.
Instruction for handling hazardous substances
When handling or disposing of infectious materials, follow local municipal waste ordinances for proper disposal
provisions to reduce the environmental impact of waste electrical and electronic equipment (WEEE).
QuickGene-Auto12S / QuickGene-Auto24S Kit
(Hereafter, the QucickGene-Auto12S / AuickGene-Auto24S Kit is referred to as a "dedicated kit" in this document.)
・Refer to the procedures for use of the dedicated kit in the handbook attached to the kit .
・Handle the reagents in the kit in accordance with the safety precautions for handling and use.
・Use in a laboratory or work space suitable for isolation work.
QuickGene-Auto12S/24S Kit List
Blood DNA Reagent Kit
QuickGene-AutoS DNA Blood kit
NB: These products are not included in this system package. Please purchase separately.
Reagents Used for Other than Dedicated Kits
・For reagents used for other than a dedicated kit, the work should be carried out in
accordance with the safety precautions for handling and use of each reagent.
・Use in a laboratory or work space suitable for isolation work. Some reagents must be
prepared in a draft chamber.
Caution:
Caution:
Caution:

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QuickGene-Auto12S/QuickGene-Auto24S Operation Manual
Cleaning Agents for Cleaning or Washing
・Work with agents for cleaning or washing the main unit and its accessories should be
conducted in accordance with the safety precautions for handling and use of each
cleaning agent.
・Ethyl alcohol is an inflammable substance. Do not use in the proximity of open fire.
・Wear proper gloves, mask and protective goggles for cleaning work.
Samples
Use proper gloves, mask and protective goggles for handling samples with a risk of
infection.
Ultraviolet Light (UV) Irradiating Function
Operation of the UV irradiating function of this system will cause ultraviolet (small
amount) leakage from the front door and its peripheral part, joints with exteriors,
etc. Exposure to ultraviolet light may cause damage such as eye or skin irritation.
Avoid exposure of skin or eyes to ultraviolet light.
Caution:
Biohazard:
Biohazard:
Warning:

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QuickGene-Auto12S/QuickGene-Auto24S Operation Manual
Warning Labels
Warning, caution, instruction and obligation labels are affixed to this system.
: Wear protective equipment
QuickGene-Auto12S / QuickGene-Auto24S may become contaminated with infectious substances, etc. When
inserting your hands into the device, be sure to wear protective gloves.
: Read operation manual
Read and understand operation manual before using this equipment.
: Biohazard Risk Label
This label indicates a biohazard risk.
Warning: Hot Surface
Risk of burns: do not touch immediately after operation.
Warning: hands pinching
Keep hands away.
Warning: Hazardous voltage
Risk of electrical shock.
Warning: UV
Risk of damage to eyes and skin.

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QuickGene-Auto12S/QuickGene-Auto24S Operation Manual
Markings
Mark
Description
Mark
Description
Manufacturer information
Consult instructions for use
SN
Serial number
European Authorized
Representative
Do not dispose of this product
as unsorted municipal waste
Humidity limitation
Caution! Consult
accompanying documents
Temperature limitation
Recyclable materials
In Vitro Diagnostic
Medical Device
CE Marking
Product Name Label
QuickGene-Auto12S QuickGene-Auto24S

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QuickGene-Auto12S/QuickGene-Auto24S Operation Manual
Restrictions on Use
This section indicates the restrictions on use of QuickGene-Auto12S / QuickGene-Auto24S and dedicated kit.
・No liability is assumed for the results regardless of the purpose of use.
・Validation of performance on samples shall be based on the judgment and responsibility of the
user.
When using this device, please thoroughly read this document and abide by the safety rules of your facility.

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QuickGene-Auto12S/QuickGene-Auto24S Operation Manual
Contents
1 Installation Procedures p. 1
1.1 Conditions for Installation, Operating Environment
1.2 How to Open the Package
1.3 Checking of Packed Contents
1.4 Device Overview
1.5Safety Function
1.6 Implemented Protocols
1.7 Mounting of UV Lamp
2 How to Operate p. 13
2.1 Confirmation of Articles to be Prepared
2.2 Preparation of Reagents
2.3 Preparation for Operation
2.4 Operation of DNA/RNA Isolation
2.5 Collection of Isolation Samples
2.6 After Operation
3 Cleaning and Maintenance p. 27
3.1 Daily Maintenance
3.2 Weekly Maintenance
3.3 Half a Year Maintenance
3.4 How to replace the power fuse
4 Before Concluding as a Failure p. 31
4.1 Troubleshooting
Appendix A p. 33
A.1 Options
A.2 Warranty
A.3 After-Sales Services
A.4 Customer Consultation Desk
A.5 Precautions for Transportation
A.6 Disposal

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QuickGene-Auto12S/QuickGene-Auto24S Operation Manual

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QuickGene-Auto12S/QuickGene-Auto24S Operation Manual
INDEX
The device installation procedures are explained.
The isolation operation is explained.
The procedures for cleaning and maintenance are explained.
The handling procedures when you consider a failure has occurred are explained.
1
2
3
4
1 Installation Procedures
2 How to Operate
3 Cleaning and Maintenance
4 Before Concluding as a Failure

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QuickGene-Auto12S/QuickGene-Auto24S Operation Manual

1 Installation Procedures
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QuickGene-Auto12S/QuickGene-Auto24S Operation Manual
1
1 Installation Procedures
The installation procedures for this device are explained below.
1.1 Conditions for Installation, Operating Environment
The conditions for system installation and the operating environment are explained here.
■ Installation location
・To avoid the risk of electric shock, this equipment must only be connected to a
supply mains with protective earth.
-Install QuickGene-Auto12S / QuickGene-Auto24S on a clean, dry, sturdy
surface within 1.2 M of an electrical outlet, and clearance around the
instrument base at all times to ensure proper ventilation.
-Do not install this device in a location with intense vibration or at an unstable
incline. This may cause injury or failure.
-Do not install this device in a location exposed to direct sunlight or in the
proximity of a heating or cooling appliance. This may cause a shortened
lifetime or failure. (Block the sunlight by closing curtains or blinds as
necessary).
-Install QuickGene-Auto12S / QuickGene-Auto24S on a location which is
well-ventilated and not dusty.
-Install QuickGene-Auto12S / QuickGene-Auto24S on a location with
minimal sudden temperature fluctuation (Warming a cold room suddenly or
moving the device from a room with low temperature to a warm room may
cause condensation inside the device, resulting in sub-standard performance.)
-Install QuickGene-Auto12S / QuickGene-Auto24S on a location where the
temperature and humidity are kept within the specified range (far from water
faucets, water heaters, humidifiers, air-conditioners and heaters)
-Do not install QuickGene-Auto12S / QuickGene-Auto24S on a location
where strong magnetism (motors, transformers, televisions, speakers, magnets,
etc.) is in proximity (Approaching a magnetic source will cause malfunction.)
Warning
:

1 Installation Procedures
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QuickGene-Auto12S/QuickGene-Auto24S Operation Manual
1
■ Installation location of QuickGene-Auto12S
Please install this device in an indoor location as shown below for normal, safe use.
Required footprint: Width ≧68 cm × Depth ≧92 cm × Height ≧79 cm
■ Installation location of QuickGene-Auto24S
Please install this device in an indoor location as shown below for normal, safe use.
Required footprint: Width ≧103 cm × Depth ≧92 cm × Height ≧80 cm
(20 cm)
(10 cm)
(10 cm)
(20 cm)
(10 cm)
79 cm
92 cm
68 cm
(20 cm)
(10 cm)
(10 cm)
(10 cm)
(20 cm)
80 cm
103 cm
92 cm

1 Installation Procedures
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QuickGene-Auto12S/QuickGene-Auto24S Operation Manual
1
■ Specifications
Item
QuickGene-Auto12S
QuickGene-Auto24S
Dimension (W x D x H)
480 x 620 x 590 mm
830 x 620 x 590 mm
Weight
55 kg
80 kg
Throughput
1-12 sample/run
1-24 sample/run
Power Supply
100-240V±10%, 50/60 Hz
Mainboard
PCB
UV lamp
UV-C
Light source
LED white light
Heating block
25-60°C
User’s interface
7” TFT LCD touch screen (800*400)
■Operating environment
Item
Specification
Temperature
15–30°C
Humidity
30-80 % relative humidity, non-condensing
Input power
100-240 V/50-60Hz
Overvoltage category
definition
Transient overvoltage category II
Table tolerance weight
QuickGene-Auto12S: ≧55 kg
QuickGene-Auto24S: ≧80 kg
■Operating parameters
Item
Specification
Processing time
20-50 mins (Depend on protocol)
Sample volume
200 µl (Can be customized)
Elution volume
50 µl, 100 µl, 150 µl, 200 µl

1 Installation Procedures
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QuickGene-Auto12S/QuickGene-Auto24S Operation Manual
1
1.2 How to Open the Package
Don’t carry the equipment by alone.
2 or more people are needed for carrying QuickGene-Auto12S.
4 or more people are needed for carrying QuickGene-Auto24S.
Follow the instructions to unpack the device.
1.2.1 Remove the outer carton, four foam pieces, and take out the device.
Wooden carton dimension
-QuickGene-Auto12S: 70 x 85 x 85 cm, GW: 105 kg
-QuickGene-Auto24S: 85 x 104 x 93 cm, GW: 143 kg
Caution:

1 Installation Procedures
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QuickGene-Auto12S/QuickGene-Auto24S Operation Manual
1
1.2.2 Remove the foam between the Reagent holder and syringe head from inside the instrument
and cut the cable tie of Reagent holder.
1.2.3 Remove the foam between the Y2 axis and worktable from inside the instrument and cut the
cable tie of Collection holder.
1.2.4 Take out the Collection holder, remove the wing screw and take out the UV lamp, then install it.

1 Installation Procedures
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QuickGene-Auto12S/QuickGene-Auto24S Operation Manual
1
1.2.5 Plug in the Power Cable and turn on the rear power switch.
1.2.6 Turn on the main switch (Orange).
Do not obstruct the mains plug or position the equipment where the connection to
the mains line can be accidentally disconnected.
Warning: