Life technologies EVOS XL Core User manual

5
EVOS®XL Core
Imaging System
for Transmitted Light Applications
Catalog Number AMEX1000
Doc. Part no. ZP-PKGA-0583
Pub. no. MAN0007718
Rev. 1.0
For Research Use Only. Not for use in diagnostic procedures.
QUICK REFERENCE
Limited Product Warranty
Life Technologies Corporation and/or its affiliate(s) warrant their products as set forth in the Life Technologies’ General Terms and
Conditions of Sale found on Life Technologies’ website at www.lifetechnologies.com/termsandconditions. If you have any questions,
please contact Life Technologies at www.lifetechnologies.com/support.
DISCLAIMER
LIFE TECHNOLOGIES CORPORATION AND/OR ITS AFFILIATE(S) DISCLAIM ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT,
EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY, FITNESS FOR A PARTICULAR
PURPOSE, OR NON-INFRINGEMENT. TO THE EXTENT ALLOWED BY LAW, IN NO EVENT SHALL LIFE TECHNOLOGIES AND/OR
ITS AFFILIATE(S) BE LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER BASIS
FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING
FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE USE THEREOF.
Limited Use Label License: Research Use Only
The purchase of this product conveys to the purchaser the limited, non-transferable right to use the purchased amount of the product
only to perform internal research for the sole benefit of the purchaser. No right to resell this product or any of its components
is conveyed expressly, by implication, or by estoppel. This product is for internal research purposes only and is not for use in
commercial applications of any kind, including, without limitation, quality control and commercial services such as reporting the
results of purchaser’s activities for a fee or other form of consideration. For information on obtaining additional righs, please contact
[e-mail] or Out Licensing, Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, California 92008.
Trademarks
The trademarks mentioned herein are the property of Life Technologies Corporation and/or its affiliate(s) or their respective owners.
© 2013 Life Technologies Corporation. All rights reserved.
Headquarters
5791 Van Allen Way | Carlsbad, CA 92008 USA | Phone [phone] | Toll Free in USA 800 955 6288
For support visit lifetechnologies.com/support or email [e-mail]
lifetechnologies.com
18 March 2013
USB
ports
Coaxial
focusing knob
Power
switch
Illumination
wheel
Objective
turret
Power
input jack
Phase turret
Freeze
button
Save
button
Mechanical Stage Controls*
* The attachable MechanicalStage
(Cat. no. AMEP 4712) is anoptional
feature. See the EVOS®XL Core
Imaging System User Guide
for installation and removal
instructions.
Stage
clip
Stage
Y-axis
knob
Stage
X-axis
knob

Overview
The EVOS®XL Core Imaging System has two
types of controls: manual and onscreen. Manual
controls include the illumination wheel, focus
knobs, Freeze and Save buttons, objective turret,
and the optional mechanical stage X-Y axis
knobs. Onscreen controls appear at the bottom
of the screen when the mouse rolls over the
controls area. Using a mouse is optional; you
can operate the EVOS®XL Core Imaging System
using only manual controls.
Note: The EVOS®XL Core Imaging SystemUser
Guide is provided on the USB flash drive. It isalso
available for downloading from the EVOS®XLCore
Imaging System product page at
www.lifetechnologies.com.
Operating Environment
Place the EVOS®XL Core Imaging System
on a level surface away from vibrations from
other pieces of equipment.
Allow at least 5 cm (2 in) of free space at
the back of the LCD housing to allow for
proper ventilation and prevent overheating
of electronic components.
Set up the instrument away from direct light
sources, such as windows. Ambient room
lighting can enter the imaging path and
affect the image.
Operating temperature range: 4°–32°C
(40°–90°F).
Relative humidity range: 30–90%. 2
Onscreen
Controls
Setup
1. Connect the power adapter to the power
jack on the back of the instrument base
and plug the cord into an outlet.
2. Plug the wireless mouse receiver into
either of theUSB ports located on the
right side of the LCD screen. Install the
battery and switch on the mouse.
3. Optional: If the instrument is equipped with
a mechanical stage, install the mechanical
stage following the instructions provided in
the user guide.
4. Unpack the glass stage plate and insert
it into the stage opening (if a mechanical
stage is installed, move it all the way back to
expose the stage opening).
5. Switch the instrument ON using the
power switch on the right side of the
instrument. Startup takes approximately
one minute. If the display remains black
after startup, adjust the illumination.
Note: For detailed setup instructions, refer
to the EVOS®XL Core Imaging SystemUser
Guide provided on the USB flash drive.
Basic Operation
1. Turn on the instrument using the power
switch on the right side of the base.
2. Plug a USB flash drive into one of the USB
ports on the right side of the instrument.
3. Place the sample on the glass stage
plate. If the instrument is equipped with
a mechanical stage, secure a vessel with
the stage clip to hold the sample over
the stage plate.
Note: Place slides with coverslips face up.
4. Set the objective turret to the desired
magnification.
5. Turn the phase turret to the position
that corresponds to the selected objective
and contrast method.
6. Bring the sample into focus using the
coaxial focus knob .
7. Adjust the illumination intensity if necessary,
using the onscreen illumination slider 
or the illumination wheel .
8. Use the onscreen Freeze/Live button 11
or the manual Freeze button 12 to acquire
the image.
9. Use the onscreen Save button 13 or the
manual Save button 14 to save the image.
10. Click the onscreen Freeze/Live button 11
or the manual Freeze button 12 to return
to live viewing.
Note: The Freeze/Live button toggles
between Freeze and Live.
34
Note: Onscreen controls are available only when
mouse is installed. Roll over bottom of screen with
mouse to activate controls.








14

12
Dimensions
The EVOS®XL Core Imaging System will fit in cell
culture hoods that are at least 18 inches (457mm)
deep. If your cell culture hood is smaller, it may be
necessary to turn the instrument at a slight angle
to fit.
DEPTH 16.0 in (40.6 cm)
WIDTH 12.5 in (31.8 cm)
HEIGHT, TRANSPORT 13.5 in (34.3 cm)
HEIGHT, DISPLAY 21.0 in (55.3 cm) 1311




Overview
The EVOS®XL Core Imaging System has two
types of controls: manual and onscreen. Manual
controls include the illumination wheel, focus
knobs, Freeze and Save buttons, objective turret,
and the optional mechanical stage X-Y axis
knobs. Onscreen controls appear at the bottom
of the screen when the mouse rolls over the
controls area. Using a mouse is optional; you
can operate the EVOS®XL Core Imaging System
using only manual controls.
Note: The EVOS®XL Core Imaging SystemUser
Guide is provided on the USB flash drive. It isalso
available for downloading from the EVOS®XLCore
Imaging System product page at
www.lifetechnologies.com.
Operating Environment
Place the EVOS®XL Core Imaging System
on a level surface away from vibrations from
other pieces of equipment.
Allow at least 5 cm (2 in) of free space at
the back of the LCD housing to allow for
proper ventilation and prevent overheating
of electronic components.
Set up the instrument away from direct light
sources, such as windows. Ambient room
lighting can enter the imaging path and
affect the image.
Operating temperature range: 4°–32°C
(40°–90°F).
Relative humidity range: 30–90%. 2
Onscreen
Controls
Setup
1. Connect the power adapter to the power
jack on the back of the instrument base
and plug the cord into an outlet.
2. Plug the wireless mouse receiver into
either of theUSB ports located on the
right side of the LCD screen. Install the
battery and switch on the mouse.
3. Optional: If the instrument is equipped with
a mechanical stage, install the mechanical
stage following the instructions provided in
the user guide.
4. Unpack the glass stage plate and insert
it into the stage opening (if a mechanical
stage is installed, move it all the way back to
expose the stage opening).
5. Switch the instrument ON using the
power switch on the right side of the
instrument. Startup takes approximately
one minute. If the display remains black
after startup, adjust the illumination.
Note: For detailed setup instructions, refer
to the EVOS®XL Core Imaging SystemUser
Guide provided on the USB flash drive.
Basic Operation
1. Turn on the instrument using the power
switch on the right side of the base.
2. Plug a USB flash drive into one of the USB
ports on the right side of the instrument.
3. Place the sample on the glass stage
plate. If the instrument is equipped with
a mechanical stage, secure a vessel with
the stage clip to hold the sample over
the stage plate.
Note: Place slides with coverslips face up.
4. Set the objective turret to the desired
magnification.
5. Turn the phase turret to the position
that corresponds to the selected objective
and contrast method.
6. Bring the sample into focus using the
coaxial focus knob .
7. Adjust the illumination intensity if necessary,
using the onscreen illumination slider 
or the illumination wheel .
8. Use the onscreen Freeze/Live button 11
or the manual Freeze button 12 to acquire
the image.
9. Use the onscreen Save button 13 or the
manual Save button 14 to save the image.
10. Click the onscreen Freeze/Live button 11
or the manual Freeze button 12 to return
to live viewing.
Note: The Freeze/Live button toggles
between Freeze and Live.
34
Note: Onscreen controls are available only when
mouse is installed. Roll over bottom of screen with
mouse to activate controls.








14

12
Dimensions
The EVOS®XL Core Imaging System will fit in cell
culture hoods that are at least 18 inches (457mm)
deep. If your cell culture hood is smaller, it may be
necessary to turn the instrument at a slight angle
to fit.
DEPTH 16.0 in (40.6 cm)
WIDTH 12.5 in (31.8 cm)
HEIGHT, TRANSPORT 13.5 in (34.3 cm)
HEIGHT, DISPLAY 21.0 in (55.3 cm) 1311




Overview
The EVOS®XL Core Imaging System has two
types of controls: manual and onscreen. Manual
controls include the illumination wheel, focus
knobs, Freeze and Save buttons, objective turret,
and the optional mechanical stage X-Y axis
knobs. Onscreen controls appear at the bottom
of the screen when the mouse rolls over the
controls area. Using a mouse is optional; you
can operate the EVOS®XL Core Imaging System
using only manual controls.
Note: The EVOS®XL Core Imaging SystemUser
Guide is provided on the USB flash drive. It isalso
available for downloading from the EVOS®XLCore
Imaging System product page at
www.lifetechnologies.com.
Operating Environment
Place the EVOS®XL Core Imaging System
on a level surface away from vibrations from
other pieces of equipment.
Allow at least 5 cm (2 in) of free space at
the back of the LCD housing to allow for
proper ventilation and prevent overheating
of electronic components.
Set up the instrument away from direct light
sources, such as windows. Ambient room
lighting can enter the imaging path and
affect the image.
Operating temperature range: 4°–32°C
(40°–90°F).
Relative humidity range: 30–90%. 2
Onscreen
Controls
Setup
1. Connect the power adapter to the power
jack on the back of the instrument base
and plug the cord into an outlet.
2. Plug the wireless mouse receiver into
either of theUSB ports located on the
right side of the LCD screen. Install the
battery and switch on the mouse.
3. Optional: If the instrument is equipped with
a mechanical stage, install the mechanical
stage following the instructions provided in
the user guide.
4. Unpack the glass stage plate and insert
it into the stage opening (if a mechanical
stage is installed, move it all the way back to
expose the stage opening).
5. Switch the instrument ON using the
power switch on the right side of the
instrument. Startup takes approximately
one minute. If the display remains black
after startup, adjust the illumination.
Note: For detailed setup instructions, refer
to the EVOS®XL Core Imaging SystemUser
Guide provided on the USB flash drive.
Basic Operation
1. Turn on the instrument using the power
switch on the right side of the base.
2. Plug a USB flash drive into one of the USB
ports on the right side of the instrument.
3. Place the sample on the glass stage
plate. If the instrument is equipped with
a mechanical stage, secure a vessel with
the stage clip to hold the sample over
the stage plate.
Note: Place slides with coverslips face up.
4. Set the objective turret to the desired
magnification.
5. Turn the phase turret to the position
that corresponds to the selected objective
and contrast method.
6. Bring the sample into focus using the
coaxial focus knob .
7. Adjust the illumination intensity if necessary,
using the onscreen illumination slider 
or the illumination wheel .
8. Use the onscreen Freeze/Live button 11
or the manual Freeze button 12 to acquire
the image.
9. Use the onscreen Save button 13 or the
manual Save button 14 to save the image.
10. Click the onscreen Freeze/Live button 11
or the manual Freeze button 12 to return
to live viewing.
Note: The Freeze/Live button toggles
between Freeze and Live.
34
Note: Onscreen controls are available only when
mouse is installed. Roll over bottom of screen with
mouse to activate controls.








14

12
Dimensions
The EVOS®XL Core Imaging System will fit in cell
culture hoods that are at least 18 inches (457mm)
deep. If your cell culture hood is smaller, it may be
necessary to turn the instrument at a slight angle
to fit.
DEPTH 16.0 in (40.6 cm)
WIDTH 12.5 in (31.8 cm)
HEIGHT, TRANSPORT 13.5 in (34.3 cm)
HEIGHT, DISPLAY 21.0 in (55.3 cm) 1311




5
EVOS®XL Core
Imaging System
for Transmitted Light Applications
Catalog Number AMEX1000
Doc. Part no. ZP-PKGA-0583
Pub. no. MAN0007718
Rev. 1.0
For Research Use Only. Not for use in diagnostic procedures.
QUICK REFERENCE
Limited Product Warranty
Life Technologies Corporation and/or its affiliate(s) warrant their products as set forth in the Life Technologies’ General Terms and
Conditions of Sale found on Life Technologies’ website at www.lifetechnologies.com/termsandconditions. If you have any questions,
please contact Life Technologies at www.lifetechnologies.com/support.
DISCLAIMER
LIFE TECHNOLOGIES CORPORATION AND/OR ITS AFFILIATE(S) DISCLAIM ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT,
EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY, FITNESS FOR A PARTICULAR
PURPOSE, OR NON-INFRINGEMENT. TO THE EXTENT ALLOWED BY LAW, IN NO EVENT SHALL LIFE TECHNOLOGIES AND/OR
ITS AFFILIATE(S) BE LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER BASIS
FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING
FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE USE THEREOF.
Limited Use Label License: Research Use Only
The purchase of this product conveys to the purchaser the limited, non-transferable right to use the purchased amount of the product
only to perform internal research for the sole benefit of the purchaser. No right to resell this product or any of its components
is conveyed expressly, by implication, or by estoppel. This product is for internal research purposes only and is not for use in
commercial applications of any kind, including, without limitation, quality control and commercial services such as reporting the
results of purchaser’s activities for a fee or other form of consideration. For information on obtaining additional righs, please contact
[e-mail] or Out Licensing, Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, California 92008.
Trademarks
The trademarks mentioned herein are the property of Life Technologies Corporation and/or its affiliate(s) or their respective owners.
© 2013 Life Technologies Corporation. All rights reserved.
Headquarters
5791 Van Allen Way | Carlsbad, CA 92008 USA | Phone [phone] | Toll Free in USA 800 955 6288
For support visit lifetechnologies.com/support or email [e-mail]
lifetechnologies.com
18 March 2013
USB
ports
Coaxial
focusing knob
Power
switch
Illumination
wheel
Objective
turret
Power
input jack
Phase turret
Freeze
button
Save
button
Mechanical Stage Controls*
* The attachable MechanicalStage
(Cat. no. AMEP 4712) is anoptional
feature. See the EVOS®XL Core
Imaging System User Guide
for installation and removal
instructions.
Stage
clip
Stage
Y-axis
knob
Stage
X-axis
knob

5
EVOS®XL Core
Imaging System
for Transmitted Light Applications
Catalog Number AMEX1000
Doc. Part no. ZP-PKGA-0583
Pub. no. MAN0007718
Rev. 1.0
For Research Use Only. Not for use in diagnostic procedures.
QUICK REFERENCE
Limited Product Warranty
Life Technologies Corporation and/or its affiliate(s) warrant their products as set forth in the Life Technologies’ General Terms and
Conditions of Sale found on Life Technologies’ website at www.lifetechnologies.com/termsandconditions. If you have any questions,
please contact Life Technologies at www.lifetechnologies.com/support.
DISCLAIMER
LIFE TECHNOLOGIES CORPORATION AND/OR ITS AFFILIATE(S) DISCLAIM ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT,
EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY, FITNESS FOR A PARTICULAR
PURPOSE, OR NON-INFRINGEMENT. TO THE EXTENT ALLOWED BY LAW, IN NO EVENT SHALL LIFE TECHNOLOGIES AND/OR
ITS AFFILIATE(S) BE LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER BASIS
FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING
FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE USE THEREOF.
Limited Use Label License: Research Use Only
The purchase of this product conveys to the purchaser the limited, non-transferable right to use the purchased amount of the product
only to perform internal research for the sole benefit of the purchaser. No right to resell this product or any of its components
is conveyed expressly, by implication, or by estoppel. This product is for internal research purposes only and is not for use in
commercial applications of any kind, including, without limitation, quality control and commercial services such as reporting the
results of purchaser’s activities for a fee or other form of consideration. For information on obtaining additional righs, please contact
[email protected]om or Out Licensing, Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, California 92008.
Trademarks
The trademarks mentioned herein are the property of Life Technologies Corporation and/or its affiliate(s) or their respective owners.
© 2013 Life Technologies Corporation. All rights reserved.
Headquarters
5791 Van Allen Way | Carlsbad, CA 92008 USA | Phone [phone] | Toll Free in USA 800 955 6288
For support visit lifetechnologies.com/support or email [e-mail]
lifetechnologies.com
18 March 2013
USB
ports
Coaxial
focusing knob
Power
switch
Illumination
wheel
Objective
turret
Power
input jack
Phase turret
Freeze
button
Save
button
Mechanical Stage Controls*
* The attachable MechanicalStage
(Cat. no. AMEP 4712) is anoptional
feature. See the EVOS®XL Core
Imaging System User Guide
for installation and removal
instructions.
Stage
clip
Stage
Y-axis
knob
Stage
X-axis
knob