Lonza Amaxa HT Nucleofector User manual

BioResearch
Amaxa™ HT Nucleofector™ hardware manual
For research use only

The purchase of the HT Nucleofector™ conveys to the buyer
the non-transferable right to use the system as well as
Lonza’s proprietary Nucleofector™ technology only for
research conducted by the buyer (whether the buyer is an
academic or for-prot entity). For further details about the
license please refer to chapter 5.0.

Content
1 The Amaxa™ Nucleofector™ technology 4
2 Operating instructions 6
2.1 Restrictions 6
2.2 Maintenance 6
2.3 Safety instructions – read carefully 7
2.4 Waste disposal 8
2.5 HT Nucleofector™ system components 8
2.6 System set-up instructions 10
2.7 Instructions for using the HT Nucleofector™ system 11
3 Running an experiment 12
3.1 Processing a 384-well Nucleocuvette™ plate 12
3.2 System shutdown 12
4 Troubleshooting 13
4.1 Nucleofection™ problems 13
4.2 Mechanical failures 14
5 Purchaser notication 15
6 Appendix 16
6.1 Technical data power supply 16
6.2 Technical data plate handler 16

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HT Nucleofector™ hardware manual
1 The Amaxa™ Nucleofector™ technology
Since its introduction in 2001, Amaxa™ Nucleofector™ technology has
transformed transfection. Nucleofection™ has proven to be a reliable and
reproducible easy-to-use technology that is suited to a wide variety of
applications and provides an answer to almost every transfection
challenge. An ever-increasing number of publications in a wide range of
research areas reect how Nucleofector™ technology is driving research
in numerous cell types and applications.
How it works
Nucleofector™ technology is based on two unique components: the
Nucleofector™ system, which delivers the specically optimized electrical
parameters and Nucleofector™ kits, which contain specic Nucleofector™
solutions. As part of Nucleofector™ technology, Lonza provides cell-type
specic optimized protocols for many dierent cell lines and primary cells.
Transfection of any cell
With over 160 protocols for cell lines and primary cells optimized by
Lonza’s R&D team and more than 680 entries in the online cell data base,
Nucleofector™ technology is clearly the transfection method-of-choice
for any dicult-to-transfect cell type. Moreover, Nucleofection™ is the
only electroporation based technology allowing reliable transfection of
adherent cells and cells in suspension.
Transfection of any substrate
Nucleofector™ technology oers high exibility within applications,
since the same transfection parameters apply for almost all substrates.
DNA vectors, RNA duplexes, and peptides can be transfected using our
sophisticated transfection protocols. This makes Nucleofection™ the
ideal tool for providing answers to scientic questions in over expression
studies, gene silencing approaches, protein expression, generation of
stable clones and many more applications.
www.lonza.com /cell-database

5
The HT Nucleofector™ system
The HT Nucleofector™ — designed for high-throughput approaches
The HT Nucleofector™ system was developed for ecient, reproducible,
and exible high-throughput transfection. With up to 95% transfection
eciency and the ability to deliver various substrates into virtually any cell
type, the HT Nucleofector™ system meets your screening requirements.
Experience the benets of the HT Nucleofector™ system:
Automation-compatible – full integration into liquid handling platforms
Fast – process 384 samples in one minute
Screening-compatible – low cell number required per sample
For ne-tuning of the optimization process, please contact our
scientic support teams
Europe
Phone: + 49 221 99199 400
E-mail: scienti[e-mail]
North America
Phone: [phone] (toll free)
E-mail: scienti[e-mail]
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HT Nucleofector™ hardware manual
2.1 Restrictions
Medical use restrictions
Nucleofector™ technology is intended for research and investigational
use by professionals only. Note that the Nucleofector™ technology is not
intended to be used for diagnostic purposes, or for testing or treatment
in humans.
License statement
Lonza is holder of various patents, patent applications, copyrights
and technical and scientic experience with respect to Nucleofector™
technology. Use of Lonza’s Nucleofector™ technology and/or related
software requires a license from Lonza.
The purchase of the HT Nucleofector™ conveys to the buyer the
non-transferable right to use the system as well as Lonza’s proprietary
Nucleofection™ technology only for research conducted by the buyer
(whether the buyer is an academic or for-prot entity). For further details
about the license, refer to chapter 5.
For license information contact Lonza Cologne GmbH:
Phone: + 49 221 99199 0
E-mail: [e-mail]
2.2 Maintenance
The HT Nucleofector™ system requires minimal maintenance for reliable
operation.
To clean the plate handler, switch o the power supply and use a
damp cloth to clean the outer casing with water or 70% ethanol.
NOTE: Avoid wetting the 384-well Nucleocuvette™ plate carousel, especially the surface of
the retainer and the connectors located on the left side of the device.
To remove dust from the HT Nucleofector™ power supply, switch
off the device and remove the power cable from the power inlet.
Use a dry cloth to remove dust from the surface of the power supply.
NOTE: Do not use any liquid for cleaning the power supply. Liquids entering the unit may
cause severe damage.
2 Operating instructions

7
2.3 Safety instructions – please read carefully
This symbol means that there is a risk of electric shock.
An electric shock could cause death or personal injury.
The HT Nucleofector™ system has been certified by international
safety standards and is safe to use when operated in accordance with
this manual.
Only use the HT Nucleofector™ power supply in connection with the
HT Nucleofector™ plate handler. Under no circumstances should it be
connected to any other device delivering high-voltage electrical impulses.
The system is designed to deliver variable high-voltage impulses for the
purpose of introducing substrates into eukaryotic cells.
These electrical impulses can be deadly.
Therefore, use this system with care and take the following
precautions:
– Only use the system once you have read and understood the
HT Nucleofector™ manual. The manual should be accessible to all users.
Make sure that each potential user reads and understands it.
– Set up the system in a dry place. Avoid spilling liquid onto or into the
HT Nucleofector™ power supply or the HT Nucleofector™ plate handler.
Do not use the system if any components are wet.
– Place the HT Nucleofector™ system components on top of a safe, level
and stable table or bench.
– Before connecting/disconnecting any of the interface cables between
the HT Nucleofector™ plate handler and the HT Nucleofector™ power
supply, ensure that the HT Nucleofector™ power supply is switched
o and the power cable is removed from the power inlet.
– Prevent sudden impacts and vibration while moving and transporting
system components.
– Only use HT Nucleofector™ devices in conjunction with the HT
Nucleofector™ software.
– Standard Nucleofector™ solutions and the Nucleofector™ solutions
used for the HT Nucleofector™ are not compatible.
– Do not use solutions or plates from any source other than Lonza.
– Unpack the 384-well Nucleocuvette™ plates immediately before the
experiment. Make sure that the outer contact areas are dry.
– Always place the lid onto the 384-well Nucleocuvette™ plate before
transferring it to the HT Nucleofector™ plate handler.
– Safety may be compromised if any uid has been spilled in the close
vicinity of the HT Nucleofector™ system. Ensure that no uid comes
into contact with or enters the system components. If any uid has
been spilled in the close vicinity of or onto the HT Nucleofector™ plate
system, contact Lonza scientic support for advice on precautions to
be taken before further use.
– Do not open the device housing. Under no circumstances should circuit
components be interfered with, as they can deliver an electric shock
even when the system is not in operation.
– Do not alter the device in any manner.
– Do not use the device if the insulation of the high voltage cable
connecting the plate handler and the power supply is damaged.
– Do not use the device if any of the housing parts or covers are missing.
– Do not detach any of the housing parts or covers.
– Do not expose the device to a humid environment.
– Do not expose the device to direct sunlight or place the instrument
in a hot environment.
– Under no circumstances plug the high-voltage cables of the
HT Nucleofector™ plate handler into external electrode sockets of
any device other than the HT Nucleofector™ power supply. Use of a
device from any source other than Lonza will invalidate all warranty
and liability claims.
– Do not use lids other than those provided with the 384-well
Nucleofector™ kits as the system is calibrated to the supplied lids.
Other lids may cause damage to the system.
– Do not obstruct any moving parts, e.g. the plate carousel of the
HT Nucleofector™ plate handler.
– Do not move the HT Nucleofector™ plate handler if a 384-well
Nucleocuvette™ plate is inside the device. Moving the plate handler
may cause sample uid from the plate to spill into the device.
– Never place any foreign object onto the device or onto the plate
carousel. If a foreign object has entered the HT Nucleofector™ plate
handler, the safety of the device may be compromised. Contact
Lonza scientic support for advice on precautions to be taken before
further use.
– The device is not approved for use in re- or explosion-endangered
areas, or for use with inammable or explosive media.
– If any component of the HT Nucleofector™ system has been damaged,
ensure that the HT Nucleofector™ system cannot be used (e.g. by
disconnecting the HT Nucleofector™ power supply), and contact Lonza
scientic support for assistance.
– Service shall only be performed by personnel authorized by Lonza.
– Handling of device parts that may be contaminated by samples shall
always be performed with protective gloves and any disposal of such
parts must be according to federal, state or local procedures for clinical
waste handling and disposal. Use secure leak-proof containers and
avoid unprotected handling of such parts.
Note: Lonza disclaims all warranties expressed or implied and shall in no event be liable
for any kind of damages caused by or arising out of any operation or use in violation of the
safety and handling instructions above.
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Figure 2.2: HT Nucleofector™ set of cables
3. Power cable
4. Lonza interface data cable
5. Lonza interface HV cable
6. USB cable
2.4 Waste disposal
Disposal of consumables from HT Nucleofector™ kits (cuvettes, and
Nucleofector™ solutions): dispose of cuvettes and expired 384-well
Nucleofector™ solutions in a biohazard container. Refer to your local
waste management organization and to the relevant laboratory safety
instructions for proper disposal practices.
2.5 HT Nucleofector™ system components
The HT Nucleofector™ system comprises two hardware modules (the power
supply and plate handler), HT Nucleofector™ software preinstalled on a
Windows®-based laptop, and a set of cables to connect the units.
Figure 2.1: HT Nucleofector system components
1. HT Nucleofector™ power supply
2. HT Nucleofector™ plate handler
1 2
4 5
6 3

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HT Nucleofector™ power supply
The HT Nucleofector™ power supply generates and delivers the electrical
programs to the plate handler and mediates the data transfer between
the computer and the plate handler. It is connected to the plate handler
via two cables.
7. Main switch with status LED
8. Main power inlet
9. Fuse holders with fuses
10. Lonza interface (HV port)
11. Lonza interface (data port)
12. Ethernet port
Figure 2.3: HT Nucleofector™ power supply
7
810 9
12 11
HT Nucleofector™ plate handler
The HT Nucleofector™ plate handler serves as the contacting unit of the
system. Transfection of the samples occurs in 384-well Nucleocuvette™
plates that are loaded onto the carousel of the plate handler.
13. Lonza interface (HV port)
14. USB host (for rmware update)
15. USB port for PC connection
16. Ethernet port
17. Lonza interface (data port)
Figure 2.4: Plate handler connections
Plate handler front
18. Status LED from the left: power on (green); boot status (green);
error (red); plate status (blue)
19. Plate carousel
20. Plate retainer position
Figure 2.5: Plate handler front view
13 17
15
14 16
18 2019
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The Nucleofector™ software start screen
The HT Nucleofector™ operating software provides the graphical user
interface for operating the HT Nucleofector™ system. It controls all
processes during an experiment and manages data transfer to the host PC.
Figure 2.6: Login screen
384-well Nucleocuvette™ plate – solution and supplement
High-throughput Nucleofection™ occurs in unique 384-well Nucleocuvette™
plates supplied with HT Nucleofector™ kits. The plates meet SBS standards
and can therefore be used in liquid handling workstations.
Figure 2.7: HT Nucleofector consumables
2.6 System set-up instructions
The HT Nucleofector™ system will be installed and checked by a Lonza
technical specialist. Follow the instructions below if the instrument is
moved from its original location.
– Connect the HV port of the HT Nucleofector™ power supply (10) to the
HV port on the plate handler Unit (13) using the cable marked “Lonza
interface HV cable.”
– Connect the data port of the HT Nucleofector™ power supply (11) to
the data port on the plate handler (17) using the cable marked “Lonza
interface data cable.”
– Connect the USB slave port of the HT Nucleofector™ plate handler (15)
to a USB port on your personal computer using the supplied USB cable.
– If required install, the HT Nucleofector™ operating software on
your personal computer, following the instructions in the HT
Nucleofector™ software manual.
– Connect the main power plug of the HT Nucleofector™ power supply
(8) to a wall outlet with protective grounding conforming to the power
rating specied in Chapter 6.
– Start the system as described in Chapter 3.1 of the HT Nucleofector™
software manual.

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2.7 Instructions for using the
HT Nucleofector™ system
The table below gives a brief overview of the steps required for a typical
Nucleofection™ workow.
Step Description For more information see
1Switch on the HT Nucleofector™ system. This chapter
2Start the HT Nucleofector™ software on the computer and log in. Software manual
3Check your user settings (optional). Software manual
4Check that all connections are active / reconnected Software manual
5Open an existing experiment or create a new experiment. Software manual
6Prepare and transfer samples into the 384-well Nucleocuvette™ plate. Optimized protocol
7Insert the 384-well Nucleocuvette™ plate into the HT Nucleofector™ plate handler. Chapter 3.1
8Start Nucleofection™ process from the computer. Software manual
9Monitor Nucleofection™ process during program execution (optional). Software manual
10 Remove 384-well Nucleocuvette™ plate from the plate handler and transfer samples to
appropriate culture plates.
Optimized protocol
11 Check result le on the computer for potential errors. Software manual
12 Close les, exit HT Nucleofector™ software and switch o all components. Software manual
The HT Nucleofector™ system enables Nucleofection™ experiments to be
conveniently controlled and monitored from a computer. Only a few steps
are required to initialize the system.
– Switch on the HT Nucleofector™ system via the main switch located
on the front panel of the power supply. LED 1 (green) on the plate
handler front panel is lit.
– Start the HT Nucleofector™ software on the personal computer and
log in. LED 2 (green) on the plate handler front panel blinks during
the boot sequence and remains lit when the process is successfully
completed. The system is now initialized.
– If LED 3 (red) is lit, an error occurred during the boot process. Refer to
the HT Nucleofector™ software manual for troubleshooting.
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3 Running an experiment
This chapter explains how to run a Nucleofection™ experiment on the
HT Nucleofector™ system. Because correct cell handling before and
after the Nucleofection™ process is crucial for successful experiments,
refer to the dedicated optimized protocol to nd detailed guidelines for
these steps.
3.1 Processing a 384-well
Nucleocuvette™ plate
This chapter describes loading and processing of the 384-well
Nucleocuvette™ plate using a predefined experiment. See the
HT Nucleofector™ software manual for further details on dening and
modifying experiments.
Figure 3.1: 384-well Nucleocuvette™ plate handler work area
The plate handler carousel has two plate retainer positions for
384-well Nucleocuvette™ plates. Load plates into the retainer position
that is accessible at the front of the plate handler. Do not turn the plate
handler carousel manually to access the second position. To load
prepared plates:
– Make sure that the plate is covered with an appropriate lid.
– Make sure that plate position A01 is in the upper left corner.
– Place the plate gently into the plate handler retainer position (1)
After loading the 384-well Nucleocuvette™ plate, start the experiment from
the computer. The 384-well Nucleocuvette™ plate will be automatically
rotated into the plate handler to be processed. It will be rotated back when
the experiment is completed. You can follow the progress of the experiment
by checking the blue progress LEDs on the front panel of the plate handler.
If the HT Nucleofector™ is integrated into an automated liquid handling
system follow the appropriate instructions in the HT Nucleofector™
software manual for plate handling.
NOTE: Do not attempt to turn the carousel manually when the system is active! This will
compromise system integrity.
NOTE: Do not use lids other than those provided in the 384-well Nucleofector™ kits. Other
lids may cause damage to the HT Nucleofector™ plate handler.
After completion of a run, remove the processed 384-well Nucleocuvette™
plate from the plate handler and process it according to the relevant
optimized protocol.
3.2 System shutdown
Close all parameter and result les, exit the HT Nucleofector™ operating
software, and switch o the system using the main switch on the
HT Nucleofector™ power supply front panel.
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4 Troubleshooting
The following troubleshooting guide may be helpful if experiments using
the HT Nucleofector™ system do not provide the expected results. The
comments are intended to help optimize experimental conditions.
Should you have any questions regarding the HT Nucleofector™ device,
please do not hesitate to contact Lonza’s scientic support team
Europe
Phone: + 49 221 99199 400
Fax: [phone]
scienti[e-mail]
www.lonza.com/nucleofection-support
North America
Phone: [phone] (toll free)
Fax: [phone]
scienti[e-mail]
www.lonza.com/nucleofection-support
4.1 Nucleofection™ problems
Problem Possible cause Solution
Low survival rate Cells remained too long in the
Nucleocuvette™ plate
If a 10 minute post incubation step is not recommended for your specic protocol, add pre-warmed medium to
the cells and remove samples immediately after the Nucleocuvette™ plate has been processed.
Cells are damaged by harvesting or
through inappropriate handling
Avoid severe conditions during harvesting, especially centrifugation at high speeds and overexposure to trypsin.
Pipette cells smoothly. After Nucleofection™, add the appropriate amount of pre-warmed medium to the wells and
resuspend cells gently before removing them.
Cells are stressed by culture
conditions
Cells should be viable and have been in culture for a certain number of passages. Freshly thawed cells should not
be used for Nucleofection™.
Avoid high cell densities or conuencies since this may negatively inuence the viability of the cells after
Nucleofection™. For detailed recommendations on passage number, cell density and conuency refer to the
relevant optimized protocol.
Low transfection
ecieny
Cells are stressed by centrifugation Centrifuge at lower speed (max. 90 x g).
Multiple use of 384-well
Nucleocuvette™ plates
We strongly recommend using 384-well Nucleocuvette™ plates only once, because the electric pulses that
are applied impair their physical integrity. The performance of plates that are used more than once will be
dramatically reduced .
Cells may be contaminated with
mycoplasmas
Test cultures for mycoplasma contamination. Contact our scientic support team for further advice.
Poor DNA quality Use high-purity plasmid DNA. We strongly recommend the use of high-quality products for plasmid purication
such as the QIAGEN EndoFree® plasmid kit. Do not use procedures involving phenol or chloroform treatment for
DNA purication.
Plasmid amount is too low We recommend a plasmid amount between 0.2–1 µg DNA per sample. If both gene transfer eciency and cell
mortality are low, the plasmid amount can be increased up to 2 µg per sample. Increasing the DNA amount may
lead to higher gene transfer eciencies but at the same time may result in higher cell mortality.
High cell conuency or density In many cell types, gene transfer eciency is poor if the cell density has been too high at the time of harvest.
Follow the guidelines in the relevant optimized protocol.
Cell number too high or too low We recommend using 2 × 104– 1 × 106cells per sample for cell lines and primary cells. Refer to the relevant
optimized protocol for specic details.
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Figure 4.1: Plastic cap covering the emergency release of the contact bridge
Figure 4.2: Lift contact bridge by turning the screw clockwise
Figure 4.3: Turn carousel manually to recover samples
4.2 Mechanical failures
Great care has been taken to ensure that mechanical failures will not occur
as long as the system is operated using the guidelines presented in this
manual and in the HT Nucleofector™ software manual.
In case of a system failure while a 384-well Nucleocuvette™ plate is still
inside the plate handler unit, proceed as follows to recover your samples:
1. Turn o the HT Nucleofector™ using the main switch of the
power supply.
2. Disconnect the main power cable from the power supply power
inlet.
3. Remove the small plastic cap on top of the plate handler housing
(see Figure 4.1) to access the emergency lift screw of the contact
bridge.
4. Using a screwdriver, turn the screw clockwise as far as possible.
5. The plate handler carousel can be turned manually and samples can
be recovered.
6. To recover a 384-well Nucleocuvette™ plate loaded on plate retainer
position 1 (position 2 is visible at the front of the plate handler)
turn the carousel counterclockwise to recover your samples. To
recover samples from plate retainer position 2 (position 1 is
visible), turn the carousel clockwise.
7. Remove the 384-well Nucleocuvette™ plate from the plate handler
carousel.
8. Contact Lonza’s technical service for analyzing the error and for
repair of the system.

15
The purchase of the HT Nucleofector™ conveys to the buyer a non-
transferable, non-exclusive license without the right to grant
sublicenses, limited for solely the use of Lonza Cologne’s proprietary
Nucleofector™ process and method.
The license is limited to research use only. The license does not include the
right to use the purchased device, its components or materials for any type
whatsoever of commercial purpose as dened below. Notwithstanding
the foregoing, the buyer shall be allowed to use the licensed process in
combination with the purchased device to perform services internally for
aliates, subsidiaries, sections, departments or scientic collaborators,
provided that such services are not for any commercial purpose.
Under no circumstances shall the licensed process and/or the purchased
device be used for testing in or treatment of humans. The buyer
acknowledges that the licensed process and/or the purchased device are
not diagnostic or therapeutic tools and that they shall not be used as an
accessory or complement to such tools.
Commercial purposes means any activity by a party for a nancial
consideration and may include, but is not limited to: (1) use of the
product or its components in manufacturing; (2) use of the product or
its components to provide a service, information, or data; (3) use of the
product or its components for therapeutic, diagnostic or prophylactic
purposes; or (4) resale of the product or its components, whether or not
such product or its components are resold for use in research.
The buyer may not manufacture, copy, reproduce, transmit, distribute, sell,
lease, transfer or sublicense the licensed process and/or the purchased
device.
For information on purchasing a license to this product for purposes
other than research, contact our licensing department:
Phone: + 49 221 99199 0
E-mail: [e-mail]
Representations and warranties
Lonza Cologne represents and warrants that, it is not aware of
any legal deciencies of the patents and that to its knowledge it has the
right to dispose of the patents. Lonza Cologne further represents and
warrants that it has no knowledge of any rights of third parties which have
priority over the patents and that the inventors of the invention falling
under the patents have transferred all rights and entitlements to Lonza
Cologne and that accordingly Lonza Cologne has full power and authority
to grant the license provided hereunder.
Lonza Cologne does not warrant that the information and data pertaining
to the licensed process and/or the purchased device is correct and without
defects, that the use of such information or data is adequate for the use
of the licensed process and/or the purchased device or that the technical
information or data for the licensed process and/or the purchased device
is complete.
Lonza Cologne does not warrant either that the use of the license does not
infringe third parties’ rights or does not cause damages to third parties.
Any liability for later invalidation or lapse of the patents is excluded.
Subject to the warranty granted for the purchased device, all warranty
claims are excluded as far as they are based on the licensed process
or the purchased device. No warranty is made for the reliability, the
quality, the economic utilization, eciency, merchantability, tness
for purpose and the usefulness of the licensed process, the purchased
device, or products resulting from the use of the licensed process or the
purchased device, for the intended purpose or for any other purpose. The
exclusion of warranty claims does not apply in case of gross negligence
or intentional misconduct of Lonza Cologne.
Lonza Cologne does not assume any liability that the licensed process,
the purchased device or any product resulting from the use of the licensed
product shall be approved by governmental authorities or any other
institution or supervisory board or similar agencies.
5 Purchaser notication
5

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Technical data power supply
Power supply 100–110 VAC or 230 VAC
50–60 Hz
Self-regulating
Attention:
Only use power outlets with protective earth/ground
Only use the power cable supplied with the HT Nucleofector™ system
Power consumption 750 VA / fuse T10A H250VP
Operation temperature range +15°C to +40°C, non-condensing
Safety class EN 61010-1
UL 61010-1
IP 20
Weight 14 kg
31 lb
Dimensions (w × d × h) 13.5 × 50 × 45 cm
5.3× 19.6 × 17.7 in
Manufacturing date The year of manufacture is indicated by the second and third digit of the serial number, e.g. a device with the serial number x11xxxxx was
manufactured in 2011.
Technical data plate handler
Power supply 24 V supplied by HT Nucleofector™ power supply
Operation temperature range +15°C to +40°C, non-condensing
Safety class EN 61010-1
UL 61010-1
IP 20
Weight 10.5 kg
23.1 lb
Dimensions (w × d × h) 40 × 40 × 15 cm
15.7 × 15.7 × 5.9 in
Manufacturing date The year of manufacture is indicated by the second and third digit of the serial number, e.g. a device with the serial number x11xxxxx was
manufactured in 2011.
6 Appendix

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Notes
6

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Notes

19
Notes

www.lonza.com
www.lonza.com /specications
Lonza Cologne GmbH – 50829 Cologne, Germany
For research use only. Not for use in diagnostic procedures.
Manufacturer and distributor information: HT Nucleofector™ system
is manufactured by Lonza Cologne GmbH, Nattermannallee 1, 50829
Cologne, Germany and distributed in the US by Lonza Walkersville, Inc.
(8830 Biggs Ford Road, Walkersville, MD 21793).
The use of this product, alone or in combination with materials and/or
methods of others, may require a license from a third party. User shall
be fully responsible for determining whether and from whom it requires
such license and for obtaining such license.
The Amaxa™ Nucleofector™ technology, comprising Nucleofection™
process, Nucleofector™ device, Nucleofector™ solutions, Nucleofector™
96-well Shuttle™ system and Nucleocuvette™ plates and modules
is covered by patent and/or patent pending rights owned by Lonza
Cologne GmbH.
Amaxa, Nucleofector, Nucleofection, Nucleocuvette, maxGFP and 96-well
Shuttle are trademarks of Lonza Cologne GmbH in Germany and/ or the
U.S. and / or other countries.
Other product and company names mentioned herein are the trademarks
of their respective owners.
Amaxa™ Nucleofector™ kits contain a proprietary nucleic acid coding for a
proprietary copepod protein uorescent protein intended to be used as
a positive control with Lonza products only. Any use of the proprietary
nucleic acid or protein other than as a positive control with a Lonza
product is strictly prohibited. Use in any other application requires a
license from Evrogen. To obtain such a license, please contact Evrogen
at [e-mail].
The information contained herein is believed to be correct and
corresponds to the latest state of scientic and technical knowledge.
However, no warranty is made, either expressed or implied, regarding its
accuracy or the results to be obtained from the use of such information
and no warranty is expressed or implied concerning the use of these
products. The buyer assumes all risks of use and/or handling. No
statement is intended or should be construed as a recommendation to
infringe any existing patent.
© Copyright 2011, Lonza Cologne GmbH. All rights reserved.
CD-MN030
Contact information
Online ordering
https://shop.lonza.com
Customer service
North America
800 638 8174 (toll free)
Europe
+ 32 87 321 611
International
Contact your local Lonza distributor
[phone], ext. 1500
Scientic support
North America
800 521 0390 (toll-free)
scienti[e-mail]
Europe
[phone]
scienti[e-mail]
International
scienti[e-mail]
International oces
Australia [phone]
Austria 0800 201 538 (toll free)
Belgium [phone]
Brazil [phone]
Denmark 808 83 159 (toll free)
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India [phone]
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