MAPADA V-1600 User manual

V-1600
USER’S MANUAL
DOMINIQUE DUTSCHER SAS

1600/1800 Manual
1
Co
oo
ontents
Safety ….…………………………………………………………………………. 1
11
1
General ……………….…………………………………………………………. 1
Electrical ……………………………………………………………………….. 1
Warning ………………………………………………………………………… 1
Working Principle …………………………………………………………… 2
Unpacking Instructions …………………………………….………………… 2
Specifications ……………………………………………..……………… 2
Installation ………………………………………………………..…………… 3
Operation ………………………………………………………..……………… 4
Prepare the Spectrophotometer ………………………………………… 4
Description of keys ………… …………………………………… 5
Turn on spectrophotometer ……………………………………………… 5
Basic operation…………………………………..……………………………..6
Analyze Sample ……………………………………………………………...……8
Basic Mode ……………………..……………………………………………….8
Quantitative ……………………………………………………………………..9
Kinetics …….……………………………………………………………………16
Utility ………………………………………………………………………… 21
Maintenance……………………………………………………………………. 27
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Safety:
The safety statements in this manual comply with the requirements of the HEALTH AND
SAFETY AT WORK ACT, 1974.
Read the following before installing and using the instrument and its accessories. The
MAPADA UV/V-1600/1800 should be operated by appropriate laboratory technicians.
General:
The apparatus described in this manual is designed to be used by properly trained
personnel in a suitable equipped laboratory. For the correct and safe use of this
apparatus it is essential that laboratory personnel follow generally accepted safe
procedures in addition to the safety precautions called for in this manual.
The covers on this instrument may be removed for servicing. However, the inside of the
power supply unit is a hazardous area and its cover should not be removed under any
circumstances. There are no serviceable components inside this power supply unit.
For MAPADA UV/V-1600/1800, avoid touching the high voltage power supply at all times.
Some of the chemicals used in spectrophotometer are corrosive and/or inflammable and
samples may be radioactive, toxic, or potentially infective. Care should be taken to
follow the normal laboratory procedures for handling chemicals and samples.
Electrical:
Before switching on the apparatus, make sure it is set to the voltage of the local power
supply (see Fig.1-1).
Fig. 1-1
The power cord shall be inserted in a socket provided with a protective earth contact.
The protective action must not be negated by the use of an extension cord without a
protective conductor.
Warning:
Any interruption of the protective conductor inside or outside the apparatus or
disconnection of the protective earth terminal is likely to make the apparatus dangerous.
Voltage Switch
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Intentional interruption is prohibited.
Whenever it is likely that the protection has been impaired, the apparatus shall be made
inoperative and be secured against any unintended operation.
NEVER touch or handle the power supply on MAPADA UV/V-1600/1800 due to the high
voltage.
The protection is likely to be impaired if, for example, the apparatus
Shows visible damage
Fails to perform the intended measurements
Has been subjected to prolonged storage under unfavorable conditions
Has been subjected to severe transport stresses
Working Principle:
The spectrophotometer consists of five parts: 1) Halogen or deuterium lamps
to supply the light; 2) A Mono-chromator to isolate the wavelength of interest
and eliminate the unwanted second order radiation; 3) A sample compartment
to accommodate the sample solution; 4) A detector to receive the transmitted
light and convert it to an electrical signal; and 5) A digital display to indicate
absorbance or transmittance. The block diagram (Fig 1-2) below illustrates
the relationship between these parts.
Block diagram for the Spectrophotometer
Light Mono- Sample Detector Display
Source chromator Compartment
Fig1-2
In your spectrophotometer, light from the lamp is focused on the entrance slit of the
monochromator where the collimating mirror directs the beam onto the grating. The
grating disperses the light beam to produce the spectrum, a portion of which is focused on
the exit slit of the monochromator by a collimating mirror. From here the beam is passed
to a sample compartment through one of the filters, which helps to eliminate unwanted
second order radiation from the diffraction grating. Upon leaving the sample
compartment, the beam is passed to the silicon photodiode detector and causes the
detector to produce an electrical signal that is displayed on the digital display.
Unpacking Instructions:
Carefully unpack the contents and check the materials against the following packing list to
100%T
0
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ensure that you have received everything in good condition.
Packing List
Description Quantity
Spectrophotometer ............................................................... 1
Mains Lead ........................................................................... 1
Cuvettes................................................................................ Set of 4, glass
.......................................................................... Set of 2, quartz
Manual .................................................................................. 1
Note: the Quartz cuvette does not come standard with the model of V1600 and v1800..
Specifications:
Installation:
1. After carefully unpacking the contents, check the materials with the packing list
(page 2) to ensure that you have received everything in good condition.
Model V-1600 V-1800 UV-1600 UV-1800
Wavelength Range 320-1100nm 190-1100nm
Spectral Bandwidth 4nm 2nm 4nm 2nm
Optical System Single Beam, Grating 1200lines/mm
Wavelength Accuracy ±0.5nm
Wavelength Repeatability 0.3nm
Wavelength Resolution ±0.1nm
Photometric Accuracy ±0.3%T
Photometric Repeatability ±0.2%T
Photometric Range -0.3-3A, 0-200%T
Stray Light 0.05%T@360nm 0.05%T@220nm,340nm
Stability ±0.002A/h @500nm
Display Graphic LCD (128X64 dots)
Keyboard 22 Membrane keypad
Photometric Mode T, A, E
Detector Si Photodiode
Sample Compartment Standard 10mm path length cuvette
Accommodates 100mm path length cuvette with optional holder
Light Source Tungsten lamp Tungsten & Deuterium
Output USB Port Parallel Port (printer)
Power Requirement AC 220V/50Hz or 110V/60Hz
Dimensions (W x D x H) 470 x 370 x 180mm
Weight 12kg 14kg
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2. Place the instrument (Fig.1-3) in a suitable location away from direct sunlight.
In order to have the best performance from your instrument, keep it as far as
possible from any strong magnetic or electrical fields or any electrical device that
may generate high-frequency fields. Set the unit up in an area that is free of
dust, corrosive gases and strong vibrations.
Fig.1-3
3. Remove any obstructions or materials that could hinder the flow of air under and
around the instrument.
4. Use the appropriate power cord and plug into a grounded outlet.
Fig.1-4
5. Turn on your MAPADA UV/V-1600/1800 model spectrophotometer. Allow it to
warm up for 20 minutes before taking any readings. We suggest you then do the
Calibrate System with the Search 656.1nm to set the wavelength to the
deuterium lamp emission line.
NOTE:
This symbol means Caution, Risk of Danger.
Control Panel
LCD
Compartment
Holder Changer Knob
Fan
Parallel Port USB Port Power Cord Inlet
Fuse
Power Switch
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Operation:
Prepare the spectrophotometer
Fig 1-2 is the control panel. User can perform all operations by pressing the keys and all
the results and operation information are displayed on the LCD.
Fig 2
Description of keys
【 START/STOP 】 Start/stop testing
【 RETURN 】 Exit to previous screen or cancel the operation;
【 ENTER 】 Confirm the inputted data or selected item;
【 CLEAR 】 Clear all characters when you are inputting or clear test results display
on the screen;
【 SET 】 Parameter set
【 GOTO λ
λλ
λ 】 Set wavelength;
【
ZERO 】 Blank
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【 PRINT 】 Print test results
【 0 】-【 9 】 Input number or menu select.
【 . 】 Input dot;
【 - 】 Input minus symbol;
【  】 , 【  】 Scroll items for selecting; Browse the items for selection;
Turn on spectrophotometer
Turn on spectrophotometer by pressing the Power Switch (IO). The instrument starts to
initiate and the steps are as below:
1.The instrument will position filter first, then initialize lamp position and AD
converter, then D2 / W lamps warm up, then Wavelength reset and get dark current, After
initializing printer, checking battery and setting up system, the screen display the main
menu. And the self-testing process display as fig 3.
2 .If the data in memory has been lost, the instrument will directly calibrate system
without any choice for you.
Note: DO NOT OPEN SAMPLE COMPARTMENT LID DURING SELF-TESTING.
Fig 3
√ Self-testing
●
●
●
●Filter √
√ Self-testing
○
○
○
○D2 Lamp √
●
●●
●Basic Mode
○
○○
○Quantitative
√ Self-testing
○
○○
○Dark Current √
√ Self-testing
○
○○
○System √
Main Menu
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Basic operation
Blank
Push the blank cuvette into the light path.
Press the key 【 ZERO 】 for blanking
Note:1 、
、、
、 If the reference solution is too thick, the energy will be low.
2
、 .Blanking is automatic after a wavelength change.
DO NOT OPEN SAMPLE COMPARTMENT LID DURING BLANKING.
Set Wavelength (Example: set wavelength in “Basic mode”)
Press 【 GOTO λ
λλ
λ 】 (Fig 4).
GOTO λ
λλ
λ
RETURN
Fig 4
Use numeric keypad to input wavelength (e.g. 500nm).
Press 【 ENTER 】 to change the wavelength, and then blank; After blanking,
the screen displays as the left one of Fig 5.
Note:1. If you don’t want to change the wavelength, you can press 【 RETURN 】
】】
】.
..
.
2. the wavelength range is 190-1100, other figure is invalid.
START
RETURN
Fig. 5
Measurement (Example: set wavelength in “Basic mode”)
Push the blank cuvette into the light path. Then press
【 ZERO 】
】】
】.
..
.
Basic Mode：
：：
：
WL=546.0nm
Please Input New
WL=
Basic Mode：
：：
：
0.000 Abs
546.0 nm
Basic Mode：
：：
：
0.000 Abs
500.0 nm
500.0nm 0.000Abs
No. WL Abs
1500.0 0 .000
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Press 【START/STOP】in the left one of Fig. 5 to go into testing
interface.(The right one of Fig 5)
Press 【START/STOP】again in the right one of Fig. 5, the testing
result displays on the screen, repeat this step, the results will
display on the screen one by one.
Print test report (For example: Print the report in “Basic mode”, the right one of Fig. 5)
Press
【 PRINT 】 in the right one of Fig.5 to print the report (Fig. 6)
Fig 6
Note: After printing, all the data displayed on the screen will disappear.
Before measurement
Make a blank reference solution by filling a clean cuvette (or test tube) half full with
distilled or de-ionized water or other specified solvent. Wipe the cuvette with tissue
to remove the fingerprints and droplets of liquid. Fit the blank cuvette into the 4-cell
linear changer and place the cuvette in the slot nearest you. For the MAPADA
UV/V-1600/1800, push the changer so that the cuvette is in the light path (Push the
rod in). Close the lid.
Analyze Sample
We provide three test methods in uv-16/18 series, they are basic mode test, quantitative
and kinetics.
1. Basic Mode
Push the blank cuvette into the light path. In main menu (the last one of Fig. 3), move the
cursor on “Basic mode”, then press 【 ENTER 】 to go into basic mode test. After
automatically blanking, it will display as the left one of Fig. 4 and wait for the operator.
【 RETURN 】 to exit.
Select Test Mode
There are three test modes (T%, Abs, Conc. / factor) for you to select by pressing
Basic Mode
NO. Abs. T%
1 0.000 100.0%
2 0.000 100.0%
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【 SET 】 to make choice.
【 SET 】
【 RETURN
RETURNRETURN
RETURN 】
Fig.7
Use 【  】 and 【  】 to move the cursor on ●
●●
●Abs. , then press 【 ENTER 】, If the icon
“√
√√
√”appears at the end of ●
●●
●Abs. , you have selected successfully. Press
【 RETURN
RETURNRETURN
RETURN 】 to exit to the left of Fig.4, blank at the same time. (Fig. 7)
The operation is the same as Abs test mode to select T% mode and Energy mode.
Begin to Test
Push the sample into light path, press 【 START
STARTSTART
START 】 to go into basic mode test
interface.(Fig. 8)
START
RETURN
Fig. 8
Press 【 START
STARTSTART
START 】 again, the test result will display on the screen. The operation is the
same if you change your samples.
Note:
① 5 groups of test data can display per screen, total 200 groups of data can be saved.
② In the right one of Fig. 8, you can change your test wavelength, blank, change test
mode and print by press
【 GOTO
GOTO GOTO
GOTO λ
λλ
λ 】, 【 ZERO
ZEROZERO
ZERO 】 , 【 SET
SETSET
SET 】 and 【 PRINT
PRINTPRINT
PRINT 】 respectively.
You can also clear the test data by press
【 CLEAR
CLEARCLEAR
CLEAR 】.
√
√√
√Basic Mode:
●
●●
●Abs. √
√√
√
○
○○
○
T%
Basic Mode：
：：
：
0.000 Abs
546.0 nm
Basic Mode：
：：
：
0.000 Abs
500.0 nm
500.0nm 0.000Abs
No. WL Abs
1500.0 0 .000
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2. Quantitative Mode
It includes two test methods, one is Standard curve, the other is Coefficient method. In the
former method, you can establish a standard curve using standard samples known
concentration. If you have known a curve equation, you can select the latter method.
Enter Quantitative Mode
Move the cursor on the Quantitative Mode, Press 【 ENTER 】 to go into the
method choosing interface.(Fig. 9)
ENTER
RETURN
Fig.9
2-1. Standard Curve Method
It’s a method to establish a standard curve by measuring a group of standard
samples.
Move the cursor on the Standard Curve, after 【ENTER】being expressed, it
goes into the pre-testing interface.（
（（
（Fig.10）
））
）
ENTER
RETURN
Fig. 10
Set Wavelength
Press 【GOTO λ】in the pre-testing interface, input the testing
wavelength by pressing the numeric keypad. (Fig.11). Reference Page
√
√√
√Quantitative
●
●●
●Standard Curve
○
○○
○
Coefficient
○
○○
○Basic Mode
●
●●
●Quantitative
○
○○
○
Kinetics 08:00
√
√√
√Quantitative：
：：
：
●
●●
●Standard Curve
○
○○
○
Coefficient
Standard Curve
0.000 Abs
0.000 ml/l
546.0 nm
Current WL.
Current Abs.
Concentration
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7, you’ll get a detailed instruction.
GOTO λ
λλ
λ
RETURN
Fig. 11
Blank
Pull the blank solution in the light path, then press 【 ZERO 】 .
Set parameters
Press 【SET】in the pre-testing interface. It goes into parameters setting
interface. (Fig.12). You should set Unit, the Number of standard samples,
Concentration of standard samples before testing.
SET
RETURN
Fig. 12
Choose unit of standard samples
Move the cursor on “Unit” by pressing 【  】 and 【  】 , followed by pressing
【 ENTER 】 , 8 units are under your selection. (Fig 13)
ENTER
RETURN
Fig.13
Move the cursor on the unit you need, then press 【 ENTER 】 . You can also
√
√√
√Standard Curve
●
●●
●Unit
√
√√
√Standard Curve
○
○○
○Display Curve
Standard Curve
0.000 Abs
0.000 ml/l
546.0 nm
Standard Curve
0.000 Abs
0.000 ml/l
546.0 nm
Standard Curve
WL＝
＝＝
＝546.0nm
Please Input New：
：：
：
√
√√
√Standard Curve
●
●●
●Unit
○
○○
○
Number
1. % 5. ml/l
2. ug/l 6.mg/ml
3. mg/l 7.ug/ml
4. g/l 8.none
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press the number ahead of the unit directly by the numeric keypad.
If you don’t want to select any item, press 【 RETURN
RETURNRETURN
RETURN 】 to go back to last interface.
Set Quantity of Standard Samples
Move the cursor on “Number” by pressing 【  】 and 【  】 , followed by
pressing
【 ENTER 】 . (Fig. 14)
ENTER
RETURN
Fig. 14
Input the number of your standard samples by numeric keypad. After
pressing 【 ENTER 】 ,it returns to the setting interface.
Note：the number range is 1-9，other number is invalid.
Set Concentration of Standards
Before this step, you must blank first. Pull the blank cuvette in the light
path in the pre-testing interface, press 【 ZERO 】 .
Move the cursor in “Concentration”, following press 【 ENTER 】 .(Fig. 15)
ENTER
RETURN
Fig.15
Pull sample 1 in the light path, input the concentration of sample 1 by
numeric keypads, then press 【ENTER】. It goes into the next setting
interface of sample 2, the operation is the same as sample 1. Repeat the
operation one by one till the last sample finished, and it goes back to the
setting interface.
√
√√
√Standard Curve
○
○○
○Unit
●
●●
●
Number
Standard Curve
N=2
Please Input New：
：：
：
N=
Pull Sample 1
C=1.500
Please Input New
C=
√
√√
√Standard Curve
○
○○
○Unit
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Note: ①The Abs. or T% of the samples will not display on the screen,
they were saved in the RAM.
The range or concentration is 0-9999，other number is invalid.
③The sequence of concentration is from low to high.
Display Standard Curve
Move the cursor on “Display Curve” by pressing【】and 【】, followed
by pressing 【ENTER】, the standard curve you just established will display
on the screen. The equation displays on the bottom of the screen. (Fig. 16),
and it will also be saved in the system.
1
ENTER
2
RETURN
3
Fig. 16
1、Standard Curve
2、Relative Coefficient.
3、Curve Equation
C=1.001*A+0.2,
C: Concentration
A: Value of Abs.
The slop value of the curve is 1.001.
The intercept of the curve is 0.2.
If the system gives a fault hint, check the standard sample and your inputting,
press any key to return and repeat your performance. (Fig. 17)
ENTER
√
√√
√Standard Curve
○
○○
○Concentration
●
●●
●
Display Curve
0.500A
0
r=0.999 0.8C
C=1.001*A+0.2
√
√√
√Standard Curve
○
○○
○Concentration
●
●●
●
Display Curve
Establish Wrong！
！！
！
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Any key
Fig. 17
Select Curve
Move the cursor on “ Select Curve” by pressing 【  】 and 【  】 ,then press
【ENTER】to confirm. (Fig. 18)
ENTER
RETURN
Fig. 18
The newly established curve equation will be displayed at the end of the
group. Total 200 curve equations can be saved.
Move the cursor on the equation you need by pressing 【  】 and 【  】 ,then
it returns to setting interface after 【ENTER】being pressed. Press 【RETURN】
to go back to pre-testing interface.
Note: If you want to delete a equation, move the cursor on it, just
press 【
【【
【CLEAR】
】】
】.
Test and Print
When you have selected the equation, press 【START/STOP】to go into testing
interface in pre-testing interface.(Fig. 19)
START
RETURN
Fig. 19
After blanking, pull the unknown sample cuvette in the light path, then press
Standard Curve：
：：
：
0.000 Abs
0.000 ml/l
546.0 nm
546.0nm -0.000Abs
No. WL Abs Conc
1546.0 0.001 1.0 g/l
√
√√
√Standard Curve
○
○○
○Concentration
○
○○
○
Display Curve
No. Para
1. C=0.860*A+0.000 *
2. C=0.732*A+0.125
3. C=0.005*A+0.002
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【ENTER】, the testing result will be displayed on the screen. Operation is the
same if you want to test other samples.
Press 【PRINT】to print testing results. （Fig. 20）
Fig. 20
2-2. Coefficient Method
If you have known the standard curve equation, you can use this method to test.
Move the cursor on the Coefficient by pressing 【  】 and 【  】 , after 【ENTER】
being expressed, it goes into the formula selecting interface.（
（（
（Fig.21）
））
）
ENTER
RETURN
Fig. 21
Choose the formula you need by pressing “1” or “2” of the numeric keypad,
you’ll enter the coefficient method pre-testing interface.(Fig.22)
You can also select by pressing 【  】 and 【  】 followed by【ENTER】.
1↙
2↙
Coefficient
1. A=KC+B
2. C=KA+B
√
√√
√Quantitative
○
○
○
○Standard Curve
●
●●
●
Coefficient
Coefficient
1. A=KC+B
2. C=KA+B
Coefficient: A=KC+B
0.001 Abs
1.200 mg/l
546.0 nm
Standard Curve
NO. Abs. T% Conc.
1 0.000 100.0% 0.0g/l
2 0.000 100.0% 0.0g/l
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RETURN
+B
RETURN
Fig. 22
Note: The two formulas can be converted each other, it’s up to your habit to
choose anyone of them, but the operation is the same. C=KA+B is an example.
Set Wavelength
Press【GOTO λ】in the pre-testing interface, input the testing wavelength by
pressing the numeric keypad. (Fig.23). Reference Page 7, you’ll get a detailed
instruction.
GOTO λ
λλ
λ
RETURN
Fig. 23
Set Parameters
Press 【SET】in the coefficient method pre-testing interface, 3 parameters
should be set before testing.(Fig. 24)
SET
RETURN
Fig. 24
Set Coefficient K
Move the cursor on “Coefficient K” by pressing 【 ∧
∧∧
∧ 】 and 【 ∨
∨∨
∨ 】 , followed by
【 ENTER 】 .(Fig. 25)
ENTER
√
√√
√Quantitative：
：：
：
○
○○
○Standard Curve
●
●●
●
Coefficient
Coefficient: C=KA+B
0.001 Abs
1.000 mg/l
546.0 nm
Coefficient: A=KC+B
0.001 Abs
1.200 mg/l
546.0 nm
√
√√
√Coefficient
●
●●
●Coefficient K
○
○○
○
Coefficient B
√
√√
√Coefficient
●
●●
●Coefficient K
○
○○
○
Coefficient B
Coefficient
K=4.000
Please Input New
K=
Coefficient: A=KC+B
0.001 Abs
1.200 mg/l
546.0 nm
Coefficient：
：：
：
WL=546.0nm
Please Input New：
：：
：
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RETURN
Fig. 25
Input the new value of K by pressing the numeric keypad followed by
【 ENTER 】 ,it will return the setting interface.
Note: the range of K is 0-9999, other value is invalid.
Set Coefficient B
Move the cursor on “Coefficient B” by pressing 【 ∧
∧∧
∧ 】 and 【 ∨
∨∨
∨ 】 , followed by
【 ENTER 】 .(Fig. 26)
ENTER
RETURN
Fig. 26
Input the new value of K by pressing the numeric keypad followed by
【 ENTER 】 ,it will return the setting interface.
Note: the range of B is -9999-9999, other value is invalid.
Set Concentration Unit
Move the cursor on “Conc. Unit” by pressing 【 ∧
∧∧
∧ 】 and 【 ∨
∨∨
∨ 】 , followed by
【 ENTER 】 .(Fig. 27)
ENTER
RETURN
Fig. 27
Move the cursor on the unit you need, then press 【 ENTER 】 . You can also
press the number ahead of the unit directly by the numeric keypad.
√
√√
√Coefficient
○
○○
○Coefficient K
●
●●
●
Coefficient B
Coefficient
B=4.000
Please Input New
B=
√
√√
√Coefficient
○
○○
○Coefficient K
○
○○
○
Coefficient B
1.% 5. ml/l
2. ug/l 6.mg/ml
3. mg/l 7.ug/ml
4. g/l 8.none
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1600/1800 Manual
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Press 【 RETURN 】 to go back to pre-testing interface.
Blank
Pull the blank solution cuvette into the light path, then press 【 ZERO 】 .
Test and Print
Pull the unknown concentration sample in the light path, then press
【 START 】 , now it enters the testing interface. Press 【 START 】 again, you’ll get
the concentration of the sample.(Fig. 28)
START
RETURN
Fig. 28
The operation is the same if you have more samples to test.
Press 【PRINT】to print the test results. All the data will be deleted after
printing.
You can also delete the test results by pressing 【CLEAR】.
3. Kinetics Mode
Move the cursor on “Kinetics” by pressing【∧
∧∧
∧】and 【∨
∨∨
∨】in the main menu,
then press 【ENTER】to go into kinetics pre-testing interface. （Fig.29）
ENTER
RETURN
Coefficient: A=KC+B
0.001 Abs
1.200 mg/l
546.0 nm
546.0nm -0.000Abs
No. WL Abs Conc
1546.0 0.001 1.0 g/l
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○Basic Mode
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○Quantitative
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Kinetics 08:00
Kinetics
100.0 T%
546.0 nm
DOMINIQUE DUTSCHER SAS