Microtek Bio-6000 User manual

Getting to KnowYour Bio-6000
The Bio-6000 is a professional gel scanner specially designed and optimized for image
capture of biological samples from gels or transparent film. It features anA3-sized
scan bed, 48-bit color depth, 3200 x 6400-dpi optical resolution, and one Smart-Touch
button on the front panel for an easy access to the scan function. With a Dmin of
0.05DandaDmaxof3.77D, theBio-6000candeliverawidedynamicrangeto
enhance the image quality in both bright and dark areas of the image. Additionally,
the Bio-6000 also lets you scan reflectives and prints up to 11.6" x 16.9" in size,
convenient for any biological laboratory use.
Copyright © 2014 Microtek International, Inc. http://www.microtek.com
I49-004826 E, January 2014
Contents
Getting to KnowYour Bio-6000 ....................................................................... 1
Features of the Bio-6000 .................................................................................. 2
Taking a Closer Look ........................................................................................ 3
Initial Setup ...................................................................................................... 4
Step 1: Unpacking Package Contents ................................................... 4
Step 2: Installing the Software .............................................................. 5
Step 3: Unlocking the Scanner .............................................................. 6
Step 4: Installing theTMA ...................................................................... 7
Step 5: Connecting the Scanner ............................................................ 8
Using the U-Shaped Ruler (Calibration Ruler) ............................................... 9
Positioning Electrophoresis Gels/Film or Transparent Film ....................... 10
Positioning Reflective Materials ................................................................... 10
The Black Plate ................................................................................... 11
Your First Scan ............................................................................................... 12
Scanning Scenarios ....................................................................................... 14
Scanning Electrophoresis Gels or Film ................................................ 15
Scanning Reflective Materials ............................................................... 17
Specifications / System Requirements ........................................................ 19
FCC Compliance Statement .......................................................................... 20
Bio-6000 features, scenarios, and information
User Guide

2 Bio-6000 User Guide
The Bio-6000 comes with several important features, including the following:
• LargeA3 scan bed — TheA3-sized scan bed of the Bio-6000 lets you easily scan
biological samples from a gel or film up to 11.6" x 16" and oversized reflective
originals up to 11.6" x 16.9", such as contact sheets, large pieces of art, and
mechanical blueprints.
• 3200 x 6400-dpi resolution: The exceptionally high resolution of the Bio-6000 lets
you scan even postage-size images and enlarge them with amazing clarity, with
little loss of detail. The scanner’s 3.77 maximum optical density allows it to capture
a wide range of tones approximating real-life color and hues.
• Smart-Touch button: ASmart-Touch button (Scan) locating at the front panel of
the scanner provides you a quick and easy way to capture images that can be
automatically saved as files or sent to another application for further processing
later.
• Energy-saving LED light source: Adopting LEDs as the light source, when the
scanner is detected by the system, there are no requirements for any warm-up time
before carrying out the scan, which boots your productivity and reduces energy
costs amazingly. With its stable performance, the image quality will remain
consistent even after used for a certain period of time.
• Microtek ScanWizard™ Bio scanning software: This is a scanning software for
the use of the electrophoresis gel/film scanning. Friendly and easy-to-use
interface with powerful functions and controls offer you a quick access and
management for all your scanning tasks.
• Microtek's MiBio software: This is a management software developed exclusively
for Bio-tech analysis and research. It features several easy-to-use but very
practical functions, supporting users with powerful tools in a clear and simple
control panel.
Features of the Bio-6000

Bio-6000 User Guide 3
Taking a Closer Look
TMA
Scanner Glass surface
(Scan bed)
Vertical ruler
READY indicator (Green)
POWER indicator (Orange)
Power connector
Accessory port (or with TMA's
connector securely connected)
Hi-Speed USB port (1)
Power switch
Smart-Touch button (Scan)

4 Bio-6000 User Guide
Initial Setup
Step 1: Unpacking Package Contents
After unpacking your scanner package, please check for the major components listed
below.
1. Bio-6000 (lower base)
2. USB Cable
3. Power Cord
4. Software CDs
5. TMA (Transparent Media Adapter)
6. Black Plate - for scanning reflective media
7. U-shaped Ruler (Calibration Ruler) - for calibrating your scanner
1
2
4
3
5
6
7

Bio-6000 User Guide 5
Step 2: Installing the Software
Important: Do not remove the yellow sticker from your scanner until you are
told to do so. You must install software before connecting your scanner.
Always close any open programs and turn offAnti-virus utilities before installing
software.
1. PlacetheAdobeCD-ROMintothe CD-
ROM drive, and install the software.
Skip this step if you have a newer
version of Adobe Photoshop Elements
already installed on your computer.
2. PlacetheMicrotek softwareCD-ROM
into the CD-ROM drive, then follow the
on-screen instructions to install the
driver and software.
NOTE: If the Microtek Software Installer screen does not come up automatically,
double-click the following in succession: “My Computer”; the CD-ROM icon;
then cdsetup.exe to start the installer program.
3. Restart your computer at the end of all software installation.
Drivers & Software Upgrades
After you finish the installation of software, if you found that the installed drivers and software cannot
run your product or your computer system properly later, please go to the Microtek Download Service
site at ww7.microtek.com.tw/service.php to download and install any updates you may require.
For additional information about Microtek products, please visit our website at www.microtek.com.

6 Bio-6000 User Guide
Step 3: Unlocking the Scanner
Before you operate the scanner, you will need to unlock the scanner. To unlock the
scanner, follow the steps below:
Unlocked
Locking the Scanner
To lock the scanner (for shipping and other purposes), follow the steps below:
1. Turn off the scanner.
2. Turn the scanner back on.
When the indicators on the front of
your scanner stop blinking, use a
screwdriver, and then push and turn
the locking screw clockwise to the
locked position.
When the screw has been tightened,
this indicates that your scanner is
locked.
3. Turn off your scanner.
Locked
1. Removethe yellow “Unlock” sticker from
your scanner.
2. With the scanner power off, turn the
scanner on its side and locate the
locking screw at the bottom of the
scanner.
3. Using a screwdriver, push and turn the
locking screw counterclockwise to the
unlock position.
When successfully unlocked, the screw
will push out a little, protruding slightly
from the bottom of the scanner.

Bio-6000 User Guide 7
Locking theTMA
To lock the TMA(for shipping and other purposes), follow the steps below:
Step 4: Installing the TMA
1. Attach the TMA by sliding the posts at the rear of the TMA into the holes.
Unlocked
2. Plug the connector of the TMA into the
scanner’s 15-pin accessory port.
1. Make sure the TMA is connected to the
scanner, then turn off the scanner if
your scanner is on.
2. Turn the scanner back on. The TMA's
carriage will move to the standby
position in a few moments.
3. When the indicators on the front of
your TMA and scanner stop blinking,
turn the locking knob clockwise to the
lock position “ ”.
4. Turn off your scanner and disconnect the TMA connector from the scanner. The
TMA is ready for transport.
Locked
3. Unlock the TMA.
a) Remove the yellow “Unlock” sticker from the TMA.
a) Look for the locking knob at the base of the TMA.
b) Turn the locking knob counterclockwise to the unlock position “ ”.

8 Bio-6000 User Guide
Step 5: Connecting the Scanner
To the power adapter
1. Connect the power cord to the back of the scanner.
2. Plug the power cord into a power source.
To the Hi-Speed USB cable
3. Connect one end of the cable to your computer.
4. Connect the other end of the cable to the scanner.
5. Press the power switch at the back of your scanner, and wait for the indicator light
on the front panel to stop blinking and stay on steady.
The system will detect your scanner automatically.

Bio-6000 User Guide 9
Using the U-shaped Ruler (Calibration Ruler)
The U-shaped Ruler (Calibration Ruler) is designed for the use of the scanner
calibration. Please take note that before putting the U-shaped Ruler on the scanner
glass surface, make sure the scanner is turned off.
To use the U-shaped Ruler:
1. Peel the protective film from the backside of the rulerbefore using it to calibrate
the scanner.
Silicone Rings U-shaped Ruler
2. Then, put the ruler facing up on the scanner glass surface and hook the ruler onto
the raised Silicone Rings located on the both sides of the scanner surface, making
sure that they have been oriented correctly.
3. Finally, turn on the scanner power.

10 Bio-6000 User Guide
Positioning Reflective Materials
To scan reflective materials (such as photo, prints, dissertations, papers, etc.), you
have to scan the reflective original together with the U-shaped Ruler (Calibration
Ruler)andBlack Plate. Before positioning the reflective, make sure that the U-
shapedRuleris on the scannerglass surface properly.
1. Place the reflective original to be
scanned face down on the scanner
glass surface, with the top end of it
toward the front of the scanner.
2. Peel the protective film from the Black
Plate*.
3. Put the Black Plate on the top of the
reflective to cover it, with the side of
the Black Plate that reads “This side
up” facing up.
Positioning Electrophoresis Gels/Film or
Transparent Film
To scan the electrophoresis gels/film (such as 1D/2D gels, SDS-PAGE, western/
northern/southern blots, or membranes) and transparent film (such as slide, positive
film, or reversal film), you have to scan gels or film together with the U-shaped Ruler
(CalibrationRuler).
U-shaped Ruler
• To scanwith U-shaped Ruler, referto
the “Using the U-shaped Ruler”
section in this guide to place the U-
shaped Ruler first, then place and
center the gel or film on the scanner
glass surface. Make sure to keep the
area of the calibration strip clear.
U-shaped Ruler
Black Plate
Gel or film
Reflective
Calibration
strip

Bio-6000 User Guide 11
*The Black Plate
Light from the TMA and other ambient light during scanning may result in overexposed images. The
Black Plate is designed to work with reflective originals.To ensure correct exposure and optimal
image quality during scanning, the Black Mat should be used to shield unwanted light out.
Take note of the following before using the Black Plate.
1. The Black Plate may become dirty over time and with
prolonged use. To clean the plate, use a white cotton cloth
dipped in some water, and gently wipe the plate surface. This
ensures that the Black Plate is in optimal condition at all times.
2. A protective film covers the Black Plate during shipping and
prevents it from being scratched. When peeling the film from
the plate, be careful not to tear or bend the Black Plate.

12 Bio-6000 User Guide
Your First Scan
This section is to guide you to perform your first scan by using the ScanWizard Bio.
You should familiarize yourself with the basic scanning technique before you
continue further scanning scenarios described in next pages.
1. Launch ScanWizard Bio either as a stand-alone by clicking on the program icon,
or by using the File-Import or File-Acquire command from your image-editing
program.
2. Place the gel or film to be scanned
facing down on the scanner glass
surface, with the top end of it toward
the front of the scanner. Remember to
keep the calibration strip clear.
3. Go to the Preview window of
ScanWizard Bio, and choose
Transmissivefrom the Scan Material
menu.
U-shaped Ruler
4. Click the Overviewbutton to perform a preliminary scan of the image, which will
appear in the Preview window.
5. Select the Scan Frame tool from the Toolbar in the Preview window, and choose
the area to be scanned by dragging a rectangle around it. You will see a flashing
frame (marquee) around the selected area.
6. Click the Prescan button to display a detailed image of area selected by the
Scan Frame tool.Athumbnail of the image appears as well in the Scan Job
Queue window.
7. Specify your scanning requirements in the Settings window.
a) Select a desired image type.
b) Select a desired resolution.
c) Adjust the scan frame settings if necessary.
8. Adjust image quality if necessary, using theAdvanced Image Correction (AIC)
tools.
9. Click the Scan to (or “Scan”) button in the Preview window or on the scanner
unit to start scanning.
• If ScanWizard Bio was launched from an application program, the image is
then delivered to your application, where the image can be saved, printed, or
edited.

Bio-6000 User Guide 13
• If ScanWizard Bio was launched in stand-alone mode, you will be prompted to
specify the file attributes for the scanned image after the Scan or Scan to
button is pressed, such as entering the file name, specifying a folder name of
your own, etc. When you have completed the settings, press the Done/Save
button, and the scanner will automatically scan and save your image based on
your settings.
Preview window
Settings window
Scan Job window

14 Bio-6000 User Guide
Scanning Scenarios
The following pages provide various scenarios for scanning with the Bio-6000,
including the following:
• Scanning electrophoresis gels or film: This scenario details the steps for scanning
electrophoresis gels or film.
• Scanning reflective originals: This scenario details the steps for scanning
reflective materials, such as photos or prints.

Bio-6000 User Guide 15
Scanning Electrophoresis Gels or Film
Gel or film
1. Place the gel or film to be scanned on the
scanner glass surface, as detailed in the
“Positioning Electrophoresis Gels/Film or
Transparent Film” section of this guide.
2. Launch ScanWizard Bio either as a
stand-alone by clicking on the program
icon, or by using the File-Import or File-
Acquirecommandfromyour image-
editing program.
3. Go to the Preview window and choose Transmissivefrom the ScanMaterial
menu.
4. Click the Overview button to perform a preliminary scan of your original.
5. Select the Scan Frame tool from the Toolbar in the Preview window, and choose
the area to be scanned by dragging a rectangle around it. You will see a flashing
frame (marquee) around the selected area.
6. Click the Prescan button to display a detailed image area selected via the
Scan Frame tool.Athumbnail of the image appears as well in the Scan Job
Queue window.
7. Specify your scanning requirements in the Settings window.
a) Select a desired image type.
b) Select a desired resolution.
c) Adjust the scan frame settings if necessary.
8. Adjust image quality if necessary, using theAdvanced Image Correction (AIC)
tools.
9. Click the Scan to (or “Scan”) button in the Preview window or on the scanner unit
to start scanning.
• If ScanWizard Bio was launched from an application program, the image is then
delivered to your application, where the image can be saved, printed, or edited.
• If ScanWizard Bio was launched in stand-alone mode, you will be prompted to
specify the file attributes for the scanned image after the Scan or Scan to
button is pressed, such as entering the file name, specifying a folder name of
your own, etc. When you have completed the settings, press the Done/Save
button, and the scanner will automatically scan and save your image based on
your settings.

16 Bio-6000 User Guide
Preview window
Settings window
Scan Job window

Bio-6000 User Guide 17
Scanning Reflective Materials
Reflective
Black Plate
1. Place the reflective original to be
scanned on the scanner glass surface,
as detailed in the “Positioning
Reflective Materials” section of this
guide.
2. Launch ScanWizard Bio either as a
stand-alone by clicking on the program
icon, or by using the File-Import or File-
Acquirecommandfromyour image-
editing program.
3. Go to the Preview window of ScanWizard Bio, and choose Reflective from the
ScanMaterial menu.
4. Click the Overviewbutton to perform a preliminary scan of the image, which will
appear in the Preview window.
5. Select the Scan Frame tool from the Toolbar in the Preview window, and choose
the area to be scanned by dragging a rectangle around it. You will see a flashing
frame (marquee) around the selected area.
6. Click the Prescan button to display a detailed image area selected via the
Scan Frame tool.Athumbnail of the image appears as well in the Scan Job
Queue window.
7. Specify your scanning requirements in the Settings window.
a) Select a desired image type.
b) Select a desired resolution.
c) Adjust the scan frame settings if necessary.
8. Adjust image quality if necessary, using theAdvanced Image Correction (AIC) tools.
9. Click the Scan to (or “Scan”) button in the Preview window or on the scanner unit
to start scanning.
• If ScanWizard Bio was launched from an application program, the image is then
delivered to your application, where the image can be saved, printed, or edited.
• If ScanWizard Bio was launched in stand-alone mode, you will be prompted to
specify the file attributes for the scanned image after the Scan or Scan to
button is pressed, such as entering the file name, specifying a folder name of
your own, etc. When you have completed the settings, press the Done/Save
button, and the scanner will automatically scan and save your image based on
your settings.

18 Bio-6000 User Guide
Preview window
Settings window
Scan Job window

Bio-6000 User Guide 19
System Requirements
GeneralRequirements
• CD-ROMdrive(forinstalling software)
• Color display with 24-bit color output capability
• 512MB RAM or more
PCandcompatibles
• PentiumIV PC or higher withHi-SpeedUSB (USB 2.0) port
• Microsoft Windows 7 and 8; compatible with Windows XP andVista
Specifications
Scanning Modes Color and grayscale in a single scanning pass
True 48-bit color (approx. 281 billion colors)
16-bit grayscale (approx. 65,536 shades of gray)
Light Source LED
Scanning Area Reflective:11.6" x16.9" (294.6mmx429.3mm)
Transparent:11.6" x16"(294.6 mmx 406.4mm)
Resolution Optical:3200 (H) dpix 6400(V)dpi
Dynamic Range Transparency: 0.05D ~ 3.77D,3.77 Dmax
Interface Hi-SpeedUSB(USB 2.0)
Dimensions(L x W x H) 24.7"x14.8"x7.3" (627.4mmx375.9mmx185.4mm)
Weight 35.4 lbs (16.1 kg)
Voltage AC100V-240V,47-63Hz,1.5Amax.
Power Consumption 54.9Wmax.
Environment Operating Temperature: 50° F to 104° F (10° C to 40° C)
RelativeHumidity:20%to85%
Important
Specifications, software bundles, and accessories are subject to change without
notice. Not responsible for typographic errors.

20 Bio-6000 User Guide
FCC Compliance Statement
This equipment (Model: MRS-6400A3PL) has been tested and found to comply with
the limits for a Class B digital device, pursuant to Part 15 of the FCC rules. These
limits are designed to provide reasonable protection against harmful interference in a
residential installation. This equipment generates, uses and can radiate radio
frequency energy and, if not installed and used in accordance with the instructions,
may cause harmful interference to radio communications. However, there is no
guarantee that interference will not occur in a particular installation. If this equipment
does cause harmful interference to radio or television reception, which can be
determined by turning the equipment off and on, the user is encouraged to try to
correct the interference by one or more of the following measures:
• Reorient or relocate the receiving antenna.
• Increase the separation between the equipment and receiver.
• Connect the equipment into an outlet on a circuit different from that to which the
receiver is connected.
• Consult the dealer or an experienced radio/TV technician for help.
NOTE: A shielded Hi-Speed USB interface cable with ferrite core installed on the
scanner connector and must be used with this equipment.
Caution: Changes or modifications not expressly approved by the manufacturer
responsible for compliance could void the user's authority to operate the equipment.
This device complies with Part 15 of the FCC Rules. Operation is subject to the
following two conditions: (1) This device may not cause harmful interference, and (2)
this device must accept any interference received, including interference that may
cause undesired operation.