Microtek Bio-1000F User manual

Gel Scanner
User's Manual
www.microtek.com

2 Bio-1000F User's Manual
Preface
Copyright
© 2016, Microtek International, Inc., all rights reserved. This document may not
be reproduced or copied in any way, stored electronically, or translated into any
language, without the permission of Microtek International, Inc.
Trademarks
Microtek, ScanMaker, ArtixScan, ScanWizard and ColoRescue are trademarks
or registered trademarks of Microtek International, Inc. All other trademarks or
registered trademarks are the property of their respective holders. Specications,
software and hardware bundles are subject to change without notice. Not
responsible for typographical errors.
Disclaimer
The contents of this manual have been checked carefully for accuracy, and every
effort has been made to ensure that there are no errors. However, Microtek
makes no guarantee as to the accuracy of the contents of this document and
reserves the right to make changes to the contents without prior warning.
I49-005119 A1
October 2016
Microtek International, Inc.
No. 6, Industry East Road 3, Science Based Industrial Park, Hsinchu, 30075, Taiwan
Tel: [phone], Fax: [phone], http://www.microtek.com

Bio-1000F User's Manual 3
FCC Compliance Statement
This equipment (Model: MFS-300L) has been tested and found to comply with
the limits for a Class B digital device, pursuant to Part 15 of the FCC rules.
These limits are designed to provide reasonable protection against harmful
interference in a residential installation. This equipment generates, uses and
can radiate radio frequency energy and, if not installed and used in accordance
with the instructions, may cause harmful interference to radio communications.
However, there is no guarantee that interference will not occur in a particular
installation. If this equipment does cause harmful interference to radio or
television reception, which can be determined by turning the equipment off and
on, the user is encouraged to try to correct the interference by one or more of
the following measures:
• Reorient or relocate the receiving antenna.
• Increase the separation between the equipment and receiver.
• Connect the equipment into an outlet on a circuit different from that to
which the receiver is connected.
• Consult the dealer or an experienced radio/TV technician for help.
NOTE: A shielded Hi-Speed USB interface cable with ferrite core installed on the
gel scanner connector end must be used with this equipment.
Caution: Changes or modications not expressly approved by the manufacturer
responsible for compliance could void the user's authority to operate the
equipment.
This device complies with Part 15 of the FCC Rules. Operation is subject to the
following two conditions: (1) This device may not cause harmful interference,
and (2) this device must accept any interference received, including interference
that may cause undesired operation.

4 Bio-1000F User's Manual
Safety Precautions
Before using your scanner, read the following safety guides carefully, which
detail the proper operation of the scanner and its accessories to prevent injuries
or damage to users or equipment.
Meanings of Symbol Signs
This indicates hazardous situation which, if not paid
attention, could result injury or damage to users or
equipment.
This indicates hazardous situation which, if not paid
attention, could result serious injury or death to users.
Power Supply:
• Use a power source only with a power rating specied on the marking label.
• Ensure to turn off the power of each device before connecting or
disconnecting the cables.
• Insert the plug completely into the outlet, as a loose connection may cause
arcing and result in re.
• Ensure to hold the plug or connector to disconnect the cable; otherwise,
if you pull the cable only, it may damage the core wire and result in re or
electric shock.
• Place and route the power supply cord such that it is not likely to be walked
on or pinched by items placed upon or against them, paying particular
attention to the cord near the power plugs, convenience receptacles, and at
the point where it exits from the outlet.
• When the scanner is left unattended and unused for long periods of time,
unplug it from the wall outlet.
Moving and Storing the Gel scanner:
• Always ensure that the scanner is stored properly before shipping or moving
it. Quick stops, excessive force, and uneven surfaces may cause the
product to overturn when moving.
• Do not hit or drop the scanner. The scanner may be damaged if it receives
a strong jolt, which may result in re or electric shock if the scanner is used
without being repaired.
• Do not place the scanner on any slippery, slanted, or unstable surface. The
product may slide or fall, causing serious injury to people as well as serious
damage to the product.
CAUTION
WARNING

Bio-1000F User's Manual 5
• Do not use the scanner near water. Never spill liquid of any kinds on the
product, or it may result in electric shock or other hazards.
• This scanner should be situated away from heat sources such as radiators,
heat registers, stoves, or other products (including ampliers) that produce
heat.
• Do not store the scanner in which it may be exposed to direct sunlight.
Using the Scanner
• Before using the scanner, make sure the area of the glass surface is clear
from obstacles.
Maintenance and Service:
• When the scanner is going to be cleaned, ensure to turn off the power of
each device and unplug the power cable from the AC outlet.
• When you need a repair service, unplug the scanner from the power outlet
and consult qualied service personnel.
• When replacement parts are required, use replacement parts that are
specied by the manufacturer or have the same characteristics as the
original parts.
• For safety reasons, ensure to inspect the device before using it. In addition,
carrying out a regular inspection at least once a year.
Blue-LED Light
• The blue-LED of the scanner is classied as Risk Group 1 (RG1), containing
very low risk. While the product is under a normal operation, the light
emitted from the blue-LED would not endanger human eyes
• In order to protect your eyes, do not stare directly at the light of the blue-
LED in any circumstances without using an appropriate shielding or lter
plate to unshield blue light.
• When using the blue-LED to perform gel excising or observation, ensure
that you use the lter plate came with your scanner package. Or, use an
appropriate shield or eye protector together with your scanner to avoid the
damage from the direct blue light to your eyes.
For details on how to use the supplied lter plate, refer to section of this
manual titled ”Using the Filter Plate”.

6 Bio-1000F User's Manual
Table of Contents
Preface ................................................................................................2
Safety Regulations ..............................................................................3
FCC Compliance Statement ................................................................ 3
Regulations ...................................................................................... 4
Safety Precautions ............................................................................ 5
Knowing about Your Gel scanner........................................................9
Features of the Bio-1000F.................................................................. 9
System Requirements ...................................................................... 10
Taking a Closer Look ....................................................................... 11
Front/Open View....................................................................... 11
Rear View ................................................................................ 12
Initial Setup .......................................................................................13
Step 1: Unpacking Package Contents ................................................ 14
Step 2: Installing the Software.......................................................... 15
Step 3: Unlocking the Scanner.......................................................... 16
Step 4: Connecting the Scanner........................................................ 18
To the Power Cord .................................................................... 18
To the Hi-Speed USB Cable ........................................................ 17
Digitizing a Gel..................................................................................18
Positioning a Gel ............................................................................. 18
Scanning a Gel ............................................................................... 19
Excising or Observing a Gel...............................................................21
Using the Filter Plate ....................................................................... 21
Knowing the MiBio Fluo Software Better ..........................................23
Launching MiBio Fluo....................................................................... 23
Exiting MiBio Fluo ........................................................................... 23
Main Control Panel .......................................................................... 24
Image Capture Button................................................................ 26
Light Up Button ........................................................................ 26
Settings Button......................................................................... 28
File's Saving Location .......................................................... 28
Gray Mode ......................................................................... 29
Resolution.......................................................................... 29
Stain Prole........................................................................ 29

Bio-1000F User's Manual 7
File Naming ........................................................................ 30
File Format......................................................................... 31
Show Warning Message(s) ................................................... 31
Care and Cleaning .............................................................................32
Cleaning the Glass Surfaces ............................................................. 32
Maintenance ......................................................................................33
Appendix ...........................................................................................34
Troubleshooting .............................................................................. 34
Specications ................................................................................. 35

8 Bio-1000F User's Manual

Bio-1000F User's Manual 9
Knowing about Your Gel scanner
The Bio-1000F is a gel scanner which is specically designed for the use of
scanning with global EtBr-alternative stain brands.
With a patented optical design, sensitive CCD and exible software, Bio-1000F
is capable to capture faint uorescence, which demonstrates more sensitive and
better performance than UV- and other blue-LED based gel imaging systems.
Besides, equipped with blue-LED and lter plate, Bio-1000F provides multilevel
protections from direct damages to eyes and skins, making safer and friendlier to
both researchers and environment.
Features of the Bio-1000F
The Bio-1000F comes with several important features, including the following:
• High sensitivity of uorescence — Bio-1000F goes perfectly with global EtBr-
alternative stain brands. The highest sensitivity of Bio-1000F can reach to
0.008 ng/μl.
• Space-saving compact design — The modern and compact design of the Bio-
1000F makes it perfect for small rooms and workshops, saving desk space
and keep storage space neat and clean.
• Hi-Speed USB connecting port — High-Speed USB (USB 2.0) is capable of
480 Mbps data transfer, provides plug-and-play connectivity for a hassle-free
installation.
• Microtek MiBio Fluo software — The easy and friendly user interface of
MiBio Fluo stores gel images in a computer readable format, offering an easy
and quick access and management.
• Easy to operate, clean and maintain — The Bio-1000F requires virtually no
routine maintenance and no daily cleaning, which is ideal for busy working
environments.

10 Bio-1000F User's Manual
System Requirements
In order to use your Bio-1000F, your computer must satisfy the following system
requirements:
• CD-ROM drive (for installing software)
• Color display with 24-bit color output capability
• 512 MB RAM or above
• 300 MB HDD or above
• Pentium IV PC or higher with Hi-Speed USB (USB 2.0)
• Microsoft Windows XP, Windows 7, Windows 8 or Windows 10

Bio-1000F User's Manual 11
Taking a Closer Look
Open View
No. Names of Parts Functions of Parts
1 Holes (2) Used to hang up a lter plate when lighting up a blue-LED
for a gel excising or observation.
2 Scanner Lid The top cover of the scanner.
3 Rear Glass Surface The place where the scanner makes a calibration.
4 Red Arrow Mark Used to align a gel properly.
5 Front Glass Surface The place to put a gel for scanning, excising or observing.

12 Bio-1000F User's Manual
Rear View
No. Names of Parts Functions of Parts
1 Hi-Speed USB Port Connects the Hi-Speed USB (USB 2.0) cable to the computer.
2 Power Connector Connects the scanner to the power adapter.
3 Power Switch Turns the scanner on or off.

Bio-1000F User's Manual 13
Initial Setup
In order to ensure that the scanner works properly, follow the three steps below
to set up your scanner.
1. Unpacking the Package Contents
2. Installing the Software
3. Unlocking the Scanner
4. Connecting the Scanner
NOTE: Before unpacking, inspect the shipping carton for any signs of
mishandling or damage rst. The scanner’s packing carton and padding
material have been carefully chosen to prevent damage to the unit in shipping
and can withstand a reasonable amount of pressure.
After unpacking your scanner package, refer to the later section to ensure
that you received all of the parts necessary for scanner setup. If there are any
damaged or missing parts, notify the shipper immediately or contact customer
service.
Save the carton and all packing materials. If you need to ship the scanner
later, it is recommended that you should repack it using the original wire ties,
plastic bags, foam supports and cartons, which protects your scanner from
unnecessary damage.

14 Bio-1000F User's Manual
Step 1: Unpacking Package Contents
After you have unpacked your scanner package, please check for the major
components listed below.
Scanner
Hi-Speed USB cable
Power cord
Software CD
Power adapter
Filter plate
Drivers & Software Upgrades
After you nish the installation of software, if you found that the installed drivers and software
cannot run your product or your computer system properly later, please go to the Microtek
Download Service site at ww7.microtek.com.tw/service.php to download and install any updates
you may require.
For additional information about Microtek products, please visit our website at www.microtek.com.

Bio-1000F User's Manual 15
Step 2: Installing the Software
Important: Do not remove the yellow stickers from your scanner until you are
told to do so. You must install all software before connecting your scanner.
Always close any open programs and turn off Anti-virus utilities before installing
software.
1. Turn on your computer.
2. Place the Microtek Software
CD/DVD into a drive.
3. Follow the on-screen
instructions to install the driver
and software.
NOTE: If the Microtek Software
Installer screen does not come
up automatically, double-click
the following in succession:
“My Computer”; the CD/DVD-
ROM icon; then cdsetup.exe to
start the installer program.
4. To install the software on the Microtek Software CD/DVD, click each
software program in the order that it appears on the screen to install, and
follow the on-screen instructions.
5. Click EXIT on the Microtek Software Installer screen when all the software
has been installed.
6. Restart your computer.
Drivers & Software Upgrades
After you nish the installation of software, if you found that the installed drivers and software
cannot run your product or your computer system properly later, please go to the Microtek
Download Service site at ww7.microtek.com.tw/service.php to download and install any updates
you may require.
For additional information about Microtek products, please visit our website at www.microtek.com.

16 Bio-1000F User's Manual
Step 4: Connecting the Scanner
To the Power adapter
1. Connect the power adapter to the back of the scanner.
2. Plug one end of the power cord into the power adapter, and plug the other
end of the power cord into a wall outlet or other power source.
Step 3: Unlocking the Scanner
1. With the scanner power off, tilt the scanner and locate the locking switch
at the bottom right corner of the scanner. Do not turn the scanner upside
down when attempting to unlock, as this may damage the scanner's
mechanism.
2. Push the locking switch to the position as indicated in the graphic, with the
icon on the lock showing as “unlocked”.
NOTE: To lock the scanner (for shipping and other purposes), turn the locking
switch to the “Locked” position. When locking the scanner, do not turn the
scanner upside down when locking the scanner back.
Unlocked Locked

Bio-1000F User's Manual 17
To the Hi-Speed USB Cable
3. Connect one end of the Hi-Speed USB cable to your scanner.
4. Connect the other end of the Hi-Speed USB cable to the computer’s USB
port.
Power switch
5. Turn the Power switch on the back of the scanner to “On” and immediate
the scanner is ready to scan.

18 Bio-1000F User's Manual
Digitizing a Gel
Positioning a Gel
1. Lift up the scanner lid.
2. Place a gel to be scanned on the scan bed. Use the red arrow mark at front
of the scanner to align the scanned gel at center.
3. Lower down the scanner lid.
Important: Make
sure that Calibration
window on the glass
surface is kept clear
and free of obstruction
at all times.
Use the red arrow
mark at front of the
scanner to align the gel
to be scanned properly
on the scan bed.
NOTE: When closing the scanner lid, make sure that it has been shut tightly;
otherwise, the light may leak and generate poor scan quality.

Bio-1000F User's Manual 19
Scanning a Gel
MiBio Fluo is a scanning software designed by Microtek for gel scanning. Its
simple and user-friendly interface enables you to easily start the scanning process
and nish all the scan jobs.
1. Follow the procedures for “Positioning a Gel” to place the gel to be scanned
on the scanner glass surface.
2. Launch MiBio Fluo by clicking the program icon on the desktop of the
computer.
3. Click the Image Capture button on the control panel to start scanning.
When done, the scanned images will be automatically saved into the folder
dened in Settings window. At the same time, the scanned images will be
displayed in thumbnail sizes in the Thumbnail View area and labeled with the
le name, grouped by a date, time and number.
Click the image in the Thumbnail View area and then it will be enlarged and
displayed in the Preview area.
Refer to the section “Knowing the MiBio Fluo Software Better” in this manual
for the details about how to change a folder or a name for a scanned image
in the Settings window.
4. If necessary, drag the bar of Exposure Time to adjust the exposure time for
a scanned gel, moving it to the left to decrease the exposure time and to
the right to increase on the contrary. Or, you can also click the Plus (+) or
Minus (-) button next to the bar to increase or decrease the exposure time.
When done, click the Image Capture button again. A new thumbnail-size
image will be displayed in the Thumbnail View area.
5. If you have to delete one piece of image in the Thumbnail View area, use
the mouse to select the image you want to delete and then click the Delete
button on the keyboard. To delete several images at one time, click and hold
on the Ctrl button on the keyboard; then, use the mouse to select images
you want to delete one by one and nally click the Delete button on the
keyboard.

20 Bio-1000F User's Manual
Thumbnail View area Preview area