Min NucleoSpin RNA Clean-up User manual

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RNA clean-up
User manual
NucleoSpin®RNA Clean-up
May 2014 / Rev. 05
A030383 / 1040.3

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RNA clean-up
MACHEREY-NAGEL – 05 / 2014, Rev. 05
Table of contents
1 Components 4
1.1 Kit contents 4
1.2 Reagents, consumables, and equipment to be supplied by user 5
1.3 About this user manual 5
2 Product description 6
2.1 The basic principle 6
2.2 Kitspecications 6
2.3 Handling, preparation, and storage of starting materials 7
2.4 Elution procedures 8
3 Storage conditions and preparation of working solutions 9
4 Safety instructions 10
5 Protocols 12
5.1 RNA Clean-up 12
5.2 RNA isolation from up to 105cells 14
6 Appendix 16
6.1 Troubleshooting 16
6.2 Ordering information 18
6.3 Product use restriction / warranty 19

RNA clean-up
MACHEREY-NAGEL – 05 / 2014, Rev. 05
4
1 Components
1.1 Kit contents
NucleoSpin®RNA Clean-up
10 preps 50 preps 250 preps
REF 740948.10 740948.50 740948.250
Lysis Buffer RA1 10 mL 25 mL 125 mL
Wash Buffer RA2 15 mL 15 mL 80 mL
Wash Buffer RA3
(Concentrate)*
6 mL 12 mL 3 x 25 mL
RNase-free H2O 13 mL 13 mL 60 mL
NucleoSpin®RNA Clean-up
Columns (light blue rings –
plus Collection Tubes)
10 50 250
Collection Tubes (2 mL) 10 50 250
Collection Tubes (1.5 mL) 10 50 250
User manual 1 1 1
* For preparation of working solutions and storage conditions see section 3.

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MACHEREY-NAGEL – 05 / 2014, Rev. 05
1.2 Reagents, consumables, and equipment to be supplied
by user
Reagents
• 96–100 % ethanol (to prepare Wash Buffer RA3 and to adjust RNA binding
conditions)
Consumables
• 1.5 mL microcentrifuge tubes
• Sterile RNase-free tips
Equipment
• Manual pipettors
• Centrifuge for microcentrifuge tubes
• Personal protection equipment (e.g., lab coat, gloves, goggles)
1.3 About this user manual
It is strongly recommended reading the detailed protocol sections of this user manual
if the NucleoSpin®RNA Clean-up kit is used for the rst time. Experienced users,
however, may refer to the Protocol-at-a-glance instead. The Protocol-at-a-glance is
designed to be used only as a supplemental tool for quick referencing while performing
thepuricationprocedure.
All technical literature is available on the internet at www.mn-net.com.
Please contact Technical Service regarding information about changes of the current
user manual compared to previous revisions.

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MACHEREY-NAGEL – 05 / 2014, Rev. 05
6
2 Product description
2.1 The basic principle
One of the most important aspects in the isolation and handling of RNA is to prevent
degradation of the RNA during the isolation procedure. With the NucleoSpin®RNA
Clean-up kit, RNA containing samples are mixed with a solution containing large
amounts of chaotropic ions. This solution immediately inactivates RNases – which are
present in virtually all biological materials – and creates appropriate binding conditions
which favor adsorption of RNA to the silica membrane. Simple washing steps remove
salts, metabolites, organics like phenol, and macromolecular cellular components.
PureRNAisnallyelutedunderlowionicstrengthconditionswithRNase-freewater
(supplied).
The RNAclean-up preparation using NucleoSpin®RNA Clean-up kits can be performed
at room temperature. The eluate, however, should be treated with care because RNA
is very sensitive to trace contaminations of RNases, often found on general lab ware,
ngerprintsanddust.ToensureRNAstabilitykeepRNAfrozenat-20°Cforshort-term
or-70°Cforlong-termstorage.
2.2 Kit specications
• NucleoSpin®RNA Clean-up kits are ideal for the clean-up of total RNA from
RNA preparations which contain inacceptable amounts of RT-PCR inhibitors
(e.g., RNA prepared with phenol-chloroform based methods).
• The kit is further recommended for the isolation of RNA from small amounts of
culturedcellswhenevercopuricationofsomegenomicDNAisacceptabel.The
kitsallowpuricationofpureRNAwithanA260/A280 ratio generally exceeding 1.9
(measured in TE buffer (pH 7.5)).
• NucleoSpin®RNA Clean-up kits are recommended for the clean-up of RNA
fromenzymaticreactionslikein vitrotranscribedRNA,amplicationreactions,
biotinylatedRNA,oruorescent(Cydye)labeledRNA.
• The puried RNA is ready to use for applications like enzymatic labelling
reactions (e.g., dye incorporation), reverse transcriptase-PCR (RT-PCR), and
for DNA/RNA based chip hybridisations (e.g., MWG rat microarray, MWG,
Ebersberg,GermanyorHumanGenomeU133AArray,Affymetrix,USA).
• IntegrityofpuriedRNA,originallyisolatedfromforexampleeukaryoticcells,
is examined by denaturing agarose gel electrophoresis: rRNA bands are sharp,
with the 28S band being about twice as intense as the 18S band.
• Thestandardprotocol(section5.1)allowstheclean-upofupto200μgofRNA
per NucleoSpin®RNA Clean-up Column or the isolation of total RNA from up to
1 x 105cultured cells (section 5.2).

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MACHEREY-NAGEL – 05 / 2014, Rev. 05
Table 1: Kit specications at a glance
Parameter NucleoSpin®RNA Clean-up
Technology Silica-membrane technology
Format Mini spin columns
Sample material <100μLRNAsamplewithsinglecolumnloading
containingupto200μgRNA
<200μLRNAsamplewithdoublecolumnloading
containingupto200μgRNA
Up to 105cells
Fragmentsize > 200 nt
Typical recovery
(0.1–200μgRNAinput)
85–95 %
A260/A280 1.9–2.1
Elution volume 40–120μL
Preparation time Approx. 20 min/6 preps
Binding capacity 200μg
2.3 Handling, preparation, and storage of starting materials
RNA intended to be used as sample for the NucleoSpin®RNA Clean-up procedure
shouldbehandledwiththesamecareasanyRNAsample.Thestabilityofprepuried
RNA samples (e.g., RNA isolated with phenol based protocols) depends very much on
the performed procedure. RNA in biological samples is not protected against digestion
untilthesamplematerialisashfrozenordisruptedinthepresenceofRNaseinhibiting
ordenaturingagents.Thereforeitisimportantthatbiologicalsamplesareashfrozenin
liquid N2immediatelyandstoredat-70°Corprocessedassoonaspossible.Samples
canbestoredinlysisbufferafterdisruptionat-70°Cforuptooneyear,at+4°Cfor
upto24hoursoruptoseveralhoursatroomtemperature.Frozensamplesarestable
upto6months.Frozensamplesinlysisbuffershouldbethawedslowlybeforestarting
with the isolation of total RNA.
Wear gloves at all times during the preparation. Change gloves frequently.

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MACHEREY-NAGEL – 05 / 2014, Rev. 05
8
2.4 Elution procedures
It is possible to adjust the elution method and the volume of RNase-free water used for
the subsequent application of interest. In addition to the standard method described in
theindividualprotocols(recoveryrateabout70–90%)thereareseveralmodications
possible:
• High yield: Perform two elution steps with the volume indicated in the individual
protocol. About 90–100 % of bound nucleic acid will be eluted.
• High yield and high concentration: Elute with the standard elution volume
and apply the eluate once more onto the column for reelution.
Eluted RNA should immediately be placed and always kept on ice for optimal stability
becausealmostomnipresentRNases(generallabware,ngerprints,dust)willdegrade
RNA.Forshort-termstoragefreezeat-20°C,forlong-termstoragefreezeat-70°C.

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MACHEREY-NAGEL – 05 / 2014, Rev. 05
3 Storage conditions and preparation of working
solutions
Attention: Buffers RA1 and RA2 contain chaotropic salt. Wear gloves and goggles!
CAUTION: Buffers RA1 and RA2 contain guanidinium thiocyanate which can form
highly reactive compounds when combined with bleach (sodium hypochlorite). DO
NOT add bleach or acidic solutions directly to the sample-preparation waste.
• Allkitcomponentsshouldbestoredatroomtemperature(18–25°C)andare
stable up to one year. Storage at lower temperatures may cause precipitation
of salts.
• Check that 96–100 % ethanol is available as additional solution in the lab.
Before starting any NucleoSpin®RNA Clean-up protocol, prepare the following:
• Wash Buffer RA3: Add the indicated volume of 96–100 % ethanol (see table
below) to Wash Buffer RA3 Concentrate. Mark the label of the bottle to indicate
that ethanol was added. Store Wash Buffer RA3 at room temperature (18–
25°C)foruptooneyear.
NucleoSpin®RNA Clean-up
10 preps 50 preps 250 preps
REF 740948.10 740948.50 740948.250
Wash Buffer RA3
(Concentrate)
6 mL
Add 24 mL ethanol
12 mL
Add 48 mL ethanol
3 x 25 mL
Add 100 mL
ethanol
to each bottle

MACHEREY-NAGEL – 05 / 2014, Rev. 0510
RNA clean-up
4 Safety instructions
The following components of the NucleoSpin®RNA Clean-upkitscontainhazardous
contents.
Wear gloves and goggles and follow the safety instructions given in this section.
GHS classication
Only harmful features do not need to be labeled with H and P phrases up to 125 mL
or 125 g.
Mindergefährliche Eigenschaften müssen bis 125 mL oder 125 g nicht mit H- und P-Sätzen gekennzeichnet
werden.
Component Hazard contents GHS symbol Hazard
phrases
Precaution
phrases
Inhalt Gefahrstoff GHS Symbol H-Sätze P-Sätze
RA1 Guanidiniumthiocyanate
30–60 %
Warning 302, 412,
EUH031
260, 273,
301+312,330
Guanidiniumthiocyanat
30–60 %
Achtung
RA2 Guanidiniumthiocyanate
30–60%+ethanol20-
35 %
Warning 226, 302,
412,
EUH031
210, 233, 260,
273,301+312,
330,403+235
Guanidiniumthiocyanat
30–60 % + Ethanol 20–35 %
Achtung
Hazard phrases
H 226 Flammable liquid and vapour.
Flüssigkeit und Dampf entzündbar.
H 302 Harmful if swallowed.
Gesundheitsschädlich bei Verschlucken.
H 412 Harmful to aquatic life with long lasting effects.
Schädlich für Wasserorganismen, mit langfristiger Wirkung.
EUH031 Contact with acids liberates toxic gas.
Entwickelt bei Berührung mit Säure giftige Gase.
Precaution phrases
P 210 Keepawayfromheat,hotsurfaces,sparks,openamesandotherignition
sources. No smoking.
Von Hitze, heißen Oberächen, Funken, offenen Flammen sowie anderen
Zündquellenarten fernhalten. Nicht rauchen.
P 233 Keep container tightly closed
Behälter dicht verschlossen halten.
P 260 Donotbreathevapours.
Dampf nicht einatmen.
P 273 Avoid release to the environment.
Freisetzung in die Umwelt vermeiden.
P301+312 IFSWALLOWED:CallaPOISONCENTER/doctor/…/ifyoufeelunwell.
BEI VERSCHLUCKEN: Bei Unwohlsein GIFTINFORMATIONSZENTRUM / Arzt /…
anrufen.

11MACHEREY-NAGEL – 05 / 2014, Rev. 05
Precaution phrases
P 330 Rinse mouth.
Mund ausspülen.
Bei Symptomen der Atemwege: GIFTINFORMATIONSZENTRUM oder Arzt anrufen.
P403+235 Store in a well ventilated place. Keep cool.
Kühl an einem gut belüfteten Ort aufbewahren.
ForfurtherinformationpleaseseeMaterialSafetyDataSheets (www.mn-net.com).
Weiterführende Informationen nden Sie in den Sicherheitsdatenblättern (www.mn-net.com).
RNA clean-up

MACHEREY-NAGEL – 05 / 2014, Rev. 0512
5 Protocols
5.1 RNA Clean-up
Before starting the preparation:
• Check if Wash Buffer RA3 was prepared according to section 3.
1 Sample preparation
FillupRNAsamplessmallerthan100μLwithRNase-free
water to 100 μL.
RNAsamplesfrom100–200μLshouldbelledupwith
RNase-freewaterto200μL.
Fill up RNA
sample to
100 μL with
water
2 Preparation of lysis-binding buffer premix
Prepare a Buffer RA1-ethanol premix with a ratio of 1:1.
For each 100 μL RNA sample mix 300 μL Buffer RA1
and 300 μL of ethanol (96–100 %).
If multiple samples are processed, the preparation of a
master-premixisrecommended(e.g.,2mLBufferRA1+
2mL98 %ethanolforapproximately6preparations).
Prepare
premix:
Mix
300 μL RA1
with
300 μL ethanol
(96–100 %)
3 Adjust RNA binding conditions
To 100 μL RNA sample add 600 μL (6 volumes) of
Buffer RA1-ethanol-premix. Mix sample with premix by
vortexing.
If a 200 μL RNA sample is processed, add 1200 μL
Buffer RA1-ethanol premix.
After addition of ethanol a stringy precipitate may become
visible which will not affect the RNA isolation. Be sure to mix
thouroughly and apply sample as homogeneous solution
onto the column.
+ 6 vol.
premix
Mix
NucleoSpin®RNA Clean-up

13MACHEREY-NAGEL – 05 / 2014, Rev. 05
4 Bind RNA
For each preparation, take one NucleoSpin®RNA Clean-
up Column (light blue ring) placed in a Collection Tube
andloadthelysate(700μL).
Centrifuge for 30 s at 8,000 x g. Discard Collection
Tube with ow-through and place the column in a new
Collection Tube.
Maximal loading capacity of NucleoSpin®RNA Clean-up
Columns is 750 μL. Repeat the procedure if larger volumes
are to be processed.
Load 700 μL
lysate
8,000 x g,
30 s
5 Wash and dry silica membrane
1st wash
Add 700 μL Buffer RA3 to the NucleoSpin®RNA Clean-
up Column. Centrifuge for 30 s at 8,000 x g. Discard
ow-throughandreuseCollectionTube.
+ 700 μL RA3
8,000 x g,
30 s
2nd wash
Add 350 μL Buffer RA3 to the NucleoSpin®RNA Clean-
up Column. Centrifuge for 2 min at 8,000 x g.
Transfer the NucleoSpin®RNA Clean-up Column to a
nuclease-free Collection Tube (1.5 mL, supplied). Open
the lid of the column and let the membrane dry for 3 min.
If for any reason, the liquid level in the Collection Tube
has reached the NucleoSpin®RNA Clean-up Column after
centrifugation, discard ow-through and centrifuge again.
The procedure ensures complete removal of ethanol from
the column.
+ 350 μL RA3
8,000 x g,
2 min
6Elute RNA
Elute the RNA in 60 μL RNase-free H2O(supplied) and
centrifuge at 8,000 x gfor 1 min.
If higher RNA concentrations are desired, elution can be
done with 40 μL. Overall yield, however, will decrease when
using smaller volumes.
For further alternative elution procedures see section 2.4.
+ 60 μL
RNase-free
H2O
8,000 x g,
1 min
NucleoSpin®RNA Clean-up

MACHEREY-NAGEL – 05 / 2014, Rev. 0514
5.2 RNA isolation from up to 105cells
Before starting the preparation:
• Check if Wash Buffer RA3 was prepared according to section 3.
1 Sample preparation
As sample material use up to 105cells in a volume of
up to 100 μL.
Fill up sample
to 100 μL
(e.g. with PBS)
2 Cell lysis
Add 300 μL Buffer RA1 and vortex vigorously in order to
lyse the cells.
+ 300 μL RA1
Vortex
3 Adjust RNA binding conditions
Add 300 μL ethanol (96 – 100 %) to the lysate and mix
by vortexing or pipetting up and down.
After addition of ethanol a stringy precipitate may become
visible which will not affect the RNA isolation. Be sure to mix
thoroughly and apply sample as homogeneous solution onto
the column.
+ 300 μL
ethanol
(96–100 %)
Mix
4 Bind RNA
For each preparation, take one NucleoSpin®RNA Clean-
up Column (light blue) placed in a Collection Tube and
loadthelysate(700μL).
Centrifuge for 30 s at 8,000 x g. Discard Collection
Tube with ow-through and place the column in a new
Collection Tube.
Maximal loading capacity of NucleoSpin®RNA Clean-up
Columns is 750 μL. Repeat the procedure if larger volumes
are to be processed.
Load lysate
8,000 x g,
30 s
NucleoSpin®RNA Clean-up

15MACHEREY-NAGEL – 05 / 2014, Rev. 05
NucleoSpin®RNA Clean-up
5 Wash and dry silica membrane
1st wash
Add 250 μL Buffer RA2 to the NucleoSpin®RNA Clean-
up Column. Centrifuge for 30 s at 8,000 x g. Discard
ow-throughandreuseCollectionTube
+ 250 μL RA2
8,000 x g,
30 s
2nd wash
Add 700 μL Buffer RA3 to the NucleoSpin®RNA Clean-
up Column. Centrifuge for 30 s at 8,000 x g. Discard
ow-throughandreuseCollectionTube.
+ 700 μL RA3
8,000 x g,
30 s
3rd wash
Add 350 μL Buffer RA3 to the NucleoSpin®RNA Binding
Column. Centrifuge for 2 min at 8,000 x g.
Transfer the NucleoSpin®RNA Clean-up Column to a
nuclease-free Collection Tube (1.5 mL, supplied). Open
the lid of the column and let the membrane dry for 3 min.
If for any reason, the liquid level in the Collection Tube
has reached the NucleoSpin®RNA Clean-up Column after
centrifugation, discard ow-through and centrifuge again.
The procedure ensures complete removal of ethanol from
the column.
+ 350 μL RA3
8,000 x g,
2 min
6Elute RNA
Elute the RNA in 60 μL RNase-free H2O, (supplied) and
immediately centrifuge at 8,000 x g. for 1 min.
If higher RNA concentrations are desired, elution can be
done with 40 μL. Overall yield, however, will decrease when
using smaller volumes.
For further alternative elution procedures see section 2.4.
+ 60 μL
RNase-free
H2O
8,000 x g,
1 min

MACHEREY-NAGEL – 05 / 2014, Rev. 0516
6 Appendix
6.1 Troubleshooting
Problem Possible cause and suggestions
RNA is
degraded / no
RNA obtained
RNase contamination
• Create an RNase-free working environment. Wear
gloves during all steps of the procedure. Change gloves
frequently. Use of sterile, disposable polypropylene tubes is
recommended. Keep tubes closed whenever possible during
thepreparation.Glasswareshouldbeoven-bakedforatleast
2hoursat250°Cbeforeuse.
Poor RNA
quality or yield
Reagents not applied or restored properly
• Sample and reagents have not been mixed completely.
Always vortex vigorously after each reagent has been added.
• No ethanol has been added. Binding of RNA to the silica
membrane is only effective in the presence of ethanol.
Kit storage
• Store kit components at room temperature. Storage at low
temperatures may cause salt precipitation.
• Keep bottles tightly closed in order to prevent evaporation or
contamination.
Sample material
• Sample material not stored properly. Whenever possible, use
freshmaterial.Ifthisisnotpossible,ashfreezethesamples
in liquid N2.Samplesshouldalwaysbekeptat-70 °C.Never
allow tissues to thaw before addition of lysis buffer. Perform
disruption of samples in liquid N2.
Contamination
of RNA with
genomicDNA
• The NucleoSpin®RNA Clean-up procedure does not
comprise a DNA digestion step. Therefore the extent of
DNAcontaminationmainlydependsonthesamplematerial.
Iflowestlevel of DNA contamination isdesired,use oneof
therDNasecontainingNucleoSpin®RNA kits (see ordering
information).
RNA Clean-up

17
RNA clean-up
MACHEREY-NAGEL – 05 / 2014, Rev. 05
Problem Possible cause and suggestions
Suboptimal
performance
of RNA in
downstream
experiments
Carry-over of ethanol or salt
• Donotlettheow-throughtouchthecolumnoutletafterthe
second wash using Wash Buffer RA3. Be sure to centrifuge
at the corresponding speed for the respective time in order to
remove ethanolic Wash Buffer RA3 completely.
• Check if Wash Buffer RA3 has been equilibrated to room
temperature before use. Washing at lower temperatures
lowersefciencyofsaltremovalbyWashBufferRA3.
• A 2 min centrifugation with a subsequent 3 min drying with
openlidissufcentforanextensiveremovalofethanolfrom
the column. Residual ethanol will typically be around 1 %.
Increasing the drying step with open lid from 3 min to 20 min
will decrease the residual ethanol content commonly to below
0.1 %, but also RNA recovery will be reduced 5–20 %.
Store isolated RNA properly
• Eluted RNA should always be kept on ice for optimal stability
since trace contaminations of omnipresent RNases (general
lab ware, ngerprints, dust) will degrade the isolated RNA.
Forshorttermstoragefreezeat-20°C,forlongtermstorage
freezeat-70°C.
RNA concentration is too low
• For highest RNA concentration and most sensitive
downstream applications, NucleoSpin®RNA Clean-up XS is
recommended. NucleoSpin®RNA Clean-up XS allows elution
inonly5–20μLvolume(seeorderinginformation).
Higher RNA
yield than
theoretically
possible
• If performing clean-up of samples containing less than
approximately300ng,RNAsubsequentquanticationbyA260
measurement may simulate yields larger than the RNA input.
This may be due to absorbance of silica abrasion. In order to
prevent incorrect A260 quantication of small RNA amounts,
centrifuge the elution tube for 30 s at 8.000–11.000 x gand
withdraw an aliquot for measurement without disturbing
any sediment or use a silica abrasion insensitive RNA
quanticationmethod(e.g.,RiboGreenuorescentdye).

MACHEREY-NAGEL – 05 / 2014, Rev. 0518
6.2 Ordering information
Product REF Pack of
NucleoSpin®RNA Clean-up 740948
740948
740948
.10
.50
.250
10
50
250
preps
preps
preps
NucleoSpin®RNA Clean-up XS 740903
740903
740903
.10
.50
.250
10
50
250
preps
preps
preps
NucleoSpin®RNA 740955
740955
740955
.10
.50
.250
10
50
250
preps
preps
preps
NucleoSpin®RNA XS 740902
740902
740902
.10
.50
.250
10
50
250
preps
preps
preps
NucleoSpin®RNA Midi 740962.20 20 preps
NucleoSpin®RNA Blood 740200
740200
.10
.50
10
50
preps
preps
NucleoSpin®miRNA 740971
740971
740971
.10
.50
.250
10
50
250
preps
preps
preps
NucleoSpin®RNA Plant 740949
740949
740949
.10
.50
.250
10
50
250
preps
preps
preps
NucleoSpin®totalRNA FFPE 740982
740982
740982
.10
.50
.250
10
50
250
preps
preps
preps
NucleoSpin®totalRNA FFPE XS 740969
740969
740969
.10
.50
.250
10
50
250
preps
preps
preps
NucleoSpin®8 RNA 740698
740698 .5
12 x 8
60 x 8
preps
preps
NucleoSpin®96 RNA 740709
740709
740709
.2
.4
.24
2 x 96
4 x 96
24 x 96
preps
preps
preps
NucleoMag®96 RNA 744350
744350
.1
.4
1 x 96
4 x 96
preps
preps
RNA Clean-up

19MACHEREY-NAGEL – 05 / 2014, Rev. 05
Product REF Pack of
NucleoSpin®TriPrep* 740966
740966
740966
.10
.50
.250
10
50
250
preps
preps
preps
NucleoSpin®RNA/Protein 740933
740933
740933
.10
.50
.250
10
50
250
preps
preps
preps
Buffer RA1 740961
740961 .500
50
500
mL
mL
rDNaseSet 740963 1 set
Visit www.mn-net.com for more detailed product information.
6.3 Product use restriction / warranty
NucleoSpin®RNA Clean-up kit components are intended, developed, designed, and
sold FOR RESEARCH PURPOSES ONLY, except, however, any other function of the
productbeingexpresslydescribedinoriginalMACHEREY-NAGELproductleaets.
MACHEREY-NAGEL products are intended for GENERAL LABORATORY USE
ONLY!MACHEREY-NAGELproductsaresuitedforQUALIFIEDPERSONNELONLY!
MACHEREY-NAGEL products shall in any event only be used wearing adequate
PROTECTIVE CLOTHING. For detailed information please refer to the respective
Material Safety Data Sheet of the product! MACHEREY-NAGEL products shall
exclusively be used in anADEQUATE TEST ENVIRONMENT. MACHEREY-NAGEL
does not assume any responsibility for damages due to improper application of our
products in other elds of application.Application on the human body is STRICTLY
FORBIDDEN.Therespectiveuserisliableforanyandalldamagesresultingfromsuch
application.
DNA/RNA/PROTEINpuricationproductsofMACHEREY-NAGELaresuitableforIN-
VITRO-USES ONLY!
ONLYMACHEREY-NAGELproductsspeciallylabeledasIVDarealsosuitableforIN-
VITRO-diagnostic use. Please pay attention to the package of the product. IN-VITRO-
diagnosticproductsareexpresslymarkedasIVDonthepackaging.
IF THERE IS NO IVD SIGN,THE PRODUCT SHALL NOT BE SUITABLE FOR IN-
VITRO-DIAGNOSTICUSE!
ALL OTHER PRODUCTS NOT LABELED AS IVD ARE NOT SUITED FOR ANY
CLINICALUSE(INCLUDING,BUTNOTLIMITEDTODIAGNOSTIC,THERAPEUTIC
AND/ORPROGNOSTICUSE).
RNA Clean-up
* DISTRIBUTION AND USE OF NUCLEOSPIN® TRIPREP IN THE USA IS PROHIBITED FOR PATENT
REASONS.

RNA clean-up
MACHEREY-NAGEL – 05 / 2014, Rev. 05
20
No claim or representations is intended for its use to identify any specic organism
or for clinical use (included, but not limited to diagnostic, prognostic, therapeutic, or
blood banking). It is rather in the responsibility of the user or - in any case of resale of
the products - in the responsibility of the reseller to inspect and assure the use of the
DNA/RNA/proteinpuricationproductsofMACHEREY-NAGELforawell-denedand
specicapplication.
MACHEREY-NAGELshallonlyberesponsiblefortheproductspecicationsandthe
performancerangeofMNproductsaccordingtothespecicationsofin-housequality
control, product documentation and marketing material.
ThisMACHEREY-NAGELproductisshippedwithdocumentationstatingspecications
and other technical information. MACHEREY-NAGEL warrants to meet the stated
specications.MACHEREY-NAGEL´ssoleobligationandthecustomer´ssoleremedy
is limited to replacement of products free of charge in the event products fail to perform
as warranted. Supplementary reference is made to the general business terms and
conditionsofMACHEREY-NAGEL,whichareprintedonthepricelist.Pleasecontact
us if you wish to get an extra copy.
ThereisnowarrantyforandMACHEREY-NAGELisnotliablefordamagesordefects
arising in shipping and handling (transport insurance for customers excluded), or
out of accident or improper or abnormal use of this product; defects in products or
components not manufactured by MACHEREY-NAGEL, or damages resulting from
suchnon-MACHEREY-NAGELcomponentsorproducts.
MACHEREY-NAGEL makes no other warranty of any kind whatsoever, and
SPECIFICALLY DISCLAIMS AND EXCLUDES ALL OTHER WARRANTIES OF
ANY KIND OR NATURE WHATSOEVER, DIRECTLY OR INDIRECTLY, EXPRESS
OR IMPLIED, INCLUDING, WITHOUT LIMITATION, AS TO THE SUITABILITY,
REPRODUCTIVITY, DURABILITY, FITNESS FOR A PARTICULAR PURPOSE OR
USE,MERCHANTABILITY,CONDITION,ORANYOTHERMATTERWITHRESPECT
TOMACHEREY-NAGELPRODUCTS.
In no event shall MACHEREY-NAGEL be liable for claims for any other damages,
whether direct, indirect, incidental, compensatory, foreseeable, consequential, or
special(includingbutnotlimitedtolossofuse,revenueorprot),whetherbasedupon
warranty, contract, tort (including negligence) or strict liability arising in connection with
thesaleorthefailureofMACHEREY-NAGELproductstoperforminaccordancewith
thestatedspecications.ThiswarrantyisexclusiveandMACHEREY-NAGELmakes
no other warranty expressed or implied.
The warranty provided herein and the data, specications and descriptions of this
MACHEREY-NAGELproductappearinginMACHEREY-NAGELpublishedcatalogues
and product literature are MACHEREY-NAGEL´s sole representations concerning
the product and warranty. No other statements or representations, written or oral, by
MACHEREY-NAGEL´semployees,agentorrepresentatives,exceptwrittenstatements
signedbyadulyauthorizedofcerofMACHEREY-NAGELareauthorized;theyshould
not be relied upon by the customer and are not a part of the contract of sale or of this
warranty.
Product claims are subject to change. Therefore please contact our Technical Service
Team for the most up-to-date information on MACHEREY-NAGEL products. You
mayalso contactyourlocaldistributorfor generalscientic information.Applications

21
RNA clean-up
MACHEREY-NAGEL – 05 / 2014, Rev. 05
mentionedinMACHEREY-NAGELliterature areprovided for informationalpurposes
only.MACHEREY-NAGELdoesnotwarrantthatallapplicationshavebeentestedin
MACHEREY-NAGELlaboratoriesusingMACHEREY-NAGELproducts. MACHEREY-
NAGELdoesnotwarrantthecorrectnessofanyofthoseapplications.
Last updated: 07 / 2010, Rev. 03
Please contact:
MACHEREY-NAGELGmbH&Co.KG
Tel.:+492421969-270
[email protected]