Monmouth Scientific PCR650 Operating instructions

ISSUE C 26
th
Sept 2008
PCR650
OPERATING AND MAINTENANCE MANUAL

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CONTENTS
SECTION 1 ........................................................................ 4
DESCRIPTION OF THE CABINET ............................................................... 4
SECTION 2 ........................................................................ 5
INSTALLATION............................................................................................. 5
ASSEMBLY.......................................................................................................... 5
TESTING / COMMISSIONING..............................................................................5
SECTION 3 ........................................................................ 6
OPERATION.................................................................................................. 6
STARTING THE CABINET...................................................................................6
UV CYCLE OPERATION......................................................................................6
STARTING A UV STERILISATION CYCLE .........................................................6
UV TIMER ALARM...............................................................................................7
LOW AIRFLOW ALARM ......................................................................................7
SYSTEM MONITOR .............................................................................................7
SECTION 4 ........................................................................
FILTERS........................................................................................................
PRE-FILTER - CHANGING ..................................................................................
MAIN HEPA FILTER - CHANGING......................................................................
SECTION 5 ........................................................................ 9
MAINTENANCE ............................................................................................ 9
FUSES.................................................................................................................. 9
LIGHTING ............................................................................................................ 9
UV TUBE.............................................................................................................. 9
AIRFLOW............................................................................................................. 9
LOW AIRFLOW ALARM ......................................................................................9
CIRCUIT DIAGRAM ........................................................................................... 10
SECTION 6 ...................................................................... 11
SERVICING ................................................................................................. 11
Warning
This system must be used in compliance with these instructions and any
repairs or maintenance carried out by qualified personnel.
For parts or service information please contact Monmouth Scientific on:
[phone]

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SECTION 1
DESCRIPTION OF THE CABINET
The Monmouth PCR Cabinet provides optimum clean air characteristics or the
processing o Polymerase Chain Reaction (PCR).
The cabinet signi icantly lowers the risk o cross contamination o your PCR
sample by providing a curtain o Class 100 clean air (Laminar Flow) over the
entire worksur ace. To urther reduce the risk o cross contamination between
samples, the PCR cabinet is supplied with a time controlled UV light to sterilise
the working area between samples. 6 individual pre-set time unctions are
provided as standard.
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Cabinet
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External
Dimensions
650mm Wide
550mm Deep
900mm High
Internal
Dimensions
630mm Wide
500mm Deep
500mm High

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SECTION 2
INSTALLATION
• The cabinet should be sited in a draught ree position
ASSEMBLY
The cabinet is supplied ully assembled with the main HEPA ilter and pre- ilter
itted.
TESTING / COMMISSIONING
A test certi icate will be supplied or con ormity to CE marking, electrical test
and air low.

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SECTION 3
OPERATION
STARTING THE CABINET
The cabinet is switched on by the green rocker switch to the right o the control
panel. I the sa ety screen is itted the luorescent light will come on and the an
will remain o . The an will start when the sa ety screen is removed.
UV CYCLE OPERATION
TO START A UV CYCLE THE SAFETY SCREEN MUST BE IN POSITION.
STARTING A UV STERILISATION CYCLE
Fit the Sa ety Screen and turn the cabinet on.
Press the UV button on the membrane panel to select the duration o the cycle.
• 1 Press = 5 minutes
• 2 Presses = 10 minutes
• 3 Presses = 15 minutes
• 4 Presses = 20 minutes
• 5 Presses = 25 minutes
• 6 Presses = 30 minutes
The UV pre-set timed cycle will start, the luorescent light will switch o and the
UV lamp will switch on. The red UV warning light on the membrane panel will
lash to indicate the cycle selected – (1 lash = 5 minutes, 2 lashes = 10
minutes etc.)
When the UV timing cycle is inished, the UV light will switch o , the UV
warning light will lash slowly and the audio will beep every 4 seconds to in orm
the cycle has inished.
A cycle may be interrupted at any time by pressing and holding the UV button.
The audio beep will stop and the cabinet will return to normal mode.

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I the night door is removed during the UV cycle, the unction will be cancelled.
The UV light will switch o , the an and luorescent light will switch on and the
cabinet will return to normal mode ready or use.
UV TIMER ALARM
The UV timer is pre-set to alarm a ter a 1000 hrs o tube operation. The tube
should be replaced at this interval. Although the blue light will still be visible the
UV power rom the tube will be diminished.
When the tube operation exceeds the pre-set limit, during a UV cycle an audio
beep will be observed every minute.
To reset the alarm timer, switch the cabinet o , press and hold the “LIGHT “
and the “ UV “ button and switch the cabinet on again. When an audio beep
sounds release the buttons. The counter will be reset and the system return to
normal mode.
LOW AIRFLOW ALARM
The air low is continuously monitored while the cabinet is running. An audible
alarm will sound i the air low drops below a preset level and the red low air low
warning light will lash on the control panel. This is an indication that the Pre-
Filter requires replacement – see section 4. I changing the pre- ilter does not
cancel the alarm the cabinet requires re-calibration or a new main HEPA ilter.
SYSTEM MONITOR
The UV timer control circuit has a monitoring component which will indicate a
system ailure. All red warning lights will lash on and o , a continuous audio
alarm will sound and the luorescent light will be switched o .
I this occurs the cabinet is unsa e to use. Contact Monmouth Scienti ic or
urther advice.
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SECTION 4
FILTERS
PRE-FILTER - CHANGING
The Pre-Filter should be changed when the low air low alarm is activated.
Remove the thumbscrews on top o the cabinet and li t o the pre- ilter
retainer. Replacement pre- ilters may be obtained rom Monmouth Scienti ic –
Part No PF-0023
MAIN HEPA FILTER - CHANGING
The HEPA ilter should be changed by trained personnel. Contact Monmouth
Scienti ic or advice.

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SECTION 5
MAINTENANCE
The cabinet should be isolated from the electricity supply before carrying
out any maintenance procedures.
FUSES
Always replace fuses with the correct type and rating:
The main uses (5A Type T) are located in the mains inlet socket at the back o
the cabinet. Remove the mains lead and withdraw the uses using a small
screwdriver.
The an use (3.15A Type T) is located on the control circuit board and may be
changed by removing the two securing screws rom inside the cabinet and
hinging the control panel open.
LIGHTING
The luorescent light tube can be accessed rom inside the cabinet. The starter
is located inside the control panel and may be changed by removing the two
securing screws rom inside the cabinet and hinging the control panel open.
The luorescent light use (2A Type F) is located on the control circuit board.
UV TUBE
The UV tube can be accessed rom inside the cabinet. The starter is located
inside the control panel and may be changed by removing the two securing
screws rom inside the cabinet and hinging the control panel open. The UV
light use (2A Type F) is located on the control circuit board.
AIRFLOW
WARNING: This procedure requires adjustment o live circuits and should only
be carried out by a quali ied person.
The air low should be set using a calibrated rotating vane anemometer. The
an speed is adjusted by turning the blue potentiometer on the speed control
circuit board inside the control panel. The speed should be set to achieve a
down low o 0.3 – 0.35m/sec. at 100mm above the lower edge o the ront ixed
glazing panel.
LOW AIRFLOW ALARM
1. Set the air low to the correct speed with new pre- ilters itted.
2. Switch the cabinet on and place a piece o paper or similar material against
the pre- ilter inlet grille covering all but 14 o the 84 holes to simulate a
partially blocked ilter.
3. Unscrew the pressure switch adjusting screw until the low air low warning
LED comes on. Screw in until the LED goes out then slowly unscrew until
the LED comes on again.
4. Remove and replace the paper several times to check operation o warning
LED / audio.

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CIRCUIT DIAGRAM

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SECTION 6
SERVICING
An annual service is recommended and will include the ollowing points:
• Check / replace pre- ilter
• Check air low monitor and re-calibrate i necessary
• Check sa ety interlocks and alarms
• Inspect electrical components, lighting, cables etc.
• Issue test report and air low certi icate.
For parts or service information please contact Monmouth Scientific on:
[phone]

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Monmouth Scientific
Units 5 & 6 Kilnside
East Quay
Bridgwater
Somerset TA6 4DB
Email: info monmouthscientific.co.uk
Monmouth Scientific is a trading division of Monmouth Surgical Ltd.