MVE CryoCube Instruction Manual

MVE CryoCubeTM Operating
Instruction Manual
MVE CryoCube is i eally suite for the transportation of biological
materials. The liqui nitrogen is retaine in an absorbent material
an the col nitrogen vapors maintain the CryoCube storage area at -
150°C. The absorbent material retains the liqui nitrogen an
prevents acci ental spills.
Liqui nitrogen is classifie as “Dangerous Goo s” by the
Department of Transportation (DOT). At least three regulatory
agencies publish angerous goo s regulations:
DOT - Department of Transportation
IATA - International Air Transportation Association
ICAO - International Civil Aviation Organization
Liqui nitrogen is classifie un er the name “Nitrogen, refrigerate
liqui ” an has a UN 1977 co e number. The name classification
an UN Co e Number must be in icate on the shipping carton in
a ition to a “Gas nonflammable” label. Packing an labeling
requirements can be obtaine from the above organizations. Also,
contact your carrier for any variances in the rules as they may apply
specifically to that company an /or the ultimate estination of the
shipment.
Be aware that the regulations un ergo perio ic revisions.
If you nee current information prior to the shipping of your
cryobiological specimen, please contact your air carrier for the
most recent regulations.
GENERAL DESCRIPTION:
The CryoCube is a vacuum insulate aluminum
container with a fiberglass neck construction provi ing
the highest efficiency possible in nitrogen vapor
storage. The container is for use with inert flui s only.
Liqui oxygen is not compatible with this unit an
must not be store .
These high quality vacuum insulate units are
constructe of urable material, compatible with the
ivergent temperature extremes an broa applications
of cryobiology. The life expectancy of this pro uct is
one (1) year.
Upon receipt of the container, examine both the
container an packaging for any evi ence of amage
uring shipping. Contact the shipping agent if any
amage is apparent. Watch after the first fill for any
signs of vacuum loss, such as frost or sweating on the
outsi e jacket. (Some frost near the top just after filling
is normal).
A) SAFETY
To avoi injury by frostbite, use extreme care whenever
han ling
liqui nitrogen, liqui nitrogen storage or transfer
vessels or any objects which have come in contact with
liqui nitrogen.
• Leave no area of skin expose .
• Always wear proper safety attire over clothing:
face shiel , cryogenic gloves, cryogenic apron.
• Never overfill vapor shippers with liqui nitrogen.
Overfilling the tank may cause imme iate or
premature vacuum failure to occur.
• Always keep vapor shippers in upright position.
• Do not tightly seal liqui nitrogen container or
prevent nitrogen gas from escaping.
• Use extreme care to prevent spilling an
splashing liqui nitrogen uring transfer.
• Imme iately remove any clothing or safety
attire on which liqui nitrogen has spille .
• Get imme iate me ical attention for any
frostbite injuries ue to liqui nitrogen.
WARNING: The venting of nitrogen vapors will eplete
oxygen in the air, possibly lea ing to asphyxiation or even
eath. Do not store or use container in areas that have
poor ventilation.
B) CHARGING / FILLING INSTRUCTIONS
Note: Fill the container with a funnel when possible. Avoi
spilling liqui nitrogen on the neck. To ensure maximum
performance from
your MVE CryoCube simply follow the liste
steps just prior
to shipping to final estination:
• Weight empty unit an recor . The unit shoul
weigh about 5.2 ± .2 lbs (2.4 ± .1kg)
• Using a funnel pour 1 cup (8oz, 250ml) of LN2
• Wait 5 minutes or until LN2is absorbe
• Continue a ing 1 cup of LN2every few minutes
until 3.5 lb (1.6 kg) have been a e . This shoul
be ~10 cups.
• Verify internal tank is ry, pour off any stan ing
liqui .
• Insert contents into canister an canister into
ewar.
• Insert cork half way into neck an allow to cool
for ~1 minute.
• Push cork all the way into neck.
• The container is now rea y for vali ation, labeling
an shipping.

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C) PREVENTIVE MAINTENANCE
The MVE CryoCube is esigne as a limite use pro uct an shoul
be consi ere a isposable shipper. Generally, no preventive
maintenance is require for the CryoCube. However, if the unit
performance rops below an acceptable hol time (< 5 ay hol ing
time @ >-150C), users shoul contact Chart Inc. Customer/Technical
Service for rework options. The rework options may exten the
overall life of the unit.
D) SHIPPING INSTRUCTIONS
Follow charging instructions. Prior to packaging ewar for shipment
the entire contents of liqui nitrogen must be remove . This is
usually one by pouring out excess liqui nitrogen until no liqui is
visible on the bottom of the inner ewar. Place in bio samples,
package ewar an ship. If liqui nitrogen is visible in the bottom of
the inner it then becomes a liqui shipper an the exception status is
voi . The liqui insi e is now classifie as hazar ous material.
LETTER EXPLAINING EXCEPTION
This is concerning the applicability of the Fe eral Hazar ous Material
Regulations to the shipment of refrigerate samples in the “Dry
Shipper” container. A “Dry Shipper” package consists of an outer
container that is line with an absorbent material. The container is
charge with nitrogen refrigerate liqui which is absorbe into the
container lining. The charge , complete package serves as
refrigerate container for the shipment of samples.
In consi eration of the above, consultation with the Research an
Special Programs A ministration of the DOT has etermine that the
use of nitrogen refrigerate liqui charge “ ry shipper” containers
for the shipment of samples fall within the regulation exception
provi e in 49CFR 173.320 paragraph (a) of the section states the
requirements of this subchapter o not apply to atmospheric gases
an helium when use in the operation of the process system such
as refrigeration system. Paragraph (c) of 173.320 pertains to air
transport of same refrigeration system. For exception status of air
shipments please refer to IATA-Dangerous Goo s Regulations for
nitrogen refrigerate liqui . This falls in the class of 2.2
nonflammable gas, packing instructions 202 with special provisions
A-800. For answers to questions regar ing shipping regulations
contact a Chart, AI-Cryobiological Tech Service Representative at
800-482-2473.
Chart BioMedical Group,
Chart, Inc.
2200 Airport In ustrial Drive, Suite 500
Ball Groun , GA 30107
Unite States
Chart BioMedical Products,
Unit 2 / Max ata Centre,Downmill Roa
Bracknell
Berkshire RG12 1QS
Unite King om
Chart Australia Pty Ltd.
Sydney Business & Technology Centre
Unit 43 / 2 Railway Para e
Li combe NSW 2141, Australia
Chart BioMedical Group,
Chart, Inc.
155 Don Westbrook Ave. S.
Jasper, GA 30143
Unite States
Ref 14456068 Rev A
TM

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LIMITED WARRANTY STATEMENT
MVE warrants to the original purchaser (“Purchaser”) that each new CryoCube (each a “Product”), shall be ree
rom de ects in materials and workmanship or a period o ninety (90) days rom the date o shipment, except as
provided below. MVE warrants all dewar vacuum integrity shall be ree rom de ects in materials and workmanship
or a period o ninety (90) days rom the date o shipment, except as provided below. MVE warrants all repaired
equipment and actory blemished equipment shall be ree o de ects in materials and workmanship or a period o
ninety (90) days rom the date o shipment and warrants all dewar vacuum integrity shall be ree rom de ects in
materials and workmanship or a period o ninety (90) days rom date o shipment.
Purchaser agrees that be ore this limited warranty shall become e ective, Purchaser shall ully inspect each Product
within ten (10) days o delivery and be ore such Product is put to use. Purchaser also agrees to operate the Product
in accordance with MVE’s operating instructions and that ailure to do so shall void this limited warranty.
Purchaser urther agrees that any claim or breach o warranty must be made in writing within 15 days o discovery
o a purported de ect. MVE will not be responsible or any alleged breach o warranty, which, as a result o MVE’s
inspection, MVE determines to have arisen rom a cause not covered by this limited warranty.
This limited warranty does not apply to: (A) Misuse, abuse, accident, or repairs made by persons other than MVE or
persons not authorized by MVE; (B) De ects caused by the e ects o normal wear and tear; (C) Acts o God, or other
causes not within the control o MVE.
I purchaser believes that a product does not comply with the limited warranty stated above, Purchaser should
contact MVE, describing the problem and providing proo o the date o purchase. I directed by MVE Purchaser
shall return Products reight prepaid, properly packaged in a MVE approved shipping container and properly
identi ied by a Return Material Authorization Number issued by MVE. Products returned without a Return
Material Authorization Number will be re used and returned at Purchaser’s expense.
MVE SHALL HAVE NO FURTHER LIABILITY FOR DAMAGES, LOSSES, COST OR FEES OF ANY KIND OR
NATURE, WHETHER FORESEEABLE OR NOT, INCLUDING BUT NOT LIMITED TO ATTORNEY’S FEES
AND CONSEQUENTIAL, GENERAL, SPECIAL, EXEMPLARY OR PUNITIVE DAMAGES, ARISING OUT
OF OR RELATED TO THE USE OF MVE EQUIPMENT, EVEN IF MVE HAS BEEN ADVISED OF THE
POSSIBILITY OF SUCH DAMAGES, LOSSES, COST OR FEES.
EXCEPT FOR THIS LIMITED WARRANTY, MVE HAS MADE NO WARRANTIES OR
REPRESENTATIONS, EXPRESSED, OR IMPLIED, INCLUDING BUT NOT LIMITED TO IMPLIED
WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. NO
REPRESENTATION OR STATEMENT OF MVE MAY CHANGE OR ALTER THIS LIMITED WARRANTY.
Any claims or breach o this limited warranty shall be governed by Georgia law and must be brought in a state or
ederal court in Georgia. Some states do not allow limitations on implied warranties or on incidental or
consequential damages, so the above limitations may not apply. This limited warranty gives the purchaser speci ic
legal rights. Purchaser may also have other rights, which vary rom state to state.