Sarstedt Sarpette M User manual
Instructions for Use
Sarpette®M
SARSTEDT No.: 90.3100.xxx / 90.3108.xxx / 90.3112.xxx
2 3
Instructions for Use Sarpette® MInstructions for Use Sarpette® M
Contents
1. Product description 3
1.1 Safety instructions 3
1.2 Description 3
2 Commissioning 3
2.1 Positioning for work with the pipette 3
2.2 Set volume 3
2.3 Tip selection 4
2.4 Tip ejection 4
2.5 Protectivelter 4
3 Pipetting procedure 4
3.1 Normal pipetting procedure 5
3.2 Reverse pipetting procedure 5
4 Maintenance 5
4.1 Checking the seal 5
4.2 Cleaning 6
4.3 Disassembly of the volume unit and replacement of the O-ring, models – 1000 µl 6
4.3.1 Remove the volume unit (lower part) 6
4.3.2 Replacing the O-ring and PTFE sleeve 6
4.3.3 Replacing the O-ring 6
4.4 Disassembly of the volume unit and replacement of the O-ring, models 5 and 10 ml 7
4.4.1 Remove the volume unit (lower part) 7
4.4.2 Changing the O-ring 7
4.5 Piston change, multi-channel Sarpette®M 8
5 Faults 8
6 Sterilisation 8
7 Calibration 9
7.1 In-lab calibration 9
8 Warranty 9
9 Performance data Sarpette® M 10
9.1 Single-channel Sarpette®M 10
9.2 Multi-channel Sarpette®M8 / M12 10
10 Ordering information 11
1. Product description
1.1 Safety instructions
• Read instruction manual carefully before use and keep for future reference.
• Follow the manufacturer's instructions and instructions for use and maintenance.
• Avoid possible overexertion of the hand during prolonged pipetting work and its medical consequences
(e.g. tendonitis).
1.2 Description
The Sarpette®Mallowspreciseandeortlesspipetting.Thesingle-channelmodelscoveravolumerangeof
0.1μlto10ml.Thesixmodelsofthe8-or12-channelversionsintherangeof0.5to300μlareperfectlysuited
for a uniform liquid transfer in microtiter plates. All models are fully assembled and autoclavable at 121°C. The
main advantages are the digital display (E) for a permanent volume display and the innovative JustipTM system
(H) with a softly padded ejection button (C) for optimum tip ejection. The Swift Set calibration system (F) allows
readjustments by the user.
2 Commissioning
I
H
G
F
E
D
C
B
A
I
A
B
C
D
E
F
H
K
~ 0.5 mm
~ 0.5 mm
~ 0.5 mm
~ 0.5 mm
~ 0.5 mm
2.1 Positioning for work with the pipette
Placethengerrest(D)onthedistalphalangeoftheindexnger.Thepushbutton(B)andtipejector(C)can
be actuated by a slight movement of the thumb. The rotatable multi-channel housing (K) enables individual
selection of the best possible adapted working position.
2.2 Set volume
Thevolumeisadjustedbyturningthepushbutton(B)untilthedesiredvolumeappearsinthedigitaldisplay(E)
(turning clockwise reduces the volume and vice versa). Sensitive click stops of the micrometre screw and the
freely rotating coloured pushbutton cap (A) prevent unwanted adjustments during ongoing pipetting operations.
Fig. 1 Fig. 2 Fig. 3
4 5
Instructions for Use Sarpette® MInstructions for Use Sarpette® M
When the letter (E) lights up next to the numbers in the display, the selected volume is
no longer in the working range of the pipette. Over-rotating the micrometre screw can
damage the mechanism.
2.3 Tip selection
TheSARSTEDTRellRevolutionpipettetipsprovidethebestpossiblecombinationofpipetteandtipforevery
application.Useonlytipsoeredbythemanufacturerorcompatiblewiththem.Furtherinformationanda
detailedbrochurecanbefoundatwww.rellrevolution.tips.
Reproducibilityisimprovedifeachtipisushedwiththematerialtobepipetted
at least once before use.
2.4 Tip ejection (Figs. 2 and 3)
The patented JustipTM system allows immediate positioning of the shaft height (± 2 mm) for an ideal adjustment
and perfect ejection of the tips. The distance between the tip and the ejection rod is set to ~0.5 mm by turning
theejectionscrew(<LO–HI>).Built-inclickstopspreventunwantedadjustments.
2.5 Protectivelter
Macropipettes(5and10mlmodels)canbeprovidedwithaprotectivelterforadditionalsafetyagainst
penetration of liquid and risk of contamination. This is recommended when dosing large volumes and/or toxic,
radioactiveorhighlyaggressiveliquids.Itisessentialtoreplaceawetorcontaminatedlter.Theltersarenot
autoclavable.
3 Pipetting procedure
Beforepipetting,anew,cleantipmustbermlyattached.
~ 0.5 mm
1 2 3 4c
1 2 3 4c
3.1 Normal pipetting procedure (Fig. 4)
The precisely adjusted volume is aspirated and then discharged.
- Phase1: Presstheoperatingbuttonuntiltherststopisreached.
- Phase 2: Immerse the tip vertically approx. 2 – 3 mm deep and slowly release the button. Wait 2
seconds,removepipetteandlledtipverticallywithouttouchingthecontainer.
- Phase 3: Place the tip against the wall of the second container, slowly press the operating button to
therststoptoejectliquid.
- Phase 4: Press the operating button completely. Residues are expelled from the tip.
Draw the pipette tip 10-15 mm along the wall of the receiver tube.
- Phase 5: Discard the used tip by pressing the ejection button (C).
3.2 Reverse pipetting procedure
Any excess volume is aspirated, but only the set volume is dispensed.
- Phase 1: Press the operating button until the overstroke (second stop) is reached.
- Phase 2: Immerse the tip vertically approx. 2-3 mm deep and slowly release the button. Wait 2 sec.,
removethepipetteandlledtipverticallywithouttouchingthecontainerwall.
- Phase3: Placethetiponthewallofthesecondcontainerandpressbuttononlyuptotherst
stop, to deliver the exact set amount of liquid.
- Phase 4: Remove the pipette from the second container and repeat Phase 2. Expel the remaining
content by pressing the button until the second stop (overstroke). Discard the used tip by
pressing the ejection button (C).
4 Maintenance
As a rule, the models do not require maintenance. Continuous care and cleaning contribute to the optimal
function and longevity of the instrument. It is recommended to check the performance data according to internal
control procedures (SOP, GLP, etc.) or at least once a year. Replace defective parts only with original parts from
the manufacturer.
3
2
1
2
1
3
3
4
flexion sur la tête du piston.
4.1 Checking the seal
Correct functioning of the instrument is only possible if the air cushion closes tightly. Leakage is shown by
dropletformationatthetiporbyadierentliquidlevelintheindividualtipsofamultichannelpipette.Intheevent
of a leak, we recommend that you have the pipette serviced.
Reverse pipetting Fig. 5Forward pipetting Fig. 4
Fig. 6 Fig. 7 Fig. 8
6 7
Instructions for Use Sarpette® MInstructions for Use Sarpette® M
4.2 Cleaning
The removed volume unit (lower part) of the pipette can be cleaned with water or alcohol. In cases of heavy
soiling, the individual parts can also be immersed in a disinfectant solution. Proper cleaning is necessary if liquid
has inadvertently penetrated into the pipette interior. Rinse and dry the instrument before assembly. Remove
particularlystubbornresiduesintheultrasonicbath.Beforeassemblingthepipette,lightlygreasetheO-ring(see
below).
4.3 Disassembly of the volume unit and replacement of the O-ring, models – 1000 µl (Fig. 6)
4.3.1 Remove the volume unit (lower part):
1. Press the ejection button (2), turn the ejection nut (20) or (24) to the left for 2 ml and remove.
2. Unscrew the cylinder (18).
3. Push the operating button down completely, then pull out the piston.
4.3.2 Replacing the O-ring and PTFE sleeve:
1. After removing the lower part, remove the cylinder (18) and press it onto both mandrels of the ring (14) with
the aid of a pipette tip or a pointed object.
2. Release the cylinder ring, pull out the spring (15) to reach the O-ring/PTFE sleeve.
3. Replace defective parts. Remove excess grease from the piston (13).
To avoid possible damage to the PTFE sleeve, place it on
the piston (13) and only then attach the O-ring (17).
4. Lightly grease the piston, PTFE sleeve and O-ring and reassemble the volume unit.
TheO-ringSealCuAssemblyisnotaccessibleon2,10,10Yand20μlmodels.Ifthereisa
lack of tightness, the complete cylinder must be replaced.
4.3.3 Replacing the O-ring:
1. Unscrew the piston rod (21), remove the spring positioners (22, 23) and the spring (15).
2. If necessary, replace defective parts. Grease the O-ring (17) and cylinder (18) evenly.
4.4 Disassembly of the volume unit and replacement of the O-ring, models 5 and 10 ml (Fig. 7)
1
2
34
5
6
7
8
9
10
11
33
12
13
18
19
20
12
14
15
16
17
22
21
15
23
17
13
24
25
18
26
20
815 : 1, 2, 5, 10 L
825 : 2, 10 L
(except 825.0010Y)
815/825 : all other models 835 : all models
12
13
18
19
20
12
13
18
19
20
12
14
15
16
17
22
21
15
23
17
13
24
25
18
26
20
815 : 1, 2, 5, 10 L
825 : 2, 10 L
(except 825.0010Y)
815/825 : all other models 835 : all models
12
13
18
19
20
815 : 1, 2, 5, 10 L
825 : 2, 10 L
(except 825.0010Y)
12
13
18
19
20
815/825:
all other sizes
815:
1,2, 5, 10, 20 µL
825:
2, 10, 10Y, 20 µL
All models
12
22
21
15
23
17
13
24
25
18
26
20
835 : all models
27
28
29
30
32
31
855: all sizes835: 5 and 10 mL
1. Ejector cushion 12. Circlip 23.Bottomspringpositioner
2. Ejection button 13. Piston 24 Ejection cap
3. Spring washer 14. Ring 25. Ejection sleeve
4. Smartie Cap 15. Spring 26. Ejection screw
5. Ejection spring 16. PTFE sleeve 27. Cover assembly
6. Counter unit (adjustable) 17. O-Ring (piston) 28. Arm
7.Dosingunit(x) 18. Cylinder 29. Piston assembly
8. Handpiece 19. Ejector 30. Cylinder unit
9. Window 20 Ejection nut 31. Housing
10 Screws, handpiece (2x) 21. Piston rod 32. O-ring (cone, 200 µl)
11. Ejection rod 22. Top spring positioner 33. Calibration slide
4.4.1 Remove the volume unit (lower part):
1. Turn the ejection nut (20) and separate it from the ejection cap (24).
2. Unscrew the cylinder (18), press the operating button, then pull out the piston.
4.4.2 Changing the O-ring:
1. Press down both clips of the cylinder (18) simultaneously and without exerting force and separate them from
the ejection sleeve (25). Remove piston assembly.
2. Unscrew the piston rod (21), remove the spring positioners (22, 23) and the spring (15).
3. If necessary, replace defective parts. Grease the O-ring (17) and cylinder (18) evenly.
4. Reassemble the piston assembly, cylinder and ejection sleeve.
5.Screwthecylinderontothepipette,presstheoperatingbutton(B)downfullyandlockthepistonrod.
8 9
Instructions for Use Sarpette® MInstructions for Use Sarpette® M
4.5 Piston change, multi-channel Sarpette®M
A piston change in multi-channel pipettes, as well as the replacement of other defective parts, should always be
carried out by their local service partner.
5 Faults
Problem Possible causes Solution
Tipisnotxed
on cone
Tip ejector incorrectly positioned
Unsuitable tips
Adjust the position of the tip ejector
Use original or compatible tips
Piston sticks,
moves irregularly
Soiled piston Disassemble and clean piston
(also grease 5 and 10 ml pipettes and
multi-channel pipettes)
Liquid is not
aspirated
Blockedcone
Volumetric unit of pipette incorrectly
assembled
Contaminatedprotectivelterfor5and
10 ml pipettes
Dismantling and cleaning
Assemble the lower part according to
the instruction manual
Replaceprotectivelter
Dierent
liquid level
in tips of
multi-channel pipettes
Lack of tightness Checkthetofthepipettetips
Replace defective cone, PTFE sleeve or
O-ring
Instrument does not
perform as required
Lack of tightness
Unsuitable tips
Contaminatedprotectivelterfor5and
10 ml pipettes
Instrument calibrated incorrectly
Use of the instrument with viscous,
volatile or extremely temperature-
controlled liquids
Check whether cone, PTFE sleeve or
O-ring is defective
Checkcompatibilityandtofpipette
tips
Replaceprotectivelter
Perform recalibration
Re-calibrate with appropriate liquid
6 Sterilisation
The design of the Sarpette®M takes into account repeated sterilisation in an autoclave at 121 °C. (20 minutes).
Formodels5and10ml,removetheprotectivelterbeforeautoclaving.Placetheinstrumenthorizontallyinthe
autoclave,avoiddirectcontactwithmetal.Beforeuse,checkthatthepipetteisdryandcompletelycooled.For
model5and10ml,installnewprotectivelter.Checkthesealingandperformancedataregularly,butatleast
after 50 autoclaving cycles. Re-tighten the volume unit if loose. A change in the colour of the material as well
asmoredicultrotationofthecolouredcapmayoccurafterrepeatedautoclaving.Correctautoclavingand
resulting sterility are the responsibility of the user.
7 Calibration
Each Sarpette®Mwascalibratedafterproductionandindividuallytested.Thecontrolcerticatesuppliedwith
the pipette contains all data as well as its serial number. The performance data are checked with distilled water
at a constant (± 0.5 °C) room temperature between 20 and 25 °C, in accordance with ISO 8655.
7.1 In-lab calibration
With the Swift-Set calibration system, the pipettes can be quickly and safely adjusted to the correct volume as
follows:
• Remove the coloured cap (a) from the push button and remove the calibration screw (b).
•Pullothecalibrationseallabel(c)andsetthecalibrationslide(d)tothepositioncalusingapipettetipor
pointed object.
• Turn the calibration screw until the average measured volume appears on the digital display without
holding the push button.
• Move the calibration slide back into the lock position, place the coloured cap on the push button.
• After a few piston movements, check the result.
• Stick the recalibration seal label to the calibration slide.
8 Warranty
The Sarpette®M is guaranteed against any manufacturing and material defects for the period of time mentioned
intheinspectioncerticate.Damageresultingfromdisregardoftheoperatingandsafetyinstructionsor
autoclaving at improper temperature as well as colour deviations of the materials are excluded from the warranty.
Repairs and replacement of spare parts do not extend the warranty period. If there are malfunctions that cannot
berectiedaccordingtotheaboveinstructions,contactyourSARSTEDTcontactperson.
Fig. 12 Fig. 13
Instructions for Use Sarpette® M
11
Instructions for Use Sarpette® M
9 Performance data Sarpette® M
9.1 Single-channel Sarpette®M
9.2 Multi-channel Sarpette®M8 / M12
The performance values were determined with distilled water at a constant temperature (± 0.5 °C) between 20 and 25 °C in accordance with
ISO 8655.
1) measured at 10% nominal volume
Volume
Order no. Volume
range Increment
Number
of
channels
90.3108.010 0.5 – 10µl 0.01 µl 8
90.3108.200 20 – 200 µl 0.2 µl 8
90.3108.300 30 – 300 µl 0.4 µl 8
90.3112.010 0.5 – 10µl 0.01 µl 12
90.3112.200 20 – 200 µl 0.2 µl 12
90.3112.300 30 – 300 µl 0.4 µl 12
Imprecision (E %)
Min. vol. Intermediate
vol. Max. vol.
<+/– 3.5 %1) <+/– 2.5 % <+/– 1.5 %
<+/– 0.9 %1) <+/– 0.8 % <+/– 0.7 %
<+/– 1.0 %1) <+/– 0.9 % <+/– 0.8 %
<+/– 3.5 %1) <+/– 2.5 % <+/– 1.5 %
<+/– 0.9 %1) <+/– 0.8 % <+/– 0.7 %
<+/– 1.0 %1) <+/– 0.9 % <+/– 0.8 %
Imprecision (CV %)
Min.
vol.
Intermediate
vol.
Max.
vol.
< 3.0% < 2.0% < 1.0%
< 0.6% < 0.5% < 0.3%
< 0.6% < 0.5% < 0.3%
< 3.0% < 2.0% < 1.0%
< 0.6% < 0.5% < 0.3%
< 0.6% < 0.5% < 0.3%
Order no. Description Volume
range
Inner
Packaging
Suitable SARSTEDT
pipette tips
90.3100.002
Single-channel pipettes
0.1 – 2 µl
1 pc./box
70.3010.xxx
70.3020.xxx
70.3021.xxx
90.3100.010 0.5 – 10 µl
70.3010.xxx
70.3020.xxx
70.3021.xxx
90.3100.020 2–20 µl 70.3030.xxx
70.3031.xxx
90.3100.100 10 – 100 µl 70.3030.xxx
70.3031.xxx
90.3100.200 20 – 200 µl 70.3030.xxx
70.3031.xxx
90.3100.000 100 – 1000 µl 70.3050.xxx
70.3060.xxx
90.3100.555 0.5 – 5 ml 70.1183.102
70.1183.002
90.3100.111 1 – 10 ml 70.1187.102
70.1187.002
90.3108.010
8-channel pipettes
0.5 – 10 µl
1 pc./box
70.3010.xxx
70.3020.xxx
70.3021.xxx
90.3108.200 20 – 200 µl 70.3030.xxx
70.3031.xxx
90.3108.300 30 – 300 µl
70.3030.xxx
70.3031.xxx
70.3040.xxx
90.3112.010
12-channel pipettes
0.5 – 10 µl
1 pc./box
70.3010.xxx
70.3020.xxx
70.3021.xxx
90.3112.200 20 – 200 µl 70.3030.xxx
70.3031.xxx
90.3112.300 30 – 300 µl
70.3030.xxx
70.3031.xxx
70.3040.xxx
BA051-0722 Technicalmodicationsreserved p/n03-0-0002-0197
SARSTEDT AG & Co. KG
P.O.Box1220·D-51582Nümbrecht
Phone: +49 2293 305 0
Fax: +49 2293 305 3450
Customer Service Germany
Phone 0800 0 83 305 0
www.sarstedt.com
Volume
Order no. Volume
range Increment
90.3100.002 0.1 – 2 µl 0.002 µl
90.3100.010 0.5 – 10µl 0.01 µl
90.3100.020 2 – 20 µl 0.02 µl
90.3100.100 10 – 100 µl 0.01 µl
90.3100.200 20 – 200 µl 0.2 µl
90.3100.000
100 – 1000 µ
l1 µl
90.3100.555 0.5 – 5ml 0.01 ml
90.3100.111 1 – 10 ml 0.01 ml
Imprecision (E %)
Min. vol. Intermediate
vol. Max. vol.
<+/– 6.0 % <+/– 4.0 % <+/– 2.0 %
<+/– 2.5 % <+/– 1.8 % <+/– 1.0 %
<+/– 2.5 % <+/– 1.8 % <+/– 1.0 %
<+/– 1.5 % <+/– 1.2 % <+/– 0.8 %
<+/– 1.5 % <+/– 1.1 % <+/– 0.6 %
<+/– 1.5 % <+/– 1.0 % <+/– 0.5 %
<+/– 1.5 % <+/– 1.1 % <+/– 0.6 %
<+/– 1.5 % <+/– 0.7 % <+/– 0.5 %
Imprecision (CV %)
Min. vol. Intermediate
vol. Max. vol.
< 5.0% < 3.3% < 1.5%
< 1.8% < 1.2% < 0.5%
< 1.7% < 1.0% < 0.5%
< 1.0% < 0.6% < 0.2%
< 0.6% < 0.4% < 0.2%
< 0.5% < 0.4% < 0.2%
< 0.6% < 0.5% < 0.3%
< 0.5% < 0.3% < 0.2%
10 Ordering information
10
This manual suits for next models
9
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