Myron L ULTRAPENX2 PTBT5 User manual

PTBT5OM-X2 01-20
Operation
Manual
ULTRAPENX2™ PTBT5
Dissolved Oxygen & Temperature
Pocket Tester
For use with PTBTX2™ App
and Your iOS™ or Android™ Mobile Device

ULTRAPENX2™ PTBT5 Operation Manual
Dissolved Oxygen (DO) and Temperature Pen for use with your Mobile Device Running the PTBTX2™ App
PTBT5OM-X2 01-20
Table of Contents
INTRODUCTION ............................................................................................................ 1!
FEATURES ................................................................................................................... 1!
I.!PTBT5 - Layout ........................................................................................................ 1
II!PTBTX2 App Measurement Screen.......................................................................... 1!
III.!Feature Navigation Bar ............................................................................................ 1!
PREPARING THE DO SENSOR .................................................................................... 2!
USING THE PTBT5 ....................................................................................................... 2!
Step 1 – Download the “Ultrapen PTBTX2” App ............................................................. 2!
Step 2 – Connect the PTBT5 to the App ......................................................................... 2!
Step 3 – Choose a Measurement Parameter Setting ...................................................... 3!
Step 4 – Choose the Ultrapen Mode Setting ................................................................... 4!
Step 5 – Set the Location Altitude ................................................................................... 5!
DISSOLVED OXYGEN MEASUREMENT ...................................................................... 6!
I.!Measurement Setup ................................................................................................. 6!
II.!PTBT5 HOLD Mode Measurements ......................................................................... 6!
III.!PTBT5 LIVE Mode Measurements ........................................................................... 7!
CALIBRATION .............................................................................................................. 8!
I.!General Notes: ......................................................................................................... 8!
II.!Sensor Check .......................................................................................................... 8!
III.!Zero Calibration ....................................................................................................... 9!
IV.!Air Calibration (Water-Saturated Air) .......................................................................10!
V.!Water Calibration (Air-Saturated Water) ..................................................................11!
VI.!Temperature Controlled, Full Calibration Procedure ................................................12!
VII.!Factory Calibration Reset ........................................................................................15!
EXPORTING DATA FILES ...........................................................................................16!
MAINTENANCE............................................................................................................17!
I.!Routine Maintenance ..............................................................................................17!
II.!Storage ...................................................................................................................17!
III.!Replacing / Refilling the DO Sensor Electrolyte Solution ..........................................18!
IV.!DO Membrane Replacement ...................................................................................18!
V.!Cleaning the DO Sensor .........................................................................................19!
VI.!Replacing or Reinstalling the DO Sensor .................................................................20!
VII.!Battery Replacement...............................................................................................21!
TROUBLESHOOTING GUIDE ......................................................................................22!
SPECIFICATIONS ........................................................................................................23!
ACCESSORIES ............................................................................................................23!

ULTRAPENX2™ PTBT5 Operation Manual
Dissolved Oxygen (DO) and Temperature Pen for use with your Mobile Device Running the PTBTX2™ App
PTBT5OM-X2 01-20 1
INTRODUCTION
Thank you for purchasing the Myron L® Company ULTRAPENX2 ™ PTBT5 Dissolved Oxygen (DO) Pen. This instrument
is designed to be extremely accurate, fast, and simple to use in diverse water quality applications. Advanced features
include:
• Real time readings.
• Proprietary sensor design and microprocessor-based circuitry that measures DO with an accuracy of up to ± 2%.
• Temperature compensation algorithm that dynamically corrects for changes in membrane / sample temperature.
• Adjustment for Altitude and sample Salinity.
• Three calibration methods, AIR (Water Saturated Air), WATER (Air Saturated Water), and ZERO (0 ppm DO).
• A rugged, reliable design with an aircraft aluminum body.
• Waterproof housing.
• Replacement membranes contained in easy to install, screw-on caps.
• Enabled.
FEATURES
I. PTBT5 - LAYOUT
1. PEN BUTTON – Press to turn PTBT5 ON and place it in Standby mode.
2. BATTERY CAP – Unscrew to change battery.
3. LED INDICATOR LIGHT – Flashes rapidly when the PTBT5 is measuring and
once every 5 seconds when the PTBT5 is in Standby mode.
4. DO SENSOR BODY – Contains electrodes and reagent for
Dissolved Oxygen and Temperature measurements.
5. DO SENSOR CAP – Holds the DO sensor membrane
in place.
6. DO MEMBRANE – Permeable membrane through
which Dissolved Oxygen passes.
7. PROTECTIVE / HYDRATION CAP – Protects sensor from damage. The sensor should be kept moist at all times.
• Always replace the protective / hydration cap containing a sponge moistened with DI, RO or distilled water.
• See the MAINTENANCE section below for instructions for storing > 1 month.
• When removing or replacing the cap, gently wiggle it back and forth while pulling / pushing. DO NOT twist!
• Vent Hole: Allows air pressure to equalize during AIR calibration.
8. CAP STOP – Pushing the protective / hydration cap beyond the cap stop could damage the sensor.
II PTBTX2 APP MEASUREMENT SCREEN
1. MEASUREMENT VALUE FIELD – Displays the measured value of the solution.
When the PTBT5 turns OFF, displays the message, "Ultrapen is Offline".
2. UNITS OF MEASURE – Displays DO units of measure (ppm or %).
3. SECONDARY MESSAGE FIELD – In Default mode displays DO Saturation.
Otherwise displays the current parameter (DO Concentration or Saturation)
4. SOLUTION TEMPERATURE – Measured temperature of the solution.
5. PTBT5 BATTERY LEVEL – Flashes RED when PTBT5 battery is ≤ 25%.
6. MEMORY STORE BUTTON – Tap here to record the measurement in the App's
database.
7. MEMORY REPLACE BUTTON – Tap here to replace a previously stored
measurement with data from a new measurement.
8. MEASURE BUTTON – Tap this to start a new measurement.
9. FEATURE NAVIGATION BAR – These buttons activate various App features.
III. FEATURE NAVIGATION BAR
This bar appears at the bottom of all screens. It’s used to navigate between the App’s main functional areas.
MEASUREMENT – Returns the App to the Measurement screen.
MEMORY RECALL – Displays a list of saved measurements.
CALIBRATION – Places the App in Calibration mode.
CONNECT – Opens the App's Bluetooth Connect / Disconnect screen. Once a PTBT5 is connected to the App the button label changes
to say, “Disconnect."
PREFERENCES – Opens the App's Preferences & Settings modes, including an internet link to the full App Operation Manual (HELP).

ULTRAPENX2™ PTBT5 Operation Manual
Dissolved Oxygen (DO) and Temperature Pen for use with your Mobile Device Running the PTBTX2™ App
PTBT5OM-X2 01-20 2
PREPARING THE DO SENSOR
1. Unscrew the membrane cap from the sensor body by turning in a
counter-clockwise direction.
2. Attach the tip onto the syringe and fill the syringe with
DO electrolyte solution.
3. Rinse the inside of the membrane cap with
electrolyte solution.
4. Pour some electrolyte inside the membrane cap and set aside.
5. Hold the PTBT5 vertically with the DO sensor at the top and the
sensor opening facing up.
6. Insert the syringe tip into one of the four openings of the sensor body as far as it can go.
7. Slowly fill the electrolyte well with electrolyte.
8. Hold the electrolyte filled sensor body in one hand and bring the electrolyte filled membrane cap closer with your other
hand.
9. Tilt the membrane cap and place it onto the sensor body.
10. Carefully start turning the membrane cap clockwise until it tightens firmly to the sensor body. Some electrolyte may
escape.
11. Rinse any residual electrolyte from the outside of the sensor body with clean water (DI, RO or distilled).
12 Fill the protective / hydration cap with enough clean water to soak the sponge inside (preferably DI, RO, or distilled
water).
13. Place the protective / hydration cap onto the DO sensor all the way to the cap stop.
14. Before using the PTBT5, let the new sensor stabilize for 2-4 hours with the protective / hydration cap in place.
USING THE PTBT5
STEP 1 – DOWNLOAD THE “ULTRAPEN PTBTX2” APP
On and look for
STEP 2 – CONNECT THE PTBT5 TO THE APP
1. If the PTBT5 is OFF, press and release the PEN BUTTON to turn the PTBT5 ON and place it in Standby mode.
2. On your Device’s Home screen, tap the blue PTBTX2 icon to open the PTBTX2 App.
3. Tap the CONNECT button at the bottom of the App display.
• The App will switch to the CONNECT screen.
4. Swipe down on the screen. The
PTBT5 will appear on the list.
5. Tap the PTBT5’s name when it appears.
Default: MLC-PTBTX[Hexadecimal ID]
6. The PTBT5 will move to the top of the
list and a check mark will appear.
NOTES:
• Some Mobile Devices switch their wireless transmitters to a low power mode when their battery level gets too low.
• This could affect how easily your PTBT5 and your device connect and communicate.
• ALWAYS keep your Mobile Device charge level as high as possible. If it is <40%, plug your Mobile Device into a charger.
SYSTEM REQUIREMENTS
iOS device running iOS 10.0 or later.
If you are using an iPad,
Search for iPhone Apps.
Android device running OS 7.0 or later.

ULTRAPENX2™ PTBT5 Operation Manual
Dissolved Oxygen (DO) and Temperature Pen for use with your Mobile Device Running the PTBTX2™ App
PTBT5OM-X2 01-20 3
STEP 3 – CHOOSE A MEASUREMENT PARAMETER SETTING
The PTBT5 allows you to select one of three different ways to display DO measurements:
• DEFAULT MODE: Displays DO Concentration (ppm) and DO Saturation (%) simultaneously.
• DO CONCENTRATION: Displays DO Concentration (ppm) only.
• DO SATURATION: Displays DO Saturation (%) only.
To choose which DO Parameter to display during measurements:
1. If the PTBT5 is OFF, press and release the PEN BUTTON to turn the PTBT5 ON and place it in Standby mode.
2. Tap the PREFERENCES button in the Feature Navigation Bar.
3. Tap the line that says Ultrapen Settings.
4. Tap the Parameter Select line.
5. The App will display the current parameter
setting.
6. Tap the Parameter field.
7. Select the measurement parameter.
8. Tap APPLY. The App will update the PTBT5
parameter.

ULTRAPENX2™ PTBT5 Operation Manual
Dissolved Oxygen (DO) and Temperature Pen for use with your Mobile Device Running the PTBTX2™ App
PTBT5OM-X2 01-20 4
STEP 4 – CHOOSE THE ULTRAPEN MODE SETTING
The PTBT5 has two Ultrapen measurement modes:
• HOLD mode (Default) – The PTBT5 displays measurements in real time until the measurement has stabilized, then the App
displays the final reading and puts the PTBT5 into Standby mode.
• LIVE mode – The PTBT5 sends a series of real-time readings that are displayed continuously for up to 5 minutes after
which the final reading is held on the App’s display and the App places the PTBT5 into Standby mode. The user may end
the LIVE mode measurement series at any time prior to that by tapping the BLUE button on the App display.
To select one of these modes:
1. If the PTBT5 is OFF, press and release the PEN BUTTON to turn the PTBT5 ON and place it in Standby mode.
2. Tap the PREFERENCES button in the Feature Navigation Bar.
3. Tap the line that says Ultrapen
Settings.
4. Tap the Pen Mode line on the screen.
5. The App will display the current mode
setting.
6. Tap the Pen Mode field.
7. Select Live or Hold, then tap APPLY.
8. Return to the Measurement screen and
tap the GREEN Measure Button.
9. Either tap the BLUE button to manually
stop LIVE mode, or it will stop by itself
after 5 minutes.

ULTRAPENX2™ PTBT5 Operation Manual
Dissolved Oxygen (DO) and Temperature Pen for use with your Mobile Device Running the PTBTX2™ App
PTBT5OM-X2 01-20 5
STEP 5 – SET THE LOCATION ALTITUDE
Altitude significantly effects the amount of oxygen that can dissolve in water. As altitude increases, the amount of
dissolved oxygen decreases. BEFORE making any DO measurements it is important to adjust the Altitude setting of the
PTBT5. Altitude can be adjusted in 100 ft. increments.
1. If the PTBT5 is OFF, press and release the PEN BUTTON to turn the PTBT5 ON and place it in Standby mode.
2. Tap the PREFERENCES button in the Feature Navigation Bar.
3. Tap the Altitude line.
4. When the Altitude Adjust
screen appears, tap the
Altitude field.
5. Slide the two selection
wheels up or down to set
the altitude to the nearest
100 ft.
6. Tap Apply.
7. Tap “Settings” in the upper
left to return to the main
settings screen.
STEP 6 – SET THE SAMPLE SALINITY
Salinity also effects the amount of oxygen that can dissolve in water. As salinity increases, the amount of dissolved oxygen
decreases. BEFORE making any DO measurements it is important to adjust the Salinity setting of the PTBT5. Salinity can
be adjusted in 1 ppt increments.
1. If the PTBT5 is OFF, press and release the PEN BUTTON to turn the PTBT5 ON and place it in Standby mode.
2. Tap the PREFERENCES button in the Feature Navigation Bar.
3. Tap the Salinity line.
4. When the Salinity Adjust
screen appears, tap the
Salinity field.
5. Slide the selection wheel up
or down to set the Salinity
to the nearest 1 ppt.
6. Tap Apply.
7. Tap “Settings” in the upper
left to return to the main
settings screen.

ULTRAPENX2™ PTBT5 Operation Manual
Dissolved Oxygen (DO) and Temperature Pen for use with your Mobile Device Running the PTBTX2™ App
PTBT5OM-X2 01-20 6
DISSOLVED OXYGEN MEASUREMENT
I. MEASUREMENT SETUP
ALWAYS :
1. Makes sure that the DO sensor is filled with electrolyte and allowed to stabilize.
2. Adjust the PTBT5 Altitude to match that of the measurement location.
3. Adjust the PTBT5 Salinity to match that of the sample solution.
4. Perform at least an AIR (water-saturated air) calibration of the PTBT5.
• For best results, perform a Temperature Controlled, Full Calibration (see the CALIBRATION sections below).
• If the expected measurement is < 3 ppm, perform a ZERO calibration (see the CALIBRATION sections below).
II. PTBT5 HOLD MODE MEASUREMENTS
1. If the PTBT5 is OFF, press and release the PEN BUTTON to turn the PTBT5 ON and place it in Standby mode.
2. COMPLETELY submerge the PTBT5 sensor in the sample solution.
3. Allow the PTBT5 to equilibrate by submerging the sensor in the sample solution for 1 minute prior to making a
measurement.
4. Tap the MEASUREMENT button in the Feature Navigation Bar.
5. Tap the GREEN Measure button.
6. Swirl the pen in the sample while the
PTBT5 is measuring. The PTBT5’s
LED will flash rapidly.
• The Measure button will be
inactive.
7. When the PTBT5 completes the
measurement the App will display the
values.
• The Measure button will turn
GREEN and be active.
• MS Button
Tap to record the last measurement.
• MS Replace Button
Tap to replace data in an existing record with data from the last measurement.

ULTRAPENX2™ PTBT5 Operation Manual
Dissolved Oxygen (DO) and Temperature Pen for use with your Mobile Device Running the PTBTX2™ App
PTBT5OM-X2 01-20 7
III. PTBT5 LIVE MODE MEASUREMENTS
1. If the PTBT5 is OFF, press and release the PEN BUTTON to turn the PTBT5 ON and place it in Standby mode.
2. COMPLETELY submerge the PTBT5 sensor in the sample solution.
3. Allow the PTBT5 to equilibrate by submerging the sensor in the sample solution for 1 minute prior to making a
measurement.
4. Tap the MEASUREMENT button in the Feature Navigation Bar.
5. Tap the GREEN Measure button.
6. Swirl the pen in the sample while the
PTBT5 is measuring. The PTBT5’s LED
will flash rapidly.
7. When the PTBT5 stabilizes, it will begin
displaying measurements.
a. A flashing, BLUE, LIVE mode indictor will
appear in the upper right corner of the
Measurement field.
b. A BLUE button will appear that allows the user
to manually end LIVE mode measurements at
any time.
8. Continue swirling the Ultrapen in the
sample solution while the LIVE mode
measurement series is continuing.
9. After 5 minutes, or if the user taps the
BLUE “End Live Mode” button, the
Ultrapen will send its last measurement
result and put itself in Standby mode.
10.The App will display the final
measurement values.
MS Button: During LIVE mode, tapping the MS
button will allow you to store each new
measurement. Once you save the
measurement, the App will automatically return
to the LIVE mode Measurement screen.

ULTRAPENX2™ PTBT5 Operation Manual
Dissolved Oxygen (DO) and Temperature Pen for use with your Mobile Device Running the PTBTX2™ App
PTBT5OM-X2 01-20 8
CALIBRATION
I. GENERAL NOTES:
• It is recommended that the PTBT5 be calibrated before each series of tests.
• Verify calibration if readings are not as expected. If the expected measurement is < 3 ppm, ZERO point calibration is
particularly important.
• For greatest accuracy, see the section titled: Temperature Controlled, Full Calibration.
Accuracy After Calibration:
• ZERO Calibration + AIR Calibration @ 25°C:
o Saturation:
! 0-100%: ± 45 counts; >100%: ± 4.5% of the reading (>5°C to ≤ 55°C).
o Concentration:
! ± 0.45 ppm or ± 4.5 % of the reading, whichever is greater (up to 40˚C).
• ZERO Calibration + WATER Calibration @ 25°C:
o Saturation:
! 0-100%: ± 35 counts.
! >100%: ± 3.5% of the reading (>5°C to ≤ 55°C).
o Concentration:
! ± 0.35 ppm or ± 3.5 % of the reading, whichever is greater (up to 40˚C).
• TEMPERATURE CONTROLLED, FULL CALIBRATION @ >5°C to ≤ 55°C:
o Saturation:
! 0-100.0%: ± 20 counts; >100%: ± 2% of the reading (>5°C to ≤ 55°C).
o Concentration:
! ±0.2 ppm or ±2% of the reading, whichever is greater (up to 40˚C).
II. SENSOR CHECK
The Sensor Check is a built in feature to check the amplitude of the signal from the sensor. This is used as a troubleshooting
tool only, and is not designed to detect all sources of failure.
1. Remove the protective / hydration cap from the DO sensor.
2. Dip the sensor in clean DI, RO, or purified water.
3. CAREFULLY blot the sensor membrane with a soft, clean, lint-free cloth or tissue to remove any water drops.
4. Fill the protective / hydration cap with enough DI, RO, or distilled water to soak the sponge inside.
5. Hold the PTBT5 upright with the DO sensor down and insert the sensor into the cap. Make sure there is a good seal
between the sensor and the cap.
• DO NOT insert the sensor so far that the vent hole is covered.
6. Press and release the PEN BUTTON on the PTBT5 to turn it ON and place the PTBT5 into Standby mode.
7. Tap the CAL button in the Feature Navigation Bar.
12. The value displayed will include
temperature compensation, but
no calibration constant will have
been applied.
13. The Sensor is GOOD, if the
value displayed is ≥ 57.5%.
NOTE: If the result is <57.5%, the
sensor is not in optimal
condition. The sensor may need
to be cleaned, the membrane
cap assembly may need to be
replaced, or the entire sensor
may need to be replaced (See
MAINTENANCE section below).
14. Tap the DONE button to return
to the main calibration screen.
8. The Initial CAL screen will
appear.
9. Tap the CAL button.
10. On the Secondary CAL
screen, tap the Sensor Check
button.
11. The App will display the
raw DO Saturation value.