Okolab H401-T User manual

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H401-T-CONTROLLER
Temperature Controller
Manual
IST747_REV01
SV 2.3.8.2

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Index
Index
1PREFACE .....................................................................................................................................................................................4
2SYMBOL DESCRIPTION ..........................................................................................................................................................5
2.1 SYMBOLS USED IN THIS MANUAL...............................................................................................................................................................5
2.2 SYMBOLS ON THE PRODUCT LABEL ...........................................................................................................................................................5
3SAFETY NOTES..........................................................................................................................................................................6
4SUPPLIED EQUIPMENT..........................................................................................................................................................7
5OPTIONAL EQUIPMENT.........................................................................................................................................................7
6EQUIPMENT DESCRIPTION ..................................................................................................................................................8
7INSTALLATION .........................................................................................................................................................................8
7.1 COMPATIBILITY............................................................................................................................................................................................9
7.2 CONNECTIONS OF THE HEATING DEVICES.............................................................................................................................................10
7.3 MINI-USB PORT ......................................................................................................................................................................................11
7.3.1 Connection of a USB drive .......................................................................................................................................................11
7.3.2 PC Connection via USB..............................................................................................................................................................11
7.4 RS232 PORT –PC CONNECTION VIA RS232......................................................................................................................................12
7.5 ALARM SCREW TERMINAL .......................................................................................................................................................................13
8USER INTERFACE .................................................................................................................................................................. 14
8.1 HOMEPAGE.................................................................................................................................................................................................14
8.1.1 How to enter the set point temperature...........................................................................................................................15
8.1.2 Controller Status: colours and meaning............................................................................................................................16
8.2 SETTINGS....................................................................................................................................................................................................17
8.2.1 Device configuration..................................................................................................................................................................17
8.2.1.1 Device Type.........................................................................................................................................................................................................18
8.2.1.2 Device Label .......................................................................................................................................................................................................18
8.2.1.3 Device Maximum Heating Ramp Rate ....................................................................................................................................................19
8.2.2 Calibration routines ...................................................................................................................................................................20
8.2.2.1 Heating Device Calibration ......................................................................................................................................................................... 20
8.2.2.2 T Sensor Calibration.......................................................................................................................................................................................27
8.2.3 Data Logging..................................................................................................................................................................................29
8.2.4 Alarms..............................................................................................................................................................................................32
8.2.5 Display configuration ................................................................................................................................................................34
8.2.5.1 Display Options.................................................................................................................................................................................................34
8.2.5.2 Display Brightness...........................................................................................................................................................................................35
8.2.5.3 Display Calibration ......................................................................................................................................................................................... 35
8.2.5.4 Date & Time........................................................................................................................................................................................................ 36
8.2.5.5 Display Visual Effects –icon and glance mode ...................................................................................................................................36
8.2.6 Password.........................................................................................................................................................................................37
8.2.7 Summary.........................................................................................................................................................................................38
8.3 OVERVIEW STATUS PAGE ........................................................................................................................................................................39
8.4 ICON AND GLANCE MODE VIEW.............................................................................................................................................................39
8.5 INFO PAGE ..................................................................................................................................................................................................40
9CLEANING & MAINTENANCE............................................................................................................................................. 40
10 H401-T-CONTROLLER-OEM.......................................................................................................................................... 41
10.1 DESCRIPTION.............................................................................................................................................................................................41
10.2 EQUIPMENT SUPPLIED.............................................................................................................................................................................41
10.3 INSTALLATION...........................................................................................................................................................................................42
10.3.1 Connections of the heating devices.....................................................................................................................................44
10.3.2 T sensor Connection ..................................................................................................................................................................45

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10.3.3 MINI-USB port ..............................................................................................................................................................................46
10.3.3.1 Connection of a USB drive............................................................................................................................................................................ 47
10.3.3.2 PC Connection via USB................................................................................................................................................................................... 47
10.3.4 Alarm Screw Terminal..............................................................................................................................................................47
11 SUPPORT ............................................................................................................................................................................. 48
12 TECHNICAL SPECIFICATIONS ....................................................................................................................................... 50
13 TROUBLESHOOTING........................................................................................................................................................ 51
14 FIGURE LIST........................................................................................................................................................................ 52
15 TABLE LIST ......................................................................................................................................................................... 53
16 MANUAL REVISION TABLE ............................................................................................................................................ 53
17 LIST OF SPARE PARTS FOR H401-T-CONTROLLER AND H401-T-CONTROLLER-OEM ........................... 53

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1Preface
The H401-T-CONTROLLER is a touch screen digital dual channel temperature controller and data log
device allowing temperature set up, control and logging of two separate heating devices. It is a compact and
reliable solution to maintain the effective desired temperature of the specimen right on the microscope stage
within the range 25-60°C. The H401-T-CONTROLLER allows to set two different temperatures for the two
devices.
The H401-T-CONTROLLER comprises the Temperature Controller and a miniaturized water / oil
immersible temperature sensor. The temperature sensor allows to calibrate the heating devices with great
accuracy, by providing feedback from the temperature of the culture medium contained inside the dish. The
heating devices must be purchased separately.
A variety of heating devices can be connected to the H401-T-CONTROLLER, for example: heating plates -
both glass and metal for inverted, upright and stereo microscopes, glass heating tables, metal heating pads, stage
heating pads or objective heaters. The heating plates –both glass and metal- are designed to fit into the opening
of XY stages of upright and inverted microscopes. Several models of glass and metal heating plate inserts can be
selected from the Okolab portfolio. Custom solutions are available upon request. The glass heating table can be
placed in the optical path of any stereomicroscope and provides a large, flat, clear, warm surface where one or
more specimens can be maintained at physiological temperature. The glass heating table is also suitable for small
animal surgery. The metal heating pads are temperature-controlled surfaces that can be positioned on any
laboratory workbench or desk surface (e.g. table top, cabinet work surface, etc.) to keep Petri dishes, slides, and
tubes at the right temperature before microscope observation. The stage heating pads clamp on the XY stage and
create a temperature-controlled, flat surface on the entire stage, with the exception of the central part, which can
be covered with a glass or metal heating insert. The objective heater is a flexible heated band designed to
maintain the desired temperature of the objective body and lenses. This is important when the objective is in
contact with the sample during water dipping or oil immersion observation.
The H401-T-CONTROLLER features an intuitive, user-friendly touch screen interface that enables you to
perform accurate calibrations in an efficient and simple manner.
The H401-T-CONTROLLER is also equipped with on-board memory for data logging and storage. A
simple routine allows to download data to USB drive or to PC.
We recommend reading carefully this manual to familiarize yourself with the functions and the
operation of H401-T-CONTROLLER before use.

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2Symbol description
This paragraph describes the symbols used in the manual and on the product label.
2.1 Symbols used in this manual
The following symbols identify important information to note:
CAUTION or WARNING: this symbol warns you about the risk of electrical shock.
CAUTION or WARNING or IMPORTANT: this symbol warns you of circumstances or practices that
can affect the functionality of the instrument.
Tip ►Supplies you with helpful suggestions.
Note ►Supplies you with important information to successfully setup and use the instrument.
2.2 Symbols on the product label
CE MARKING: this symbol indicates product compliance with EU legislation.
PRODUCT DISPOSAL: this symbol indicates that this product must not be disposed as urban
solid waste.
This symbol indicates the product production date.
This symbol indicates the manufacturer data.
This symbol indicates the protection degree against ingress of solids or liquids inside the
product.
This symbol warns you to read the user manual before starting the device.

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3Safety Notes
In order to achieve maximum performance and to ensure proper operation of your new equipment,
please read carefully the following safety notes and the instructions. If you have any question, please contact
Okolab.
The equipment must only be used as intended and as described in this Manual.
Equipment should be operated only by technically qualified personnel.
Do not start up the equipment if some of its parts are damaged.
This instrument is not intended for use in locations subject to flammable or explosive gases.
Transport the equipment with care.
Equipment and its internal parts can be damaged by dropping and by shock.
Some equipment parts may reach temperatures above 60°C. Take care when touching it.
Avoid rapid changes in ambient temperature which may cause condensation, avoid direct air
draft from air conditioner, exposure to direct sunlight and excessive heat accumulation.
Do not disassemble any part of the system.
Do not disconnect cables while in operation.
Do not use a volatile solvent such as paint thinner to clean the instrument because deformation
or discoloration may occur.
Use a soft, dry cloth to remove stains from the instrument.
Do not exceed voltage indicated in this manual and on the product label.
Avoid excessive induction noise, static electricity and magnetic fields.
Do not expose this instrument to rain or moisture.
Prevent throttling and kinking of cables.
Do not start up the equipment if the supply cable is damaged.
Connect the equipment only to grounded mains power socket.
Before starting, assemble the equipment while unplugged from an outlet.
Prevent metal fragments or lead wire scraps from falling inside instrument case to avoid
electric shock, fire or malfunction.
International caution symbol marks this device. IMPORTANT: read the “Safety Notes” before
installing, using and commissioning this device, as the notes contain important information relating
to safety and EMC. Not following these instructions can result in damage or breakdown of the
device and its accessories.
We reserve the right to make technical modifications.
IN NO EVENT OKOLAB S.R.L. SHALL BE LIABLE FOR ANY DIRECT, INCIDENTAL OR CONSEQUENTIAL
DAMAGES OF ANY NATURE, OR FINANCIAL LOSS RESULTING FROM IMPROPER USE OF THE PRODUCT.

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4Supplied equipment
1Controller. Dual channel temperature controller. See pointer 1 in Figure 1.
2T Sensor. Fine gauge immersible thermocouple, which can be used to calibrate the heating devices
connected to the Controller and to meter temperature, as needed. See pointer 2 in Figure 1.
3Power feeder and power cord. 24V DC-60 W power feeder. See pointer 3 in Figure 1.
4MINI-USB-OTG. Mini USB OTG cable to connect a USB drive to the H401-T-CONTROLLER for data
download. See pointer 4 in Figure 1.
5Alarm screw terminal. Contact closure alarm. See pointer 5 in Figure 1.
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3
1
2
5
Figure 1. H401-T-CONTROLLER –Components.
5Optional Equipment
1DATA LOG (Optional-to be purchased separately).It is a program that allows to use in tethered mode or
to log any Okolab device using a PC.
Figure 2. DATA LOG software.

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6Equipment Description
The H401-T-CONTROLLER is a touch screen digital dual channel temperature controller and data log
device allowing temperature set up, control and logging of two separate heating devices. It is a compact and
effective solution to reliably maintain the desired temperature of the specimen right on the microscope stage
within the range 25-60°C. The H401-T-CONTROLLER allows to set two different temperatures for the two
devices.
The H401-T-CONTROLLER comprises the Temperature Controller and a miniaturized water / oil
immersible temperature sensor which allows to calibrate the heating devices with great accuracy, by providing
feedback from the temperature of the culture medium contained inside the dish. The heating devices must be
purchased separately.
A detailed description of the H401-T-CONTROLLER is provided in the following list, which refers to the
pointers in Figure 3:
124 VDC Power Input
2Device 1 port
3Device 2 port
4External Alarm Contact Closure
5T Sensor port
6Mini USB port
7Serial port
8Power switch
5
6
8
1
24
3
7
Figure 3. H401-T-CONTROLLER –Equipment description.
7Installation
H401-T-CONTROLLER can control the temperature of up to two heating devices at the same time. Set
point temperature of the heating devices can be different.

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7.1 Compatibility
H401-T-CONTROLLER is compatible with the following Okolab heating devices:
H401-T-GLASS series
H401-T-METAL series
H401-T-PAD series
H401-STAGE-T-PAD series
H401-GLASS-TABLE
OBJ-COLLAR-SERIES
For a complete list of compatible heating devices, refer to: www.oko-lab.com.

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7.2 Connections of the heating devices
Do not turn on the device until you have completed and checked all connections.
Follow the instructions below to connect the heating devices to H401-T-CONTROLLER.
24 VDC
Power Feeder Device1 Connector
Device2 Connector
Figure 4. Connection of the heating devices
1Connect one heating device to the Device 1 connector on the rear panel of H401-T-CONTROLLER (see
Figure 4 and pointer 2 in Figure 3).
2Connect the other heating device (if available) to the Device 2 connector on the rear panel of the H401-T-
CONTROLLER (see Figure 4 and pointer 3 in Figure 3).
3Connect the T Sensor to the T Sensor connector on the rear panel of H401-T-CONTROLLER (see Figure 4
and pointer 5 of Figure 3).
4Connect the Power Feeder to the Power Input (see Figure 4).
Tip ►Okolab provides the correct power adapter compatible with the devices configuration selected by
the user in the purchase order. The majority of configurations use a power adapter with Rating Power of 60
W. The configurations reported in Table 1 require a power adapter with Rating Power of 120 W.
Heating devices connected to the H401-T-CONTROLLER
Power required
#2 H401-T-PAD
120 W
#2 H401-GLASS-TABLE
120 W
#2 H401-T-STAGE-PAD
120 W
#1 H401-GLASS-TABLE+#1 H401-T-PAD
120 W
#1 H401-GLASS-TABLE+#1 H401-T-STAGE-PAD
120 W
#1 H401-T-PAD+#1 H401-T-STAGE-PAD
120 W
Any other configuration
60 W
Table 1. Heating devices that require power adapter with Rating Power 120 W.