Okolab H201-T-UNIT-BL User manual

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H201-T-UNIT-BL
Temperature Control Unit
Manual
IST1254_REV01
SV 3.5.4.3048

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Index
1PREFACE .....................................................................................................................................................................................3
2SYMBOL DESCRIPTION ..........................................................................................................................................................4
SYMBOLS USED IN THIS MANUAL...............................................................................................................................................................4
SYMBOLS ON THE PRODUCT LABEL ...........................................................................................................................................................4
3SAFETY NOTES..........................................................................................................................................................................5
4SUPPLIED EQUIPMENT..........................................................................................................................................................7
5NOT SUPPLIED EQUIPMENT ................................................................................................................................................7
OPTIONAL EQUIPMENT...............................................................................................................................................................................8
6EQUIPMENT DESCRIPTION ..................................................................................................................................................9
7INSTALLATION ...................................................................................................................................................................... 11
H201-T-UNIT-BL. INSTALLATION GUIDE .........................................................................................................................................11
HOW TO CONNECT H201-T-UNIT-BL TO A BOLD LINE DIGITAL GAS CONTROLLER.............................................................13
8USER INTERFACE .................................................................................................................................................................. 14
HOME PAGE................................................................................................................................................................................................14
8.1.1 How to enter the set point temperature ...........................................................................................................................14
8.1.2 Controller Status: colours led and meaning....................................................................................................................15
SETTINGS....................................................................................................................................................................................................16
8.2.1 Temperature .................................................................................................................................................................................16
8.2.1.1 Control Mode ..................................................................................................................................................................................................... 17
8.2.1.2 Fan Speed ............................................................................................................................................................................................................19
8.2.1.3 Air filters..............................................................................................................................................................................................................20
8.2.1.4 Status ....................................................................................................................................................................................................................22
8.2.1.5 Calibration..........................................................................................................................................................................................................23
8.2.1.6 Digital Input.......................................................................................................................................................................................................23
8.2.2 Touch Screen.................................................................................................................................................................................24
8.2.2.1 Options..................................................................................................................................................................................................................24
8.2.2.2 Brightness ........................................................................................................................................................................................................... 25
8.2.2.3 Visual Effects...................................................................................................................................................................................................... 25
8.2.2.4 Date & Time........................................................................................................................................................................................................ 25
8.2.2.5 Alarms...................................................................................................................................................................................................................26
8.2.2.6 Data Logging..................................................................................................................................................................................................... 27
OVERVIEW..................................................................................................................................................................................................31
ICON AND GLANCE MODE VIEW.............................................................................................................................................................31
INFO PAGE ..................................................................................................................................................................................................31
9CALIBRATION......................................................................................................................................................................... 32
CAGE-CALIBRATION .................................................................................................................................................................................32
10 ENCLOSURE STANDARD COMPONENTS ................................................................................................................... 34
11 CLEANING & MAINTENANCE......................................................................................................................................... 42
CONTROL UNIT..........................................................................................................................................................................................42
ENCLOSURE................................................................................................................................................................................................42
FILTERS REPLACEMENT ..........................................................................................................................................................................43
12 SUPPORT ............................................................................................................................................................................. 45
13 TECHNICAL SPECIFICATIONS ....................................................................................................................................... 46
14 TROUBLESHOOTING........................................................................................................................................................ 47
15 FIGURE LIST........................................................................................................................................................................ 48
16 MANUAL REVISION TABLE ............................................................................................................................................ 49

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1Preface
The H201-T-UNIT-BL, together with the H201-ENCLOSURE, is designed to maintain all the thermal
conditions required around your microscope, thus enabling to carry out prolonged observations on biological
specimens. The system controls the temperature in the range of 25-45°C.
The system is composed of the enclosure surrounding the microscope, H201-ENCLOSURE, which design
depends on the user’s microscope, the Temperature Control Unit, H201-T-UNIT-BL with the Heating Box, the HM-
VF composed by a humidity module and a water trap (purchased separately)
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, a fine gauge thermocouple to
monitor the temperature of a blank sample or Reference Well located in close proximity to the experimental one,
a thermistor to monitor the cage temperature or the Room Temperature and the OKO-TOUCH touch screen control
panel (to be ordered separately).
H201-T-UNIT-BL is compatible with any Bold Line Gas Controller Temperature and Gas controllers are
stackable and connectable through a bus connector (see paragraph 7.1).
H201-T-UNIT-BL is operated via the OKO-TOUCH touch screen control panel (to be ordered separately).
OKO-TOUCH features on-board memory for data logging and mini-USB port for data download.
In addition data logging is possible via SMART-BOX, via DATA-LOG Okolab software and via following
third-party Imaging software: LASX 2.0, NIS-Elements, SlideBook, MetaMorph and MicroManager. Macro for ZEN
Black software is available upon request.
Okolab DATA LOG is an optional software allowing logging temperature data or modification of selected
parameters. DATA LOG can be installed on your PC; a mini USB connection to the Temperature Control Unit allows
data download.
Okolab Bold Line controllers are also capable to receive TTL input (requires to order separately TTL-
CABLE).
The H201-T-UNIT-BL can be equipped with the H201-T-FILTER-BOX (optional) that assembles with the
Heating Box to ensure a dust and bacteria free environment inside the enclosure. In this case, H201-T-FILTER-BOX
is assembled with the H201-FILTER KIT, a kit composed by a Prefilter and a HEPA filter (see Figure 20 in paragraph
8.2.1.3).
We recommend reading carefully this manual to familiarize yourself with the functions and the operation
of H201-T-UNIT-BL before use.
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As Humidity Module you can purchase also HM-ACTIVE or HM-STANDALONE systems. In this case please refer to their own
manual for humidity-related instructions.

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2Symbol description
This paragraph describes the symbols used in the manual and on the product label.
Symbols used in this manual
The following symbols identify important information to note:
CAUTION or WARNING: this symbol warns you about the risk of electrical shock.
CAUTION or WARNING or IMPORTANT: this symbol warns you of circumstances or practices that
can affect the functionality of the instrument.
Tip ►Supplies you with helpful suggestions.
Note ►Supplies you with important information to successfully setup and use the instrument.
Symbols on the product label
CE MARKING: this symbol indicates product compliance with EU legislation.
PRODUCT DISPOSAL: this symbol indicates that this product must not be disposed as urban solid
waste.
This symbol indicates the product production date.
This symbol indicates the manufacturer data.
This symbol warns you to read the user manual before starting the device.
This symbol indicates the protection degree against ingress of solids or liquids inside the product.
IP 30

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3Safety Notes
In order to achieve maximum performance and to ensure proper operation of your new equipment, please
read carefully the following safety notes and the instructions. If you have any question, please contact Okolab.
−The equipment must only be used as intended and as described in this Manual.
−Equipment should be operated only by technically qualified personnel.
−Do not start up the equipment if some of its parts are damaged.
−This instrument is not intended for use in locations subject to flammable or explosive gases.
−Transport the equipment with care.
−Equipment and its internal parts can be damaged by dropping and by shock.
−Not following these instructions can result in damage or breakdown of the device and its
accessories.
−Avoid rapid changes in ambient temperature which may cause condensation, avoid direct air
draft from air conditioner, exposure to direct sunlight and excessive heat accumulation.
−The products labels can be found on the bottom panel of the Main Unit.
−Do not disassemble any part of the system.
−Do not disconnect cables while in operation.
−Do not use a volatile solvent such as paint thinner to clean the instrument because deformation
or discoloration may occur.
−Use a soft, dry cloth to remove stains from the instrument.
−Do not exceed voltage indicated in this manual and on the product label.
−Avoid excessive induction noise, static electricity and magnetic fields.
−Do not expose this instrument to rain or moisture.
−Prevent throttling and kinking of tubing.
−Check tubing time to time for possible material usage.
−Check that all tubing is well inserted into the connectors so they cannot slip off
−This device is not designed for use for medical applications.
−Power cord of unit should be unplugged from electrical outlet when left unused for a long period
of time.
−Do not start up the equipment if the supply cable is damaged.
−Connect the equipment only to grounded mains power socket.
−Do not open the unit. Do not remove cover or back.
−Prevent metal fragments or lead wire scraps from falling inside instrument to avoid electric
shock, fire or malfunction.
−No user serviceable parts inside.
−Unit should never be used where it can fall or be pushed into water.

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International caution symbol marks this device. It is important to read the “Safety Notes” before
installing, using and commissioning this device, as the notes contain important information relating
to safety and EMC. Not following these instructions can result in damage or breakdown of the device
and its accessories.
We reserve the right to make technical variations.
IN NO EVENT SHALL OKOLAB S.R.L. BE LIABLE FOR ANY DIRECT, INCIDENTAL OR CONSEQUENTIAL
DAMAGES OF ANY NATURE, OR LOSSES OR EXPENSES RESULTING FROM ANY DEFECTIVE PRODUCT OR THE USE
OF ANY PRODUCT.

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4Supplied equipment
H201-T-UNIT-BL includes:
1. T-Unit (pointer 1 in Figure 1)
2. Heating Box (pointer 2 in Figure 1)
3. Fine Gauge Thermocouple (pointer 3 in Figure 1). To measure specimen temperature
4. NTC Thermistor (pointer 4 in Figure 1). To measure ambient temperature
5. #1 24V-DC Power Adapter (pointer 5 in Figure 1). To connect the Temperature Control Unit
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6
1
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7
12
13
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Figure 1. H201-T-UNIT-BL equipment overview.
5NOT supplied equipment
H201-T-UNIT-BL can be equipped with:
1. Microscope enclosure (pointer 6 in Figure 1)
2. HM-VF, standard humidity control, that includes:
•Humidity Module (pointer 7 in Figure 1)
•Water Trap (see paragraph 10 Figure 56)
•TUBE G (x1). Silicon tube, 3 mm ID, connected by a plastic connector with a silicon tube 2 mm ID. The
3 mm ID silicone end must be connected to the Output Water Trap, while the 2 mm ID silicone tube to
the Chamber Incubator. See Figure 66.
•TUBE H (x1). Silicon tube, 3 mm ID. To connect the Humidity Module Output to the Water Trap Input.
See Figure 66.
•TUBE I (x1): Polyurethane tube, 4 mm OD, 45 mm long. To connect the Input of the gas coming from
the Gas Mixer to the Humidity Module. See Figure 66.

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3. 8-pins cable (x1). To connect the connector labeled ‘Control Unit’ on the H201 T Heating Box with the
connector labeled ‘Heating Box’ on the Temperature Control Unit (see paragraph7.1).
4. 3-pins –Flow Sensor Cable (x1). To connect the flow sensor attached on the Blue Side of Heating Box
to the connector labeled ‘Air flow sensor’ on the Temperature Control Unit (see paragraph 7.1).
5. Flexible tubes, TUBE J (x4) (pointer 10 in Figure 1). To connect the Heating Box to the enclosure (two
tubes to attach the RED side of the Heating Box to hoses on the BACK of the enclosure and other two
tubes to attach the BLUE side of the Heating Box to hoses on the TOP of the enclosure) (see paragraph 10
Figure 57 and Figure 58).
6. Power supply cable. To connect the Heating Box.
7. Okolab incubating chamber (see pointer 12 in Figure 1) with lid and base, chamber riser with one or
more plate adapters.
8. OKO-TOUCH (see point 13 in Figure 1), including:
−Touch Screen Interface
−#1 RS232 Serial cable. To connect the Touch Screen to the T-Unit
−MINI-USB-OTG cable. To connect a USB pen to the OKO-TOUCH for data download.
Touch Screen Interface
RS232 Serial cable
Figure 2. Oko-touch components.
Optional Equipment
•H201-T-FILTER-BOX , including:
1. H201-T-FILTER-KIT, Pre filter and HEPA filter

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H201-T-FILTER-BOX
Figure 3. H201-T-FILTER-BOX.
•DATA LOG (Optional-to be purchased separately). Software used to log, view, and store data from a
PC. It may be used to change parameters of the controllers via PC. Data can be exported in .xlsx format.
Figure 4. DATA LOG software.
•SM-BL Smart Box. To store data, to operate the controllers via web/smartphone/tablet and to allow
remote assistance for service.
6Equipment Description
A detailed description of the H201-T-UNIT-BL rear is provided in the following list, which refers to the
pointers in Figure 5:
1. OKO-TOUCH RS232 serial port.
2. Power Input.
3. Heating Box connector.
4. Air Flow Sensor connector.
5. USB port.
6. General Purpose Input Output (GPIO) port.
7. NTC Thermistor port.

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8. Gauge Thermocouple port.
2354 6 7 8
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Figure 5. Control Unit Overview