Okolab H201-T-UNIT-BL User manual

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H201-T-UNIT-BL
Temperature Control Unit
Manual
IST1254_REV01
SV 3.5.4.3048

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Index
1PREFACE .....................................................................................................................................................................................3
2SYMBOL DESCRIPTION ..........................................................................................................................................................4
SYMBOLS USED IN THIS MANUAL...............................................................................................................................................................4
SYMBOLS ON THE PRODUCT LABEL ...........................................................................................................................................................4
3SAFETY NOTES..........................................................................................................................................................................5
4SUPPLIED EQUIPMENT..........................................................................................................................................................7
5NOT SUPPLIED EQUIPMENT ................................................................................................................................................7
OPTIONAL EQUIPMENT...............................................................................................................................................................................8
6EQUIPMENT DESCRIPTION ..................................................................................................................................................9
7INSTALLATION ...................................................................................................................................................................... 11
H201-T-UNIT-BL. INSTALLATION GUIDE .........................................................................................................................................11
HOW TO CONNECT H201-T-UNIT-BL TO A BOLD LINE DIGITAL GAS CONTROLLER.............................................................13
8USER INTERFACE .................................................................................................................................................................. 14
HOME PAGE................................................................................................................................................................................................14
8.1.1 How to enter the set point temperature ...........................................................................................................................14
8.1.2 Controller Status: colours led and meaning....................................................................................................................15
SETTINGS....................................................................................................................................................................................................16
8.2.1 Temperature .................................................................................................................................................................................16
8.2.1.1 Control Mode ..................................................................................................................................................................................................... 17
8.2.1.2 Fan Speed ............................................................................................................................................................................................................19
8.2.1.3 Air filters..............................................................................................................................................................................................................20
8.2.1.4 Status ....................................................................................................................................................................................................................22
8.2.1.5 Calibration..........................................................................................................................................................................................................23
8.2.1.6 Digital Input.......................................................................................................................................................................................................23
8.2.2 Touch Screen.................................................................................................................................................................................24
8.2.2.1 Options..................................................................................................................................................................................................................24
8.2.2.2 Brightness ........................................................................................................................................................................................................... 25
8.2.2.3 Visual Effects...................................................................................................................................................................................................... 25
8.2.2.4 Date & Time........................................................................................................................................................................................................ 25
8.2.2.5 Alarms...................................................................................................................................................................................................................26
8.2.2.6 Data Logging..................................................................................................................................................................................................... 27
OVERVIEW..................................................................................................................................................................................................31
ICON AND GLANCE MODE VIEW.............................................................................................................................................................31
INFO PAGE ..................................................................................................................................................................................................31
9CALIBRATION......................................................................................................................................................................... 32
CAGE-CALIBRATION .................................................................................................................................................................................32
10 ENCLOSURE STANDARD COMPONENTS ................................................................................................................... 34
11 CLEANING & MAINTENANCE......................................................................................................................................... 42
CONTROL UNIT..........................................................................................................................................................................................42
ENCLOSURE................................................................................................................................................................................................42
FILTERS REPLACEMENT ..........................................................................................................................................................................43
12 SUPPORT ............................................................................................................................................................................. 45
13 TECHNICAL SPECIFICATIONS ....................................................................................................................................... 46
14 TROUBLESHOOTING........................................................................................................................................................ 47
15 FIGURE LIST........................................................................................................................................................................ 48
16 MANUAL REVISION TABLE ............................................................................................................................................ 49

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1Preface
The H201-T-UNIT-BL, together with the H201-ENCLOSURE, is designed to maintain all the thermal
conditions required around your microscope, thus enabling to carry out prolonged observations on biological
specimens. The system controls the temperature in the range of 25-45°C.
The system is composed of the enclosure surrounding the microscope, H201-ENCLOSURE, which design
depends on the user’s microscope, the Temperature Control Unit, H201-T-UNIT-BL with the Heating Box, the HM-
VF composed by a humidity module and a water trap (purchased separately)
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, a fine gauge thermocouple to
monitor the temperature of a blank sample or Reference Well located in close proximity to the experimental one,
a thermistor to monitor the cage temperature or the Room Temperature and the OKO-TOUCH touch screen control
panel (to be ordered separately).
H201-T-UNIT-BL is compatible with any Bold Line Gas Controller Temperature and Gas controllers are
stackable and connectable through a bus connector (see paragraph 7.1).
H201-T-UNIT-BL is operated via the OKO-TOUCH touch screen control panel (to be ordered separately).
OKO-TOUCH features on-board memory for data logging and mini-USB port for data download.
In addition data logging is possible via SMART-BOX, via DATA-LOG Okolab software and via following
third-party Imaging software: LASX 2.0, NIS-Elements, SlideBook, MetaMorph and MicroManager. Macro for ZEN
Black software is available upon request.
Okolab DATA LOG is an optional software allowing logging temperature data or modification of selected
parameters. DATA LOG can be installed on your PC; a mini USB connection to the Temperature Control Unit allows
data download.
Okolab Bold Line controllers are also capable to receive TTL input (requires to order separately TTL-
CABLE).
The H201-T-UNIT-BL can be equipped with the H201-T-FILTER-BOX (optional) that assembles with the
Heating Box to ensure a dust and bacteria free environment inside the enclosure. In this case, H201-T-FILTER-BOX
is assembled with the H201-FILTER KIT, a kit composed by a Prefilter and a HEPA filter (see Figure 20 in paragraph
8.2.1.3).
We recommend reading carefully this manual to familiarize yourself with the functions and the operation
of H201-T-UNIT-BL before use.
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As Humidity Module you can purchase also HM-ACTIVE or HM-STANDALONE systems. In this case please refer to their own
manual for humidity-related instructions.

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2Symbol description
This paragraph describes the symbols used in the manual and on the product label.
Symbols used in this manual
The following symbols identify important information to note:
CAUTION or WARNING: this symbol warns you about the risk of electrical shock.
CAUTION or WARNING or IMPORTANT: this symbol warns you of circumstances or practices that
can affect the functionality of the instrument.
Tip ►Supplies you with helpful suggestions.
Note ►Supplies you with important information to successfully setup and use the instrument.
Symbols on the product label
CE MARKING: this symbol indicates product compliance with EU legislation.
PRODUCT DISPOSAL: this symbol indicates that this product must not be disposed as urban solid
waste.
This symbol indicates the product production date.
This symbol indicates the manufacturer data.
This symbol warns you to read the user manual before starting the device.
This symbol indicates the protection degree against ingress of solids or liquids inside the product.
IP 30

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3Safety Notes
In order to achieve maximum performance and to ensure proper operation of your new equipment, please
read carefully the following safety notes and the instructions. If you have any question, please contact Okolab.
−The equipment must only be used as intended and as described in this Manual.
−Equipment should be operated only by technically qualified personnel.
−Do not start up the equipment if some of its parts are damaged.
−This instrument is not intended for use in locations subject to flammable or explosive gases.
−Transport the equipment with care.
−Equipment and its internal parts can be damaged by dropping and by shock.
−Not following these instructions can result in damage or breakdown of the device and its
accessories.
−Avoid rapid changes in ambient temperature which may cause condensation, avoid direct air
draft from air conditioner, exposure to direct sunlight and excessive heat accumulation.
−The products labels can be found on the bottom panel of the Main Unit.
−Do not disassemble any part of the system.
−Do not disconnect cables while in operation.
−Do not use a volatile solvent such as paint thinner to clean the instrument because deformation
or discoloration may occur.
−Use a soft, dry cloth to remove stains from the instrument.
−Do not exceed voltage indicated in this manual and on the product label.
−Avoid excessive induction noise, static electricity and magnetic fields.
−Do not expose this instrument to rain or moisture.
−Prevent throttling and kinking of tubing.
−Check tubing time to time for possible material usage.
−Check that all tubing is well inserted into the connectors so they cannot slip off
−This device is not designed for use for medical applications.
−Power cord of unit should be unplugged from electrical outlet when left unused for a long period
of time.
−Do not start up the equipment if the supply cable is damaged.
−Connect the equipment only to grounded mains power socket.
−Do not open the unit. Do not remove cover or back.
−Prevent metal fragments or lead wire scraps from falling inside instrument to avoid electric
shock, fire or malfunction.
−No user serviceable parts inside.
−Unit should never be used where it can fall or be pushed into water.

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International caution symbol marks this device. It is important to read the “Safety Notes” before
installing, using and commissioning this device, as the notes contain important information relating
to safety and EMC. Not following these instructions can result in damage or breakdown of the device
and its accessories.
We reserve the right to make technical variations.
IN NO EVENT SHALL OKOLAB S.R.L. BE LIABLE FOR ANY DIRECT, INCIDENTAL OR CONSEQUENTIAL
DAMAGES OF ANY NATURE, OR LOSSES OR EXPENSES RESULTING FROM ANY DEFECTIVE PRODUCT OR THE USE
OF ANY PRODUCT.

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4Supplied equipment
H201-T-UNIT-BL includes:
1. T-Unit (pointer 1 in Figure 1)
2. Heating Box (pointer 2 in Figure 1)
3. Fine Gauge Thermocouple (pointer 3 in Figure 1). To measure specimen temperature
4. NTC Thermistor (pointer 4 in Figure 1). To measure ambient temperature
5. #1 24V-DC Power Adapter (pointer 5 in Figure 1). To connect the Temperature Control Unit
2
6
1
34
5
7
12
13
10
Figure 1. H201-T-UNIT-BL equipment overview.
5NOT supplied equipment
H201-T-UNIT-BL can be equipped with:
1. Microscope enclosure (pointer 6 in Figure 1)
2. HM-VF, standard humidity control, that includes:
•Humidity Module (pointer 7 in Figure 1)
•Water Trap (see paragraph 10 Figure 56)
•TUBE G (x1). Silicon tube, 3 mm ID, connected by a plastic connector with a silicon tube 2 mm ID. The
3 mm ID silicone end must be connected to the Output Water Trap, while the 2 mm ID silicone tube to
the Chamber Incubator. See Figure 66.
•TUBE H (x1). Silicon tube, 3 mm ID. To connect the Humidity Module Output to the Water Trap Input.
See Figure 66.
•TUBE I (x1): Polyurethane tube, 4 mm OD, 45 mm long. To connect the Input of the gas coming from
the Gas Mixer to the Humidity Module. See Figure 66.

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3. 8-pins cable (x1). To connect the connector labeled ‘Control Unit’ on the H201 T Heating Box with the
connector labeled ‘Heating Box’ on the Temperature Control Unit (see paragraph7.1).
4. 3-pins –Flow Sensor Cable (x1). To connect the flow sensor attached on the Blue Side of Heating Box
to the connector labeled ‘Air flow sensor’ on the Temperature Control Unit (see paragraph 7.1).
5. Flexible tubes, TUBE J (x4) (pointer 10 in Figure 1). To connect the Heating Box to the enclosure (two
tubes to attach the RED side of the Heating Box to hoses on the BACK of the enclosure and other two
tubes to attach the BLUE side of the Heating Box to hoses on the TOP of the enclosure) (see paragraph 10
Figure 57 and Figure 58).
6. Power supply cable. To connect the Heating Box.
7. Okolab incubating chamber (see pointer 12 in Figure 1) with lid and base, chamber riser with one or
more plate adapters.
8. OKO-TOUCH (see point 13 in Figure 1), including:
−Touch Screen Interface
−#1 RS232 Serial cable. To connect the Touch Screen to the T-Unit
−MINI-USB-OTG cable. To connect a USB pen to the OKO-TOUCH for data download.
Touch Screen Interface
RS232 Serial cable
Figure 2. Oko-touch components.
Optional Equipment
•H201-T-FILTER-BOX , including:
1. H201-T-FILTER-KIT, Pre filter and HEPA filter

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H201-T-FILTER-BOX
Figure 3. H201-T-FILTER-BOX.
•DATA LOG (Optional-to be purchased separately). Software used to log, view, and store data from a
PC. It may be used to change parameters of the controllers via PC. Data can be exported in .xlsx format.
Figure 4. DATA LOG software.
•SM-BL Smart Box. To store data, to operate the controllers via web/smartphone/tablet and to allow
remote assistance for service.
6Equipment Description
A detailed description of the H201-T-UNIT-BL rear is provided in the following list, which refers to the
pointers in Figure 5:
1. OKO-TOUCH RS232 serial port.
2. Power Input.
3. Heating Box connector.
4. Air Flow Sensor connector.
5. USB port.
6. General Purpose Input Output (GPIO) port.
7. NTC Thermistor port.

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8. Gauge Thermocouple port.
2354 6 7 8
1
Figure 5. Control Unit Overview

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7Installation
The following paragraphs will illustrate how to install and to use the Temperature Control Unit.
H201-T-UNIT-BL. Installation guide
The following instructions shall give you the possibility to install the unit quickly. For safe operation
of the unit it is absolutely necessary to read carefully all the instructions and safety notes.
Electrical Connection
NTC Thermistor
Sensor cable
Heating Box
Power
cord
Fine gauge
thermocouple
8-pins
cables
3-pins
cables
Figure 6. H201-T-UNIT-BL. Standard Connection
1. Connect the Heating Box to the port labeled ‘Heating Box’ on the rear panel of the Temperature Control Unit
(see Figure 6).
2. Plug the NTC Connector on the port labeled ‘Ambient Temperature’ located on the rear panel of the
Temperature Control Unit (see Figure 6). This temperature sensor must be placed in air close to the
illumination pillar in proximity to the microscope stage. This thermistor monitors the air temperature inside
the cage. The NTC thermistor can be also used to monitor the room temperature when working in Sample
Feedback Mode. In this case, you have to place it outside the cage.
3. The Fine Gauge Thermocouple (green) plugs into the green port labeled ‘Reference Temperature’ located on
the rear panel of the Temperature Control Unit (see Figure 6). When operating the device in ‘Sample
Feedback Mode’ place this thermocouple in a Reference Well filled with water. Make sure that the end of the
Fine Gauge Thermocouple is fully immersed.
Tip ►The Fine Gauge Thermocouple may be placed into a Reference Well if running the “Sample Feedback
Mode” (see paragraph 8.2.1.1). The Fine Gauge Thermocouple may also be used to calibrate the Microscope
Incubator temperature if running the “Cage Feedback Mode” (see paragraph 9.1). The Fine Gauge
Thermocouple may be left in air if the system is operated in ‘Cage feedback mode’ (see paragraph 8.2.1.1)
4. Install the microscope enclosure (purchased separately) following the instructions of its dedicated booklet
and reading the paragraph 10 for the positioning of some its components.

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5. Connect the touch screen interface OKO-TOUCH to the Temperature Control Unit using the serial cable
provided (see Figure 7).
OKO-TOUCH
H201-T-UNIT-BL
Figure 7.OKO-TOUCH Connection
6. Connect the 24V-DC Power adapter to the Temperature Control Unit.
7. To switch H201-T-UNIT-BL on use the power button on OKO-TOUCH (see Figure 11). A small green LED light
will blink on the front of the Temperature Control Unit. This means that the system is initializing.
Tip ►Wait until system has reached the steady state before loading your samples.

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How to connect H201-T-UNIT-BL to a BOLD LINE Digital Gas Controller
H201-T-UNIT-BL can be used as a stand-alone device or in combination with any one of the Bold Line Gas
Controllers. Figure 8 shows how to assemble H201-T UNIT-BL, for example, with a CO2-O2 Unit BL [0-10; 1-18].
a. CO2-O2 UNIT-BL [0-10; 1-18] and
H201-T-UNIT-BL. Units rear panels.
b. CO2-O2 UNIT-BL [0-10; 1-18] and
H201-T-UNIT-BL. 3D view.
Figure 8. CO2-O2 UNIT-BL [0-10; 1-18] and H201-T-UNIT-BL
The Gas Controller must stack on top of the Temperature Control Unit by lining up the bus ports located
on the top and bottom surface of each. When the units are properly connected the Temperature, CO2 and O2
parameters will appear on the Touch Screen interface Home page. If not all the parameters appear on OKO-TOUCH
Home Page then the Control Units are not properly connected. Please check that the bus ports are properly aligned
with each other.
If you have the CO2 Controller and a Temperature Control Unit, it is essential to read both
this manual and the Temperature Control Unit Manual to familiarize yourself with the
functions and the operation of the devices before use.

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8User Interface
This chapter describes the user interface of the H201-T-UNIT-BL.
Home page
124
3
5
8
9
10
7
6
Figure 9. Homepage of the H201-T-UNIT-BL Controller Touch Screen Display
1. Status LED
2. Temperature set point (see paragraph 8.1.1 to learn how to change the set point value)
3. Temperature current value
4. Minimun and Maximum Temperature reached within the time frame set in the Options subpage
(see paragraph 8.2.2.1)
5. Chart history length set at 1 hour
6. Display mode. Touch here to switch display mode
7. Overview page
8. Settings. Press here to access system options and settings
9. Home. To open the homepage
10. Product info. Press here to know generic info about OKO TOUCH and H201-T-UNIT-BL and
running time
Tip ►The Touch Screen is pre-set at the following temperature: 37°C. Once turning the Touch Screen on it
will start operating to reach this set-point value.
8.1.1 How to enter the set point temperature
To input a new set-point temperature, touch the corresponding tab, as indicated in Figure 10 a.

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1
2
ab
Figure 10. How to change the Temperature set point (a –b)
The set point regulation page will appear as in Figure 10 b. You can modify the set point by clicking on +
and -. Once you have input the new set point temperature, press “Set” to save or “Cancel” to undo.
Tip ►After any change in the set point value the controller enters into a transient regime, the Status
Indicator and the Top LED turn yellow (see paragraph 8.1.2). During the transient regime, the controller will
not trigger on alarm.
8.1.2 Controller Status: colours led and meaning
The H201-T-UNIT-BL can assume four different status, which are represented by the colors assumed by
the Status Indicator (pointer 1 in Figure 9) and by the TOP LED (if enabled, as indicated in paragraph 8.2.2.3):
The GREEN color indicates that the set-point value has been reached (within the
tolerance defined in the alarm subpage, see paragraph 8.2.2.5) and that the system is working
properly.
Controller Status: NORMAL
The YELLOW color indicates that the controller is in transient regime. The Yellow light
will appear after the controller is turned on and after any set point chance. The system is working
properly, it is not in alarm and no action is needed. As soon as the H201-T-UNIT-BL stabilizes the
temperature, the Controller Status changes to NORMAL and the color turn GREEN. If the H201-
T-UNIT-BL cannot stabilize the temperature of one or both heating devices within the maximum
time defined by the operator (see paragraph 8.2.2.5), the Controller Status changes to ALARM
and the color turn ORANGE.
Controller Status: TRANSIENT
The ORANGE colour indicates that the current Temperature value is out of the tolerance
defined in the alarm subpage (see paragraph 8.2.2.5). The controller triggers in Alarm.
Controller Status: ALARM
The RED color indicates that there is a problem with the unit itself (for example a
Temperature sensor is broken). The system is in alarm. Turn the system off, wait for 5 minutes,
and turn it back on. If the color is still red, contact Okolab at www.oko-lab.com for support.
Controller Status: ALARM
Tip ► The Top LED (see Figure 11) follows the same color code as the status LED.
To activate the Top LED, follow the instructions reported in paragraph 8.2.2.3.

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Top LED
Power Button
Figure 11. Top LED and Power Button.
Settings
8.2.1 Temperature
To choose and insert the proper operation parameters, follow the instruction below:
1. Press on the Settings icon (see Figure 12 a) to enter the Settings menu.
2. Press on the Temperature icon to enter the temperature settings menu, as shown in the Figure 12 b.
ab
Figure 12. Temperature Settings (a –b)
The Temperature settings menu has 5 items, as shown in Figure 13.
Figure 13. Temperature Settings submenu

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8.2.1.1 Control Mode
The Temperature Control can run in two different modes: Sample or Cage Feedback Mode. Press on
Control Mode icon (see Figure 14 a) to choose which one you want to use, Sample or Cage Feedback
Mode. Figure 14 b shows how to select Cage Feedback Mode.
ab
Figure 14. Chamber Mode Settings (a - b)
In this configuration the temperature of the air inside the cage is controlled, precisely in the position
where you have placed the NTC thermistor. It is strongly recommended to attach the NTC thermistor close to the
sample chamber, at right on the stage or on the top condenser (use a standard adhesive tape to fix the sensor on
those parts). The advantage of this solution is that you don’t have to manually stick the fine gauge thermocouple
into a reference well. Once selected the CAGE feedback mode, the operator should calibrate the difference of
temperature between the air inside the enclosure (where the NTC thermistor has been placed) and the specimen
temperature (by sticking the fine gauge thermocouple into a reference well inside the Chamber Incubator), so to
find for instance that to keep the specimen temperature at 37°C, the cage temperature has to be at 38°C (see
paragraphs 8.2.1.5 and chapter 9). This means that when regulating the set-point, given that in this mode you
control the Cage’s temperature, this has to be set at 38°C to keep the specimen at 37°C. This control mode is well
suited for multi-user tasks where users don’t want to take care to correctly position the fine gauge thermocouple
into a reference well inside the chamber incubator; however, it is based on a calibration between cage and
specimen, therefore cannot be considered an active thermal control on the sample
Tip ►Advantages of this Control Mode are: fast experiment start up and no specific action required in multi
user applications. The disadvantage is that it needs calibration between cage and specimen. The temperature
difference between the cage and the sample sensors depends on many factors like: the ambient temperature,
the enclosure design, the sensors position, but if these ones don’t change, the temperature difference doesn’t
change.
Figure 15 b shows how to select Sample Feedback Mode.

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ab
Figure 15. Sample Mode Settings (a - b)
In this configuration, every time you run an experiment, you must place the Fine Gauge Thermocouple
(thin green wire) into a Petri dish or well adjacent to your sample (from here on this will be referred to as
Reference Well). Fill the Reference Well with distilled water to a level allowing full immersion of the fine gauge
thermocouple (see Figure 16). When possible close the petri with its own plastic lid (or with SENSOR LID, see Tip
below) and place it in the appropriate holder. Using a Reference Well in close proximity to your Experimental Well
provides active Sample Feedback Temperature Control. This ensures that your sample will be within 0.1°C from
the temperature set point regardless from room temperature variations.
Figure 16. Free Sensor attached on the internal base of the Petri Dish
Tip ►It is recommended using an Okolab SENSOR LID (available for purchase) to secure the Fine Gauge
Thermocouple in place in the Reference Well during calibration or operation in Sample Feedback Mode (see
Figure 17).
Sensor lids are available for the following: 35 mm, 60 mm Petri-dish, chamber slide and chambered cover
glass. Sensor Lids are ordered separately using the codes in following table.
SENSOR LID CODES
SENSOR LID-35
Temperature sensor lid. To be used in specimen feedback in a 35 mm Petri Dish
SENSOR LID-60
Temperature sensor lid. To be used in specimen feedback in a 60 mm Petri Dish
SENSOR LID-GS
Temperature sensor lid. To be used in specimen feedback in chamber slides and chambered
cover glass
Table 1. Sensors lid codes

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Figure 17. SENSOR LID-35. For 35 mm Petri-dish
Tip ►Advantages of Sample Feedback Control Mode are: direct monitoring and active control of sample
temperature and independence from room temperature variations. The disadvantage is that it requires
manual operation for correct placement of the fine gauge thermocouple in a Reference Well.
8.2.1.2 Fan Speed
The Heating Box has an internal flow sensor that allows regulating the speed of the Air being blown inside
the enclosure. An increased speed allows a quicker start-up and a more uniform thermal distribution inside the
enclosure, a low speed allows instead a smooth operation in applications particularly sensitive to vibrations.
You can set the Air Flow value by pressing on Fan Speed icon (see Figure 18 a). Then you can change
the value in the range 35-100 m3/h (see Figure 18 b). Only when you use the H201-FILTER-BOX, the working range
of the Air Flow becomes 35-70 m3/h, see paragraph 8.2.1.3.
ab
Figure 18. Fan speed setting (a –b).
You can also activate the ‘Fast Heating’ button (see Figure 19), which enables a quick start-up every time
you turn on the system. The ‘Fast Heating’ accelerates the transient time.
SENSOR LID
Fine Gauge
Thermocouple

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Figure 19. Fast Heating Activation.
8.2.1.3 Air filters
If you have purchased also H201-T-FILTER-BOX, a module containing a filters kit composed by a prefilter
and a HEPA filter (High Efficiency Particulate Air Filter) has been added to the Heating Box, see Figure 20. In this
case, Okolab recommends activating the ‘Filter Monitoring’ in the Air Filters subpage.
Press on Air Filters icon (see Figure 21 a), then active “Filter Monitoring” (see Figure 21 b).
H201-T-FILTER-BOX
Figure 20. Heating Box with H201-T-FILTER-BOX
ab
Figure 21. Filter Monitoring activation (a –b).