Okolab H401-T-PENNY User manual

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H401-T-PENNY
Temperature Controller
Manual
IST307_REV02
SV 1.2.6.0

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Index
1PREFACE .....................................................................................................................................................................................3
2SYMBOL DESCRIPTION ..........................................................................................................................................................4
2.1 SYMBOLS USED IN THIS MANUAL...............................................................................................................................................................4
2.2 SYMBOLS ON THE PRODUCT LABEL ...........................................................................................................................................................4
3SAFETY NOTES..........................................................................................................................................................................5
4SUPPLIED EQUIPMENT..........................................................................................................................................................6
5EQUIPMENT DESCRIPTION ..................................................................................................................................................7
5.1 NAVIGATION BUTTONS................................................................................................................................................................................7
5.1.1 ON/OFF device ................................................................................................................................................................................8
6INSTALLATION .........................................................................................................................................................................9
6.1 COMPATIBILITY............................................................................................................................................................................................9
6.2 CONNECTIONS OF THE HEATING DEVICE...............................................................................................................................................10
7USER INTERFACE .................................................................................................................................................................. 11
7.1 HOMEPAGE.................................................................................................................................................................................................11
7.1.1 Status icons ....................................................................................................................................................................................12
7.1.2 Entering the Setpoint temperature.....................................................................................................................................12
7.2 MENU NAVIGATION...................................................................................................................................................................................13
7.2.1 Device selection ...........................................................................................................................................................................14
7.2.1.1 Setting the proper Device Type..................................................................................................................................................................14
7.2.1.2 Advanced: How to manually set the control parameters...............................................................................................................16
7.2.2 Calibration......................................................................................................................................................................................17
7.2.3 Alarm ................................................................................................................................................................................................21
7.2.4 Unit ....................................................................................................................................................................................................22
7.2.5 Status page .....................................................................................................................................................................................22
7.2.6 System info.....................................................................................................................................................................................23
8CLEANING & MAINTENANCE............................................................................................................................................. 24
9SUPPORT ................................................................................................................................................................................. 25
10 TECHNICAL SPECIFICATIONS ....................................................................................................................................... 26
11 TROUBLESHOOTING........................................................................................................................................................ 27
12 FIGURE LIST........................................................................................................................................................................ 28
13 TABLE LIST ......................................................................................................................................................................... 28
14 MANUAL REVISION TABLE ............................................................................................................................................ 28
15 QUICK GUIDE...................................................................................................................................................................... 29

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1Preface
H401-T-PENNY is a compact single-channel temperature controller allowing to set and control the
temperature of a single channel Okolab heating device. It is a reliable solution to maintain the effective desired
temperature of the specimen right on the microscope stage within the range 25-60°C.
H401-T-PENNY includes the Temperature Controller, only. The heating device must be ordered
separately.
A variety of heating devices can be connected to H401-T-PENNY, for example: heating plates, both glass
and metal, for inverted, upright and stereo microscopes; glass heating tables; metal heating pads and objective
heaters. The heating plates –both glass and metal- are designed to fit into the opening of XY stages of upright and
inverted microscopes. Several models of glass and metal heating plates can be selected from the Okolab portfolio.
Custom solutions are available upon request. The glass heating table can be placed in the optical path of any
stereomicroscope and provides a large, flat, clear, warm surface where one or more specimens can be
maintained at physiological temperature. The glass heating table is also suitable for small animal surgery. The
metal heating pads are temperature-controlled surfaces that can be positioned on any laboratory workbench or
desk surface (e.g. table top, cabinet work surface, etc.) to keep Petri dishes, slides, and tubes at the right
temperature before microscope observation. The objective heater is a flexible heated band designed to maintain
the desired temperature of the objective body and lenses. It is necessary when the objective is in contact with the
sample during water dipping or oil immersion observation.
The H401-T-PENNY allows you to perform the heating device calibration by comparing its temperature
value with the value read by an external temperature meter (NOT SUPPLIED).
The H401-T-PENNY features an intuitive interface that combines performance and simplicity.
Okolab recommends reading carefully this manual to familiarize yourself with the functions and the
operation of H401-T-PENNY before use.

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2Symbol description
This paragraph describes the symbols used in this manual and on the product label.
2.1 Symbols used in this manual
The following symbols identify important information to note:
CAUTION or WARNING: this symbol warns you about the risk of electrical shock.
CAUTION or WARNING or IMPORTANT: this symbol warns you of circumstances or practices that
can affect the functionality of the instrument.
Tip ►Supplies you with helpful suggestions.
Note ►Supplies you with important information to successfully use the instrument.
2.2 Symbols on the product label
CE MARKING: this symbol indicates product compliance with EU legislation.
PRODUCT DISPOSAL: this symbol indicates that this product must not be disposed as urban
solid waste.
This symbol indicates the product production date.
This symbol indicates the Manufacturer data.
This symbol indicates the protection degree against ingress of solids or liquids inside the
product.
This symbol warns you to read the user manual before starting the device.
IP 30

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3Safety Notes
In order to achieve best performance and to ensure proper operation of your new equipment, please
read carefully the following safety notes and instructions. If you have any question, please contact Okolab at:
[email protected]
−The equipment must only be used as intended and as described in this Manual.
−Equipment should be operated only by technically qualified personnel.
−Do not start up the equipment if some of its parts are damaged.
−This instrument is not intended for use in locations subject to flammable or explosive gases.
−Transport the equipment with care.
−Equipment and its internal parts can be damaged by dropping and by shock.
−Some equipment parts may reach temperatures above 60°C. Take care when touching it.
−Avoid rapid changes in ambient temperature which may cause condensation, avoid direct air
draft from air conditioner, exposure to direct sunlight and excessive heat accumulation.
−Do not disassemble any part of the system.
−Do not disconnect cables while in operation.
−Do not use a volatile solvent such as paint thinner to clean the instrument, because deformation
or discoloration may occur.
−Use a soft, dry cloth to remove stains from the instrument.
−Do not exceed voltage indicated in this manual and on the product label.
−Avoid excessive induction noise, static electricity and magnetic fields.
−Do not expose this instrument to rain or moisture.
−Prevent throttling and kinking of cables.
−Do not start up the equipment if the supply cable is damaged.
−Connect the equipment only to grounded mains power socket.
−Before starting, assemble the equipment while unplugged from an outlet.
−Prevent metal fragments or lead wire scraps from falling inside instrument case to avoid
electric shock, fire or malfunction.
International caution symbol marks this device. IMPORTANT: read the “Safety Notes” before
installing, using and commissioning this device, as the notes contain important information relating
to safety and EMC. Not following these instructions can result in damage or breakdown of the
device and of its accessories.
We reserve the right to make technical modifications.
IN NO EVENT OKOLAB S.R.L. SHALL BE LIABLE FOR ANY DIRECT, INCIDENTAL OR CONSEQUENTIAL
DAMAGES OF ANY NATURE, OR FINANCIAL LOSS RESULTING FROM IMPROPER USE OF THE PRODUCT.

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4Supplied equipment
1Controller. One channel temperature controller. See Figure 1 (1).
2Device support/cradle. See Figure 1 (2)
3Power supply and power cord. 24V DC-60 W power supply. See Figure 1 (3)
4Heating device connector. See Figure 1 (4)
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4
2
Figure 1. H401-T-PENNY –Components.

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5Equipment Description
H401-T-PENNY is a compact single-channel temperature controller allowing to set and control the
temperature of a single channel Okolab heating device. It is a reliable solution to maintain the effective desired
temperature of the specimen right on the microscope stage within the range 25-60°C.
A detailed description of H401-T-PENNY is provided in the following list, which refers to the pointers in
Figure 2:
1Heating Device connector
2DOWN button (-)
3Display
4UP button (+)
524 VDC Power Input
1 5
234
Figure 2. H401-T-PENNY –Equipment description.
5.1 Navigation buttons
H401-T-PENNY has two navigation buttons:
•DOWN button (see Figure 2 (2))
•UP button (see Figure 2 (4))
There are two different press modes:
1for 1 second
2for 2 seconds

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Keeping the buttons pressed for 1 or for 2 seconds allows you to access different features. The following
table lists the navigation buttons functions.
UP BUTTON for 1 second
Increase Value (+)
UP BUTTON for 2 seconds
Turn OFF/ON
DOWN BUTTON for 1
second
Decrease Value (-)
DOWN BUTTON for 2
seconds
Go to Menu
UP & DOWN BUTTONS at
the same time for 1 second
Enter
UP & DOWN BUTTONS at
the same time for 2 seconds
Cancel/Back
Table 1. UP and DOWN buttons functions.
5.1.1 ON/OFF device
To turn H401-T-PENNY on, keep the UP button pressed for 2 seconds (see Figure 3). The device will turn
ON.
Figure 3. Turning H401-T-PENNY on.
To turn H401-T-PENNY off, keep the UP button pressed for 2 seconds (see Figure 4 a), then press UP for
1 second, following the display info as shown in Figure 4 b. Press DOWN button for 1 second to undo.
ab
Figure 4. Turning H401-T-PENNY off (a –b).

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6Installation
H401-T-PENNY can control the temperature of a single heating device.
6.1 Compatibility
H401-T-PENNY is compatible with the following Okolab heating devices:
−H601-T-GLASS series*
−H401-T-METAL series
−H401-T-PAD series
−H401-GLASS-TABLE
−OBJ-COLLAR-SERIES
*single channel
For a complete list of compatible heating devices, refer to: www.oko-lab.com.

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6.2 Connections of the heating device
Do not turn H401-T-PENNY ON until you have completed and checked all connections.
Follow the instructions below to connect the heating device to H401-T-PENNY.
24 VDC
Power
connector
Heating
Device
connector
Figure 5. Connection of the heating device.
1Connect the heating device to the Heating Device connector of H401-T-PENNY (see Figure 5 and Figure 2
(1)).
2Connect the Power Supply to the 24 VDC Power connector (see Figure 5 and Figure 2 (5)).
Use only the power supply provided by Okolab, to prevent system instability, random
reset, unexpected shutdown, or problems due to insufficient power.
For assistance, contact Okolab at [e-mail].
Do not connect AC power supply to your device until you have completed all the
connections shown in Figure 5.

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7User Interface
This chapter describes the user interface of H401-T-PENNY.
7.1 Homepage
Figure 6 shows the main display, in particular it highlights 5 points illustrated below.
1 2
4
3
5
Figure 6. Home page.
1Device type category: it is the type of heating device connected to H401-T-PENNY. See paragraph 7.2.1.1
2Status Icon: it represents the device status. See paragraph 7.1.1.
3Device Minimum and Maximum temperatures reading within 1 minute.
4Device temperature Setpoint. See paragraph 7.1.2.
5Device temperature current value.
Tip ►Setpoint Temperature is pre-set at 37. Once turned on, H401-T-PENNY will start operating to reach
37.
Note ►If no device is connected, the display shows OPEN, see Figure 7.
Figure 7. Home page with no device connected.

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7.1.1 Status icons
H401-T-PENNY can assume four different Status, represented by four Status Icons (see Figure 6 (2)).
The Check Mark icon indicates that the Setpoint value has been reached (within the
tolerance defined in the alarm subpage, see paragraph 7.2.3) and that the system is working
properly.
Controller Status: NORMAL
The Hourglass icon indicates that the controller is in transient regime. The Hourglass icon
will appear immediately after the controller is turned on and after any Setpoint change. The
system is working properly, it is not in alarm and no action is needed. As soon as H401-T-PENNY
stabilizes the temperature of the heating device, the Controller Status changes to NORMAL and
the Check Mark icon appears. If the H401-T-PENNY cannot stabilize the device temperature
within the maximum time defined by the operator (see paragraph 7.2.3), the Controller Status
changes to ALARM and the Alert icon appears.
Controller Status: TRANSIENT
The Alert icon indicates that the current Temperature value is out of the tolerance
defined in the alarm subpage (see paragraph 7.2.3).
Controller Status: ALARM
The Error Cross icon indicates that there is a problem with the unit itself (for example a
Temperature sensor is broken or the Heating device is disconnected). The system is in alarm.
Turn the system off, wait for 5 minutes, and turn it back on. If the icon is still present, contact
Okolab at [e-mail] for assistance.
Controller Status: ALARM
7.1.2 Entering the Setpoint temperature
To input a new Setpoint temperature of the heating device, follow the instructions below:
1. Press the UP or DOWN button for 1 second (as indicated in Figure 8 a) and the Setpoint will highlight (see
Figure 8 b).
ab
Figure 8. Definition of the Setpoint (a –b).
2. Press again the UP or DOWN button to increase (as indicated in Figure 9) or decrease the Setpoint value.
Tip ►By keeping pressed the UP or DOWN button for 2 seconds, the scrolling becomes faster.

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Figure 9. Input of the Setpoint value.
3. Confirm the Setpoint value by pressing UP & DOWN buttons at the same time for 1 second (see Figure 10 a).
To cancel and return to the old Setpoint, press UP & DOWN buttons at the same time for 2 seconds or
wait 5 seconds without pressing any button.
ba
Figure 10. Confirm the Setpoint value (a –b).
Note ►After any change in Setpoint value the controller enters into a transient regime. The Alert icon
will appear, as in Figure 10 b. During the transient regime, the controller will not go in alarm. As soon as
H401-T-PENNY stabilizes the temperature of the heating device, the controller status changes to NORMAL
and the Check Mark icon appears.
7.2 Menu navigation
Press DOWN button for 2 seconds to access the Menu page (see Figure 11). Press UP or DOWN button for
1 second to scroll the menu items (see Figure 12) and choose the submenu. Submenus are: Device selection,
Calibration, Alarm, Unit, Status, System info.

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Figure 11. How to access to Menu navigation: press DOWN button for 2 seconds.
ab
Figure 12. H401-T-PENNY Menu. Scroll by pressing UP or DOWN button for 1 second a) page1/2; b) page 2/2.
7.2.1 Device selection
The Device selection menu allows to select the type of heating device connected to H401-T-PENNY.
7.2.1.1 Setting the proper Device Type
To set the proper Device Type follow the instructions below:
1. Read the Type Number from the Product Label (see Figure 14).
2. Press UP & DOWN buttons at the same time for 1 second to enter the Device selection submenu (see Figure
13 a).
3. Use UP or DOWN button to scroll the Type number list (see Figure 13 b).
Tip ►By keeping pressed UP or DOWN button for 2 seconds, the scrolling becomes faster.

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ba
Figure 13. Device selection (a) and Device selection submenu (b).
4. When the Type Number shown on the display corresponds to the Type Number on your heating device label
(see Figure 14), press UP & DOWN buttons at the same time for 1 second to select it (see Figure 15). To
cancel and return to menu page press UP & DOWN buttons at the same time for 2 seconds or wait 5 seconds
without pushing any button.
DEVICE TYPE
NUMBER
Figure 14. Heating device label.
Figure 15. Selecting the Device Type Number.
H401-T-PENNY is pre-set with Type 010. Once turned on, it will start operating with
the parameters of Device Type 010. To optimize the temperature control of your
heating device, set the Type Number that you can read on the heating device label.

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7.2.1.2 Advanced: How to manually set the control parameters
You can manually input the control PID parameters by selecting “Type 000” and inserting the values for
K1, T1, K2, T2, Delta T parameters.
Tip ►When selecting “Type 000”, the display shows “Custom” as Device Type. See Figure 16.
Figure 16. Device Type 000 allows manual setting of control parameters.
Follow the instructions below to modify control PID parameters:
1. Press UP & DOWN buttons at same time for 1 second (see Figure 17 a) to enter the list of control parameters
(see Figure 17 b).
2. Scroll by pressing UP or DOWN button for 1 second.
3. Press UP & DOWN buttons at same time for 1 second to enter K1 subpage (see Figure 17 c).
4. Use UP button to increase (or DOWN button to decrease) the K1 value (as shown in Figure 17 d).
5. Press UP & DOWN buttons at the same time for 1 second to confirm (see Figure 17 e).
6. Repeat steps 1,2,3, 4 and 5 to insert values for T1, K2, T2 and Delta T parameters.
To know the correct parameters values for you device or to receive more information about PID
parameters contact Okolab at [e-mail].

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ba
cd
e
Figure 17. Custom Device Type 000: how to set the control parameters (a –b –c –d - e).
7.2.2 Calibration
H401-T-PENNY allows to calibrate the connected Heating Device. To do so, you need an accurate
independent Temperature Sensor with which to measure the temperature of the liquid inside the sample holder.
The Calibration is completed when you achieve the desired temperature of the liquid inside the sample holder.
The outcome of the calibration is the offset value between the temperature of the liquid inside the sample holder
and the temperature of the Heating Device.
Tip►You can use LEO as external meter to make an accurate calibration.
To start the calibration, follow the instructions below:
1. Secure the independent T Sensor to the bottom of the sample holder (Petri dish, Chambered Glass Slide, etc.)
with commercial adhesive tape as shown in Figure 18. Do not cover the tip of the T-Sensor.
Tip►Use the same sample holder that you use in regular practice.

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Figure 18. T Sensor secured inside a Petri Dish
2. Fill the sample holder with oil or water to the level used during regular practice. Make sure the tip of the
independent T Sensor is immersed in the liquid, inside the sample holder (see Figure 19).
Figure 19. Fill the sample holder with oil
3. Cover the sample holder with the lid or leave it open, according to the protocol followed during regular
practice.
4. Place the petri-dish on the center of the heating device, as shown in Figure 20.

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H401-T-PENNY
(LEO) NOT SUPPLIED
PETRI
Temperature sensor
HEATING DEVICE
Figure 20. H401-T-PENNY: manual calibration with LEO
5. Wait until the temperature measured by LEO (or by any other independent sensor) is stable.
6. Make sure that H401-T-PENNY has reached the steady state, i.e. the status indicator is the Check Mark icon
(see paragraph 7.1.1).
7. Press DOWN button for 2 seconds to access the Menu page (see Figure 21 a) and use the DOWN button to
select the Calibration page (see Figure 21 b).
ba
Figure 21. How to enter the Calibration Submenu.
8. Press UP & DOWN buttons at the same time for 1 second to enter the Calibration page (see Figure 22 a), the
page shown in Figure 22 b will appear.
Tip ►In the Calibration page you can read the Offset value, the Device Temperature current value (T) and
the Device Temperature Setpoint (SP).

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ba
Figure 22. Calibration. (a) How to enter the calibration menu (b) Calibration menu.
9. Insert the offset value by pressing UP or DOWN button, as shown in Figure 23 a and press UP & DOWN
buttons at the same time for 1 second to save (see Figure 23 b).
Tip ►Okolab recommends introducing as offset value the difference between the actual temperature
measured by the independent T Sensor and the Setpoint temperature. In the example shown in Figure 20,
LEO reports 37.2°C and H401-T-PENNY reads 37°C, the Offset value will be 0.2°C, as shown in Figure 23.
Note ►After changing the offset, it is advisable to wait at least 10-20 minutes to ensure that the
temperature inside the sample holder is stable.
ba
Figure 23. Insert the Device Offset value (a - b).
10. If the temperature measured by the independent T Sensor is within the ±0.3°C from Setpoint, you can
consider the Manual Calibration as completed. Otherwise, repeat the steps 7 to 9 until the temperature
measured by the independent T Sensor reaches the Setpoint temperature within ±0.3°C.
11. Once the manual calibration is completed you can remove the independent T Sensor from the petri dish and
start using the calibrated Heating Device.
If room temperature changes by more than 1°C during the calibration time or if air draft blows
directly on the heating device you will not be able to achieve an accuracy in the liquid of ±0.3°C.