Opentrons OT-2 User manual

OT-2 Liquid Handling Robot
Quickstart Guide
Opentrons Labworks Inc.
Edition
Revision OT-2R, April 2022

Post-sales service & contacting Opentrons
If you have any questions about the use of the system, abnormal
phenomena, or special needs, please contact:
[email protected]. Also visit www.opentrons.com.

– Included Box Contents / Parts & Accessories
– Machine Setup
Table of Contents
Product and Manufacturer Description 4
Product Elements 5
Instructions for Unboxing and Use 7
Instructions 11
Online Resources 22
Cleaning and Maintenance 22
Support 22
Specs 23

OT-2 MANUAL
Product and Manufacturer
Description
PRODUCT DESCRIPTION
The OT-2 is a liquid handling lab robot. It is open-source, highly
customizable, kit- and reagent-agnostic, and takes up half a lab bench.
Its software and hardware modules are built by scientists for scientists
and don’t require coding knowledge to use.
Completed Certications
CE & FCC, NRTL, CB, & ISO 9001
Not Certied/Validated
IVD & GMP
MANUFACTURER DESCRIPTION
Opentrons Labworks Inc
45-18 Ct Square W
Long Island City, NY 11101

5
Product Elements
Serial Number
The OT-2 serial number indicates its date
of manufacture, in the following format:
OT2XXXYYYYMMDDXXX
OT-2
66CM/26IN
57CM/22.5IN 63CM/25IN
0.1mm resolution
xyz gantry
Polycarbonate
enclosure
Easily removable deck
2 interchangeable
pipette heads

OT-2 MANUAL
Product Elements
DECK OUTLINE
11 deck slots
Adapters allow for existing
hardware to t on deck
Deck slots are compatible with standard SBS
dimensions. Deck also includes a removable trash bin.
Modules snap into place

7
Instructions for
Unboxing and Use
To get started quickly, visit our online resources and follow the
step by step instructions to help you go from unboxing your
robot to running your rst protocol.
Visit https://support.opentrons.com/ for more information.
INCLUDED BOX CONTENTS / PARTS & ACCESSORIES
◾(1) OT-2
◾(3) Window Covers
◾(2) Side Window Covers
◾(1) Rear Window Cover
◾(1) Power Supply (36V/6A)
◾(1) Regional IEC Power Cable
◾(1) Ethernet Cable
◾(1) Ethernet-to-USB Dongle (connects the OT-2 to a laptop)
◾(1) Toolkit
◾Hand Screwdriver (2.5mm)
◾14mm Wrench
◾Torx Key (T10)
◾Hex Key Set (1.5mm, 2mm, 2.5mm, 3mm)
◾Super Lube (silicone-based lubricant)
◾(2) M3 Hex Nut
◾(2) M4 Square Nut
◾Window Screws
◾(4) M4x12mm low prole socket screw, M4 thick washer,
and rubber O-ring
◾Pipette Screws
◾(4) M3x6mm Socket Head Screws (2 are spares)
◾(1) Trash Bin
◾(1) Calibration Block
◾(1) Top Window

OT-2 MANUAL
Instructions for
Unboxing and Use
(1) OT-2
(3) Window Covers
(2 side window covers,
1 rear window cover)
(1) Power Supply (36V/6A)
(1) Ethernet Cable
(1) Ethernet-to-USB dongle
(Connects the OT-2 to a
laptop)
(1) 14mm Wrench
(1) Hand Screwdriver
(2.5mm)
(1) Regional IEC Power Cable

9
(1) Torx Key (T10)
(1) Super Lube
(silicone-based lubricant)
(4) Pipette Screws
(M3x6mm socket head
screws, 2 are spares)
(1) Hex Key Set
(1.5mm, 2mm,
2.5mm, 3mm)
(2) M3 Hex Nut
(2) M4 Square Nut
(4) Window Screws
(M4x12mm low prole socket screw,
M4 thick washer, and rubber O-ring)
(1) Calibration Block
(1) Trash Bin

OT-2 MANUAL
REQUIRED FOR OPERATION
◾A computer (self-provided)
◾An open-end wrench (included)
◾Space larger than 625mm x 570mm x 680mm