Opentrons OT-2 User manual

OT-2 Liquid Handling Robot
Quickstart Guide
Opentrons Labworks Inc.
Edition
Revision OT-2R, April 2022

Post-sales service & contacting Opentrons
If you have any questions about the use of the system, abnormal
phenomena, or special needs, please contact:
[email protected]. Also visit www.opentrons.com.

– Included Box Contents / Parts & Accessories
– Machine Setup
Table of Contents
Product and Manufacturer Description 4
Product Elements 5
Instructions for Unboxing and Use 7
Instructions 11
Online Resources 22
Cleaning and Maintenance 22
Support 22
Specs 23

OT-2 MANUAL
Product and Manufacturer
Description
PRODUCT DESCRIPTION
The OT-2 is a liquid handling lab robot. It is open-source, highly
customizable, kit- and reagent-agnostic, and takes up half a lab bench.
Its software and hardware modules are built by scientists for scientists
and don’t require coding knowledge to use.
Completed Certications
CE & FCC, NRTL, CB, & ISO 9001
Not Certied/Validated
IVD & GMP
MANUFACTURER DESCRIPTION
Opentrons Labworks Inc
45-18 Ct Square W
Long Island City, NY 11101

5
Product Elements
Serial Number
The OT-2 serial number indicates its date
of manufacture, in the following format:
OT2XXXYYYYMMDDXXX
OT-2
66CM/26IN
57CM/22.5IN 63CM/25IN
0.1mm resolution
xyz gantry
Polycarbonate
enclosure
Easily removable deck
2 interchangeable
pipette heads

OT-2 MANUAL
Product Elements
DECK OUTLINE
11 deck slots
Adapters allow for existing
hardware to t on deck
Deck slots are compatible with standard SBS
dimensions. Deck also includes a removable trash bin.
Modules snap into place

7
Instructions for
Unboxing and Use
To get started quickly, visit our online resources and follow the
step by step instructions to help you go from unboxing your
robot to running your rst protocol.
Visit https://support.opentrons.com/ for more information.
INCLUDED BOX CONTENTS / PARTS & ACCESSORIES
◾(1) OT-2
◾(3) Window Covers
◾(2) Side Window Covers
◾(1) Rear Window Cover
◾(1) Power Supply (36V/6A)
◾(1) Regional IEC Power Cable
◾(1) Ethernet Cable
◾(1) Ethernet-to-USB Dongle (connects the OT-2 to a laptop)
◾(1) Toolkit
◾Hand Screwdriver (2.5mm)
◾14mm Wrench
◾Torx Key (T10)
◾Hex Key Set (1.5mm, 2mm, 2.5mm, 3mm)
◾Super Lube (silicone-based lubricant)
◾(2) M3 Hex Nut
◾(2) M4 Square Nut
◾Window Screws
◾(4) M4x12mm low prole socket screw, M4 thick washer,
and rubber O-ring
◾Pipette Screws
◾(4) M3x6mm Socket Head Screws (2 are spares)
◾(1) Trash Bin
◾(1) Calibration Block
◾(1) Top Window

OT-2 MANUAL
Instructions for
Unboxing and Use
(1) OT-2
(3) Window Covers
(2 side window covers,
1 rear window cover)
(1) Power Supply (36V/6A)
(1) Ethernet Cable
(1) Ethernet-to-USB dongle
(Connects the OT-2 to a
laptop)
(1) 14mm Wrench
(1) Hand Screwdriver
(2.5mm)
(1) Regional IEC Power Cable

9
(1) Torx Key (T10)
(1) Super Lube
(silicone-based lubricant)
(4) Pipette Screws
(M3x6mm socket head
screws, 2 are spares)
(1) Hex Key Set
(1.5mm, 2mm,
2.5mm, 3mm)
(2) M3 Hex Nut
(2) M4 Square Nut
(4) Window Screws
(M4x12mm low prole socket screw,
M4 thick washer, and rubber O-ring)
(1) Calibration Block
(1) Trash Bin

OT-2 MANUAL
REQUIRED FOR OPERATION
◾A computer (self-provided)
◾An open-end wrench (included)
◾Space larger than 625mm x 570mm x 680mm

11
Instructions
MACHINE SETUP
Remove the top foam and window and top covers.
Remove the power cable box from the foam, if present.
1
Carefully cut open the plastic VCI bag. Be sure to avoid
cutting any machine components inside.
2

OT-2 MANUAL
The machine requires two people for lifting out of
the packaging (the machine weighs over 40kg).
Pull upwards using the black steel frame.
3
4
Instructions

13
Cut the zip ties supporting the customer kit and
place the kit aside.
Place the machine on a secure and stable surface.
5
6

OT-2 MANUAL
Pull the remaining zip tie ends and remove completely from
the deck.
8
Remove tape from the calibration switch cover.
8
7
8
Instructions

15
Remove the plastic cover completely.
9
Put the trash bin in place.
10

OT-2 MANUAL
Remove the 3 transport fasteners:
11
1. Unlock and remove the x-axis locking bracket (red) and 4x
locking screws using the 3mm hex key
Instructions
3
2
1
2. Unlock and remove the z-axis locking bracket (red) and
4x locking screws using the 3mm hex key. Note that after
removing the rear screws, one can move the z-stage further
left by hand, allowing more room for removing the screw.
3. Unlock and remove the y-axis locking bracket (red) and
1x locking screw using the 3mm hex key

17
Remove the lm used to protect the polycarbonate sheets.
12
Install the top polycarbonate sheet and fasten the corner
screws using the 2.5mm hex screwdriver and 4x top
window screws.
13
1
2
3 4
5

OT-2 MANUAL
Check the machine:
14
Conrm that 3 brackets have been removed, as powering
on the robot with brackets may cause damage.
Conrm that you are not missing any required cables or
parts. If you are missing components, contact Customer
Support.
Visually check the deck and windows; if there are any
signicantly scratched, broken, or bent components,
contact Customer Support.
Visually check the gantry; if it is uneven or slanted, contact
Customer Support.
Move the pipette head in the x and y direction; if it makes
any loud/abnormal noises or requires a large thrust to
move, contact Customer Support.
Instructions

19
Clean & sterilize robot
16
Note: When cleaning and maintaining the machine, be sure
to turn o the machine and disconnect the power before
proceeding. Also pay attention to warning labels
Install window covers
15

OT-2 MANUAL
Connect power source & USB/Ethernet cable
17
POWER AND CONNECTION
Connect Ethernet and connect the power cable.
If your computer does not have Ethernet use the
Ethernet-to-USB dongle provided.
POWER REQUIREMENTS
100-240 VAC / 50-60 Hz
220 W max
CONNECTIVITY
Wi-Fi 2.4 GHz IEEE 802.11b/g/n, USB 2.0.
Instructions