opti-sciences ccm-200 plus User manual

CCM-200 plus
Chlorophyll Content Meter

Opti-Sciences, Inc. CCM-200 plus operator's manual
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Table of contents
TABLE OF CONTENTSINTRODUCTION ............................................................................................................2
INTRODUCTION.......................................................................................................................................................3
WELCOME!................................................................................................................................................................3
LIST OF EQUIPMENT...................................................................................................................................................3
ABOUT THE INSTRUMENT ..........................................................................................................................................3
APPLICATIONS &FEATURES......................................................................................................................................4
Applications.......................................................................................................................................... 4
Features................................................................................................................................................ 4
How it Works........................................................................................................................................ 5
PHYSICAL FEATURES .................................................................................................................................................6
OPERATION OVERVIEW .......................................................................................................................................6
SYSTEM SETTINGS .....................................................................................................................................................7
MEASUREMENT &OPERATION ..................................................................................................................................7
MEASUREMENTS........................................................................................................................................................8
Setup..................................................................................................................................................... 8
CALIBRATION ............................................................................................................................................................9
TEST TYPES .............................................................................................................................................................10
Single point measurement.................................................................................................................. 10
Multi-Point averaging........................................................................................................................ 10
Multi-Point averaging with std. deviation flyer removal................................................................... 10
ENTER COMMENTS FOR MEASUREMENTS ................................................................................................................12
DATA MANAGEMENT...........................................................................................................................................14
TRANSFER DATA BY USB ........................................................................................................................................14
SENDING DATA BY RS232. ......................................................................................................................................14
Communication Settings.................................................................................................................... 15
TECHNICAL SPECIFICATIONS..........................................................................................................................16
ERROR CODES........................................................................................................................................................17
TECHNICAL SUPPORT .........................................................................................................................................22
REFERENCES:.........................................................................................................................................................23

Opti-Sciences, Inc. CCM-200 plus operator's manual
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Introduction
The CCM-200 plus is easy to operate. Contained herein are a few simple guidelines to assist the
user. Throughout this manual, you will be shown setup options and response messages. When an
example of a program screen is shown within a box, this is how it will appear on the CCM-200
plus.
Welcome!
Congratulations on your purchase of the CCM-200 plus Chlorophyll Content Meter. Please
check the carton for any visible external damage. If you notice any damage, notify the freight
carrier immediately. Follow their procedures for reporting and filing a claim. The carton and all
packaging should be retained for inspection by the carrier or their insurer.
List of Equipment
Report any missing items immediately. You should have received the following items:
• CCM-200 plus Chlorophyll Content Meter
• USB cable
• 9V Battery
• CD with manual
About the Instrument
The CCM-200 plus Chlorophyll Content Meter is a hand-held, battery-operated instrument
designed for the rapid, non-destructive, determination of chlorophyll content in intact leaf
samples. Chlorophyll content is a direct indication of plant health and condition. Obtaining
chlorophyll content via non-destructive analysis gives researchers, agronomists and growers
valuable diagnostic information. This data can then be applied to a multitude of crop production
and research initiatives such as nutrient and irrigation management, pest control, environmental
stress evaluation and crop breeding.
Measurements are instantaneous and can be done in the field under normal lighting and growing
conditions. Ample on-board data logging and simple, easy to understand measurements further
enhance the user’s ability to gather and interpret crop health conditions. The USB cable included
with the CCM-200 plus allows for data transfer to a PC. for additional analysis.
The CCM-200 plus is a remarkable alternative to destructive sampling techniques. It is far less
time consuming and allows samples to be monitored multiple times over various stages of an
entire growth cycle. The rapid test and data gathering capability is sure to provide a tremendous
advantage to all types of research. This innovative pocket-sized instrument is destined to play an
important role in improving crop yield and producing higher quality foods.

Opti-Sciences, Inc. CCM-200 plus operator's manual
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Applications & Features
The CCM-200 plus Chlorophyll Content Meter is a versatile tool for Crop, Plant and Agronomic
Sciences. Listed below are a few examples of the many applications for which the CCM-200
plus can be used along with the user-friendly features that make the instrument indispensable to
the field researcher.
Applications
• Optimization of Nitrogen Management
• Detecting & Assessing Crop/Plant Stress
• Nutrient Deficiency Analysis
• Plant/Crop Physiology
• Study Effects of Herbicides/Pesticides
• Monitor Response to Environmental Stress
• Leaf Senescence Studies
• Breeding and Production
• Teaching
Features
• Non-Destructive Measurement
• Lightweight, Hand-Held Design
• Optimized for Field Work
• Battery Operated
• Accurate Reliable, Easily Repeatable Measurements
• Internal Data Logging
• Stand Alone Operation- No PC is Required
• Simple to Use
• Rapid Test & Data Acquisition
• Single reading; averaging of multi-point readings, or averaging of 10 to 30 readings
with standard deviation elimination function, used to throw out readings that are outside
of a two sigma range.
• Includes a port for external GPS. The GPS can be purchased separately. The GPS
allows the recording of measurement values with location information.
• Graphic display of Averaging measurements.

Opti-Sciences, Inc. CCM-200 plus operator's manual
How it Works
Chlorophyll has several distinct optical absorbance characteristics that the CCM-200 plus
exploits in order to determine relative chlorophyll concentration. Strong absorbance bands are
present in the blue and red but not in the green or infrared bands.
Figure 1 chlorophyll absorbance
CCI = %Transmittance at 931nm. / %Transmittance at 653nm.
The CCM-200 plus uses transmittance to estimate the chlorophyll content in leaf tissue. Two
wavelengths are used for absorbance determinations. One wavelength falls within the
chlorophyll absorbance range while the other serves to compensate for mechanical differences
such as tissue thickness (see figure 2). The meter measures the transmittance of both
wavelengths and calculates a CCI (chlorophyll content index) value that is proportional to the
amount of chlorophyll in the sample.
Note: CCI value is a relative chlorophyll value. Absolute chlorophyll content per unit area is not
computed. CCM-200 plus measurements however can be correlated to ground/solvent analysis.
Figure 2. LED Spectrum
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Opti-Sciences, Inc. CCM-200 plus operator's manual
Physical Features
Measuring lever
Display screen
Selection buttons
On - off button
Arrow keys
USB port
GPS port
Operation Overview
The CCM-200 plus is a self-contained, handheld device powered by a 9V alkaline battery. The
sample head is located at the top of the instrument. A liquid crystal display (LCD) is located near
the center of the unit. The power switch is at the bottom of the front panel. Seven menu / control
keys are used to access all program functions.
Program flow is organized into 3 menu groups. The MENU key will step through the selections.
Press ENTER to accept the currently displayed value. Remaining keys are used within each
mode as later described.
A USB port is located at the bottom right hand side of the case and allows the user to export data
to a PC. A RS232 port is included on the bottom for use with GPS, and it can also be used for
data transfer.
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Opti-Sciences, Inc. CCM-200 plus operator's manual
The three green tear shaped buttons are for selecting software defined choices and will be
described as “buttons”. The blue arrow keys are used for scrolling and referred to as “arrow
keys”.
Note: The unit has a 4 minute auto-off feature. When no key is pressed, or test run for 4 minutes
the unit will shut off automatically in order to conserve battery power.
System settings
At the main test menu, choose System
Serial port output
Baud rate [USB]
Measurement & Operation
Described on the following pages are the specifics on how to obtain measurements with the
CCM-200 plus as well as the overall instrument operation protocols. Calibration, measurements,
memory and data storage management and t he five operating modes and each key function are
outlined in detail.
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Opti-Sciences, Inc. CCM-200 plus operator's manual
Measurements
Turn on the instrument. After a few seconds, the main menu screen appears.
Press the Run button.
Before measuring, use setup to select the correct parameters and test type.
Setup
Use the arrow keys to scroll through the options.
When the option of interest is displayed, select the edit button.
A cursor will appear. Use the arrow keys to highlight your selection.
Press the exit button when finished.
File name creation
To create a new file name, select edit from the "CCM200p Test Setup" screen. Use the right
arrow to select the Data File. The File name manager screen will appear. "Use an existing file”
will be highlighted. Use the up/down arrows to scroll through the options until “Enter a new
name” is displayed. Press accept. The new file name screen will appear. Use the up/down arrows
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Opti-Sciences, Inc. CCM-200 plus operator's manual
to scroll through numbers 0-9, and A-Z. To move to the next or previous characters, use the right
and left arrows. Press accept to save the entry. The newly created file name is now set to be used
for measurements. Press exit to return to the Test Menu.
Note: A newly created file name will not appear on the list of existing measuring files until a
measurement has been made.
Using an existing file name
To select an existing measuring file, select edit from the "CCM200p Test Setup" screen. Use the
right arrow to select the Data File. The File name manager screen will appear. "Use an existing
file” will be highlighted. Press Accept. Use the up/down arrows to scroll through the available
file names, and press accept when the one you want is highlighted. The accepted file name is
now set to be used for measurements. Press exit to return to the Test Menu.
Calibration
Calibration is required every time the unit is turned on.
The unit’s detection system needs to check its chamber path. The user has to calibrate or “zero”
the instrument each time the unit is powered up.
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Opti-Sciences, Inc. CCM-200 plus operator's manual
To Calibrate the unit, close the measuring chamber.
Important: make sure chamber is clear of any material.
Hold the chamber closed until the release arm message is displayed on the screen.
Calibration is not required in-between measurements, only when the system is powered up. The
unit is ready to take another measurement once the current measurement is displayed.
Test Types
There are three test types on a standard CCM-200 plus.
Single point measurement
This test saves every data point. Place the sample in the chamber, and press the head closed. A
value will appear when the test is complete. The next measurement may then be taken.
Multi-Point averaging
Multi-point averaging is used for some nitrogen management schemes. One can choose to
average from 2 to 30 measuring points Please refer to the following papers for more details.-
(Shapero 2006) (Francis 1999) The papers listed here, use 30 point averaging and 10 point
averaging respectively. Some crop consultants use fewer points to save time.
Multi-Point averaging with std. deviation flyer removal
This is the same test as listed above, but it will only allow averaging from 10 to 30 samples. If
the difference between measurements is greater than 2 sigma, the instrument automatically
throws out the measurement, and requires an additional measurement.
From the Test Menu and select setup.
Use the arrow keys to scroll through the various parameters available until the Test Type screen
is displayed.
Test Menu
Measure Back Setup
CCM200p Test Setup
Save Data [Yes]
Data File [FileName]
Exit Edit
[ Multi-point average ]
Average ## points[10]
Record point data [Yes]
Exit Edit
[ Multi-point std. dev ]
Average ## points[10]
Record point data [Yes]
Exit Edit
[ 1 point no averaging ]
Average ## points[10]
Record point data [Yes]
Exit Edit
Use vertical blue arrows
Press the edit green button, and se blue arrowsu
Select Edit, and use the arrow keys to scroll through the options until Multi-point average is
highlighted. Press the horizontal blue arrow until the Average ## points is highlighted. Use the
vertical arrows to select the number of points to be averaged. Press the Enter button to accept the
selection.
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