OPTO-EDU A12.1095 User manual

A12.1095
Research Scientific Laboratory Microscope,
Full-Auto Motorized
Instruction Manual
This manual is for users of A12.1095 scientific research microscope. To ensure your safety,
obtain optimum performance, and to familiarize yourself fully with the use of this
microscope, we recommended that you study the instruction manual carefully.

1
Contents A12.1095
User Notices......................................................................................2
1、Components Name...........................................................................4
2、Structure Overview..........................................................................5
3、Adjustment And Operation.............................................................7
3-1、Turn On The Power.......................................................................7
3-2、Adjust The Brightness and Switch Light Source...............................7
3-3、Light Source Socket.......................................................................7
3-4、Mount Specimen............................................................................8
3-5、Adjust Interpupillary Distance........................................................8
3-6、Three Light Path Switching............................................................8
3-7、Focusing........................................................................................9
3-8、Adjusting the condenser…………………………………………10
3-9、Using the frosted filter...…………………………………………11
3-10、Using the extensional function board …………………………11
3-11、Computer control signal interface and photo function key …...……11
3-12、Additional function keys ……………………………………...………12
3-13、Touch screen ………………………………………………..…………12
4、Installation ………………………………………………….………………13
4-1、Installing Diagram ………………………………………..……………13
4-2、Installing Steps………………………………………….………………14
4-2-1、Installing the stage holder……………………….………………14
4-2-2、Installation the electric stage……………………………………14
4-2-3、Installing the nosepiece…………………………….……………15
4-2-4、Installing the viewing head and photo attachment……………15
4-2-5、Installing the eyepiece…………………………………...………15
4-2-6、Installing the Objective……………………………….…………16
4-2-7、Installing the condenser ………………………………...………16
4-2-8、Replacing the camera interface………………………....………17
5、Technical specification…………………………………………...………18
6、Troubleshooting…………………………………………....………………19

2
User Notices
A12.1095
Ⅰ.Safety Symbols
Ⅱ.Safety Notes
1. Open the box carefully to avoid the accessories, like lens, being polluted by fingerprint or
sweat strain, dropping to ground and being damaged.
2. Do keep the instrument out of direct sunlight, high temperature or humidity, dusty and easy
shaking environment. Make sure the stage is smooth, horizontal and firm enough.
3. When moving the microscope, one hand hook into the upper slot behind the frame, and the
other hand hold the front bottom of frame.
4. If the bacterium solution or the water splash to the stage, objective or viewing tube, pull out
the power cord at once, and wipe up the microscope. Otherwise, the instrument will be damaged.
5. Be sure to keep at least 10cm away from the left, right, top, rear of the microscope from walls
and other objects, so as not to plug natural convection air, which is used for cooling.
6. Make sure the instrument is earthed, to avoid lightning strike.
7. Check the input voltage: be sure the input voltage which signed in the back of the microscope
is consistent with the power supply voltage, or it will bring a serious damage to the instrument.
Symbol
Meaning
Main power switch is on
Main power switch is off
/
Upper / Lower lighting
When the light switching button is pushed-in, the light source on the top
will open.When the light switching button is pushed-out, the light on the
bottom will open.
Trash
F.S-Diaphragm, ---Aperture diaphragm opening direction,
---Narrow direction
Use a wrench here
Light intensity direction. Turn toward the tip, the light intensity changes
from strong to weak
Pass CE
Push in the pull rod completely, the brightness ratio is 100:0
Push the pull rod to the middle, and the brightness ratio is 20:80
Pull out the pull rod completely and the brightness ratio is 0:100
When the switch handle of frosted filter is pressed, the frosted filter will
make light path

3
A12.1095
Ⅲ.Maintenance
1. All the lenses have been well checked and adjusted. It is forbidden to disassemble them
yourself.
2. The nosepiece, coarse and fine focusing parts are so delicate that it is forbidden to
disassemble them carelessly by yourself.
3. Keep the instrument clean, wipe dust regularly, and be attention to avoid contaminating the
optical elements especially. The objective lenses should be cleaned every month by professional.
4. We can clean the contaminations such as fingerprints and oil smudges on the prism by using
the tissue paper and solution, which is mixed by ether(70%) and alcohol(30%).
Noting that alcohol and ether are highly flammable, do keep them away from the fire or
potential sources of electrical sparks, and use them in a drafty room as possible as you can.
5. Don’t use organic solvent to wipe the non-optical elements, when you need to clean, use the
soft detergent, please.
6. When using, if the microscope is splash by liquid, cut off the power at once, and wipe up the
moisture.
7. Do not disassemble any parts of the microscope. That will affect the function or decline the
performance of the microscope.
8. Place the instrument in a cool, dry position. After using the microscope, remember to cover it
with dust helmet.
9. Operating environment requirements for microscope:
a) Indoor use;
b) Temperature 5 ℃ ~ 30 ℃, humidity 45% ~ 80%;
c) Atmospheric pressure 86kpa ~ 106kpa, altitude below 2000m;
d) Working voltage 100V ~ 240V, frequency 50 / 60Hz.
10. Requirements for microscope storage and transportation environment:
a) Ambient temperature range: - 40 ℃ ~ + 55 ℃;
b) Relative humidity range: 10% ~ 95%.

4
1. Components Name
A12.1095
Objective
Eyepiece
Viewing Head
Condenser
Nosepiec
e
Main Body
Stage

5
2.Structure Overview
A12.1095
1. Shortcut
2. Adjustment hand wheel
3. Frosted filter switch lever
4. Hand wheel speed control button
5. Condenser adjustment knob
6. Switch button for upper and lower light
7. Brightness adjustment knob
8. Condenser
1
2
3
4
5
6
7
8
9
10
11
12
13
9. LCD touch screen
10. Aperture iris diaphragm adjustment ring
11. Electric stage
12. Extended function board fastening screw
13. Extended function board

6
A12.1095
1
2
3
4
5
6
7
8
9
1
10
11
12
13
14
15
1. Light path selector rod
2. Fastening screw for condenser
3. Condenser adjustment knob
4. Adjustment hand wheel
5. Photo button
6. Filed iris diaphragm adjustment ring
7. Shortcut
8. Tension adjustment ring for lamp housing
9. Ground lever
10. Lamp housing socket
11. Lamp room plug
12. Power socket
13. Main switch
14. USB interface
15. Universal wrench

7
3.Adjustment and Operation
A12.1095
3-1 Turn on the power（Fig.1）
First turn the main switch ①on the back of the
microscope body to the "○" (off) state. Insert the
power cord plug ⑤safely into the socket ④of the
adapter to ensure good contact.
Insert the adapter plug ③safely into the power base
②of the microscope to ensure good contact.
Insert the power cord plug ⑥safely into the power
supply socket ⑦to ensure good contact.
After the power is turned on, turn the main switch ①
to the "-" (on) state.
3-2 Adjust the brightness and switch up and
down illumination（Fig.2）
Turning the brightness adjustment knob according
to the direction indicated by an arrow on the
figure 2, the brightness strengthen.On the contrary,
the brightness decreases
As shown in figure 2, when the switch lever is
push-in, the light on the top is opening, the symbol
is ；When the light switch button is
push-out, the light on the bottom is opening, the
symbol is .
3-3 Light socket（Fig.3）
As shown in Fig.3, there are red dots in the socket
and plug,. Before the plug is inserted into the socket,
the direction should be adjusted until their red dots
are aligned.
The symbols on the left and right sockets are
corresponding with the switch button of light
source.As shown in the figure, is the socket of
upper light source, which is corresponding with the
state of pushing in the knob of switching light source.
is the socket of lower light source, which is
corresponding with the state of pushing out the knob
of switching light source.
If the upper and lower light source switch button
is inconsistent with the state shown in the diagram.
Check the light source plug and make sure the plug is
plugged into the appropriate socket.
Switch button for up
and down illumination
Fig.3
Fig.1
Red dot
Fig.2
Brightness
adjustment knob
①
③
②
④
⑤
⑥
⑦

8
A12.1095
3-4 Placing the Specimen（Fig.4）
Pull out the movable claw, put the specimen
in the corresponding slot, loosen the movable
claw and clamp the specimen.
★Be careful when changing the objective. When
the objective needs to be replaced after observing
the specimen with a short working distance
objective, the objective may collide with the
specimen
3-5 Adjusting the interpupillary distance
（Fig.5）
The interpupillary distance range: 47mm～
78mm.While looking through the eyepieces,
adjust for binocular vision until the left and right
fields of view coincide completely.
3-6 Three Light Path Switching（Fig.6）
Select the desired light path and brightness
ratio as shown in Fig.6
Symbol
Light path
selector knob
position
Eye:Camera
（%)
Pushed in
100：0
middle
20：80
Pulled out
0：100
Fig.5
Fig.4
Symbol
Push-pull rod
Fig.6

9
A12.1095
3-7 Focusing（Fig.7）
1. When camera is not used
Push the light path selector rod (as shown in
Fig. 7) in completely and observe with binocular
eyes. Use 10 ×In order to prevent the specimen
from colliding with the objective, the electric
stage should be raised first to make the specimen
close to the objective, and then the focusing hand
wheel should be rotated slowly and reversely to
make the specimen descend, and at 10 ×Search
the image in the eyepiece, and then press the
speed control button to switch to the low rate
focusing gear for fine adjustment. At this time, the
lens can be converted to other magnification
objective to achieve focusing without damaging
the specimen.
2. When camera is needed
Push the light path switch selector rod (as shown
in Fig.7) to the end for binocular observation.
After the image is clear, pull it out again for
observation through the camera system.
The hand wheel buttons on both sides work
independently, and different focusing speeds
can be set respectively. The speed gear is
displayed on the touch LCD screen.
3.Focus upper limit
In actual use, if you want to limit and lock the
stage height, that is, the upper limit position of
focusing according to the situation, you can set
the temporary upper limit position on the touch
screen. Please refer to the touch screen manual for
detailed operation instructions.
Speed control button
Fig.7

10
A12.1095
3-8Adjusting the condenser（Fig.8、9）
The condenser and the objective are coaxial. It
has been adjusted before leaving factory, so the
user needn’t to adjust them by self
The highest position of the condenser has been
adjusted before leaving factory, so the user
needn’t to adjust them by self
Turn the condenser focus knob①to move the
condenser up and down. Raise the condenser when
using the high magnification objective, and
descend it when using the low magnification one.
Centering the Condenser：
1. Adjust the condenser focus knob①to the
highest position.
2. Focus the specimen with 10×objective.
3. Rotate field iris diaphragm ring④until the
image of field iris diaphragm can be observed.
4. Adjust the condenser focusing knob①to
focus the image of field iris diaphragm.
5. Rotate the centering screw③by Allen wrench
to center the field iris diaphragm.
6. Gradually open the field iris diaphragm until
the image of the field iris diaphragm inscribed
with the field of viewing. That means the
condenser is centred correctly.
7. Slightly increase the field iris diaphragm in
reality operation to make the image circumscribed
with the field.
AdjustingAperture Iris Diaphragm
The aperture iris diaphragm is designed for the
adjustment of the numerical aperture, not for the
brightness. Generally, setting the aperture iris
diaphragm to 70- 80% of the N.A. of the objective
in use will provide an image with good contrast. If
you want to observe the image of the aperture iris
diaphragm, remove one eyepiece and look
through the tube. You will see a dark circle
encroaching on the bottom of the tube.
Adjusting the Field Iris Diaphragm
When using, turn the ring④to reduce the field iris
diaphragm, look into the field, if the diaphragm
image is faintness, and do the follow steps: first,
turn the condenser focus knob, shift the condenser
holder to the position where the observed image of
the field of view is sharp; then open the field
diaphragm, let the image full of the field of view,
reduce the mixed light, improving the quality of
the image.
①
②
③
Fig.8
④
Fig.9

11
A12.1095
3-9 Using the filter（Fig.10）
In the normal using state , the frosted filter is at
the push-in state.For dark field observation or
phase contrast observation, press the lever to cut
the frosted filter out of the light path and increase
the brightness of view.
3-10 Using the extensional function
board （Fig.11）
As shown in Fig11, the nosepiece reserves a slot
to hold the extensional function board. The
ordinary bright and dark field board has been inset
the slot before the microscope leaving factory. If
using other extensional function board, only need
to loosen the screw ①, pull out the standard bright
and dark field board, then inset the extensional
function board into the slot and tighten the screw
②.
3-11 Computer control signal interface
and photo function key (Fig. 12、13)
Connect the external digital camera
supported by the company to the computer,
and connect the computer control signal
output interface (as shown in FIG. 13) on
the back of the microscope to the computer.
Meanwhile, the computer runs
scopeimage10.0 software. After capturing
the real-time image of the camera, press the
photographing function key in Figure 12,
The picture captured by the current camera
will be recorded immediately (see the
software operation manual for the operation
application on the computer).
①
②
Fig.11
Photo button
USB
interface
Fig.13
Frosted filter switch lever
Fig.10
Fig.12

12
A12.1095
3-12 Additional function keys (Fig.14)
As shown in Figure 14, there are two function
keys on both sides of the microscope. In the right
function key area, the upper function key is to
switch the converter clockwise and the lower
function key is to switch the converter
anticlockwise; In the left function key area, the
upper function key is the relative coordinate zero
point, and the lower function key is the temporary
upper limit.
⚫This series of electric microscopes has a set
of supporting software. For the specific
operation of the software, please refer to the
software manual
⚫The function keys can be used in
combination with the touch screen. Please
refer to the operation manual of the touch
screen for specific operation
3-13 Touch screen（Fig.15）
As shown in Fig.15, there is a touch screen in
front of the microscope, which can be used for
focusing, adjusting light intensity, setting limit
and converting objective lens. See the operation
manual of the touch screen for the specific
operation method.
Fig.14
Up function key
Down function key
Right function
key area
Left function
key area
Touch screen
Display interface

13
4．Installation
A12.1095
4-1 Installing Diagram
The following figure shows the installation sequence of the components. The number in the
figure shows the assembly steps.
Before installing, be sure every components is clean, do not score any parts or glass
surface.
Keep well with hexagon wrench provided. When replacing the components, you will
need it again.
①
②
⑧
③
⑤
④
⑥
⑨
⑦
Fig.15

14
⚫The light box shown in the display interface No. 9 is directly installed on the rack, so the
customer does not need to install it by
himself.
4-2Installation Steps
A12.1095
Fig.2
Ⅰ
4-2-1 Installing the stage holder（Fig.1、2）
Turn the hand wheel so that the dovetail of the
frame rises until the limit screw exposure end face
as shown in the figure, then install the stage
holder according to the path①in Fig.1. Align the
dovetail groove of the stage holder with the
dovetail bulge of the frame and slide down
naturally until lower limit screw is limited. Then
the screw I in Fig.2 is screwed inward with a
wrench.
4-2-2 Installing the mechanical stage
（Fig.3、4）
The electric stage is installed according to the
path of ②in Fig. 3. First, align the center of the
platform hole with the center of the bracket circle,
and then cover the stage down to the positioning
ring of the bracket.
After the stage is placed stably, screw the screw at
II in Fig. 4 inward with the universal wrench of
microscope belt, so that the platform can not
rotate and shake.
Fig.3
9
②
Fig.1
①

15
A12.1095
4-2-3 Installing the nosepiece（Fig.5）
Mount the nosepiece according to the assembly
path③in Fig.5 into the dovetail of the microscope
and push it to the bottom, then tighten the screw of
Ⅲwith universal wrench to fasten.
4-2-4 Installing the viewing head and
photo attachment/ C mount 0.5×（Fig.6）
1．Installing the trinocular viewing head
Mount the trinocular viewing head according
to the assembly path⑤in Fig.6 into the
round dovetail of the microscope and tighten
the screw IV to fix the viewing head;
2. Installing the photo attachment or
C mount 0.5×
Mount the photo attachment or 0.5×C mount
according to the assembly path⑥into the
connecting seat of the trinocular viewing head and
tighten the screw V;
During the installation of the viewing head,
one hand should always hold the viewing head
to prevent it from falling or being broken
When the trinocular viewing head is not in
use and the photo attachment is not installed,
please cover the interface of the trinocular
viewing head and eyepiece with the
corresponding dust cover to prevent dust.
4-2-5 Installing the eyepiece（Fig.7）
Insert the eyepieces into the eyepiece tube until
they are against each other according to the path
⑦shown in Fig.7.
Fig.5
Ⅲ
③
Ⅴ
C mount 0.5×
Photo attachment
Fig.6
⑤
⑥
Ⅳ
Fig.4
Ⅱ

16
A12.1095
4-2-6 Installing the Objective（Fig.8）
1. Adjust the focusing hand wheel until the
electric stage bracket drops to its lowest limit.
2. Touch the objective key on the LCD screen
from left to right, and screw on the corresponding
objective in the hole position of the corresponding
objective according to the objective magnification
displayed on the key (as shown in Figure 8).
The magnification of the objective key on
the touch screen can be set by yourself.
Please refer to the touch screen instruction
manual for specific operation.
Clean the objectives regularly, for lens is
susceptible to dust.
When operating, use 10×magnification
objective to search and focus specimen
firstly, then replace with higher
magnification objective if necessary.
When replacing the objective, turn the
nosepiece until you hear “clicked”, which
means the objective is in the required
position—center of the light path.
4-2-7 Installing the condenser（Fig.9）
Lower the small bracket to the lowest position,
then insert the condenser into the small bracket
according to the path shown in Figure 9, and push
it to the inmost position. Then screw the screw at
the number VI to support the condenser.
Fig.8
Fig.9
⑧
Ⅵ
Fig.7
⑦

17
A12.1095
4-2-8 Replacing the camera interface
（Fig.10、11）
As shown in the Fig.10, spin out the Ⅶbolt a
distance in the direction shown by the arrow and
don’t tight the photography interface, then spin
out it.
As shown in the Fig.11, screw the camera
interface into the ternary camera barrel to an
appropriate position. Then screw in the number
XI bolt to fasten the camera interface.
In order to ensure the appropriate position
of camera interface, the specimen can be
viewed with an eyepiece and adjusted to a clear
position. Then, the camera is installed to
observe the image, meanwhile, adjust the
position of camera interface accordingly,
tighten the screw Ⅶwhen the clearest image
can be get.
Fig.10
⑩
Fig.11
Ⅶ

18
5．Technical specifications
A12.1095
1. Main Technical Specification
Optical system
NIS60 Infinite optical system
A12.1095
Viewing head
SeidentopfTrinocular Head，Inclined at 30°，Interpupillary
47-78mm
●
Eyepiece
Extra Wide Field Eyepiece EW10X/25
●
Nosepiece
Electric Sextuple Nosepiece
●
Objective
Infinite Plan Objective:4×，10×，20×，40×，100×
●
Focusing
Electric focusing mechanism，fine division 200 nm
●
Stage
Electric stage 270×210mm，Moving range 125×75mm
●
Illumination
3W LED lamp
●
Condenser
Abbe condenser NA0.9/0.1
●
Software
Image software Scopeimage10
●
Photo attachment
○
Video attachment
C Mount 1×
○
C Mount 0.5×
○
Polarization set
○
Turret phase
contrast kit
○
Dark field
attachment
○
Fluorescent
attachment
○
NOTE: ●standard outfit;〇optional
2. Objective Parameter
3. Electrical parameter
1) Input voltage:VAC100-240V 50/60 Hz
2) Lighting:3W S-LED
Magnification
Numerical
Aperture(N.A）
Focal Length
（mm）
Cover glass
thickness
Conjugate
distance（mm）
4X
0.10
30
-
∞
10X
0.25
10.2
-
∞
20X
0.40
6.4
0.17
∞
40X
0.65
0.7
0.17
∞
100X
1.25oil
0.2
0.17
∞
图27

19
6．Troubleshooting
A12.1095
6.1 Optical Part
TROUBLE
CAUSE
SOLUTION
The edge of the field
of view is dark or
the brightness is not
uniform
The nosepiece is not in the located
position( The objective is not in the
center of the light path )
Adjust it into the located
position( turning the objective to
let it in the center of the light
path correctly)
The condenser is not centered
Adjust it to center
A lens (the objective, condenser,
eyepiece or collector) is dirty.
Clean it thoroughly
Find dust and stain
in the field of view
A lens (the objective, condenser,
eyepiece or collector) is dirty.
Clean it thoroughly
There are stains on the slide
Clean it thoroughly
The position of condenser is too low
Adjust the height of the
condenser
Bad image quality
(low-resolution, bad
Contrast)
No cover glass on the specimen
Add cover glass
The cover glass is too thick or too
thin
Use standard cover glass with
thickness of 0.17mm
The specimen is on the reverse side
Turn it over
Dry objective has oil on it.
(especially for 40X objectives)
Wipe the oil
A lens (the objective, condenser,
eyepiece or collector) is dirty.
Clean it
Immersion oil is not used with the
100x objective
Use specified oil
Air bubbles existed in the
immersion oil
Eliminate the bubble
Use the unspecified oil
Use the specified oil
The opening of Aperture diaphragm
is too large
turn it down to the proper size
There are stains on the incidence
lens of the binocular head
Clean the lens
The opening of Aperture diaphragm
is too small
Opening it to the proper size
The position of condenser is too low
Adjust its position
One side of the
image darkens
The condenser is not in the center of
the field or the condenser incline
Reset the condenser and adjust
the Central bolt of the condenser
carefully
The nosepiece is not in the located
position
Rotate the nosepiece to the
required position
The specimen is in the floating state
Fix it firmly
The image move
when focus it
The specimen is floating on the
stage
Fix it firmly
The nosepiece is not in the located
position
Rotate the nosepiece to the
required position