Owlstone Medical ultraFAIMS-T1 User manual

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Owlstone ultraFAIMS-T1 Hardware Installation User Manual

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Contents
Copyright...........................................................................................................................................................3
Disclaimer..........................................................................................................................................................3
Notice of Proper Use of Owlstone Medical Ltd Instruments........................................................................3
Safety notice .....................................................................................................................................................3
Contacting Owlstone Medical Ltd...................................................................................................................4
System Description..........................................................................................................................................5
SYSTEM FAMILIARISATION............................................................................................................................5
SAFETY INFORMATION...................................................................................................................................8
INSTALLING THE T1 SYSTEM.........................................................................................................................9
GETTING STARTED USING ULTRAFAIMS...................................................................................................17
REMOVING THE ULTRAFAIMS UNIT............................................................................................................17
CHANGING A CHIP SEALER ASSEMBLY....................................................................................................18
TROUBLESHOOTING.....................................................................................................................................22
Status LED Indications ..................................................................................................................................22
Technical Support ..........................................................................................................................................22
APPENDIX –OPERATING CONDITIONS......................................................................................................22

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Copyright
©2018 Owlstone Medical Ltd. All rights reserved. Owlstone Medical Ltd provides this user manual to
its customers to use for controlling an ultraFAIMS-T1 system. This manual is copyright protected and
no part of this publication may be reproduced, transmitted, transcribed, stored in a retrieval system
or translated into any language or computer language, in any form or by any means, without the
prior written permission of Owlstone Medical Ltd
Disclaimer
Owlstone Medical Ltd makes no representations or warranties, either expressed or implied, with
respect to the contents hereof and specifically disclaims any warranties, merchantability or fitness for
any particular purpose. Furthermore, Owlstone Medical Ltd reserves the right to revise this
publication and to make changes from time to time in the contents hereof without obligation of
Owlstone Medical Ltd to notify any person of such revision or changes.
Notice of Proper Use of Owlstone Medical Ltd Instruments
The supplied system is in compliance with international regulations. If this system is used in a
manner not specified by Owlstone Medical Ltd, the protection provided by the system could be
impaired
Safety notice
Always observe the following safety precautions
Use only the mains adaptor and leads supplied.
This equipment is for use in moderate climates only (see Appendix). Do not use the equipment in
damp or wet conditions.
Avoid excessive heat, humidity, dust & vibration.
Do not use where the equipment may be subjected to dripping or splashing liquids.
Do not block the cooling duct whilst system is in operation.
Ensure the system is properly installed on the mass spectrometer according to the
enclosed instructions before attempting to power on. Never operate the system unless
properly installed on the mass spectrometer
Do not operate the ultraFAIMS system without an ultraFAIMS chip or test load attached.
Caution: risk of electric shock. Remove power from system before disconnecting from the
mass spectrometer. Do not remove covers.
The ultraFAIMS control unit is rated 24VDC, 60W max. It is fitted with a fuse of type
F3.15AH 250VAC fast-acting. Replace only with a fuse of the same type and rating.
PLEASE READ THIS DOCUMENT BEFORE SETTING UP AND POWERING THE
SYSTEM

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Contacting Owlstone Medical Ltd
Visit the OWLSTONE support website (http://support.owlstonenanotech.com) for up to date contact details
and service support.
UK Office:
183 Cambridge Science Park,
Milton Road,
Cambridge
CB4 0GJ
Tel: [phone]
US Office:
Owlstone Inc.
Suite#202
19 Ludlow Road
Westport, CT 06880
USA
Tel: [phone]

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System Description
The Owlstone ultraFAIMS products are field-asymmetric waveform ion mobility (FAIMS) pre-separators for
use on Mass Spectrometers. They are used to separate or filter ions before they enter the mass
spectrometer. This may provide a reduction in chemical noise or allow observation of ions that would
otherwise be difficult to resolve in the mass spectrum.
The ultraFAIMS-T1 system the version intended for use on Thermo Exactive or Orbitrap Mass
Spectrometers. These notes are provided to assist with understanding how to set up and use the system
safely.
See the Owlstone ultraFAIMS user interface software manual (OW-003401-TM) for further information on
FAIMS separation.
System Familiarisation
The system consists of the following items:
1. UltraFAIMS Control Unit (01-0480)
The control unit generates the FAIMS waveforms needed to
drive the FAIMS chip.
It is controlled by the PC software supplied.
2. UltraFAIMS Chip Sealer Assembly
(or Module)
The chip sealer assembly (shown here inside the packaging) is
the core of the ultraFAIMS system –this is the FAIMS device
that produces the separation of ions as they pass through the
fields applied across the channel.

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3. Modified Back Cone (01-0532)
The modified back cone provides the interface between the
FAIMS chip sealer assembly and the MS inlet and routes the
FAIMS waveforms to the chip.
The chip cone (cap) –below - holds the chip sealer assembly in
place on the back cone interface.
4. Chip Cone (Cap) (01-0401)
The chip cone (cap) holds the chip sealer assembly in place on
the back cone interface.
A chip sealer assembly is shown fitted in the center.
5. Adaptor Plate (01-0400)
The adaptor plate (ring) is the interface between the mass
spectrometer, the FAIMS module and the ionisation source.
6. Connection Extender (01-0355)
The connection extender is the interface between the mass
spectrometer, and the ionisation source extending the gas and
electrical connections to create the extra space needed for the
FAIMS system to be fitted.

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7. Waveform (RF) feeder (01-0399)
The RF waveform feeder connects the chip to the control unit so
that the FAIMS waveforms can be applied. (Interlock sensor
assembly is attached).
8. Support extender pin (01-0479)
The support extender pin fits over one of the ionisation source
support pins to allow the source to be supported when the
adaptor plate is in place.
9. Power supply (01-0320)
The power supply consists of a 24V power supply “brick” with a
custom connector.
10. External trigger cable (13-0165)
Information on how to use the external trigger cable is available
on the Owlstone support website.

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11. THE PTFE Gasket (02-1490)
The PTFE Gasket is used to provide a gas seal for the sweep
gas, but may not be required.
12. USB cable (50-0974)
A standard USB A-to-B cable is used to connect the control unit
to a PC.
Safety Information
Although FAIMS voltages can be hazardous, the ultraFAIMS-T1 system has protection in place to prevent
users being exposed to any hazardous voltages during normal operation. Users should take note of the
following:
When a FAIMS sweep is underway, the RF voltages applied to the chip via the RF waveform feeder
are considered hazardous live.
An interlock on the RF waveform feeder ensures that these voltages are disabled when the RF
waveform feeder is not connected to the adaptor ring. Do not override this interlock. This would
result in hazardous voltages being accessible on the pins of the DF feeder.
The ultraFAIMS system is not designed to operate without a chip sealer assembly installed.
Do not try to run the system without
othe chip sealer assembly (chip module) present in the modified back cone interface
othe chip cone (cap) in place on the modified cone or
othe cone interface mounted on the mass spectrometer.
as this could potentially expose the user to hazardous voltages or cause damage to the ultraFAIMS
system.
Before installing the adaptor plate check that the high voltage interlock plunger moves freely on the
mass spec (see below)
Push plunger fully in
- check it springs
back when released

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Installing the T1 System
1. Remove any ionisation
sources from the mass
spectrometer and
remove the ion sweep
cone from the MS inlet.
Check the MS user
manual to discover how
to do this.
2. As supplied, the chip
cone (cap) is screwed
onto the T1 back cone.
Unscrew this.
Do NOT fit the PTFE
gasket at first as it may
not be needed. The
image shows how it
should be fitted IF
REQUIRED
Do NOT tighten the
screws holding the
internal insert in place –
this must be slightly
loose when fitted to the
mass spectrometer).
Leave both
screws loose
by 2-3 turns
PTFE Gasket

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3. Fit theT1 back cone on
the MS inlet as shown.
The sweep gas intake
pipe fits into the sweep
gas port above the spray
cone
4. Tighten the 3 grub
screws on the T1 back
cone to secure it in place
using the supplied
1.5mm hex key.
To ensure a good seal
around the rear face of
the T1 back cone, push
the cone towards the MS
inlet while tightening the
grub screws.
Tighten (finger tight)
all 3 grub screws and
work around the T1
back cone to retighten
and check them.
5. Choose the chip type to
be used (NC or ND) and
insert the chip sealer
assembly into the chip
cone (cap), aligning the
notch feature as shown
Check the chip for any
visible particles on the
(gold coloured) chip –
remove if possible.
Sweep gas port
Sweep gas pipe
T1 Back Cone
Align location
features

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6. Fit the chip cone,
aligning the location
features on the chip
cone with those on the
T1 back cone
.
7. Push the chip cone
innerinto position over
the o-ring.
It should stay in position
on the T1 back cone.
8. Screw in the chip cone
(cap) outer part and
tighten.
Tightening this
sufficiently is essential
as this pushes the small
o-ring inside the T1
back cone onto the
capillary on the mass
spectrometer to form a
seal.
Locating pin for
cap
Alignment feature
for locating cap
onto cone

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9. Fit the adaptor plate
(ring) onto the inlet –this
connects in the same
way as an ionisation
source, with the
connector block
connecting to the
sockets above the inlet
and the mounting rods
fitted to the side catches.
10. Rotate the rods to hold
the adaptor plate (ring)
in place.
The rods can be stiff to
turn and the left hand
one may require some
force to rotate. Adjusting
the latch blocks forward
and using metal shims
behind them may help.
Ensure the large o-ring
is in place in the groove
on the outer face.
11. Fit the connection
extender above the
adaptor plate (ring)
o-ring

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12. Fit the support
extender pin over the
bottom left source
support pin on the mass
spectrometer.
13. If not already done, fit
the waveform (RF)
feeder to the control
module using the M3x12
screws supplied.
Note the orientation of
this part - with the slope
of the metal to the
outside of the box to get
the correct alignment of
the pins on the bottom of
the waveform (RF)
feeder
14. Use the supplied 2.5mm
hex key to tighten all 4
screws.

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15. Check that all three of
the replaceable pogo
pins are fitted in the
feeder.
16. Insert the connector of
the waveform feeder into
the socket on the right
side of the adaptor
plate (ring)
17. Adjust the extendable
feet on the bottom of the
control module so that it
sits on the bench.
If extra height is needed,
a stand can be provided.
Pogo pin

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18. Use the screws supplied
to secure the waveform
(DF) feeder connector
onto the adaptor plate
(ring).
Ensure there is no
visible gap (> 0.2mm)
between the waveform
(DF) feeder and the
adaptor plate (ring)
19. The ionisation source
can now be mounted
onto the adaptor plate
(ring), in the same way
it is normally attached to
the mass spectrometer
The latch blocks are set
to be 1.7mm from the
face, but may need
adjusting if the ionisation
source doesn’t fit.
A Vernier calliper could
be used to check that it
they are parallel to the
front face.
Insert M3x10 screw
Latch block
Insert M3x35 screw

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20. Connect the USB cable
from the PC to the
control module.
21. Connect the external
power supply to the
control module and plug
in the power supply to
the mains supply. Check
the orientation of this
plug as it can be forced
on the wrong way
around.
22. Connect the interlock
cable attached to the
waveform (DF) feeder to
the Interlock socket on
the control module.
23. If using the external
trigger cable, connect
this to the socket
labelled MS Interface.
24. Install the PC interface
software
See
OW-003401-TM
Owlstone UltraFAIMS
control software
manual
for details of how to do
this.
This software is
available by request
from the Owlstone
support website.

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Getting Started using UltraFAIMS
For general information on the use of UltraFAIMS please see the UltraFAIMS Training Material on the
support website
https://support.owlstonenanotech.com/hc/en-us/sections/202712206
Removing the UltraFAIMS unit
To remove the UltraFAIMS unit from the mass spectrometer, please follow the above steps in reverse.
In particular, note that the adaptor plate (ring) and T1 back cone cannot be removed until the waveform (RF)
feeder connector has been pulled out of the adaptor plate (ring).
Fuse
Power connector
Power switch
External
trigger/comms
connector
Interlock
connector
USB to PC
connector

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Changing a Chip Sealer Assembly
To remove a chip sealer assembly from the system, for cleaning or to replace it with another chip:
1. Unscrew the chip cone (cap) from the T1 back cone
2. The PTFE chip sealer assembly is held in place in the cap by a single catch. To remove the chip
sealer assembly, depress the catch (which can be accessed via the hole in the inner ring of the cap)
using tweezers or other suitable tool, and lever the PTFE mount out. IMPORTANT: do not try to
remove by pulling on the gold connector pins as this may damage the chip.
z
UltraFAIMS chip
sealer assembly
(module)
Depress catch
and lever chip
sealer assembly
upwards

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3. The chip sealer assembly can be cleaned by immersing in suitable solvents and sonicated, or by
heating (up to 150C).
Exposure to vacuum (e.g. in vacuum oven) is not recommended and may distort the chip mount.
Ensure the chip sealer assembly is completely dry before applying FAIMS waveforms to the cleaned
chip.
Further advice on cleaning can be found on the Owlstone support website
https://support.owlstonenanotech.com/hc/en-us/categories/201660986
4. To replace the chip sealer assembly in the cap, align the alignment feature (bump) in the PTFE
mount with the corresponding recess in the inner ring of the cap and push it into place.
Handle chip
sealer assembly
by edges NOT
connector pins
Align location
features

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5. Align the gold connector pins with the holes in the T1 back cone and the alignment feature on the
inner ring of the cap with the locating pin in the T1 back cone.
Locating pin for
cap
Alignment feature
for locating cap
onto cone