Pickering Laboratories PCX 5200 User manual

Operation Manual
PCX 5200
Post-column
Derivatization Instrument
Pickering Laboratories, Inc.
1280 Space Park Way
Mountain View, CA 94043
(800) 54-3330
International: ( 50) 94- 700
Fax: ( 50) 9 8-0749
Website: http://www.pickeringlabs.com
E-mail: [email protected]
Cat. No. 0101-0001
Version , January 2002
© Pickering Laboratories, Inc.
PI
C
KERIN
G
LAB
O
RAT
O RIE
S

PCX 5200 User’s Manual Contents

PCX 5200 User’s Manual Contents
Contents
Please Read ..................................................................................................BYS-1
Symbols.........................................................................................................BYS-1
Chapter 1 • Introduction
Why Post-coIumn Derivatization? ...................................................................... 1-1
How Does It Work?............................................................................................. 1-1
What Are the Requirements? ............................................................................. 1-1
Pickering PCX5200 ............................................................................................ 1-2
Reagent Delivery......................................................................................................................1-2
Column Protection....................................................................................................................1-2
Supplement—The Elements of an HPLC with Post-column System.................. 1-4
Design of an HPLC ..................................................................................................................1-4
A Simpler View of an HPLC .....................................................................................................1-5
Designing a Post-column System ............................................................................................1-5
Chapter 2 • PCX5200 System Components
PCX5200 Post-column Instrument ..................................................................... 2-1
Getting to Know Your PCX5200 ......................................................................... 2-1
Front Panels .............................................................................................................................2-1
Internal .....................................................................................................................................2-3
Rear Panel ...............................................................................................................................2-4
Column Oven ...........................................................................................................................2-6
Reagent Reservoirs .................................................................................................................2-8
External Reagent Pressurization System ................................................................................2-9
Keypad and Liquid Crystal Display User Interface ................................................................2-10
Keypad Examples............................................................................................. 2-13
Loading a Preset ....................................................................................................................2-13
Saving a Preset......................................................................................................................2-15
Flow Diagrams.................................................................................................. 2-18
PRESET Key..........................................................................................................................2-18
PROG Key..............................................................................................................................2-18
Specifications ................................................................................................... 2-20
Wetted Materials, PCX5200...................................................................................................2-20
PCX5200 PEEK .....................................................................................................................2-20
Ratings ...................................................................................................................................2-20
RS232 Serial Port .................................................................................................... ....2-21
Downloading firmware............................................................................................................2-21
Radio frequency .....................................................................................................................2-21

PCX 5200 User’s Manual Contents
Chapter 3 • Installation
Unpacking........................................................................................................... 3-1
Site Requirements .............................................................................................. 3-1
HPLC System Requirements ...................................................................................................3-1
Space Requirements................................................................................................................3-2
Electrical Requirement .............................................................................................................3-2
Inert Gas...................................................................................................................................3-2
Miscellaneous Supplies............................................................................................................3-2
Installation of the PCX5200 ................................................................................ 3-2
Layout of the HPLC and PCX5200 ..........................................................................................3-2
Note About Fittings...................................................................................................................3-3
Inert Gas...................................................................................................................................3-3
Reagent Reservoir Connections ..............................................................................................3-3
Pressure-interlock Connections ...............................................................................................3-4
Injector Connections ................................................................................................................3-6
Detector Connections...............................................................................................................3-6
Guard & Analytical Column Installation....................................................................................3-7
Reagent Pump .........................................................................................................................3-8
Piston-wash System.................................................................................................................3-8
Priming the Reagent Pump......................................................................................................3-9
System Testing & Verification...................................................................................................3-9
Shutdown.......................................................................................................... 3-10
Short-term ..............................................................................................................................3-10
Medium-term .......................................................................................................................... 3-11
Long-term ...............................................................................................................................3-12
Chapter 4 • Routine Maintenance & Troubleshooting
Initial System Testing.......................................................................................... 4-1
Test Chromatogram..................................................................................................................4-1
Paramater Log .........................................................................................................................4-1
Precautions & Problem Prevention..................................................................... 4-2
Mobile Phase ...........................................................................................................................4-2
Back-flow Prevention ...............................................................................................................4-2
Reactor Precautions.................................................................................................................4-2
Electrical Precaution ................................................................................................................4-2
Routine Maintenance.......................................................................................... 4-3
Reagent Pump .........................................................................................................................4-3
Pump Seal Replacement .........................................................................................................4-3
Check-valves............................................................................................................................4-6
Cleaning Check-valves ............................................................................................................4-6
Removing Check-valves ..........................................................................................................4-7
Installing New Check-valves ....................................................................................................4-7
Rebuilding Check-valves .........................................................................................................4-8
Pre-column Filter......................................................................................................................4-9
Reagent Filter.........................................................................................................................4-10
Ambient Reactor (not applicable to single reagent PCX5200) .............................................. 4-11

PCX 5200 User’s Manual Contents
Heated Reactor ...................................................................................................................... 4-11
Fuse .......................................................................................................................................4-12
Troubleshooting Guide ..................................................................................... 4-12
Advice.....................................................................................................................................4-12
When Priming the Reagent Pump is Difficult.........................................................................4-13
Interpretation of Pressures ............................................................................... 4-14
Two-reagent PCX5200...........................................................................................................4-14
One-reagent PCX5200...........................................................................................................4-14
Cleaning the Post-column System ................................................................... 4-15
Fittings....................................................................................................................................4-15
External ..................................................................................................................................4-15
Internal ...................................................................................................................................4-15
Internal Spill ...........................................................................................................................4-15
Appendix A
PCX5200 Parameter Log ...................................................................................A-1
Analytical Conditions ..........................................................................................A-2
Post-column Band-spreading Test......................................................................A-3
Appendix B
Spare Parts.........................................................................................................B-1
References
Limited Warranty
Index

Before You Start
Please Read
BYS-1
• Page 2-8 for the safety requirement of coated-bottles; they must not be substituted!
• Page 3-1 for the HPLC System Requirements, especially for glyphosate or amino
acid analysis! For most ion-exchange applications, the HPLC components must be
compatible with high pH regenerant.
• Pages 2-3 and 3-8 about the piston-wash system of the post-column pump
A note supplies supplementary information which may be helpful or necessary for
better understanding of the material.
The caution calls attention to an operating procedure, practice, or the like, which if not
correctly done or adhered to, could result in loss of information, or damage to, or
destruction of part or all of the equipment. Do not proceed beyond a caution sign until
the indicated conditions are fully understood and met.
Symbols
Please Read

PCX 5200 User’s Manual Before You Start
BYS-2
The following symbols appear on the PCX5200 or its accessories.
This warning sign denotes a hazard. It calls attention to a procedure, practice, or the like,
which if not correctly done or adhered to, could result in injury or loss of life. Do not
proceed beyond a warning sign until the indicated conditions are fully understood and met.
Ce symbole est un signal de danger. Il indique qu’une manipulation, si elle n’est pas
respectée ou effectuée correctement, risque d’entrainer des blessures, voir la mort.
Dieses Warnsymbol kennzeichnet eine Gefahr. Es macht aufmerksam auf einen Vorgang,
eine Handhabung oder ein Vorhaben, die bei unkorrektem Befolgen der Vorschriften zu
einer Verletzung oder einer lebensgefährlichen Situation führen können.
El signo de atención indica un riesgo. Requiere atención sobre un procedimiento, práctica,
o similar, que, si no se ejecuta correctamente o se sigue minuciosamente, prodía producir
heridas o muerte. No continúe a partir de un signo de atención hasta que no se hayan
entendido y alcanzado completamente las condiciones indicadas.
Questo avvettimento informa del pericolo. Molto attenzione riguardante il moto di usare
questa macchina émolto inportante altrimenti risulterádanni e anche morte. Non
continuare di piu affinchéle condizioni e instruzioni sono completamente chiare.
!

PCX 5200 User’s Manual Before You Start
BYS-3
This warning sign denotes a hot surface, a high temperature hazard. It calls attention to a
column heating block hotter than 70°C. For your safety, wear insulating gloves when the
column oven is warm.
Ce symbole indique une surface brûlante. Il signifie que la résistance chauffante de la
colonne a atteint une température supérieure à70°C. Pour votre sécurité, prière de porter
des gants isolants.
Dieses Warnsymbol kennzeichnet eine heiße Oberfläche oder eine Gefahr durch hohe
Temperaturen. Es macht aufmerksam auf den Heizblock des Saulenofens, der heißer als
70°C sein kann. Zu Ihrer Sicherheit sollten Sie isolierende Handschuhe tragen, wenn der
Saulenofen warm ist.
Este signo de atención indica una superficie caliente, un riesgo de alta temperatura. Pide
atención sobre un bloque calefactor de columnas por encima de 70°C. Para su seguridad
use guantes aislantes cuando el horno de columnas estécaliente.
Questo avvettimento informa della temperatura molto alta che possibilmente potrebbe
bruciare. Molto attenzione énecesaria specialmente al blocco caldo della colonna che é
superiore ai 70°C. Per essere protetti énecesario usare guanti insulanti per questa
applicazione.

PCX 5200 User’s Manual Before You Start
BYS-4
Power On
En marche
An
Escendido
Acceso
Power Off
Éteint
Aus
Apagado
Spento
Fuse Specification
Spécification du fusible
Spezifikation der Sicherung
Fusible
Valvole Specificazioni
Protective Ground
Prise de terre
Erdung
Masa de protección
Protezioni a terra

1-1
Chapter 1
Introduction
Post-column derivatization, also known as post-column reaction, renders visible certain
compounds that are normally invisible. This trick is accomplished after the separation
by performing a chemical reaction on the substances that gives them an easily
detectable physical property. Typically you use a reaction that produces a strong color or
makes a fluorescent product. You can increase the sensitivity of detection by several
orders of magnitude in favorable cases. Most reagents are selective for a particular class
of substances, so analytes of that class are more easily seen against a complex
background. So, post-column derivatization is used to increase sensitivity and selectivity
in HPLC analysis.
The post-column reaction system mixes the stream of effluent flowing from the HPLC
column with a stream of reagent solution. The mixture usually flows through a reactor
to allow enough time for the chemical reactions to complete. If the reaction is slow, the
reactor may be heated to speed things up. Some reactions need two or more reagents
added in sequence. Finally, the mixed streams pass into the detector, typically UV/VIS
absorbance or fluorescence. Of course, a practical system requires metering pumps,
pulse damper, thermostats, and safety systems to give reliable results.
• Stability of Reagent. The minimum reagent stability sufficient for routine work is one
day. This means that the yield and signal-to-noise ratio for a given sample must
remain constant for at least 8 hours.
• Speed of Reaction. The analytical separation is complete when the reagent is mixed
with the column effluent. Therefore, in order to minimize band spreading, it is
important to keep the time (therefore volume) small between the mixing tee and the
detector. If the reaction is slow (in excess of one minute), an elevated temperature can
be used to decrease the reaction time.
• Reproducibility. Because the reaction is occurring “on the fly,” as the combined
column and reagent stream flows toward the detector, the reproducibility is linked to
the flowrate precision of the pumps and to the temperature. Accordingly, even an
incomplete reaction will be as repeatable as the retention time for any given species.
The completeness of the reaction, then, is not strictly necessary for reproducibility,
but it is important for maximum sensitivity.
• Minimal Detector Response of Reagents. The color or background fluorescence of the
reagent (or its by-products) represents a continuous noise source. Because the reagent
is present in excess relative to the analyte, the analyte’s signal could be obliterated by
the reagent’s strong background signal. The baseline noise is proportional to the
background signal.
Why Post-coIumn
Derivatization?
How Does
It Work?
What Are the
Requirements?

PCX5200 User’s Manual Chapter 1
1-2
• Solubility. All species must remain in solution, including the combined components of
the eluants and the reagent(s), as well as the newly formed derivative(s). Precipitates
can block capillary tubes, burst reactors, and foul detector flowcells.
• Uniformity of flow. The baseline noise is a function of the flow-noise in the eluant and
reagent pumps. Non-uniform flow causes non-uniform mixing leading to modulation
of the background signal which appears as noise. Refractive index noise can be even
more objectionable than absorbance noise.
The PCX5200 does three main things (Figure 1-1):
1. Thermostats the analytical column
2. Delivers the reagent
3. Heats the reaction.
It also has various features to make the analysis more convenient or reliable, and
features to protect the instrument itself from accidental damage.
The pressurized reservoir serves two purposes:
1. It protects air-sensitive reagents from oxidation.
2. It helps the metering pump fill consistently by preventing cavitation.
The pump is a constant speed, variable-stroke piston pump. The cam cycle is two
seconds, which makes for easy pulse damping.
The reagent pressure gauge does two things:
1. It shows the pump pressure for diagnostic purposes.
2. It absorbs pulses by the spring action of the Bourdon tube. There is a packed-bed
restrictor just downstream to provide a load for the pressure gauge so that it will
effectively absorb pulsations.
Referring to Fig. 1-1, the anti-siphon valve prevents the gas pressure in the reservoir
from forcing liquid through the system with the power off. The post-column pressure
gauge is primarily there to monitor the condition of the reactor. There is a 500 psi relief
valve in case there is a blockage in the reactor or detector. The standard reactor is a
PTFE capillary tube 0.011˝ I.D. wrapped on a heated mandrel. The narrow diameter
reduces band-spreading, and the PTFE is corrosion resistant. There is a 100 psi (5 bar)
back-pressure regulator on the exit line from the detector; it suppresses boiling inside
the hot reactor and prevents bubbles from forming in the detector flowcell.
Usually, the post-column reagent will immediately damage the analytical column. To
prevent this, there is a pressure switch connected between the HPLC pump and the
injector that must sense 500 psi (35 bar) before allowing the reagent pump and reactor
to receive power. If at any time pressure is lost because of fault or programmed
shutdown, the PCX5200 will gracefully shut itself off until a human turns it back on.
Pickering
PCX5200
Reagent
Delivery
Column
Protection

PCX5200 User’s Manual Chapter 1
1-3
Figure 1-1
Pickering post-column systems: one- and two-reagent
The PCX5200 does three
main things:
1. Thermostats the column.
2. Delivers the reagent.
3. Heats the reaction.
Post-column
Pump
Reagent
Pressure
Bypass Valve
Reagent Filter (2µm)
Restrictor
Anti-siphon Valve
(40 psi)
Post-column
Pressure
Pressurized
Reagent
Bottle
LC Eluants
LC Pump
Injector Pre-column
Filter (0.5µm)
Thermostatted
Column and Guard Heated Reactor
Detector
Back-pressure
Regulator
(100 psi)
Waste
500 psi
Pressure Switch
Over-pressure
relief valve
(525 psi)
Waste
2
13
Post-column
Pump
Reagent
Pressure
Bypass Valves
Reagent Filters (2µm)
Restrictors
Anti-siphon Valves
(40 psi)
Post-column
Pressure
Pressurized
Reagent
Bottles
LC Eluants
LC Pump
Injector Pre-column
Filter (0.5µm)
Thermostatted
Column and Guard Heated
Reactor
Detector
Back-pressure
Regulator
(100 psi)
Waste
500 psi
Pressure Switch
Over-pressure
relief valve
(525 psi)
Waste
2
13
Ambient
Reactor

PCX5200 User’s Manual Chapter 1
1-4
This next section is a simplified view of a post-column system and it is intended to help
novice HPLC operators.
In order to understand post-column HPLC, we need to understand the design of an
HPLC. If we connect an HPLC pump directly to a detector (with nothing in between),
the baseline from the detector shows a periodic noise (Figure 1-2); the time period is
equivalent to the pump stroke.
The Elements of
an HPLC with
Post-column
Sytem
Design of an HPLC
Figure 1-2
Now add a commercial “pulse damper.” The baseline is still not smooth; the periodic
noise is still there although less pronounced (Figure 1-3).
Figure 1-3
Eluants
LC Pump
Injector
Detector
Waste
Baseline
Pulse
Damper
Eluants
LC Pump
Injector
Detector
Waste
Baseline

PCX5200 User’s Manual Chapter 1
1-5
What we need is a column. The column does more than separation. It creates a back-
pressure. It is the combination of the “pulse damper” and the column that creates a
smooth baseline. (Figure 1-4)
A Simpler View
of an HPLC
Let us use a river as an example. If it rains; the river swells. If it stops raining; the level
goes down. As the level fluctuates, it is equivalent to a periodic noise. To obtain a
constant flow, we need to add a reservoir (“pulse damper”) and a dam (column). The
flow downstream from the dam is constant (smooth baseline).
What happens if we simply add a post-column pump, a mixing tee, and a reactor? What
happens to the baseline? The periodic noise returns (created by the post-column pump;
Figure 1-5).
Designing a
Post-column
System
Figure 1-4
Figure 1-5
Pulse
Damper
Eluants
LC Pump
Injector Pre-column
Filter Thermostatted
Column & Guard
Detector
Waste
Baseline
1
Pulse
Damper
Baseline
Post-column
Pump
Reagent
Bottle
Eluants
LC Pump
Injector Filter Thermostatted
Column & Guard Heated
Reactor
Detector
Back-pressure
Regulator
Waste

PCX5200 User’s Manual Chapter 1
1-6
However we do not need to invent anything new; we just need a “pulse damper” and a
column (at Pickering, we call it a restrictor; Figure 1- ).
Figure 1-6
A Pickering flow-conditioner includes a pressure gauge and a restrictor. The Bourdon
tube inside the pressure gauge is the “pulse damper” and the restrictor packed with very
inert material is the column. With the flow conditioner in place, the baseline is now
acceptable.
Add a second or third post-column reagent? Just follow the same procedure. Another
post-column pump, pressure gauge, restrictor, mixing tee, and reactor.
We add a post-column pressure gauge (Fig. 1-1) to determine if there is any blockages in
the reactors. Note that the pressure in the post-column gauge is stable because it is
down stream from the analytical column and the restrictors (i.e. standing down stream
from the reservoir and dam in our earlier river example).
Now you are ready for Chapter 2.
Post Column
Pressure
Pulse
Damper
Post-column
Pump
Restrictor
Eluants
LC Pump
Injector Pre-column
Filter Thermostatted
Column & Guard Heated
Reactor
Detector
Back-pressure
Regulator
Waste
Baseline
1
Reagent
Pressure Gauge
Reagent
Bottle

2-1
Chapter 2
PCX5200 System Components
The PCX5200 is available for one-reagent or two-reagent, 120V or 240V operation, and
is shipped completely assembled, calibrated, and tested.
The one-reagent PCX5200 consists of a simplex reagent pump, heated reactor, column
heater, backflow and over-pressure safety devices, filters and flow conditioner, reagent
reservoir, Saran® gas tubing, and other accessories. Note that the one-reagent PCX5200
can be easily upgraded to the two-reagent PCX5200.
The two-reagent PCX5200 consists of a duplex reagent pump, heated and ambient
reactors, column heater, backflow and over-pressure safety devices, filters and flow
conditioners, reagent reservoirs, Saran gas tubing, and other accessories.
If this system was purchased to analyze for glufosinate, amino acids, carbamates, or
glyphosate, you should have an appropriate column kit and chemistry manual for your
application.
Terminologies—There are three removable front panels (Figure 2-1). From left to right,
they are referred to as:
1. Post-column panel
2. Reagent 1 flow-conditioner panel
3. Reagent 2 flow-conditioner panel (if applicable)
PCX5200
Post-column
Instrument
Getting to
Know Your
PCX5200
Front Panels • On the post-column panel, the post-column pressure gauge measures the liquid
pressure at the first mixing tee. This is effectively the pressure on the heated reactor,
ambient reactor, and dectector. This gauge indicates pressure when liquid is flowing
through the system.
• The liquid connections to the pressure sensor are labelled “From LC Pump” and “To
Injector.” The pressure sensor is part of the safety interlock system. The sensor
requires 500 psi (35 bar) before the module can be enabled.
• The liquid connection “From Injector” is also the pre-column filter. The filter element
is a 0.5 µm frit (Cat. No. 3102-9042).
• Note: Only PEEK ferrules should be used to connect the tubing from the injector. This
is because the filter is made if a soft PEEK material and a stainless steel ferrule will
damage it.
• “To Detector” bulkhead fitting should be connected to the detector with 0.010 inch
(0.25 mm) ID tubing.
• “Over Pressure Relief” is a safety relief valve that opens in case the post-column
pressure reaches >500 psi (35 bar). This protects the soft fluorocarbon tubing of the
reactors from rupture in the event of a blockage in the post-column system or other
fault. Run a piece of tubing from this fitting to a clean dry beaker. Any evidence of
liquid in this tubing indicates a fault condition.

PCX5200 User’s Manual Chapter 2
2-2
Figure 2-1. Front view
of the two-reagent
PCX5200 system
2000
1000
500 2500
3000
1500
600
400
200 800
1000
500 2000
1000
500 2500
3000
1500
PCX 5200
REAGENT 1
PRESSTATUS
COLUMN
TEMPPUMP
ENABLE
POWER
REACTOR
TEMP
PROG
REAGENT 2
PRE
SET
PICKERING
LABORATORIES
Model PCX 5200
Post-Column Deriv tizer
TO
INJECTOR
FROM
PUMP
FROM
INJECTOR
TO
DETECTOR
OVER
PRESSURE
RELIEF
POST-COLUMN
PRESSURE REAGENT 1
PRESSURE
BYPASS 1
REAGENT 2
PRESSURE
BYPASS 2
600
400
200 800
1000
500
PCX 5200
REAGENT 1
PRESSTATUS
COLUMN
TEMPPUMP
ENABLE
POWER
REACTOR
TEMP
PROG
REAGENT 2
PRE
SET
PICKERING
LABORATORIES
Model PCX 5200
Post-Column Derivatizer
TO
INJECTOR
FROM
PUMP
FROM
INJECTOR
TO
DETECTOR
OVER
PRESSURE
RELIEF
POST-COLUMN
PRESSURE
Figure 2-2
Exposed view of
the two-reagent
PCX5200 system and
post-column pumps

PCX5200 User’s Manual Chapter 2
2-3
• On the Reagent 1 flow-conditioner panel, the Reagent 1 pressure gauge measures the
pressure of the reagent at the outlet of the reagent pump. This gauge is an integral
part of the pulse-dampening system. When the reagent pump is on, the needle swings
over a range of about 800 psi in time with the pump piston.
• The “Bypass” 1 valve is used to purge and prime the first reagent pump. Attach the
20 mL syringe to the Luer fitting in the center of the knob. Open the valve by turning
it counterclockwise about one turn. Apply suction with the syringe to draw reagent
through the pump. Use strong suction to remove bubbles from the Reagent 1 pressure
gauge, from the reagent pump, or from the reagent supply line. Close the valve by
turning it clockwise; only gentle pressure is needed to close the valve. Keep the Luer
fitting clean by rinsing it with water after use.
• If applicable, the Reagent 2 pressure gauge operates the same as the Reagent 1
pressure gauge.
• The “Bypass 2” valve is used to purge and prime the second reagent pump, and it
operates the same as the Bypass 1 valve.
• The three removable front panels gives access to the liquid ends of the pump, reagent
filters, restrictors, the thermostatted reactor, electrical connections for the reagent
pump, and the flow adjustment knobs of the post-column pump. The two reagent
panels can be removed by loosening the captive thumb-screws. The Post-column panel
(left) can be removed by loosening the two captive thumb-screws.
• The reagent pump with a piston-wash system is behind the two reagent flow-
conditioner panels. In normal operation, the pump requires no adjustment. It is
calibrated at the factory to 0.30 mL/min for both channels. The micrometer knobs on
back of the pump adjust the flow rates. One full turn of the knob changes the flow
rate by about 0.1 mL/min.
Important! Make sure the piston-wash system is flushed with 80/20 water/methanol
before turning on the post-column pump.
Figure 2-3.
Post-column pump &
piston-wash system
Internal

PCX5200 User’s Manual Chapter 2
2-4
• The reagent filter is located just downstream of the prime/purge valve in the reagent
panel. The filters and elements are similar in appearance to the pre-column filter, but
they are different. The reagent filter element is a 2 µm frit (Cat. No. 3102-9040).
2000
1000
500 2500
3000
1500
• The power connector is a standard IEC 320 type connector. Use the appropriate
power cord for your local wall outlet and electrical code. The 120V version comes with
a standard North American cord set. The 240V version comes with a cord set used in
much of continental Europe (France, Germany, Benelux, etc.), or your local reseller
may have provided the correct local cord set. If your local power outlets are different,
you will need to obtain the appropriate grounded cord set.
• The main power switch is located in the power connector assembly.
• The fuse holder is located in the power connector assembly. To change the fuse, first
remove the power cord from the connector. Carefully pry out the fuse clip with a small
screwdriver. Replace with the specified-type fuse.
For 120 V systems, use a fast-acting 3 A, 250 V, 5 x 20 mm fuse, type GMA3 (Cat. No.
3543-0045).
For 240 V systems, use two fast-acting 1. A, 250 V, 5 x 20 mm fuses, meeting IEC127
specifications (Cat. No. 3543-0044).
• The serial computer interface is the DB9F connector. See the section later in this
chapter about the “RS232 serial port.”
Figure 2-4.
Reagent filter &
flow-conditioner
Rear Panel

PCX5200 User’s Manual Chapter 2
2-5
Warning. Ensure that the power cord is disconnected before replacing a fuse. Use only
the specified-type fuse.
Attention. Assurez vous que le cable secteur n’est pas connecté avant de changer un
fusible.
Warnung. Sicherungen dürfen nur bei nicht angeschlossenem Netzkabel ersetzt oder
gewechselt werden.
Cuidado. Asegúrese que el cable de red está desconectado antes de instalar o cambiar un
fusible.
Attenzione. Assicuratevi che il cavo di alimentazione sia scollegato prima di installare o
sostituire un fusible.
Waarschuwing. Zorg dat de voedingskabel losgekoppeld is, voordat een zekering wordt
geplaatst of vervangen.
Avvertimento. Fare atenzione che la corda del voltaggio sia staccata prima di cambiare
valvole. Usa solo valvole di capacitá precisata dalla fattoria.
!
I
O
Figure 2-5. One fuse for 120V systems; two fuses for 240V systems
Fuses