cytiva Kvick Lab Cassette Holder User manual

Learning to Use the
Kvick Lab Cassette
Holder
User Manual
cytiva.com

Table of Contents
1Important user information ................................................................................................................ 4
2 About this user guide ........................................................................................................................... 5
2.1 Audience .................................................................................................................................................................................................. 6
2.2 Where to find more information ..................................................................................................................................................... 7
2.3 Safety ........................................................................................................................................................................................................ 8
2.4 Warnings, cautions, and tips ............................................................................................................................................................ 9
3 Kvick Lab Cassette Holder .................................................................................................................. 12
3.1 Unpacking and lifting the cassette holder .................................................................................................................................. 13
3.2 Package contents ................................................................................................................................................................................ 14
3.3 Learning about the holder ................................................................................................................................................................. 15
3.4 Features ................................................................................................................................................................................................... 16
3.5 Learning about cassettes .................................................................................................................................................................. 17
4 Connecting the Holder to Your System ............................................................................................. 18
4.1 Where to install the holder ............................................................................................................................................................... 19
4.2 How to install the holder .................................................................................................................................................................... 20
5 Installing Cassettes in the Holder ...................................................................................................... 21
5.1 Introduction ............................................................................................................................................................................................ 22
5.2 Determining how many cassettes you can install ................................................................................................................... 23
5.3 Installing the cassettes ...................................................................................................................................................................... 24
6 Preparing a Cassette for Use .............................................................................................................. 26
6.1 Introduction ............................................................................................................................................................................................ 27
6.2 Stage 1 – Rinse storage solution from the cassette ............................................................................................................... 28
6.3 Stage 2 – Measuring water flux ....................................................................................................................................................... 30
6.4 Stage 3 – Test the integrity of the cassette ................................................................................................................................ 34
6.5 Stage 4 – Sanitizing the Kvick Lab cassette ............................................................................................................................... 37
6.6 Stage 5 – Conditioning the system with buffer ......................................................................................................................... 38
7 Using the Holder .................................................................................................................................. 40
7.1 Introduction ............................................................................................................................................................................................ 41
7.2 Stage 1 – Starting the cross-flow process .................................................................................................................................. 43
7.3 Stage 2 – Operating the system in a steady state ................................................................................................................... 44
7.4 Stage 3 – Shutting the system down and recovering product ............................................................................................ 45
8 Cleaning and Storing ........................................................................................................................... 47
8.1 Introduction ............................................................................................................................................................................................ 48
8.2 Stage 1 – Cleaning a cassette after use ....................................................................................................................................... 49
8.3 Stage 2 – Measuring water flux ....................................................................................................................................................... 50
8.4 Stage 3 – Storing the cassette and holder .................................................................................................................................. 51
9 Troubleshooting .................................................................................................................................. 52
9.1 Troubleshooting guide ....................................................................................................................................................................... 53
10 Maintenance ......................................................................................................................................... 55
10.1 Inspection ................................................................................................................................................................................................ 56
10.2 Maintenance .......................................................................................................................................................................................... 57
Table of Contents
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10.3 Storing the holder ................................................................................................................................................................................ 58
11 Specifications ...................................................................................................................................... 59
11.1 Size and dimensions ............................................................................................................................................................................ 60
11.2 Materials of construction .................................................................................................................................................................. 61
11.3 Operating parameters ....................................................................................................................................................................... 62
12 Appendix ............................................................................................................................................... 63
12.1 Chemical compatibility ...................................................................................................................................................................... 64
12.2 Cassette sanitizing Agents ............................................................................................................................................................... 65
12.3 Parts and accessories ......................................................................................................................................................................... 66
Index ............................................................................................................................................................ 67
Table of Contents
Learning to Use the Kvick Lab Cassette Holder User Manual 18117269 AI 3

1 Important user information
All users must read this entire manual to fully understand the safe use of Kvick Lab™ Cassette Holder.
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to
proceed until all stated conditions are met and clearly understood.
Note: A note is used to indicate information that is important for trouble-free and optimal use of
the product.
Recycling
Waste electrical and electronic equipment must not be disposed of as unsorted
municipal waste and must be collected separately. Please contact an authorized
representative of the manufacturer for information concerning the decommissioning
of the equipment.
1 Important user information
4 Learning to Use the Kvick Lab Cassette Holder User Manual 18117269 AI

2 About this user guide
This user guide describes how to use the Cytiva Kvick Lab cassette holder. Topics include how to
integrate and connect the holder to your cross-flow system, how to install cassettes, and how to use
and clean the holder and cassettes.
In this chapter
Section See page
2.1 Audience 6
2.2 Where to find more information 7
2.3 Safety 8
2.4 Warnings, cautions, and tips 9
2 About this user guide
Learning to Use the Kvick Lab Cassette Holder User Manual 18117269 AI 5

2.1 Audience
This user guide addresses the needs of scientists, process engineers, and technicians who operate
laboratory and pilot-scale cross-flow systems. The level of information presented in this guide
assumes the user possesses basic laboratory and technical skills, and has the knowledge and
documentation to safely operate any user supplied equipment connected to the Kvick Lab cross-flow
system. If you need assistance with the instructions in this guide, contact Cytiva for more
information.
2 About this user guide
2.1 Audience
6 Learning to Use the Kvick Lab Cassette Holder User Manual 18117269 AI

2.2 Where to find more information
You can consider this user guide as part of a set. When you purchase Kvick Lab cassettes or a Kvick
Lab crossflow system, you receive the other documents in the set (see the list below).
You can download technical documents and learn more about Kvick Lab cassette holders and cross-
flow systems by visiting our website, cytiva.com.
You can also receive customer and technical support by visiting cytiva.com.
Key documents for Kvick Lab products
•Kvick Lab Cross-Flow System: Instructions for Use – Describes how to install, use, and
maintain theKvick Lab cross-flow system, reservoir, feed pump, and accessories. Supplied with
Kvick Lab cross-flow systems.
•Learning to Use the Kvick Lab Cassette Holder — Describes how to use the Kvick Lab cassette
holder. Topics include: installation in a cross-flow system, operation, and cleaning. Supplied with
Kvick Lab cassette holder.
•Cytiva Cassettes: Instructions for Use — Describes basic operational procedures such as
flushing, cleaning, and specifications. Supplied with cassettes.
•MSDSs for glycerin and NaOH — Provides safe handling and disposal information. Available for
printing and downloading from the Cytiva website.
2 About this user guide
2.2 Where to find more information
Learning to Use the Kvick Lab Cassette Holder User Manual 18117269 AI 7

2.3 Safety
Everyone who works with the Kvick Lab cassette holder should read, understand, and follow the
instructions in this user guide and the safety guide entitled: Cross-Flow System Safety: Multilingual
Safety Instructions (provided with your system).
If you do not understand an instruction, you should stop working with the Kvick Lab holder and
contact Cytiva for guidance. You should save the user and safety guides and make them available to
all users of the Kvick Lab holder.
Cytiva designed the Kvick Lab cassette holder for laboratory scale filtration of biological solutions
using membrane cassettes under the conditions stated in this user guide. If you use the holder and
cassettes in a manner not specified by Cytiva, you may impair the protection provided by the holder
and cassettes.
When using any laboratory, pilot-scale, or production cross-flow equipment, the potential exists for
personal injury unless you follow established safety procedures. When using Cytiva products, you
should follow OSHA, federal, state, and local safety regulations for equipment installation and
operation. You should follow your company’s safety regulations. You should follow the specific safety
instructions provided in this user guide and any original equipment manufacturer user guides
provided with your system.
Only qualified personnel who are adequately trained and who understand the operating instructions
should install, operate, maintain, and inspect the Kvick Lab cassette holder.
2 About this user guide
2.3 Safety
8 Learning to Use the Kvick Lab Cassette Holder User Manual 18117269 AI

2.4 Warnings, cautions, and tips
This user guide uses symbols and blocks of text to provide you with safety warnings and other
important information:
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to
proceed until all stated conditions are met and clearly understood.
Tip: A tip contains useful information that can improve or optimize your procedures.
WARNING
If you overpressurize the cassette holder and cross-flow filtration
system with compressed air during integrity testing, system
components can rupture causing bodily harm. To avoid the rupture
of a system component due to over pressurization with
compressed air, use a pressure regulator to limit the pressure of
your compressed air supply to the maximum operating pressure of
your system components. For the Kvick Lab cassette holder and
Kvick Lab system, the maximum operating pressure is 4 barg (60
psig). Wear the appropriate personal protection devices when
testing the system.
If a cross flow system leaks, it can release potentially hazardous
process fluids or cleaning fluids, causing bodily harm. To prevent
leaks and the release of potentially hazardous process or cleaning
fluids, limit feed pressure to 4 barg (60 psig) for Kvick Lab holders
and systems. Periodically check parts for wear and ensure you
assemble the components correctly. To monitor system pressure,
Cytiva recommends that you install pressure gauges on cross-flow
systems as illustrated in this user guide. Isolate and depressurize
your system before disassembling it. Periodically inspect the
system’s gaskets, seals, and connections for wear. Ensure you
assemble the holder correctly following the instructions in this
guide. Wear the appropriate personal protection devices and
clothing when operating and cleaning the system.
2 About this user guide
2.4 Warnings, cautions, and tips
Learning to Use the Kvick Lab Cassette Holder User Manual 18117269 AI 9

WARNING
The cassette and cassette bag contain an aqueous solution
containing 0.1 to 0.2 N NaOH and 20 to 22% glycerin by weight.
When opening the cassette bag, follow the standard safety
procedures for handling aqueous NaOH, including the use of safety
glasses, safety gloves, and protective lab coat.
To avoid unexpected spilling, hold the cassette package upright
over a sink and trim the top of the cassette bag with scissors. Drain
and dispose of the excess storage solution in accordance with
environmental regulations.
To avoid inadvertent contact with the storage solution after the
cassette is removed from the bag, rinse the outside surface of the
cassette with high purity water.
WARNING
Cassette holders are heavy. Dropping a cassette holder on your
feet or hands can cause bodily injury. To prevent injury from
dropping a cassette holder on your feet or hands, ensure you have a
secure, controlled grip and a sufficient number of people — or the
proper equipment — to move the cassette holder. Wear safety
shoes. The weight of cassette holders requires that you support
them well, especially when integrated into a cross-flow system.
Ensure the holder and piping is properly supported and that piping
supports meet local safety codes.
Mishandling potentially hazardous process and cleaning solutions
can cause bodily harm. To safely handle potentially hazardous
process, cleaning, and storage solutions, read the material safety
data sheets for the solutions you use. Follow the material safety
data sheet instructions for safe handling and use the personal
protection equipment required by your company, and local, state,
and federal laws.
CAUTION
If the Kvick Lab system pump runs dry (without any process fluid in
it), you can damage the pump. To prevent damage to the pump,
ensure that fluid is present in the reservoir and feed line whenever
you run the pump.
2 About this user guide
2.4 Warnings, cautions, and tips
10 Learning to Use the Kvick Lab Cassette Holder User Manual 18117269 AI

CAUTION
Using tap water to flush or rinse your cassette can plug the pores of
membrane with the relatively large particulates (rust, dirt, minerals,
bacteria, etc.) found in tap water. To prevent plugging the pores of
the cassette, always use 0.2-μm filtered or water-for-injection (WFI)
when rinsing or flushing the cassette, or when making up cleaning
solutions or adding dilution water to process fluids. In this user
guide, the term clean water means 0.2-μm filtered water or WFI.
2 About this user guide
2.4 Warnings, cautions, and tips
Learning to Use the Kvick Lab Cassette Holder User Manual 18117269 AI 11

3 Kvick Lab Cassette Holder
In this chapter
Section See page
3.1 Unpacking and lifting the cassette holder 13
3.2 Package contents 14
3.3 Learning about the holder 15
3.4 Features 16
3.5 Learning about cassettes 17
3 Kvick Lab Cassette Holder
12 Learning to Use the Kvick Lab Cassette Holder User Manual 18117269 AI

3.1 Unpacking and lifting the cassette holder
Your Kvick Lab cassette holder arrives packed in a cardboard box that includes the torque wrench
and documentation. To unpack the system, place the box on the ground. Open the top of the box and
remove foam insert. Remove the cassette holder, documentation, and torque wrench. When you
transport the cassette holder, hold it firmly with two hands by the stand.
3 Kvick Lab Cassette Holder
3.1 Unpacking and lifting the cassette holder
Learning to Use the Kvick Lab Cassette Holder User Manual 18117269 AI 13

3.2 Package contents
Each shipment of a Kvick Lab cassette holder includes the following components:
•Kvick Lab holder
•Torque wrench, socket, and torque wrench instructions
•User guide
•Certificate of analysis
3 Kvick Lab Cassette Holder
3.2 Package contents
14 Learning to Use the Kvick Lab Cassette Holder User Manual 18117269 AI

3.3 Learning about the holder
The Kvick Lab cassette holder is designed to hold 1 to 5 cassettes. Together, the holder and the
cassettes enable you to separate, concentrate, and diafiltrate biological solutions with precision. For
example, you can complete ultrafiltration at laboratory volumes (starting volumes of less than 1
liter).
3 Kvick Lab Cassette Holder
3.3 Learning about the holder
Learning to Use the Kvick Lab Cassette Holder User Manual 18117269 AI 15

3.4 Features
The Kvick Lab holder simplifies operation with fewer parts and connections than other types of cross-
flow cassette holders. Cytiva cassette holders share the following features: (1) three forward facing
ports for convenience, (2) vertically orientated hardware for best drainage and product recovery, (3)
uses state-of-the-art Cytiva cassettes that include several patented and new-to-market features
that increase safety, facilitate use, and improve process control and product quality, and (4) highly
competitive economics of use.
The main components include a flow distribution manifold, back plate, and tie rods (see the Figure
below). These components hold the cassettes in place and manage the flow of process fluids into and
out of the cassettes. To ensure ease of use, the holder is mounted on a stand and uses industry-
standard sanitary fittings.
The Kvick Lab holder can hold multiple Kvick Lab cassettes providing a maximum membrane area of
0.55 m2 (6.0 ft2). Cytiva manufactures Kvick Lab cassettes with a wide range of molecular weight
cutoffs to meet a variety of applications.
Figure 3.1: Parts of a Kvick Lab cassette holder
3 Kvick Lab Cassette Holder
3.4 Features
16 Learning to Use the Kvick Lab Cassette Holder User Manual 18117269 AI

3.5 Learning about cassettes
The two sizes of Kvick Lab cassettes share design components, including inlet and outlet ports,
labeling, and alignment notches (see the Figure below).
Figure 3.2: The main parts of a Cytiva cassette
3 Kvick Lab Cassette Holder
3.5 Learning about cassettes
Learning to Use the Kvick Lab Cassette Holder User Manual 18117269 AI 17

4 Connecting the Holder to Your System
In this chapter
Section See page
4.1 Where to install the holder 19
4.2 How to install the holder 20
4 Connecting the Holder to Your System
18 Learning to Use the Kvick Lab Cassette Holder User Manual 18117269 AI

4.1 Where to install the holder
The integration of the cassette holder into your cross-flow system depends upon your application
and filtration goals. You can complete many cross-flow objectives using a basic cross-flow
configuration (see the Figure below). To learn about alternate system configurations for special
applications, contact Cytiva.
Retentate
Pump
Valve
Pressure
Feed
gauge Kvick Lab
holder
vessel
Collection
Permeate
Drain
line
Diafiltration Solution
Feed
reservoir
Figure 4.1: Basic cross-flow system configuration
4 Connecting the Holder to Your System
4.1 Where to install the holder
Learning to Use the Kvick Lab Cassette Holder User Manual 18117269 AI 19

4.2 How to install the holder
Gathering materials
Gather the following materials to install your Cytiva cassette holder into your cross-flow system:
•A cross-flow system including a feed reservoir, feed pump, a minimum of two glycerin-filled
pressure gauges, and three flow control valves
•System piping and fittings to connect the feed pump, permeate collection vessel, and feed
reservoir to the holder. The system piping must end in ½-inch sanitary clamps to connect to the
Kvick Lab holder.
•Three, fractional sanitary clamps and ½-inch gaskets
Making the connections
Place the Kvick Lab holder on a sturdy lab bench near your cross-flow system. Stabilize and level the
holder using the adjustable feet. Keep piping runs short to minimize holdup volume. Connect the
holder’s feed, retentate, and permeate ports to your system’s piping using the three gaskets and
sanitary clamps.
4 Connecting the Holder to Your System
4.2 How to install the holder
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