Gilson pipetman User manual

Quick Guide
EN

2QUICK GUIDE |PIPETMAN®
Introduction
PIPETMAN®, recognized as the pipetting standard, is
a fully adjustable, air-displacement pipette that uses
disposable tips. PIPETMAN combines its legendary
accuracy, precision, and durability with easy pipetting
and handling.
PIPETMAN offers a large range of accurate and precise
pipettes with optimized pipetting and purge forces for
hours of pipetting.
Eight single channel models cover a volume range from
0.2 µL to 10 mL.
Eight multichannel models cover a volume range from
1 µL to 300 µL.
Parts Checklist
After unpacking the pipette, verify that the following
items were included and are undamaged:
Single models
●PIPETMAN®
●Quick Guide
●Safety bag
●Certificate of Conformity (including barcode
sticker)
●Dual-position adapter (for P2 and P10 models
only)
Multichannel models
●PIPETMAN®
●Quick Guide
●Safety bag
●Certificate of Conformity (including barcode
sticker)
●Ejector spacers for D10 tips (for Px10 models only)
Good Laboratory Practice (GLP) Compliance
The serial number is engraved on the body of the
pipette. It provides unique identification of your pipette
and the manufacturing date.
Ex:
The barcode on the box
and the Certificate of
Conformity provides
traceability of your pipette.
Figure 1
Serial number and
identification
Year Month Production
number
A A 50001
Serial number

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PIPETMAN® |QUICK GUIDEQUICK GUIDE |PIPETMAN®
Specifications
PIPETMAN® is a high-quality pipette that offers
excellent accuracy and precision. The data provided in
the Maximum Permissible Errors table on page 4 was
obtained using PIPETMAN® DIAMOND Tips.
These values are guaranteed only when genuine
PIPETMAN DIAMOND Tips are used.
With a precise pipetting technique the P2 model
may be used to aspirate volumes as low as 0.1 µL
and the P10 model as low as 0.5 µL.
NOTICE
Description
PIPETMAN®
DIAMOND tip
Tip ejector
Tip holder
Color-coded
push button
Blue ring
Thumbwheel
Tip ejector button
Body or
handle
Connecting nut
Figure 2
PIPETMAN® multichannel and single channel model
components
Clip tip ejector
Cover
Ejector spacer
Ejector lock
Ejector support

4QUICK GUIDE |PIPETMAN®
Maximum Permissible Errors
The data in the following table conforms to the
ISO8655-2 standard.
*CV means Coecient of Variation
**P10 model can be used up to 0.5 L with a precise pipetting technique.
***P5000 and P10mL do not have tip ejectors.
PIPETMAN® SINGLE CHANNEL—VARIABLE VOLUME MODELS
Vol.
(µL)
Maximum Permissible Errors
Gilson ISO 8655
Systematic
Error
(µL)
Random
Error
(µL)
Systematic
Error
(%)
Random
Error
(%CV*)
Systematic
Error
(µL)
Random
Error
(µL)
Systematic
Error
(%)
Random
Error
(%CV*)
P2 (P/N F144054M) WITH D10 AND DL10 TIPS
0.2
0.5
1
2
± 0.024
± 0.025
± 0.025
± 0.030
≤0.012
≤0.012
≤0.012
≤0.014
± 12.0
± 5.0
± 2.5
± 1.5
≤6.0
≤2.4
≤1.3
≤0.7
± 0.08
± 0.08
± 0.08
± 0.08
≤0.04
≤0.04
≤0.04
≤0.04
± 40.0
± 16.0
± 8.0
± 4.0
≤20.0
≤8.0
≤4.0
≤2.0
P10** (P/N F144055M) WITH D10 AND DL10 TIPS
1
5
10
± 0.025
± 0.075
± 0.100
≤0.012
≤0.030
≤0.040
± 2.5
± 1.5
± 1.0
≤1.2
≤0.6
≤0.4
± 0.12
± 0.12
± 0.12
≤0.08
≤0.08
≤0.08
± 12.0
± 2.4
± 1.2
≤8.0
≤1.6
≤0.8
P20 (P/N F144056M) WITH D200 TIPS
2
10
20
± 0.10
± 0.10
± 0.20
≤0.030
≤0.050
≤0.060
± 5.0
± 1.0
± 1.0
≤1.5
≤0.5
≤0.3
± 0.2
± 0.2
± 0.2
≤0.1
≤0.1
≤0.1
± 10.0
± 2.0
± 1.0
≤5.0
≤1.0
≤0.5
P100 (P/N F144057M) WITH D200 TIPS
10
50
100
± 0.35
± 0.40
± 0.80
≤0.10
≤0.12
≤0.15
± 3.5
± 0.8
± 0.8
≤1.0
≤0.24
≤0.15
± 0.8
± 0.8
± 0.8
≤0.3
≤0.3
≤0.3
± 8.0
± 1.6
± 0.8
≤3.0
≤0.6
≤0.3
P200 (P/N F144058M) WITH D200 TIPS
20
100
200
± 0.50
± 0.80
± 1.60
≤0.20
≤0.25
≤0.30
± 2.5
± 0.8
± 0.8
≤1.0
≤0.25
≤0.15
± 1.6
± 1.6
± 1.6
≤0.6
≤0.6
≤0.6
± 8.0
± 1.6
± 0.8
≤3.0
≤0.6
≤0.3
P1000 (P/N F144059M) WITH D1000 TIPS
100
500
1000
± 3.0
± 4.0
± 8.0
≤0.6
≤1.0
≤1.5
± 3.0
± 0.8
± 0.8
≤0.6
≤0.2
≤0.15
± 8.0
± 8.0
± 8.0
≤3.0
≤3.0
≤3.0
± 8.0
± 1.6
± 0.8
≤3.0
≤0.6
≤0.3
P5000*** (P/N F144066) WITH D5000 TIPS
500
2500
5000
± 12
± 15
± 30
≤3
≤5
≤8
± 2.4
± 0.6
± 0.6
≤0.6
≤0.2
≤0.16
± 40
± 40
± 40
≤15.0
≤15.0
≤15.0
± 8.0
± 1.6
± 0.8
≤3.0
≤0.6
≤0.3
P10mL*** (P/N F144067) WITH D10mL TIPS
1000
5000
10000
± 30
± 40
± 60
≤6
≤10
≤16
± 3.0
± 0.8
± 0.6
≤0.6
≤0.2
≤0.16
± 60
± 60
± 60
≤30.0
≤30.0
≤30.0
± 6.0
± 1.2
± 0.6
≤3.0
≤0.6
≤0.3
Gilson maximum permissible errors are only guaranteed when PIPETMAN® pipettes are used
with the recommended PIPETMAN® DIAMOND Tips.

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PIPETMAN® |QUICK GUIDEQUICK GUIDE |PIPETMAN®
PIPETMAN® MULTICHANNEL
Vol.
(µL)
Maximum Permissible Errors
Gilson ISO 8655
Systematic
Error
(µL)
Random
Error
(µL)
Systematic
Error
(%)
Random
Error
(%CV*)
Systematic
Error
(µL)
Random
Error
(µL)
Systematic
Error
(%)
Random
Error
(%CV*)
P8X10 (P/N F144068) AND P12X10 (P/N F144069) WITH D10 AND DL10 TIPS
1
5
10
± 0.08
± 0.20
± 0.20
≤0.05
≤0.10
≤0.10
± 8.0
± 4.0
± 2.0
≤5.0
≤2.0
≤1.0
± 0.24
± 0.24
± 0.24
≤0.16
≤0.16
≤0.16
± 24.0
± 4.8
± 2.4
≤16.0
≤.2
≤1.6
P8X20 (P/N F144070) AND P12X20 (P/N F144071) WITH DL10 AND D200 TIPS
2
10
20
± 0.10
± 0.20
± 0.40
≤0.08
≤0.10
≤0.20
± 5.0
± 2.0
± 2.0
≤4.0
≤1.0
≤1.0
± 0.4
± 0.4
± 0.4
≤0.20
≤0.20
≤0.20
± 20.0
± 4.0
± 2.0
≤10.0
≤2.0
≤1.0
P8X200 (P/N F144072) AND P12X200 (P/N F144073) WITH D200 TIPS
20
100
200
± 0.50
± 1.00
± 2.00
≤0.25
≤0.40
≤0.50
± 2.5
± 1.0
± 1.0
≤1.25
≤0.4
≤0.25
± 3.2
± 3.2
± 3.2
≤1.2
≤1.2
≤1.2
± 16.0
± 3.2
± 1.6
≤6.0
≤1.2
≤0.6
P8X300 (P/N F144074) AND P12X300 (P/N F144075) WITH D300 TIPS
30
150
300
± 1.00
± 1.50
± 3.00
≤0.35
≤0.60
≤1.00
± 3.33
± 1.0
± 1.0
≤1.17
≤0.4
≤0.33
± 8.0
± 8.0
± 8.0
≤3.0
≤3.0
≤3.0
± 26.67
± 5.33
± 2.67
≤10.0
≤2.0
≤1.0
Setting the Volume
The volume of liquid to be
aspirated is set using the
volume display. The dial colors
are either black or red to
indicate the position of the
decimal point, depending on
the model (refer to Figure 3).
The volume is set by turning
the thumbwheel or the push
button (refer to Figure 4). The
push button makes it easier and
faster to set volumes, especially when wearing gloves.
The thumbwheel may be turned using only one hand to
slowly reach the required setting.
Figure 3
Dial colors by model
1
5
P2
1.25 µL
2
0
7
5
P10
7.5 µL
1
2
5
P20
12.5 µL
P100
0
7
5
75 µL
P200
1
2
5
125 µL
P1000
0
7
5
0.75 mL
P5000
1
2
5
1.25 mL
P10mL
0
7
5
7.5 mL
0
7
5
Px10
7.5 µL
1
2
5
Px20
12.5 µL
Px200
1
2
5
125 µL
Px300
1
2
5
125 µL
To achieve maximum accuracy when setting the volume,
proceed as follows:
●When decreasing the
volume setting, slowly
reach the required setting,
making sure to not
overshoot the mark.
●When increasing the
volume setting, pass the
required value by 1/3 of
a turn and then slowly
decrease to reach the
volume, making sure to
not overshoot the mark.
Figure 4
Location of push button
and thumbwheel
Push button
Thumbwheel

6QUICK GUIDE |PIPETMAN®
Fitting the Tips
Single Channel Models
To fit a new PIPETMAN DIAMOND Tip, push the tip holder
into the tip using a slight twisting motion to ensure a firm,
airtight seal.
MODEL
COLOR OF VOLUMETER NUMBERS
BLACK RED INCREMENT
P2 µL 0.01 µL 0.002 µL
P10 - P20 - Px10 - Px20 µL 0.1 µL 0.02 µL
P100 - P200 - Px200 - Px300 µL - 0.2 µL
P1000 0.01 mL mL 0.002 mL
P5000 0.01 mL mL 0.002 mL
P10mL mL 0.1 mL 0.02 mL
Figure 5
PIPETMAN® DIAMOND Tip compatibility chart for
single channel models
PIPETMAN DIAMOND Tip
Compatibility for single channel
variable volume models
P2, P10 D10, DL10, DF10ST, DFL10ST
P20 D200, DF30ST
P100 D200, DF100ST
P200 D200, D300, DF200ST, DF300ST
P1000 D1000, D1200, DF1000ST, DF1200ST
P5000 D5000
P10mL D10mL
Fitting the Tips on P2 and P10 Models
NOTE
For the P2 and P10 models, a dual-
position adapter (plastic) is required
to fit DL10 tips (longtips) or D10 tips
(short tips). The metallic rod of the tip
ejector is shaped so the adapter may
be clipped to it in either position.
D5000 and D10mL PIPETMAN
DIAMOND tips do not have any filter.
P5000 and P10mL models are sold
with a bag of 10 filters. For more
details, refer to PIPETMAN User’s
Guide LT801122.
NOTICE

7
PIPETMAN® |QUICK GUIDEQUICK GUIDE |PIPETMAN®
Ejector Spacer for PIPETMAN® 10 µL
Multichannel Models
According to the tip used, D10 or DL10, you may have to
exchange the ejector spacer. The broadly spaced one is
for D10, and the narrowly spaced one is for DL10.
1. Remove the tip ejector, keeping both ejector locks
depressed. Pull the tip ejector down.
2. Gently press the tabs from the ejector spacer and
remove it from the tip
ejector.
3. Insert the alternative
ejector spacer and click
it to the tip ejector.
4. To refit the tip ejector,
gently re-insert the tip
ejector vertically into the
rails of the ejector
support.
P2 and P10 models are delivered with
the adapter in place, ready-to-use
DL10 tips. If D10 tips are used, the
adapter must be repositioned in the
shorter slot as follows:
1. Pull the adapter down from the
metallic rod.
2. Turn the adapter 180°.
3. Refit the adapter so the end of
the metallic rod engages the
shorter slot of the adapter.
NOTE The dual position is
autoclavable.
Figure 6
Dual-position
adapter for P2 and
P10 with stainless
steel tip ejector
Short tips Long tips
Ejector spacer
D10
Short tips
Long tips
Ejector
spacer
Figure 8
Ejector spacer location
PIPETMAN®DIAMOND Tips are best fitted with the
ROCKY RACK technique, invented by Gilson, available
only in our TIPACK and TOWERPACK.
Figure 7
ROCKY RACK
technique
PIPETMAN® DIAMOND Tip Compatibility
for Multichannel Models
P8x10, P12x10 D10*, DL10, DF10, DFL10
P8x20, P12x20 DL10, DFL10, DF30, D200
P8x200, P12x200 D200, D300, DF200, DF300
P8x300, P12x300 D200, D300, DF200, DF300
* Using the broad ejector spacer D10, you can adapt a
D10 tip (see above).
Multichannel models

8QUICK GUIDE |PIPETMAN®
Aspirating
1. Press the push button to the first stop
(this corresponds to the set volume of liquid).
2. Hold the pipette vertically and immerse the tip
in the liquid (refer to
PIPETMAN ® User’s Guide
LT801122 available on gilson.com) .
3. Release the push button slowly and smoothly (to
the top position) to aspirate the set volume of
liquid.
4. Wait one second, and then withdraw the pipette
tip from the liquid. You may wipe any droplets
away from the outside of the tip using a medical
wipe; however, if you do so, take care to avoid
touching the tip’s orifice.
First stop
Second stop
Figure 9
Pipetting motion - aspirate and dispense
Rest position
Pre-Wetting the Tips
Pre-wetting the tips before pipetting helps prepare the
tips for the best pipetting performance. Ideally, the pre-
wet includes both immersing the tip in the liquid and
performing one pipetting step.
Pre-wetting the tips helps ensure that volumes your
pipette’s volume will achieve accuracy and precision
within specifications.

9
PIPETMAN® |QUICK GUIDEQUICK GUIDE |PIPETMAN®
Maintenance
Routine maintenance will help keep your pipette in
good condition, ensuring a continued high level of
performance.
Maintenance is limited to cleaning or autoclaving
the parts specified in the
PIPETMAN ® User’s Guide
LT801122 , or replacing the push button, connecting nut,
tip ejector, tip holder, seal, and O-ring.
NOTICE
PIPETMAN® P2 and P10 should not be
disassembled. You may only replace the
push button, tip ejector, and its dual-position
adapter. With these pipettes, if the tip holder
is damaged, the piston may also be damaged.
After replacing any parts, you should verify
the performance of your pipette following the
verification procedure available on the Gilson
website (www.gilson.com). If the pipette needs
to be readjusted, please contact your local
authorized Gilson Service Center.
Dispensing
1. Place the end of the tip against the inside wall of the
recipient vessel (at an angle of 10° to 40°).
2. Press the push button slowly and smoothly to the
first stop.
3. Wait for at least one second, then press the push
button to the second stop to expel any residual
liquid from the tip. Keep the push button pressed
fully down and (while removing the pipette) draw
the tip along the inside surface of the vessel.
4. Release the push button smoothly. Eject the tip by
pressing firmly on the tip ejector button.
It is recommended to regularly verify the volume
displayed on the dial while pipetting.
NOTICE
NOTICE
Decreased pipetting forces are due to the
design of the piston assembly, which includes
a very high-quality lubricant (part number
5440011070). The use of any other lubricant
voids the warranty of this pipette.
NOTICE
Gilson recommends maintenance and
calibration at least annually, more frequently as
needed, depending on use.
Cleaning and Decontamination
PIPETMAN is designed so the parts normally in contact
with liquid contaminants can easily be cleaned and
decontaminated.
For further information, please refer to PIPETMAN User’s
Guide LT801122 available on gilson.com.

10 QUICK GUIDE |PIPETMAN®
Warranty
Gilson warrants this pipette against defects in material
under normal use and service for a period of three years
for both single channel and multichannel models from
the date of purchase.
This warranty shall not apply to pipettes which are
subject to abnormal use and/or improper or inadequate
maintenance (contrary to the recommendations given in
the user’s guide), including, but not limited to pipettes
that have been subjected to physical damage, improper
handling, or spillage or exposure to any corrosive
environment. This warranty shall also be void in the
event pipettes are altered or modified by any party
other than Gilson or its designates. Gilson’s sole liability
under this warranty shall be limited to, at Gilson’s
sole option, repair or replacement of any defective
components of pipettes or refund of the purchase price
paid for such pipettes. Routine cleaning, control and
recalibration are not covered under the warranty. The
replacement of wearing parts such as seals, O-rings,
broken pistons assembly, and broken tip holders are
not covered under the warranty.
NOTICE
Yearly routine maintenance is highly
recommended to keep your pipette in good
condition, ensuring a continued high level of
performance.
THE FOREGOING WARRANTY IS EXCLUSIVE
AND GILSON HEREBY DISCLAIMS ALL OTHER
WARRANTIES, WHETHER EXPRESS OR
IMPLIED, INCLUDING ANY WARRANTIES OF
MERCHANTABILITY AND ANY WARRANTIES OF
FITNESS FOR A PARTICULAR PURPOSE, UNDER
NO CIRCUMSTANCES SHALL GILSON BE LIABLE
FOR ANY CONSEQUENTIAL, PUNITIVE, INDIRECT
OR INCIDENTAL DAMAGES ARISING OUT OF ANY
BREACH OF ANY EXPRESS OR IMPLIED WARRANTY.
Web Resources
Visit Gilson’s website at www.gilson.com for:
●Service contact information
●PIPETMAN® User’s Guide LT801122 pdf
●Information about accessories, spare parts, and
related products
●Additional product information and special offers

11
PIPETMAN® |QUICK GUIDEQUICK GUIDE |PIPETMAN®
NOTES

gilson.com/contactus
LT801624/E | ©2023 Gilson, Inc. All rights reserved.