Planer Kryo250 User manual

Kryo250
Operators' Manual

© 2004 Planer plc.
Planer plc
110 Windmill Road
Sunbury-on-Thames
Middlesex
U.K.
TW16 7HD
Telephone [phone]
Facsimile [phone]
Kryo 250 Operators’ Manual MA009478
Issue 1.0 July 2004

Notices
This equipment contains batteries and other components which have a limited
life. To ensure the long life of the equipment and help ensure trouble-free
operation, we recommend that you join one of our Maintenance and Support
Schemes. Please contact your supplier for details.
Good practice demands that in critical applications, reliance should not be
placed on a single source of data. Hard copies and backup copies should be
routinely kept.
All equipment supplied by Planer plc. is subject to a Warranty Agreement and
Limited Liability. In addition, software products are not sold but are licensed
for use and are subject to a Licence Agreement. Copies of these documents will
have been provided with the equipment or as part of the software installation.
Please ensure that you read these documents before using the equipment or
software. If you cannot locate this documentation, please contact your supplier
who will be able to provide you with copies.
Planer plc reserve the right to alter products and their specifications without
notice.
i

Symbols Used in this Manual
Failure to follow these instructions could result in personal
injury.
Failure to follow these instructions could result in damage to
equipment or samples.
This indicates first-aid advice.
Symbols Used on the Equipment
Caution: Refer to this manual and accompanying
documentation
Mains supply ON
COOL manual override
HEAT manual override
Display sample temperature
STOP key
RUN key
Alternating current
Mains supply OFF
ii

SAFETY PRECAUTIONS
IT IS IMPORTANT TO READ THIS SAFETY NOTICE PRIOR TO
OPERATING ANY EQUIPMENT
First Aid
IF IN DOUBT, SEEK IMMEDIATE MEDICAL
ATTENTION. If any quantity of liquid nitrogen comes
into contact with the skin or eyes, immediately flood that
area of the body with large quantities of unheated water,
apply cold compresses and seek medical attention. If the
skin is blistered or there is any possibility that eyes have
been affected, the patient should be taken immediately to
a doctor or hospital for treatment.
Safety
Failure to comply with these instructions could result
in personal injury
Operating the system in a manner not specified within in this manual or
under conditions outside of the specifications, see Appendix A, may result
in the protection offered by the equipment being impaired.
Avoid nitrogen build up: anywhere liquid nitrogen is used must be well
ventilated to reduce the risk of nitrogen build up. Small volumes of liquid
nitrogen convert to very large volumes of gas. This can result in
drowsiness, or in extreme cases, asphyxiation.
When liquid nitrogen is used in confined spaces, oxygen-deficiency alarms
must be installed. Guidance on ventilation requirements are given in
BCGA Code of Practice CP30, The safe use of liquid nitrogen dewars up to
50 litres.
When there is a risk of reduced or insufficient ventilation when a room is
unoccupied, then alarms to indicate oxygen deficiency must be installed
and positioned outside of the room so that operators are aware of the
hazard before entering. The operational status of such an alarm should also
be visible from outside the room.
iii

If liquid nitrogen is used in an area that requires forced ventilation, an
alarm to indicate its failure should be fitted.
When dispensing or potentially being exposed to liquid nitrogen, protect
the face with a shield and wear gloves, boots and a protective apron.
Prevent spillage into shoes and onto unprotected parts of the body.
Handle vessels containing liquid nitrogen carefully. Liquid nitrogen boils at
-196ºC and both liquid and gas can cause rapid and severe frostbite.
Delicate tissue, e.g. the eyes, can be damaged by an exposure to the cold
gas, which may be too brief to affect the skin of the hands or face.
Stand clear of boiling and splashing liquid nitrogen and its gas when filling
a dewar at normal room temperature or when inserting objects (such as a
pump) into the liquid.
Use tongs or wear cryogloves when handling cold or hot objects.
Cryogloves are available as an accessory from Planer plc. When running a
program, the chamber and contents may get cold enough to cause frostbite.
The dewar must be depressurised before the pump can be removed. This is
achieved by opening the pressure-relief valve mounted on the side of the
pump, which seals an orifice in the pressure system by toggle action. Avoid
contact with cold nitrogen gas when depressurising the dewar.
The dewars used with this equipment must never be used to store any liquid
other than liquid nitrogen.
Only use vessels designed for working with liquid nitrogen.
Secure the liquid nitrogen container to prevent toppling.
Ensure that the delivery pipe connections are secure and leak-free before
supplying liquid nitrogen to the freezer.
Liquid nitrogen pressure must not exceed 1.7 bar (25 psi).
To maintain protection against electric shock, the mains lead must be
properly fitted to a 3-way mains connector plugged into an earthed mains
outlet.
Because of the potentially wet laboratory environment, the operator should
be provided with additional protection against electric shock by supplying
mains power to the freezer through a residual current circuit breaker
(RCCB) operating at a differential of 30 mA. Note that care must be taken
iv

to ensure that the freezer is not compromised by faulty equipment sharing
the same supply and causing nuisance trips.
The mains outlets on the chamber’s rear panel are only for use with the
Planer LNP4 unit and the Kryo250 Controller.
The 9-way plug on the Controller is only for connecting to the RS232
serial port of a computer that complies with IEC950.
The 37-way socket on the Controller is only to be used for connecting to
the chamber's cable.
The AUX socket on the Controller is to be used only for calibration by
qualified service personnel. It must not be connected to any other
equipment.
The Sample PRT socket on the chamber's rear panel is only to be used
with a Planer Sample PRT.
When heating under manual control, check the chamber temperature at
intervals of 30 seconds or less to avoid overheating.
User servicing is limited to cleaning and decontamination. All other
servicing must only be undertaken by suitably qualified engineers.
To avoid risk of fire, fuses must always be replaced with the same type and
rating.
Do not move the chamber whilst still wet after a freezing run.
v

EMC AND ESD CONSIDERATIONS
The following precautions must be taken to ensure that the
equipment is not damaged by electrostatic discharge (ESD),
and that its immunity to radio frequency interference is not
compromised.
Ensure that the Controller is not running a program and that the mains
power is disconnected before connecting or disconnecting any cable.
Fit the plastic covers and plugs provided to all unused connectors.
Immediately before touching the sample PRT, touch an earthed part, such
as the metal surface at the rear of the Controller.
Do not touch any uncovered connectors with your hands or tools, even
when the equipment is switched off.
When connecting the system to a PC via the 9 way plug, always use a fully
screened cable no longer than 2m.
Take care to avoid placing freezers in environments influenced by sources
of electromagnetic interference, such as cyclotrons, large transformers etc.
vi

Contents
3-2The Run has Stopped Prematurely ..........
3-2Troubleshooting ..............................
3-2Calibrating the System .........................
3-1Routine Maintenance ..........................
3-1
Routine Maintenance and
Troubleshooting
Chapter 3
........................
2-6Unexpected Reset .............................
2-6Power Failures ...............................
2-6Shutting Down the System ................
2-5Warming the Chamber ...................
2-5Stopping a Run .........................
2-5Removing the Samples ...................
2-5Loading the Samples ....................
2-4Starting the Run ........................
2-4Freezing Samples .............................
2-3Controller Rear Panel ..........................
2-2Controller Front Panel .........................
2-1Chamber Front Panel ..........................
2-1
Operating the Kryo250Chapter 2 .................
1-8Loading Printer Paper .........................
1-7Removing the Pump .....................
1-7Testing the Dewar .......................
1-6Fitting the Pump ........................
1-5Filling the Dewar .......................
1-5Connecting the Delivery Hose .............
1-5Installing a Planer Pump and Dewar ..............
1-4Installing a CryoCyl Low Pressure Cylinder ........
1-3Installing the Kryo250 .........................
1-2Rear Connections .......................
1-1Top View .............................
1-1Overview of the System ........................
1-1
Installing the SystemChapter 1 ...................
viEMC and ESD Considerations .............
iiiSafety Precautions ............................
iiSymbols Used on the Equipment .................
iiSymbols Used in this Manual ...................
i
N
otices .....................................
vii

B-1
Safety DeclarationAppendix B
A-2Fuses .......................................
A-2Chamber Specifications ........................
A-1Controller Specifications .......................
A-1System Specifications .........................
A-1
SpecificationsAppendix A ...........................
3-4Returning for Service ..........................
3-3No Response from the System .............
3-3The Chamber will not Heat ...............
3-3The Chamber will not Cool ...............
viii

Chapter 1
Installing the System
Overview of the System
An overview of the system and connections is given in the following diagrams.
Top View
In the above diagram the chamber is shown in its vertical position. The
chamber can be placed horizontally if preferred.
Installing the System 1 - 1
Hose connection
to liquid nitrogen
RS232 port
Aux socket
37 way
cable
Mains power inlet Power outlet for
LNP4 pump
supply
Sample PRT socket
Chamber
Controller
Power to
controller

Rear Connections
1 - 2 Installing the System
Sample PRT
Mains power
Do not connect
until instructed
to do so in the
manual.
Power to LNP4 pump
if used
Power from
chamber to
controller
Manual override
Reset button
AUX socket
RS232 to PC
37 way D Type
connection to
chamber
Liquid
nitrogen
inlet
Rear panel of chamber Rear panel of controller

Installing the Kryo250
Carefully unpack the equipment.
Place the Kryo250 Controller and the Chamber on a secure, flat surface.
Connect the 37 way D Type socket from the Chamber to the plug on the
rear of the Controller.
Connect the sample platinum resistance thermometer (PRT) into the PRT
socket on the rear of the Chamber.
The 9-way plug on the Controller is only for connecting to
the serial port of a computer which complies with IEC950
When connecting the system to a PC via the 9 way plug,
always use a fully screened cable no longer than 2m.
If connecting to a PC, connect the 9 way D type plug on the rear of the
Controller to an RS232 port on the PC using the one to one cable supplied.
If using a Planer pump and Dewar refer to the instructions under Installing
a Planer Pump and Dewar or if using a CryoCyl low pressure cylinder,
refer to the instructions under Installing a CryoCyl Low Pressure Cylinder.
After connecting the liquid nitrogen supply, connect the mains inlet at the
rear of the Chamber, to a suitable wall socket.
Switch on the system and wait for the display on the Controller to complete
its self-check.
Installing the System 1 - 3

Installing a CryoCyl Low Pressure Cylinder
Wear protective clothing, including cryogloves, goggles,
boots and a protective apron.
The liquid-nitrogen supply pressure must not exceed 1.7
bar (25 psi).
The Controller is optimised for use with a Planer pump and
dewar ruuning at approximately 0.5 bar (7.5 psi). If
running from a 1.5 bar (22 psi) cylinder, alternative control
coefficients should be used. These can be downloaded into
the controller using a PC connection. Please contact your
supplier for details.
If the hose is not already fitted to the Chamber, wrap the male hose thread
with PTFE tap and fix to the LN
2
input on the back of the chamber.
Tighten securely with a spanner.
Connect an appropriate adapter to the cylinder, observing the
manufacturer’s instructions, then connect the delivery tube to the adapter
ensuring that the fibre washer is fitted to this joint. Tighten securely with a
spanner.
After connecting both ends of the delivery tube, open the
liquid-nitrogen tap slowly and check for leaks.
1 - 4 Installing the System

Installing a Planer Pump and Dewar
Connecting the Delivery Hose
Never insert the pump into a dewar until it has been
connected to the chamber.
To avoid damage to the heating element, support the pump
carefully while it is out of the dewar.
If the hose is not already fitted to the Chamber, wrap the male hose thread
with PTFE tap and fix to the LN
2
input on the back of the chamber.
Tighten securely with a spanner.
Fit the female ferrule at the other end of the hose, to the outlet on the side
of the pump. Ensure that the fibre washer is fitted to this joint. Tighten
securely with a spanner.
Filling the Dewar
When refilling a dewar that already contains liquid
nitrogen, you must first depressurise it and remove the
pump as described in Removing the pump.
Wear protective clothing, including cryogloves, goggles,
boots and a protective apron.
Only fill the dewar with liquid nitrogen and do not exceed
85% of its capacity.
Fully insert the hose from the main liquid nitrogen storage container into
the dewar to avoid spillage.
Add the liquid nitrogen slowly into the dewar. Cold gas will issue from the
dewar. Use a dipstick to ensure that the dewar is filled to between 50% and
85% of its capacity.
Installing the System 1 - 5

Fitting the pump
Wear protective clothing, including cryogloves, goggles,
boots and a protective apron.
Ensure that the red pressure relief valve on the pump is
open.
Ensure that power is disconnected from the chamber and
the pump.
Ensure the delivery hose from the pump to the chamber is
securely tightened.
Make sure that the delivery hose is as straight as possible, with no major
high or low spots.
Do not insert the pump rapidly as the liquid nitrogen may
bubble violently.
In one smooth and continuous action, insert the pump evenly into the neck
of the dewar until the microswitch plunger operates on the neck ring. It is
normal for nitrogen gas to issue from the pressure-relief valve.
Press firmly on the top of the pump to hold it level and clip all the springs
onto the dewar, alternating between handles.
Close the pressure-relief valve.
The mains power outlet on the rear of the Chamber must
only be used for connection to a Planer Products pump.
Connect the mains inlet of the pump power supply unit to the auxiliary
power output on the rear of the Chamber.
Always test the dewar after refitting the pump.
1 - 6 Installing the System

Testing the Dewar
Wear protective clothing, including cryogloves, goggles,
boots and a protective apron.
Connect the mains lead from the Chamber to a appropriate wall socket.
Switch on the Kryo250 system.
Press the switch on the pump power supply unit. The adjacent light will
illuminate. After about 5 minutes, the pressure will rise to 5 psi and the
light will switch off. The dewar should remain pressurised until almost
empty.
Before rectifying any leaks, or to shutdown the system,
switch off the Kryo250 system, disconnect the mains
supply at the wall socket, open the red pressure-relief valve
and wait for the flow of gas to stop.
Check for liquid and gas leaks.
Removing the Pump
You will need to remove the pump whenever the dewar needs to be refilled.
Never remove a pump from a dewar until the pressure
gauge reading has fallen to 0 psi.
Wear protective clothing, including cryogloves, goggles,
boots and a protective apron.
Switch off the freezer.
Disconnect the mains supply at the wall socket.
Open the red pressure-relief valve. Cold gas will issue from the valve.
Wait until the pressure on the gauge has fallen to zero and the flow of gas
has stopped.
Disconnect the pump from its power supply unit.
Installing the System 1 - 7

Press firmly on the upper surface of the pump, then remove the spring clips
from the handles on the dewar. To prevent jamming only remove one
spring at a time from each side.
Lift the pump carefully, but firmly, out of the neck of the dewar using a
slight twisting and rocking action.
The end of the pump may be cold enough to cause frostbite.
Place the pump in a safe position on its side or suspended vertically.
Remove frost using a hot-air gun.
Do not overheat as the plastic parts or thermal cut-out may
be damaged.
When the pump has attained ambient temperature, dry any condensation with
absorbent tissue to prevent ice from blocking the filter when the pump is
refitted.
Fit a bung to the dewar to prevent water condensing inside.
1 - 8 Installing the System

Chapter 2
Operating the Kryo250
Chamber Front Panel
Operating the Kryo250 2 - 1
Indicates when
chamber is being
cooled
Indicates when
chamber is being
heated
Mains on off
switch

Controller Front Panel
2 - 2 Operating the Kryo250
-120.4
C
Chamber temperature
(or sample temperature while
sample button is depressed).
EEEE indicates that the chamber
or sample PRT is faulty. A profile
can be run without a working
sample PRT but the chamber
PRT must be in full working
order.
- - - - indicates a stuck button.
S indicates that samples are
ready for seeding if this feature
is selected.
Sample temperature button
Keep depressed to display the
sample temperature.
Indicator light
Lit when running a program.
Flashes for operator attention.
Run
Press to start a profile or after
manual seeding
Stop
Press to stop a profile